SitesBLAST
Comparing BWI76_RS02085 FitnessBrowser__Koxy:BWI76_RS02085 to proteins with known functional sites using BLASTp with E ≤ 0.001.
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Found 8 hits to proteins with known functional sites (download)
P07117 Sodium/proline symporter; Proline carrier; Proline permease; Propionate transporter from Escherichia coli (strain K12) (see 4 papers)
25% identity, 81% coverage: 36:479/549 of query aligns to 8:448/502 of P07117
- R257 (= R284) mutation to C: Sodium-independent binding affinity for proline.
- C281 (≠ T312) mutation to S: Does not affect proline uptake activity. Confers resistance to N-ethylmaleimide. Na(+)-dependent proline binding activity is similar to wild-type carrier.
- C344 (≠ G374) mutation to S: Small decrease in proline uptake activity. Confers resistance to N-ethylmaleimide. Exhibits low Na(+)-dependent proline binding.
- C349 (≠ G379) mutation to S: Does not affect proline uptake activity. Sensitive to N-ethylmaleimide. Na(+)-dependent proline binding activity is similar to wild-type carrier.
- R376 (≠ K406) mutation R->E,Q: No change in activity.; mutation to K: Loss of activity.
5nv9A Substrate-bound outward-open state of a na+-coupled sialic acid symporter reveals a novel na+-site (see paper)
19% identity, 69% coverage: 42:421/549 of query aligns to 13:389/480 of 5nv9A
- binding sodium ion: A52 (≠ G81), T53 (≠ D82), L55 (≠ M84), S56 (= S85), V174 (≠ T204), D178 (≠ Q208), A335 (≠ T367), S338 (≠ A370), S338 (≠ A370), S339 (≠ V371), S341 (≠ A373), S342 (≠ G374)
- binding N-acetyl-beta-neuraminic acid: T54 (≠ F83), S56 (= S85), I58 (≠ A87), T59 (≠ S88), G77 (≠ Y106), Q78 (≠ S107), R131 (≠ Q165), F239 (≠ L271)
Q92911 Sodium/iodide cotransporter; Na(+)/I(-) cotransporter; Natrium iodide transporter; Sodium-iodide symporter; Na(+)/I(-) symporter; Solute carrier family 5 member 5 from Homo sapiens (Human) (see 3 papers)
26% identity, 29% coverage: 58:218/549 of query aligns to 42:201/643 of Q92911
- A102 (≠ L118) natural variant: A -> P
Sites not aligning to the query:
- 226 mutation H->A,D,E,K: Significant loss of iodide transport activity but no effect on its localization to the cell membrane.
- 237 D→A: Loss of localization to the cell membrane, significant loss of iodide transport activity but no effect on homodimerization.
- 242 Required for homodimerization; Y→A: Loss of localization to the cell membrane, significant loss of iodide transport activity but no effect on homodimerization. Reduced homodimerization; when associated with A-471. Loss of iodide transport activity; when associated with F-535.
- 243 Required for homodimerization; T→A: Loss of localization to the cell membrane, significant loss of iodide transport activity but no effect on homodimerization. Reduced homodimerization; when associated with A-471.
- 471 Required for homodimerization; Q→A: No effect on localization to the cell membrane, iodide transport activity and homodimerization. Significant loss of homodimerization; when associated with A-242 or A243.
- 525 A→F: Loss of localization to the cell membrane, significant loss of iodide transport activity but no effect on homodimerization. Loss of iodide transport activity; when associated with A-242.
- 536 T → Q: requires 2 nucleotide substitutions
- 556 S → Q: requires 2 nucleotide substitutions
7sl8A Cryoem structure of sglt1 at 3.4 a resolution (see paper)
21% identity, 53% coverage: 173:461/549 of query aligns to 137:453/582 of 7sl8A
Sites not aligning to the query:
7slaA Cryoem structure of sglt1 at 3.15 angstrom resolution (see paper)
21% identity, 53% coverage: 173:461/549 of query aligns to 138:454/585 of 7slaA
Sites not aligning to the query:
Q9GZV3 High affinity choline transporter 1; hCHT1; Hemicholinium-3-sensitive choline transporter; CHT; Solute carrier family 5 member 7 from Homo sapiens (Human) (see 5 papers)
21% identity, 79% coverage: 34:464/549 of query aligns to 6:438/580 of Q9GZV3
- D48 (≠ I70) to G: in CMS20; decreased choline transmembrane transporter activity; no effect on localization at plasma membrane; dbSNP:rs886039768
- G65 (≠ A86) to E: in CMS20; loss of choline transmembrane transporter activity; no effect on localization at plasma membrane; dbSNP:rs886039765
- I89 (≠ V112) to V: 40% reduction in choline transmembrane transporter activity; found in 0.06 of Ashkenazi Jews; dbSNP:rs1013940; mutation to A: Decreased choline transmembrane transporter activity, only 20% of wild-type choline uptake activity.
