SitesBLAST

SitesBLAST – Find functional sites

SitesBLAST

Comparing BWI76_RS02105 FitnessBrowser__Koxy:BWI76_RS02105 to proteins with known functional sites using BLASTp with E ≤ 0.001.

Or try Sites on a Tree, PaperBLAST, Conserved Domains, or compare to all protein structures

Found 20 (the maximum) hits to proteins with known functional sites (download)

O59010 Glutamate transporter homolog; Glt(Ph); Sodium-aspartate symporter Glt(Ph); Sodium-dependent aspartate transporter from Pyrococcus horikoshii (strain ATCC 700860 / DSM 12428 / JCM 9974 / NBRC 100139 / OT-3) (see 3 papers)
35% identity, 95% coverage: 2:418/437 of query aligns to 5:421/425 of O59010

query
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O59010

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S65 (≠ T64) mutation to V: Strongly decreased chloride conductance.
R276 (≠ A279) mutation to S: Increased rate of aspartate transport; when associated with R-395.
RSS 276:278 (≠ ASS 279:281) binding
M311 (≠ L314) mutation to A: Decreased dependence of aspartate binding on Na(+) concentration.
T314 (≠ S317) binding
V355 (= V358) binding
D394 (= D391) binding
M395 (= M392) mutation to R: Increased rate of aspartate transport; when associated with S-276.
R397 (= R394) mutation to A: Strongly decreased affinity for aspartate.
N401 (= N398) binding
D405 (≠ N402) mutation to N: Strongly decreased affinity for aspartate.

6zl4A The structure of glutamate transporter homologue glttk in complex with the photo switchable compound (cis) (see paper)
35% identity, 94% coverage: 11:420/437 of query aligns to 9:420/424 of 6zl4A

query
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6zl4A

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binding decyl-beta-d-maltopyranoside: Y190 (≠ F198), L191 (≠ K199), G194 (≠ H202), G195 (≠ M203), Q198 (≠ R206), R282 (= R287), E286 (= E293)
binding (2~{S},3~{S})-2-azanyl-3-[[4-[2-(4-methoxyphenyl)hydrazinyl]phenyl]methoxy]butanedioic acid: R271 (≠ A279), S272 (= S280), S273 (= S281), M307 (≠ L314), T310 (≠ S317), V351 (= V358), P352 (= P359), G353 (= G360), A354 (≠ V361), G355 (≠ S362), D391 (= D391), R394 (= R394), T395 (= T395), N398 (= N398)

Sites not aligning to the query:

binding decyl-beta-d-maltopyranoside: 3

6zgbA Glutamate transporter homologue glttk in complex with a photo cage compound (see paper)
35% identity, 94% coverage: 11:420/437 of query aligns to 10:421/425 of 6zgbA

query
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6zgbA

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L

A

A

M

T

V

L

L

G

Q

S

S

V

V

G

G

I

L

P

D

L

L

E

T

-

P

-

G

-

S

-

P

-

V

-

A

-

L

G

A

L

Y

A

A

F

M

I

I

A

L

G

G

V

I

D

D

R

A

I

I

L

L

D
|
D

M

M

A

G

R
|
R

T
|
T

A

M

L

V

N

N

V

V

V

T

G

G

N

D

A

L

L

A

A

G

V

T

L

V

V

I

I

V

A

A

K

K

W

T

E

E

H

K

K

E

F

L

D

D

R

E

K

S

K

K

binding (2~{S},3~{S})-2-azanyl-3-[(2-nitrophenyl)methoxy]butanedioic acid: R272 (≠ A279), S273 (= S280), S274 (= S281), M308 (≠ L314), T311 (≠ S317), D392 (= D391), R395 (= R394), T396 (= T395)

5e9sA Crystal structure of substrate-bound glutamate transporter homologue glttk (see paper)
34% identity, 96% coverage: 1:420/437 of query aligns to 2:423/427 of 5e9sA

