SitesBLAST

SitesBLAST – Find functional sites

SitesBLAST

Comparing BWI76_RS02160 FitnessBrowser__Koxy:BWI76_RS02160 to proteins with known functional sites using BLASTp with E ≤ 0.001.

Or try Sites on a Tree, PaperBLAST, Conserved Domains, or compare to all protein structures

Found 20 (the maximum) hits to proteins with known functional sites (download)

P04983 Ribose import ATP-binding protein RbsA; EC 7.5.2.7 from Escherichia coli (strain K12) (see paper)
41% identity, 98% coverage: 6:507/510 of query aligns to 5:492/501 of P04983

query
sites
P04983

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K43 (= K44) mutation to R: Loss of transport.

4ymuJ Crystal structure of an amino acid abc transporter complex with arginines and atps (see paper)
33% identity, 43% coverage: 13:232/510 of query aligns to 9:223/240 of 4ymuJ

query
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4ymuJ

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binding adenosine-5'-triphosphate: F11 (= F15), V16 (≠ A20), S36 (≠ N40), G37 (= G41), S38 (≠ A42), G39 (= G43), K40 (= K44), S41 (= S45), T42 (= T46), E162 (= E171), H194 (= H203)
binding magnesium ion: S41 (= S45), E162 (= E171)

4u00A Crystal structure of ttha1159 in complex with adp (see paper)
29% identity, 45% coverage: 4:232/510 of query aligns to 1:223/241 of 4u00A

query
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4u00A

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binding adenosine-5'-diphosphate: F12 (= F15), V17 (≠ A20), S37 (≠ N40), G38 (= G41), S39 (≠ A42), G40 (= G43), K41 (= K44), S42 (= S45), T43 (= T46)

7w79A Heme exporter hrtba in complex with mn-amppnp (see paper)
32% identity, 41% coverage: 18:224/510 of query aligns to 21:216/216 of 7w79A

query
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7w79A

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binding adenosine-5'-triphosphate: S43 (≠ N40), G44 (= G41), S45 (≠ A42), G46 (= G43), K47 (= K44), S48 (= S45), T49 (= T46)
binding manganese (ii) ion: E30 (≠ T27), S48 (= S45), Q88 (= Q87), E163 (= E171), H196 (= H203)

Sites not aligning to the query:

7w78A Heme exporter hrtba in complex with mg-amppnp (see paper)
32% identity, 41% coverage: 18:224/510 of query aligns to 21:216/218 of 7w78A

query
sites
7w78A

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-

D

E

K

G

L

L

D

D

H

A

K

T

L

S

A

T

A

R

Q

R

L

E

G

H

I

I

G

G

I

F

I

V

Y

F

Q
|
Q

E

Q

L

P

S

N

V

L

I

L

D

G

E

S

L

L

T

T

V

A

L

R

E

E

N

Q

L

L

Y

L

I

I

G

T

R

D

H

H

L

L

T

R

K

-

K

-

V

-

C

-

G

-

V

-

N

-

V

-

I

-

D

G

W

I

R

K

E

P

M

R

R

K

V

D

R

R

A

A

A

D

M

E

M

L

L

L

L

A

R

R

V

V

G

G

L

L

K

K

I

G

D

L

L

G

D

G

E

R

K

R

V

V

V

A

N

Q

L

L

S

S

I

G

S

G

H

Q

K

R

Q

Q

M

R

L

V

E

N

I

I

A

A

K

R

T

A

L

L

M

M

L

G

D

N

A

P

K

Q

V

L

L

L

I

L

M

A

D

D

E
|
E

P

P

T

T

S

S

S

A

L

L

T

D

N

A

K

R

E

L

V

S

D

K

Y

E

L

I

F

V

L

E

I

L

M

L

N

R

Q

D

L

V

R

T

K

K

E

E

-

F

G

A

T

L

A

A

I

T

V

L

Y

M

I

V

S

T

H

H

K

D

L

R

A

S

E

Q

I

L

R

-

R

A

I

Y

C

A

D

D

R

R

Y

F

T

V

V

E

M

M

K

A

D

D

G

G

S

K

S

A

V

L

binding phosphoaminophosphonic acid-adenylate ester: S43 (≠ N40), G44 (= G41), G46 (= G43), K47 (= K44), S48 (= S45), T49 (= T46), Q88 (= Q87), E163 (= E171)
binding magnesium ion: S48 (= S45), Q88 (= Q87)

Sites not aligning to the query:

binding phosphoaminophosphonic acid-adenylate ester: 14

3c4jA Abc protein artp in complex with atp-gamma-s
30% identity, 44% coverage: 6:227/510 of query aligns to 4:220/242 of 3c4jA

