SitesBLAST

SitesBLAST – Find functional sites

SitesBLAST

Comparing BWI76_RS01510 FitnessBrowser__Koxy:BWI76_RS01510 to proteins with known functional sites using BLASTp with E ≤ 0.001.

Or try Sites on a Tree, PaperBLAST, Conserved Domains, or compare to all protein structures

Found 1 hits to proteins with known functional sites (download)

P0ABN9 Anaerobic C4-dicarboxylate transporter DcuB from Escherichia coli (strain K12) (see paper)
94% identity, 97% coverage: 15:446/446 of query aligns to 15:446/446 of P0ABN9

query
sites
P0ABN9

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E79 (= E79) mutation to A: Loses transport activity but still requires fumarate for expression.
R83 (= R83) mutation to A: Loses transport activity but still requires fumarate for expression.
R127 (= R127) mutation to A: Is able to catalyze uptake, but has lost the capacity for antiport activity.
K353 (= K353) mutation to A: Constitutively expressed.
T394 (= T394) mutation to A: Shows partial expression without fumarate. Impaired for transport to some extent.; mutation to I: Constitutively expressed. Affects regulation of the DcuS-DcuR activity, but not the transport activity.; mutation T->N,S: Requires fumarate for expression. Shows wild-type transport activity.
D398 (= D398) mutation to A: Requires fumarate for expression. Shows wild-type transport activity.; mutation to E: Requires fumarate for expression. Impaired for transport to some extent.; mutation to N: Constitutively expressed. Affects regulation of the DcuS-DcuR activity, but not the transport activity.

Query Sequence

>BWI76_RS01510 FitnessBrowser__Koxy:BWI76_RS01510
MEFAIQLIIILICLFYGAKKGGIALGLLGGIGLVILVFVFHLKPGKPPVDVMLVIIAVVA
ASATLQASGGLDVMLQIAEKLLRRNPKYVSIVAPFVTCTLTILCGTGHVVYTILPIIYDV
AIKNNIRPERPMAASSIGAQMGIIASPVSVAVVSLVAMLGNFTFNGKHLEFLDLLAITIP
STLLGILAIGIFSWFRGKDLDKDPEFQAFIAVPENRHYVYGDTATLLDKKLPTSNWIAMW
IFLASIAVVALLGAFSELRPVVDGKALSMVLVIQMFMLLSGALIIIITKTNPASISKNEV
FRSGMIAIVAVYGIAWMAETMFGAHMTEIKGVLGEMVKEYPWAYAIVLLLVSKFVNSQAA
ALAAIVPVALAIGVDPAYIVASAPACYGYYILPTYPSDLAAIQFDRSGTTHIGRFVINHS
FILPGLIGVSVSCVFGWVFAAMYGFL

Or try a new SitesBLAST search

SitesBLAST's Database

SitesBLAST's database includes (1) SwissProt entries with experimentally-supported functional features; and (2) protein structures with bound ligands, from the BioLip database.

by Morgan Price, Arkin group
Lawrence Berkeley National Laboratory