SitesBLAST
Comparing BWI76_RS00850 FitnessBrowser__Koxy:BWI76_RS00850 to proteins with known functional sites using BLASTp with E ≤ 0.001.
Or try Sites on a Tree, PaperBLAST, Conserved Domains, or compare to all protein structures
Found 20 (the maximum) hits to proteins with known functional sites (download)
2x2wA Acetylglutamate kinase from escherichia coli bound to n-acetyl-l- glutamyl-5-phosphate (see paper)
94% identity, 100% coverage: 1:257/257 of query aligns to 2:258/258 of 2x2wA
- active site: K8 (= K7), G11 (= G10), G45 (= G44), D162 (= D161), K217 (= K216)
- binding sulfate ion: G10 (= G9), G11 (= G10), V12 (= V11), S180 (= S179), K217 (= K216)
- binding n-acetyl-l-glutamyl 5-phosphate: K8 (= K7), G10 (= G9), G11 (= G10), G43 (= G42), G44 (= G43), G45 (= G44), R66 (= R65), N158 (= N157), V159 (= V158), A161 (= A160)
1oh9A Acetylglutamate kinase from escherichia coli complexed with mgadp, n- acetyl-l-glutamate and the transition-state mimic alf4- (see paper)
94% identity, 100% coverage: 1:257/257 of query aligns to 2:258/258 of 1oh9A
- active site: K8 (= K7), G11 (= G10), G45 (= G44), D162 (= D161), K217 (= K216)
- binding adenosine-5'-diphosphate: K8 (= K7), G10 (= G9), G11 (= G10), D162 (= D161), S180 (= S179), V182 (= V181), I185 (= I184), L186 (= L185), I209 (= I208), I210 (= I209), T211 (= T210), D212 (= D211), G213 (= G212), M214 (= M213), K217 (= K216)
- binding tetrafluoroaluminate ion: K8 (= K7), G10 (= G9), G11 (= G10), G43 (= G42), G44 (= G43), G45 (= G44)
- binding n-acetyl-l-glutamate: G43 (= G42), G44 (= G43), G45 (= G44), L65 (= L64), R66 (= R65), L80 (= L79), V122 (= V121), S147 (= S146), N158 (= N157), V159 (= V158), N160 (= N159), A161 (= A160)
1gs5A N-acetyl-l-glutamate kinase from escherichia coli complexed with its substrate n-acetylglutamate and its substrate analog amppnp (see paper)
94% identity, 100% coverage: 1:257/257 of query aligns to 2:258/258 of 1gs5A
- active site: K8 (= K7), G11 (= G10), G45 (= G44), D162 (= D161), K217 (= K216)
- binding phosphoaminophosphonic acid-adenylate ester: K8 (= K7), G10 (= G9), G11 (= G10), G43 (= G42), G44 (= G43), G45 (= G44), D162 (= D161), S180 (= S179), V182 (= V181), I185 (= I184), L186 (= L185), I209 (= I208), I210 (= I209), T211 (= T210), M214 (= M213), K217 (= K216)
- binding n-acetyl-l-glutamate: G43 (= G42), G44 (= G43), G45 (= G44), L65 (= L64), R66 (= R65), L80 (= L79), V122 (= V121), S147 (= S146), N158 (= N157), V159 (= V158), N160 (= N159), A161 (= A160)
P0A6C8 Acetylglutamate kinase; N-acetyl-L-glutamate 5-phosphotransferase; NAG kinase; NAGK; EC 2.7.2.8 from Escherichia coli (strain K12) (see 4 papers)
94% identity, 100% coverage: 1:257/257 of query aligns to 2:258/258 of P0A6C8
- K8 (= K7) mutation to R: Reduces activity 50-fold. Increases KM for N-acetyl-L-glutamate and ATP about 10-fold.
- GG 44:45 (= GG 43:44) binding
- R66 (= R65) binding ; mutation to K: Increases KM for N-acetyl-L-glutamate over 1000-fold. Increases KM for ATP nearly 20-fold.
- N158 (= N157) binding ; mutation to Q: Increases KM for N-acetyl-L-glutamate over 1000-fold. Increases KM for ATP over 20-fold.
- D162 (= D161) mutation to E: Reduces activity over 99%. No effect on KM for N-acetyl-L-glutamate and ATP.