query
sites
3c4jA

I

I

S

D

M

V

A

H

G

Q

I

L

G

K

K

K

S

S

F
|
F

G

G

P

S

V

L

H

E

A
x
V

L

L

K

K

S

G

V

I

D

N

L

V

T

H

I

I

Y

R

P

E

H

G

E

E

I

V

H

V

A

V

L

V

L

I

G

G

E

P

N
x
S

G
|
G

A
x
S

G
|
G

K
|
K

S
|
S

T
|
T

L

F

M

L

K

R

V

C

L

L

S

N

G

L

I

L

H

E

E

D

P

F

T

D

K

E

G

G

T

E

I

I

T

I

I

I

N

D

N

G

V

I

N

N

Y

L

D

K

K

A

L

K

D

D

H

T

K

N

L

L

-

N

A

K

A

V

Q

R

L

E

G

E

I

V

G

G

I

M

I

V

Y

F

Q

Q

E

R

L

F

S

N

V

L

I

F

D

P

E

H

L

M

T

T

V

V

L

L

E

N

N

N

L

I

Y

T

I

L

G

A

R

P

H

M

L

K

T

V

K

R

K

K

V

-

C

-

G

-

V

-

N

-

V

-

I

-

D

-

W

W

-

P

R

R

E

E

M

K

R

A

V

E

R

A

A

K

A

A

M

M

M

E

L

L

L

L

-

D

R

K

V

V

G

G

L

L

K

K

I

D

D

K

L

A

D

H

E

A

K

Y

V

P

V

D

N

S

L

L

S

S

I

G

S

G

H

Q

K

A

Q

Q

M

R

L

V

E

A

I

I

A

A

K

R

T

A

L

L

M

A

L

M

D

E

A

P

K

K

V

I

L

M

I

L

M

F

D

D

E

E

P

P

T

T

S

S

S

A

L

L

T

D

N

P

K

E

E

M

V

V

D

G

Y

E

L

V

F

L

L

S

I

V

M

M

N

K

Q

Q

L

L

R

A

K

N

E

E

G

G

T

M

A

T

I

M

V

V

Y

V

I

V

S

T

H

H

K

E

L

M

A

G

E

F

I

A

R

R

R

E

I

V

C

G

D

D

R

R

Y

V

T

L

V

F

M

M

K

D

D

G

G

G

S

Y

S

I

V

I

C

E

S

E

G

G

binding phosphothiophosphoric acid-adenylate ester: F13 (= F15), V18 (≠ A20), S38 (≠ N40), G39 (= G41), S40 (≠ A42), G41 (= G43), K42 (= K44), S43 (= S45), T44 (= T46)

3c41J Abc protein artp in complex with amp-pnp/mg2+
30% identity, 44% coverage: 6:227/510 of query aligns to 4:220/242 of 3c41J

query
sites
3c41J

I

I

S

D

M

V

A

H

G

Q

I

L

G

K

K

K

S

S

F
|
F

G

G

P

S

V

L

H

E

A
x
V

L

L

K

K

S

G

V

I

D

N

L

V

T

H

I

I

Y

R

P

E

H

G

E

E

I

V

H

V

A

V

L

V

L

I

G

G

E

P

N
x
S

G
|
G

A

S

G
|
G

K
|
K

S
|
S

T
|
T

L

F

M

L

K

R

V

C

L

L

S

N

G

L

I

L

H

E

E

D

P

F

T

D

K

E

G

G

T

E

I

I

T

I

I

I

N

D

N

G

V

I

N

N

Y

L

D

K

K

A

L

K

D

D

H

T

K

N

L

L

-

N

A

K

A

V

Q

R

L

E

G

E

I

V

G

G

I

M

I

V

Y

F

Q

Q

E

R

L

F

S

N

V

L

I

F

D

P

E

H

L

M

T

T

V

V

L

L

E

N

N

N

L

I

Y

T

I

L

G

A

R

P

H

M

L

K

T

V

K

R

K

K

V

-

C

-

G

-

V

-

N

-

V

-

I

-

D

-

W

W

-

P

R

R

E

E

M

K

R

A

V

E

R

A

A

K

A

A

M

M

M

E

L

L

L

L

-

D

R

K

V

V

G

G

L

L

K

K

I

D

D

K

L

A

D

H

E

A

K

Y

V

P

V

D

N

S

L

L

S

S

I

G

S

G

H

Q

K

A

Q

Q

M

R

L

V

E

A

I

I

A

A

K

R

T

A

L

L

M

A

L

M

D

E

A

P

K

K

V

I

L

M

I

L

M

F

D
|
D

E

E

P

P

T

T

S

S

S

A

L

L

T

D

N

P

K

E

E

M

V

V

D

G

Y

E

L

V

F

L

L

S

I

V

M

M

N

K

Q

Q

L

L

R

A

K

N

E

E

G

G

T

M

A

T

I

M

V

V

Y

V

I

V

S

T

H

H

K

E

L

M

A

G

E

F

I

A

R

R

R

E

I

V

C

G

D

D

R

R

Y

V

T

L

V

F

M

M

K

D

D

G

G

G

S

Y

S

I

V

I

C

E

S

E

G

G

binding phosphoaminophosphonic acid-adenylate ester: F13 (= F15), V18 (≠ A20), S38 (≠ N40), G39 (= G41), G41 (= G43), K42 (= K44), S43 (= S45), T44 (= T46)
binding magnesium ion: S43 (= S45), D163 (= D170)

2olkA Abc protein artp in complex with adp-beta-s
30% identity, 44% coverage: 6:227/510 of query aligns to 4:220/242 of 2olkA

