SitesBLAST

SitesBLAST – Find functional sites

SitesBLAST

Comparing BPHYT_RS33490 FitnessBrowser__BFirm:BPHYT_RS33490 to proteins with known functional sites using BLASTp with E ≤ 0.001.

Or try Sites on a Tree, PaperBLAST, Conserved Domains, or compare to all protein structures

Found 20 (the maximum) hits to proteins with known functional sites (download)

Q5SKN9 Long-chain-fatty-acid--CoA ligase; Long-chain fatty acyl-CoA synthetase; LC-FACS; EC 6.2.1.3 from Thermus thermophilus (strain ATCC 27634 / DSM 579 / HB8) (see paper)
35% identity, 95% coverage: 23:538/543 of query aligns to 22:535/541 of Q5SKN9

query
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Q5SKN9

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T184 (= T190) binding
G302 (= G305) binding
Q322 (≠ H325) binding
G323 (≠ V326) binding
T327 (= T330) binding
E328 (= E331) binding
D418 (= D422) binding
K435 (= K439) binding
K439 (≠ I443) binding

6ijbB Structure of 3-methylmercaptopropionate coa ligase mutant k523a in complex with amp and mmpa (see paper)
31% identity, 96% coverage: 18:538/543 of query aligns to 9:534/538 of 6ijbB

query
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6ijbB

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active site: T185 (= T190), H205 (≠ N210), H231 (= H234), S329 (≠ T330), E330 (= E331), K438 (≠ I443), W443 (≠ N448), A523 (≠ K527)
binding 3-(methylsulfanyl)propanoic acid: W235 (= W238), G303 (= G305), A325 (≠ V326), W326 (≠ Y327), G327 (= G328), M328 (≠ L329)
binding adenosine monophosphate: G303 (= G305), A304 (= A306), A305 (= A307), H324 (= H325), W326 (≠ Y327), G327 (= G328), M328 (≠ L329), S329 (≠ T330), Q359 (= Q360), D417 (= D422)

1v26B Crystal structure of tt0168 from thermus thermophilus hb8 (see paper)
34% identity, 92% coverage: 23:523/543 of query aligns to 15:496/510 of 1v26B

