SitesBLAST

SitesBLAST – Find functional sites

SitesBLAST

Comparing BWI76_RS02995 FitnessBrowser__Koxy:BWI76_RS02995 to proteins with known functional sites using BLASTp with E ≤ 0.001.

Or try Sites on a Tree, PaperBLAST, Conserved Domains, or compare to all protein structures

Found 20 (the maximum) hits to proteins with known functional sites (download)

P0A786 Aspartate carbamoyltransferase catalytic subunit; Aspartate transcarbamylase; ATCase; EC 2.1.3.2 from Escherichia coli (strain K12) (see 4 papers)
93% identity, 100% coverage: 1:311/311 of query aligns to 1:311/311 of P0A786

query
sites
P0A786

M
|
M

A

A

N

N

P

P

L

L

Y

Y

Q

Q

K

K

H

H

I

I

I

I

S

S

I

I

N

N

D

D

L

L

S

S

R

R

E

D

D

D

L

L

E

N

L

L

V

V

L

L

A

A

T

T

A

A

A

A

K

K

L

L

K

K

A

A

H

N

P

P

Q

Q

P

P

E

E

L

L

L

L

K

K

H

H

K

K

V

V

I

I

A

A

S

S

C

C

F

F

F

F

E

E

A

A

S

S

T

T

R
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R

T
|
T

R

R

L

L

S

S

F

F

E

E

T

T

S

S

M

M

H

H

R

R

L

L

G

G

A

A

S

S

V

V

V

V

G

G

F

F

S

S

D

D

S

S

S

A

N

N

T

T

S

S

L

L

G

G

K

K

K

K

G

G

E

E

T

T

L

L

A

A

D

D

T

T

I

I

S

S

V

V

I

I

S

S

T

T

Y

Y

V

V

D

D

A

A

I

I

V

V

M

M

R
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R

H

H

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P

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Q

E

E

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G

A

A

A

A

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R

L

L

A

A

T

T

E

E

F

F

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S

G

G

G

N

I

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P

V

V

L

L

N

N

A

A

G

G

D

D

G

G

A

S

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N

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H
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H

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P

T

T

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Q

T

T

L

L

L

L

D

D

L

L

F

F

T

T

I

I

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Q

E

E

T

T

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Q

G

G

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R

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L

E

D

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N

H

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V

A

A

M

M

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V

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G

D

D

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L

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A

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L

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A

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K

F

F

N

D

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G

N

N

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R

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F

Y

Y

F

F

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I

A

A

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P

D

D

A

A

L

L

A

A

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M

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P

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Y

Y

I

I

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L

D

D

M

M

L

L

D

D

D

E

K

K

G

G

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I

A

A

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M

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A

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M

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E

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E

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Y

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A

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K

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A

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Q

F

F

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V

L

L

R

R

A

A

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A

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N

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M

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K

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V

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L

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H

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P

L
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L

P
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P

R

R

I

V

D

D

E

E

I

I

T

A

T

T

D

D

V

V

D

D

K

K

T

T

P

P

H

H

A

A

W

W

Y

Y

F

F

Q

Q

Q

Q

A

A

G

G

N

N

G

G

I

I

F

F

A

A

R

R

Q

Q

A

A

L

L

L

L

A

A

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L

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L

L

N

N

S

R

D

D

L

L

A

V

L

L

M1 (= M1) modified: Initiator methionine, Removed
R55 (= R55) binding carbamoyl phosphate
T56 (= T56) binding carbamoyl phosphate
R106 (= R106) binding carbamoyl phosphate
H135 (= H135) binding carbamoyl phosphate
Q138 (= Q138) binding carbamoyl phosphate
L268 (= L268) binding carbamoyl phosphate
P269 (= P269) binding carbamoyl phosphate

2ipoA E. Coli aspartate transcarbamoylase complexed with n-phosphonacetyl-l- asparagine (see paper)
93% identity, 100% coverage: 2:311/311 of query aligns to 1:310/310 of 2ipoA

query
sites
2ipoA

A

A

N

N

P

P

L

L

Y

Y

Q

Q

K

K

H

H

I

I

I

I

S

S

I

I

N

N

D

D

L

L

S

S

R

R

E

D

D

D

L

L

E

N

L

L

V

V

L

L

A

A

T

T

A

A

A

A

K

K

L

L

K

K

A

A

H

N

P

P

Q

Q

P

P

E

E

L

L

L

L

K

K

H

H

K

K

V

V

I

I

A

A

S

S

C

C

F

F

F

F

E

E

A

A

S
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S

T
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T

R
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R

T
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T

R

R

L

L

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S

F

F

E

E

T

T

S

S

M

M

H

H

R

R

L

L

G

G

A

A

S

S

V

V

V

V

G

G

F

F

S

S

D

D

S

S

S

A

N

N

T

T

S

S

L

L

G

G

K

K

K
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K

G

G

E

E

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T

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L

A

A

D

D

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T

I

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A

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I

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V

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H

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P

Q

Q

E

E

G

G

A

A

A

A

R

R

L

L

A

A

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T

E

E

F

F

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G

G

G

N

I

V

P

P

V

V

L

L

N

N

A

A

G

G

D

D

G

G

A

S

N

N

Q

Q

H
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H

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P

T

T

Q
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Q

T

T

L

L

L

L

D

D

L

L

F

F

T

T

I

I

Q

Q

E

E

T

T

Q

Q

G

G

R

R

L

L

E

D

N

N

L

L

N

H

V

V

A

A

M

M

V

V

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G

D

D

L

L

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K

Y

Y

G

G

R
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R

T
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T

V

V

H

H

S

S

L

L

T

T

Q

Q

A

A

L

L

A

A

K

K

F

F

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D

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G

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N

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R

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F

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Y

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F

I

I

A

A

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P

D

D

A

A

L

L

A

A

M

M

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P

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Q

Y

Y

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I

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L

D

D

M

M

L

L

D

D

D

E

K

K

G

G

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I

A

A

W

W

S

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L

H

H

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S

A

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I

I

E

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E

E

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V

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M

E

A

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E

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V

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D

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I

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L

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Y

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M

T
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T

R
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R

V

V

Q

Q

K

K

E

E

R

R

L

L

D

D

P

P

S

S

E

E

Y

Y

A

A

N

N

V

V

K

K

A

A

Q

Q

F

F

V

V

L

L

R

R

A

A

A

S

D

D

L

L

E

H

G

N

A

A

R

K

A

A

N

N

M

M

K

K

V

V

L

L

H

H

P
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P

L
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L

P

P

R

R

I

V

D

D

E

E

I

I

T

A

T

T

D

D

V

V

D

D

K

K

T

T

P

P

H

H

A

A

W

W

Y

Y

F

F

Q

Q

Q

Q

A

A

G

G

N

N

G
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G

I

I

F

F

A

A

R

R

Q

Q

A

A

L

L

L

L

A

A

L

L

V

V

L

L

N

N

S

R

D

D

L

L

A

V

L

L

active site: R54 (= R55), T55 (= T56), K84 (= K85), R105 (= R106), H134 (= H135), Q137 (= Q138), T228 (= T229), P266 (= P267), G292 (= G293)
binding n~2~-(phosphonoacetyl)-l-asparagine: S52 (= S53), T53 (= T54), R54 (= R55), T55 (= T56), R105 (= R106), H134 (= H135), R167 (= R168), T168 (= T169), R229 (= R230), L267 (= L268)