query
sites
2olkA

I

I

S

D

M

V

A

H

G

Q

I

L

G

K

K

K

S

S

F
|
F

G

G

P

S

V

L

H

E

A
x
V

L

L

K

K

S

G

V

I

D

N

L

V

T

H

I

I

Y

R

P

E

H

G

E

E

I

V

H

V

A

V

L

V

L

I

G

G

E

P

N
x
S

G
|
G

A
x
S

G
|
G

K
|
K

S
|
S

T
|
T

L

F

M

L

K

R

V

C

L

L

S

N

G

L

I

L

H

E

E

D

P

F

T

D

K

E

G

G

T

E

I

I

T

I

I

I

N

D

N

G

V

I

N

N

Y

L

D

K

K

A

L

K

D

D

H

T

K

N

L

L

-

N

A

K

A

V

Q

R

L

E

G

E

I

V

G

G

I

M

I

V

Y

F

Q

Q

E

R

L

F

S

N

V

L

I

F

D

P

E

H

L

M

T

T

V

V

L

L

E

N

N

N

L

I

Y

T

I

L

G

A

R

P

H

M

L

K

T

V

K

R

K

K

V

-

C

-

G

-

V

-

N

-

V

-

I

-

D

-

W

W

-

P

R

R

E

E

M

K

R

A

V

E

R

A

A

K

A

A

M

M

M

E

L

L

L

L

-

D

R

K

V

V

G

G

L

L

K

K

I

D

D

K

L

A

D

H

E

A

K

Y

V

P

V

D

N

S

L

L

S

S

I

G

S

G

H

Q

K

A

Q

Q

M

R

L

V

E

A

I

I

A

A

K

R

T

A

L

L

M

A

L

M

D

E

A

P

K

K

V

I

L

M

I

L

M

F

D

D

E

E

P

P

T

T

S

S

S

A

L

L

T

D

N

P

K

E

E

M

V

V

D

G

Y

E

L

V

F

L

L

S

I

V

M

M

N

K

Q

Q

L

L

R

A

K

N

E

E

G

G

T

M

A

T

I

M

V

V

Y

V

I

V

S

T

H

H

K

E

L

M

A

G

E

F

I

A

R

R

R

E

I

V

C

G

D

D

R

R

Y

V

T

L

V

F

M

M

K

D

D

G

G

G

S

Y

S

I

V

I

C

E

S

E

G

G

binding 5'-o-[(r)-hydroxy(thiophosphonooxy)phosphoryl]adenosine: F13 (= F15), V18 (≠ A20), S38 (≠ N40), G39 (= G41), S40 (≠ A42), G41 (= G43), K42 (= K44), S43 (= S45), T44 (= T46)

2oljA Abc protein artp in complex with adp/mg2+
30% identity, 44% coverage: 6:227/510 of query aligns to 4:220/242 of 2oljA

query
sites
2oljA

I

I

S

D

M

V

A

H

G

Q

I

L

G

K

K

K

S

S

F
|
F

G

G

P

S

V

L

H

E

A
x
V

L

L

K

K

S

G

V

I

D

N

L

V

T

H

I

I

Y

R

P

E

H

G

E

E

I

V

H

V

A

V

L

V

L

I

G

G

E

P

N
x
S

G
|
G

A
x
S

G
|
G

K
|
K

S
|
S

T
|
T

L

F

M

L

K

R

V

C

L

L

S

N

G

L

I

L

H

E

E

D

P

F

T

D

K

E

G

G

T

E

I

I

T

I

I

I

N

D

N

G

V

I

N

N

Y

L

D

K

K

A

L

K

D

D

H

T

K

N

L

L

-

N

A

K

A

V

Q

R

L

E

G

E

I

V

G

G

I

M

I

V

Y

F

Q

Q

E

R

L

F

S

N

V

L

I

F

D

P

E

H

L

M

T

T

V

V

L

L

E

N

N

N

L

I

Y

T

I

L

G

A

R

P

H

M

L

K

T

V

K

R

K

K

V

-

C

-

G

-

V

-

N

-

V

-

I

-

D

-

W

W

-

P

R

R

E

E

M

K

R

A

V

E

R

A

A

K

A

A

M

M

M

E

L

L

L

L

-

D

R

K

V

V

G

G

L

L

K

K

I

D

D

K

L

A

D

H

E

A

K

Y

V

P

V

D

N

S

L

L

S

S

I

G

S

G

H

Q

K

A

Q

Q

M

R

L

V

E

A

I

I

A

A

K

R

T

A

L

L

M

A

L

M

D

E

A

P

K

K

V

I

L

M

I

L

M

F

D

D

E

E

P

P

T

T

S

S

S

A

L

L

T

D

N

P

K

E

E

M

V

V

D

G

Y

E

L

V

F

L

L

S

I

V

M

M

N

K

Q

Q

L

L

R

A

K

N

E

E

G

G

T

M

A

T

I

M

V

V

Y

V

I

V

S

T

H

H

K

E

L

M

A

G

E

F

I

A

R

R

R

E

I

V

C

G

D

D

R

R

Y

V

T

L

V

F

M

M

K

D

D

G

G

G

S

Y

S

I

V

I

C

E

S

E

G

G

binding adenosine-5'-diphosphate: F13 (= F15), V18 (≠ A20), S38 (≠ N40), G39 (= G41), S40 (≠ A42), G41 (= G43), K42 (= K44), S43 (= S45), T44 (= T46)

1ji0A Crystal structure analysis of the abc transporter from thermotoga maritima
29% identity, 44% coverage: 15:239/510 of query aligns to 16:233/240 of 1ji0A

query
sites
1ji0A

F
x
Y

G

G

P

A

V

I

H

H

A

A

L

I

K

K

S

G

V

I

D

D

L

L

T

K

I

V

Y

P

P

R

H

G

E

Q

I

I

H

V

A

T

L

L

L

I

G

G

E

A

N

N

G
|
G

A

A

G
|
G

K
|
K

S
x
T

T
|
T

L

T

M

L

K

S

V

A

L

I

S

A

G

G

I

L

H

V

E

R

P

A

T

Q

K

K

G

G

T

K

I

I

T

I

I

F

N

N

N

G

V

Q

N

D

Y

I

D

T

K

N

L

K

D

P

H

A

K

H

L

V

A

I

A

N

Q

R

L

M

G

G

I

I

G

A

I

L

I

V

Y

P

Q
x
E

E

G

L

R

S

R

V

I

I

F

D

P

E

E

L

L

T

T

V

V

L

Y

E

E

N

N

L

L

Y

M

I

M

G

G

R

A

H

Y

L

N

T

R

K

K

K

D

V

K

C

E

G

G

V

I

N

K

V

R

-

D

I

L

D

E

W

W

R

-

E

-

M

-

R

-

V

-

R

-

A

-

A

-

M

I

M

F

L

S

L

L

R

F

V

P

G

R

L

L

K

K

I

E

D

R

L

L

D

K

E

Q

K

L

V

G

V

G

N

T

L

L

S

S

I

G

S

G

H

E

K

Q

Q

Q

M

M

L

L

E

A

I

I

A

G

K

R

T

A

L

L

M

M

L

S

D

R

A

P

K

K

V

L

L

L

I

M

M

M

D

D

E
|
E

P

P

T

S

S

L

S

G

L

L

T

A

N

P

K

I

E

L

V

V

D

S

Y

E

L

V

F

F

L

E

I

V

M

I

N

Q

Q

K

L

I

R

N

K

Q

E

E

G

G

T

T

A

T

I

I

V

L

Y

L

I

V

S

E

H

Q

K

N

L

A

A

L

E

G

I

A

R

L

R

K

I

V

C

A

D

H

R

Y

Y

G

T

Y

V

V

M

L

K

E

D

T

G

G

S

Q

S

I

V

V

C

L

S

E

G

G

M

K

V

A

S

S

E

E

V

L

N

L

N

D

D

N

D

E

I

M

V

V

R

R

binding adenosine-5'-triphosphate: Y16 (≠ F15), G42 (= G41), G44 (= G43), K45 (= K44), T46 (≠ S45), T47 (= T46), E88 (≠ Q87), E165 (= E171)

