SitesBLAST

SitesBLAST – Find functional sites

SitesBLAST

Comparing BWI76_RS00615 FitnessBrowser__Koxy:BWI76_RS00615 to proteins with known functional sites using BLASTp with E ≤ 0.001.

Or try Sites on a Tree, PaperBLAST, Conserved Domains, or compare to all protein structures

Found 20 (the maximum) hits to proteins with known functional sites (download)

P07464 Galactoside O-acetyltransferase; GAT; Acetyl-CoA:galactoside 6-O-acetyltransferase; Thiogalactoside acetyltransferase; Thiogalactoside transacetylase; EC 2.3.1.18 from Escherichia coli (strain K12) (see 3 papers)
57% identity, 97% coverage: 1:191/196 of query aligns to 3:193/203 of P07464

query
sites
P07464

M

M

D

P

M

M

K

T

A

E

R

R

M

I

N

R

S

A

G

G

E

K

L

L

Y

F

L

T

D
|
D

S

M

G

C

Y

E

G

G

L

L

P

P

E

E

Q

K

R

R

L

L

M

R

G

G

K

K

A

T

R

L

A

M

Y

Y

E

E

Y

F

N

N

H

H

S

S

H

H

P

P

F

S

N

E

Q

V

E

E

L

K

R

R

D

E

D

S

I

L

I

I

R

K

R

E

M

M

F

F

A

A

S

T

V

V

G

G

K

E

A

N

C

A

W

W

I

V

E

E

P

P

I

V

N

Y

I

F

A
x
S

Y

Y

G

G

T

S

H

N

T

I

T

H

I

I

G

G

D

R

N

N

F

F

Y

Y

A

A

N
|
N

F

F

N

N

L

L

T

T

L

I

V

V

D

D

D
|
D

A

Y

D

T

I

V

I

T

I

I

G

G

N

D

N

N

V

V

M

L

F

I

A

A

P

P

N

N

V

V

T

T

I

L

S

S

T

V

A

T

G

G

H
|
H

P

P

V

V

H

H

P

H

D

E

L

L

R

R

H

K

T

N

Q

G

Q

E

Q

M

F

Y

S

S

Q

F

P

P

V

I

I

T

I

I

E

G

D

N

G

N

V

V

W

W

L

I

G

G

T
x
S

G

H

V

V

I

V

I

I

N

N

P

P

G

G

V

V

T

T

I

I

G

G

K

D

N

N

S

S

V

V

I

I

G

G

A
|
A

G

G

S

S

V

I

V

V

N
x
T

R
x
K

S

D

I

I

P

P

A

P

N

N

V

V

A

A

G

G

V

V

P

P

C

C

R
|
R

I

V

L

I

R
|
R

E

E

I

I

S

N

D

D

E

R

D

D

K

K

N

H

K

Y

Y

Y

D17 (= D15) binding
S71 (≠ A69) binding
N85 (= N83) binding in other chain; binding in other chain
D93 (= D91) binding
H115 (= H113) mutation to A: Results in an 1800-fold decrease in catalytic activity.
S142 (≠ T140) binding in other chain
A160 (= A158) binding in other chain
TK 165:166 (≠ NR 163:164) binding
R180 (= R178) binding
R183 (= R181) binding in other chain

Sites not aligning to the query:

1 modified: Initiator methionine, Removed; Partial

1krvA Galactoside acetyltransferase in complex with coa and pnp-beta-gal (see paper)
57% identity, 97% coverage: 1:191/196 of query aligns to 2:192/201 of 1krvA

query
sites
1krvA

M

M

D

P

M

M

K

T

A

E

R

R

M

I

N

R

S

A

G

G

E

K

L

L

Y

F

L

T

D
|
D

S

M

G

C

Y

E

G

G

L

L

P

P

E

E

Q

K

R
|
R

L

L

M

R

G

G

K

K

A

T

R

L

A

M

Y

Y

E

E

Y

F

N

N

H

H

S

S

H

H

P

P

F

S

N

E

Q

V

E

E

L

K

R

R

D

E

D

S

I

L

I

I

R

K

R

E

M

M

F

F

A

A

S

T

V

V

G

G

K

E

A

N

C

A

W

W

I

V

E

E

P

P

I

V

N

Y

I

F

A
x
S

Y

Y

G

G

T

S

H

N

T

I

T

H

I

I

G

G

D

R

N

N

F

F

Y
|
Y

A

A

N
|
N

F

F

N

N

L

L

T

T

L

I

V
|
V

D

D

D
|
D

A

Y

D

T

I

V

I

T

I

I

G

G

N

D

N

N

V

V

M

L

F

I

A
|
A

P

P

N

N

V

V

T

T

I

L

S
|
S

T

V

A
x
T

G

G

H

H

P

P

V

V

H

H

P

H

D

E

L

L

R

R

H

K

T

N

Q

G

Q

E

Q
x
M

F

Y

S

S

Q

F

P

P

V

I

I

T

I

I

E

G

D

N

G

N

V

V

W
|
W

L

I

G
|
G

T
x
S

G

H

V

V

I

V

I

I

N
|
N

P

P

G

G

V

V

T

T

I

I

G

G

K

D

N

N

S

S

V

V

I

I

G

G

A
|
A

G

G

S

S

V

I

V

V

N

T

R

K

S

D

I

I

P

P

A

P

N

N

V

V

A

A

G

G

V

V

P
|
P

C

C

R
|
R

I

V

L

I

R
|
R

E

E

I

I

S

N

D

D

E

R

D

D

K

K

N

H

K

Y

Y

Y

binding 4-nitrophenyl beta-D-galactopyranoside: D16 (= D15), R25 (= R24), S70 (≠ A69), Y82 (= Y81), N84 (= N83), V90 (= V89), D92 (= D91), M126 (≠ Q125)
binding coenzyme a: A104 (= A103), S110 (= S109), T112 (≠ A111), W138 (= W137), G140 (= G139), S141 (≠ T140), N146 (= N145), A159 (= A158), P177 (= P176), R179 (= R178), R182 (= R181)