2h3eA Structure of wild-type e. Coli aspartate transcarbamoylase in the presence of n-phosphonacetyl-l-isoasparagine at 2.3a resolution (see paper)
93% identity, 100% coverage: 2:311/311 of query aligns to 1:310/310 of 2h3eA

query
sites
2h3eA

A

A

N

N

P

P

L

L

Y

Y

Q

Q

K

K

H

H

I

I

I

I

S

S

I

I

N

N

D

D

L

L

S

S

R

R

E

D

D

D

L

L

E

N

L

L

V

V

L

L

A

A

T

T

A

A

A

A

K

K

L

L

K

K

A

A

H

N

P

P

Q

Q

P

P

E

E

L

L

L

L

K

K

H

H

K

K

V

V

I

I

A

A

S

S

C

C

F

F

F

F

E

E

A

A

S
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S

T
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T

R
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R

T
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T

R

R

L

L

S

S

F

F

E

E

T

T

S

S

M

M

H

H

R

R

L

L

G

G

A

A

S

S

V

V

V

V

G

G

F

F

S

S

D

D

S

S

S

A

N

N

T

T

S

S

L

L

G

G

K

K

K
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K

G

G

E

E

T

T

L

L

A

A

D

D

T

T

I

I

S

S

V

V

I

I

S

S

T

T

Y

Y

V

V

D

D

A

A

I

I

V

V

M

M

R
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R

H

H

P

P

Q

Q

E

E

G

G

A

A

A

A

R

R

L

L

A

A

T

T

E

E

F

F

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S

G

G

G

N

I

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P

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V

L

L

N

N

A

A

G

G

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D

G

G

A

S

N

N

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H
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H

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P

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T

Q
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Q

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T

L

L

L

L

D

D

L

L

F

F

T

T

I

I

Q

Q

E

E

T

T

Q

Q

G

G

R

R

L

L

E

D

N

N

L

L

N

H

V

V

A

A

M

M

V

V

G

G

D

D

L

L

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K

Y

Y

G

G

R
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R

T

T

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V

H

H

S

S

L

L

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T

Q

Q

A

A

L

L

A

A

K

K

F

F

N

D

G

G

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R

F

F

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Y

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F

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I

A

A

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P

D

D

A

A

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L

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A

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M

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P

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Q

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Y

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I

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L

D

D

M

M

L

L

D

D

D

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K

K

G

G

I

I

A

A

W

W

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S

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L

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H

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S

A

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I

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E

E

E

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V

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M

E

A

E

E

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D

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I

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L

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Y

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M

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T

R
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R

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V

Q

Q

K

K

E

E

R

R

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L

D

D

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P

S

S

E

E

Y

Y

A

A

N

N

V

V

K

K

A

A

Q

Q

F

F

V

V

L

L

R

R

A

A

A

S

D

D

L

L

E

H

G

N

A

A

R

K

A

A

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N

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M

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K

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L

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H

P
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P

L
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L

P

P

R

R

I

V

D

D

E

E

I

I

T

A

T

T

D

D

V

V

D

D

K

K

T

T

P

P

H

H

A

A

W

W

Y

Y

F

F

Q

Q

Q

Q

A

A

G

G

N

N

G
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G

I

I

F

F

A

A

R

R

Q

Q

A

A

L

L

L

L

A

A

L

L

V

V

L

L

N

N

S

R

D

D

L

L

A

V

L

L

active site: R54 (= R55), T55 (= T56), K84 (= K85), R105 (= R106), H134 (= H135), Q137 (= Q138), T228 (= T229), P266 (= P267), G292 (= G293)
binding (s)-4-amino-4-oxo-3-(2-phosphonoacetamido)butanoic acid: S52 (= S53), T53 (= T54), R54 (= R55), T55 (= T56), R105 (= R106), H134 (= H135), R167 (= R168), R229 (= R230), L267 (= L268)

2fzkA The structure of wild-type e. Coli aspartate transcarbamoylase in complex with novel t state inhibitors at 2.50 resolution (see paper)
93% identity, 100% coverage: 2:311/311 of query aligns to 1:310/310 of 2fzkA