query
sites
5e9sA

M

L

K

R

K

Y

T

L

K

D

V

Y

S

P

M

V

A

L

W

W

Q

K

I

I

L

L

L

W

A

G

L

L

V

V

L

L

G

G

I

A

L

V

L

F

G

G

-

L

-

I

-

A

S

G

Y

H

L

F

H

G

Y

Y

H

A

A

G

E

A

S

V

R

K

D

T

W

Y

L

I

I

-

S

-

N

-

L

-

T

K

P

P

A

F

G

G

D

D

I

L

F

F

I

V

H

R

L

L

I

L

K

K

M

M

I

L

V

V

V

M

P

P

I

I

V

V

I

L

S

A

T

S

L

L

V

V

G

G

I

A

A

A

G

S

V

I

G

S

D

P

A

A

K

R

Q

-

L

L

G

G

R

R

I

V

G

G

A

V

K

K

T

I

I

V

Y

Y

F
x
Y

E

L

V

A

I
x
T

T
x
S

T

A

V

M

A

A

I

V

V

F

L

F

G

G

I

L

T

I

L

V

A

G

N

R

V

L

F

F

Q

N

P

V

G

G

S

A

G

N

I

V

D

N

M

L

S

G

Q

S

L

-

A

-

V

-

D

-

I

-

S

-

K

-

Y

-

Q

-

N

G

T

T

T

G

A

K

E

A

V

I

Q

E

S

A

H

Q

A

P

H

P

G

S

L

L

M

V

G

Q

T

T

I

L

L

L

S

N

L

I

V

V

P

P

T

T

N

N

I

P

V

F

A

A

S

S

M

L

A

A

K

K

G

G

D

E

M

V

L

L

P

P

I

V

I

I

F

F

S

A

V

I

L

I

F

L

G

G

L

I

G

A

L

I

S

T

S

Y

L

L

P

M

A

N

T

R

H

N

R

E

E

E

-

R

-

V

-

R

-

K

-

S

-

A

-

E

P

T

L

L

V

L

T

R

V

V

F

F

R

D

S

G

I

L

S

A

E

E

T

A

M

M

F

Y

K
x
L

V

I

T

V

H
x
G

M
x
G

V

V

M

M

R

Q

Y
|
Y

A

A

P

P

V

I

G

G

V

V

F

F

A

A

L

L

I

I

S

A

V

Y

T

V

V

M

A

A

T

E

F

Q

G

G

F

V

A

R

S

V

L

V

W

G

P

P

L

L

A

A

K

K

L

V

V

V

I

G

L

A

V

V

Y

Y

F

T

A

G

I

L

L

F

F

L

F

Q

A

I

L

V

V

I

V

T

L

Y

G

F

I

I

V

L

A

L

R

K

V

V

C

F

G

G

L

I

S

D

I

P

W

I

T

K

L

F

I

I

R

R

I

K

L

A

K

K

D

D

E

A

L

M

I

I

L

T

A

A

Y

F

S

V

T

T

A
x
R

S
|
S

E

S

S

G

V

T

L

L

P

P

-

V

-

T

-

M

R
|
R

I

V

I

A

E

E

K

-

M

-

E

E

A

E

Y

M

G

G

A

V

P

D

A

K

S

G

I

I

T

F

S

S

F

F

V

T

V

L

P

P

T

L

G
|
G

Y
x
A

S
x
T

F
x
I

N
|
N

L
x
M

D
|
D

G

G

S
x
T

T

A

L

L

Y

Y

Q

Q

S

G

I

V

A

T

A

V

I

L

F

F

I

V

A

A

Q

N

L

A

Y

I

G

G

I

H

D

P

L

L

S

T

I

L

W

G

Q

Q

E

Q

I

L

T

V

L

V

V

V

L

L

T

T

L

A

M

V

V

L

T

A

S
|
S

K
x
I

G
|
G

I
x
T

A
|
A

G
|
G

V
|
V

P
|
P

G
|
G

V
x
A

S
x
G

F

A

V

I

V

M

L

L

L

A

A

M

T

V

L

L

G

Q

S

S

V

V

G

G

I

L

P

D

L

L

E

T

-

P

-

G

-

S

-

P

-

V

-

A

-

L

G

A

L

Y

A

A

F

M

I

I

A

L

G

G

V

I

D

D

R

A

I

I

L

L

D
|
D

M

M

A

G

R
|
R

T
|
T

A

M

L

V

N
|
N

V
|
V

V

T

G

G

N
x
D

A

L

L

A

A

G

V

T

L

V

V

I

I

V

A

A

K

K

W

T

E

E

H

K

K

E

F

L

D

D

R

E

K

S

K

K

binding aspartic acid: R274 (≠ A279), S275 (= S280), S276 (= S281), T313 (≠ S317), T351 (≠ I355), A352 (= A356), G353 (= G357), V354 (= V358), P355 (= P359), G356 (= G360), A357 (≠ V361), G358 (≠ S362), D394 (= D391), R397 (= R394), T398 (= T395), N401 (= N398)
binding decyl-beta-d-maltopyranoside: L194 (≠ K199), G197 (≠ H202), G198 (≠ M203), Y202 (= Y207), R285 (= R287)
binding sodium ion: Y87 (≠ F89), T90 (≠ I92), S91 (≠ T93), S276 (= S281), G305 (= G309), A306 (≠ Y310), T307 (≠ S311), I308 (≠ F312), N309 (= N313), N309 (= N313), M310 (≠ L314), M310 (≠ L314), D311 (= D315), S348 (= S352), I349 (≠ K353), G350 (= G354), T351 (≠ I355), N401 (= N398), V402 (= V399), D405 (≠ N402)

