SitesBLAST

SitesBLAST – Find functional sites

SitesBLAST

Comparing BPHYT_RS33330 BPHYT_RS33330 C4-dicarboxylate ABC transporter to proteins with known functional sites using BLASTp with E ≤ 0.001.

Or try Sites on a Tree, PaperBLAST, Conserved Domains, or compare to all protein structures

Found 20 (the maximum) hits to proteins with known functional sites (download)

O59010 Glutamate transporter homolog; Glt(Ph); Sodium-aspartate symporter Glt(Ph); Sodium-dependent aspartate transporter from Pyrococcus horikoshii (strain ATCC 700860 / DSM 12428 / JCM 9974 / NBRC 100139 / OT-3) (see 3 papers)
31% identity, 92% coverage: 9:407/435 of query aligns to 16:418/425 of O59010

query
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O59010

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S65 (≠ T62) mutation to V: Strongly decreased chloride conductance.
R276 (≠ A271) mutation to S: Increased rate of aspartate transport; when associated with R-395.
RSS 276:278 (≠ ASS 271:273) binding
M311 (≠ L306) mutation to A: Decreased dependence of aspartate binding on Na(+) concentration.
T314 (≠ S309) binding
V355 (= V350) binding
D394 (= D383) binding
M395 (= M384) mutation to R: Increased rate of aspartate transport; when associated with S-276.
R397 (= R386) mutation to A: Strongly decreased affinity for aspartate.
N401 (= N390) binding
D405 (≠ N394) mutation to N: Strongly decreased affinity for aspartate.

2nwwA Crystal structure of gltph in complex with tboa (see paper)
32% identity, 91% coverage: 9:405/435 of query aligns to 7:407/407 of 2nwwA

query
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binding (3s)-3-(benzyloxy)-l-aspartic acid: S269 (= S273), T305 (≠ S309), A349 (≠ G353), G350 (≠ S354), D385 (= D383), R388 (= R386), T389 (= T387), N392 (= N390)

6bauA Crystal structure of gltph r397c in complex with l-cysteine (see paper)
31% identity, 91% coverage: 9:405/435 of query aligns to 8:408/408 of 6bauA

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A

V

I

I

V

A

A

K

K

W

T

E

E

binding cysteine: S270 (= S273), M303 (≠ L306), T306 (≠ S309), A345 (= A348), G346 (= G349), V347 (= V350), G351 (≠ S354), D386 (= D383), C389 (≠ R386), T390 (= T387), N393 (= N390)

6x15A Inward-facing state of the glutamate transporter homologue gltph in complex with l-aspartate and sodium ions (see paper)
32% identity, 91% coverage: 9:405/435 of query aligns to 16:416/419 of 6x15A

query
sites
6x15A

I

I

V

L

A

I

G

G

M

L

A

I

L

L

G

G

V

A

I

I

V

V

G

G

Y

L

V

I

C

L

-

G

H

H

R

Y

S

G

A

Y

A

A

G

H

A

A

A

V

E

H

A

T

K

-

T

-

I

-

A

-

G

-

Y

Y

F

V

S

K

I

P

I
x
F

T

G

D

D

I

L

F

F

L

V

R

R

L

L

V

L

K

C

M

M

I

L

I

V

A

M

P

P

L

I

V

V

F

F

A

A

T

S

L

L

V

V

S

V

G

G

L

A

A

A

G

S

M

I

E

S

G

-

T
x
P

S

A

D

R

V

L

R

G

R

R

I

V

G

G

F

V

R

K

S

I

V

V

G

V

W

Y

F
x
Y

V

L

C
x
L

A
x
T

S
|
S

L

A

F
|
F

S

A

L

V

A

T

L

L

G

G

L

I

A

I

L

M

A

A

N

R

A

L

L

F

Q

N

P

P

G

G

A

A

G

G

L

I

H

H

M

-

T

-

Q

-

T

-

S

-

D

-

V

-

A

-

T

-

G

-

L

L

N

A

T

V

A

G

G

G

L

Q

N

Q

F

F

K

Q

D

P

F

H

-

Q

-

A

-

P

V
x
L

T

V

H

H

A
x
I

F

L

-

L

-

D

-

I

-

V

P

P

S

T

S

N

I

P

I

F

D

G

A

A

M

L

A

A

R

N

N

G

D

Q

I

V

L

L

Q

P

I

T

L

I

V

F

F

F

S

A

V
x
I

L

I

F

L

G

G

V

I

V

A

L

I

S

T

A
x
Y

I

L

-

M

-

N

-

S

-

E

-

N

-

E

K

K

K

V

D

R

P

K

R

S

V

A

T

E

P

T

L

L

I

L

A

D

G

A

I

I

D

N

A

G

L

L

V

A

P

E

A

A

M

M

L

Y

K
|
K

L

I

T

V

D

N

Y
x
G

V

V

M

M

R

Q

L

Y

A

A

P

P

I
|
I

G

G

V

V

F
|
F

G

A

A

L

L

I

A

A

S

Y

A

V

I

M

T

A

V

E

N

Q

G

G

L

V

D

H

V

V

L

V

T

G

T

E

Y

L

G

A

K

K

L

V

V

T

G

A

S

A

F

V

Y

Y

L

V

G

G

L

L

V

-

T

T

L

L

W

Q

I

I

V

L

L

L

I

V

-

Y

F

F

V

V

G
x
L

Y

L

A

K

F

I

L

Y

G

G

K

I

S

D

I

P

W

I

R

S

L

F

L
x
I

K

K

A

H

V

A

R

K

E

D

P

A

A
x
M

M

L

L

T

A

A

F

F

S

V

T

T

A

R

S
|
S

E

S

A

G

A

T

Y

L

P

P

R

V

L

T

T

M

E

R

K

V

L

A

E

K

A

E

F

M

G

G

I

I

D

S

K

E

K

G

V

I

V

Y

G

S

F

F

T

T

L

L

P

P

L

L

G
|
G

Y

A

A
x
T

F

I

N
|
N

L
x
M

D
|
D

G

G

S
x
T

M

A

M

L

Y

Y

Q

Q

A

G

F

V

A

A

A

T

I

F

F

F

I

I

A

A

Q

N

A

A

F

L

G

G

I

S

D

H

M

L

P
x
T

L
x
V

G
|
G

A

Q

Q

Q

I

L

M
x
T

M

I

L

V

L
|
L

V

T

L

A

M

V

L

L

S

A

S
|
S

K
x
I

G

G

M
x
T

A

A

G
|
G

V

V

A

P

R

G

G
x
A

S
x
G

V

A

V

I

V

M

V

L

A

C

A

M

I

V

A

L

P

H

M

S

F

V

H

G

L

L

P

P

-

L

-

T

-

D

-

P

-

N

-

V

P

A

S

A

G

A

V

Y

V

A

L

M

I

I

L

L

A

G

I

I

D

D

Q

A

I

I

L

L

D
|
D

M

M

G

G

R
|
R

T
|
T

A
x
M

T

V

N
|
N

V
|
V

I

T

G

G

N
x
D

S

L

I

T

A

G

T

T

A

A

V

I

I

V

A

A

K

K

W

T

E

E

binding [(2~{R})-1-[2-azanylethoxy(oxidanyl)phosphoryl]oxy-3-hexadecanoyloxy-propan-2-yl] (~{Z})-octadec-9-enoate: F46 (≠ I43), F46 (≠ I43), P75 (≠ T73), L91 (≠ C89), F95 (= F93), L130 (≠ V135), I133 (≠ A138), I159 (≠ V160), Y167 (≠ A168), K196 (= K191), G200 (≠ Y195), I207 (= I202), F210 (= F205), L250 (≠ G245), I262 (≠ L257), M269 (≠ A264), T334 (≠ P329), V335 (≠ L330), G336 (= G331), T340 (≠ M335), L343 (= L338), M399 (≠ A388)
binding aspartic acid: S277 (= S272), S278 (= S273), T314 (≠ S309), G354 (= G349), A358 (≠ G353), G359 (≠ S354), D394 (= D383), R397 (= R386), T398 (= T387)
binding sodium ion: Y89 (≠ F87), T92 (≠ A90), S93 (= S91), G306 (= G301), T308 (≠ A303), N310 (= N305), N310 (= N305), M311 (≠ L306), D312 (= D307), S349 (= S344), I350 (≠ K345), T352 (≠ M347), N401 (= N390), V402 (= V391), D405 (≠ N394)

Sites not aligning to the query:

binding [(2~{R})-1-[2-azanylethoxy(oxidanyl)phosphoryl]oxy-3-hexadecanoyloxy-propan-2-yl] (~{Z})-octadec-9-enoate: 4, 7

6bavA Crystal structure of gltph r397c in complex with s-benzyl-l-cysteine (see paper)
31% identity, 91% coverage: 9:405/435 of query aligns to 8:408/409 of 6bavA