query
sites
2fzkA

A

A

N

N

P

P

L

L

Y

Y

Q

Q

K

K

H

H

I

I

I

I

S

S

I

I

N

N

D

D

L

L

S

S

R

R

E

D

D

D

L

L

E

N

L

L

V

V

L

L

A

A

T

T

A

A

A

A

K

K

L

L

K

K

A

A

H

N

P

P

Q

Q

P

P

E

E

L

L

L

L

K

K

H

H

K

K

V

V

I

I

A

A

S

S

C

C

F

F

F

F

E

E

A

A

S

S

T

T

R
|
R

T
|
T

R

R

L

L

S

S

F

F

E

E

T

T

S

S

M

M

H

H

R

R

L

L

G

G

A

A

S

S

V

V

V

V

G

G

F

F

S

S

D

D

S

S

S

A

N

N

T

T

S

S

L

L

G

G

K

K

K
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K

G

G

E

E

T

T

L

L

A

A

D

D

T

T

I

I

S

S

V

V

I

I

S

S

T

T

Y

Y

V

V

D

D

A

A

I

I

V

V

M

M

R
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R

H

H

P

P

Q

Q

E

E

G

G

A

A

A

A

R

R

L

L

A

A

T

T

E

E

F

F

S

S

G

G

G

N

I

V

P

P

V

V

L

L

N

N

A

A

G

G

D

D

G

G

A

S

N

N

Q

Q

H
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H

P

P

T

T

Q
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Q

T

T

L

L

L

L

D

D

L

L

F

F

T

T

I

I

Q

Q

E

E

T

T

Q

Q

G

G

R

R

L

L

E

D

N

N

L

L

N

H

V

V

A

A

M

M

V

V

G

G

D

D

L

L

K

K

Y

Y

G

G

R

R

T
|
T

V

V

H

H

S

S

L

L

T

T

Q

Q

A

A

L

L

A

A

K

K

F

F

N

D

G

G

N

N

R

R

F

F

Y

Y

F

F

I

I

A

A

P

P

D

D

A

A

L

L

A

A

M

M

P

P

Q

Q

Y

Y

I

I

L

L

D

D

M

M

L

L

D

D

D

E

K

K

G

G

I

I

A

A

W

W

S

S

L

L

H

H

S

S

A

S

I

I

E

E

E

E

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V

M

M

E

A

E

E

V

V

D

D

I

I

L

L

Y

Y

M

M

T
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T

R
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R

V

V

Q

Q

K

K

E

E

R

R

L

L

D

D

P

P

S

S

E

E

Y

Y

A

A

N

N

V

V

K

K

A

A

Q

Q

F

F

V

V

L

L

R

R

A

A

A

S

D

D

L

L

E

H

G

N

A

A

R

K

A

A

N

N

M

M

K

K

V

V

L

L

H

H

P
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P

L
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L

P

P

R

R

I

V

D

D

E

E

I

I

T

A

T

T

D

D

V

V

D

D

K

K

T

T

P

P

H

H

A

A

W

W

Y

Y

F

F

Q

Q

Q

Q

A

A

G

G

N

N

G
|
G

I

I

F

F

A

A

R
|
R

Q

Q

A

A

L

L

L

L

A

A

L

L

V

V

L

L

N

N

S

R

D

D

L

L

A

V

L

L

active site: R54 (= R55), T55 (= T56), K84 (= K85), R105 (= R106), H134 (= H135), Q137 (= Q138), T228 (= T229), P266 (= P267), G292 (= G293)
binding 3,5-bis[(phosphonoacetyl)amino]benzoic acid: T55 (= T56), H134 (= H135), Q137 (= Q138), T168 (= T169), R229 (= R230), P266 (= P267), L267 (= L268), R296 (= R297)

2fzgA The structure of wild-type e. Coli aspartate transcarbamoylase in complex with novel t state inhibitors at 2.25 resolution (see paper)
93% identity, 100% coverage: 2:311/311 of query aligns to 1:310/310 of 2fzgA

query
sites
2fzgA

A

A

N

N

P

P

L

L

Y

Y

Q

Q

K

K

H

H

I

I

I

I

S

S

I

I

N

N

D

D

L

L

S

S

R

R

E

D

D

D

L

L

E

N

L

L

V

V

L

L

A

A

T

T

A

A

A

A

K

K

L

L

K

K

A

A

H

N

P

P

Q

Q

P

P

E

E

L

L

L

L

K

K

H

H

K

K

V

V

I

I

A

A

S

S

C

C

F

F

F

F

E

E

A

A

S
|
S

T

T

R
|
R

T
|
T

R

R

L

L

S

S

F

F

E

E

T

T

S

S

M

M

H

H

R

R

L

L

G

G

A

A

S

S

V

V

V

V

G

G

F

F

S

S

D

D

S

S

S

A

N

N

T

T

S

S

L

L

G

G

K

K

K
|
K

G

G

E

E

T

T

L

L

A

A

D

D

T

T

I

I

S

S

V

V

I

I

S

S

T

T

Y

Y

V

V

D

D

A

A

I

I

V

V

M

M

R
|
R

H

H

P

P

Q

Q

E

E

G

G

A

A

A

A

R

R

L

L

A

A

T

T

E

E

F

F

S

S

G

G

G

N

I

V

P

P

V

V

L

L

N

N

A

A

G

G

D

D

G

G

A

S

N

N

Q

Q

H
|
H

P

P

T

T

Q
|
Q

T

T

L

L

L

L

D

D

L

L

F

F

T

T

I

I

Q

Q

E

E

T

T

Q

Q

G

G

R

R

L

L

E

D

N

N

L

L

N

H

V

V

A

A

M

M

V

V

G

G

D

D

L

L

K

K

Y

Y

G

G

R
|
R

T
|
T

V

V

H

H

S

S

L

L

T

T

Q

Q

A

A

L

L

A

A

K

K

F

F

N

D

G

G

N

N

R

R

F

F

Y

Y

F

F

I

I

A

A

P

P

D

D

A

A

L

L

A

A

M

M

P

P

Q

Q

Y

Y

I

I

L

L

D

D

M

M

L

L

D

D

D

E

K

K

G

G

I

I

A

A

W

W

S

S

L

L

H

H

S

S

A

S

I

I

E

E

E

E

V

V

M

M

E

A

E

E

V

V

D

D

I

I

L

L

Y

Y

M

M

T
|
T

R
|
R

V

V

Q

Q

K

K

E

E

R

R

L

L

D

D

P

P

S

S

E

E

Y

Y

A

A

N

N

V

V

K

K

A

A

Q

Q

F

F

V

V

L

L

R

R

A

A

A

S

D

D

L

L

E

H

G

N

A

A

R

K

A

A

N

N

M

M

K

K

V

V

L

L

H

H

P
|
P

L
|
L

P

P

R

R

I

V

D

D

E

E

I

I

T

A

T

T

D

D

V

V

D

D

K

K

T

T

P

P

H

H

A

A

W

W

Y

Y

F

F

Q

Q

Q

Q

A

A

G

G

N

N

G
|
G

I

I

F

F

A

A

R

R

Q

Q

A

A

L

L

L

L

A

A

L

L

V

V

L

L

N

N

S

R

D

D

L

L

A

V

L

L

active site: R54 (= R55), T55 (= T56), K84 (= K85), R105 (= R106), H134 (= H135), Q137 (= Q138), T228 (= T229), P266 (= P267), G292 (= G293)
binding {1,3-phenylenebis[imino(2-oxoethane-2,1-diyl)]}bis(phosphonic acid): S52 (= S53), R54 (= R55), T55 (= T56), R105 (= R106), H134 (= H135), R167 (= R168), T168 (= T169), R229 (= R230), P266 (= P267), L267 (= L268)

2fzcA The structure of wild-type e. Coli aspartate transcarbamoylase in complex with novel t state inhibitors at 2.10 resolution (see paper)
93% identity, 100% coverage: 2:311/311 of query aligns to 1:310/310 of 2fzcA