1kruA Galactoside acetyltransferase in complex with iptg and coenzyme a (see paper)
57% identity, 97% coverage: 1:191/196 of query aligns to 2:192/201 of 1kruA

query
sites
1kruA

M

M

D

P

M

M

K

T

A

E

R

R

M

I

N

R

S

A

G

G

E

K

L

L

Y

F

L

T

D
|
D

S

M

G

C

Y

E

G

G

L

L

P
|
P

E

E

Q

K

R
|
R

L

L

M

R

G

G

K

K

A

T

R

L

A

M

Y

Y

E

E

Y

F

N

N

H

H

S

S

H

H

P

P

F

S

N

E

Q

V

E

E

L

K

R

R

D

E

D

S

I

L

I

I

R

K

R

E

M

M

F

F

A

A

S

T

V

V

G

G

K

E

A

N

C

A

W
|
W

I

V

E

E

P

P

I

V

N

Y

I

F

A
x
S

Y

Y

G

G

T

S

H

N

T

I

T

H

I

I

G

G

D

R

N

N

F

F

Y
|
Y

A

A

N
|
N

F

F

N

N

L

L

T

T

L

I

V
|
V

D

D

D
|
D

A

Y

D

T

I

V

I

T

I

I

G

G

N

D

N

N

V

V

M
x
L

F

I

A

A

P

P

N

N

V

V

T

T

I

L

S

S

T

V

A

T

G

G

H
|
H

P

P

V

V

H

H

P

H

D

E

L

L

R

R

H

K

T

N

Q

G

Q

E

Q
x
M

F

Y

S

S

Q

F

P

P

V

I

I

T

I

I

E

G

D

N

G

N

V

V

W
|
W

L

I

G
|
G

T
x
S

G

H

V

V

I

V

I

I

N

N

P

P

G

G

V

V

T

T

I

I

G

G

K

D

N

N

S

S

V

V

I

I

G
|
G

A
|
A

G

G

S

S

V

I

V

V

N

T

R

K

S

D

I

I

P

P

A

P

N

N

V

V

A

A

G

G

V

V

P
|
P

C

C

R

R

I

V

L

I

R
|
R

E

E

I

I

S

N

D

D

E

R

D

D

K

K

N

H

K

Y

Y

Y

binding coenzyme a: H114 (= H113), W138 (= W137), G140 (= G139), S141 (≠ T140), G158 (= G157), A159 (= A158), P177 (= P176), R182 (= R181)
binding 1-methylethyl 1-thio-beta-D-galactopyranoside: D16 (= D15), P22 (= P21), R25 (= R24), W62 (= W61), S70 (≠ A69), Y82 (= Y81), Y82 (= Y81), N84 (= N83), V90 (= V89), D92 (= D91), L102 (≠ M101), H114 (= H113), M126 (≠ Q125)

1krrA Galactoside acetyltransferase in complex with acetyl-coenzyme a (see paper)
57% identity, 97% coverage: 1:191/196 of query aligns to 2:192/200 of 1krrA

query
sites
1krrA

M

M

D

P

M

M

K

T

A

E

R

R

M

I

N

R

S

A

G

G

E

K

L

L

Y

F

L

T

D

D

S

M

G

C

Y

E

G

G

L

L

P

P

E

E

Q

K

R

R

L

L

M

R

G

G

K

K

A

T

R

L

A

M

Y

Y

E

E

Y

F

N

N

H

H

S

S

H

H

P

P

F

S

N

E

Q

V

E

E

L

K

R

R

D

E

D

S

I

L

I

I

R

K

R

E

M

M

F

F

A

A

S

T

V

V

G

G

K

E

A

N

C

A

W

W

I

V

E

E

P

P

I

V

N

Y

I

F

A

S

Y

Y

G

G

T

S

H

N

T

I

T

H

I

I

G

G

D

R

N

N

F

F

Y

Y

A

A

N
|
N

F

F

N

N

L

L

T

T

L

I

V

V

D

D

A

Y

D

T

I

V

I

T

I

I

G

G

N

D

N

N

V

V

M

L

F
x
I

A
|
A

P
|
P

N

N

V

V

T

T

I

L

S

S

T

V

A
x
T

G

G

H
|
H

P

P

V

V

H

H

P

H

D

E

L

L

R

R

H

K

T

N

Q

G

Q

E

Q

M

F

Y

S

S

Q

F

P

P

V

I

I

T

I

I

E

G

D

N

G

N

V

V

W

W

L

I

G
|
G

T
x
S

G

H

V

V

I

V

I

I

N
|
N

P

P

G

G

V

V

T

T

I

I

G

G

K

D

N

N

S

S

V

V

I

I

G
|
G

A
|
A

G

G

S

S

V

I

V

V

N

T

R

K

S

D

I

I

P

P

A

P

N

N

V

V

A

A

A
|
A

G

G

V

V

P
|
P

C

C

R

R

I

V

L

I

R
|
R

E

E

I

I

S

N

D

D

E

R

D

D

K

K

N

H

K

Y

Y

Y

binding acetyl coenzyme *a: N84 (= N83), I103 (≠ F102), A104 (= A103), P105 (= P104), T112 (≠ A111), H114 (= H113), G140 (= G139), S141 (≠ T140), N146 (= N145), G158 (= G157), A159 (= A158), A174 (= A173), P177 (= P176), R182 (= R181)