query
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1v26B

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S

K

E

H

K

A

D

I

V

Y

V

L

L

W

P

T

V

L

V

P

P

L

M

F

F

H
|
H

C

V

N

N

G

A

W

W

C

C

F

L

P

P

W

Y

T

A

V

-

A

A

A

T

R

L

A

V

G

G

V

A

N

K

V

Q

C

V

L

L

-

P

-

G

R

P

K

R

F

L

D

D

A

P

K

A

T

S

V

L

F

V

D

E

L

L

I

F

R

D

R

G

E

E

G

G

I

V

T

T

H

F

Y

T

C

A

G

G

A

V

P

P

I

T

V

V

Q

W

S

L

A

A

L

L

A

A

N

D

A

-

P

-

A

-

Q

-

W

Y

R

L

E

E

G

S

I

T

E

G

H

H

R

R

V

L

S

K

T

T

M

L

-

R

-

L

-

V

V

V

A

G

G
|
G

A
x
S

A
|
A

P

A

A

P

P

R

A

S

V

L

I

I

A

A

K

R

M

F

K

E

E

R

I

M

G

G

F

V

D

E

L

V

T

R

H

Q

V
x
G

Y
|
Y

G
|
G

L
|
L

T
|
T

E
|
E

T

T

Y

S

G

P

P

V

A

V

V

Q

C

N

A

F

K

V

Q

K

E

S

E

H

W

L

E

E

A

S

L

L

D

S

D

E

G

E

A

E

R

K

A

L

E

T

M

L

N

K

A

A

R

K

Q

T

G

G

V

L

R

P

Y

I

H

P

L

L

Q

-

A

V

A

R

V

L

T

R

V

V

L

A

D

D

P

E

D

E

T

G

L

-

T

R

P

P

V

V

P

P

D

K

D

D

G

G

E

K

T

A

I

L

G

G

E

E

I

V

M

Q

F

L

R

K

G

G

N

P

I

W

C

I

M

T

K

G

G

G

Y

Y

L

Y

K

G

N

N

E

E

R

E

A

A

T

T

E

R

A

S

T

A

F

L

Q

T

-

P

G

D

G

G

W

F

F

F

H

R

T

T

G

G

D
|
D

L

I

G

A

V

V

R

W

M

D

P

E

D

E

G

G

Y

Y

I

V

R

E

I

I

R

K

D

D

R

R

S

L

K
|
K

D

D

I

L

I

I

I
x
K

S

S

G

G

E

E

N
x
W

I

I

S

S

I

V

E

D

V

L

E

E

D

N

T

A

L

-

Y

-

R

-

H

-

P

-

A

-

V

-

S

-

V

-

A

A

V

V

A

A

M

I

A

P

D

H

P

P

K

K

W

W

G

Q

E

E

V

R

P

P

C

L

A

A

F

-

V

-

E

-

L

-

K

-

E

-

G

-

A

-

Q

-

V

V

S

T

A

P

E

E

I

L

I

N

A

E

H

H

-

L

C

L

R

K

L

A

F

G

L

F

A

A

G

K

Y

W

K

Q

L

L

P

P

K

D

A

A

V

Y

R

V

F

F

G

A

-

E

E

E

L

I

P

P

K

R

T

T

active site: T177 (= T190), H197 (≠ N210), H223 (= H234), T320 (= T330), E321 (= E331), K432 (≠ I443), W437 (≠ N448)
binding adenosine monophosphate: G295 (= G305), S296 (≠ A306), A297 (= A307), G316 (≠ V326), Y317 (= Y327), G318 (= G328), L319 (= L329), T320 (= T330), D411 (= D422), K428 (= K439), K432 (≠ I443), W437 (≠ N448)
binding magnesium ion: T177 (= T190), E321 (= E331)

1v25A Crystal structure of tt0168 from thermus thermophilus hb8 (see paper)
34% identity, 85% coverage: 23:485/543 of query aligns to 15:464/491 of 1v25A