query
sites
2fzcA

A

A

N

N

P

P

L

L

Y

Y

Q

Q

K

K

H

H

I

I

I

I

S

S

I

I

N

N

D

D

L

L

S

S

R

R

E

D

D

D

L

L

E

N

L

L

V

V

L

L

A

A

T

T

A

A

A

A

K

K

L

L

K

K

A

A

H

N

P

P

Q

Q

P

P

E

E

L

L

L

L

K

K

H

H

K

K

V

V

I

I

A

A

S

S

C

C

F

F

F

F

E

E

A

A

S
|
S

T

T

R
|
R

T
|
T

R

R

L

L

S

S

F

F

E

E

T

T

S

S

M

M

H

H

R

R

L

L

G

G

A

A

S

S

V

V

V

V

G

G

F

F

S

S

D

D

S

S

S

A

N

N

T

T

S

S

L

L

G

G

K

K

K
|
K

G

G

E

E

T

T

L

L

A

A

D

D

T

T

I

I

S

S

V

V

I

I

S

S

T

T

Y

Y

V

V

D

D

A

A

I

I

V

V

M

M

R
|
R

H

H

P

P

Q

Q

E

E

G

G

A

A

A

A

R

R

L

L

A

A

T

T

E

E

F

F

S

S

G

G

G

N

I

V

P

P

V

V

L

L

N

N

A

A

G

G

D

D

G

G

A

S

N

N

Q

Q

H
|
H

P

P

T

T

Q
|
Q

T

T

L

L

L

L

D

D

L

L

F

F

T

T

I

I

Q

Q

E

E

T

T

Q

Q

G

G

R

R

L

L

E

D

N

N

L

L

N

H

V

V

A

A

M

M

V

V

G

G

D

D

L

L

K

K

Y

Y

G

G

R
|
R

T
|
T

V

V

H

H

S

S

L

L

T

T

Q

Q

A

A

L

L

A

A

K

K

F

F

N

D

G

G

N

N

R

R

F

F

Y

Y

F

F

I

I

A

A

P

P

D

D

A

A

L

L

A

A

M

M

P

P

Q

Q

Y

Y

I

I

L

L

D

D

M

M

L

L

D

D

D

E

K

K

G

G

I

I

A

A

W

W

S

S

L

L

H

H

S

S

A

S

I

I

E

E

E

E

V

V

M

M

E

A

E

E

V

V

D

D

I

I

L

L

Y

Y

M

M

T
|
T

R

R

V

V

Q

Q

K

K

E

E

R

R

L

L

D

D

P

P

S

S

E

E

Y

Y

A

A

N

N

V

V

K

K

A

A

Q

Q

F

F

V

V

L

L

R

R

A

A

A

S

D

D

L

L

E

H

G

N

A

A

R

K

A

A

N

N

M

M

K

K

V

V

L

L

H

H

P
|
P

L
|
L

P

P

R

R

I

V

D

D

E

E

I

I

T

A

T

T

D

D

V

V

D

D

K

K

T

T

P

P

H

H

A

A

W

W

Y

Y

F

F

Q

Q

Q

Q

A

A

G

G

N

N

G
|
G

I

I

F

F

A

A

R

R

Q

Q

A

A

L

L

L

L

A

A

L

L

V

V

L

L

N

N

S

R

D

D

L

L

A

V

L

L

active site: R54 (= R55), T55 (= T56), K84 (= K85), R105 (= R106), H134 (= H135), Q137 (= Q138), T228 (= T229), P266 (= P267), G292 (= G293)
binding {ethane-1,2-diylbis[imino(2-oxoethane-2,1-diyl)]}bis(phosphonic acid): S52 (= S53), R54 (= R55), T55 (= T56), R105 (= R106), R167 (= R168), T168 (= T169), P266 (= P267), L267 (= L268)

2airA T-state active site of aspartate transcarbamylase:crystal structure of the carbamyl phosphate and l-alanosine ligated enzyme (see paper)
93% identity, 100% coverage: 2:311/311 of query aligns to 1:310/310 of 2airA

query
sites
2airA

A

A

N

N

P

P

L

L

Y

Y

Q

Q

K

K

H

H

I

I

I

I

S

S

I

I

N

N

D

D

L

L

S

S

R

R

E

D

D

D

L

L

E

N

L

L

V

V

L

L

A

A

T

T

A

A

A

A

K

K

L

L

K

K

A

A

H

N

P

P

Q

Q

P

P

E

E

L

L

L

L

K

K

H

H

K

K

V

V

I

I

A

A

S

S

C

C

F

F

F

F

E

E

A

A

S
|
S

T
|
T

R
|
R

T
|
T

R

R

L

L

S

S

F

F

E

E

T

T

S

S

M

M

H

H

R

R

L

L

G

G

A

A

S

S

V

V

V

V

G

G

F

F

S

S

D

D

S

S

S

A

N

N

T

T

S

S

L

L

G

G

K

K

K
|
K

G

G

E

E

T

T

L

L

A

A

D

D

T

T

I

I

S

S

V

V

I

I

S

S

T

T

Y

Y

V

V

D

D

A

A

I

I

V

V

M

M

R
|
R

H

H

P

P

Q

Q

E

E

G

G

A

A

A

A

R

R

L

L

A

A

T

T

E

E

F

F

S

S

G

G

G

N

I

V

P

P

V

V

L

L

N

N

A

A

G

G

D

D

G

G

A

S

N

N

Q

Q

H
|
H

P

P

T

T

Q
|
Q

T

T

L

L

L

L

D

D

L

L

F

F

T

T

I

I

Q

Q

E

E

T

T

Q

Q

G

G

R

R

L

L

E

D

N

N

L

L

N

H

V

V

A

A

M

M

V

V

G

G

D

D

L

L

K

K

Y

Y

G

G

R

R

T

T

V

V

H

H

S

S

L

L

T

T

Q

Q

A

A

L

L

A

A

K

K

F

F

N

D

G

G

N

N

R

R

F

F

Y

Y

F

F

I

I

A

A

P

P

D

D

A

A

L

L

A

A

M

M

P

P

Q

Q

Y

Y

I

I

L

L

D

D

M

M

L

L

D

D

D

E

K

K

G

G

I

I

A

A

W

W

S

S

L

L

H

H

S

S

A

S

I

I

E

E

E

E

V

V

M

M

E

A

E

E

V

V

D

D

I

I

L

L

Y

Y

M

M

T
|
T

R

R

V

V

Q

Q

K

K

E

E

R

R

L

L

D

D

P

P

S

S

E

E

Y

Y

A

A

N

N

V

V

K

K

A

A

Q

Q

F

F

V

V

L

L

R

R

A

A

A

S

D

D

L

L

E

H

G

N

A

A

R

K

A

A

N

N

M

M

K

K

V

V

L

L

H

H

P
|
P

L

L

P

P

R

R

I

V

D

D

E

E

I

I

T

A

T

T

D

D

V

V

D

D

K

K

T

T

P

P

H

H

A

A

W

W

Y

Y

F

F

Q

Q

Q

Q

A

A

G

G

N

N

G
|
G

I

I

F

F

A

A

R

R

Q

Q

A

A

L

L

L

L

A

A

L

L

V

V

L

L

N

N

S

R

D

D

L

L

A

V

L

L

active site: R54 (= R55), T55 (= T56), K84 (= K85), R105 (= R106), H134 (= H135), Q137 (= Q138), T228 (= T229), P266 (= P267), G292 (= G293)
binding 3-[hydroxy(nitroso)amino]-l-alanine: S52 (= S53), T53 (= T54), R54 (= R55), R105 (= R106)
binding phosphoric acid mono(formamide)ester: R54 (= R55), T55 (= T56), R105 (= R106), H134 (= H135)