query
sites
6bavA

I

I

V

L

A

I

G

G

M

L

A

I

L

L

G

G

V

A

I

I

V

V

G

G

Y

L

V

I

C

L

-

G

H

H

R

Y

S

G

A

Y

A

A

G

D

A

A

A

V

E

K

A

T

K

-

T

-

I

-

A

-

G

-

Y

Y

F

V

S

K

I

P

I

F

T

G

D

D

I

L

F

F

L

V

R

R

L

L

V

L

K

K

M

M

I

L

I

V

A

M

P

P

L

I

V

V

F

F

A

A

T

S

L

L

V

V

S

V

G

G

L

A

A

A

G

S

M

I

E

S

G

-

T

P

S

A

D

R

V

L

R

G

R

R

I

V

G

G

F

V

R

K

S

I

V

V

G

V

W

Y

F

Y

V

L

C

L

A

T

S

S

L

A

F

F

S

A

L

V

A

T

L

L

G

G

L

I

A

I

L

M

A

A

N

R

A

L

L

F

Q

N

P

P

G

G

A

A

G

G

L

I

H

H

M

-

T

-

Q

-

T

-

S

-

D

-

V

-

A

-

T

-

G

-

L

L

N

A

T

V

A

G

G

G

L

Q

N

Q

F

F

K

Q

-

P

-

K

-

Q

-

A

-

P

-

L

-

V

D

K

F

I

V

L

T

L

H

D

A

I

F

V

P

P

S

T

S

N

I

P

I

F

D

G

A

A

M

L

A

A

R

N

N

G

D

Q

I

V

L

L

Q

P

I

T

L

I

V

F

F

F

S

A

V

I

L

I

F

L

G

G

V

I

V

A

L

I

S

T

A

Y

I

L

-

M

-

N

-

S

-

E

-

N

-

E

K

K

K

V

D

R

P

K

R

S

V

A

T

E

P

T

L

L

I

L

A

D

G

A

I

I

D

N

A

G

L

L

V

A

P

E

A

A

M

M

L

Y

K

K

L

I

T

V

D

N

Y

G

V

V

M

M

R

Q

L

Y

A

A

P

P

I

I

G

G

V

V

F

F

G

A

A

L

L

I

A

A

S

Y

A

V

I

M

T

A

V

E

N

Q

G

G

L

V

D

K

V

V

L

V

T

G

T

E

Y

L

G

A

K

K

L

V

V

T

G

A

S

A

F

V

Y

Y

L

V

G

G

L

L

V

-

T

T

L

L

W

Q

I

I

V

L

L

L

I

V

-

Y

F

F

V

V

G

L

Y

L

A

K

F

I

L

Y

G

G

K

I

S

D

I

P

W

I

R

S

L

F

L

I

K

K

A

K

V

A

R

K

E

D

P

A

A

M

M

L

L

T

A

A

F

F

S

V

T

T

A
x
R

S

S

S
|
S

E

S

A

G

A

T

Y

L

P

P

R

V

L

T

T

M

E

R

K

V

L

A

E

K

A

E

F

M

G

G

I

I

D

S

K

E

K

G

V

I

V

Y

G

S

F

F

T

T

L

L

P

P

L

L

G

G

Y

A

A

T

F

I

N

N

L

M

D

D

G

G

S
x
T

M

A

M

L

Y
|
Y

Q

Q

A

G

F

V

A

C

A

T

I

F

F

F

I

I

A

A

Q

N

A

A

F

L

G

G

I

S

D

H

M

L

P

T

L

V

G

G

A

Q

Q

Q

I

L

M

T

M

I

L

V

L

L

V

T

L

A

M

V

L

L

S

A

S

S

K

I

G

G

M

T

A

A

G

G

V

V

A

P

R

G

G

A

S

G

V

A

V

I

V

M

V

L

A

A

A

M

I

V

A

L

P

E

M

S

F

V

H

G

L

L

P

P

-

L

-

T

-

D

-

P

-

N

-

V

P

A

S

A

G

A

V

Y

V

A

L

M

I

I

L

L

A

G

I

I

D
|
D

Q

A

I

I

L

L

D
|
D

M

M

G

G

R
x
C

T
|
T

A

M

T

V

N

N

V

V

I

T

G

G

N

D

S

L

I

T

A

G

T

T

A

A

V

I

I

V

A

A

K

K

W

T

E

E

binding benzylcysteine: R268 (≠ A271), S270 (= S273), T306 (≠ S309), Y309 (= Y312), D382 (= D379), D386 (= D383), C389 (≠ R386), T390 (= T387)

6x14A Inward-facing state of the glutamate transporter homologue gltph in complex with tfb-tboa (see paper)
32% identity, 91% coverage: 9:405/435 of query aligns to 13:413/413 of 6x14A

query
sites
6x14A

I
|
I

V

L

A

I

G

G

M

L

A

I

L

L

G

G

V

A

I

I

V

V

G

G

Y

L

V

I

C

L

-

G

H

H

R

Y

S

G

A

Y

A

A

G

H

A

A

A

V

E

H

A

T

K

-

T

-

I

-

A

-

G

-

Y

Y

F

V

S

K

I

P

I

F

T

G

D

D

I

L

F

F

L

V

R

R

L

L

V

L

K

C

M

M

I

L

I

V

A

M

P

P

L

I

V

V

F

F

A

A

T

S

L

L

V

V

S

V

G
|
G

L

A

A

A

G

S

M

I

E

S

G

-

T

P

S

A

D

R

V

L

R

G

R

R

I

V

G

G

F

V

R

K

S

I

V
|
V

G

V

W

Y

F

Y

V

L

C

L

A

T

S

S

L

A

F

F

S

A

L

V

A

T

L

L

G

G

L

I

A

I

L

M

A

A

N

R

A

L

L

F

Q

N

P

P

G

G

A

A

G

G

L

I

H

H

M

-

T

-

Q

-

T

-

S

-

D

-

V

-

A

-

T

-

G

-

L

L

N

A

T

V

A

G

G

G

L

Q

N

Q

F

F

K

Q

D

P

F

H

-

Q

-

A

-

P

V

L

T

V

H

H

A

I

F

L

-

L

-

D

-

I

-

V

P

P

S

T

S

N

I

P

I

F

D

G

A

A

M

L

A

A

R

N

N

G

D

Q

I

V

L

L

Q

P

I

T

L

I

V

F

F

F

S

A

V

I

L
x
I

F

L

G

G

V

I

V

A

L

I

S

T

A
x
Y

I

L

-

M

-

N

-

S

-

E

-

N

-

E

K

K

K

V

D

R

P

K

R

S

V

A

T

E

P

T

L

L

I

L

A

D

G

A

I

I

D

N

A

G

L

L

V

A

P

E

A

A

M

M

L

Y

K
|
K

L

I

T

V

D

N

Y

G

V

V

M
|
M

R

Q

L

Y

A

A

P

P

I

I

G

G

V

V

F

F

G

A

A

L

L

I

A

A

S

Y

A

V

I

M

T

A

V

E

N

Q

G

G

L

V

D

H

V

V

L

V

T

G

T

E

Y

L

G

A

K

K

L

V

V

T

G

A

S

A

F

V

Y

Y

L

V

G

G

L

L

V

-

T

T

L

L

W

Q

I

I

V

L

L

L

I

V

-

Y

F

F

V

V

G

L

Y

L

A

K

F

I

L

Y

G

G

K

I

S

D

I

P

W

I

R

S

L

F

L

I

K

K

A

H

V

A

R

K

E

D

P

A

A

M

M

L

L

T

A

A

F

F

S

V

T

T

A

R

S

S

S
|
S

E

S

A

G

A

T

Y

L

P

P

R

V

L

T

T

M

E

R

K

V

L

A

E

K

A

E

F

M

G

G

I

I

D

S

K

E

K

G

V

I

V

Y

G

S

F

F

T

T

L

L

P

P

L

L

G

G

Y

A

A
x
T

F
x
I

N

N

L

M

D

D

G

G

S
x
T

M

A

M

L

Y

Y

Q

Q

A

G

F

V

A

A

A

T

I

F

F

F

I

I

A

A

Q

N

A

A

F

L

G

G

I

S

D

H

M

L

P

T

L

V

G

G

A

Q

Q

Q

I

L

M

T

M

I

L

V

L

L

V

T

L

A

M

V

L

L

S

A

S

S

K
x
I

G

G

M

T

A

A

G

G

V

V

A

P

R

G

G

A

S
x
G

V

A

V

I

V

M

V

L

A

C

A

M

I

V

A

L

P

H

M

S

F

V

H

G

L

L

P

P

-

L

-

T

-

D

-

P

-

N

-

V

P

A

S

A

G

A

V

Y

V

A

L

M

I

I

L
|
L

A

G

I

I

D

D

Q

A

I

I

L

L

D
|
D

M

M

G

G

R
|
R

T

T

A

M

T

V

N

N

V

V

I

T

G

G

N

D

S

L

I

T

A

G

T

T

A

A

V

I

I

V

A

A

K

K

W

T

E

E

binding [(2~{R})-1-[2-azanylethoxy(oxidanyl)phosphoryl]oxy-3-hexadecanoyloxy-propan-2-yl] (~{Z})-octadec-9-enoate: G66 (= G66), V83 (= V84), I157 (≠ L161), Y164 (≠ A168), K193 (= K191), T305 (≠ A303), I306 (≠ F304), I347 (≠ K345)
binding (2~{S},3~{S})-2-azanyl-3-[[3-[[4-(trifluoromethyl)phenyl]carbonylamino]phenyl]methoxy]butanedioic acid: I13 (= I9), M199 (= M197), S275 (= S273), T311 (≠ S309), G356 (≠ S354), L384 (= L376), D391 (= D383), R394 (= R386)

Sites not aligning to the query:

binding [(2~{R})-1-[2-azanylethoxy(oxidanyl)phosphoryl]oxy-3-hexadecanoyloxy-propan-2-yl] (~{Z})-octadec-9-enoate: 4

6r7rA Crystal structure of the glutamate transporter homologue glttk in complex with d-aspartate (see paper)
31% identity, 92% coverage: 9:407/435 of query aligns to 7:407/416 of 6r7rA