6xwnB Structure of glutamate transporter homologue glttk in the presence of tboa inhibitor (see paper)
34% identity, 96% coverage: 1:420/437 of query aligns to 2:423/426 of 6xwnB

query
sites
6xwnB

M

L

K

R

K

Y

T

L

K

D

V

Y

S

P

M

V

A

L

W

W

Q

K

I

I

L

L

L

W

A

G

L

L

V

V

L

L

G

G

I

A

L

V

L

F

G

G

-

L

-

I

-

A

S

G

Y

H

L

F

H

G

Y

Y

H

A

A

G

E

A

S

V

R

K

D

T

W

Y

L

I

I

-

S

-

N

-

L

-

T

K

P

P

A

F

G

G

D

D

I

L

F

F

I

V

H

R

L

L

I

L

K

K

M

M

I

L

V

V

V

M

P

P

I

I

V

V

I

L

S

A

T

S

L

L

V

V

G

G

I

A

A

A

G

S

V

I

G

S

D

P

A

A

K

R

Q

-

L

L

G

G

R

R

I

V

G

G

A

V

K

K

T

I

I

V

Y

Y

F

Y

E

L

V

A

I

T

T

S

T

A

V

M

A

A

I

V

V

F

L

F

G

G

I

L

T

I

L

V

A

G

N

R

V

L

F

F

Q

N

P

V

G

G

S

A

G

N

I

V

D

N

M

L

S

G

Q

S

L

-

A

-

V

-

D

-

I

-

S

-

K

-

Y

-

Q

-

N

G

T

T

T

G

A

K

E

A

V

I

Q

E

S

A

H

Q

A

P

H

P

G

S

L

L

M

V

G

Q

T

T

I

L

L

L

S

N

L

I

V

V

P

P

T

T

N

N

I

P

V

F

A

A

S

S

M

L

A

A

K

K

G

G

D

E

M

V

L

L

P

P

I

V

I

I

F

F

S

A

V

I

L

I

F

L

G

G

L

I

G

A

L

I

S

T

S

Y

L

L

P

M

A

N

T

R

H

N

R

E

E

E

-

R

-

V

-

R

-

K

-

S

-

A

-

E

P

T

L

L

V

L

T

R

V

V

F

F

R

D

S

G

I

L

S

A

E

E

T

A

M

M

F

Y

K

L

V

I

T

V

H

G

M

G

V

V

M

M

R

Q

Y

Y

A

A

P

P

V

I

G

G

V

V

F

F

A

A

L

L

I

I

S

A

V

Y

T

V

V

M

A

A

T

E

F

Q

G

G

F

V

A

R

S

V

L

V

W

G

P

P

L

L

A

A

K

K

L

V

V

V

I

G

L

A

V

V

Y

Y

F

T

A

G

I

L

L

F

F

L

F

Q

A

I

L

V

V

I

V

T

L

Y

G

F

I

I

V

L

A

L

R

K

V

V

C

F

G

G

L

I

S

D

I

P

W

I

T

K

L

F

I

I

R

R

I

K

L

A

K

K

D

D

E

A

L

M

I

I

L

T

A

A

Y

F

S

V

T

T

A
x
R

S
|
S

E

S

S

G

V

T

L

L

P

P

-

V

-

T

-

M

R

R

I

V

I

A

E

E

K

-

M

-

E

E

A

E

Y

M

G

G

A

V

P

D

A

K

S

G

I

I

T

F

S

S

F

F

V

T

V

L

P

P

T

L

G

G

Y

A

S
x
T

F

I

N

N

L
x
M

D

D

G

G

S
x
T

T

A

L

L

Y

Y

Q

Q

S

G

I

V

A

T

A

V

I

L

F

F

I

V

A

A

Q

N

L

A

Y

I

G

G

I

H

D

P

L

L

S

T

I

L

W

G

Q

Q

E

Q

I

L

T

V

L

V

V

V

L

L

T

T

L

A

M

V

V

L

T

A

S

S

K

I

G

G

I

T

A

A

G

G

V

V

P

P

G

G

V

A

S
x
G

F

A

V

I

V

M

L

L

L

A

A

M

T

V

L

L

G

Q

S

S

V

V

G

G

I

L

P

D

L

L

E

T

-

P

-

G

-

S

-

P

-

V

-

A

-

L

G

A

L

Y

A

A

F

M

I

I

A

L

G

G

V

I

D

D

R

A

I

I

L

L

D
|
D

M

M

A

G

R
|
R

T
|
T

A

M

L

V

N

N

V

V

V

T

G

G

N

D

A

L

L

A

A

G

V

T

L

V

V

I

I

V

A

A

K

K

W

T

E

E

H

K

K

E

F

L

D

D

R

E

K

S

K

K

binding (3s)-3-(benzyloxy)-l-aspartic acid: R274 (≠ A279), S275 (= S280), S276 (= S281), T307 (≠ S311), M310 (≠ L314), T313 (≠ S317), G358 (≠ S362), D394 (= D391), R397 (= R394), T398 (= T395)