4isxA The crystal structure of maltose o-acetyltransferase from clostridium difficile 630 in complex with acetyl-coa
44% identity, 94% coverage: 1:185/196 of query aligns to 2:186/186 of 4isxA

query
sites
4isxA

M

M

D

T

M

E

K

K

A

E

R

K

M

M

N

L

S

S

G

G

E

K

L

G

Y

Y

L

Y

D

A

S

N

G

D

Y

E

G

L

L

L

P

V

E

K

Q

E

R

R

L

E

M

Y

G

C

K

K

A

K

R

L

A

T

Y

R

E

L

Y

F

N

N

H

N

S

T

H

L

P

E

F

D

N

E

Q

Y

E

E

L

K

R

R

D

E

D

D

I

I

I

L

R

R

R

Q

M

L

F

F

A

G

S

S

V

V

G

G

K

K

A

Q

C

I

W

N

I

V

E

E

P

Q

P

N

I

I

N

R

I

C

A

D

Y

Y

G

G

T

Y

H

N

T

I

T

H

I

V

G

G

D

E

N

N

F

F

Y

F

A

A

N
|
N

F

Y

N

D

L

C

T

I

L

F

V

L

D

D

D

V

A

C

D

K

I

I

I

E

I

I

G

G

N

D

N

N

V

V

M

M

F

L

A
|
A

P

P

N

N

V

V

T

Q

I

I

S

Y

T

T

A

A

G

Y

H

H

P

P

V

I

H

D

P

A

D

Q

L

L

R

R

H

N

T

S

Q

G

Q

I

Q

E

F

Y

S

G

Q

S

P

P

V

V

I

K

I

I

E

G

D

D

G

N

V

V

W
|
W

L

I

G
|
G

T
x
G

G

G

V

V

I

I

N

T

P

P

G

G

V

I

T

T

I

I

G

G

K

D

N

N

S

V

V

V

I

I

G

G

A
|
A

G

G

S

S

V

V

N

T

R

K

S

D

I

I

P

P

A

P

N

N

V

T

V

V

A

A

A
x
V

G

G

V

N

P

P

C

C

R

R

I

V

L

I

R
x
K

E

K

I

I

S

E

D

E

binding acetyl coenzyme *a: N84 (= N83), A104 (= A103), W138 (= W137), G140 (= G139), G141 (≠ T140), A159 (= A158), V174 (≠ A173), K182 (≠ R181)

3igjC Crystal structure of maltose o-acetyltransferase complexed with acetyl coenzyme a from bacillus anthracis
38% identity, 93% coverage: 4:185/196 of query aligns to 6:188/188 of 3igjC

query
sites
3igjC

K

K

A

D

R

K

M

M

N

L

S

A

G

G

E

E

L

M

Y

Y

L

I

D

A

S

D

G

D

Y

E

G

E

L

L

P

V

E

A

Q

D

R

R

L

V

M

E

G

A

K

K

A

R

R

L

A

T

Y

R

E

L

Y

Y

N

N

H

E

S

A

H

V

P

E

F

T

N

G

Q

D

E

E

L

R

R

R

D

F

D

T

I

L

R

N

R

Q

M

L

F

L

A

G

S

S

V

S

-

A

-

D

G

G

K

K

A

A

C

-

W

Q

I

I

E

N

P

P

P

D

I

F

N

R

I

C

A

D

Y

Y

G

G

T

Y

H

N

T

I

T

H

I

V

G

G

D

K

N

S

F

F

Y
x
F

A

A

N

N

F

F

N

N

L

C

T

V

L

I

V

L

D

D

D

V

A

C

D

E

I

V

I

R

I

I

G

G

N

D

N

H

V

C

M

M

F

F

A
|
A

P

P

N

G

V

V

T

H

I

I

S
x
Y

T

T

A
|
A

G

T

H
|
H

P

P

V

L

H

H

P

P

D

V

L

E

R

R

H

N

T

S

Q

G

Q

K

Q

E

F

Y

S

G

Q

K

P

P

V

V

I

K

I

I

E

G

D

N

G

N

V

V

W

W

L

V

G
|
G

T

G

G

G

V

A

I

I

N
|
N

P

P

G

G

V

V

T

S

I

I

G

G

K

D

N

N

S

A

V

V

I

I

G
x
A

A
x
S

G

G

S

A

V

V

N
x
T

R

K

S

D

I

V

P

P

A

N

N

N

V

V

A

V

A

G

G

G

V
x
N

P

P

C

A

R

K

I

V

L

I

R

K

E

T

I

I

S

E

D

E

binding acetyl coenzyme *a: F84 (≠ Y81), A106 (= A103), Y112 (≠ S109), A114 (= A111), H116 (= H113), G142 (= G139), N148 (= N145), A160 (≠ G157), S161 (≠ A158), T166 (≠ N163), N178 (≠ V175)

3nz2C Crystal structure of hexapeptide-repeat containing-acetyltransferase vca0836 complexed with acetyl co enzyme a from vibrio cholerae o1 biovar eltor
33% identity, 94% coverage: 1:185/196 of query aligns to 1:183/183 of 3nz2C