7arlD Lolcde in complex with lipoprotein and adp (see paper)
31% identity, 39% coverage: 21:221/510 of query aligns to 22:218/222 of 7arlD

query
sites
7arlD

L

L

K

H

S

N

V

V

D

S

L

F

T

S

I

V

Y

G

P

E

H

G

E

E

I

M

H

M

A

A

L

I

L

V

G

G

E

S

N

S

G
|
G

A

S

G
|
G

K
|
K

S
|
S

T

T

L

L

M

L

K

H

V

L

L

L

S

G

G

G

I

L

H

D

E

T

P

P

T

T

K

S

G

G

T

D

I

V

T

I

I

F

N

N

N

G

V

Q

N

P

Y

M

D

S

K

K

L

L

D

S

H

S

K

A

L

A

A

K

A

A

Q

E

L

L

-

R

-

N

-

Q

G

K

I

L

G

G

I

F

I

I

Y

Y

Q

Q

E

F

L

H

S

H

V

L

I

L

D

P

E

D

L

F

T

T

V

A

L

L

E

E

N

N

L

V

Y

A

I

M

G

P

R

L

H

L

L

I

T

G

K

K

K

K

V

K

C

P

G

A

V

-

N

-

V

-

I

-

D

-

W

-

R

-

E

E

M

I

R

N

V

S

R

R

A

A

A

L

M

E

M

M

L

L

L

K

R

A

V

V

G

G

L

L

K

D

I

H

D

R

L

A

D

N

E

H

K

R

V

P

V

S

N

E

L

L

S

S

I

G

S

G

H

E

K

R

Q

Q

M

R

L

V

E

A

I

I

A

A

K

R

T

A

L

L

M

V

L

N

D

N

A

P

K

R

V

L

L

V

I

L

M

A

D

D

E

E

P

P

T

T

S

G

S

N

L

L

T

D

N

A

K

R

E

N

V

A

D

D

Y

S

L

I

F

F

L

Q

I

L

M

L

N

G

Q

E

L

L

-

N

R

R

K

L

E

Q

G

G

T

T

A

A

I

F

V

L

Y

V

I

V

S

T

H

H

K

D

L

L

A

Q

E

L

I

A

R

K

R

R

I

M

C

S

D

-

R

R

Y

Q

T

L

V

E

M

M

K

R

D

D

G

G

binding adenosine-5'-diphosphate: G42 (= G41), G44 (= G43), K45 (= K44), S46 (= S45)

P75957 Lipoprotein-releasing system ATP-binding protein LolD; EC 7.6.2.- from Escherichia coli (strain K12) (see paper)
31% identity, 39% coverage: 21:221/510 of query aligns to 25:221/233 of P75957

query
sites
P75957

L

L

K

H

S

N

V

V

D

S

L

F

T

S

I

V

Y

G

P

E

H

G

E

E

I

M

H

M

A

A

L

I

L

V

G
|
G

E

S

N

S

G

G

A

S

G

G

K

K

S

S

T

T

L

L

M

L

K

H

V

L

L

L

S

G

G

G

I

L

H

D

E

T

P

P

T

T

K

S

G

G

T

D

I

V

T

I

I

F

N

N

N

G

V

Q

N

P

Y

M

D

S

K

K

L

L

D

S

H

S

K

A

L

A

A

K

A

A

Q

E

L

L

-

R

-

N

-

Q

G

K

I

L

G

G

I

F

I

I

Y

Y

Q

Q

E

F

L

H

S

H

V

L

I

L

D

P

E

D

L

F

T

T

V

A

L

L

E

E

N

N

L

V

Y

A

I

M

G

P

R

L

H

L

L

I

T

G

K

K

K

K

V

K

C

P

G

A

V

-

N

-

V

-

I

-

D

-

W

-

R

-

E

E

M

I

R

N

V

S

R

R

A

A

A

L

M

E

M

M

L

L

L

K

R

A

V

V

G

G

L

L

K

D

I

H

D

R

L

A

D

N

E

H

K

R

V

P

V

S

N

E

L

L

S

S

I

G

S

G

H

E

K

R

Q

Q

M

R

L

V

E

A

I

I

A

A

K

R

T

A

L

L

M

V

L

N

D

N

A

P

K

R

V

L

L

V

I

L

M

A

D

D

E

E

P

P

T

T

S

G

S

N

L

L

T

D

N

A

K

R

E

N

V

A

D

D

Y

S

L

I

F

F

L

Q

I

L

M

L

N

G

Q

E

L

L

-

N

R

R

K

L

E

Q

G

G

T

T

A

A

I

F

V

L

Y

V

I

V

S

T

H

H

K

D

L

L

A

Q

E

L

I

A

R

K

R

R

I

M

C

S

D

-

R

R

Y

Q

T

L

V

E

M

M

K

R

D

D

G

G

G42 (= G38) mutation to D: Loss of lipoprotein release when overexpressed.

