SitesBLAST
Comparing BPHYT_RS35475 FitnessBrowser__BFirm:BPHYT_RS35475 to proteins with known functional sites using BLASTp with E ≤ 0.001.
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Found 20 (the maximum) hits to proteins with known functional sites (download)
5b4tA Crystal structure of d-3-hydroxybutyrate dehydrogenase from alcaligenes faecalis complexed with NAD+ and a substrate d-3- hydroxybutyrate (see paper)
41% identity, 96% coverage: 8:254/257 of query aligns to 2:256/260 of 5b4tA
- active site: G15 (= G21), N114 (= N113), S142 (= S141), Y155 (= Y154), K159 (= K158), I200 (= I199)
- binding (3R)-3-hydroxybutanoic acid: Q94 (≠ A93), S142 (= S141), H144 (≠ A143), K152 (≠ V151), Y155 (= Y154), W187 (≠ Y186), Q196 (≠ S195)
- binding nicotinamide-adenine-dinucleotide: G11 (= G17), T13 (≠ G19), G15 (= G21), I16 (= I22), F36 (≠ R42), D63 (= D66), L64 (≠ V67), N90 (= N89), G92 (= G91), L113 (≠ V112), I140 (≠ V139), Y155 (= Y154), K159 (= K158), P185 (= P184), G186 (= G185), W187 (≠ Y186), V188 (≠ T187), T190 (= T189), L192 (= L191), V193 (≠ L192)
3w8dA Crystal structure of d-3-hydroxybutyrate dehydrogenase from alcaligenes faecalis complexed with NAD+ and an inhibitor methylmalonate
41% identity, 96% coverage: 8:254/257 of query aligns to 2:256/260 of 3w8dA
- active site: G15 (= G21), N114 (= N113), S142 (= S141), Y155 (= Y154), K159 (= K158), I200 (= I199)
- binding methylmalonic acid: Q94 (≠ A93), S142 (= S141), H144 (≠ A143), K152 (≠ V151), Y155 (= Y154), W187 (≠ Y186), Q196 (≠ S195)
- binding nicotinamide-adenine-dinucleotide: G11 (= G17), T13 (≠ G19), S14 (= S20), G15 (= G21), I16 (= I22), F36 (≠ R42), A62 (≠ V65), D63 (= D66), L64 (≠ V67), N90 (= N89), A91 (= A90), G92 (= G91), L113 (≠ V112), S142 (= S141), Y155 (= Y154), K159 (= K158), P185 (= P184), G186 (= G185), W187 (≠ Y186), V188 (≠ T187), T190 (= T189), L192 (= L191), V193 (≠ L192)
Sites not aligning to the query:
3vdrA Crystal structure of d-3-hydroxybutyrate dehydrogenase, prepared in the presence of the substrate d-3-hydroxybutyrate and NAD(+) (see paper)
41% identity, 96% coverage: 8:254/257 of query aligns to 2:256/260 of 3vdrA
- active site: G15 (= G21), N114 (= N113), S142 (= S141), Y155 (= Y154), K159 (= K158), I200 (= I199)
- binding (3R)-3-hydroxybutanoic acid: Q94 (≠ A93), H144 (≠ A143), K152 (≠ V151), Y155 (= Y154), W187 (≠ Y186), Q196 (≠ S195)
- binding acetoacetic acid: Q94 (≠ A93), H144 (≠ A143), K152 (≠ V151), Y155 (= Y154), W187 (≠ Y186), Q196 (≠ S195)
- binding nicotinamide-adenine-dinucleotide: G11 (= G17), T13 (≠ G19), I16 (= I22), F36 (≠ R42), D63 (= D66), L64 (≠ V67), N90 (= N89), A91 (= A90), G92 (= G91), L113 (≠ V112), K159 (= K158), G186 (= G185), V188 (≠ T187), T190 (= T189), L192 (= L191), V193 (≠ L192)
