SitesBLAST
Comparing BPHYT_RS34945 FitnessBrowser__BFirm:BPHYT_RS34945 to proteins with known functional sites using BLASTp with E ≤ 0.001.
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Found 20 (the maximum) hits to proteins with known functional sites (download)
1nfqA Rv2002 gene product from mycobacterium tuberculosis (see paper)
39% identity, 97% coverage: 6:253/255 of query aligns to 1:238/244 of 1nfqA
- active site: G17 (= G22), S139 (= S149), Y152 (= Y162), K156 (= K166)
- binding Androsterone: L91 (≠ T99), E141 (≠ T151), C149 (≠ L159), Y152 (= Y162), V193 (= V203), I197 (≠ R207), F198 (≠ H208)
- binding 1,4-dihydronicotinamide adenine dinucleotide: R16 (= R21), G17 (= G22), M18 (≠ L23), D37 (= D42), L39 (= L44), L59 (= L67), D60 (= D68), V61 (≠ L69), N87 (= N95), A88 (= A96), I137 (= I147), S139 (= S149), Y152 (= Y162), K156 (= K166), P182 (= P192), V185 (≠ T195), T187 (≠ V197), P188 (≠ E198), M189 (≠ A199), T190 (= T200)
1nffA Crystal structure of rv2002 gene product from mycobacterium tuberculosis (see paper)
39% identity, 97% coverage: 6:253/255 of query aligns to 1:238/244 of 1nffA
- active site: G17 (= G22), S139 (= S149), Y152 (= Y162), K156 (= K166)
- binding nicotinamide-adenine-dinucleotide: G13 (= G18), R16 (= R21), G17 (= G22), M18 (≠ L23), D37 (= D42), I38 (≠ V43), L39 (= L44), L59 (= L67), D60 (= D68), V61 (≠ L69), N87 (= N95), A88 (= A96), G89 (≠ A97), I90 (= I98), I137 (= I147), S139 (= S149), Y152 (= Y162), K156 (= K166), P182 (= P192), V185 (≠ T195), T187 (≠ V197), P188 (≠ E198), M189 (≠ A199), T190 (= T200)
P9WGT1 3-alpha-(or 20-beta)-hydroxysteroid dehydrogenase; NADH-dependent 3alpha, 20beta-hydroxysteroid dehydrogenase; EC 1.1.1.53 from Mycobacterium tuberculosis (strain ATCC 25618 / H37Rv) (see paper)
38% identity, 97% coverage: 6:253/255 of query aligns to 2:239/260 of P9WGT1
- I6 (≠ N10) mutation to T: Maximal improvement in solubility; when associated with M-47 and K-69.
- RGM 17:19 (≠ RGL 21:23) binding
- D38 (= D42) binding
- V47 (≠ L51) mutation to M: Maximal improvement in solubility; when associated with T-6 and K-69.
- DV 61:62 (≠ DL 68:69) binding
- T69 (≠ K76) mutation to K: Maximal improvement in solubility; when associated with T-6 and M-47.
- N88 (= N95) binding
- S140 (= S149) mutation to A: Complete loss of both oxidation of androsterone and reduction of progesterone; when associated with T6; M-47 and K-69.
- Y153 (= Y162) binding ; mutation to F: Complete loss of both oxidation of androsterone and reduction of progesterone; when associated with T6; M-47 and K-69.