7mdyC Lolcde nucleotide-bound
31% identity, 39% coverage: 21:221/510 of query aligns to 22:218/226 of 7mdyC

query
sites
7mdyC

L

L

K

H

S

N

V

V

D

S

L

F

T

S

I

V

Y

G

P

E

H

G

E

E

I

M

H

M

A

A

L

I

L

V

G

G

E

S

N

S

G
|
G

A
x
S

G
|
G

K
|
K

S
|
S

T
|
T

L

L

M

L

K

H

V

L

L

L

S

G

G

G

I

L

H

D

E

T

P

P

T

T

K

S

G

G

T

D

I

V

T

I

I

F

N

N

N

G

V

Q

N

P

Y

M

D

S

K

K

L

L

D

S

H

S

K

A

L

A

A

K

A

A

Q

E

L

L

-

R

-

N

-

Q

G

K

I

L

G

G

I

F

I

I

Y

Y

Q
|
Q

E

F

L

H

S

H

V

L

I

L

D

P

E

D

L

F

T

T

V

A

L

L

E

E

N

N

L

V

Y

A

I

M

G

P

R

L

H

L

L

I

T

G

K

K

K

K

V

K

C

P

G

A

V

-

N

-

V

-

I

-

D

-

W

-

R

-

E

E

M

I

R

N

V

S

R

R

A

A

A

L

M

E

M

M

L

L

L

K

R

A

V

V

G

G

L

L

K

D

I

H

D

R

L

A

D

N

E
x
H

K

R

V

P

V

S

N
x
E

L

L

S
|
S

I
x
G

S
x
G

H

E

K

R

Q

Q

M

R

L

V

E

A

I

I

A

A

K

R

T

A

L

L

M

V

L

N

D

N

A

P

K

R

V

L

L

V

I

L

M

A

D

D

E
|
E

P

P

T

T

S

G

S
x
N

L

L

T

D

N

A

K

R

E

N

V

A

D

D

Y

S

L

I

F

F

L

Q

I

L

M

L

N

G

Q

E

L

L

-

N

R

R

K

L

E

Q

G

G

T

T

A

A

I

F

V

L

Y

V

I

V

S

T

H
|
H

K

D

L

L

A

Q

E

L

I

A

R

K

R

R

I

M

C

S

D

-

R

R

Y

Q

T

L

V

E

M

M

K

R

D

D

G

G

binding adp orthovanadate: G42 (= G41), S43 (≠ A42), G44 (= G43), K45 (= K44), S46 (= S45), T47 (= T46), Q91 (= Q87), H138 (≠ E141), E142 (≠ N145), S144 (= S147), G145 (≠ I148), G146 (≠ S149), E168 (= E171), N172 (≠ S175), H201 (= H203)
binding magnesium ion: S46 (= S45), Q91 (= Q87)

Sites not aligning to the query:

binding adp orthovanadate: 12

7v8iD Lolcd(e171q)e with bound amppnp in nanodiscs (see paper)
31% identity, 39% coverage: 21:221/510 of query aligns to 24:220/229 of 7v8iD

query
sites
7v8iD

L

L

K

H

S

N

V

V

D

S

L

F

T

S

I

V

Y

G

P

E

H

G

E

E

I

M

H

M

A

A

L

I

L

V

G

G

E

S

N
x
S

G
|
G

A

S

G
|
G

K
|
K

S
|
S

T
|
T

L

L

M

L

K

H

V

L

L

L

S

G

G

G

I

L

H

D

E

T

P

P

T

T

K

S

G

G

T

D

I

V

T

I

I

F

N

N

N

G

V

Q

N

P

Y

M

D

S

K

K

L

L

D

S

H

S

K

A

L

A

A

K

A

A

Q

E

L

L

-

R

-

N

-

Q

G

K

I

L

G

G

I

F

I

I

Y

Y

Q
|
Q

E

F

L

H

S

H

V

L

I

L

D

P

E

D

L

F

T

T

V

A

L

L

E

E

N

N

L

V

Y

A

I

M

G

P

R

L

H

L

L

I

T

G

K

K

K

K

V

K

C

P

G

A

V

-

N

-

V

-

I

-

D

-

W

-

R

-

E

E

M

I

R

N

V

S

R

R

A

A

A

L

M

E

M

M

L

L

L

K

R

A

V

V

G

G

L

L

K

D

I

H

D
x
R

L

A

D

N

E
x
H

K

R

V

P

V

S

N
x
E

L

L

S
|
S

I

G

S
x
G

H
x
E

K

R

Q

Q

M

R

L

V

E

A

I

I

A

A

K

R

T

A

L

L

M

V

L

N

D

N

A

P

K

R

V

L

L

V

I

L

M

A

D

D

E

Q

P

P

T

T

S

G

S

N

L

L

T

D

N

A

K

R

E

N

V

A

D

D

Y

S

L

I

F

F

L

Q

I

L

M

L

N

G

Q

E

L

L

-

N

R

R

K

L

E

Q

G

G

T

T

A

A

I

F

V

L

Y

V

I

V

S

T

H
|
H

K

D

L

L

A

Q

E

L

I

A

R

K

R

R

I

M

C

S

D

-

R

R

Y

Q

T

L

V

E

M

M

K

R

D

D

G

G

binding phosphoaminophosphonic acid-adenylate ester: S43 (≠ N40), G44 (= G41), G46 (= G43), K47 (= K44), S48 (= S45), T49 (= T46), Q93 (= Q87), R137 (≠ D138), H140 (≠ E141), E144 (≠ N145), S146 (= S147), G148 (≠ S149), E149 (≠ H150), H203 (= H203)
binding magnesium ion: S48 (= S45), Q93 (= Q87)

