SitesBLAST

SitesBLAST – Find functional sites

SitesBLAST

Comparing BWI76_RS02945 FitnessBrowser__Koxy:BWI76_RS02945 to proteins with known functional sites using BLASTp with E ≤ 0.001.

Or try Sites on a Tree, PaperBLAST, Conserved Domains, or compare to all protein structures

Found 3 hits to proteins with known functional sites (download)

P46318 Lichenan-specific phosphotransferase enzyme IIB component; EIIB-Lic; PTS system lichenan-specific EIIB component; EC 2.7.1.- from Bacillus subtilis (strain 168) (see paper)
44% identity, 95% coverage: 3:99/102 of query aligns to 2:98/102 of P46318

query
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P46318

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S37 (≠ P38) modified: Phosphoserine

P69795 PTS system N,N'-diacetylchitobiose-specific EIIB component; EIIB-Chb; IVcel; N,N'-diacetylchitobiose-specific phosphotransferase enzyme IIB component; EC 2.7.1.196 from Escherichia coli (strain K12) (see 2 papers)
43% identity, 89% coverage: 3:93/102 of query aligns to 5:93/106 of P69795

query
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P69795

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C10 (= C8) active site, Phosphocysteine intermediate; mutation to S: Unable to be phosphorylated by EIIA.

2wy2D Nmr structure of the iiachitobiose-iibchitobiose phosphoryl transition state complex of the n,n'-diacetylchitoboise brance of the e. Coli phosphotransferase system. (see paper)
43% identity, 89% coverage: 3:93/102 of query aligns to 3:91/103 of 2wy2D

query
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2wy2D

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binding phosphite ion: C8 (= C8), S9 (≠ A9), A10 (= A10), G11 (= G11), M12 (= M12), S13 (= S13)

Query Sequence

>BWI76_RS02945 FitnessBrowser__Koxy:BWI76_RS02945
MPRIVLCCAAGMSTSMLVNKMTAEAQQRALALEIYAVPVAEFERELPNADVILLGPQVKY
EAARLTELAAPHGKAVAVIDMADYGMMRGAAVLDKALALLNH

Or try a new SitesBLAST search

SitesBLAST's Database

SitesBLAST's database includes (1) SwissProt entries with experimentally-supported functional features; and (2) protein structures with bound ligands, from the BioLip database.

by Morgan Price, Arkin group
Lawrence Berkeley National Laboratory