- DVSGIL 181:186 (= DVSGIL 180:185) binding
- IIT 209:211 (= IIT 208:210) binding
Sites not aligning to the query:
- 1 modified: Initiator methionine, Removed
3t7bB Crystal structure of n-acetyl-l-glutamate kinase from yersinia pestis
85% identity, 99% coverage: 1:255/257 of query aligns to 2:256/258 of 3t7bB
- active site: K8 (= K7), G11 (= G10), G45 (= G44), D162 (= D161), K217 (= K216)
- binding glutamic acid: G43 (= G42), G44 (= G43), L65 (= L64), R66 (= R65), N158 (= N157), V159 (= V158), N160 (= N159), A161 (= A160)
2btyA Acetylglutamate kinase from thermotoga maritima complexed with its inhibitor arginine (see paper)
35% identity, 86% coverage: 4:225/257 of query aligns to 24:246/282 of 2btyA
- active site: K27 (= K7), G30 (= G10), G63 (= G44), D182 (= D161), K237 (= K216)
- binding arginine: K196 (vs. gap), S214 (≠ A193)
- binding n-acetyl-l-glutamate: K27 (= K7), G29 (= G9), G30 (= G10), G61 (= G42), G62 (= G43), G63 (= G44), N180 (= N159), D182 (= D161)
Sites not aligning to the query:
- binding arginine: 15, 19, 253, 266, 267, 269, 270, 271, 272, 274, 275, 276
Q9X2A4 Acetylglutamate kinase; N-acetyl-L-glutamate 5-phosphotransferase; NAG kinase; NAGK; EC 2.7.2.8 from Thermotoga maritima (strain ATCC 43589 / DSM 3109 / JCM 10099 / NBRC 100826 / MSB8) (see paper)
35% identity, 86% coverage: 4:225/257 of query aligns to 24:246/282 of Q9X2A4
- N178 (= N157) binding
- K196 (vs. gap) binding
- S214 (≠ A193) binding
Sites not aligning to the query:
2bufA Arginine feed-back inhibitable acetylglutamate kinase (see paper)
34% identity, 86% coverage: 4:225/257 of query aligns to 29:255/292 of 2bufA
- active site: K32 (= K7), G35 (= G10), G68 (= G44), D190 (= D161), K246 (= K216)
- binding n-acetyl-l-glutamate: G66 (= G42), G67 (= G43), I71 (≠ V47), M88 (≠ L64), R89 (= R65), N186 (= N157), A189 (= A160)
Q9HTN2 Acetylglutamate kinase; N-acetyl-L-glutamate 5-phosphotransferase; NAG kinase; NAGK; EC 2.7.2.8 from Pseudomonas aeruginosa (strain ATCC 15692 / DSM 22644 / CIP 104116 / JCM 14847 / LMG 12228 / 1C / PRS 101 / PAO1) (see 2 papers)
32% identity, 86% coverage: 4:225/257 of query aligns to 30:264/301 of Q9HTN2
- R90 (= R65) binding
- N195 (= N157) binding
Sites not aligning to the query:
- 1 modified: Initiator methionine, Removed
2bufC Arginine feed-back inhibitable acetylglutamate kinase (see paper)
32% identity, 86% coverage: 4:225/257 of query aligns to 29:263/298 of 2bufC
- active site: K32 (= K7), G35 (= G10), G68 (= G44), D198 (= D161), K254 (= K216)
- binding adenosine-5'-diphosphate: N36 (≠ V11), T217 (≠ S179), N218 (≠ D180), I219 (≠ V181), L222 (≠ I184), M223 (≠ L185), T246 (≠ I208), I247 (= I209), Y248 (≠ T210), G250 (= G212), M251 (= M213), K254 (= K216)
Q9SCL7 Acetylglutamate kinase, chloroplastic; N-acetyl-L-glutamate 5-phosphotransferase; NAG kinase; AtNAGK; EC 2.7.2.8 from Arabidopsis thaliana (Mouse-ear cress) (see 3 papers)
35% identity, 90% coverage: 4:235/257 of query aligns to 88:321/347 of Q9SCL7
- GA 94:95 (≠ GV 10:11) binding
- G126 (= G43) binding
- R148 (= R65) binding
- NINA 242:245 (≠ NVNA 157:160) binding
- K260 (≠ D174) binding
- TD 265:266 (≠ SD 179:180) binding
- L271 (= L185) binding
- K282 (≠ A193) binding
- 297:305 (vs. 208:216, 44% identical) binding