- P105 (≠ R124) to S: in CMS20; decreased choline transmembrane transporter activity; no effect on localization at plasma membrane; dbSNP:rs886039766
- Y111 (≠ R130) to H: in CMS20; no effect on localization at plasma membrane
- Y175 (= Y195) to C: in CMS20; uncertain significance; dbSNP:rs1331713195
- I291 (≠ V323) to T: in CMS20; uncertain significance; dbSNP:rs375397889
- V344 (≠ I368) to L: in CMS20; uncertain significance
- R361 (≠ H385) to Q: in CMS20; decreased choline transmembrane transporter activity; no effect on localization at plasma membrane; dbSNP:rs147656110
- F418 (= F444) to V: in CMS20; uncertain significance
Sites not aligning to the query:
- 446 R → G: in CMS20; decreased choline transmembrane transporter activity; no effect on localization at plasma membrane
- 451 E→Q: Decreased choline transmembrane transporter activity, only 5% of wild-type choline uptake activity.
- 527:532 Dileucine-like motif
- 530 I→A: No change in protein internalization. No change in choline transmembrane transporter activity.
- 531 L→A: Loss of protein internalization to vesicular structures in neurons. Increased choline transmembrane transporter activity.
- 531:532 LV→AA: Decreased protein internalization; when associated with V-538. Increased choline transmembrane transporter activity; when associated with V-538.
- 532 V→A: Decreased protein internalization. Increased choline transmembrane transporter activity.
- 538 K→V: Decreased protein internalization; when associated with 531-L-V-532. Increased choline transmembrane transporter activity; when associated with 531-L-V-532.
P13866 Sodium/glucose cotransporter 1; Na(+)/glucose cotransporter 1; High affinity sodium-glucose cotransporter; Solute carrier family 5 member 1 from Homo sapiens (Human) (see 6 papers)
21% identity, 53% coverage: 173:461/549 of query aligns to 169:485/664 of P13866
- D204 (≠ Q208) mutation to A: Loss of activity.
- N248 (≠ G256) modified: carbohydrate, N-linked (GlcNAc...) asparagine; mutation to Q: Loss of N-glycosylation.
- C255 (vs. gap) modified: Disulfide link with 511
- W276 (≠ G268) to L: in GGM; about 95% reduction in activity
- T287 (≠ M283) mutation to A: Acquires D-mannose, D-fructose and L-sorbose transporter activity; when associated with L-83 and C-290.; mutation to N: Loss of D-glucose transporter activity. Has strict selectivity for D-galactose.; mutation T->S,A: Has normal D-glucose and D-galactose transporter activity.
- Y290 (≠ F286) mutation to C: Loss of D-galactose transporter activity. Has strict selectivity for D-glucose. Acquires D-mannose, D-fructose and L-sorbose transporter activity; when associated with A-287 and L-83.
- W291 (≠ T287) mutation to A: Loss of D-glucose transporter activity.
- C292 (≠ V288) to Y: in GGM; loss of activity; mutation to A: Has no effect on water permeability.
- Q295 (≠ A291) to R: in GGM; loss of activity
- R300 (vs. gap) to S: in GGM; loss of activity
- A304 (vs. gap) to V: in GGM; impairs trafficking to the plasma membrane
- K321 (≠ Y309) mutation to Q: Acquires D-mannose and D-allose transporter activity comparable to glucose and galactose.
- C345 (≠ F329) modified: Disulfide link with 351
- C351 (vs. gap) modified: Disulfide link with 345
- C355 (≠ A333) modified: Disulfide link with 361
- C361 (≠ G339) modified: Disulfide link with 355
- N363 (= N341) mutation to A: Loss of water permeation.
- L369 (= L347) to S: in GGM; loss of activity
- R379 (≠ L357) to Q: in GGM; loss of activity
- A388 (= A366) to V: in GGM; loss of activity
- S396 (≠ G374) mutation to A: Loss of activity.
- F405 (≠ V383) to S: in GGM; loss of activity
- A411 (= A389) to T: in GGM; slightly decreased activity; dbSNP:rs17683430
- G426 (≠ S405) to R: in GGM; loss of activity
- Q451 (≠ F430) mutation to A: Strong reduction in water permeation.