6x14A Inward-facing state of the glutamate transporter homologue gltph in complex with tfb-tboa (see paper)
35% identity, 94% coverage: 2:413/437 of query aligns to 2:413/413 of 6x14A

query
sites
6x14A

K

R

K

K

K
x
Y

T

I

K

E

V

Y

S

P

M

V

A

L

W

Q

Q

K

I
|
I

L

L

L

I

A

G

L

L

V

I

L

L

G

G

-

A

-

I

-

V

-

G

I

L

L

I

L

L

G

G

S

H

Y

Y

L

G

H

Y

Y

A

H

H

A

A

E

-

S

-

R

-

D

-

W

-

L

-

I

V

S

H

N

T

L

Y

L

V

T

K

P

P

A

F

G

G

D

D

I

L

F
|
F

I

V

H

R

L

L

I

L

K

C

M

M

I

L

V

V

V

M

P

P

I

I

V

V

I

F

S

A

T

S

L

L

V

V

G
|
G

I
x
A

A

A

G

S

V
x
I

G

S

D

P

A

A

K

R

Q

-

L

L

G

G

R

R

I

V

G

G

A

V

K

K

T

I

I
x
V

I

V

Y

Y

F

Y

E

L

V

L

I

T

T

S

T

A

V

F

A

A

I

V

V

T

L

L

G

G

I

I

T

I

L

M

A

A

N

R

V

L

F

F

Q

N

P

P

G

G

S

A

G

G

I

I

D

H

M

L

S

-

Q

-

L

-

A

-

A

A

V

V

D

G

I

G

S

Q

K

Q

Y

F

Q

Q

N

P

T

H

T

Q

A

A

E

P

V

P

Q

L

S

V

H

H

A

-

H

-

G

-

L

-

M

-

G

-

T

I

I

L

L

L

S

D

L

I

V

V

P

P

T

T

N

N

I

P

V

F

A

G

S

A

M

L

A

A

K

N

G

G

D

Q

M

V

L

L

P

P

I

T

I

I

F

F

S

A

V

I

L
x
I

F

L

G

G

L

I

G

A

L

I

S

T

S
x
Y

L

L

P

M

A

N

T

S

H

E

R

N

E

E

P

K

-

V

-

R

-

K

-

S

-

A

-

E

-

T

L

L

V

L

T

D

V

A

F

I

R

N

S

G

I

L

S

A

E

E

T

A

M

M

F

Y

K
|
K

V
x
I

T

V

H
x
N

M
x
G

V

V

M
|
M

R
x
Q

Y
|
Y

A

A

P

P

V

I

G

G

V

V

F

F

A

A

L

L

I

I

S

A

V

Y

T

V

V

M

A

A

T

E

F

Q

G

G

F

V

A

H

S

V

L

V

W

G

P

E

L

L

A

A

K

K

L

V

V

T

I

A

L

A

V

V

Y

Y

F

V

A

G

I

L

L

T

F

L

F

Q

A

I

L

L

V

L

V

V

L

Y

G

F

I

V

V

L

A

L

R

K

V

I

C

Y

G

G

L

I

S

D

I

P

W

I

T

S

L

F

I

I

R

K

I

H

L

A

K

K

D

D

E

A

L

M

I

L

L

T

A

A

Y

F

S

V

T

T

A
x
R

S
|
S

E

S

S

G

V

T

L

L

P

P

R

V

I

T

I

M

E

R

K

V

M

A

E

K

A

E

Y

M

G

G

A

I

P

S

A

E

S

G

I

I

T

Y

S

S

F

F

V

T

V

L

P

P

T
x
L

G

G

Y

A

S
x
T

F
x
I

N

N

L
x
M

D

D

G

G

S
x
T

T

A

L

L

Y

Y

Q

Q

S

G

I

V

A

A

A

T

I

F

F

F

I

I

A

A

Q

N

L

A

Y

L

G

G

I

S

D

H

L

L

S

T

I

V

W

G

Q

Q

E

Q

I

L

T

T

L

I

V

V

L

L

T

T

L

A

M

V

V

L

T

A

S

S

K
x
I

G

G

I

T

A
|
A

G

G

V

V

P
|
P

G

G

V

A

S
x
G

F
x
A

V

I

V

M

L

L

L

C

A

M

T

V

L

L

G

H

S

S

V

V

G

G

I

L

P

P

L

L

-

T

-

D

-

P

-

N

-

V

-

A

E

A

G

A

L

Y

A

A

F

M

I

I

A
x
L

G

G

V

I

D

D

R

A

I

I

L

L

D
|
D

M

M

A

G

R
|
R

T
|
T

A

M

L

V

N

N

V

V

V

T

G

G

N

D

A

L

L

T

A

G

V

T

L

A

V

I

I

V

A

A

K

K

W

T

E

E

binding [(2~{R})-1-[2-azanylethoxy(oxidanyl)phosphoryl]oxy-3-hexadecanoyloxy-propan-2-yl] (~{Z})-octadec-9-enoate: Y4 (≠ K4), G66 (= G68), A67 (≠ I69), I70 (≠ V72), V83 (≠ I86), I157 (≠ L170), Y164 (≠ S177), K193 (= K199), I194 (≠ V200), N196 (≠ H202), G197 (≠ M203), Q200 (≠ R206), Y201 (= Y207), L302 (≠ T308), T305 (≠ S311), I306 (≠ F312), I347 (≠ K353), A350 (= A356)
binding (2~{S},3~{S})-2-azanyl-3-[[3-[[4-(trifluoromethyl)phenyl]carbonylamino]phenyl]methoxy]butanedioic acid: I13 (= I13), F47 (= F49), M199 (= M205), R273 (≠ A279), S274 (= S280), S275 (= S281), M308 (≠ L314), T311 (≠ S317), P353 (= P359), G356 (≠ S362), A357 (≠ F363), L384 (≠ A384), D391 (= D391), R394 (= R394), T395 (= T395)

Sites not aligning to the query:

binding [(2~{R})-1-[2-azanylethoxy(oxidanyl)phosphoryl]oxy-3-hexadecanoyloxy-propan-2-yl] (~{Z})-octadec-9-enoate: 1

6x15A Inward-facing state of the glutamate transporter homologue gltph in complex with l-aspartate and sodium ions (see paper)
35% identity, 94% coverage: 2:413/437 of query aligns to 5:416/419 of 6x15A