- binding 1,4-dihydronicotinamide adenine dinucleotide: G11 (= G17), T13 (≠ G19), I16 (= I22), F36 (≠ R42), D63 (= D66), L64 (≠ V67), N90 (= N89), A91 (= A90), G92 (= G91), L113 (≠ V112), S142 (= S141), Y155 (= Y154), K159 (= K158), G186 (= G185), V188 (≠ T187), T190 (= T189), L192 (= L191), V193 (≠ L192)
Sites not aligning to the query:
3vdqA Crystal structure of alcaligenes faecalis d-3-hydroxybutyrate dehydrogenase in complex with NAD(+) and acetate (see paper)
41% identity, 96% coverage: 8:254/257 of query aligns to 2:256/260 of 3vdqA
- active site: G15 (= G21), N114 (= N113), S142 (= S141), Y155 (= Y154), K159 (= K158), I200 (= I199)
- binding acetate ion: Q94 (≠ A93), H144 (≠ A143), K152 (≠ V151), W187 (≠ Y186), L192 (= L191), Q196 (≠ S195)
- binding nicotinamide-adenine-dinucleotide: G11 (= G17), S14 (= S20), I16 (= I22), F36 (≠ R42), D63 (= D66), L64 (≠ V67), N90 (= N89), A91 (= A90), G92 (= G91), L113 (≠ V112), I140 (≠ V139), S142 (= S141), Y155 (= Y154), K159 (= K158), P185 (= P184), G186 (= G185), W187 (≠ Y186), V188 (≠ T187), T190 (= T189), L192 (= L191), V193 (≠ L192)
2q2qD Structure of d-3-hydroxybutyrate dehydrogenase from pseudomonas putida (see paper)
42% identity, 96% coverage: 7:254/257 of query aligns to 1:251/255 of 2q2qD
- active site: G15 (= G21), S138 (= S141), Y151 (= Y154), K155 (= K158), R196 (≠ I199)
- binding nicotinamide-adenine-dinucleotide: G11 (= G17), T13 (≠ G19), S14 (= S20), G15 (= G21), I16 (= I22), F36 (≠ M40), D59 (≠ C62), L60 (≠ I63), N86 (= N89), G88 (= G91), L109 (≠ V112), I136 (≠ V139), S138 (= S141), Y151 (= Y154), K155 (= K158), P181 (= P184), G182 (= G185), W183 (≠ Y186), V184 (≠ T187), T186 (= T189), L188 (= L191), V189 (≠ L192)
6zzqA Crystal structure of (r)-3-hydroxybutyrate dehydrogenase from acinetobacter baumannii complexed with NAD+ and acetoacetate (see paper)
38% identity, 97% coverage: 5:254/257 of query aligns to 1:256/260 of 6zzqA
- active site: G17 (= G21), S142 (= S141), Y155 (= Y154)
- binding acetoacetic acid: Q94 (≠ A93), S142 (= S141), K152 (≠ V151), Y155 (= Y154), Q196 (≠ S195)
- binding nicotinamide-adenine-dinucleotide: G13 (= G17), S16 (= S20), G17 (= G21), I18 (= I22), D37 (≠ G41), M38 (≠ R42), D63 (= D66), V64 (= V67), N90 (= N89), A91 (= A90), G92 (= G91), M140 (≠ V139), A141 (= A140), S142 (= S141), Y155 (= Y154), K159 (= K158), Y187 (= Y186), V188 (≠ T187), T190 (= T189)
6zzsD Crystal structure of (r)-3-hydroxybutyrate dehydrogenase from acinetobacter baumannii complexed with NAD+ and 3-oxovalerate (see paper)
38% identity, 97% coverage: 5:254/257 of query aligns to 2:257/261 of 6zzsD
- active site: G18 (= G21), S143 (= S141), Y156 (= Y154)