query
sites
3nz2C

M

M

D

S

M

E

K

L

A

E

R

K

M

M

N

L

S

K

G

G

E

E

L

H

Y

F

L

D

D

G

S

A

G

S

Y

A

G

E

L

I

P

E

E

A

Q

L

R

R

L

S

M

Q

G

A

K

G

A

R

R

L

A

K

Y

L

E

E

Y

I

N

N

H

Q

S

S

H

-

P

-

F

L

N

D

Q

E

E

A

L

E

R

R

D

Y

D

A

I

L

I

Q

R

R

R

E

M

L

F

F

A

G

S

H

V

L

G

G

K

H

A

K

C

S

W

C

I

V

E

Q

P

P

I

F

N

H

I

C

A

E

Y

F

G

G

T

K

H

T

T

I

T

R

I

I

G

G

D

D

N

H

F

T

Y

F

A

I

N

N

F

M

N

N

L

V

T

V

L

M

V

L

D

D

D

G

A

A

D

P

I

I

I

T

I

I

G

G

N

D

N

H

V

V

M

L

F

I

A

G

P

P

N

S

V

T

T

Q

I

F

S
x
Y

T

T

A

A

G

S

H
|
H

P

S

V

L

H

D

P

Y

D

R

L

R

R

R

H

Q

T

A

Q

W

Q

E

Q

T

F

I

S

C

Q

K

P

P

V

I

I

V

I

I

E

E

D

D

G

D

V

V

W

W

L

I

G
|
G

T

G

G

N

V

V

I

V

I

I

N

N

P

Q

G

G

V

V

T

T

I

I

G

G

K

A

N

R

S

S

V

V

I

V

G
x
A

A
|
A

G
x
N

S

S

V

V

N
|
N

R

Q

S

D

I

V

P

P

A

P

N

D

V

T

V

L

A

V

A
x
G

G

G

V
x
T

P
|
P

C

A

R

R

I

I

L
|
L

R
|
R

E

S

I

L

S

K

D

D

binding acetyl coenzyme *a: Y107 (≠ S109), H111 (= H113), G137 (= G139), A155 (≠ G157), A156 (= A158), N161 (= N163), G171 (≠ A173), T173 (≠ V175), P174 (= P176), L178 (= L180), R179 (= R181)
binding magnesium ion: N157 (≠ G159), T173 (≠ V175)

5u2kA Crystal structure of galactoside o-acetyltransferase complex with coa (h3 space group)
35% identity, 95% coverage: 1:186/196 of query aligns to 1:187/190 of 5u2kA

query
sites
5u2kA

M

M

D

T

M

E

K

K

A

E

R

K

M

M

N

-

S

L

G

A

E

E

L

K

Y

W

L

Y

D

D

S

A

G

N

Y

F

G

D

-

Q

-

Y

L

L

P

I

E

N

Q

E

R

R

L

A

M

R

G

A

K

K

A

D

R

I

A

C

Y

F

E

E

Y

L

N

N

H

H

S

T

H

R

P

P

F

S

N

A

Q

T

E

N

L

K

R

R

D

K

D

E

I

L

I

I

R

D

R

Q

M

L

F

F

A

Q

S

T

V

T

G

T

K

D

A

N

C

V

W

S

I

I

E

S

P

I

P

P

I

F

N

D

I

T

A

D

Y

Y

G

G

T

W

H

N

T

V

T

K

I

L

G

G

D

K

N

N

F

V

Y

Y

A

V

N

N

F

T

N

N

L

C

T

Y

L

F

V
x
M

D

D

D

G

A

G

D

Q

I

I

I

T

I

I

G

G

N

D

N

N

V

V

M

F

F

I

A

G

P

P

N

N

V

C

T

G

I

F

S
x
Y

T

T

A
|
A

G

T

H
|
H

P

P

V

L

H

N

P

F

D

H

L

H

R

R

H

N

T

E

Q

G

Q

F

Q

E

F

K

S

A

Q

G

P

P

V

I

I

H

I

I

E

G

D

S

G

N

V

T

W

W

L

F

G

G

T

G

G

H

V

V

I

A

I

V

N
x
L

P

P

G

G

V

V

T

T

I

I

G

G

K

E

N

G

S

S

V

V

I

I

G

G

A

A

G

G

S

S

V

V

N
x
T

R
x
K

S

D

I

I

P

P

A

P

N

H

V

S

V

L

A

A

A

V

G

G

V

N

P

P

C

C

R

K

I

V

L

V

R

R

E

K

I

I

S

D

D

N

E

D

binding coenzyme a: M90 (≠ V89), Y110 (≠ S109), A112 (= A111), H114 (= H113), L146 (≠ N145), T164 (≠ N163), K165 (≠ R164)

3ectA Crystal structure of the hexapeptide-repeat containing- acetyltransferase vca0836 from vibrio cholerae
34% identity, 83% coverage: 23:185/196 of query aligns to 14:176/176 of 3ectA

query
sites
3ectA

Q

E

R

A

L

L

M

R

G

S

K

Q

A

A

R

G

A

R

Y

L

E

K

Y

L

N

E

H

I

S

N

H

Q

P

S

F

L

N

D

Q

E

E

A

L

E

R

R

D

Y

D

A

I

L

I

Q

R

R

R

E

M

L

F

F

A

G

S

H

V

L

G

G

K

H

A

K

C

S

W

C

I

V

E

Q

P

P

I

F

N

H

I

C

A

E

Y

F

G

G

T

K

H

T

T

I

T

R

I

I

G

G

D

D

N

H

F

T

Y

F

A

I

N

N

F

M

N

N

L

V

T

V

L

M

V

L

D

D

D

G

A

A

D

P

I

I

I

T

I

I

G

G

N

D

N

H

V

V

M

L

F

I

A

G

P

P

N

S

V

T

T

Q

I

F

S

Y

T

T

A

A

G

S

H

H

P

S

V

L

H

D

P

Y

D

R

L

R

R

R

H

Q

T

A

Q

W

Q

E

Q

T

F

I

S

C

Q

K

P

P

V

I

I

V

I

I

E

E

D

D

G

D

V

V

W

W

L

I

G

G

T

G

G

N

V

V

I

V

I

I

N

N

P

Q

G
|
G

V

V

T

T

I

I

G

G

K

A

N

R

S

S

V

V

I

V

G

A

A

A

G

N

S

S

V

V

N

N

R

Q

S
x
D

I

V

P

P

A

P

N

D

V

T

V

L

A

V

A

G

G

G

V

T

P

P

C

A

R

R

I

I

L

L

R

R

E

S

I

L

S

K

D

D

binding calcium ion: G138 (= G147), D156 (≠ S165)