query
sites
6x15A

K

R

K
|
K

K
x
Y

T
x
I

K

E

V

Y

S

P

M

V

A

L

W

Q

Q
x
K

I

I

L

L

L
x
I

A

G

L

L

V

I

L

L

G

G

-

A

-

I

-

V

-

G

I

L

L

I

L

L

G

G

S

H

Y

Y

L

G

H

Y

Y

A

H

H

A

A

E

-

S

-

R

-

D

-

W

-

L

-

I

V

S

H

N

T

L
x
Y

L

V

T

K

P
|
P

A
x
F

G

G

D

D

I
x
L

F

F

I

V

H

R

L
|
L

I

L

K

C

M

M

I

L

V

V

V

M

P

P

I

I

V

V

I

F

S

A

T

S

L

L

V

V

G

G

I

A

A

A

G

S

V
x
I

G

S

D
x
P

A

A

K

R

Q

-

L

L

G

G

R

R

I

V

G

G

A

V

K
|
K

T

I

I

V

Y

Y

F
x
Y

E
x
L

V
x
L

I
x
T

T
x
S

T

A

V
x
F

A

A

I

V

V

T

L

L

G

G

I

I

T

I

L

M

A

A

N

R

V

L

F

F

Q

N

P

P

G

G

S

A

G

G

I

I

D

H

M

L

S

-

Q

-

L

-

A

-

A

A

V

V

D

G

I

G

S

Q

K

Q

Y

F

Q

Q

N

P

T

H

T

Q

A

A

E

P

V

P

Q
x
L

S
x
V

H

H

A

-

H

-

G

-

L

-

M

-

G

-

T
x
I

I
x
L

L

L

S

D

L
x
I

V

V

P

P

T

T

N

N

I

P

V

F

A

G

S

A

M

L

A

A

K

N

G

G

D

Q

M

V

L
|
L

P

P

I

T

I

I

F
|
F

F

F

S

A

V
x
I

L

I

F

L

G

G

L
x
I

G
x
A

L

I

S

T

S
x
Y

L

L

P

M

A

N

T

S

H

E

R

N

E

E

P

K

-

V

-

R

-

K

-

S

-

A

-

E

-

T

L

L

V

L

T

D

V

A

F

I

R

N

S

G

I

L

S

A

E

E

T

A

M

M

F

Y

K
|
K

V
x
I

T

V

H
x
N

M
x
G

V

V

M

M

R

Q

Y
|
Y

A

A

P

P

V
x
I

G

G

V

V

F
|
F

A

A

L

L

I

I

S

A

V

Y

T

V

V

M

A

A

T

E

F

Q

G

G

F

V

A

H

S

V

L

V

W

G

P

E

L

L

A

A

K

K

L

V

V

T

I

A

L

A

V

V

Y

Y

F

V

A

G

I

L

L

T

F

L

F

Q

A

I

L

L

V

L

V

V

L

Y

G

F

I

V

V
x
L

A

L

R

K

V
x
I

C
x
Y

G

G

L

I

S

D

I

P

W

I

T

S

L

F

I
|
I

R

K

I

H

L

A

K
|
K

D

D

E

A

L
x
M

I

L

L

T

A

A

Y

F

S

V

T

T

A
x
R

S
|
S

E

S

S

G

V

T

L

L

P

P

R

V

I

T

I

M

E

R

K

V

M

A

E

K

A

E

Y

M

G

G

A

I

P

S

A

E

S

G

I

I

T

Y

S

S

F

F

V

T

V

L

P

P

T

L

G
|
G

Y
x
A

S
x
T

F

I

N
|
N

L
x
M

D
|
D

G

G

S
x
T

T

A

L

L

Y

Y

Q

Q

S

G

I

V

A

A

A

T

I

F

F

F

I

I

A

A

Q

N

L

A

Y

L

G

G

I

S