1za2A Structure of wild-type e. Coli aspartate transcarbamoylase in the presence of ctp, carbamoyl phosphate at 2.50 a resolution (see paper)
93% identity, 100% coverage: 2:311/311 of query aligns to 1:310/310 of 1za2A

query
sites
1za2A

A

A

N

N

P

P

L

L

Y

Y

Q

Q

K

K

H

H

I

I

I

I

S

S

I

I

N

N

D

D

L

L

S

S

R

R

E

D

D

D

L

L

E

N

L

L

V

V

L

L

A

A

T

T

A

A

A

A

K

K

L

L

K

K

A

A

H

N

P

P

Q

Q

P

P

E

E

L

L

L

L

K

K

H

H

K

K

V

V

I

I

A

A

S

S

C

C

F

F

F

F

E

E

A

A

S

S

T
|
T

R
|
R

T
|
T

R

R

L

L

S

S

F

F

E

E

T

T

S

S

M

M

H

H

R

R

L

L

G

G

A

A

S

S

V

V

V

V

G

G

F

F

S

S

D

D

S

S

S

A

N

N

T

T

S

S

L

L

G

G

K

K

K
|
K

G

G

E

E

T

T

L

L

A

A

D

D

T

T

I

I

S

S

V

V

I

I

S

S

T

T

Y

Y

V

V

D

D

A

A

I

I

V

V

M

M

R
|
R

H

H

P

P

Q

Q

E

E

G

G

A

A

A

A

R

R

L

L

A

A

T

T

E

E

F

F

S

S

G

G

G

N

I

V

P

P

V

V

L

L

N

N

A

A

G

G

D

D

G

G

A

S

N

N

Q

Q

H
|
H

P

P

T

T

Q
|
Q

T

T

L

L

L

L

D

D

L

L

F

F

T

T

I

I

Q

Q

E

E

T

T

Q

Q

G

G

R

R

L

L

E

D

N

N

L

L

N

H

V

V

A

A

M

M

V

V

G

G

D

D

L

L

K

K

Y

Y

G

G

R
|
R

T
|
T

V

V

H

H

S

S

L

L

T

T

Q

Q

A

A

L

L

A

A

K

K

F

F

N

D

G

G

N

N

R

R

F

F

Y

Y

F

F

I

I

A

A

P

P

D

D

A

A

L

L

A

A

M

M

P

P

Q

Q

Y

Y

I

I

L

L

D

D

M

M

L

L

D

D

D

E

K

K

G

G

I

I

A

A

W

W

S

S

L

L

H

H

S

S

A

S

I

I

E

E

E

E

V

V

M

M

E

A

E

E

V

V

D

D

I

I

L

L

Y

Y

M

M

T
|
T

R

R

V

V

Q

Q

K

K

E

E

R

R

L

L

D

D

P

P

S

S

E

E

Y

Y

A

A

N

N

V

V

K

K

A

A

Q

Q

F

F

V

V

L

L

R

R

A

A

A

S

D

D

L

L

E

H

G

N

A

A

R

K

A

A

N

N

M

M

K

K

V

V

L

L

H

H

P
|
P

L
|
L

P

P

R

R

I

V

D

D

E

E

I

I

T

A

T

T

D

D

V

V

D

D

K

K

T

T

P

P

H

H

A

A

W

W

Y

Y

F

F

Q

Q

Q

Q

A

A

G

G

N

N

G
|
G

I

I

F

F

A

A

R

R

Q

Q

A

A

L

L

L

L

A

A

L

L

V

V

L

L

N

N

S

R

D

D

L

L

A

V

L

L

active site: R54 (= R55), T55 (= T56), K84 (= K85), R105 (= R106), H134 (= H135), Q137 (= Q138), T228 (= T229), P266 (= P267), G292 (= G293)
binding phosphoric acid mono(formamide)ester: T53 (= T54), R54 (= R55), T55 (= T56), R105 (= R106), R167 (= R168), T168 (= T169), L267 (= L268)

1r0cA Products in the t state of aspartate transcarbamylase: crystal structure of the phosphate and n-carbamyl-l-aspartate ligated enzyme (see paper)
93% identity, 100% coverage: 2:311/311 of query aligns to 1:310/310 of 1r0cA

