SitesBLAST
Comparing BWI76_RS01010 FitnessBrowser__Koxy:BWI76_RS01010 to proteins with known functional sites using BLASTp with E ≤ 0.001.
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Found 20 (the maximum) hits to proteins with known functional sites (download)
4mchA Crystal structure of uridine phosphorylase from vibrio fischeri es114 complexed with 6-hydroxy-1-naphthoic acid, nysgrc target 029520.
46% identity, 87% coverage: 1:217/249 of query aligns to 6:221/243 of 4mchA
- active site: H11 (= H6), V23 (≠ A19), G27 (= G23), R31 (= R27), R49 (= R45), N55 (≠ R51), G56 (= G52), E81 (= E77), R92 (= R88), S95 (= S91), D169 (≠ A165), V208 (= V204), V209 (= V205), Y211 (≠ H207), Y218 (≠ S214)
- binding 6-hydroxynaphthalene-1-carboxylic acid: R34 (= R30), L38 (≠ F34)
Sites not aligning to the query:
1rxsB E. Coli uridine phosphorylase: 2'-deoxyuridine phosphate complex (see paper)
36% identity, 94% coverage: 6:239/249 of query aligns to 5:241/246 of 1rxsB
- active site: H5 (= H6), R27 (= R27), R45 (= R45), E77 (= E77), R88 (= R88), R165 (≠ E166), I217 (≠ V204), V218 (= V205), R220 (≠ Y218)
- binding 2'-deoxyuridine: T91 (≠ S91), T92 (≠ C92), G93 (= G93), F159 (= F159), Q163 (≠ R163), M194 (= M181), E195 (= E182)
- binding potassium ion: E46 (= E46), G65 (= G65), I66 (= I66), S70 (≠ F70)
- binding meta vanadate: R175 (vs. gap), H176 (vs. gap)
1rxcA E. Coli uridine phosphorylase: 5-fluorouracil ribose-1-phosphate complex (see paper)
36% identity, 94% coverage: 6:239/249 of query aligns to 5:241/246 of 1rxcA
- active site: H5 (= H6), G23 (= G23), R27 (= R27), R45 (= R45), E77 (= E77), R88 (= R88), T91 (≠ S91), R165 (≠ E166), I217 (≠ V204), V218 (= V205), R220 (≠ Y218)
- binding potassium ion: E46 (= E46), G65 (= G65), I66 (= I66), S70 (≠ F70)
- binding 1-O-phosphono-alpha-D-ribofuranose: H5 (= H6), R45 (= R45)
4yjkA Crystal structure of c212s mutant of shewanella oneidensis mr-1 uridine phosphorylase (see paper)
40% identity, 80% coverage: 6:205/249 of query aligns to 5:218/245 of 4yjkA
- active site: H5 (= H6), G23 (= G23), R27 (= R27), R45 (= R45), E77 (= E77), R88 (= R88), T91 (≠ S91), R165 (vs. gap), I217 (≠ V204), V218 (= V205)
- binding uracil: T91 (≠ S91), T92 (≠ C92), G93 (= G93), F159 (= F159), Q163 (vs. gap), R165 (vs. gap), E193 (≠ D180)
Sites not aligning to the query:
2hwuA Crystal structure of the uridine phosphorylase from salmonella typhimurium in complex with uridine and phosphate ion at 2.91a resolution
39% identity, 80% coverage: 6:205/249 of query aligns to 8:221/253 of 2hwuA
- active site: R30 (= R27), R48 (= R45), R91 (= R88), R168 (≠ E166), I220 (≠ V204), V221 (= V205)
- binding uridine: T94 (≠ S91), T95 (≠ C92), G96 (= G93), F162 (= F159), Q166 (≠ R163), E196 (≠ D180), M197 (= M181), E198 (= E182)
2hn9A Crystal structure of the uridine phosphorylase from salmonella typhimurium in complex with thymine and phosphate ion at 2.12a resolution
39% identity, 80% coverage: 6:205/249 of query aligns to 8:221/253 of 2hn9A
1y1qC Crystal structure of the uridine phosphorylase from salmonella typhimurium in complex with uridine-5p-monophosphate and sulfate ion at 2.35a resolution
39% identity, 80% coverage: 6:205/249 of query aligns to 5:218/240 of 1y1qC
- active site: R27 (= R27), R45 (= R45), R88 (= R88), R165 (≠ E166), I217 (≠ V204), V218 (= V205)
- binding uridine-5'-monophosphate: R88 (= R88), T91 (≠ S91), T92 (≠ C92), G93 (= G93), F159 (= F159), Q163 (≠ R163), Y192 (≠ A179), E193 (≠ D180), M194 (= M181)