3nz2J Crystal structure of hexapeptide-repeat containing-acetyltransferase vca0836 complexed with acetyl co enzyme a from vibrio cholerae o1 biovar eltor
33% identity, 94% coverage: 1:184/196 of query aligns to 4:185/185 of 3nz2J

query
sites
3nz2J

M

M

D

S

M

E

K

L

A

E

R

K

M

M

N

L

S

K

G

G

E

E

L

H

Y

F

L

D

D

G

S

A

G

S

Y

A

G

E

L

I

P

E

E

A

Q

L

R

R

L

S

M

Q

G

A

K

G

A

R

R

L

A

K

Y

L

E

E

Y

I

N

N

H

Q

S

S

H

-

P

-

F

L

N

D

Q

E

E

A

L

E

R

R

D

Y

D

A

I

L

I

Q

R

R

R

E

M

L

F

F

A

G

S

H

V

L

G

G

K

H

A

K

C

S

W

C

I

V

E

Q

P

P

I

F

N

H

I

C

A

E

Y

F

G

G

T

K

H

T

T

I

T

R

I

I

G

G

D

D

N

H

F

T

Y

F

A

I

N

N

F

M

N

N

L

V

T

V

L

M

V

L

D

D

D

G

A

A

D

P

I

I

I

T

I

I

G

G

N

D

N

H

V

V

M

L

F

I

A
x
G

P

P

N

S

V

T

T

Q

I

F

S
x
Y

T

T

A

A

G

S

H
|
H

P

S

V

L

H

D

P

Y

D

R

L

R

R

R

H

Q

T

A

Q

W

Q

E

Q

T

F

I

S

C

Q

K

P

P

V

I

I

V

I

I

E

E

D

D

G

D

V

V

W
|
W

L

I

G
|
G

T

G

G

N

V

V

I

V

I

I

N

N

P

Q

G

G

V

V

T

T

I

I

G

G

K

A

N

R

S

S

V

V

I

V

G
x
A

A
|
A

G

N

S

S

V

V

N
|
N

R

Q

S

D

I

V

P

P

A

P

N

D

V

T

V

L

A

V

A
x
G

G

G

V
x
T

P
|
P

C

A

R

R

I

I

L

L

R
|
R

E

S

I

L

S

K

binding acetyl coenzyme *a: G104 (≠ A103), Y110 (≠ S109), H114 (= H113), W138 (= W137), G140 (= G139), A158 (≠ G157), A159 (= A158), N164 (= N163), G174 (≠ A173), T176 (≠ V175), P177 (= P176), R182 (= R181)

A1ADJ6 Polysialic acid O-acetyltransferase; Capsule O-acetyl transferase; EC 2.3.1.136 from Escherichia coli O1:K1 / APEC (see paper)
33% identity, 59% coverage: 68:182/196 of query aligns to 169:279/307 of A1ADJ6

query
sites
A1ADJ6

I

V

A

I

Y

I

G

G

T

R

H

R

T

T

I

I

G

G

D

A

N

G

F

F

Y

E

A

V

N

-

F

-

N

-

L

-

T

-

L

V

V

T

D

D

D

K

A

C

D

N

I

V

I

T

I

I

G

G

N

H

N

D

V

C

M

M

F

I

A

A

P

R

N

D

V

V

T

I

I

L

-

R

S

A

T

S

A

D

G

G

H
|
H

P

P

V

I

H

F

P

D

D

I

L

H

R

S

H

K

T

K

Q

R

Q

I

Q

N

F

W

S

A

Q

K

P

D

V

I

I

I

E

S

D

S

G

Y

V

V

W
|
W

L

V

G

G

T

R

G

N

V

V

I

S

I

I

N

M

P

K

G

G

V

V

T

S

I

V

G

G

K

S

N

G

S

S

V

V

I

I

G

G

A

Y

G

G

S

S

V

I

V

V

N

T

R

K

S

D

I

V

P

P

A

S

N

M

V

C

V

A

A

A

G

G

V

N

P

P

C

A

R

K

I

I

L

I

R

K

E

R

H210 (= H113) mutation to A: Loss of activity.
W234 (= W137) mutation to A: Loss of activity.

3gvdI Crystal structure of serine acetyltransferase cyse from yersinia pestis
29% identity, 62% coverage: 59:180/196 of query aligns to 132:247/272 of 3gvdI

query
sites
3gvdI

A

A

C

I

W

Y

I

L

E

Q

P

N

P

Q

I

V

N

S

I

V

A

A

Y

F

G

G

T

V

H

D

-

I

-

H

-

P

-

A

T

A

T

T

I

I

G

G

D

C

N

G

F

I

Y

M

A

L

N
x
D

F

H

N

A

L

T

T

G

L

I

V

V

D

I

D

G

A

E

D

T

I

A

I

V

G

E

N

N

N

D

V

V

M

S

F

I

A

L

P

Q

N

S

V

V

T

T

I

L

S
x
G

T
x
G

A

T

G

G

H

K

P

T

V

S

-

G

-

D

-
x
R

H

H

P

P

D

K

L

I

R

R

H

-

T

-

Q

-

F

-

S

-

Q

-

P

-

V

-

I

-

E

-

D

E

G

G

V

V

W

M

L

I

G

G

T

A

G

G

V

A

I

K

I

I

N

L

P

G

G

N

V

I

T

E

I

V

G

G

K

R

N

G

S

A

V

K

I

I

G

G

A

A

G

G

S

S

V

V

N

L

R

Q

S

S

I

V

P

P

A

A

N

H

V

T

A

A

G

G

V

V

P

P

C

A

R

R

I

I

L

V

binding cysteine: D160 (≠ N83), G186 (≠ S109), G187 (≠ T110), R195 (vs. gap)

