SitesBLAST

SitesBLAST – Find functional sites

SitesBLAST

Comparing BWI76_RS01820 BWI76_RS01820 maltose transporter permease to proteins with known functional sites using BLASTp with E ≤ 0.001.

Or try Sites on a Tree, PaperBLAST, Conserved Domains, or compare to all protein structures

Found 2 hits to proteins with known functional sites (download)

P68183 Maltose/maltodextrin transport system permease protein MalG from Escherichia coli (strain K12) (see 2 papers)
93% identity, 100% coverage: 1:296/296 of query aligns to 1:296/296 of P68183

query
sites
P68183

M

M

A

A

M

M

V

V

Q

Q

P

P

K

K

S

S

Q

Q

K

K

L

A

R

R

L

L

F

F

T

I

T

T

H

H

L

L

L

L

L

L

L

L

V

L

F

F

I

I

A

A

A

A

I

I

M

M

F

F

P

P

L

L

L

L

M

M

V

V

I

V

A

A

I

I

S

S

L

L

R

R

E

Q

G

G

N

N

F

F

A

A

T

T

G

G

S

S

L

L

I

I

P

P

E

E

S

Q

I

I

S

S

W

W

E

D

H

H

W

W

R

K

L

L

A

A

L

L

G

G

F

F

S

S

V

V

E

E

H

Q

A

A

D

D

G

G

R

R

V

I

T

T

P

P

P

P

P

P

F

F

P

P

V

V

L

L

L

L

W

W

L

L

W

W

N

N

S

S

I

V

K

K

V

V

A

A

G

G

I

I

T

S

A

A

I

I

G

G

I

I

V

V

A

A

L

L

S

S

T

T

T

T

C

C

A

A

Y

Y

A

A

F

F

A

A

R

R

M

M

R

R

F

F

P

P

G

G

K

K

A

A

T

T

L

L

L

L

K

K

G

G

M

M

L

L

I

I

F

F

Q

Q

M

M

F

F

P

P

A

A

V

V

L

L

S

S

L

L

V

V

A

A

L

L

Y

Y

A

A

L

L

F

F

D

D

R

R

L

L

G

G

Q

E

Y

Y

V

I

P

P

F

F

V

I

G

G

L

L

N

N

T

T

H

H

G

G

G

G

V

V

I

I

F

F

A

A

Y

Y

M

L

G

G

G

G

I

I

A

A

L

L

H

H

V

V

W

W

T

T

I

I

K

K

G

G

Y

Y

F

F

E

E

T

T

I

I

D

D

G

S

S

S

L

L

E

E

E
|
E

A

A

A
|
A

A

A

L

L

D

D

G
|
G

A

A

T

T

P

P

W

W

Q

Q

A

A

F

F

R

R

L

L

V

V

L

L

L

L

P
|
P

L

L

S

S

V

V

P

P

I

I

L

L

A

A

V

V

V

V

F

F

I

I

L

L

S

S

F

F

I

I

A

A

A

A

I

I

T

T

E

E

V

V

P

P

V

V

A

A

S

S

L

L

L

L

L

L

R

R

D

D

V

V

N

N

S

S

Y

Y

T

T

L

L

A

A

V

V

G

G

M

M

Q

Q

Q

Q

Y

Y

L

L

N

N

P

P

Q

Q

N

N

Y

Y

L

L

W

W

G

G

D

D

F

F

A

A

A

A

A

A

A

A

V

V

L

M

S

S

A

A

I

L

P

P

I

I

T

T

V

I

V

V

F

F

L

L

L

L

A

A

Q

Q

R

R

W

W

L

L

V

V

N

N

G

G

L

L

T

T

A

A

G

G

G

G

V

V

K

K

G

G

E190 (= E190) mutation E->A,C,K,L: Reduction of transport rate.
A192 (= A192) mutation A->D,S,L: Loss of transport and MalK dissociation from the membrane.
G196 (= G196) mutation to A: No effect.; mutation to P: Loss of transport and MalK dissociation from the membrane.
P209 (= P209) mutation to A: No effect.

P94530 Arabinooligosaccharides transport system permease protein AraQ from Bacillus subtilis (strain 168) (see paper)
24% identity, 72% coverage: 84:296/296 of query aligns to 76:281/281 of P94530

query
sites
P94530

W

W

L

F

W

F

N

N

S

S

I

L

K

V

V

L

A

G

G

L

I

F

T

T

A

T

I

V

G

L

I

T

V

L

A

F

L

F

S

S

T

S

T

M

C

I

A

G

Y

Y

A

G

F

L

A

A

R

V

M

Y

R

D

F

F

P

K

G

G

K

R

A

N

T

I

L

I

L

F

K

V

G

L

M

V

L

L

I

I

F

I

Q

M

M

M

F

V

P

P

A

L

V

E

L

V

S

M

L

M

V

L

A

P

L

L

Y

F

A

K

L

L

F

-

D

-

R

T

L

V

G

G

Q

L

Y

H

V

L

P

-

F

-

V

-

G

-

L

I

N

D

T

S

H

Y

G

T

G

G

V

V

I

I

F

L

A

P

Y

F

M

I

G

V

G

S

I

-

A

P

L

V

H

A

V

V

W

F

T

F

I

F

K

R

G

Q

Y

Y

F

A

E

L

T

G

I

L

D

P

G

R

S

D

L

L

E

L

E

D

A

S

A

A

A

R

L

M

D
|
D

G

G

A

C

T

T

P

E

W

F

Q

G

A

I

F

F

R

F

L

R

V

I

L

M

L

A

P

P

L

L

S

M

V

K

P

P

I

A

L

F

A

G

V

A

V

M

F

I

I

I

L

L

S

Q

F

S

I

L

A

N

A

S

I

W

T

N

E

N

V

F

P

L

V

W

A

P

S

L

L

I

L

V

L

L

R

R

D

S

V

K

N

E

S

M

Y

F

T

T

L

L

A

P

V

I

G

G

M

L

Q

S

Q

S

Y

L

L

L

N

S

P

P

Q

Y

N

G

Y

N

L

N

W

Y

G

D

D

M

F

L

A

I

A

S

A

G

A

S

V

V

L

F

S

A

A

I

I

L

P

P

I

V

T

I

V

I

V

I

F

F

L

L

L

F

A

F

Q

Q

R

K

W

Y

L

F

V

I

N

S

G

G

L

L

T

T

A

V

G

G

G

G

V

V

K

K

G

G

D180 (= D195) mutation to A: Negative impact in the growth rate in the presence of arabinotriose.

Query Sequence

>BWI76_RS01820 BWI76_RS01820 maltose transporter permease
MAMVQPKSQKLRLFTTHLLLLVFIAAIMFPLLMVIAISLREGNFATGSLIPESISWEHWR
LALGFSVEHADGRVTPPPFPVLLWLWNSIKVAGITAIGIVALSTTCAYAFARMRFPGKAT
LLKGMLIFQMFPAVLSLVALYALFDRLGQYVPFVGLNTHGGVIFAYMGGIALHVWTIKGY
FETIDGSLEEAAALDGATPWQAFRLVLLPLSVPILAVVFILSFIAAITEVPVASLLLRDV
NSYTLAVGMQQYLNPQNYLWGDFAAAAVLSAIPITVVFLLAQRWLVNGLTAGGVKG

Or try a new SitesBLAST search

SitesBLAST's Database

SitesBLAST's database includes (1) SwissProt entries with experimentally-supported functional features; and (2) protein structures with bound ligands, from the BioLip database.

by Morgan Price, Arkin group
Lawrence Berkeley National Laboratory