query
sites
1v25A

D

D

F

F

I

L

V

E

R

R

A

A

E

A

V

L

Y

F

G

G

E

R

R

K

P

E

A

V

V

V

V

S

H

R

-

L

-

H

-

T

G

G

E

E

I

V

R

H

R

R

N

T

-

T

W

Y

R

A

E

E

T

V

Y

Y

E

Q

R

R

A

A

R

R

L

L

A

M

S

G

A

G

L

L

Q

R

Q

A

A

L

G

G

I

V

Q

G

R

V

G

G

D

D

T

R

V

V

A

A

A

T

L

L

L

G

P

F

N

N

I

H

P

F

P

R

M

H

I

L

E

E

A

A

H

Y

F

F

G

A

V

V

P

P

M

G

A

M

G

G

A

A

V

V

L

L

N

H

T

T

L

A

N

N

T

P

R

R

L

L

D

S

V

P

S

K

S

E

L

I

L

A

F

Y

M

I

L

L

R

N

H

H

G

A

E

E

A

D

K

K

A

V

L

L

I

L

V

F

D

D

T

P

E

N

Y

L

G

L

E

P

F

L

A

V

H

E

R

A

A

I

A

R

L

G

E

E

F

L

P

K

D

T

L

V

R

Q

V

H

I

F

S

V

V

V

A

M

D

D

A

E

M

-

P

-

A

K

D

A

P

P

S

E

Q

G

F

Y

I

L

R

A

A

Y

T

E

D

-

Y

-

E

E

A

A

F

L

L

G

Q

E

S

E

G

A

D

D

P

P

E

-

F

-

A

-

W

-

A

-

M

V

P

R

A

V

D

P

E

E

W

R

D

A

A

A

I

C

A

G

L

M

N

A

Y

Y

T
|
T

S

T

G

G

T

T

G

G

D

L

P

P

K

K

G

G

V

V

Y

Y

H

S

H

H

R

R

G

A

A

L

Y

V

L

L

N
x
H

A

S

L

L

S

A

N

A

I

S

L

L

-

V

-

D

E

G

W

T

D

A

M

L

P

S

K

E

H

K

A

D

I

V

Y

V

L

L

W

P

T

V

L

V

P

P

L

M

F

F

H
|
H

C
x
V

N

N

G

A

W

W

C

C

F

L

P

P

W

Y

T

A

V

-

A

A

A

T

R

L

A

V

G

G

V

A

N

K

V

Q

C

V

L

L

-

P

-

G

R

P

K

R

F

L

D

D

A

P

K

A

T

S

V

L

F

V

D

E

L

L

I

F

R

D

R

G

E

E

G

G

I

V

T

T

H

F

Y

T

C

A

G

G

A

V

P

P

I

T

V

V

Q

W

S

L

A

A

L

L

A

A

N

D

A

-

P

-

A

-

Q

-

W

Y

R

L

E

E

G

S

I

T

E

G

H

H

R

R

V

L

S

K

T

T

M

L

-

R

-

L

-

V

V

V

A

G

G
|
G

A
x
S

A
|
A

P

A

A

P

P

R

A

S

V

L

I

I

A

A

K

R

M

F

K

E

E

R

I

M

G

G

F

V

D

E

L

V

T

R

H

Q

V

G

Y
|
Y

G
|
G

L
|
L

T
|
T

E
|
E

T

T

Y

S

G

P

P

V

A

V

V

Q

C

N

A

F

K

V

Q

K

E

S

E

H

W

L

E

E

A

S

L

L

D

S

D

E

G

E

A

E

R

K

A

L

E

T

M

L

N

K

A

A

R

K

Q

T

G

G

V

L

R

P

Y

I

H

P

L

L

Q

-

A

V

A

R

V

L

T

R

V

V

L

A

D

D

P

E

D

E

T

G

L

-

T

R

P

P

V

V

P

P

D

K

D

D

G

G

E

K

T

A

I

L

G

G

E

E

I

V

M

Q

F

L

R

K

G

G

N

P

I

W

C

I

M

T

K

G

G

G

Y

Y

L

Y

K

G

N

N

E

E

R

E

A

A

T

T

E

R

A

S

T

A

F

L

Q

T

-

P

G

D

G

G

W

F

F

F

H

R

T

T

G

G

D
|
D

L

I

G

A

V

V

R

W

M

D

P

E

D

E

G

G

Y

Y

I

V

R

E

I
|
I

R

K

D

D

R

R

S

L

K

K

D

D

I

L

I

I

I
x
K

S

S

G

G

E

E

N
x
W

I

I

S

S

I

V

E

D

V

L

E

E

D

N

T

-

L

-

Y

-

R

-

H

-

P

-

A

-

V

-

S

-

V

-

A

A

V

V

A

A

M

I

A

P

D

H

P

P

K

K

W

W

G

Q

E

E

V

R

P

P

C

L

A

A

F

V

V

V

active site: T177 (= T190), H197 (≠ N210), H223 (= H234), T320 (= T330), E321 (= E331), K432 (≠ I443), W437 (≠ N448)
binding phosphoaminophosphonic acid-adenylate ester: H223 (= H234), V224 (≠ C235), G295 (= G305), S296 (≠ A306), A297 (= A307), Y317 (= Y327), G318 (= G328), L319 (= L329), T320 (= T330), D411 (= D422), I423 (= I434), K432 (≠ I443), W437 (≠ N448)
binding magnesium ion: T177 (= T190), E321 (= E331)

6ihkB Structure of mmpa coa ligase in complex with adp (see paper)
31% identity, 96% coverage: 18:538/543 of query aligns to 9:531/533 of 6ihkB