Sites not aligning to the query:

binding cysteine: 95, 96

P71063 UDP-N-acetylbacillosamine N-acetyltransferase; EC 2.3.1.203 from Bacillus subtilis (strain 168) (see paper)
43% identity, 38% coverage: 115:188/196 of query aligns to 145:215/216 of P71063

query
sites
P71063

V

V

H
|
H

P

L

D

S

L

P

R

R

H

A

T

T

Q

-

F

L

S

S

Q

G

P

A

V

V

I

S

I

V

E

Q

D

E

G

G

V

A

W

H

L

V

G

G

T

T

G

G

V

A

I

S

I

V

N

I

P

P

G

Q

V

I

T

I

I

I

G

G

K

A

N

W

S

S

V

I

I

V

G

G

A

A

G

G

S

S

V

A

V

V

N

I

R

R

S

S

I

I

P

P

A

D

N

R

V

V

V

T

A

A

G

G

V

A

P

P

C

A

R

R

I

I

L

I

R

S

E

S

I

I

S

Q

D

T

E

S

D

N

K

K

H146 (= H116) mutation to L: Loss of activity. Affects the oligomeric state.

7l82AAA Putative acetyl transferase protein (see paper)
31% identity, 64% coverage: 55:180/196 of query aligns to 108:216/216 of 7l82AAA

query
sites
7l82AAA

S

S

V

I

G

D

K

A

A

G

C

A

W

A

I

L

E

S

P

P

P

F

I

V

N

T

I

I

A

A

Y

-

G

-

T

A

H

N

T

V

T

T

I

I

G

G

D

K

N

C

F

F

Y

H

A

A

N

N

F

L

N

Y

L

S

T

Y

L

V

V

-

D

-

D

E

A

H

D

D

I

C

I

I

G

G

N

D

N

Y

V

V

M

T

F

F

A

A

P

P

N

R

V

V

T

S

I

C

S

N

T

G

A

N

G

I

H

H

P

-

V

-

H

-

P

-

D

-

L

-

R

-

H

-

T

-

Q

-

F

-

S

-

Q

-

P

-

V

-

I

-

I

I

E

H

D

D

G

H

V

A

W
x
Y

L

I

G

G

T
|
T

G

G

V

A

I

V

I

I

N

K

P

Q

G

G

-

T

-

P

-

D

-

K

-

P

V

L

T

I

I

I

G

G

K

K

N

G

S

A

V

I

I

V

G
|
G

A
x
M

G

G

S

A

V

V

N

T

R

K

S

E

I

V

P

P

A

A

N

G

V

A

V

V

A

V

A
x
I

G

G

V

N

P

P

C

A

R

R

I

L

L

L

binding [(2~{R},3~{R},4~{S},5~{S},6~{R})-3-acetamido-5-[[(1~{S})-1-[2-[3-[[(2~{R})-4-[[[(2~{R},3~{S},4~{R},5~{R})-5-(6-aminopurin-9-yl)-4-oxidanyl-3-phosphonooxy-oxolan-2-yl]methoxy-oxidanyl-phosphoryl]oxy-oxidanyl-phosphoryl]oxy-3,3-dimethyl-2-oxidanyl-butanoyl]amino]propanoylamino]ethylsulfanyl]-1-oxidanyl-ethyl]amino]-6-methyl-4-oxidanyl-oxan-2-yl] [[(2~{R},3~{S},4~{R},5~{R})-5-[2,4-bis(oxidanylidene)pyrimidin-1-yl]-3,4-bis(oxidanyl)oxolan-2-yl]methoxy-oxidanyl-phosphoryl] hydrogen phosphate: Y168 (≠ W137), T171 (= T140), G193 (= G157), M194 (≠ A158), I209 (≠ A173)

Sites not aligning to the query:

binding [(2~{R},3~{R},4~{S},5~{S},6~{R})-3-acetamido-5-[[(1~{S})-1-[2-[3-[[(2~{R})-4-[[[(2~{R},3~{S},4~{R},5~{R})-5-(6-aminopurin-9-yl)-4-oxidanyl-3-phosphonooxy-oxolan-2-yl]methoxy-oxidanyl-phosphoryl]oxy-oxidanyl-phosphoryl]oxy-3,3-dimethyl-2-oxidanyl-butanoyl]amino]propanoylamino]ethylsulfanyl]-1-oxidanyl-ethyl]amino]-6-methyl-4-oxidanyl-oxan-2-yl] [[(2~{R},3~{S},4~{R},5~{R})-5-[2,4-bis(oxidanylidene)pyrimidin-1-yl]-3,4-bis(oxidanyl)oxolan-2-yl]methoxy-oxidanyl-phosphoryl] hydrogen phosphate: 10, 12, 39, 40, 73, 74

7l81AAA Putative acetyl transferase protein (see paper)
31% identity, 64% coverage: 55:180/196 of query aligns to 108:216/216 of 7l81AAA

query
sites
7l81AAA

S

S

V

I

G

D

K

A

A

G

C

A

W

A

I

L

E

S

P

P

P

F

I

V

N

T

I

I

A

A

Y

-

G

-

T

A

H

N

T

V

T

T

I

I

G

G

D

K

N

C

F

F

Y

H

A

A

N

N

F

L

N

Y

L

S

T

Y

L

V

V

-

D

-

D

E

A

H

D

D

I

C

I

I

G

G

N

D

N

Y

V

V

M

T

F

F

A
|
A

P

P

N

R

V

V

T

S

I

C

S

N

T

G

A

N

G

I

H

H

P

-

V

-

H

-

P

-

D

-

L

-

R

-

H

-

T

-

Q

-

F

-

S

-

Q

-

P

-

V

-

I

-

I

I

E

H

D

D

G

H

V

A

W
x
Y

L

I

G

G

T
|
T

G

G

V

A

I

V

I

I

N

K

P

Q

G

G

-

T

-

P

-

D

-

K

-

P

V

L

T

I

I

I

G

G

K

K

N

G

S

A

V

I

I

V

G
|
G

A
x
M

G

G

S

A

V

V

N

T

R

K

S

E

I

V

P

P

A

A

N

G

V

A

V

V

A

V

A
x
I

G

G

V

N

P

P

C

A

R

R

I

L

L

L

binding coenzyme a: A152 (= A103), Y168 (≠ W137), T171 (= T140), G193 (= G157), M194 (≠ A158), I209 (≠ A173)