query
sites
1r0cA

A

A

N

N

P

P

L

L

Y

Y

Q

Q

K

K

H

H

I

I

I

I

S

S

I

I

N

N

D

D

L

L

S

S

R

R

E

D

D

D

L

L

E

N

L

L

V

V

L

L

A

A

T

T

A

A

A

A

K

K

L

L

K

K

A

A

H

N

P

P

Q

Q

P

P

E

E

L

L

L

L

K

K

H

H

K

K

V

V

I

I

A

A

S

S

C

C

F

F

F

F

E

E

A

A

S
|
S

T

T

R
|
R

T
|
T

R

R

L

L

S

S

F

F

E

E

T

T

S

S

M

M

H

H

R

R

L

L

G

G

A

A

S

S

V

V

V

V

G

G

F

F

S

S

D

D

S

S

S

A

N

N

T

T

S

S

L

L

G

G

K

K

K
|
K

G

G

E

E

T

T

L

L

A

A

D

D

T

T

I

I

S

S

V

V

I

I

S

S

T

T

Y

Y

V

V

D

D

A

A

I

I

V

V

M

M

R
|
R

H

H

P

P

Q

Q

E

E

G

G

A

A

A

A

R

R

L

L

A

A

T

T

E

E

F

F

S

S

G

G

G

N

I

V

P

P

V

V

L

L

N

N

A

A

G

G

D

D

G

G

A

S

N

N

Q

Q

H
|
H

P

P

T

T

Q
|
Q

T

T

L

L

L

L

D

D

L

L

F

F

T

T

I

I

Q

Q

E

E

T

T

Q

Q

G

G

R

R

L

L

E

D

N

N

L

L

N

H

V

V

A

A

M

M

V

V

G

G

D

D

L

L

K

K

Y

Y

G

G

R

R

T

T

V

V

H

H

S

S

L

L

T

T

Q

Q

A

A

L

L

A

A

K

K

F

F

N

D

G

G

N

N

R

R

F

F

Y

Y

F

F

I

I

A

A

P

P

D

D

A

A

L

L

A

A

M

M

P

P

Q

Q

Y

Y

I

I

L

L

D

D

M

M

L

L

D

D

D

E

K

K

G

G

I

I

A

A

W

W

S

S

L

L

H

H

S

S

A

S

I

I

E

E

E

E

V

V

M

M

E

A

E

E

V

V

D

D

I

I

L

L

Y

Y

M

M

T
|
T

R

R

V

V

Q

Q

K

K

E

E

R

R

L

L

D

D

P

P

S

S

E

E

Y

Y

A

A

N

N

V

V

K

K

A

A

Q

Q

F

F

V

V

L

L

R

R

A

A

A

S

D

D

L

L

E

H

G

N

A

A

R

K

A

A

N

N

M

M

K

K

V

V

L

L

H

H

P
|
P

L

L

P

P

R

R

I

V

D

D

E

E

I

I

T

A

T

T

D

D

V

V

D

D

K

K

T

T

P

P

H

H

A

A

W

W

Y

Y

F

F

Q

Q

Q

Q

A

A

G

G

N

N

G
|
G

I

I

F

F

A

A

R

R

Q

Q

A

A

L

L

L

L

A

A

L

L

V

V

L

L

N

N

S

R

D

D

L

L

A

V

L

L

active site: R54 (= R55), T55 (= T56), K84 (= K85), R105 (= R106), H134 (= H135), Q137 (= Q138), T228 (= T229), P266 (= P267), G292 (= G293)
binding n-carbamoyl-l-aspartate: S52 (= S53), R54 (= R55), R105 (= R106)
binding phosphate ion: R105 (= R106), H134 (= H135), Q137 (= Q138)

1r0bA Aspartate transcarbamylase (atcase) of escherichia coli: a new crystalline r state bound to pala, or to product analogues phosphate and citrate (see paper)
93% identity, 100% coverage: 2:311/311 of query aligns to 1:310/310 of 1r0bA

query
sites
1r0bA

A

A

N

N

P

P

L

L

Y

Y

Q

Q

K

K

H

H

I

I

I

I

S

S

I

I

N

N

D

D

L

L

S

S

R

R

E

D

D

D

L

L

E

N

L

L

V

V

L

L

A

A

T

T

A

A

A

A

K

K

L

L

K

K

A

A

H

N

P

P

Q

Q

P

P

E

E

L

L

L

L

K

K

H

H

K

K

V

V

I

I

A

A

S

S

C

C

F

F

F

F

E

E

A

A

S

S

T

T

R
|
R

T
|
T

R

R

L

L

S

S

F

F

E

E

T

T

S

S

M

M

H

H

R

R

L

L

G

G

A

A

S

S

V

V

V

V

G

G

F

F

S

S

D

D

S

S

S

A

N

N

T

T

S
|
S

L

L

G

G

K

K

K
|
K

G

G

E

E

T

T

L

L

A

A

D

D

T

T

I

I

S

S

V

V

I

I

S

S

T

T

Y

Y

V

V

D

D

A

A

I

I

V

V

M

M

R
|
R

H

H

P

P

Q

Q

E

E

G

G

A

A

A

A

R

R

L

L

A

A

T

T

E

E

F

F

S

S

G

G

G

N

I

V

P

P

V

V

L

L

N

N

A

A

G

G

D

D

G

G

A

S

N

N

Q

Q

H
|
H

P

P

T

T

Q
|
Q

T

T

L

L

L

L

D

D

L

L

F

F

T

T

I

I

Q

Q

E

E

T

T

Q

Q

G

G

R

R

L

L

E

D

N

N

L

L

N

H

V

V

A

A

M

M

V

V

G

G

D

D

L

L

K

K

Y

Y

G

G

R
|
R

T

T

V

V

H

H

S

S

L

L

T

T

Q

Q

A

A

L

L

A

A

K

K

F

F

N

D

G

G

N

N

R

R

F

F

Y

Y

F

F

I

I

A

A

P

P

D

D

A

A

L

L

A

A

M

M

P

P

Q

Q

Y

Y

I

I

L

L

D

D

M

M

L

L

D

D

D

E

K

K

G

G

I

I

A

A

W

W

S

S

L

L

H

H

S

S

A

S

I

I

E

E

E

E

V

V

M

M

E

A

E

E

V

V

D

D

I

I

L

L

Y

Y

M

M

T
|
T

R
|
R

V

V

Q
|
Q

K

K

E

E

R

R

L

L

D

D

P

P

S

S

E

E

Y

Y

A

A

N

N

V

V

K

K

A

A

Q

Q

F

F

V

V

L

L

R

R

A

A

A

S

D

D

L

L

E

H

G

N

A

A

R

K

A

A

N

N

M

M

K

K

V

V

L

L

H

H

P
|
P

L

L

P
|
P

R

R

I

V

D

D

E

E

I

I

T

A

T

T

D

D

V

V

D

D

K

K

T

T

P

P

H

H

A

A

W

W

Y

Y

F

F

Q

Q

Q

Q

A

A

G

G

N

N

G
|
G

I

I

F

F

A

A

R

R

Q

Q

A

A

L

L

L

L

A

A

L

L

V

V

L

L

N

N

S

R

D

D

L

L

A

V

L

L

active site: R54 (= R55), T55 (= T56), K84 (= K85), R105 (= R106), H134 (= H135), Q137 (= Q138), T228 (= T229), P266 (= P267), G292 (= G293)
binding citrate anion: H134 (= H135), R167 (= R168), R229 (= R230), Q231 (= Q232), P266 (= P267), P268 (= P269)
binding phosphate ion: S80 (= S81), K84 (= K85)

2hseA Structure of d236a e. Coli aspartate transcarbamoylase in the presence of phosphonoacetamide and l-aspartate at 2.60 a resolution
93% identity, 100% coverage: 2:311/311 of query aligns to 1:310/310 of 2hseA