query
sites
6ihkB

P

P

L

L

T

L

P

I

I

S

D

S

F

L

I

I

V

D

R

H

A

A

E

R

V

Y

Y

H

G

G

E

Q

R

T

P

E

A

I

V

V

-

S

-

V

-

E

V

T

H

D

G

G

E

T

I

V

R

T

R

R

-

T

N

N

W

W

R

G

E

E

T

I

Y

A

E

A

R

N

A

A

R

R

L

M

A

G

S

S

A

A

L

L

Q

T

Q

K

A

L

G

G

I

L

Q

Q

R

P

G

Q

D

D

T

R

V

I

A

G

A

T

L

L

L

A

P

W

N

N

I

N

P

R

M

H

I

L

E

E

A

I

H

Y

F

Y

G

A

V

A

P

S

M

G

A

A

G

G

A

F

V

V

L

C

N

H

T

T

L

I

N

N

T

P

R

R

L

L

D

F

V

P

S

E

S

Q

L

L

L

V

F

Y

M

I

L

I

R

N

H

H

G

A

E

Q

A

D

K

R

A

V

L

L

I

F

V

F

D

D

T

A

E

T

Y

F

G

L

E

P

F

L

A

V

H

A

R

A

A

I

A

R

L

D

E

Q

F

L

P

T

D

E

L

V

R

K

V

H

I

F

S

V

V

L

A

M

D

G

A

P

M

R

P

N

A

E

D

D

P

A

S

L

Q

Q

F

Q

I

I

R

P

A

G

T

L

D

E

-

F

Y

Y

E

D

A

E

F

L

L

I

Q

E

S

T

G

G

D

D

P

T

E

D

F

F

A

E

W

W

A

P

M

V

P

-

A

F

D

D

E

E

W

N

D

T

A

A

I

S

A

S

L

L

N

C

Y

Y

T
|
T

S

S

G

G

T

-

G

-

D

H

P

P

K

K

G

G

V

V

V

L

Y

Y

H

S

H

H

R

R

G

S

A

T

Y

V

L

L

N
x
H

A

S

L

F

S

A

N

S

I

N

L

T

E

R

W

-

D

D

M

V

P

I

K

G

H

Y

A

S

-

A

-

M

-

D

I

V

Y

V

L

M

W

P

T

V

L

V

P

P

L

M

F

F

H
|
H

C

V

N

N

G

A

W

W

C

G

F

S

P

P

W

Y

T

G

V

-

A

C

A

A

R

M

A

S

G

G

V

A

N

Q

V

M

C

V

L

L

R

P

K

G

F

P

D

D

-

L

-

H

A

G

K

E

T

A

V

L

F

V

D

N

L

L

I

I

R

D

R

T

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Y

G

G

I

V

T

T

H

L

Y

A

C

M

G

G

A

V

P

P

I

T

V

I

Q

W

S

Q

A

G

L

L

A

L

N

A

A

H

P

A

A

A

Q

K

W

C

R

G

E

T

G

K

I

L

E

E

H

S

R

L

V

E

S

R

T

T

M

V

V

I

A

G

G
|
G

A
|
A

P

C

A

P

P

P

A

S

V

M

I

I

A

A

K

T

M

F

K

R

E

E

-

K

I

Y

G

G

F

V

D

D

L

T

T

V

H
|
H

V
x
A

Y
x
W

G
|
G

L
x
M

T
x
S

E
|
E

T

M

Y

S

G

P

P

L

A

G

A

T

V

A

C

N

A

I

K

P

Q

L

E

A

E

K

W

H

E

R

A

K

L

L

D

P

D

I

G

E

A

E

R

Q

A

H

E

K

M

L

N

R

A

E

R

N

Q
|
Q

G

G

V

-

R

R

Y

P

H

P

L

F

Q

G

A

V

A

E

V

L

T

K

V

I

L

V

D

D

P

D

D

D

T

G

L

-

T

N

P

D

V

L

P

P

D

H

D

D

G

G

E

V

T

T

I

Q

G

G

E

D

I

L

M

M

F

V

R

R

G

G

N

H

I

W

C

V

M

L

K

D

G

S

Y

Y

L

F

K

-

N

-

E

Q

R

L

A

K

T

D

E

Q

A

E

T

L

F

L

Q

Q

G

D

G

G

W

W

F

F

H

A

T

T

G

G

D
|
D

L

V

G

A

V

T

R

L

M

D

P

P

D

D

G

G

Y

Y

I

M

R

T

I

I

R

R

D

D

R
|
R

S

S

K

K

D

D

I

I

I
x
K

S

S

G

G

E

E

N
x
W

I

I

S

S

I

V

E

E

V

L

E

E

D

N

T

I

L

A

Y

V

R

A

H

H

P

P

A

K

V

L

S

A

V

T

A

A

V

V

V

I

A

G

M

V

A

P

D

H

P

P

K

K

W

W

G

D

E

E

V

R

P

P

C

L

A

L

F

V

V

A

E

V

L

K

K

A

E

E

G

G

A

E

Q

D

V

P

S

S

A

E

E

A

E

E

I

L

A

E

H

F

C

F

R

D

L

G

F

K

L

I

A

A

G

K

Y

W

K

Q

L

V

P

P

K

D

A

K

V

V

R

V

F

F

G

V

E

D

-

A

L

L

P

P

K

L

T

N

S

A

T

T

G

G

K
|
K

I

V

Q

L

K

K

F

R

E

K

L

L

R

R

A

D

R

E

I

F

K

K

active site: T185 (= T190), H202 (≠ N210), H228 (= H234), S326 (≠ T330), E327 (= E331), K435 (≠ I443), W440 (≠ N448), K520 (= K527)
binding adenosine-5'-diphosphate: H228 (= H234), G300 (= G305), A301 (= A306), A302 (= A307), H321 (= H325), A322 (≠ V326), W323 (≠ Y327), G324 (= G328), M325 (≠ L329), S326 (≠ T330), Q356 (= Q360), D414 (= D422), R429 (= R437), K520 (= K527)