query
sites
6r7rA

I

I

V

L

A

W

G

G

M

L

A

V

L

L

G

G

V

A

I

V

V

F

G

G

Y

L

V

I

C

A

-

G

H
|
H

R

F

S

G

A

Y

A

A

G

G

A

A

A

V

E

K

A

T

K

-

T

-

I

-

A

-

G

-

Y

Y

F

I

S

K

I

P

I

F

T

G

D

D

I

L

F

F

L

V

R

R

L

L

V

L

K

K

M

M

I

L

I

V

A

M

P

P

L

I

V

V

F

L

A

A

T

S

L

L

V

V

S

V

G

G

L

A

A

A

G

S

M

I

E

S

G

-

T

P

S

A

D

R

V

L

R

G

R

R

I

V

G

G

F

V

R

K

S

I

V

V

G

V

W

Y

F

Y

V

L

C

A

A

T

S

S

L

A

F

M

S

A

L

V

A

F

L

F

G

G

L

L

A

I

L

V

A

G

N

R

A

L

L

F

Q

N

P

V

G

G

A

A

G

N

L

V

H

N

M

L

-

G

-

S

-

G

-

T

-

G

T

A

Q

Q

T

P

S

P

S

S

D

L

V

V

A

Q

T

T

G

L

L

L

N

N

T

I

A

V

G

-

L

-

N

-

F

-

K

-

D

-

F

-

V

-

T

-

H

-

A

-

F

-

P

P

S

T

S

N

I

P

I

F

D

A

A

S

M

L

A

A

R

K

N

G

D

E

I

V

L

L

Q

P

I

V

L

I

V

F

F

F

S

A

V

I

L

I

F

L

G

G

V

I

V

A

L

I

S

T

A

Y

I

L

-

M

-

N

K

R

K

N

D

E

P

E

R

R

V

V

-

R

-

K

-

S

-

A

T

E

P

T

L

L

I

L

A

R

G

V

I

F

D

D

A

G

L

L

V

A

P

E

A

A

M

M

L

Y

K

L

L

I

T

V

D

G

Y

G

V

V

M

M

R

Q

L

Y

A

A

P

P

I

I

G

G

V

V

F

F

G

A

A

L

L

I

A

A

S

Y

A

V

I

M

T

A

V

E

N

Q

G

G

L

V

D

R

V

V

L
x
V

T

G

T

P

Y

L

G
x
A

K

K

L

V

V

V

G

G

S

A

F

V

Y

Y

L

T

G

G

L

L

V

F

T

L

L

Q

W

I

I

V

V

I

L

T

I

Y

F

F

V

I

G

L

Y

L

A

K

F

V

L

F

G

G

K

I

S

D

I

P

W

I

R

K

L

F

L

I

K

R

A

K

V

A

R

K

E

D

P

A

A

M

M

I

L

T

A

A

F

F

S

V

T

T

A
x
R

S

S

S
|
S

E

S

A

G

A

T

Y

L

P

P

-

V

-

T

-

M

R

R

L

V

T

A

E

E

K

-

L

-

E

E

A

E

F

M

G

G

I

V

D

D

K

K

K

G

V

I

V

F

G

S

F

F

T

T

L

L

P

P

L

L

G

G

Y

A

A

T

F

I

N

N

L
x
M

D

D

G

G

S
x
T

M

A

M

L

Y

Y

Q

Q

A

G

F

V

A

T

A

V

I

L

F

F

I

V

A

A

Q

N

A

A

F

I

G

G

I

H

D

P

M

L

P

T

L

L

G

G

A

Q

Q

Q

I

L

M

V

L

V

L

L

V

T

L

A

M

V

L

L

S

A

S

S

K

I

G

G

M
x
T

A

A

G
|
G

V
|
V

A

P

R

G

G

A

S
x
G

V

A

V

I

V

M

V

L

A

A

A

M

I

V

A

L

P

Q

M

S

F

V

H

G

L

L

-

D

-

L

-

T

P

P

P

G

S

S

G

P

V

V

V

A

L

L

-

A

-

Y

-

A

-

M

I

I

L

L

A

G

I

I

D

D

Q

A

I

I

L

L

D
|
D

M

M

G

G

R
|
R

T
|
T

A

M

T

V

N
|
N

V

V

I

T

G

G

N

D

S

L

I

A

A

G

T

T

A

V

V

I

I

V

A

A

K

K

W

T

E

E

A

K

K

E

binding d-aspartic acid: R263 (≠ A271), S265 (= S273), M299 (≠ L306), T302 (≠ S309), T340 (≠ M347), G342 (= G349), V343 (= V350), G347 (≠ S354), D383 (= D383), R386 (= R386), T387 (= T387), N390 (= N390)
binding decyl-beta-d-maltopyranoside: H23 (= H24), V212 (≠ L220), A216 (≠ G224)

6zl4A The structure of glutamate transporter homologue glttk in complex with the photo switchable compound (cis) (see paper)
30% identity, 92% coverage: 9:407/435 of query aligns to 11:415/424 of 6zl4A

query
sites
6zl4A

I

I

V

L

A

W

G

G

M

L

A

V

L

L

G

G

V

A

I

V

V

F

G

G

Y

L

V

I

C

A

-

G

H

H

R

F

S

G

A

Y

A

A

G

G

A

A

A

V

E

K

A

T

K

-

T

-

I

-

A

-

G

-

Y

Y

F

I

S

K

I

P

I

F

T

G

D

D

I

L

F

F

L

V

R

R

L

L

V

L

K

K

M

M

I

L

I

V

A

M

P

P

L

I

V

V

F

L

A

A

T

S

L

L

V

V

S

V

G

G

L

A

A

A

G

S

M

I

E

S

G

-

T

P

S

A

D

R

V

L

R

G

R

R

I

V

G

G

F

V

R

K

S

I

V

V

G

V

W

Y

F

Y

V

L

C

A

A

T

S

S

L

A

F

M

S

A

L

V

A

F

L

F

G

G

L

L

A

I

L

V

A

G

N

R

A

L

L

F

Q

N

P

V

G

G

A

A

G

N

L

V

H

N

M

L

T

-

Q

-

T

-

S

G

S

S

D

G

V

T

A

G

T

K

G

A

L

I

N

E

T

A

A

Q

G

P

L

P

N

S

F

L

K

V

D

Q

F

T

V

L

T

L

H

N

A

I

F

V

P

P

S

T

S

N

I

P

I

F

D

A

A

S

M

L

A

A

R

K

N

G

D

E

I

V

L

L

Q

P

I

V

L

I

V

F

F

F

S

A

V

I

L

I

F

L

G

G

V

I

V

A

L

I

S

T

A

Y

I

L

-

M

-

N

K

R

K

N

D

E

P

E

R

R

V

V

-

R

-

K

-

S

-

A

T

E

P

T

L

L

I

L

A

R

G

V

I

F

D

D

A

G

L

L

V

A

P

E

A

A

M

M

L

Y

K
x
L

L

I

T

V

D

G

Y
x
G

V

V

M

M

R

Q

L

Y

A

A

P

P

I

I

G

G

V

V

F

F

G

A

A

L

L

I

A

A

S

Y

A

V

I

M

T

A

V

E

N

Q

G

G

L

V

D

R

V

V

L

V

T

G

T

P

Y

L

G

A

K

K

L

V

V

V

G

G

S

A

F

V

Y

Y

L

T

G

G

L

L

V

F

T

L

L

Q

W

I

I

V

V

I

L

T

I

Y

F

F

V

I

G

L

Y

L

A

K

F

V

L

F

G

G

K

I

S

D

I

P

W

I

R

K

L

F

L

I

K

R

A

K

V

A

R

K

E

D

P

A

A

M

M

I

L

T

A

A

F

F

S

V

T

T

A
x
R

S
|
S

E

S

A

G

A

T

Y

L

P

P

-

V

-

T

-

M

R
|
R

L

V

T

A

E

E

K

-

L

-

E

E

A

E

F

M

G

G

I

V

D

D

K

K

K

G

V

I

V

F

G

S

F

F

T

T

L

L

P

P

L

L

G

G

Y

A

A

T

F

I

N

N

L
x
M

D

D

G

G

S
x
T

M

A

M

L

Y

Y

Q

Q

A

G

F

V

A

T

A

V

I

L

F

F

I

V

A

A

Q

N

A

A

F

I

G

G

I

H

D

P

M

L

P

T

L

L

G

G

A

Q

Q

Q

I

L

M

V

L

V

L

L

V

T

L

A

M

V

L

L

S

A

S

S

K

I

G

G

M

T

A

A

G

G

V

V

A

P

R
x
G

G
x
A

S

G

V

A

V

I

V

M

V

L

A

A

A

M

I

V

A

L

P

Q

M

S

F

V

H

G

L

L

-

D

-

L

-

T

P

P

P

G

S

S

G

P

V

V

V

A

L

L

-

A

-

Y

-

A

-

M

I

I

L

L

A

G

I

I

D

D

Q

A

I

I

L

L

D

D

M

M

G

G

R
|
R

T
|
T

A

M

T

V

N

N

V

V

I

T

G

G

N

D

S

L

I

A

A

G

T

T

A

V

V

I

I

V

A

A

K

K

W

T

E

E

A

K

K

E

binding decyl-beta-d-maltopyranoside: L191 (≠ K191), G195 (≠ Y195), R282 (= R279)
binding (2~{S},3~{S})-2-azanyl-3-[[4-[2-(4-methoxyphenyl)hydrazinyl]phenyl]methoxy]butanedioic acid: R271 (≠ A271), S272 (= S272), S273 (= S273), M307 (≠ L306), T310 (≠ S309), G353 (≠ R352), A354 (≠ G353), R394 (= R386), T395 (= T387)

Sites not aligning to the query:

binding decyl-beta-d-maltopyranoside: 3

6zgbA Glutamate transporter homologue glttk in complex with a photo cage compound (see paper)
30% identity, 92% coverage: 9:407/435 of query aligns to 12:416/425 of 6zgbA