query
sites
2hseA

A

A

N

N

P

P

L

L

Y

Y

Q

Q

K

K

H

H

I

I

I

I

S

S

I

I

N

N

D

D

L

L

S

S

R

R

E

D

D

D

L

L

E

N

L

L

V

V

L

L

A

A

T

T

A

A

A

A

K

K

L

L

K

K

A

A

H

N

P

P

Q

Q

P

P

E

E

L

L

L

L

K

K

H

H

K

K

V

V

I

I

A

A

S

S

C

C

F

F

F

F

E

E

A

A

S
|
S

T
|
T

R
|
R

T
|
T

R

R

L

L

S
|
S

F

F

E

E

T

T

S

S

M

M

H

H

R

R

L

L

G

G

A

A

S

S

V

V

V

V

G

G

F

F

S

S

D

D

S

S

S

A

N

N

T

T

S

S

L

L

G

G

K

K

K
|
K

G

G

E

E

T

T

L

L

A

A

D

D

T

T

I

I

S

S

V

V

I

I

S

S

T

T

Y

Y

V

V

D

D

A

A

I

I

V

V

M

M

R
|
R

H

H

P

P

Q

Q

E

E

G

G

A

A

A

A

R

R

L

L

A

A

T

T

E

E

F

F

S

S

G

G

G

N

I

V

P

P

V

V

L

L

N

N

A

A

G

G

D

D

G

G

A

S

N

N

Q

Q

H
|
H

P

P

T

T

Q
|
Q

T

T

L

L

L

L

D

D

L

L

F

F

T

T

I

I

Q

Q

E

E

T

T

Q

Q

G

G

R

R

L

L

E

D

N

N

L

L

N

H

V

V

A

A

M

M

V

V

G

G

D

D

L

L

K

K

Y

Y

G

G

R
|
R

T

T

V

V

H

H

S

S

L

L

T

T

Q

Q

A

A

L

L

A

A

K

K

F

F

N

D

G

G

N

N

R

R

F

F

Y

Y

F

F

I

I

A

A

P

P

D

D

A

A

L

L

A

A

M

M

P

P

Q

Q

Y

Y

I

I

L

L

D

D

M

M

L

L

D

D

D

E

K

K

G

G

I

I

A

A

W

W

S

S

L

L

H

H

S

S

A

S

I

I

E

E

E

E

V

V

M

M

E

A

E

E

V

V

D

D

I

I

L

L

Y

Y

M

M

T
|
T

R
|
R

V

V

Q
|
Q

K

K

E

E

R

R

L

L

D

A

P

P

S

S

E

E

Y

Y

A

A

N

N

V

V

K

K

A

A

Q

Q

F

F

V

V

L

L

R

R

A

A

A

S

D

D

L

L

E

H

G

N

A

A

R

K

A

A

N

N

M

M

K

K

V

V

L

L

H

H

P
|
P

L
|
L

P
|
P

R

R

I

V

D

D

E

E

I

I

T

A

T

T

D

D

V

V

D

D

K

K

T

T

P

P

H

H

A

A

W

W

Y

Y

F

F

Q

Q

Q

Q

A
|
A

G

G

N

N

G
|
G

I

I

F

F

A

A

R
|
R

Q

Q

A

A

L

L

L

L

A

A

L

L

V

V

L

L

N

N

S

R

D

D

L

L

A

V

L

L

active site: R54 (= R55), T55 (= T56), K84 (= K85), R105 (= R106), H134 (= H135), Q137 (= Q138), T228 (= T229), P266 (= P267), G292 (= G293)
binding aspartic acid: R54 (= R55), T55 (= T56), S58 (= S59), R105 (= R106), H134 (= H135), Q137 (= Q138), R167 (= R168), R229 (= R230), Q231 (= Q232), L267 (= L268), P268 (= P269), A289 (= A290), R296 (= R297)
binding phosphonoacetamide: S52 (= S53), T53 (= T54), R54 (= R55), T55 (= T56), R105 (= R106), L267 (= L268)

2a0fA Structure of d236a mutant e. Coli aspartate transcarbamoylase in presence of phosphonoacetamide at 2.90 a resolution (see paper)
93% identity, 100% coverage: 2:311/311 of query aligns to 1:310/310 of 2a0fA

query
sites
2a0fA

A

A

N

N

P

P

L

L

Y

Y

Q

Q

K

K

H

H

I

I

I

I

S

S

I

I

N

N

D

D

L

L

S

S

R

R

E

D

D

D

L

L

E

N

L

L

V

V

L

L

A

A

T

T

A

A

A

A

K

K

L

L

K

K

A

A

H

N

P

P

Q

Q

P

P

E

E

L

L

L

L

K

K

H

H

K

K

V

V

I

I

A

A

S

S

C

C

F

F

F

F

E

E

A

A

S

S

T

T

R
|
R

T
|
T

R

R

L

L

S

S

F

F

E

E

T

T

S

S

M

M

H

H

R

R

L

L

G

G

A

A

S

S

V

V

V

V

G

G

F

F

S

S

D

D

S

S

S

A

N

N

T

T

S

S

L

L

G

G

K

K

K
|
K

G

G

E

E

T

T

L

L

A

A

D

D

T

T

I

I

S

S

V

V

I

I

S

S

T

T

Y

Y

V

V

D

D

A

A

I

I

V

V

M

M

R
|
R

H

H

P

P

Q

Q

E

E

G

G

A

A

A

A

R

R

L

L

A

A

T

T

E

E

F

F

S

S

G

G

G

N

I

V

P

P

V

V

L

L

N

N

A

A

G

G

D

D

G

G

A

S

N

N

Q

Q

H
|
H

P

P

T

T

Q
|
Q

T

T

L

L

L

L

D

D

L

L

F

F

T

T

I

I

Q

Q

E

E

T

T

Q

Q

G

G

R

R

L

L

E

D

N

N

L

L

N

H

V

V

A

A

M

M

V

V

G

G

D

D

L

L

K

K

Y

Y

G

G

R

R

T

T

V

V

H

H

S

S

L

L

T

T

Q

Q

A

A

L

L

A

A

K

K

F

F

N

D

G

G

N

N

R

R

F

F

Y

Y

F

F

I

I

A

A

P

P

D

D

A

A

L

L

A

A

M

M

P

P

Q

Q

Y

Y

I

I

L

L

D

D

M

M

L

L

D

D

D

E

K

K

G

G

I

I

A

A

W

W

S

S

L

L

H

H

S

S

A

S

I

I

E

E

E

E

V

V

M

M

E

A

E

E

V

V

D

D

I

I

L

L

Y

Y

M

M

T
|
T

R

R

V

V

Q

Q

K

K

E

E

R

R

L

L

D

A

P

P

S

S

E

E

Y

Y

A

A

N

N

V

V

K

K

A

A

Q

Q

F

F

V

V

L

L

R

R

A

A

A

S

D

D

L

L

E

H

G

N

A

A

R

K

A

A

N

N

M

M

K

K

V

V

L

L

H

H

P
|
P

L
|
L

P

P

R

R

I

V

D

D

E

E

I

I

T

A

T

T

D

D

V

V

D

D

K

K

T

T

P

P

H

H

A

A

W

W

Y

Y

F

F

Q

Q

Q

Q

A

A

G

G

N

N

G
|
G

I

I

F

F

A

A

R

R

Q

Q

A

A

L

L

L

L

A

A

L

L

V

V

L

L

N

N

S

R

D

D

L

L

A

V

L

L

active site: R54 (= R55), T55 (= T56), K84 (= K85), R105 (= R106), H134 (= H135), Q137 (= Q138), T228 (= T229), P266 (= P267), G292 (= G293)
binding phosphonoacetamide: R54 (= R55), T55 (= T56), H134 (= H135), Q137 (= Q138), L267 (= L268)