1zl2B Crystal structure of the uridine phosphorylase from salmonella typhimurium in complex with 2,2'-anhydrouridine and phosphate ion at 1.85a resolution
39% identity, 80% coverage: 6:205/249 of query aligns to 5:218/242 of 1zl2B
- active site: R27 (= R27), R45 (= R45), R88 (= R88), R165 (≠ E166), I217 (≠ V204), V218 (= V205)
- binding 2,2'-Anhydro-(1-beta-D-arabinofuranosyl)uracil: T91 (≠ S91), T92 (≠ C92), F159 (= F159), Q163 (≠ R163), R165 (≠ E166), E193 (≠ D180), M194 (= M181), E195 (= E182), I217 (≠ V204)
1y1rB Crystal structure of the uridine phosphorylase from salmonella typhimurium in complex with inhibitor and phosphate ion at 2.11a resolution
39% identity, 80% coverage: 6:205/249 of query aligns to 5:218/242 of 1y1rB
- active site: H5 (= H6), G23 (= G23), R27 (= R27), R45 (= R45), E77 (= E77), R88 (= R88), T91 (≠ S91), R165 (≠ E166), I217 (≠ V204), V218 (= V205)
- binding 2,2'-Anhydro-(1-beta-D-ribofuranosyl)uracil: I66 (= I66), T91 (≠ S91), T92 (≠ C92), G93 (= G93), F159 (= F159), Q163 (≠ R163), R165 (≠ E166), E193 (≠ D180), M194 (= M181), E195 (= E182), I217 (≠ V204)
Sites not aligning to the query:
1u1eA Structure of e. Coli uridine phosphorylase complexed to 5(phenylseleno)acyclouridine (psau) (see paper)
39% identity, 80% coverage: 6:205/249 of query aligns to 8:221/253 of 1u1eA
- active site: H8 (= H6), G26 (= G23), R30 (= R27), R48 (= R45), E80 (= E77), R91 (= R88), T94 (≠ S91), R168 (≠ E166), I220 (≠ V204), V221 (= V205)
- binding 1-((2-hydroxyethoxy)methyl)-5-(phenylselanyl)pyrimidine-2,4(1h,3h)-dione: H8 (= H6), T94 (≠ S91), T95 (≠ C92), G96 (= G93), F162 (= F159), Q166 (≠ R163), R168 (≠ E166), E196 (≠ D180), M197 (= M181), I220 (≠ V204)
- binding phosphate ion: G26 (= G23), R30 (= R27), R91 (= R88), G93 (= G90), T94 (≠ S91)
Sites not aligning to the query:
1u1dA Structure of e. Coli uridine phosphorylase complexed to 5- (phenylthio)acyclouridine (ptau) (see paper)
39% identity, 80% coverage: 6:205/249 of query aligns to 8:221/253 of 1u1dA
- active site: H8 (= H6), G26 (= G23), R30 (= R27), R48 (= R45), E80 (= E77), R91 (= R88), T94 (≠ S91), R168 (≠ E166), I220 (≠ V204), V221 (= V205)
- binding 1-((2-hydroxyethoxy)methyl)-5-(phenylthio)pyrimidine-2,4(1h,3h)-dione: H8 (= H6), T94 (≠ S91), T95 (≠ C92), G96 (= G93), F162 (= F159), Q166 (≠ R163), R168 (≠ E166), M197 (= M181), I220 (≠ V204)
- binding phosphate ion: G26 (= G23), R30 (= R27), R91 (= R88), G93 (= G90), T94 (≠ S91)
Sites not aligning to the query:
1u1cA Structure of e. Coli uridine phosphorylase complexed to 5- benzylacyclouridine (bau) (see paper)
39% identity, 80% coverage: 6:205/249 of query aligns to 8:221/253 of 1u1cA
- active site: H8 (= H6), G26 (= G23), R30 (= R27), R48 (= R45), E80 (= E77), R91 (= R88), T94 (≠ S91), R168 (≠ E166), I220 (≠ V204), V221 (= V205)
- binding 1-((2-hydroxyethoxy)methyl)-5-benzylpyrimidine-2,4(1h,3h)-dione: H8 (= H6), T94 (≠ S91), T95 (≠ C92), G96 (= G93), F162 (= F159), Q166 (≠ R163), R168 (≠ E166), E196 (≠ D180), I220 (≠ V204)
- binding phosphate ion: G26 (= G23), R30 (= R27), R91 (= R88), G93 (= G90), T94 (≠ S91), E198 (= E182)
Sites not aligning to the query:
P12758 Uridine phosphorylase; UPase; UrdPase; EC 2.4.2.3 from Escherichia coli (strain K12) (see 3 papers)
39% identity, 80% coverage: 6:205/249 of query aligns to 8:221/253 of P12758
Sites not aligning to the query:
- 1 modified: Initiator methionine, Removed
- 5 mutation D->A,E,N: No change in activity.