- K157 (= K166) binding
- 183:191 (vs. 192:200, 44% identical) binding
4nbuB Crystal structure of fabg from bacillus sp (see paper)
34% identity, 96% coverage: 9:254/255 of query aligns to 5:244/244 of 4nbuB
- active site: G18 (= G22), N111 (= N121), S139 (= S149), Q149 (≠ L159), Y152 (= Y162), K156 (= K166)
- binding acetoacetyl-coenzyme a: D93 (≠ S101), K98 (≠ E108), S139 (= S149), N146 (≠ A156), V147 (≠ P157), Q149 (≠ L159), Y152 (= Y162), F184 (≠ L194), M189 (≠ A199), K200 (≠ Y210)
- binding 1,4-dihydronicotinamide adenine dinucleotide: G14 (= G18), N17 (≠ R21), G18 (= G22), I19 (≠ L23), D38 (= D42), F39 (≠ V43), V59 (≠ L67), D60 (= D68), V61 (≠ L69), N87 (= N95), A88 (= A96), G89 (≠ A97), I90 (= I98), T137 (≠ I147), S139 (= S149), Y152 (= Y162), K156 (= K166), P182 (= P192), F184 (≠ L194), T185 (= T195), T187 (≠ V197), M189 (≠ A199)
1vl8B Crystal structure of gluconate 5-dehydrogenase (tm0441) from thermotoga maritima at 2.07 a resolution
37% identity, 97% coverage: 9:255/255 of query aligns to 4:252/252 of 1vl8B
- active site: G17 (= G22), S143 (= S149), I154 (≠ L159), Y157 (= Y162), K161 (= K166)
- binding nadp nicotinamide-adenine-dinucleotide phosphate: G13 (= G18), R16 (= R21), G17 (= G22), L18 (= L23), S37 (≠ D42), R38 (≠ V43), C63 (≠ L67), D64 (= D68), V65 (≠ L69), A91 (≠ N95), A92 (= A96), G93 (≠ A97), I94 (= I98), V114 (= V120), I141 (= I147), S143 (= S149), Y157 (= Y162), K161 (= K166), P187 (= P192), G188 (= G193), Y190 (vs. gap), T192 (= T195), M194 (≠ V197), T195 (≠ E198)
3rwbA Crystal structure of complex of 4pal (4-pyridoxolactone) and pldh (tetrameric pyridoxal 4-dehydrogenase) from mesorhizobium loti
37% identity, 96% coverage: 9:253/255 of query aligns to 4:245/247 of 3rwbA
- active site: G17 (= G22), S140 (= S149), Y153 (= Y162), K157 (= K166)
- binding 7-hydroxy-6-methylfuro[3,4-c]pyridin-1(3H)-one: S140 (= S149), N141 (≠ D150), T142 (= T151), M150 (≠ L159), Y153 (= Y162), L185 (= L194), H196 (vs. gap)
- binding nicotinamide-adenine-dinucleotide: G13 (= G18), Q16 (≠ R21), G17 (= G22), I18 (≠ L23), D37 (= D42), I38 (≠ V43), D60 (= D68), I61 (≠ L69), N87 (= N95), A88 (= A96), S89 (≠ A97), I138 (= I147), S140 (= S149), Y153 (= Y162), K157 (= K166), P183 (= P192), L185 (= L194), I186 (≠ T195), S188 (≠ V197), G190 (≠ A199), V191 (≠ T200)
3ndrA Crystal structure of tetrameric pyridoxal 4-dehydrogenase from mesorhizobium loti
37% identity, 96% coverage: 9:253/255 of query aligns to 4:245/247 of 3ndrA
- active site: G17 (= G22), S140 (= S149), Y153 (= Y162), K157 (= K166)
- binding nicotinamide-adenine-dinucleotide: G13 (= G18), Q16 (≠ R21), G17 (= G22), I18 (≠ L23), D37 (= D42), I38 (≠ V43), D60 (= D68), I61 (≠ L69), N87 (= N95), A88 (= A96), S89 (≠ A97), V110 (= V120), I138 (= I147), S140 (= S149), Y153 (= Y162), K157 (= K166), P183 (= P192), L185 (= L194), I186 (≠ T195), S188 (≠ V197), G190 (≠ A199), V191 (≠ T200)
5itvA Crystal structure of bacillus subtilis bacc dihydroanticapsin 7- dehydrogenase in complex with nadh (see paper)
33% identity, 96% coverage: 9:252/255 of query aligns to 5:252/255 of 5itvA
- active site: G18 (= G22), S141 (= S149), Y154 (= Y162), K158 (= K166)
- binding 1,4-dihydronicotinamide adenine dinucleotide: G14 (= G18), S17 (≠ R21), G18 (= G22), I19 (≠ L23), D38 (= D42), I39 (≠ V43), T61 (≠ L67), I63 (≠ L69), N89 (= N95), G91 (≠ A97), T139 (≠ I147), S141 (= S149), Y154 (= Y162), K158 (= K166), P184 (= P192), G185 (= G193), I186 (≠ L194), I187 (≠ T195)
8cxaA Crystal structure of 3-oxoacyl-[acyl-carrier-protein] reductase from mycobacterium smegmatis with bound NAD