5gtdA O-succinylbenzoate coa synthetase (mene) from bacillus subtilis in complex with the acyl-adenylate intermediate osb-amp (see paper)
30% identity, 95% coverage: 23:540/543 of query aligns to 6:483/484 of 5gtdA

query
sites
5gtdA

D

N

F

W

I

L

V

M

R

Q

A

R

A

A

E

Q

V

L

Y

T

G

P

E

E

R

R

P

I

A

A

V

L

V

I

H

Y

G

E

E

D

I

Q

R

T

R

V

N

T

W

F

R

A

E

E

T

L

Y

F

E

A

R

A

A

S

R

K

R

R

L

M

A

A

S

E

A

Q

L

L

Q

A

A

H

G

S

I

V

Q

R

R

K

G

G

D

D

T

T

V

A

A

A

A

I

L

L

P

Q

N

N

I

R

P

A

P

E

M

M

I

V

E

Y

A

A

H

V

F

H

G

A

V

C

P

F

M

L

A

L

G

G

A

V

V

K

L

A

N

V

T

L

L

L

N

N

T

T

R

K

L

L

D

S

V

T

S

H

S

E

L

R

L

L

F

F

M

Q

L

L

R

E

H

D

G

S

E

G

A

S

K

G

A

F

L

L

I

L

V

T

D

D

T

S

E

S

Y

F

-

E

-

K

G

K

E

E

F

Y

A

E

H

H

R

-

A

-

L

-

E

-

F

-

P

-

D

I

L

V

R

Q

V

T

I

I

S

D

V

V

A

D

D

E

A

L

M

M

P

K

A

E

D

A

P

A

S

E

Q

-

F

-

I

-

R

-

A

E

T

I

D

E

Y

I

E

E

A

A

F

Y

L

M

Q

Q

S

-

G

-

D

-

P

-

E

-

F

-

A

-

W

-

A

-

M

-

P

-

A

-

D

-

E

-

W

M

D

D

A

A

I

T

A

A

-

T

L

L

N

M

Y

Y

T
|
T

S

S

G

G

T

T

G

G

D

K

P

P

K

K

G

G

V

V

V

Q

Y

Q

H

T

H

F

R

G

G

N

A

H

Y

Y

L

F

N
x
S

A

A

L

V

S

S

N

S

I

A

L

L

E

N

W

L

D

G

M

I

P

T

K

E

H

Q

A

D

I

R

Y

W

L

L

W

I

T

A

L

L

P

P

L

L

F

F

H
|
H

C

I

N
x
S

G

G

W

L

C

S

F

A

P

L

W

F

T

K

V

S

A

V

A

I

R

Y

A

G

G

M

V

T

N

V

V

V

C

L

L

H

R

Q

K

R

F

F

D

S

A

V

K

S

T

D

V

V

F

L

D

H

L

S

I

I

R

N

R

R

E

H

G

E

I

V

T

T

H

M

Y

I

C

S

G
x
A

A

V

P

Q

I

T

V

M

Q

L

S

A

A

S

L

L

A

L

N

E

A

E

P

T

A

N

Q

R

W

C

R

P

E

E

G

S

I

I

E

-

H

-

R

R

V

C

S

I

T
x
L

M

L

V
x
G

A
x
G

G
|
G

A

P

A

A

P

P

A

L

P

P

A

-

V

L

I

L

A

E

K

E

M

C

K

R

E

E

I

K

G

G

F

F

D

P

L

V

T

F