query
sites
6zgbA

I

I

V

L

A

W

G

G

M

L

A

V

L

L

G

G

V

A

I

V

V

F

G

G

Y

L

V

I

C

A

-

G

H

H

R

F

S

G

A

Y

A

A

G

G

A

A

A

V

E

K

A

T

K

-

T

-

I

-

A

-

G

-

Y

Y

F

I

S

K

I

P

I

F

T

G

D

D

I

L

F

F

L

V

R

R

L

L

V

L

K

K

M

M

I

L

I

V

A

M

P

P

L

I

V

V

F

L

A

A

T

S

L

L

V

V

S

V

G

G

L

A

A

A

G

S

M

I

E

S

G

-

T

P

S

A

D

R

V

L

R

G

R

R

I

V

G

G

F

V

R

K

S

I

V

V

G

V

W

Y

F

Y

V

L

C

A

A

T

S

S

L

A

F

M

S

A

L

V

A

F

L

F

G

G

L

L

A

I

L

V

A

G

N

R

A

L

L

F

Q

N

P

V

G

G

A

A

G

N

L

V

H

N

M

L

T

-

Q

-

T

-

S

G

S

S

D

G

V

T

A

G

T

K

G

A

L

I

N

E

T

A

A

Q

G

P

L

P

N

S

F

L

K

V

D

Q

F

T

V

L

T

L

H

N

A

I

F

V

P

P

S

T

S

N

I

P

I

F

D

A

A

S

M

L

A

A

R

K

N

G

D

E

I

V

L

L

Q

P

I

V

L

I

V

F

F

F

S

A

V

I

L

I

F

L

G

G

V

I

V

A

L

I

S

T

A

Y

I

L

-

M

-

N

K

R

K

N

D

E

P

E

R

R

V

V

-

R

-

K

-

S

-

A

T

E

P

T

L

L

I

L

A

R

G

V

I

F

D

D

A

G

L

L

V

A

P

E

A

A

M

M

L

Y

K

L

L

I

T

V

D

G

Y

G

V

V

M

M

R

Q

L

Y

A

A

P

P

I

I

G

G

V

V

F

F

G

A

A

L

L

I

A

A

S

Y

A

V

I

M

T

A

V

E

N

Q

G

G

L

V

D

R

V

V

L

V

T

G

T

P

Y

L

G

A

K

K

L

V

V

V

G

G

S

A

F

V

Y

Y

L

T

G

G

L

L

V

F

T

L

L

Q

W

I

I

V

V

I

L

T

I

Y

F

F

V

I

G

L

Y

L

A

K

F

V

L

F

G

G

K

I

S

D

I

P

W

I

R

K

L

F

L

I

K

R

A

K

V

A

R

K

E

D

P

A

A

M

M

I

L

T

A

A

F

F

S

V

T

T

A
x
R

S
|
S

E

S

A

G

A

T

Y

L

P

P

-

V

-

T

-

M

R

R

L

V

T

A

E

E

K

-

L

-

E

E

A

E

F

M

G

G

I

V

D

D

K

K

K

G

V

I

V

F

G

S

F

F

T

T

L

L

P

P

L

L

G

G

Y

A

A

T

F

I

N

N

L
x
M

D

D

G

G

S

T

M

A

M

L

Y

Y

Q

Q

A

G

F

V

A

T

A

V

I

L

F

F

I

V

A

A

Q

N

A

A

F

I

G

G

I

H

D

P

M

L

P

T

L

L

G

G

A

Q

Q

Q

I

L

M

V

L

V

L

L

V

T

L

A

M

V

L

L

S

A

S

S

K

I

G

G

M

T

A

A

G

G

V

V

A

P

R

G

G

A

S

G

V

A

V

I

V

M

V

L

A

A

A

M

I

V

A

L

P

Q

M

S

F

V

H

G

L

L

-

D

-

L

-

T

P

P

P

G

S

S

G

P

V

V

V

A

L

L

-

A

-

Y

-

A

-

M

I

I

L

L

A

G

I

I

D

D

Q

A

I

I

L

L

D
|
D

M

M

G

G

R
|
R

T
|
T

A

M

T

V

N

N

V

V

I

T

G

G

N

D

S

L

I

A

A

G

T

T

A

V

V

I

I

V

A

A

K

K

W

T

E

E

A

K

K

E

binding (2~{S},3~{S})-2-azanyl-3-[(2-nitrophenyl)methoxy]butanedioic acid: R272 (≠ A271), S273 (= S272), S274 (= S273), M308 (≠ L306), D392 (= D383), R395 (= R386), T396 (= T387)

6xwnB Structure of glutamate transporter homologue glttk in the presence of tboa inhibitor (see paper)
30% identity, 92% coverage: 9:407/435 of query aligns to 14:418/426 of 6xwnB

query
sites
6xwnB

I

I

V

L

A

W

G

G

M

L

A

V

L

L

G

G

V

A

I

V

V

F

G

G

Y

L

V

I

C

A

-

G

H

H

R

F

S

G

A

Y

A

A

G

G

A

A

A

V

E

K

A

T

K

-

T

-

I

-

A

-

G

-

Y

Y

F

I

S

K

I

P

I

F

T

G

D

D

I

L

F

F

L

V

R

R

L

L

V

L

K

K

M

M

I

L

I

V

A

M

P

P

L

I

V

V

F

L

A

A

T

S

L

L

V

V

S

V

G

G

L

A

A

A

G

S

M

I

E

S

G

-

T

P

S

A

D

R

V

L

R

G

R

R

I

V

G

G

F

V

R

K

S

I

V

V

G

V

W

Y

F

Y

V

L

C

A

A

T

S

S

L

A

F

M

S

A

L

V

A

F

L

F

G

G

L

L

A

I

L

V

A

G

N

R

A

L

L

F

Q

N

P

V

G

G

A

A

G

N

L

V

H

N

M

L

T

-

Q

-

T

-

S

G

S

S

D

G

V

T

A

G

T

K

G

A

L

I

N

E

T

A

A

Q

G

P

L

P

N

S

F

L

K

V

D

Q

F

T

V

L

T

L

H

N

A

I

F

V

P

P

S

T

S

N

I

P

I

F

D

A

A

S

M

L

A

A

R

K

N

G

D

E

I

V

L

L

Q

P

I

V

L

I

V

F

F

F

S

A

V

I

L

I

F

L

G

G

V

I

V

A

L

I

S

T

A

Y

I

L

-

M

-

N

K

R

K

N

D

E

P

E

R

R

V

V

-

R

-

K

-

S

-

A

T

E

P

T

L

L

I

L

A

R

G

V

I

F

D

D

A

G

L

L

V

A

P

E

A

A

M

M

L

Y

K

L

L

I

T

V

D

G

Y

G

V

V

M

M

R

Q

L

Y

A

A

P

P

I

I

G

G

V

V

F

F

G

A

A

L

L

I

A

A

S

Y

A

V

I

M

T

A

V

E

N

Q

G

G

L

V

D

R

V

V

L

V

T

G

T

P

Y

L

G

A

K

K

L

V

V

V

G

G

S

A

F

V

Y

Y

L

T

G

G

L

L

V

F

T

L

L

Q

W

I

I

V

V

I

L

T

I

Y

F

F

V

I

G

L

Y

L

A

K

F

V

L

F

G

G

K

I

S

D

I

P

W

I

R

K

L

F

L

I

K

R

A

K

V

A

R

K

E

D

P

A

A

M

M

I

L

T

A

A

F

F

S

V

T

T

A

R

S
|
S

E

S

A

G

A

T

Y

L

P

P

-

V

-

T

-

M

R

R

L

V

T

A

E

E

K

-

L

-

E

E

A

E

F

M

G

G

I

V

D

D

K

K

K

G

V

I

V

F

G

S

F

F

T

T

L

L

P

P

L

L

G

G

Y

A

A

T

F

I

N

N

L
x
M

D

D

G

G

S
x
T

M

A

M

L

Y

Y

Q

Q

A

G

F

V

A

T

A

V

I

L

F

F

I

V

A

A

Q

N

A

A

F

I

G

G

I

H

D

P

M

L

P

T

L

L

G

G

A

Q

Q

Q

I

L

M

V

L

V

L

L

V

T

L

A

M

V

L

L

S

A

S

S

K

I

G

G

M

T

A

A

G

G

V

V

A

P

R

G

G

A

S

G

V

A

V

I

V

M

V

L

A

A

A

M

I

V

A

L

P

Q

M

S

F

V

H

G

L

L

-

D

-

L

-

T

P

P

P

G

S

S

G

P

V

V

V

A

L

L

-

A

-

Y

-

A

-

M

I

I

L

L

A

G

I

I

D

D

Q

A

I

I

L

L

D
|
D

M

M

G

G

R
|
R

T

T

A

M

T

V

N

N

V

V

I

T

G

G

N

D

S

L

I

A

A

G

T

T

A

V

V

I

I

V

A

A

K

K

W

T

E

E

A

K

K

E

binding (3s)-3-(benzyloxy)-l-aspartic acid: S275 (= S272), S276 (= S273), M310 (≠ L306), T313 (≠ S309), D394 (= D383), R397 (= R386)

5e9sA Crystal structure of substrate-bound glutamate transporter homologue glttk (see paper)
30% identity, 92% coverage: 9:407/435 of query aligns to 14:418/427 of 5e9sA