1u1gA Structure of e. Coli uridine phosphorylase complexed to 5-(m- (benzyloxy)benzyl)barbituric acid (bbba) (see paper)
39% identity, 80% coverage: 6:205/249 of query aligns to 5:218/245 of 1u1gA
- active site: H5 (= H6), G23 (= G23), R27 (= R27), R45 (= R45), E77 (= E77), R88 (= R88), T91 (≠ S91), R165 (≠ E166), I217 (≠ V204), V218 (= V205)
- binding 1-((2-hydroxyethoxy)methyl)-5-(3-(benzyloxy)benzyl)-6-hydroxypyrimidine-2,4(1h,3h)-dione: T91 (≠ S91), T92 (≠ C92), F159 (= F159), Q163 (≠ R163), R165 (≠ E166), M194 (= M181), I217 (≠ V204), V218 (= V205)
Sites not aligning to the query:
3kvvA Trapping of an oxocarbenium ion intermediate in up crystals (see paper)
39% identity, 80% coverage: 6:205/249 of query aligns to 5:218/243 of 3kvvA
- active site: H5 (= H6), G23 (= G23), R27 (= R27), R45 (= R45), E77 (= E77), R88 (= R88), T91 (≠ S91), R165 (≠ E166), I217 (≠ V204), V218 (= V205)
- binding 1,4-anhydro-D-erythro-pent-1-enitol: T91 (≠ S91), F159 (= F159), E193 (≠ D180), M194 (= M181), E195 (= E182)
- binding sulfate ion: G23 (= G23), R27 (= R27), R88 (= R88), G90 (= G90), T91 (≠ S91)
- binding 5-fluorouracil: T91 (≠ S91), T92 (≠ C92), G93 (= G93), F159 (= F159), Q163 (≠ R163), R165 (≠ E166), I217 (≠ V204), V218 (= V205)
Sites not aligning to the query:
1u1fA Structure of e. Coli uridine phosphorylase complexed to 5-(m- (benzyloxy)benzyl)acyclouridine (bbau) (see paper)
39% identity, 80% coverage: 6:205/249 of query aligns to 5:218/243 of 1u1fA
- active site: H5 (= H6), G23 (= G23), R27 (= R27), R45 (= R45), E77 (= E77), R88 (= R88), T91 (≠ S91), R165 (≠ E166), I217 (≠ V204), V218 (= V205)
- binding 1-((2-hydroxyethoxy)methyl)-5-(3-(benzyloxy)benzyl)pyrimidine-2,4(1h,3h)-dione: H5 (= H6), R45 (= R45), T91 (≠ S91), T92 (≠ C92), G93 (= G93), F159 (= F159), Q163 (≠ R163), R165 (≠ E166), E193 (≠ D180), M194 (= M181), I217 (≠ V204), V218 (= V205)
- binding phosphate ion: G23 (= G23), R27 (= R27), R88 (= R88), G90 (= G90), T91 (≠ S91)
Sites not aligning to the query:
1tgyA Structure of e. Coli uridine phosphorylase complexed with uracil and ribose 1-phosphate
39% identity, 80% coverage: 6:205/249 of query aligns to 5:218/250 of 1tgyA
- active site: H5 (= H6), G23 (= G23), R27 (= R27), R45 (= R45), E77 (= E77), R88 (= R88), T91 (≠ S91), R165 (≠ E166), I217 (≠ V204), V218 (= V205)
- binding 1-O-phosphono-alpha-D-ribofuranose: H5 (= H6), G23 (= G23), R27 (= R27), R45 (= R45), R88 (= R88), T91 (≠ S91), F159 (= F159), M194 (= M181), E195 (= E182)
- binding uracil: G93 (= G93), F159 (= F159), Q163 (≠ R163), R165 (≠ E166)
Sites not aligning to the query:
1rxcJ E. Coli uridine phosphorylase: 5-fluorouracil ribose-1-phosphate complex (see paper)
39% identity, 80% coverage: 6:205/249 of query aligns to 5:218/250 of 1rxcJ
- active site: H5 (= H6), G23 (= G23), R27 (= R27), R45 (= R45), E77 (= E77), R88 (= R88), T91 (≠ S91), R165 (≠ E166), I217 (≠ V204), V218 (= V205)
- binding 5-fluorouridine: I66 (= I66), T91 (≠ S91), T92 (≠ C92), G93 (= G93), F159 (= F159), Q163 (≠ R163), E193 (≠ D180), M194 (= M181), E195 (= E182), I217 (≠ V204)
- binding potassium ion: E46 (= E46), G65 (= G65), I66 (= I66), S70 (≠ F70)
- binding phosphate ion: G23 (= G23), R27 (= R27), R88 (= R88), G90 (= G90), T91 (≠ S91)
- binding 1-O-phosphono-alpha-D-ribofuranose: H5 (= H6), R45 (= R45)
Sites not aligning to the query:
1rxcB E. Coli uridine phosphorylase: 5-fluorouracil ribose-1-phosphate complex (see paper)
39% identity, 80% coverage: 6:205/249 of query aligns to 5:218/250 of 1rxcB
- active site: H5 (= H6), G23 (= G23), R27 (= R27), R45 (= R45), E77 (= E77), R88 (= R88), T91 (≠ S91), R165 (≠ E166), I217 (≠ V204), V218 (= V205)
- binding potassium ion: E46 (= E46), G65 (= G65), I66 (= I66), S70 (≠ F70)
- binding 1-O-phosphono-alpha-D-ribofuranose: G23 (= G23), R27 (= R27), R88 (= R88), T91 (≠ S91), E193 (≠ D180), M194 (= M181), E195 (= E182)
- binding 5-fluorouracil: T91 (≠ S91), T92 (≠ C92), G93 (= G93), F159 (= F159), Q163 (≠ R163), I217 (≠ V204), V218 (= V205)
Sites not aligning to the query:
1rxsa E. Coli uridine phosphorylase: 2'-deoxyuridine phosphate complex (see paper)
39% identity, 80% coverage: 6:205/249 of query aligns to 5:218/242 of 1rxsa
- active site: H5 (= H6), R27 (= R27), R45 (= R45), E77 (= E77), R88 (= R88), R165 (≠ E166), I217 (≠ V204), V218 (= V205)
- binding 2'-deoxyuridine: I66 (= I66), T91 (≠ S91), T92 (≠ C92), G93 (= G93), F159 (= F159), Q163 (≠ R163), R165 (≠ E166), M194 (= M181), E195 (= E182)
- binding potassium ion: E46 (= E46), G65 (= G65), I66 (= I66), S70 (≠ F70)
Sites not aligning to the query:
Query Sequence
>BWI76_RS01010 FitnessBrowser__Koxy:BWI76_RS01010
MSLQPHIQLSKEMTGARYALLPGDPQRVDRIANFLDNPQPLGQNREFRAARGWYQGVEIL
VLSTGIGGPFTAIAIEELRQIGVTTLIRIGSCGALQDNLALGDVIIAHAAVMDDGTSRTY
APAGYPACADPQLTWELMAQARQMNIPAACGLVRSHDSFYTDREAELDAQWSARGILGAD
MESAALMTIGALRGLRTASLLNVVVAHNGCLDSSINDYVQQEALCQQGEERQITLALRAI
YSASQQGGL
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SitesBLAST's Database
SitesBLAST's database includes
(1) SwissProt
entries with experimentally-supported functional features;
and (2) protein structures with bound ligands, from the
BioLip database.
by Morgan Price,
Arkin group
Lawrence Berkeley National Laboratory