H

Q

V

S

Y
|
Y

G
|
G

L
x
M

T
|
T

E
|
E

T

T

Y

C

G
x
S

P
x
Q

A

I

A

V

V

T

C

L

A

S

K

P

Q

E

E

F

E

S

W

M

E

E

A

K

L

L

D

G

D

S

G

A

A

G

R

K

A

P

E

L

M
x
F

N
x
S

A

C

R

E

Q

I

G

K

V

I

R

E

Y

R

H

-

L

-

Q

-

A

-

V

-

T

-

V

-

L

-

D

D

P

G

D

Q

T

V

L

C

T

E

P

P

V

Y

P

E

D

H

D

-

G

-

E

-

T

-

I

-

G

G

E

E

I

I

M

M

F

V

R

K

G

G

N

P

I

N

C

V

M

M

K

K

G

S

Y

Y

L

F

K

N

N

R

E

E

R

S

A

A

T

N

E

E

A

A

T

S

F

F

Q

Q

G

N

G

G

W

W

F

L

H

K

T

T

G

G

D
|
D

L

L

G

G

V

Y

R

L

M

D

P

N

D

E

G

G

Y

F

I

L

R

Y

I
x
V

R

L

D

D

R

R

S

R

K

S

D

D

I

L

I

I

S

S

G

G

E

E

N

N

I

I

S

Y

S

P

I

A

E

E

V

V

E

E

D

S

T

V

L

L

Y

L

R

S

H

H

P

P

A

A

V

V

S

A

V

E

A

A

A

G

V

V

V

S

A

G

M

A

A

E

D

D

P

K

K

K

W

W

G

G

E

K

V

V

P

P

C

H

A

A

F

Y

V

L

E

V

L

L

K

H

E

K

G

-

A

-

Q

P

V

V

S

S

A

A

E

G

E

E

I

L

I

T

A

D

H

Y

C

C

R

K

L

E

F

R

L

L

A

A

G

K

Y

Y

K

K

L

I

P

P

-

A

K

K

A

F

V

F

R

V

F

L

G

D

E

R

L

L

P

P

K

R

T

N

S

A

T

S

G

N

K

K

I

L

Q

L

K

R

F

N

E

Q

L

L

R

K

A

D

R

A

I

R

K

K

A

G

E

E

active site: T151 (= T190), S171 (≠ N210), H195 (= H234), T288 (= T330), E289 (= E331)
binding adenosine-5'-monophosphate: G263 (≠ A304), G264 (= G305), Y285 (= Y327), G286 (= G328), M287 (≠ L329), T288 (= T330), D366 (= D422), V378 (≠ I434)
binding magnesium ion: F314 (≠ M356), S315 (≠ N357)
binding 2-succinylbenzoate: H195 (= H234), S197 (≠ N236), A237 (≠ G276), L260 (≠ T301), G262 (≠ V303), G263 (≠ A304), G286 (= G328), M287 (≠ L329), S292 (≠ G334), Q293 (≠ P335)

5x8fB Ternary complex structure of a double mutant i454ra456k of o- succinylbenzoate coa synthetase (mene) from bacillus subtilis bound with amp and its product analogue osb-ncoa at 1.76 angstrom (see paper)
30% identity, 95% coverage: 23:540/543 of query aligns to 6:483/485 of 5x8fB