- binding nicotinamide-adenine-dinucleotide: G14 (= G17), S17 (= S20), I19 (= I22), D38 (≠ G41), M39 (≠ R42), D64 (= D66), V65 (= V67), N91 (= N89), A92 (= A90), G93 (= G91), M141 (≠ V139), A142 (= A140), S143 (= S141), Y156 (= Y154), K160 (= K158), P186 (= P184), G187 (= G185), V189 (≠ T187), T191 (= T189), L193 (= L191)
- binding 3-oxidanylidenepentanoic acid: Q95 (≠ A93), S143 (= S141), N145 (≠ A143), K153 (≠ V151), Y156 (= Y154), Q197 (≠ S195)
3sjuA Hedamycin polyketide ketoreductase bound to NADPH (see paper)
43% identity, 95% coverage: 11:254/257 of query aligns to 1:251/255 of 3sjuA
- active site: G11 (= G21), S138 (= S141), Y151 (= Y154), K155 (= K158), Y196 (≠ I199)
- binding nadph dihydro-nicotinamide-adenine-dinucleotide phosphate: G7 (= G17), S10 (= S20), A31 (≠ G41), R32 (= R42), D33 (≠ N43), C56 (≠ V65), D57 (= D66), V58 (= V67), S84 (≠ N89), A85 (= A90), G86 (= G91), I136 (≠ V139), Y151 (= Y154), K155 (= K158), P181 (= P184), G182 (= G185), Y183 (= Y186), V184 (≠ T187), T186 (= T189), M188 (≠ L191)
P16544 Putative ketoacyl reductase; EC 1.3.1.- from Streptomyces coelicolor (strain ATCC BAA-471 / A3(2) / M145) (see 2 papers)
40% identity, 94% coverage: 13:254/257 of query aligns to 9:257/261 of P16544
- 11:39 (vs. 15:43, 45% identical) binding
- D63 (≠ I63) binding
- K161 (= K158) binding
1w4zA Structure of actinorhodin polyketide (actiii) reductase (see paper)
40% identity, 94% coverage: 13:254/257 of query aligns to 7:255/259 of 1w4zA
- active site: G15 (= G21), N112 (= N113), S142 (= S141), Y155 (= Y154), K159 (= K158), Y200 (≠ I199)
- binding nadp nicotinamide-adenine-dinucleotide phosphate: G11 (= G17), T13 (≠ G19), S14 (= S20), G15 (= G21), I16 (= I22), R36 (= R42), G37 (≠ N43), D61 (≠ I63), V62 (≠ S64), N88 (= N89), G90 (= G91), S142 (= S141), Y155 (= Y154), K159 (= K158), P185 (= P184), G186 (= G185), V188 (≠ T187), T190 (= T189)
2rh4A Actinorhodin ketoreductase, actkr, with NADPH and inhibitor emodin (see paper)
40% identity, 94% coverage: 13:254/257 of query aligns to 5:253/257 of 2rh4A
- active site: G13 (= G21), N110 (= N113), S140 (= S141), Y153 (= Y154), K157 (= K158), Y198 (≠ I199)
- binding 3-methyl-1,6,8-trihydroxyanthraquinone: T141 (= T142), Q145 (≠ I146), V147 (≠ Y148), Y153 (= Y154), F185 (≠ Y186)
- binding nadph dihydro-nicotinamide-adenine-dinucleotide phosphate: G9 (= G17), T11 (≠ G19), S12 (= S20), G13 (= G21), I14 (= I22), A33 (≠ G41), R34 (= R42), G35 (≠ N43), C58 (= C62), D59 (≠ I63), V60 (≠ S64), N86 (= N89), G88 (= G91), S140 (= S141), Y153 (= Y154), K157 (= K158), P183 (= P184), G184 (= G185), V186 (≠ T187), T188 (= T189), M190 (≠ L191)
Sites not aligning to the query:
1xr3A Actinorhodin polyketide ketoreductase with NADP and the inhibitor isoniazid bound (see paper)
40% identity, 94% coverage: 13:254/257 of query aligns to 4:252/256 of 1xr3A