query
sites
5e9sA

I

I

V

L

A

W

G

G

M

L

A

V

L

L

G

G

V

A

I

V

V

F

G

G

Y

L

V

I

C

A

-

G

H

H

R

F

S

G

A

Y

A

A

G

G

A

A

A

V

E

K

A

T

K

-

T

-

I

-

A

-

G

-

Y

Y

F

I

S

K

I

P

I

F

T

G

D

D

I

L

F

F

L

V

R

R

L

L

V

L

K

K

M

M

I

L

I

V

A

M

P

P

L

I

V

V

F

L

A

A

T

S

L

L

V

V

S

V

G

G

L

A

A

A

G

S

M

I

E

S

G

-

T

P

S

A

D

R

V

L

R

G

R

R

I

V

G

G

F

V

R

K

S

I

V

V

G

V

W

Y

F
x
Y

V

L

C

A

A
x
T

S
|
S

L

A

F

M

S

A

L

V

A

F

L

F

G

G

L

L

A

I

L

V

A

G

N

R

A

L

L

F

Q

N

P

V

G

G

A

A

G

N

L

V

H

N

M

L

T

-

Q

-

T

-

S

G

S

S

D

G

V

T

A

G

T

K

G

A

L

I

N

E

T

A

A

Q

G

P

L

P

N

S

F

L

K

V

D

Q

F

T

V

L

T

L

H

N

A

I

F

V

P

P

S

T

S

N

I

P

I

F

D

A

A

S

M

L

A

A

R

K

N

G

D

E

I

V

L

L

Q

P

I

V

L

I

V

F

F

F

S

A

V

I

L

I

F

L

G

G

V

I

V

A

L

I

S

T

A

Y

I

L

-

M

-

N

K

R

K

N

D

E

P

E

R

R

V

V

-

R

-

K

-

S

-

A

T

E

P

T

L

L

I

L

A

R

G

V

I

F

D

D

A

G

L

L

V

A

P

E

A

A

M

M

L

Y

K
x
L

L

I

T

V

D

G

Y
x
G

V

V

M

M

R

Q

L
x
Y

A

A

P

P

I

I

G

G

V

V

F

F

G

A

A

L

L

I

A

A

S

Y

A

V

I

M

T

A

V

E

N

Q

G

G

L

V

D

R

V

V

L

V

T

G

T

P

Y

L

G

A

K

K

L

V

V

V

G

G

S

A

F

V

Y

Y

L

T

G

G

L

L

V

F

T

L

L

Q

W

I

I

V

V

I

L

T

I

Y

F

F

V

I

G

L

Y

L

A

K

F

V

L

F

G

G

K

I

S

D

I

P

W

I

R

K

L

F

L

I

K

R

A

K

V

A

R

K

E

D

P

A

A

M

M

I

L

T

A

A

F

F

S

V

T

T

A
x
R

S
|
S

E

S

A

G

A

T

Y

L

P

P

-

V

-

T

-

M

R

R

L

V

T

A

E

E

K

-

L

-

E

E

A

E

F

M

G

G

I

V

D

D

K

K

K

G

V

I

V

F

G

S

F

F

T

T

L

L

P

P

L

L

G
|
G

Y
x
A

A
x
T

F

I

N
|
N

L
x
M

D
|
D

G

G

S
x
T

M

A

M

L

Y

Y

Q

Q

A

G

F

V

A

T

A

V

I

L

F

F

I

V

A

A

Q

N

A

A

F

I

G

G

I

H

D

P

M

L

P

T

L

L

G

G

A

Q

Q

Q

I

L

M

V

L

V

L

L

V

T

L

A

M

V

L

L

S

A

S
|
S

K
x
I

G
|
G

M
x
T

A

A

G
|
G

V
|
V

A

P

R

G

G
x
A

S
x
G

V

A

V

I

V

M

V

L

A

A

A

M

I

V

A

L

P

Q

M

S

F

V

H

G

L

L

-

D

-

L

-

T

P

P

P

G

S

S

G

P

V

V

V

A

L

L

-

A

-

Y

-

A

-

M

I

I

L

L

A

G

I

I

D

D

Q

A

I

I

L

L

D
|
D

M

M

G

G

R
|
R

T
|
T

A

M

T

V

N
|
N

V
|
V

I

T

G

G

N
x
D

S

L

I

A

A

G

T

T

A

V

V

I

I

V

A

A

K

K

W

T

E

E

A

K

K

E

binding aspartic acid: R274 (≠ A271), S275 (= S272), S276 (= S273), T313 (≠ S309), G353 (= G349), V354 (= V350), A357 (≠ G353), G358 (≠ S354), D394 (= D383), R397 (= R386), T398 (= T387)
binding decyl-beta-d-maltopyranoside: L194 (≠ K191), G198 (≠ Y195), Y202 (≠ L199)
binding sodium ion: Y87 (≠ F87), T90 (≠ A90), S91 (= S91), S276 (= S273), G305 (= G301), A306 (≠ Y302), T307 (≠ A303), N309 (= N305), N309 (= N305), M310 (≠ L306), D311 (= D307), S348 (= S344), I349 (≠ K345), G350 (= G346), T351 (≠ M347), N401 (= N390), V402 (= V391), D405 (≠ N394)

6bmiA Crystal structure of gltph r397c in complex with l-serine (see paper)
30% identity, 91% coverage: 9:405/435 of query aligns to 8:396/396 of 6bmiA

query
sites
6bmiA

I

I

V

L

A

I

G

G

M

L

A

I

L

L

G

G

V

A

I

I

V

V

G

G

Y

L

V

I

C

L

-

G

H

H

R

Y

S

G

A

Y

A

A

G

D

A

A

A

V

E

K

A

T

K

-

T

-

I

-

A

-

G

-

Y

Y

F

V

S

K

I

P

I

F

T

G

D

D

I

L

F

F

L

V

R

R

L

L

V

L

K

K

M

M

I

L

I

V

A

M

P

P

L

I

V

V

F

F

A

A

T

S

L

L

V

V

S

V

G

G

L

A

A

A

G

S

M

I

E

S

G

-

T

P

S

A

D

R

V

L

R

G

R

R

I

V

G

G

F

V

R

K

S

I

V

V

G

V

W

Y

F

Y

V

L

C

L

A

T

S

S

L

A

F

F

S

A

L

V

A

T

L

L

G

G

L

I

A

I

L

M

A

A

N

R

A

L

L

F

Q

N

P

P

G

G

A

A

G

G

L

I

H

H

M

-

T

-

Q

-

T

-

S

-

D

-

V

-

A

-

T

-

G

-

L

L

N

A

T

V

A

G

G

G

L

Q

N

Q

F

F

K

Q

-

P

-

K

-

Q

-

A

-

P

-

L

-

V

D

K

F

I

V

L

T

L

H

D

A

I

F

V

P

P

S

T

S

N

I

P

I

F

D

G

A

A

M

L

A

A

R

N

N

G

D

Q

I

V

L

L

Q

P

I

T

L

I

V

F

F

F

S

A

V

I

L

I

F

L

G

G

V

I

V

A

L

I

S

T

A

Y

I

L

-

M

-

N

-

S

-

E

-

N

-

E

K

K

K

V

D

R

P

K

R

S

V

A

T

E

P

T

L

L

I

L

A

D

G

A

I

I

D

N

A

G

L

L

V

A

P

E

A

A

M

M

L

Y

K

K

L

I

T

V

D

N

Y

G

V

V

M

M

R

Q

L

Y

A

A

P

P

I

I

G

G

V

V

F

F

G

A

A

L

L

I

A

A

S

Y

A

V

I

M

T

A

V

E

N

Q

G

G

L

V

D

K

V

V

L

V

T

G

T

E

Y

L

G

A

K

K

L

V

V

T

G

A

S

A

F

V

Y

Y

L

V

G

G

L

L

V

-

T

T

L

L

W

Q

I

I

V

L

L

L

I

V

-

Y

F

F

V

V

G

L

Y

L

A

K

F

I

L

Y

G

G

K

I

S

D

I

P

W

I

R

S

L

F

L

I

K

K

A

K

V

A

R

K

E

D

P

A

A

M

M

L

L

T

A

A

F

F

S

V

T

T

A
x
R

S

S

S
|
S

E

S

A

G

A

T

Y

L

P

P

R

V

L

T

T

M

E

R

K

V

L

A

E

K

A

E

F

M

G

G

I

I

D

S

K

E

K

G

V

I

V

Y

G

S

F

F

T

T

L

L

P

P

L

L

G

G

Y

A

A

T

F

I

N

N

L

M

D

D

G

G

S
x
T

M

A

M

L

Y

Y

Q

Q

A

G

F

V

A

C

A

T

I

F

F

F

I

I

A

A

Q

N

A

A

F

L

G

G

I

S

D

H

M

L

P

T

L

V

G

G

A

Q

Q

Q

I

L

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T

M

I

L

V

L

L

V

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L

A

M

V

L

L

S

A

S

-

K

-

G

-

M

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A

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G

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A

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R

-

G

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-

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-

V

I

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L

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A

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Y

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A

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M

I

I

L

L

A

G

I

I

D

D

Q

A

I

I

L

L

D
|
D

M

M

G

G

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C

T
|
T

A

M

T

V

N
|
N

V

V

I

T

G

G

N

D

S

L

I

T

A

G

T

T

A

A

V

I

I

V

A

A

K

K

W

T

E

E

binding serine: R268 (≠ A271), S270 (= S273), T306 (≠ S309), D374 (= D383), T378 (= T387), N381 (= N390)

O35874 Neutral amino acid transporter A; Alanine/serine/cysteine/threonine transporter 1; ASCT-1; Solute carrier family 1 member 4 from Mus musculus (Mouse) (see 2 papers)
30% identity, 96% coverage: 16:433/435 of query aligns to 51:495/532 of O35874

query
sites
O35874

G

G

V

V

I

L

V

V

G

G

Y

A

V

G

C

M

H

G

R

A

S

A

A

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A

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G

G

A

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K

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Y

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M

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P

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L

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V

F

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L

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V

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S

G

G

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A

A

A

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A

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M

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N
|
N

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N

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D

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N

V

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G

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I

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K

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W

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A

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Q

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A

V

V

V

K

K

H

K

I

G

D

E

G

-

A

-

D

-

S

-

P

-

A

-

L

-

G

-

R

-

L

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Q

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E

L

L

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Q

E

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L

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Q

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G

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E

E

N201 (= N150) modified: carbohydrate, N-linked (GlcNAc...) asparagine
N206 (vs. gap) modified: carbohydrate, N-linked (GlcNAc...) asparagine

7awmA Structure of the thermostabilized eaat1 cryst mutant in complex with l-asp, three sodium ions and the allosteric inhibitor ucph101 (see paper)
27% identity, 91% coverage: 16:412/435 of query aligns to 33:409/412 of 7awmA

query
sites
7awmA

G

G

V

V

I

V

V

L

G

G

Y

F

V

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C

L

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R

R

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Y

A

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A

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A

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A

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K

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I

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A

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Y

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K

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P

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G

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L

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A

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L

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D

G

A

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A

D
x
S

V

-

R
x
G

R

R

I

L

G
|
G

F

M

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x
A

V
|
V

G

V

W

Y

F
x
Y

V

M

C

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A

T

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I

F

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A

L

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A

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L

L

G

G

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A

I

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L

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N

L

A

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H

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P

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G

-

L

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H

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M

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Q

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D

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A

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L

L

N

D

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A

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G

-

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N

-

F

F

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L

D

D

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N

A

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F

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P

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I

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A

M

A

A

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R

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-

Y

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E

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G

-

M

D

N

I

I

L

L

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I

L

L

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V

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F

F

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x
M

L

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G

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x
F

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A

L

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A

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I

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K

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P

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A

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G

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|
S

E

S

A

A

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P

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K

C

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L

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-

N

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G

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D

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K

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|
F

T

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x
V

G

G

Y

A

A

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F

I

N

N

L

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D

D

G

G

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x
T

M

A

M

L

Y

Y

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A

A

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A

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I

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F

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N

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S

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|
G

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x
I

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x
A

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x
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A

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G

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I

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L

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D
|
D

M

R

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F

R
|
R

T
|
T

A

M

T

V

N

N

V

V

I

L

G

G

N

D

S

A

I

L

A

G

T

A

A

G

V

I

I

V

A

E

K

H

W

L

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K

K

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A

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H

Q

binding 2-Amino-5,6,7,8-tetrahydro-4-(4-methoxyphenyl)-7-(naphthalen-1-yl)-5-oxo-4H-chromene-3-carbonitrile: S88 (≠ D75), G89 (≠ R77), G92 (= G80), A95 (≠ S83), V96 (= V84), Y99 (≠ F87), M163 (≠ V160), F167 (≠ V164), F293 (= F296), V297 (≠ L300)
binding aspartic acid: S268 (= S272), S269 (= S273), T306 (≠ S309), G346 (= G349), I347 (≠ V350), A350 (≠ G353), G351 (≠ S354), D380 (= D383), R383 (= R386), T384 (= T387)