Sites not aligning to the query:

binding UDP-2-acetamido-4-amino-2,4,6-trideoxy-alpha-D-glucopyranose: 10, 12, 39, 40, 73, 74

7l7zAAA Putative acetyl transferase protein (see paper)
31% identity, 64% coverage: 55:180/196 of query aligns to 108:216/216 of 7l7zAAA

query
sites
7l7zAAA

S

S

V

I

G

D

K

A

A

G

C

A

W

A

I

L

E

S

P

P

P

F

I

V

N

T

I

I

A

A

Y

-

G

-

T

A

H

N

T

V

T

T

I

I

G

G

D

K

N

C

F

F

Y

H

A

A

N

N

F

L

N

Y

L

S

T

Y

L

V

V

-

D

-

D

E

A

H

D

D

I

C

I

I

G

G

N

D

N

Y

V

V

M

T

F

F

A

A

P

P

N

R

V

V

T

S

I

C

S

N

T

G

A

N

G

I

H

H

P

-

V

-

H

-

P

-

D

-

L

-

R

-

H

-

T

-

Q

-

F

-

S

-

Q

-

P

-

V

-

I

-

I

I

E

H

D

D

G

H

V

A

W
x
Y

L

I

G

G

T
|
T

G

G

V

A

I

V

I

I

N

K

P

Q

G

G

-

T

-

P

-

D

-

K

-

P

V

L

T

I

I

I

G

G

K

K

N

G

S

A

V

I

I

V

G
|
G

A
x
M

G

G

S

A

V

V

N

T

R

K

S

E

I

V

P

P

A

A

N

G

V

A

V

V

A

V

A
x
I

G

G

V

N

P

P

C

A

R

R

I

L

L

L

binding coenzyme a: Y168 (≠ W137), T171 (= T140), G193 (= G157), M194 (≠ A158), I209 (≠ A173)

Sites not aligning to the query:

binding UDP-di-N-acetyl-alpha-bacillosamine: 10, 12, 39, 40, 73, 74

7l7yAAA Putative acetyl transferase protein (see paper)
31% identity, 64% coverage: 55:180/196 of query aligns to 108:216/217 of 7l7yAAA

query
sites
7l7yAAA

S

S

V

I

G

D

K

A

A

G

C

A

W

A

I

L

E

S

P

P

P

F

I

V

N

T

I

I

A

A

Y

-

G

-

T

A

H

N

T

V

T

T

I

I

G

G

D

K

N

C

F

F

Y

H

A

A

N

N

F

L

N

Y

L

S

T

Y

L

V

V

-

D

-

D

E

A

H

D

D

I

C

I

I

G

G

N

D

N

Y

V

V

M

T

F

F

A

A

P

P

N

R

V

V

T

S

I

C

S

N

T

G

A

N

G

I

H

H

P

-

V

-

H

-

P

-

D

-

L

-

R

-

H

-

T

-

Q

-

F

-

S

-

Q

-

P

-

V

-

I

-

I

I

E

H

D

D

G

H

V

A

W
x
Y

L

I

G
|
G

T
|
T

G

G

V

A

I

V

I

I

N

K

P

Q

G

G

-

T

-

P

-

D

-

K

-

P

V

L

T

I

I

I

G

G

K

K

N

G

S

A

V

I

I

V

G
|
G

A
x
M

G

G

S

A

V

V

N

T

R

K

S

E

I

V

P

P

A

A

N

G

V

A

V

V

A

V

A
x
I

G

G

V

N

P

P

C

A

R

R

I

L

L

L

binding acetyl coenzyme *a: Y168 (≠ W137), G170 (= G139), T171 (= T140), G193 (= G157), M194 (≠ A158), I209 (≠ A173)

Sites not aligning to the query:

binding uridine-5'-diphosphate: 10, 12, 39, 40, 73, 74

Q93S40 Polysialic acid O-acetyltransferase; Polysialyltransferase; PST; EC 2.3.1.- from Neisseria meningitidis (see paper)
31% identity, 65% coverage: 55:182/196 of query aligns to 67:191/215 of Q93S40