query
sites
5x8fB

D

N

F

W

I

L

V

M

R

Q

A

R

A

A

E

Q

V

L

Y

T

G

P

E

E

R

R

P

I

A

A

V

L

V

I

H
x
Y

G
x
E

E

D

I

Q

R

T

R

V

N

T

W

F

R

A

E

E

T

L

Y

F

E

A

R

A

A

S

R

K

R

R

L

M

A

A

S

E

A

Q

L

L

Q

A

A

H

G

S

I

V

Q

R

R

K

G

G

D

D

T

T

V

A

A

A

A

I

L

L

P

Q

N

N

I

R

P

A

P

E

M

M

I

V

E

Y

A

A

H

V

F
x
H

G

A

V

C

P

F

M

L

A

L

G

G

A

V

V

K

L

A

N

V

T

L

L

L

N

N

T

T

R
x
K

L

L

D

S

V

T

S

H

S

E

L

R

L

L

F

F

M

Q

L

L

R

E

H

D

G

S

E

G

A

S

K

G

A

F

L

L

I

L

V

T

D

D

T

S

E

S

Y

F

-

E

-

K

G

K

E

E

F

Y

A

E

H

H

R

-

A

-

L

-

E

-

F

-

P

-

D

I

L

V

R

Q

V

T

I

I

S

D

V

V

A

D

D

E

A

L

M

M

P

K

A

E

D

A

P

A

S

E

Q

-

F

-

I

-

R

-

A

E

T

I

D

E

Y

I

E

E

A

A

F

Y

L

M

Q

Q

S

-

G

-

D

-

P

-

E

-

F

-

A

-

W

-

A

-

M

-

P

-

A

-

D

-

E

-

W

M

D

D

A

A

I

T

A

A

-

T

L

L

N

M

Y

Y

T
|
T

S

S

G

G

T

T

G

G

D

K

P

P

K

K

G

G

V

V

V

Q

Y

Q

H

T

H

F

R

G

G

N

A

H

Y

Y

L

F

N
x
S

A

A

L

V

S

S

N

S

I

A

L

L

E
x
N

W

L

D

G

M

I

P

T

K

E

H

Q

A

D

I

R

Y

W

L

L

W

I

T

A

L
|
L

P
|
P

L

L

F

F

H
|
H

C
x
I

N
x
S

G

G

W

L

C

S

F

A

P
x
L

W

F

T

K

V

S

A

V

A

I

R

Y

A

G

G

M

V

T

N

V

V

V

C
x
L

L

H

R

Q

K

R

F

F

D

S

A

V

K

S

T

D

V

V

F

L

D

H

L

S

I

I

R

N

R

R

E

H

G

E

I

V

T

T

H

M

Y

I

C

S

G
x
A

A
x
V

P

Q

I

T

V

M

Q

L

S

A

A

S

L

L

A

L

N

E

A

E

P

T

A

N

Q

R

W

C

R

P

E

E

G

S

I

I

E

-

H

-

R

R

V

C

S

I

T
x
L

M

L

V
x
G

A

G

G

G

A

P

A

A

P

P

A

L

P

P

A

-

V

L

I

L

A

E

K

E

M

C

K

R

E

E

I

K

G

G

F

F

D

P

L

V

T

F

H

Q

V

S

Y

Y

G
|
G

L
x
M

T
|
T

E
|
E

T

T

Y

C

G
x
S

P
x
Q

A

I

A

V

V
x
T

C
x
L

A
x
S

K

P

Q

E

E

F

E

S

W

M

E

E

A

K

L

L

D

G

D

S

G

A

A

G

R

K

A

P

E

L

M

F

N

S

A

C

R

E

Q

I

G

K

V

I

R

E

Y

R

H

-

L

-

Q

-

A

-

V

-

T

-

V

-

L

-

D

D

P

G

D

Q

T

V

L

C

T

E

P

P

V

Y

P

E

D

H

D

-

G

-

E

-

T

-

I

-

G

G

E

E

I

I

M

M

F

V

R

K

G

G

N

P

I

N

C

V

M

M

K

K

G

S

Y

Y

L

F

K

N

N

R

E

E

R

S

A

A

T

N

E

E

A

A

T

S

F

F

Q

Q

G

N

G

G

W

W

F

L

H

K

T

T

G

G

D

D

L

L

G

G

V

Y

R

L

M

D

P

N

D

E

G

G

Y

F

I

L

R

Y

I

V

R

L

D

D

R

R

S

R

K

S

D

D

I

L

I

I

I
|
I

S
|
S

G
|
G

E
|
E

N
|
N

I

I

S

Y

S

P

I

A

E

E

V

V

E

E

D

S

T

V

L

L

Y

L

R

S

H

H

P

P

A

A

V

V

S

A

V

E

A

A

A

G

V

V

V

S

A

G

M

A

A

E

D

D

P

K

K
|
K

W
|
W

G

G

E

K

V

V

P

P

C

H

A

A

F

Y

V

L

E

V

L

L

K

H

E

K

G

-

A

-

Q

P

V

V

S

S

A

A

E

G

E

E

I

L

I

T

A

D

H

Y

C

C

R

K

L

E

F

R

L

L

A

A

G
x
K

Y
|
Y

K

K

L

R

P

P

K

K

A

K

-

F

V

F

R

V

F

L

G

D

E

R

L

L

P

P

K

R

T

N

S

A

T

S

G

N

K
|
K

I

L

Q

L

K

R

F

N

E

Q

L

L

R

K

A

D

R

A

I

R

K

K

A

G

E

E

active site: T151 (= T190), S171 (≠ N210), H195 (= H234), T288 (= T330), E289 (= E331), I387 (= I443), N392 (= N448), K470 (= K527)
binding magnesium ion: Y23 (≠ H40), E24 (≠ G41), H70 (≠ F87), N178 (≠ E217), L202 (≠ P241), L214 (≠ C253), T296 (≠ V338), L297 (≠ C339), S298 (≠ A340)
binding o-succinylbenzoyl-N-coenzyme A: K85 (≠ R102), L191 (= L230), P192 (= P231), H195 (= H234), I196 (≠ C235), S197 (≠ N236), A237 (≠ G276), V238 (≠ A277), L260 (≠ T301), G262 (≠ V303), G286 (= G328), M287 (≠ L329), S292 (≠ G334), Q293 (≠ P335), S388 (= S444), G389 (= G445), G390 (= G446), E391 (= E447), K420 (= K476), W421 (= W477), K450 (≠ G508), Y451 (= Y509)