P43007 Neutral amino acid transporter A; Alanine/serine/cysteine/threonine transporter 1; ASCT-1; Solute carrier family 1 member 4 from Homo sapiens (Human) (see 4 papers)
30% identity, 87% coverage: 33:411/435 of query aligns to 68:484/532 of P43007

query
sites
P43007

A

S

K

R

T

T

I

Q

A

V

G

T

Y

Y

F

L

S

A

I

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T

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D

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I

M

F

L

L

L

R

R

L

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L

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M

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I

A

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P

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L

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V

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V

A

C

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S

L

L

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V

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G

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A

A

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R

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G

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G

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F

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C

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A

A

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A

N

F

A

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K

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P

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G

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G

G

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A

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M

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Q

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S

S

D

D

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L

A

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G

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F

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A

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F

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P

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N

I

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A

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-

Y

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A

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N

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P

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K

K

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N

-

N

G

G

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D

D

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K

R

V

I

V

S

G

R

F

F

T

I

L

L

P

P

L

I

G

G

Y

A

A

T

F

V

N

N

L

M

D

D

G

G

S

A

M

A

M

I

Y

F

Q

Q

A

C

F

V

A

A

I

V

F

F

I

I

A

A

Q

Q

A

L

F

N

G

N

I

V

D

E

M

L

P

N

L

A

G

G

A

Q

Q

I

I

F

M

T

M

I

L

L

L

V

V

T

L

A

M

T

L

A

S

S

K

V

G

G

M

A

A

A

G

G

V

V

A

P

R

A

G

G

S

G

V

V

V

L

V

T

V

I

A

A

A

I

I

I

A

L

P

E

M

A

F

I

H

G

L

L

P

P

P

T

S

H

G

D

V

L

V

P

L

L

I

I

L

L

A

A

I

V

D

D

Q

W

I

I

L

V

D

D

M
x
R

G

T

R

T

T

T

A

V

T

V

N

N

V

V

I

E

G

G

N

D

S

A

I

L

A

G

T

A

A

G

V

I

I

L

A

H

K

H

W

L

E

N

A

Q

K

K

R

A

A

T

V

K

K

K

N201 (vs. gap) modified: carbohydrate, N-linked (GlcNAc...) asparagine
N206 (vs. gap) modified: carbohydrate, N-linked (GlcNAc...) asparagine
E256 (≠ P187) to K: in SPATCCM; does not affect localization at the cell surface; decreased uptake of L-serine and L-alanine; Vmax is decreased by at least 50% for both substrates; 3-fold increase of affinity for L-serine; 2-fold increase of affinity for L-alanine; dbSNP:rs201278558
R457 (≠ M384) to W: in SPATCCM; does not affect localization at the cell surface; loss of uptake of L-serine and L-alanine; dbSNP:rs761533681

6x2zA Heaat3-ofs-asp (see paper)
26% identity, 95% coverage: 2:414/435 of query aligns to 2:418/419 of 6x2zA

query
sites
6x2zA

K

N

N

N

R

W

L

V

T

L

F

L

Y

S

I

T

V

V

A

A

G

A

M

V

A

V

L

L

G

G

V

I

I

T

V

T

G

G

Y

V

V

L

C

V

H

R

R

E

S

H

A

S

A

N

G

L

A

S

A

T

E

L

A

E

K

K

T

F

I

-

A

-

G

-

Y

Y

F

F

S

A

I

F

I

P

T

G

D

E

I

I

F

L

L

M

R

R

L

M

V

L

K

K

M

L

I

I

A

L

P

P

L

L

V

I

F

I

A

S

T

S

L

M

V

I

S

T

G

G

L

V

A

A

G

A

M

L

E

D

G

-

T

-

S

S

D

N

V

V

R

S

-

G

R

K

I

I

G

G

F

L

R

R

S

A

V

V

G

V

W

Y

F
x
Y

V

F

C

C

A
x
T

S
x
T

L

L

F

I

S

A

L

V

A

I

L

L

G

G

L

I

A

V

L

L

A

V

N

V

A

S

L

I

Q

K

P

P

G

G

A

-

G

-

L

-

H

-

M

V

T

T

Q

Q

T

K

S

V

S

G

D

S

V

T

A

V

T

D

G

A

L

M

N

-

T

-

A

-

G

-

L

-

N

-

F

-

K

L

D

D

F

L

V

I

T

R

H

N

A

M

F

F

P

P

S

E

S

N

I

L

I

V

D

Q

A

A

M

C

A

F

R

Q

N

Q

-

Y

-

K

-

T

-

K

-

R

-

E

-

Y

-

K

-

I

-

V

-

G

-

M

-

Y

-

S

-

D

-

G

-

I

D

N

I

V

L

L

Q

G

I

L

L

I

V

V

F

F

S

C

V

L

L

V

F

F

G

G

V

L

V

V

L

I

S

G

A

K

I

M

K

G

K

E

D

K

P

G

R

Q

V

I

T

-

P

-

L

L

I

V

A

D

G

F

I

F

D

N

A

A

L

L

V

S

P

D

A

A

M

T

L

M

K

K

L

I

T

V

D

Q

Y

I

V

I

M

M

R

C

L

Y

A

M

P

P

I

L

G

G

V

I

F

L

G

F

A

L

L

I

A

A

S

G

A

K

I

I

-

I

T

E

V

V

N

E

G

D

L

W

D

E

V

I

L

F

T

R

T

K

Y

L

G

G

K

L

L

Y

V

M

G

A

S

T

F

V

Y

L

L

T

G

G

L

L

V

A

T

I

L

H

W

S

I

I

V

V

L

I

I

L

-

P

F

L

V

I

G

Y

Y

F

A

I

F

V

L

V

G

R

K

K

S

N

I

P

W

F

R

R

L

F

L

A

K

M

A

G

V

M

R

A

E

Q

P

A

A

L

M

L

L

T

A

A

F

L

S

M

T

I

A
x
S

S

S

S
|
S

E

S

A

A

A

T

Y

L

P

P

-

V

-

T

-

F

R

R

L

C

T

A

E

E

K

-

L

-

E

E

A

N

F

N

G

Q

I

V

D

D

K

K

K

R

V

I

V

T

G

R

F

F

T

V

L

L

P

P

L

V

G
|
G

Y
x
A

A
x
T

F
x
I

N
|
N

L
x
M

D
|
D

G

G

S
x
T

M

A

M

L

Y

Y

Q

E

A

A

F

V

A

A

I

V

F

F

I

I

A

A

Q

Q

A

L

F

N

G

D

I

L

D

D

M

L

P

G

L

I

G

G

A

Q

Q

I

I

I

M

T

M

I

L

S

L

I

V

T

L

A

M

T

L

S

S

A

S
|
S

K
x
I

G
|
G

M
x
A

A

A

G
|
G

V
|
V

A

P

R

Q

G

A

S

G

V

L

V

V

V

T

V

M

A

V

A

I

I

V

A

L

P

S

M

A

F

V

H

G

L

L

P

P

P

A

S

E

G

D

V

V

V

T

L

L

I

I

L

I

A

A

I

V

D

D

Q

W

I

L

L

L

D
|
D

M

R

G

F

R
|
R

T
|
T

A

M

T

V

N
|
N

V

V

I

L

G

G

N
x
D

S

A

I

F

A

G

T

T

A

G

V

I

I

V

A

E

K

K

W

L

E

-

A

S

K

K

R

K

A

E

V

L

K

E

H

Q

I

M

D

D

binding aspartic acid: S275 (≠ A271), S277 (= S273), T314 (≠ S309), G354 (= G349), V355 (= V350), D388 (= D383), R391 (= R386), T392 (= T387)
binding sodium ion: Y83 (≠ F87), T86 (≠ A90), T87 (≠ S91), G306 (= G301), A307 (≠ Y302), T308 (≠ A303), I309 (≠ F304), N310 (= N305), N310 (= N305), M311 (≠ L306), M311 (≠ L306), D312 (= D307), S349 (= S344), I350 (≠ K345), G351 (= G346), A352 (≠ M347), N395 (= N390), D399 (≠ N394)

8cv2A Human excitatory amino acid transporter 3 (eaat3) in an outward facing sodium-bound state (see paper)
26% identity, 95% coverage: 2:414/435 of query aligns to 4:431/433 of 8cv2A