- active site: G12 (= G21), N109 (= N113), S139 (= S141), Y152 (= Y154), K156 (= K158), Y197 (≠ I199)
- binding 4-(diazenylcarbonyl)pyridine: T140 (= T142), G141 (≠ A143), V146 (≠ Y148)
- binding nadp nicotinamide-adenine-dinucleotide phosphate: G8 (= G17), T10 (≠ G19), S11 (= S20), G12 (= G21), I13 (= I22), A32 (≠ G41), R33 (= R42), G34 (≠ N43), C57 (= C62), D58 (≠ I63), V59 (≠ S64), N85 (= N89), A86 (= A90), G87 (= G91), S139 (= S141), Y152 (= Y154), K156 (= K158), G183 (= G185), V185 (≠ T187), T187 (= T189), P188 (≠ E190)
2rh4B Actinorhodin ketoreductase, actkr, with NADPH and inhibitor emodin (see paper)
40% identity, 94% coverage: 13:254/257 of query aligns to 16:264/268 of 2rh4B
- active site: G24 (= G21), N121 (= N113), S151 (= S141), Y164 (= Y154), K168 (= K158), Y209 (≠ I199)
- binding nadph dihydro-nicotinamide-adenine-dinucleotide phosphate: G20 (= G17), T22 (≠ G19), S23 (= S20), I25 (= I22), A44 (≠ G41), R45 (= R42), G46 (≠ N43), C69 (= C62), D70 (≠ I63), V71 (≠ S64), N97 (= N89), S151 (= S141), Y164 (= Y154), K168 (= K158), G195 (= G185), V197 (≠ T187), T199 (= T189), M201 (≠ L191)
6d9yB Crystal structure of a short chain dehydrogenase/reductase sdr from burkholderia phymatum with partially occupied NAD
39% identity, 96% coverage: 8:254/257 of query aligns to 7:247/251 of 6d9yB
- active site: G20 (= G21), S145 (= S141), Y158 (= Y154)
- binding nicotinamide-adenine-dinucleotide: G16 (= G17), R19 (≠ S20), G20 (= G21), D40 (≠ G41), L41 (≠ R42), V64 (= V65), D65 (= D66), Q66 (≠ V67), A93 (= A90), S145 (= S141), Y158 (= Y154), K162 (= K158), P188 (= P184), A189 (≠ G185), A190 (≠ Y186), A191 (≠ T187), T193 (= T189)
2ztlA Closed conformation of d-3-hydroxybutyrate dehydrogenase complexed with NAD+ and l-3-hydroxybutyrate (see paper)
40% identity, 96% coverage: 8:254/257 of query aligns to 2:256/260 of 2ztlA
- active site: G15 (= G21), N114 (= N113), S142 (= S141), Y155 (= Y154), K159 (= K158), L200 (≠ I199)
- binding (3s)-3-hydroxybutanoic acid: Q94 (≠ A93), S142 (= S141), H144 (≠ A143), K152 (≠ V151), Y155 (= Y154), Q196 (≠ S195)
- binding nicotinamide-adenine-dinucleotide: G11 (= G17), G15 (= G21), I16 (= I22), F36 (≠ R42), L64 (≠ I63), N90 (= N89), A91 (= A90), G92 (= G91), L113 (≠ V112), Y155 (= Y154), K159 (= K158), P185 (= P184), W187 (≠ Y186), V188 (≠ T187), T190 (= T189), V193 (≠ L192)
1wmbA Crystal structure of NAD dependent d-3-hydroxybutylate dehydrogenase (see paper)
40% identity, 96% coverage: 8:254/257 of query aligns to 2:256/260 of 1wmbA
Sites not aligning to the query:
5yssB Crystal structure of aminocaproic acid cyclase in complex with NAD (+) (see paper)
38% identity, 97% coverage: 7:256/257 of query aligns to 1:253/255 of 5yssB