query
sites
Q93S40

S

N

V

M

G

G

K

L

A

V

C

C

W

S

I

L

E

H

P

S

P

D

I

C

N

S

I

L

A

Q

Y

I

G

Q

T

A

H

K

T

T

I

M

G

G

D

-

N

-

F

-

Y

-

A

-

N

N

F

G

N

E

L

I

T

T

L

I

V

A

D

E

D

K

A

G

D

K

I

I

I

S

I

I

G

G

N

K

N

D

V

C

M

M

F

L

A

A

P

H

N

G

V

Y

T

E

I

I

-

R

S

N

T

T

A
x
D

G
x
M

H
|
H

P

P

V

I

H

Y

P

S

D

L

L

E

R

N

H

G

T

E

Q

R

Q

I

Q

N

F

H

S

G

Q

K

P

D

V

V

I

I

E

G

D

N

G

H

V

V

W
|
W

L

L

G

G

T
x
R

G

N

V

V

I

T

I

I

N

L

P
x
K

G

G

V

V

T

C

I

I

G

P

K

N

N

N

S

V

V

V

I

V

G

G

A
x
S

G

H

S

T

V

V

V

L

N
x
Y

R
x
K

S

S

I

F

P

K

A

E

-

P

N

N

V

C

V

V

A

I

A

A

G

G

V

S

P

P

C

A

R

K

I

I

L

V

R
x
K

E

E

DMH 119:121 (≠ AGH 111:113) binding
H121 (= H113) mutation to A: Reduces activity 50-fold.
W145 (= W137) mutation to A: Reduces activity 56-fold.
R148 (≠ T140) binding
K154 (≠ P146) binding
S166 (≠ A158) binding
Y171 (≠ N163) mutation to A: Reduces activity 48-fold.
YK 171:172 (≠ NR 163:164) binding
K190 (≠ R181) binding

2wlgA Crystallographic analysis of the polysialic acid o- acetyltransferase oatwy (see paper)
31% identity, 65% coverage: 56:182/196 of query aligns to 64:187/211 of 2wlgA

query
sites
2wlgA

V

M

G

G

K

L

A

V

C

C

W

S

I

L

E

H

P

S

P

D

I

C

N

S

I

L

A

Q

Y

I

G

Q

T

A

H

K

T

T

I

M

G

G

D

-

N

-

F

-

Y

-

A

-

N

N

F

G

N

E

L

I

T

T

L

I

V

A

D

E

D

K

A

G

D

K

I

I

I

S

I

I

G

G

N

K

N

D

V

C

M

M

F

L

A

A

P

H

N

G

V

Y

T

E

I

I

-
x
R

S

N

T

T

A
x
D

G

M

H
|
H

P

P

V
x
I

H

Y

P

S

D

L

L

E

R

N

H

G

T

E

Q

R

Q

I

Q

N

F

H

S

G

Q

K

P

D

V

V

I

I

E

G

D

N

G

H

V

V

W

W

L

L

G

G

T

R

G

N

V

V

I

T

I

I

N

L

P
x
K

G

G

V

V

T

C

I

I

G

P

K

N

N

N

S

V

V

V

I

V

G

G

A

S

G

H

S

T

V

V

V

L

N
x
Y

R

K

S

S

I

F

P

K

A

E

-

P

N

N

V

C

V

V

A

I

A

A

G

G

V

S

P

P

C

A

R

K

I

I

L

V

R

K

E

E

binding [(2r,3s,4r,5r)-5-(6-amino-9h-purin-9-yl)-4-hydroxy-3-(phosphonooxy)tetrahydrofuran-2-yl]methyl(3r)-3-hydroxy-2,2-dimethyl-4-oxo-4-{[3-oxo-3-({2-[(2-oxopropyl)thio]ethyl}amino)propyl]amino}butyl dihydrogendiphosphate: R112 (vs. gap), D115 (≠ A111), H117 (= H113), I119 (≠ V115), K150 (≠ P146), Y167 (≠ N163)

2wleC Crystallographic analysis of the polysialic acid o- acetyltransferase oatwy (see paper)
31% identity, 65% coverage: 56:182/196 of query aligns to 64:187/211 of 2wleC

query
sites
2wleC

V

M

G

G

K

L

A

V

C

C

W

S

I

L

E

H

P

S

P

D

I

C

N

S

I

L

A

Q

Y

I

G

Q

T

A

H

K

T

T

I

M

G

G

D

-

N

-

F

-

Y

-

A

-

N

N

F

G

N

E

L

I

T

T

L

I

V

A

D

E

D

K

A

G

D

K

I

I

I

S

I

I

G

G

N

K

N

D

V

C

M

M

F

L

A
|
A

P

H

N

G

V

Y

T

E

I

I

-
x
R

S

N

T

T

A
x
D

G

M

H

H

P

P

V
x
I

H

Y

P

S

D

L

L

E

R

N

H

G

T

E

Q

R

Q

I

Q

N

F

H

S

G

Q

K

P

D

V

V

I

I

E

G

D

N

G

H

V

V

W
|
W

L

L

G

G

T
x
R

G

N

V

V

I

T

I

I

N

L

P
x
K

G

G

V

V

T

C

I

I

G

P

K

N

N

N

S

V

V

V

I

V

G
|
G

A
x
S

G

H

S

T

V

V

V

L

N
x
Y

R

K

S

S

I

F

P

K

A

E

-

P

N

N

V

C

V

V

A

I

A

A

G
|
G

V

S

P

P

C

A

R

K

I

I

L
x
V

R
x
K

E

E

binding coenzyme a: A106 (= A103), R112 (vs. gap), D115 (≠ A111), I119 (≠ V115), W141 (= W137), R144 (≠ T140), K150 (≠ P146), G161 (= G157), S162 (≠ A158), Y167 (≠ N163), G179 (= G174), V185 (≠ L180), K186 (≠ R181)

Query Sequence

>BWI76_RS00615 FitnessBrowser__Koxy:BWI76_RS00615
MDMKARMNSGELYLDSGYGLPEQRLMGKARAYEYNHSHPFNQELRDDIIRRMFASVGKAC
WIEPPINIAYGTHTTIGDNFYANFNLTLVDDADIIIGNNVMFAPNVTISTAGHPVHPDLR
HTQQQFSQPVIIEDGVWLGTGVIINPGVTIGKNSVIGAGSVVNRSIPANVVAAGVPCRIL
REISDEDKNKYQLFAG

Or try a new SitesBLAST search

SitesBLAST's Database

SitesBLAST's database includes (1) SwissProt entries with experimentally-supported functional features; and (2) protein structures with bound ligands, from the BioLip database.

by Morgan Price, Arkin group
Lawrence Berkeley National Laboratory