P9WQ37 Long-chain-fatty-acid--CoA ligase FadD13; Fatty acyl-CoA ligase; FACL; FACL13; Fatty acyl-CoA synthetase; ACS; FACS; Very-long-chain fatty-acyl-CoA synthetase; ACSVL; EC 6.2.1.3 from Mycobacterium tuberculosis (strain ATCC 25618 / H37Rv) (see 4 papers)
31% identity, 92% coverage: 42:539/543 of query aligns to 27:499/503 of P9WQ37

query
sites
P9WQ37

E

D

I

V

R

R

R

M

N

T

W

Y

R

A

E

Q

T

M

Y

N

E

A

R

L

A

A

R

N

R

R

L

C

A

A

S

D

A

V

L

L

Q

T

Q

A

A

L

G

G

I

I

Q

A

R

K

G

G

D

D

T

R

V

V

A

A

A

L

L

L

L

M

P

P

N

N

I

S

P

V

P

E

M

F

I

C

E

C

A

L

H

F

F

Y

G

G

V

A

P

A

M

K

A

L

G

G

A

A

V

V

L

A

N

V

T

P

L

I

N

N

T

T

R

R

L

L

D

A

V

A

S

P

S

E

L

V

L

S

F

F

M

I

L

L

R

S

H

D

G

S

E

G

A

S

K

K

A

V

L

V

I

I

V

-

D

-

T

-

E

-

Y

Y

G

G

E

-

F

-

A

-

H

-

R

-

A

-

A

A

L

P

E

S

F

A

P

P

D

V

L

I

R

D

V

A

I

I

S

R

V

A

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K

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A

K172 (= K198) mutation to A: Slight reduction of the fatty acyl-CoA ligase activity. Slight increase of susceptibility to proteolysis.
R195 (≠ P221) mutation to A: Alteration of the strength of the membrane binding; when associated with A-9; A-17; A-197 and A-244.
R197 (≠ H223) mutation to A: Alteration of the strength of the membrane binding; when associated with A-9; A-17; A-195 and A-244.
V209 (≠ C235) mutation to D: Strong reduction of the fatty acyl-CoA ligase activity. No significant change in the total expression level, however the cytoplasmic expression is reduced. Slight increase of susceptibility to proteolysis.
A211 (≠ G237) mutation to G: Slight increase of the fatty acyl-CoA ligase activity. Reduced rate of proteolytic degradation.
T214 (≠ F240) mutation to W: Shows a marked decrease in the activity with lauric and palmitic acid (C12 and C16 fatty acid) with a simultaneous increase in the activity with caprylic acid (C8 fatty acid).
R244 (≠ G270) mutation to A: Alteration of the strength of the membrane binding; when associated with A-17; A-195; A-195 and A-197.
A302 (≠ G328) mutation to G: Slight increase of the fatty acyl-CoA ligase activity. Reduced rate of proteolytic degradation.; mutation to W: Does not show activity with small, medium or long acyl chains.
W377 (= W417) mutation to A: Strong reduction of the fatty acyl-CoA ligase activity. Enhanced affinity towards palmitic acid binding. No significant change in the total expression level, however the cytoplasmic expression is low. Slight increase of susceptibility to proteolysis.
D382 (= D422) mutation to A: Strong reduction of the fatty acyl-CoA ligase activity. No significant change in the total expression level, however the cytoplasmic expression is reduced.
R397 (= R437) mutation to A: Reduction of binding affinity for fatty acids.
S404 (= S444) mutation to A: Slight reduction of the fatty acyl-CoA ligase activity. Enhanced affinity towards palmitic acid binding.
G406 (= G446) mutation to L: No effect on the formation of acyl-adenylate intermediate. However, it shows very poor catalytic efficiency to form acyl-CoA.
K487 (= K527) mutation to A: Strong reduction of the fatty acyl-CoA ligase activity. Reduction of binding affinity for ATP.

Sites not aligning to the query:

9 R→A: Alteration of the strength of the membrane binding; when associated with A-9; A-195; A-197 and A-244.
17 R→A: Alteration of the strength of the membrane binding; when associated with A-9; A-17; A-197 and A-244.

5busA O-succinylbenzoate coenzyme a synthetase (mene) from bacillus subtilis, in complex with amp (see paper)
29% identity, 95% coverage: 23:540/543 of query aligns to 5:480/481 of 5busA

query
sites
5busA

D

N

F

W

I

L

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A

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A

A

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G

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D

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N

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L

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