- binding nicotinamide-adenine-dinucleotide: G11 (= G17), T13 (≠ G19), S14 (= S20), G15 (= G21), I16 (= I22), G35 (= G41), F36 (≠ R42), L60 (≠ V67), N86 (= N89), G88 (= G91), I89 (≠ Q92), A137 (= A140), Y151 (= Y154), K155 (= K158), P181 (= P184), G182 (= G185), V184 (≠ T187), T186 (= T189)
3op4A Crystal structure of putative 3-ketoacyl-(acyl-carrier-protein) reductase from vibrio cholerae o1 biovar eltor str. N16961 in complex with NADP+ (see paper)
38% identity, 97% coverage: 8:257/257 of query aligns to 6:246/247 of 3op4A
- binding nadp nicotinamide-adenine-dinucleotide phosphate: G15 (= G17), S17 (≠ G19), R18 (≠ S20), I20 (= I22), T40 (≠ A44), N62 (≠ D66), V63 (= V67), N89 (= N89), A90 (= A90), I92 (≠ Q92), V139 (= V139), S141 (= S141), Y154 (= Y154), K158 (= K158), P184 (= P184), G185 (= G185), I187 (≠ T187), T189 (= T189), M191 (≠ L191)
4nbuB Crystal structure of fabg from bacillus sp (see paper)
37% identity, 98% coverage: 6:257/257 of query aligns to 3:244/244 of 4nbuB
- active site: G18 (= G21), N111 (= N113), S139 (= S141), Q149 (≠ V151), Y152 (= Y154), K156 (= K158)
- binding acetoacetyl-coenzyme a: D93 (≠ A95), K98 (≠ H100), S139 (= S141), N146 (≠ Y148), V147 (≠ A149), Q149 (≠ V151), Y152 (= Y154), F184 (≠ Y186), M189 (≠ L191), K200 (= K202)
- binding 1,4-dihydronicotinamide adenine dinucleotide: G14 (= G17), N17 (≠ S20), G18 (= G21), I19 (= I22), D38 (≠ G41), F39 (≠ R42), V59 (= V65), D60 (= D66), V61 (= V67), N87 (= N89), A88 (= A90), G89 (= G91), I90 (≠ Q92), T137 (≠ V139), S139 (= S141), Y152 (= Y154), K156 (= K158), P182 (= P184), F184 (≠ Y186), T185 (= T187), T187 (= T189), M189 (≠ L191)
5vmlA Crystal structure of acetoacetyl-coa reductase from burkholderia pseudomallei 1710b with bound NADP
39% identity, 94% coverage: 13:254/257 of query aligns to 5:241/245 of 5vmlA
- active site: G13 (= G21), N111 (= N113), S139 (= S141), Y152 (= Y154), K156 (= K158)
- binding nadp nicotinamide-adenine-dinucleotide phosphate: G9 (= G17), G12 (≠ S20), G13 (= G21), I14 (= I22), C33 (≠ M40), G34 (= G41), R39 (= R46), G59 (≠ V65), N60 (≠ D66), V61 (= V67), N87 (= N89), G89 (= G91), I90 (≠ Q92), S139 (= S141), Y152 (= Y154), K156 (= K158), P182 (= P184), G183 (= G185), I185 (≠ T187)
Query Sequence
>BPHYT_RS35475 FitnessBrowser__BFirm:BPHYT_RS35475
MNTFSSTLAGQHAVVTGGGSGIGAAVAEALLRAGARVTLMGRNAERLDVQRAKCRALGDV
ACISVDVTQEDSVASAFAEAGAIDILVNNAGQAQAAPFTHTDMALWQRMLDVNLTGVFLG
TRAALPGMLERGHGRIVNVASTAGQIGYAYVAAYCAAKHGVIGLTRSLALEVATKGVTVN
AVCPGYTETELLHASLEQITSKTSRTEQQARETLLRSNPQHRFVSPEQVANAVLWLCQPG
SDAVTGQSISISGGEVM
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SitesBLAST's Database
SitesBLAST's database includes
(1) SwissProt
entries with experimentally-supported functional features;
and (2) protein structures with bound ligands, from the
BioLip database.
by Morgan Price,
Arkin group
Lawrence Berkeley National Laboratory