Sites not aligning to the query:

binding phosphoaminophosphonic acid-adenylate ester: 23

4yerA Crystal structure of an abc transporter atp-binding protein (tm_1403) from thermotoga maritima msb8 at 2.35 a resolution
29% identity, 46% coverage: 13:246/510 of query aligns to 12:231/285 of 4yerA

query
sites
4yerA

K

K

S

K

F
|
F

G

G

P

D

V
x
F

H

E

A

A

L

V

K

K

S

G

V

V

D

S

L

F

T

S

I

V

Y

K

P

K

H

G

E

E

I

I

H

F

A

A

L

F

L

L

G

G

E

P

N
|
N

G
|
G

A

A

G
|
G

K
|
K

S
x
T

T
|
T

L

T

M

I

K

H

V

M

L

L

S

T

G

T

I

L

H

L

E

K

P

P

T

T

K

S

G

G

T

K

I

A

T

W

I

V

N

-

N

-

V

A

N

G

Y

H

D

D

K

V

L

L

D

K

H

E

K

P

L

R

A

E

A

V

Q

R

L

R

G

K

I

I

G

G

I

I

I

V

Y

F

Q

Q

E

D

L

Q

S

S

V

L

I

D

D

R

E

E

L

L

T

T

V

A

L

Y

E

E

N

N

L

M

Y

Y

I

I

G

-

R

-

H

-

L

-

T

H

K

G

K

K

V

I

C

Y

G

G

V

Y

N

G

V

-

I

-

D

-

W

G

R

E

E

K

M

L

R

K

V

K

R

R

A

I

A

L

M

E

M

L

L

L

L

E

R

F

V

V

G

E

L

L

K

L

I

E

D

F

L

K

D

D

E

K

K

P

V

V

V

K

N
x
T

L
x
F

S
|
S

I

G

S

G

H

M

K

A

Q

R

M

R

L

L

E

E

I

I

A

A

K

R

T

S

L

L

M

I

L

H

D

E

A

P

K

E

V

V

L

L

I

F

M

L

D

D

E

E

P

P

T

T

S

I

S

G

L

L

T

D

N

P

K

H

E

T

V

R

D

A

Y

H

L

M

F

W

L

E

I

Y

M

I

N

S

Q

K

L

M

R

K

K

K

E

E

G

H

T

N

A

M

I

T

V

I

Y

F

I

L

-

T

S

T

H

H

K

Y

L

M

A

D

E

E

I

A

R

E

R

Q

I

L

C

A

D

D

R

R

Y

V

T

A

V

I

M

I

K

D

D

H

G

G

S

K

S

I

V

I

C

A

S

L

G

G

M

T

V

P

S

T

E

E

V

L

N

K

N

R

D

-

D

-

I

-

V

-

R

-

L

-

M

M

V

V

G

G

R

K

E

E

L

I

binding adenosine-5'-diphosphate: F14 (= F15), F17 (≠ V18), N39 (= N40), G40 (= G41), G42 (= G43), K43 (= K44), T44 (≠ S45), T45 (= T46), T135 (≠ N145), F136 (≠ L146), S137 (= S147)

P75831 Macrolide export ATP-binding/permease protein MacB; EC 7.6.2.- from Escherichia coli (strain K12) (see paper)
29% identity, 44% coverage: 2:224/510 of query aligns to 1:222/648 of P75831

query
sites
P75831

L

M

T

T

P

P

Y

L

I

L

S

E

M

L

A

K

G

D

I

I

G

R

K

R

S

S

F

Y

G

P

P

A

-

G

-

D

-

E

-

Q

V

V

H

E

A

V

L

L

K

K

S

G

V

I

D

S

L

L

T

D

I

I

Y

Y

P

A

H

G

E

E

I

M

H

V

A

A

L

I

L

V

G

G

E

A

N

S

G

G

A

S

G

G

K
|
K

S

S

T

T

L

L

M

M

K

N

V

I

L

L

S

G

G

C

I

L

H

D

E

K

P

A

T

T

K

S

G

G

T

T

I

Y

T

R

I

V

N

A

N

G

V

Q

N

D

Y

V

D

A

K

T

L

L

D

D

H

A

K

D

L

A

A

L

A

A

Q

Q

L

L

-

R

-

R

-

E

G

H

I

F

G

G

I

F

I

I

Y

F

Q

Q

E

R

L

Y

S

H

V

L

I

L

D

S

E

H

L

L

T

T

V

A

L

E

E

Q

N

N

L

V

Y

-

I

-

G

-

R

-

H

-

L

-

T

-

K

-

K

E

V

V

C

P

G

A

V

V

N

Y

V

A

I

G

D

L

W

E

R

R

E

K

M

Q

R

R

V

L

-

L

R

R

A

A

A

Q

M

E

M

L

L

L

L

Q

R

R

V

L

G

G

L

L

K

E

I

D

D

R

L

T

D

E

E

Y

K

Y

V

P

V

A

N

Q

L

L

S

S

I

G

S

G

H

Q

K

Q

Q

Q

M

R

L

V

E

S

I

I

A

A

K

R

T

A

L

L

M

M

L

N

D

G

A

G

K

Q

V

V

L

I

I

L

M

A

D
|
D

E

E

P

P

T

T

S

G

S

A

L

L

T

D

N

S

K

H

E

S

V

G

D

E

Y

E

L

V

F

M

L

A

I

I

M

L

N

H

Q

Q

L

L

R

R

K

D

E

R

G

G

T

H

A

T

I

V

V

I

Y

I

I

V

S

T

H

H

K

D

L

-

A

P

E

Q

I

V

R

A

R

A

I

Q

C

A

D

E

R

R

Y

V

T

I

V

E

M

I

K

R

D

D

G

G

S

E

S

I

V

V

K47 (= K44) mutation to L: Lack of activity.
D169 (= D170) mutation to N: Lack of activity.