D

H

L

L

S
x
T

I
x
V

W
x
G

Q
|
Q

E

Q

I
x
L

T
|
T

L

I

V

V

L
|
L

T

T

L

A

M

V

V

L

T

A

S
|
S

K
x
I

G
|
G

I
x
T

A

A

G
|
G

V
|
V

P

P

G
|
G

V
x
A

S
x
G

F

A

V

I

V

M

L

L

L

C

A

M

T

V

L

L

G

H

S
|
S

V

V

G

G

I

L

P

P

L

L

-

T

-

D

-

P

-

N

-

V

-

A

E

A

G

A

L

Y

A

A

F

M

I

I

A

L

G

G

V

I

D

D

R

A

I

I

L

L

D
|
D

M

M

A

G

R
|
R

T
|
T

A
x
M

L

V

N
|
N

V
|
V

V

T

G

G

N
x
D

A

L

L

T

A

G

V

T

L

A

V

I

I

V

A

A

K

K

W

T

E

E

binding [(2~{R})-1-[2-azanylethoxy(oxidanyl)phosphoryl]oxy-3-hexadecanoyloxy-propan-2-yl] (~{Z})-octadec-9-enoate: K6 (= K3), Y7 (≠ K4), I8 (≠ T5), K15 (≠ Q12), I18 (≠ L15), Y42 (≠ L41), P45 (= P44), F46 (≠ A45), F46 (≠ A45), F46 (≠ A45), L49 (≠ I48), L49 (≠ I48), L53 (= L52), I73 (≠ V72), P75 (≠ D74), K84 (= K84), L90 (≠ E90), L91 (≠ V91), L91 (≠ V91), F95 (≠ V95), L130 (≠ Q134), V131 (≠ S135), I133 (≠ T143), L134 (≠ I144), I137 (≠ L147), L152 (= L162), F156 (= F166), I159 (≠ V169), I163 (≠ L173), I163 (≠ L173), I163 (≠ L173), A164 (≠ G174), Y167 (≠ S177), Y167 (≠ S177), K196 (= K199), I197 (≠ V200), N199 (≠ H202), G200 (≠ M203), Y204 (= Y207), I207 (≠ V210), F210 (= F213), L250 (≠ V253), I253 (≠ V256), Y254 (≠ C257), Y254 (≠ C257), I262 (= I265), K266 (= K269), M269 (≠ L272), T334 (≠ S337), V335 (≠ I338), G336 (≠ W339), Q337 (= Q340), L339 (≠ I342), T340 (= T343), L343 (= L346), S369 (= S372), M399 (≠ A396)
binding aspartic acid: R276 (≠ A279), S277 (= S280), S278 (= S281), T314 (≠ S317), T352 (≠ I355), G354 (= G357), V355 (= V358), G357 (= G360), A358 (≠ V361), G359 (≠ S362), D394 (= D391), R397 (= R394), T398 (= T395), N401 (= N398)
binding sodium ion: Y89 (≠ F89), T92 (≠ I92), S93 (≠ T93), G306 (= G309), A307 (≠ Y310), T308 (≠ S311), N310 (= N313), N310 (= N313), M311 (≠ L314), D312 (= D315), S349 (= S352), I350 (≠ K353), G351 (= G354), T352 (≠ I355), N401 (= N398), V402 (= V399), D405 (≠ N402)

Sites not aligning to the query:

binding [(2~{R})-1-[2-azanylethoxy(oxidanyl)phosphoryl]oxy-3-hexadecanoyloxy-propan-2-yl] (~{Z})-octadec-9-enoate: 1, 3, 4, 4