- L452 (≠ M431) mutation to A: Loss of water permeation.
- D454 (≠ G433) mutation to A: Has no effect on water permeation.
- Q457 (≠ F436) mutation to A: Loss of D-glucose transporter activity.; mutation to C: Strong reduction in D-glucose transporter activity.
- T460 (≠ A439) mutation to A: Loss of D-glucose transporter activity.
- V470 (vs. gap) to N: in GGM; about 90% reduction in activity; requires 2 nucleotide substitutions
Sites not aligning to the query:
- 51 N → S: in GGM; slightly decreased activity; dbSNP:rs17683011
- 67 W→A: Strong reduction in D-glucose transporter activity.
- 77 S→A: Loss of activity.
- 83 H→L: Acquires D-mannose, D-fructose and L-sorbose transporter activity; when associated with A-287 and C-290.; H→Q: Loss of D-glucose transporter activity.
- 135 R → W: in GGM; loss of activity
- 159 S → P: in GGM; loss of activity
- 166 A → T: in GGM; about 90% reduction in activity
- 191:664 natural variant: Missing (in GGM; loss of activity)
- 379:664 natural variant: Missing (in GGM; loss of activity)
- 499 R → H: in GGM; impairs trafficking to the plasma membrane; decreases the sugar affinity
- 511 modified: Disulfide link with 255
- 517 modified: Disulfide link with 522
- 522 modified: Disulfide link with 517
- 615 H → Q: in GGM; slightly decreased activity
- 641 W→A: Slightly reduced D-glucose transporter activity.
- 660:661 HA→WG: Loss of D-glucose transporter activity.
7wmvA Structure of human sglt1-map17 complex bound with lx2761 (see paper)
21% identity, 53% coverage: 173:461/549 of query aligns to 152:468/602 of 7wmvA
- binding N-[2-(dimethylamino)ethyl]-2-methyl-2-[4-[4-[[2-methyl-5-[(2S,3R,4R,5S,6R)-6-methylsulfanyl-3,4,5-tris(oxidanyl)oxan-2-yl]phenyl]methyl]phenyl]butanoylamino]propanamide: L257 (≠ S266), M266 (≠ T275), L269 (= L282), T270 (≠ M283), Y273 (≠ F286), W274 (≠ T287), F436 (≠ V432), D437 (≠ G433), Q440 (≠ F436)
Sites not aligning to the query:
- binding N-[2-(dimethylamino)ethyl]-2-methyl-2-[4-[4-[[2-methyl-5-[(2S,3R,4R,5S,6R)-6-methylsulfanyl-3,4,5-tris(oxidanyl)oxan-2-yl]phenyl]methyl]phenyl]butanoylamino]propanamide: 61, 66, 70, 81, 84, 508
Query Sequence
>BWI76_RS02085 FitnessBrowser__Koxy:BWI76_RS02085
MKKVLTALAATLPFTANAADAITGAVQRQPTNWQAIIMFLIFVALTLYITYWASKRVRSR
SDYYTAGGNITGFQNGLAIAGDFMSAASFLGISALVYTSGYDGLIYSLGFLVGWPIILFL
IAERLRNLGRYTFADVASYRLKQGPIRTLSACGSLVVVALYLIAQMVGAGKLIQLLFGLN
YHIAVVLVGVLMVLYVLFGGMLATTWVQIIKAVLLLFGASFMAFMVMKHVGFSFNNLFSE
AMAVHPKGAAIMSPGGLVKDPISALSLGLGLMFGTAGLPHILMRFFTVSDAKEARKSVFY
ATGFMGYFYILTFIIGFGAIMLVGANPAFKDAAGQLIGGNNMAAVHLADAVGGNLFLGFI
SAVAFATILAVVAGLTLAGASAVSHDLYANVFRKGATERQELKVSKITVLILGVVAILLG
ILFENQNIAFMVGLAFSIAASCNFPIILLSMYWSKLTTRGAMIGGWLGLLTAVILMVLGP
TIWVQILGHEKAIFPYEYPALFSIAVAFIGIWFFSATDNSAEGSRERELFRAQFIRSQTG
IGIEKGQAH
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SitesBLAST's Database
SitesBLAST's database includes
(1) SwissProt
entries with experimentally-supported functional features;
and (2) protein structures with bound ligands, from the
BioLip database.
by Morgan Price,
Arkin group
Lawrence Berkeley National Laboratory