5ws4A Crystal structure of tripartite-type abc transporter macb from acinetobacter baumannii (see paper)
29% identity, 40% coverage: 18:221/510 of query aligns to 21:219/650 of 5ws4A

query
sites
5ws4A

V

I

H

Q

A

I

L

L

K

K

S

G

V

I

D

D

L

L

T

T

I

I

Y

Y

P

E

H

G

E

E

I

L

H

V

A

A

L

I

L

V

G

G

E

Q

N
x
S

G
|
G

A

S

G

G

K

K

S
|
S

T

T

L

L

M

M

K

N

V

I

L

L

S

G

G

C

I

L

H

D

E

R

P

P

T

T

K

S

G

G

T

S

I

Y

T

K

I

V

N

N

N

G

V

Q

N

E

Y

T

D

G

K

K

L

L

D

E

H

P

K

D

L

Q

A

L

A

A

Q

Q

L

L

G

R

-

R

-

E

-

Y

I

F

G

G

I

F

I

I

Y

F

Q

Q

E

R

L

Y

S

H

V

L

I

L

D

G

E

D

L

L

T

S

V

A

L

E

E

G

N

N

L

V

Y

-

I

-

G

-

R

-

H

E

L

V

T

P

K

A

K

V

V

Y

C

A

G

G

V

V

N

T

V

P

I

A

D

D

W

R

R

K

E

Q

M

-

R

-

V

-

R

R

A

A

A

T

M

A

M

L

L

L

L

T

R

E

V

L

G

G

L

L

K

G

I

T

D

K

L

T

D

Q

E

N

K

R

V

P

V

S

N

Q

L

L

S

S

I

G

S

G

H

Q

K

Q

Q

Q

M

R

L

V

E

S

I

I

A

A

K

R

T

A

L

L

M

M

L

N

D

G

A

G

K

D

V

V

L

I

I

L

M

A

D

D

E

E

P

P

T

T

S

G

S

A

L

L

T

D

N

S

K

H

E

S

V

G

D

V

Y

E

L

V

F

M

L

R

I

I

M

L

N

R

Q

E

L

L

R

N

K

A

E

A

G

G

T

H

A

T

I

I

V

I

Y

L

I

V

S

T

H

H

K

D

L

M

A

-

E

Q

I

V

R

A

R

K

I

N

C

A

D

T

R

R

Y

I

T

I

V

E

M

I

K

S

D

D

G

G

binding 5'-o-[(r)-hydroxy(thiophosphonooxy)phosphoryl]adenosine: S43 (≠ N40), G44 (= G41), S48 (= S45)

Q8N139 ATP-binding cassette sub-family A member 6; EC 7.6.2.- from Homo sapiens (Human) (see 2 papers)
31% identity, 42% coverage: 12:227/510 of query aligns to 489:695/1617 of Q8N139

query
sites
Q8N139

G

G

K

K

S

S

F

-

G

G

P

K

V

V

H

E

A

A

L

L

K

K

S

G

V

L

D

L

L

F

T

D

I

I

Y

Y

P

E

H

G

E

Q

I

I

H

T

A

A

L

I

L

L

G

G

E

H

N

S

G

G

A

A

G

G

K

K

S

S

T

S

L

L

M

L

K

N

V

I

L

L

S

N

G

G

I

L

H

S

E

V

P

P

T

T

K

E

G

G

T

S

I

V

T

T

I

I

N

Y

N

N

V

K

N

N

Y

L

D

S

K

E

L

M

D

Q

H

D

K

L

L

E

A

E

A

I

Q

R

L

K

G

I

I

T

G

G

I

V

I

C

Y

P

Q

Q

E

F

L

N

S

V

V

Q

I

F

D

D

E

I

L

L

T

T

V

V

L

K

E

E

N

N

L

L

Y

-

I

-

G

-

R

-

H

S

L

L

T

F

K

A

K

K

V

I

C

K

G

G

V

I

N

H

V

L

I

-

D

-

W

-

R

K

E

E

M

V

R

E

V

Q

R

E

A

V

A

Q

M

R

M

I

L

L

L

L

R

E

V

L

G

D

L

M

K

Q

I

N

D

I

L

Q

D

D

E

N

K

L

V

A

V

K

N

H

L

L

S

S

I

E

S

G

H

Q

K

K

Q

R

M

K

L

L

E

T

I

F

A

G

K

I

T

T

L

I

M

L

L

G

D

D

A

P

K

Q

V

I

L

L

I

L

M

L

D

D

E

E

P

P

T

T

S

T

S

G

L

L

T

D

N

P

K

F

E

S

V

R

D

D

Y

Q

L

V

F

W

L

S

I

L

M

L

N

R

Q

E

L

-

R

R

K

R

E

A

G

D

T

H

A

V

I

I

V

L

Y

F

I

S

S

T

H

Q

K

S

L

M

A

D

E

E

I

A

R

D

R

I

I

L

C

A

D

D

R

R

Y

K

T

V

V

I

M

M

K

S

D

N

G

G

S

R

S

L

V

K

C

C

S

A

G

G

Sites not aligning to the query:

282 V → I: in dbSNP:rs4968839
875 M → I: in dbSNP:rs7212506
940 modified: carbohydrate, N-linked (GlcNAc...) asparagine
1322 N → S: in dbSNP:rs2302134

P30750 Methionine import ATP-binding protein MetN; EC 7.4.2.11 from Escherichia coli (strain K12) (see 3 papers)
29% identity, 45% coverage: 6:232/510 of query aligns to 2:228/343 of P30750