Sites not aligning to the query:
- 1:50 modified: transit peptide, Chloroplast
- 334:337 binding
- 342 binding
4usjB N-acetylglutamate kinase from arabidopsis thaliana in complex with pii from chlamydomonas reinhardtii (see paper)
35% identity, 90% coverage: 4:235/257 of query aligns to 22:255/281 of 4usjB
- active site: K25 (= K7), G28 (= G10), G61 (= G44), D180 (= D161), K239 (= K216)
- binding arginine: K194 (≠ D174), K216 (≠ A193)
- binding n-acetyl-l-glutamyl 5-phosphate: K25 (= K7), G27 (= G9), G28 (= G10), G59 (= G42), G60 (= G43), G61 (= G44), I64 (≠ V47), L81 (= L64), R82 (= R65), V140 (= V121), N176 (= N157), I177 (≠ V158), N178 (= N159), A179 (= A160)
Sites not aligning to the query:
2rd5B Structural basis for the regulation of n-acetylglutamate kinase by pii in arabidopsis thaliana (see paper)
35% identity, 90% coverage: 4:235/257 of query aligns to 24:257/283 of 2rd5B
- active site: K27 (= K7), G30 (= G10), G63 (= G44), D182 (= D161), K241 (= K216)
- binding adenosine-5'-diphosphate: K27 (= K7), G29 (= G9), G30 (= G10), A31 (≠ V11), T201 (≠ S179), D202 (= D180), V203 (= V181), G205 (= G183), I206 (= I184), L207 (= L185), K210 (= K188), K233 (≠ I208), V234 (≠ I209), A235 (≠ T210), G237 (= G212), M238 (= M213), K241 (= K216)
- binding arginine: K196 (≠ D174), K218 (≠ A193)
- binding n-acetyl-l-glutamate: G61 (= G42), G62 (= G43), G63 (= G44), I66 (≠ V47), L83 (= L64), R84 (= R65), L98 (= L79), N178 (= N157), I179 (≠ V158), N180 (= N159), A181 (= A160)
Sites not aligning to the query:
2v5hB Controlling the storage of nitrogen as arginine: the complex of pii and acetylglutamate kinase from synechococcus elongatus pcc 7942 (see paper)
33% identity, 98% coverage: 4:256/257 of query aligns to 25:282/289 of 2v5hB
- active site: K28 (= K7), G31 (= G10), G64 (= G44), D182 (= D161), K241 (= K216)
- binding n-acetyl-l-glutamate: G62 (= G42), G63 (= G43), G64 (= G44), I67 (≠ V47), L84 (= L64), R85 (= R65), N178 (= N157), I179 (≠ V158), N180 (= N159), A181 (= A160)
2bufK Arginine feed-back inhibitable acetylglutamate kinase (see paper)
33% identity, 86% coverage: 4:225/257 of query aligns to 29:240/273 of 2bufK
3l86A The crystal structure of smu.665 from streptococcus mutans ua159
33% identity, 84% coverage: 4:220/257 of query aligns to 5:219/245 of 3l86A
- active site: K8 (= K7), G11 (= G10), G42 (= G44), D160 (= D161), K215 (= K216)
- binding adenosine-5'-diphosphate: G10 (= G9), T179 (≠ S179), N180 (≠ D180), V181 (= V181), G183 (= G183), V184 (≠ I184), L185 (= L185), V207 (≠ I208), I208 (= I209), T209 (= T210), G211 (= G212), M212 (= M213), K215 (= K216)
- binding n-acetyl-l-glutamate: G40 (= G42), G41 (= G43), G42 (= G44), I45 (≠ V47), L62 (= L64), R63 (= R65), S145 (= S146), N156 (= N157), I157 (≠ V158), N158 (= N159), A159 (= A160)
7nloA Crystal structure of mycobacterium tuberculosis argb in complex with l-arginine
30% identity, 86% coverage: 4:223/257 of query aligns to 26:253/289 of 7nloA
Sites not aligning to the query:
- binding arginine: 17, 262, 275, 276, 278, 279, 280, 281, 283, 284, 285
7nlyA Crystal structure of mycobacterium tuberculosis argb in complex with 2-chlorobenzimidazole.
30% identity, 86% coverage: 4:223/257 of query aligns to 30:257/293 of 7nlyA