33% identity, 96% coverage: 9:252/255 of query aligns to 3:248/251 of 8cxaA
- binding nicotinamide-adenine-dinucleotide: G12 (= G18), Q15 (≠ R21), G16 (= G22), I17 (≠ L23), D36 (= D42), V63 (≠ L69), N89 (= N95), A91 (= A97), S94 (≠ N100), I142 (= I147), S143 (≠ A148), S144 (= S149), Y157 (= Y162), K161 (= K166), P187 (= P192), H188 (≠ G193), I190 (vs. gap), I194 (≠ V197)
4jroC Crystal structure of 3-oxoacyl-[acyl-carrier protein]reductase (fabg) from listeria monocytogenes in complex with NADP+
34% identity, 96% coverage: 9:254/255 of query aligns to 3:247/247 of 4jroC
- active site: G16 (= G22), S142 (= S149), Q152 (≠ L159), Y155 (= Y162), K159 (= K166)
- binding nadp nicotinamide-adenine-dinucleotide phosphate: G12 (= G18), S14 (≠ A20), R15 (= R21), G16 (= G22), I17 (≠ L23), N35 (≠ G41), Y36 (≠ D42), N37 (≠ V43), G38 (≠ L44), S39 (≠ H45), N63 (≠ D68), V64 (≠ L69), N90 (= N95), A91 (= A96), I93 (= I98), I113 (≠ V120), S142 (= S149), Y155 (= Y162), K159 (= K166), P185 (= P192), I188 (≠ T195), T190 (≠ V197)
2d1yA Crystal structure of tt0321 from thermus thermophilus hb8 (see paper)
39% identity, 95% coverage: 11:252/255 of query aligns to 5:236/240 of 2d1yA
- active site: G16 (= G22), S135 (= S149), N145 (≠ L159), Y148 (= Y162), K152 (= K166)
- binding nicotinamide-adenine-dinucleotide: G12 (= G18), R15 (= R21), I17 (≠ L23), D36 (= D42), L37 (= L44), R38 (≠ H45), V55 (≠ L67), D56 (= D68), L57 (= L69), N83 (= N95), A84 (= A96), A85 (= A97), I86 (= I98), V133 (≠ I147), S135 (= S149), Y148 (= Y162), K152 (= K166), P178 (= P192), G179 (= G193), I181 (≠ T195), T183 (≠ V197), A185 (= A199), V186 (≠ T200)
7tzpG Crystal structure of putataive short-chain dehydrogenase/reductase (fabg) from klebsiella pneumoniae subsp. Pneumoniae ntuh-k2044 in complex with nadh (see paper)
35% identity, 96% coverage: 9:254/255 of query aligns to 6:247/247 of 7tzpG
- binding 1,4-dihydronicotinamide adenine dinucleotide: G15 (= G18), R18 (= R21), G19 (= G22), I20 (≠ L23), D39 (= D42), R40 (≠ L44), C63 (≠ L67), I65 (≠ L69), N91 (= N95), G93 (≠ A97), I94 (= I98), V114 (= V120), Y155 (= Y162), K159 (= K166), I188 (≠ T195), T190 (≠ V197), T193 (= T200)
3ak4A Crystal structure of nadh-dependent quinuclidinone reductase from agrobacterium tumefaciens
36% identity, 97% coverage: 9:255/255 of query aligns to 5:258/258 of 3ak4A
- active site: G18 (= G22), S141 (= S149), L151 (= L159), Y154 (= Y162), K158 (= K166), E199 (= E196)
- binding nicotinamide-adenine-dinucleotide: K17 (≠ R21), G18 (= G22), I19 (≠ L23), D38 (= D42), L39 (≠ V43), V60 (≠ L67), D61 (= D68), V62 (≠ L69), N88 (= N95), A89 (= A96), G90 (≠ A97), T139 (≠ I147), S141 (= S149), Y154 (= Y162), K158 (= K166), G185 (= G193), V187 (≠ T195), T189 (vs. gap), M191 (vs. gap)
7pcsB Structure of the heterotetrameric sdr family member bbscd (see paper)
34% identity, 95% coverage: 9:251/255 of query aligns to 2:241/247 of 7pcsB
- binding nicotinamide-adenine-dinucleotide: G11 (= G18), M16 (≠ L23), D35 (= D42), I36 (≠ V43), I62 (≠ L69), N88 (= N95), G90 (≠ A97), I138 (= I147), S140 (= S149), Y152 (= Y162), K156 (= K166), I185 (≠ T195)
Q9KJF1 (2S)-[(R)-hydroxy(phenyl)methyl]succinyl-CoA dehydrogenase subunit BbsD; (S,R)-2-(alpha-hydroxybenzyl)succinyl-CoA dehydrogenase subunit BbsD; EC 1.1.1.429 from Thauera aromatica (see 2 papers)
34% identity, 95% coverage: 9:251/255 of query aligns to 3:242/248 of Q9KJF1
- S15 (≠ R21) binding
- D36 (= D42) binding
- D62 (= D68) binding
- I63 (≠ L69) binding
- N89 (= N95) binding
- Y153 (= Y162) binding
- K157 (= K166) binding
Sites not aligning to the query:
- 1 modified: Initiator methionine, Removed
5ha5D Crystal structure of an NAD-bound oxidoreductase from brucella ovis
33% identity, 96% coverage: 9:253/255 of query aligns to 4:242/244 of 5ha5D
- active site: G17 (= G22), S142 (= S149), Y155 (= Y162), K159 (= K166)
- binding nicotinamide-adenine-dinucleotide: G13 (= G18), R16 (= R21), G17 (= G22), L18 (= L23), D37 (= D42), I38 (≠ V43), L62 (= L67), D63 (= D68), V64 (≠ L69), N90 (= N95), A91 (= A96), S142 (= S149), Y155 (= Y162), K159 (= K166), G186 (= G193), M188 (≠ T195), S190 (≠ V197)
5itvD Crystal structure of bacillus subtilis bacc dihydroanticapsin 7- dehydrogenase in complex with nadh (see paper)
32% identity, 96% coverage: 9:252/255 of query aligns to 5:224/227 of 5itvD
- active site: G18 (= G22), S141 (= S149), Y154 (= Y162), K158 (= K166)
- binding 1,4-dihydronicotinamide adenine dinucleotide: G14 (= G18), S17 (≠ R21), G18 (= G22), I19 (≠ L23), D38 (= D42), I39 (≠ V43), T61 (≠ L67), D62 (= D68), I63 (≠ L69), N89 (= N95), T139 (≠ I147), S141 (= S149), Y154 (= Y162), K158 (= K166), P184 (= P192), G185 (= G193), I187 (≠ T195)
6vspA Structure of serratia marcescens 2,3-butanediol dehydrogenase mutant q247a (see paper)
35% identity, 95% coverage: 9:251/255 of query aligns to 3:240/251 of 6vspA
- active site: G16 (= G22), S138 (= S149), Y151 (= Y162)
- binding nicotinamide-adenine-dinucleotide: G12 (= G18), N15 (≠ R21), G16 (= G22), M17 (≠ L23), D36 (= D42), W37 (≠ V43), W37 (≠ V43), A38 (≠ L44), I59 (≠ L67), D60 (= D68), V61 (≠ L69), N87 (= N95), A88 (= A96), G89 (≠ A97), V90 (≠ I98), V110 (= V120), T136 (≠ I147), S138 (= S149), Y151 (= Y162), K155 (= K166), P181 (= P192), S182 (≠ G193), L183 (= L194), V184 (≠ T195), T186 (≠ V197), N187 (≠ E198), M188 (≠ A199), T189 (= T200)
H9XP47 Meso-2,3-butanediol dehydrogenase; BDH; meso-2,3-BDH; (R,S)-butane-2,3-diol dehydrogenase; NAD(H)-dependent meso-2,3-BDH; SmBdh; EC 1.1.1.- from Serratia marcescens (see paper)
35% identity, 95% coverage: 9:251/255 of query aligns to 3:240/251 of H9XP47
- N15 (≠ R21) binding
- M17 (≠ L23) binding
- D36 (= D42) binding
- D60 (= D68) binding
- V61 (≠ L69) binding
- N87 (= N95) binding
- S138 (= S149) binding ; binding
- V139 (≠ D150) mutation to Q: Retains 50% of activity with acetoin as substrate; when associated with A-247.
- S140 (≠ T151) binding
- Y151 (= Y162) binding ; binding ; binding
- K155 (= K166) binding
- V184 (≠ T195) binding
- T186 (≠ V197) binding
- RDK 197:199 (≠ HEY 208:210) mutation to SEAAGKPLGYGTET: Mimics longer alpha6 helix. Retains 3% of activity with acetoin as substrate.
Sites not aligning to the query:
- 247 Q→A: Retains 10% of activity with acetoin as substrate. Retains 50% of activity with acetoin as substrate; when associated with Q-139.
6vspB Structure of serratia marcescens 2,3-butanediol dehydrogenase mutant q247a (see paper)
35% identity, 95% coverage: 9:251/255 of query aligns to 5:242/252 of 6vspB
Query Sequence
>BPHYT_RS34945 FitnessBrowser__BFirm:BPHYT_RS34945
MHNDASAALNGRRVLVTGGARGLGAAFVRSLVQAGAQVVFGDVLHEEGRALAASLVEQGH
AVTYVPLDLADPTSIKQFAEQGAAQLGGLDALINNAAITNSGGKFADELSVDTWDAVMNV
NVRGTWLMSTAALPYLRDSGRGSIVNIASDTAMWGAPKLLAYVASKGAVISMTRSLAREF
GAHQVTVNAIAPGLTEVEATAYVPAERHEYYLQGRALTRAQVPDDVTGPVLFLLSDAARF
VTGQLLPVNGGFVMN
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SitesBLAST's Database
SitesBLAST's database includes
(1) SwissProt
entries with experimentally-supported functional features;
and (2) protein structures with bound ligands, from the
BioLip database.
by Morgan Price,
Arkin group
Lawrence Berkeley National Laboratory