2nwwA Crystal structure of gltph in complex with tboa (see paper)
35% identity, 92% coverage: 13:413/437 of query aligns to 11:407/407 of 2nwwA

query
sites
2nwwA

I

L

L

I

L

L

A

G

L

A

V

I

L

V

G

G

I

L

L

I

L

L

G

G

S

H

Y

Y

L

G

H

Y

Y

A

H

H

A

A

E

-

S

-

R

-

D

-

W

-

L

-

I

V

S

H

N

T

L

Y

L

V

T

K

P

P

A

F

G

G

D

D

I

L

F

F

I

V

H

R

L

L

I

L

K

K

M

M

I

L

V

V

V

M

P

P

I

I

V

V

I

F

S

A

T

S

L

L

V

V

G

G

I

A

A

A

G

S

V

I

G

S

D

P

A

A

K

R

Q

-

L

L

G

G

R

R

I

V

G

G

A

V

K

K

T

I

I

V

Y

Y

F

Y

E

L

V

L

I

T

T

S

T

A

V

F

A

A

I

V

V

T

L

L

G

G

I

I

T

I

L

M

A

A

N

R

V

L

F

F

Q

N

P

P

G

G

S

A

G

G

I

I

D

H

M

L

S

-

Q

-

L

-

A

-

A

A

V

V

D

G

I

G

S

Q

K

Q

Y

F

Q

Q

N

P

T

H

T

Q

A

A

E

P

V

P

Q

L

S

V

H

H

A

-

H

-

G

-

L

-

M

-

G

-

T

I

I

L

L

L

S

D

L

I

V

V

P

P

T

T

N

N

I

P

V

F

A

G

S

A

M

L

A

A

K

N

G

G

D

Q

M

V

L

L

P

P

I

T

I

I

F

F

S

A

V

I

L

I

F

L

G

G

L

I

G

A

L

I

S

T

S

Y

L

L

P

M

A

N

T

S

H

E

R

N

E

E

P

K

-

V

-

R

-

K

-

S

-

A

-

E

-

T

L

L

V

L

T

D

V

A

F

I

R

N

S

G

I

L

S

A

E

E

T

A

M

M

F

Y

K

K

V

I

T

V

H

N

M

G

V

V

M

M

R

Q

Y

Y

A

A

P

P

V

I

G

G

V

V

F

F

A

A

L

L

I

I

S

A

V

Y

T

V

V

M

A

A

T

E

F

Q

G

G

F

V

A

H

S

V

L

V

W

G

P

E

L

L

A

A

K

K

L

V

V

T

I

A

L

A

V

V

Y

Y

F

V

A

G

I

L

L

T

F

L

F

Q

A

I

L

L

V

L

V

V

L

Y

G

F

I

V

V

L

A

L

R

K

V

I

C

Y

G

G

L

I

S

D

I

P

W

I

T

S

L

F

I

I

R

K

I

H

L

A

K

K

D

D

E

A

L

M

I

L

L

T

A

A

Y

F

S

V

T

T

A
x
R

S
|
S

E

S

S

G

V

T

L

L

P

P

R

V

I

T

I

M

E

R

K

V

M

A

E

K

A

E

Y

M

G

G

A

I

P

S

A

E

S

G

I

I

T

Y

S

S

F

F

V

T

V

L

P

P

T

L

G

G

Y

A

S

T

F

I

N

N

L

M

D

D

G

G

S
x
T

T

A

L

L

Y

Y

Q

Q

S

G

I

V

A

C

A

T

I

F

F

F

I

I

A

A

Q

N

L

A

Y

L

G

G

I

S

D

H

L

L

S

T

I

V

W

G

Q

Q

E

Q

I

L

T

T

L

I

V

V

L

L

T

T

L

A

M

V

V

L

T

A

S

S

K

I

G

G

I

T

A

A

G

G

V

V

P

P

G
|
G

V
x
A

S
x
G

F

A

V

I

V

M

L

L

L

A

A

M

T

V

L

L

G

H

S

S

V

V

G

G

I

L

P

P

L

L

-

T

-

D

-

P

-

N

-

V

-

A

E

A

G

A

L

Y

A

A

F

M

I

I

A

L

G

G

V

I

D

D

R

A

I

I

L

L

D
|
D

M

M

A

G

R
|
R

T
|
T

A

M

L

V

N
|
N

V

V

V

T

G

G

N

D

A

L

L

T

A

G

V

T

L

A

V

I

I

V

A

A

K

K

W

T

E

E

binding (3s)-3-(benzyloxy)-l-aspartic acid: R267 (≠ A279), S268 (= S280), S269 (= S281), T305 (≠ S317), G348 (= G360), A349 (≠ V361), G350 (≠ S362), D385 (= D391), R388 (= R394), T389 (= T395), N392 (= N398)