query
sites
8cv2A

K

N

N

N

R

W

L

V

T

L

F

L

Y

S

I

T

V

V

A

A

G

A

M

V

A

V

L

L

G

G

V

I

I

T

V

T

G

G

Y

V

V

L

C

V

H

R

R

E

S

H

A

S

A

N

G

L

A

S

A

T

E

L

A

E

K

K

T

F

I

-

A

-

G

-

Y

Y

F

F

S

A

I

F

I

P

T

G

D

E

I

I

F

L

L

M

R

R

L

M

V

L

K

K

M

L

I

I

A

L

P

P

L

L

V

I

F

I

A

S

T

S

L

M

V

I

S

T

G

G

L

V

A

A

G

A

M

L

E

D

G

-

T

-

S

S

D

N

V

V

R

S

-

G

R

K

I

I

G

G

F

L

R

R

S

A

V

V

G

V

W

Y

F
x
Y

V

F

C

A

A
x
T

S
x
T

L

L

F

I

S

A

L

V

A

I

L

L

G

G

L

I

A

V

L

L

A

V

N

V

A

S

L

I

Q

K

P

P

G

G

A

-

G

-

L

-

H

-

M

V

T

T

Q

Q

T

K

S

V

S

G

D

E

V

I

A

A

T

R

G

T

L

G

N

S

T

T

A

P

G

E

L

V

N

S

-

T

-

V

-

D

-

A

F

M

K

L

D

D

F

L

V

I

T

R

H

N

A

M

F

F

P

P

S

E

S

N

I

L

I

V

D

Q

A

A

M

A

A

F

R

Q

N

Q

-

Y

-

K

-

T

-

K

-

R

-

E

-

Y

-

K

-

I

-

V

-

G

-

M

-

Y

-

S

-

D

-

G

-

I

D

N

I

V

L

L

Q

G

I

L

L

I

V

V

F

F

S

A

V

L

L

V

F

F

G

G

V

L

V

V

L

I

S

G

A

K

I

M

K

G

K

E

D

K

P

G

R

Q

V

I

T

-

P

-

L

L

I

V

A

D

G

F

I

F

D

N

A

A

L

L

V

S

P

D

A

A

M

T

L

M

K

K

L

I

T

V

D

Q

Y

I

V

I

M

M

R

W

L

Y

A

M

P

P

I

L

G

G

V

I

F

L

G

F

A

L

L

I

A

A

S

G

A

C

I

I

-

I

T

E

V

V

N

E

G

D

L

W

D

E

V

I

L

F

T

R

T

K

Y

L

G

G

K

L

L

Y

V

M

G

A

S

T

F

V

Y

L

L

T

G

G

L

L

V

A

T

I

L

H

W

S

I

I

V

V

L

I

I

L

-

P

F

L

V

I

G

Y

Y

F

A

I

F

V

L

V

G

R

K

K

S

N

I

P

W

F

R

R

L

F

L

A

K

M

A

G

V

M

R

A

E

Q

P

A

A

L

M

L

L

T

A

A

F

L

S

M

T

I

A

S

S

S

E

S

A

A

A

T

Y

L

P

P

-

V

-

T

-

F

R

R

L

C

T

A

E

E

K

-

L

-

E

E

A

N

F

N

G

Q

I

V

D

D

K

K

K

R

V

I

V

T

G

R

F

F

T

V

L

L

P

P

L

V

G
|
G

Y
x
A

A

T

F

I

N
|
N

L
x
M

D
|
D

G

G

S

T

M

A

M

L

Y

Y

Q

E

A

A

F

V

A

A

I

V

F

F

I

I

A

A

Q

Q

A

L

F

N

G

D

I

L

D

D

M

L

P

G

L

I

G

G

A

Q

Q

I

I

I

M

T

M

I

L

S

L

I

V

T

L

A

M

T

L

S

S

A

S

S

K

I

G

G

M

A

A

A

G

G

V

V

A

P

R

Q

G

A

S

G

V

L

V

V

V

T

V

M

A

V

A

I

I

V

A

L

P

S

M

A

F

V

H

G

L

L

P

P

P

A

S

E

G

D

V

V

V

T

L

L

I

I

L

I

A

A

I

V

D

D

Q

C

I

L

L

L

D

D

M

R

G

F

R

R

T

T

A

M

T

V

N
|
N

V

V

I

L

G

G

N
x
D

S

A

I

F

A

G

T

T

A

G

V

I

I

V

A

E

K

K

W

L

E

-

A

S

K

K

R

K

A

E

V

L

K

E

H

Q

I

M

D

D

binding sodium ion: Y85 (≠ F87), T88 (≠ A90), T89 (≠ S91), G319 (= G301), A320 (≠ Y302), N323 (= N305), N323 (= N305), M324 (≠ L306), D325 (= D307), N408 (= N390), D412 (≠ N394)

7nsgA Structure of human excitatory amino acid transporter 3 (eaat3) in complex with hip-b
26% identity, 95% coverage: 2:414/435 of query aligns to 8:428/430 of 7nsgA

query
sites
7nsgA

K

N

N

N

R

W

L

V

T

L

F

L

Y

S

I

T

V

V

A

A

G

A

M

V

A

V

L

L

G

G

V

I

I

T

V

T

G

G

Y

V

V

L

C

V

-

R

-

E

H

H

R

R

S

N

A

L

A

S

G

T

A

L

A

E

E

K

A

F

K

-

T

-

I

-

A

-

G

-

Y

Y

F

F

S

A

I

F

I

P

T

G

D

E

I

I

F

L

L

M

R

R

L

M

V

L

K

K

M

L

I

I

A

L

P

P

L

L

V

I

F

I

A

S

T

S

L

M

V

I

S

T

G

G

L

V

A

A

G

A

M

L

E

D

G

-

T

-

S

S

D

N

V

V

R

S

-

G

R

K

I

I

G

G

F

V

R

R

S

A

V

V

G

V

W

Y

F

Y

V

F

C

C

A

T

S

T

L

L

F

I

S

A

L

V

A

I

L

L

G

G

L

I

A

V

L

L

A

V

N

V

A

S

L

I

Q

K

P

P

G

G

A

-

G

-

L

-

H

-

M

V

T

T

Q

Q

T

V

S

S

S

T

D

V

V

D

A

A

T

-

G

-

L

-

N

-

T

-

A

-

G

-

L

-

N

-

F

M

K

L

D

D

F

L

V

I

T

R

H

N

A

M

F

F

P

P

S

E

S

N

I

L

I

V

D

Q

A

A

M

C

A

F

R

Q

N

Q

-

Y

-

K

-

T

-

K

-

R

-

E

-

V

-

K

-

P

-

K

-

T

-

K

-

E

-

Y

-

K

-

I

-

V

-

G

-

M

-

Y

-

S

-

D

-

G

-

I

D

N

I

V

L

L

Q

G

I

L

L

I

V

V

F

F

S

C

V

L

L

V

F

F

G

G

V

L

V

V

L

I

S

G

A

K

I

M

K

G

K

E

D

K

P

G

R

Q

V

I

T

-

P

-

L

L

I

V

A

D

G

F

I

F

D

N

A

A

L

L

V

S

P

D

A

A

M

T

L

M

K

K

L

I

T

V

D

Q

Y

I

V

I

M

M

R

C

L

Y

A

M

P

P

I

L

G

G

V

I

F

L

G

F

A

L

L

I

A

A

S

G

A

K

I

I

-

I

T

E

V

V

N

E

G

D

L

W

D

E

V

I

L

F

T

R

T

K

Y

L

G

G

K

L

L

Y

V

M

G

A

S

T

F

V

Y

L

L

T

G

G

L

L

V

A

T

I

L

H

W

S

I

I

V

V

L

I

I

L

-

P

F

L

V
x
I

G

Y

Y

F

A

I

F

V

L

V

G

R

K

K

S

N

I

P

W

F

R

R

L

F

L

A

K

M

A

G

V

M

R

A

E

Q

P

A

A

L

M

L

L

T

A

A

F

L

S

M

T

I

A

S

S

S

S
|
S

E

S

A

A

A

T

Y

L

P

P

-

V

-

T

-

F

R

R

L

C

T

A

E

E

K

-

L

-

E

E

A

N

F

N

G

Q

I

V

D

D

K

K

K

R

V

I

V

T

G

R

F

F

T

V

L

L

P

P

L

V

G

G

Y

A

A

T

F

I

N

N

L

M

D

D

G

G

S

T

M

A

M

L

Y

Y

Q

E

A

A

F

V

A

A

I

V

F

F

I

I

A

A

Q

Q

A

L

F

N

G

D

I

L

D

D

M

L

P

G

L

I

G

G

A

Q

Q

I

I

I

M

T

M

I

L

S

L

I

V

T

L

A

M

T

L

S

S

A

S

S

K

I

G

G

M

A

A

A

G

G

V

V

A
x
P

R

Q

G

A

S

G

V

L

V

V

V

T

V

M

A

V

A

I

I

V

A

L

P

S

M

A

F

V

H

G

L

L

P

P

P

A

S

E

G

D

V

V

V

T

L

L

I

I

L

I

A

A

I

V

D

D

Q

W

I

L

L

L

D
|
D

M

R

G

F

R
|
R

T
|
T

A

M

T

V

N
|
N

V

V

I

L

G

G

N

D

S

A

I

F

A

G

T

T

A

G

V

I

I

V

A

E

K

K

W
x
L

E

-

A

S

K

K

R

K

A

E

V

L

K

E

H

Q

I

M

D

D

binding (+)-3-Hydroxy-4,5,6,6a-tetrahydro-3aH-pyrrolo[3,4-d]isoxazole-6-carboxylic acid: S287 (= S273), D398 (= D383), R401 (= R386), T402 (= T387), N405 (= N390)
binding (-)-3-Hydroxy-4,5,6,6a-tetrahydro-3aH-pyrrolo[3,4-d]isoxazole-6-carboxylic acid: P366 (≠ A351), D398 (= D383), R401 (= R386), T402 (= T387)
binding cholesterol hemisuccinate: I258 (≠ V244), L419 (≠ W404)

6s3qA Structure of human excitatory amino acid transporter 3 (eaat3) in complex with tfb-tboa
26% identity, 95% coverage: 2:414/435 of query aligns to 8:428/430 of 6s3qA