5vmqC Structure of the r105a mutant catalytic trimer of escherichia coli aspartate transcarbamoylase at 2.0-a resolution (see paper)
93% identity, 99% coverage: 2:309/311 of query aligns to 1:308/309 of 5vmqC

query
sites
5vmqC

A

A

N

N

P

P

L

L

Y

Y

Q

Q

K

K

H

H

I

I

I

I

S

S

I

I

N

N

D

D

L

L

S

S

R

R

E

D

D

D

L

L

E

N

L

L

V

V

L

L

A

A

T

T

A

A

A

A

K

K

L

L

K

K

A

A

H

N

P

P

Q

Q

P

P

E

E

L

L

L

L

K

K

H

H

K

K

V

V

I

I

A

A

S

S

C

C

F

F

F

F

E

E

A

A

S

S

T

T

R
|
R

T
|
T

R

R

L

L

S

S

F

F

E

E

T

T

S

S

M

M

H

H

R

R

L

L

G

G

A

A

S

S

V

V

V

V

G

G

F

F

S

S

D

D

S

S

S

A

N

N

T

T

S

S

L

L

G

G

K

K

K
|
K

G

G

E

E

T

T

L

L

A

A

D

D

T

T

I

I

S

S

V

V

I

I

S

S

T

T

Y

Y

V

V

D

D

A

A

I

I

V

V

M

M

R
x
A

H

H

P

P

Q

Q

E

E

G

G

A

A

A

A

R

R

L

L

A

A

T

T

E

E

F

F

S

S

G

G

G

N

I

V

P

P

V

V

L

L

N

N

A

A

G

G

D

D

G

G

A

S

N

N

Q

Q

H
|
H

P

P

T

T

Q
|
Q

T

T

L

L

L

L

D

D

L

L

F

F

T

T

I

I

Q

Q

E

E

T

T

Q

Q

G

G

R

R

L

L

E

D

N

N

L

L

N

H

V

V

A

A

M

M

V

V

G

G

D

D

L

L

K

K

Y

Y

G

G

R

R

T

T

V

V

H

H

S

S

L

L

T

T

Q

Q

A

A

L

L

A

A

K

K

F

F

N

D

G

G

N

N

R

R

F

F

Y

Y

F

F

I

I

A

A

P

P

D

D

A

A

L

L

A

A

M

M

P

P

Q

Q

Y

Y

I

I

L

L

D

D

M

M

L

L

D

D

D

E

K

K

G

G

I

I

A

A

W

W

S

S

L

L

H

H

S

S

A

S

I

I

E
|
E

E

E

V

V

M

M

E

A

E

E

V

V

D

D

I

I

L

L

Y

Y

M

M

T
|
T

R

R

V

V

Q

Q

K

K

E

E

R

R

L

L

D

D

P

P

S

S

E

E

Y

Y

A

A

N

N

V

V

K

K

A

A

Q

Q

F

F

V

V

L

L

R

R

A

A

A

S

D
|
D

L

L

E

H

G

N

A

A

R

K

A

A

N

N

M

M

K

K

V

V

L

L

H

H

P
|
P

L

L

P

P

R

R

I

V

D

D

E

E

I

I

T

A

T

T

D

D

V

V

D

D

K

K

T

T

P

P

H

H

A

A

W

W

Y

Y

F

F

Q

Q

Q

Q

A

A

G

G

N

N

G
|
G

I

I

F

F

A

A

R

R

Q

Q

A

A

L

L

L

L

A

A

L

L

V

V

L

L

N

N

S

R

D

D

L

L

active site: R54 (= R55), T55 (= T56), K84 (= K85), A105 (≠ R106), H134 (= H135), Q137 (= Q138), T228 (= T229), P266 (= P267), G292 (= G293)
binding calcium ion: E216 (= E217), D253 (= D254)

2at1A Crystal structures of phosphonoacetamide ligated t and phosphonoacetamide and malonate ligated r states of aspartate carbamoyltransferase at 2.8-angstroms resolution and neutral ph (see paper)
92% identity, 100% coverage: 2:311/311 of query aligns to 1:310/310 of 2at1A

query
sites
2at1A

A

A

N

N

P

P

L

L

Y

Y

Q

Q

K

K

H

H

I

I

I

I

S

S

I

I

N

N

D

D

L

L

S

S

R

R

E

D

D

D

L

L

E

N

L

L

V

V

L

L

A

A

T

T

A

A

A

A

K

K

L

L

K

K

A

A

H

N

P

P

Q

Q

P

P

E

E

L

L

L

L

K

K

H

H

K

K

V

V

I

I

A

A

S

S

C

C

F

F

F

F

E

E

A

A

S
|
S

T
|
T

R
|
R

T
|
T

R

R

L

L

S

S

F

F

E

Q

T

T

S

S

M

M

H

H

R

R

L

L

G

G

A

A

S

S

V

V

V

V

G

G

F

F

S

S

D

D

S

S

S

A

N

N

T

T

S

S

L

L

G

G

K

K

K
|
K

G

G

E

E

T

T

L

L

A

A

D

D

T

T

I

I

S

S

V

V

I

I

S

S

T

T

Y

Y

V

V

D

D

A

A

I

I

V

V

M

M

R
|
R

H

H

P

P

Q

Q

E

E

G

G

A

A

A

A

R

R

L

L

A

A

T

T

E

E

F

F

S

S

G

G

G

N

I

V

P

P

V

V

L

L

N

N

A

A

G

G

D

D

G

G

A

S

N

N

Q

Q

H
|
H

P

P

T

T

Q
|
Q

T

T

L

L

L

L

D

D

L

L

F

F

T

T

I

I

Q

Q

E

Q

T

T

Q

E

G

G

R

R

L

L

E

D

N

N

L

L

N

H

V

V

A

A

M

M

V

V

G

G

D

D

L

L

K

K

Y

Y

G

G

R
|
R

T

T

V

V

H

H

S

S

L

L

T

T

Q

Q

A

A

L

L

A

A

K

K

F

F

N

D

G

G

N

N

R

R

F

F

Y

Y

F

F

I

I

A

A

P

P

D

D

A

A

L

L

A

A

M

M

P

P

Q

E

Y

Y

I

I

L

L

D

D

M

M

L

L

D

D

D

E

K

K

G

G

I

I

A

A

W

W

S

S

L

L

H

H

S

S

A

S

I

I

E

E

E

E

V

V

M

M

E

A

E

E

V

V

D

D

I

I

L

L

Y

Y

M

M

T
|
T

R
|
R

V

V

Q

Q

K

K

E

E

R

R

L

L

D

D

P

P

S

S

E

E

Y

Y

A

A

N

N

V

V

K

K

A

A

Q

Q

F

F

V

V

L

L

R

R

A

A

A

S

D

D

L

L

E

H

G

N

A

A

R

K

A

A

N

N

M

M

K

K

V

V

L

L

H

H

P
|
P

L

L

P

P

R

R

I

V

D

D

E

E

I

I

T

A

T

T

D

D

V

V

D

D

K

K

T

T

P

P

H

H

A

A

W

W

Y

Y

F

F

Q

Q

Q

Q

A

A

G

G

N

N