6r7rA Crystal structure of the glutamate transporter homologue glttk in complex with d-aspartate (see paper)
35% identity, 95% coverage: 11:424/437 of query aligns to 5:416/416 of 6r7rA

query
sites
6r7rA

W

W

Q

K

I

I

L

L

L

W

A

G

L

L

V

V

L

L

G

G

I

A

L

V

L

F

G

G

-

L

-

I

-

A

S

G

Y
x
H

L

F

H

G

Y

Y

H

A

A

G

E

A

S

V

R

K

D

T

W

Y

L

I

I

-

S

-

N

-

L

-

T

K

P

P

A

F

G

G

D

D

I

L

F

F

I

V

H

R

L

L

I

L

K

K

M

M

I

L

V

V

V

M

P

P

I

I

V

V

I

L

S

A

T

S

L

L

V

V

G

G

I

A

A

A

G

S

V

I

G

S

D

P

A

A

K

R

Q

-

L

L

G

G

R

R

I

V

G

G

A

V

K

K

T

I

I

V

Y

Y

F

Y

E

L

V

A

I

T

T

S

T

A

V

M

A

A

I

V

V

F

L

F

G

G

I

L

T

I

L

V

A

G

N

R

V

L

F

F

Q

N

P

V

G

G

S

A

G

N

I

V

D

N

M

L

S

G

Q

S

L

-

A

-

V

-

D

-

I

-

S

-

K

-

Y

-

Q

-

N

G

T

T

T

G

A

A

E

Q

V

P

Q

P

S

S

H

-

A

-

H

-

G

-

L

L

M

V

G

Q

T

T

I

L

L

L

S

N

L

I

V

V

P

P

T

T

N

N

I

P

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F

A

A

S

S

M

L

A

A

K

K

G

G

D

E

M

V

L

L

P

P

I

V

I

I

F

F

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A

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I

L

I

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G

A

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Y

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A

N

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N

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-

R

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K

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S

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A

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P

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L

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F

F

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G

I

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A

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A

M

M

F

Y

K

L

V

I

T

V

H

G

M

G

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V

M

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Y

Y

A

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I

G

G

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F

F

A

A

L

L

I

I

S

A

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Y

T

V

V

M

A

A

T

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F

Q

G

G

F

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A

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x
V

L
x
V

W

G

P

P

L

L

A
|
A

K
|
K

L

V

V

V

I
x
G

L

A

V

V

Y

Y

F

T

A

G

I

L

L

F

F

L

F

Q

A

I

L

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V

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L

Y

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L

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L

A

K

K

D

D

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A

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M

I

I

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A

A

Y

F

S

V

T

T

A
x
R

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|
S

E

S

S

G

V

T

L

L

P

P

-

V

-

T

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M

R

R

I

V

I

A

E

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K

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M

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E

A

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Y

M

G

G

A

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D

A

K

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I

T

F

S

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F

F

V

T

V

L

P

P

T

L

G

G

Y

A

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T

F

I

N

N

L
x
M

D

D

G

G

S
x
T

T

A

L

L

Y

Y

Q

Q

S

G

I

V

A

T

A

V

I

L

F

F

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N

L

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L

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M

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V

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K

I

G

G

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x
T

A
|
A

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|
G

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|
V

P

P

G

G

V

A

S
x
G

F

A

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I

V

M

L

L

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A

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L

L

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D

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-

P

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A

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Y

A

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F

M

I

I

A

L

G

G

V

I

D

D

R

A

I

I

L

L

D
|
D

M

M

A

G

R
|
R

T
|
T

A

M

L

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N
|
N

V

V

V

T

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G

N

D

A

L

L

A

A

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T

L

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V

I

I

V

A

A

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K

W

T

E

E

H

K

K

E

F

L

D

D

R

E

K

S

K

K

A

W

L

I

A

S

Y

H

binding d-aspartic acid: R263 (≠ A279), S264 (= S280), S265 (= S281), M299 (≠ L314), T302 (≠ S317), T340 (≠ I355), A341 (= A356), G342 (= G357), V343 (= V358), G347 (≠ S362), D383 (= D391), R386 (= R394), T387 (= T395), N390 (= N398)
binding decyl-beta-d-maltopyranoside: H23 (≠ Y26), V211 (≠ S227), V212 (≠ L228), A216 (= A232), K217 (= K233), G220 (≠ I236)

6bauA Crystal structure of gltph r397c in complex with l-cysteine (see paper)
35% identity, 92% coverage: 11:413/437 of query aligns to 6:408/408 of 6bauA

query
sites
6bauA

W

Q

Q

K

I

I

L

L

L

I

A

G

L

L

V

I

L

L

G

G

I

A

L

I

L

V

G

G

S

L

Y

I

L

L

-

G

H

H

Y

Y

-

G

H

Y

A

A

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D

S

A

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D

-

W

-

L

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I

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N

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Y

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A

F

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G

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D

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K

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M

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K

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x
R

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|
S

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N

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M

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|
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|
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|
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x
G

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|
D

M

M

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C

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|
T

A

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L

V