query
sites
P30750

I

I

S

K

M

L

A

S

G

N

I

I

G

T

K

K

S

V

F

F

G

H

-

Q

-

G

-

T

-

R

P

T

V

I

H

Q

A

A

L

L

K

N

S

N

V

V

D

S

L

L

T

H

I

V

Y

P

P

A

H

G

E

Q

I

I

H

Y

A

G

L

V

L

I

G

G

E

A

N
x
S

G
|
G

A
|
A

G
|
G

K
|
K

S
|
S

T
|
T

L

L

M

I

K

R

V

C

L

V

S

N

G

L

I

L

H

E

E

R

P

P

T

T

K

E

G

G

T

S

I

V

T

L

I

V

N

D

N

G

V

Q

N

E

Y

L

D

T

K

T

L

L

D

S

H

E

K

S

-

E

-

L

L

T

A

K

A

A

Q

R

L

R

G

Q

I

I

G

G

I

M

I

I

Y

F

Q

Q

E

H

L

F

S

N

V

L

I

L

D

S

E

S

L

R

T

T

V

V

L

F

E

G

N

N

L

V

Y

A

I

L

G

P

R

L

H

E

L

L

T

D

K

N

K

T

V

-

C

-

G

-

V

-

N

-

V

-

I

-

D

P

W

K

R

D

E

E

M

V

R

K

V

R

R

R

A

V

A

T

M

E

M

L

L

L

L

S

R

L

V

V

G

G

L

L

K

G

I

D

D

K

L

H

D

D

E

S

K

Y

V

P

V

S

N

N

L

L

S

S

I

G

S

G

H

Q

K

K

Q

Q

M

R

L

V

E

A

I

I

A

A

K

R

T

A

L

L

M

A

L

S

D

N

A

P

K

K

V

V

L

L

I

L

M

C

D

D

E
|
E

P

A

T

T

S

S

S

A

L

L

T

D

N

P

K

A

E

T

V

T

D

R

Y

S

L

I

F

L

L

E

I

L

M

L

N

K

Q

D

L

I

-

N

R

R

K

R

E

L

G

G

T

L

A

T

I

I

V

L

Y

L

I

I

S

T

H

H

K

E

L

M

A

D

E

V

I

V

R

K

R

R

I

I

C

C

D

D

R

C

Y

V

T

A

V

V

M

I

K

S

D

N

G

G

S

E

S

L

V

I

C

E

S

Q

G

D

M

T

V

V

S

S

E

E

V

V

40:46 (vs. 40:46, 86% identical) binding
E166 (= E171) mutation to Q: Exhibits little ATPase activity.

Sites not aligning to the query:

278:283 binding
295 N→A: Reduces the binding of L-methionine to undetectable levels.
295:296 binding

3tuzC Inward facing conformations of the metni methionine abc transporter: cy5 semet soak crystal form (see paper)
29% identity, 45% coverage: 6:232/510 of query aligns to 3:229/344 of 3tuzC

query
sites
3tuzC

I

I

S

K

M

L

A

S

G

N

I

I

G

T

K

K

S

V

F
|
F

G

H

-
x
Q

-

G

-

T

-

R

P

T

V

I

H

Q

A

A

L

L

K

N

S

N

V

V

D

S

L

L

T

H

I

V

Y

P

P

A

H

G

E

Q

I

I

H

Y

A

G

L

V

L

I

G

G

E

A

N

S

G
|
G

A

A

G
|
G

K
|
K

S
|
S

T
|
T

L

L

M

I

K

R

V

C

L

V

S

N

G

L

I

L

H

E

E

R

P

P

T

T

K

E

G

G

T

S

I

V

T

L

I

V

N

D

N

G

V

Q

N

E

Y

L

D

T

K

T

L

L

D

S

H

E

K

S

-

E

-

L

L

T

A

K

A

A

Q

R

L

R

G

Q

I

I

G

G

I

M

I

I

Y

F

Q

Q

E

H

L

F

S

N

V

L

I

L

D

S

E

S

L

R

T

T

V

V

L

F

E

G

N

N

L

V

Y

A

I

L

G

P

R

L

H

E

L

L

T

D

K

N

K

T

V

-

C

-

G

-

V

-

N

-

V

-

I

-

D

P

W

K

R

D

E

E

M

V

R

K

V

R

R

R

A

V

A

T

M

E

M

L

L

L

L

S

R

L

V

V

G

G

L

L

K

G

I

D

D

K

L

H

D

D

E

S

K

Y

V

P

V

S

N

N

L

L

S

S

I

G

S

G

H

Q

K

K

Q

Q

M

R

L

V

E

A

I

I

A

A

K

R

T

A

L

L

M

A

L

S

D

N

A

P

K

K

V

V

L

L

I

L

M

C

D

D

E

Q

P

A

T

T

S

S

S

A

L

L

T

D

N

P

K

A

E

T

V

T

D

R

Y

S

L

I

F

L

L

E

I

L

M

L

N

K

Q

D

L

I

-

N

R

R

K

R

E

L

G

G

T

L

A

T

I

I

V

L

Y

L

I

I

S

T

H

H

K

E

L

M

A

D

E

V

I

V

R

K

R

R

I

I

C

C

D

D

R

C

Y

V

T

A

V

V

M

I

K

S

D

N

G

G

S

E

S

L

V

I

C

E

S

Q

G

D

M

T

V

V

S

S

E

E

V

V

binding adenosine-5'-diphosphate: F12 (= F15), Q14 (vs. gap), G42 (= G41), G44 (= G43), K45 (= K44), S46 (= S45), T47 (= T46)

Sites not aligning to the query:

binding selenomethionine: 274, 279, 281, 282, 283, 284, 311, 313

Query Sequence

>BWI76_RS02160 FitnessBrowser__Koxy:BWI76_RS02160
MLTPYISMAGIGKSFGPVHALKSVDLTIYPHEIHALLGENGAGKSTLMKVLSGIHEPTKG
TITINNVNYDKLDHKLAAQLGIGIIYQELSVIDELTVLENLYIGRHLTKKVCGVNVIDWR
EMRVRAAMMLLRVGLKIDLDEKVVNLSISHKQMLEIAKTLMLDAKVLIMDEPTSSLTNKE
VDYLFLIMNQLRKEGTAIVYISHKLAEIRRICDRYTVMKDGSSVCSGMVSEVNNDDIVRL
MVGRELQNRFNAMKESTGNIVRETVFEVKNVTSHDKKKVRDISFSVNRGEILGFAGLVGS
GRTELMDCLFGVDKRSGGEIYLNGKAISPRSPLDALKKGMGYITESRRENGFFPNFSIAQ
NMAVSQSLKRGGYKGAMGLFREGDERKTAEEQRKLLALKCHSVDQNITELSGGNQQKVLI
SKWLCCSPEVIIFDEPTRGIDVGAKAEIYKVMRQLADDGKVILMVSSELPEIIAVCDRIA
VFCEGRLTQILTNRDDLSEEEIMAWALPQE

Or try a new SitesBLAST search

SitesBLAST's Database

SitesBLAST's database includes (1) SwissProt entries with experimentally-supported functional features; and (2) protein structures with bound ligands, from the BioLip database.

by Morgan Price, Arkin group
Lawrence Berkeley National Laboratory