query
sites
6s3qA

K

N

N

N

R

W

L

V

T

L

F

L

Y

S

I

T

V

V

A

A

G

A

M

V

A

V

L

L

G

G

V

I

I

T

V

T

G

G

Y

V

V

L

C

V

-

R

-

E

H

H

R

R

S

N

A

L

A

S

G

T

A

L

A

E

E

K

A

F

K

-

T

-

I

-

A

-

G

-

Y

Y

F

F

S

A

I

F

I

P

T

G

D

E

I

I

F

L

L

M

R

R

L

M

V

L

K

K

M

L

I

I

A

L

P

P

L

L

V

I

F

I

A

S

T

S

L

M

V

I

S

T

G

G

L

V

A

A

G

A

M

L

E

D

G

-

T

-

S

S

D

N

V

V

R

S

-

G

R
x
K

I

I

G

G

F

V

R
|
R

S

A

V

V

G

V

W

Y

F

Y

V

F

C

C

A

T

S

T

L

L

F

I

S

A

L

V

A

I

L

L

G

G

L

I

A

V

L

L

A

V

N

V

A

S

L

I

Q

K

P

P

G

G

A

-

G

-

L

-

H

-

M

V

T

T

Q

Q

T

V

S

S

S

T

D

V

V

D

A

A

T

-

G

-

L

-

N

-

T

-

A

-

G

-

L

-

N

-

F

M

K

L

D

D

F

L

V

I

T

R

H

N

A

M

F

F

P

P

S

E

S

N

I

L

I

V

D

Q

A

A

M

C

A

F

R

Q

N

Q

-

Y

-

K

-

T

-

K

-

R

-

E

-

V

-

K

-

P

-

K

-

T

-

K

-

E

-

Y

-

K

-

I

-

V

-

G

-

M

-

Y

-

S

-

D

-

G

-

I

D

N

I

V

L

L

Q

G

I

L

L

I

V

V

F

F

S

C

V

L

L

V

F

F

G

G

V

L

V

V

L

I

S

G

A

K

I

M

K

G

K

E

D

K

P

G

R

Q

V

I

T

-

P

-

L

L

I

V

A

D

G

F

I

F

D

N

A

A

L

L

V

S

P

D

A

A

M

T

L

M

K

K

L

I

T

V

D

Q

Y

I

V

I

M

M

R

C

L

Y

A

M

P

P

I

L

G

G

V

I

F

L

G

F

A

L

L

I

A

A

S

G

A

K

I

I

-

I

T

E

V

V

N

E

G

D

L

W

D

E

V

I

L

F

T

R

T

K

Y

L

G

G

K

L

L

Y

V

M

G

A

S

T

F

V

Y

L

L

T

G

G

L

L

V

A

T

I

L

H

W

S

I

I

V

V

L

I

I

L

-

P

F

L

V

I

G

Y

Y

F

A

I

F

V

L

V

G

R

K

K

S

N

I

P

W

F

R

R

L

F

L

A

K

M

A

G

V

M

R

A

E

Q

P

A

A

L

M

L

L

T

A

A

F

L

S

M

T

I

A

S

S
|
S

E

S

A

A

A

T

Y

L

P

P

-

V

-

T

-

F

R

R

L

C

T

A

E

E

K

-

L

-

E

E

A

N

F

N

G

Q

I

V

D

D

K

K

K

R

V

I

V

T

G

R

F

F

T

V

L

L

P

P

L

V

G

G

Y

A

A

T

F

I

N

N

L

M

D

D

G

G

S
x
T

M

A

M

L

Y

Y

Q

E

A

A

F

V

A

A

I

V

F

F

I

I

A

A

Q

Q

A

L

F

N

G

D

I

L

D

D

M

L

P

G

L

I

G

G

A

Q

Q

I

I

I

M

T

M

I

L

S

L

I

V

T

L

A

M

T

L

S

S
x
A

S

S

K

I

G
|
G

M

A

A

A

G

G

V
|
V

A

P

R

Q

G
x
A

S

G

V

L

V

V

V
x
T

V

M

A

V

A

I

I

V

A

L

P

S

M

A

F

V

H

G

L

L

P

P

P

A

S

E

G

D

V

V

V

T

L

L

I

I

L

I

A

A

I

V

D

D

Q

W

I

L

L

L

D
|
D

M

R

G

F

R
|
R

T
|
T

A

M

T

V

N
|
N

V

V

I

L

G

G

N

D

S

A

I

F

A

G

T

T

A

G

V

I

I

V

A

E

K

K

W

L

E

-

A

S

K

K

R

K

A

E

V

L

K

E

H

Q

I

M

D

D

binding (2~{S},3~{S})-2-azanyl-3-[[3-[[4-(trifluoromethyl)phenyl]carbonylamino]phenyl]methoxy]butanedioic acid: S286 (= S272), S287 (= S273), T324 (≠ S309), A358 (≠ S343), G361 (= G346), V365 (= V350), A368 (≠ G353), T372 (≠ V357), D398 (= D383), R401 (= R386), T402 (= T387), N405 (= N390)
binding cholesterol hemisuccinate: K80 (≠ R78), R84 (= R82)

5mjuA Structure of the thermostabilized eaat1 cryst mutant in complex with the competititve inhibitor tfb-tboa and the allosteric inhibitor ucph101 (see paper)
27% identity, 91% coverage: 16:412/435 of query aligns to 25:395/397 of 5mjuA

query
sites
5mjuA

G

G

V

V

I

V

V

L

G

G

Y

F

V

L

C

L

H

R

R

P

S

Y

A

P

A

L

G

S

A

P

A

R

E

E

A

V

K

K

T

-

I

-

A

-

G

-

Y

Y

F

F

S

A

I

F

I

P

T

G

D

E

I

L

F

L

L

M

R

R

L

M

V

L

K

K

M

M

I

L

I

I

A

L

P

P

L

L

V
x
I

F

V

A

S

T

S

L

L

V

I

S

T

G

G

L
|
L

A

A

G

S

M

L

E

D

G

A

T

K

S

A

D
x
S

V

-

R
x
G

R

R

I

L

G
|
G

F

M

R

R

S

A

V

V

G

V

W

Y

F
x
Y

V

M

C

S

A

T

S

T

L

I

F

I

S

A

L

V

A

V

L

L

G

G

L

I

A

I

L

L

A

-

N

-

A

-

L

-

Q

-

P

-

G

-

A

-

G

-

L

V

H

L

M

I

T

I

Q

H

T

E

S

V

S

L

D

D

V

C

A

-

T

-

G

-

L

-

N

-

T

-

A

-

G

-

L

-

N

-

F

F

K

L

D

D

F

L

V

A

T

R

H

N

A

I

F

F

P

P

S

S

S

N

I

L

I

V

D

S

A

A

M

A

A

F

R

R

N

S

-

Y

-

S

-

T

-

G

-

Q

-

E

-

V

-

E

-

G

-

M

D

N

I
|
I

L

L

Q

G

I

L

L

V

V

V

F

F

S

S

V
x
M

L

V

F

F

G

G

V
x
F

V

A

L

L

S

G

A

K

I

M

K

G

K

E

D

Q

P

G

R

Q

V

L

T

-

P

-

L

L

I

V

A

D

G

F

I

F

D

N

A

S

L

L

V

N

P

E

A

A

M

T

L

M

K

K

L

L

T

V

D

A

Y

I

V

I

M

M

R

W

L

Y

A

A

P

P

I

L

G

G

V

I

F

L

G

F

A

L

L

I

A

A

S

G

A

K

I

I

T

-

V

V

N

E

G

G

L

Q

D

L

V

G

L

M

T

Y

T

M

Y

V

G

T

K

V

L

I

V

V

G

G

S

L

F

V

Y

I

L

H

G

G

L

L

V

-

T

-

L

-

W

-

I

I

V

V

L

L

I

P

F

L

V

I

G

Y

Y

F

A

L

F

I

L

T

G

R

K

K

S

N

I

P

W

F

R

V

L

F

L

I

K

A

A

G

V

I

R

L

E

Q

P

A

A

L

M

I

L

T

A

A

F

L

S

G

T

T

A

S

S

S

S
|
S

E
x
S

A

A

A

T

Y

L

P

P

R

I

L

T

T

F

E

K

K

C

L

L

E

E

A

E

F

N

-

N

G

G

I

V

D

D

K

K

K

R

V

I

V

T

G

R

F
|
F

T

V

L

L

P

P

L
x
V

G

G

Y
x
A

A
x
T

F

I

N

N

L
x
M

D

D

G

G

S
x
T

M

A

M

L

Y

Y

Q

E

A

A

F

V

A

A

I

I

F

F

I

I

A

A

Q

Q

A

V

F

N

G

Q

I

I

D

-

M

-

P

-

L

-

G

-

A

-

Q

-

I

I

M

T

M

I

L

S

L

I

V

T

L

A

M

T

L

A

S

A

S

S

K

I

G
|
G

M

A

A

A

G

G

V

I

A

P

R

Q

G
x
A

S
x
G

V

L

V

V

V

T

V

M

A

V

A

I

I

V

A

L

P

T

M

A

F

V

H

G

L

L

P

P

P

T

S

D

G

D

V

I

V

T

L

L

I

I

L

I

A

A

I

V

D

D

Q

W

I

L

L

L

D
|
D

M

R

G

F

R
|
R

T

T

A

M

T

V

N
|
N

V

V

I

L

G

G

N

D

S

A

I

L

A

G

T

A

A

G

V

I

I

V

A

E

K

H

W

L

E

S

A

R

K

K

R

E

A

L

V

E

K

K

H

Q

binding 2-Amino-5,6,7,8-tetrahydro-4-(4-methoxyphenyl)-7-(naphthalen-1-yl)-5-oxo-4H-chromene-3-carbonitrile: L72 (= L67), S80 (≠ D75), G81 (≠ R77), G84 (= G80), Y91 (≠ F87), M156 (≠ V160), F160 (≠ V164), F286 (= F296), V290 (≠ L300)
binding (2~{S},3~{S})-2-azanyl-3-[[3-[[4-(trifluoromethyl)phenyl]carbonylamino]phenyl]methoxy]butanedioic acid: I64 (≠ V59), I148 (= I152), S262 (= S273), S263 (≠ E274), A292 (≠ Y302), T293 (≠ A303), M296 (≠ L306), T299 (≠ S309), G329 (= G346), A336 (≠ G353), G337 (≠ S354), D366 (= D383), R369 (= R386), N373 (= N390)

Query Sequence

>BPHYT_RS33330 BPHYT_RS33330 C4-dicarboxylate ABC transporter
MKNRLTFYIVAGMALGVIVGYVCHRSAAGAAEAKTIAGYFSIITDIFLRLVKMIIAPLVF
ATLVSGLAGMEGTSDVRRIGFRSVGWFVCASLFSLALGLALANALQPGAGLHMTQTSSDV
ATGLNTAGLNFKDFVTHAFPSSIIDAMARNDILQILVFSVLFGVVLSAIKKDPRVTPLIA
GIDALVPAMLKLTDYVMRLAPIGVFGALASAITVNGLDVLTTYGKLVGSFYLGLVTLWIV
LIFVGYAFLGKSIWRLLKAVREPAMLAFSTASSEAAYPRLTEKLEAFGIDKKVVGFTLPL
GYAFNLDGSMMYQAFAAIFIAQAFGIDMPLGAQIMMLLVLMLSSKGMAGVARGSVVVVAA
IAPMFHLPPSGVVLILAIDQILDMGRTATNVIGNSIATAVIAKWEAKRAVKHIDGADSPA
LGRLQELRELQGETK

Or try a new SitesBLAST search

SitesBLAST's Database

SitesBLAST's database includes (1) SwissProt entries with experimentally-supported functional features; and (2) protein structures with bound ligands, from the BioLip database.

by Morgan Price, Arkin group
Lawrence Berkeley National Laboratory