SitesBLAST
Comparing BWI76_RS03140 BWI76_RS03140 alcohol dehydrogenase to proteins with known functional sites using BLASTp with E ≤ 0.001.
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Found 20 (the maximum) hits to proteins with known functional sites (download)
7bu2B Structure of alcohol dehydrogenase yjgb from escherichia coli (see paper)
85% identity, 99% coverage: 4:339/339 of query aligns to 2:337/339 of 7bu2B
7bu3A Structure of alcohol dehydrogenase yjgb in complex with NADP from escherichia coli (see paper)
85% identity, 99% coverage: 2:337/339 of query aligns to 1:336/336 of 7bu3A
- binding aspartic acid: S314 (= S315), A336 (= A337)
- binding nadp nicotinamide-adenine-dinucleotide phosphate: C40 (= C41), H41 (= H42), S42 (= S43), W51 (= W52), C151 (= C152), T155 (= T156), G175 (= G176), I176 (= I177), G177 (= G178), G178 (= G179), L179 (= L180), S198 (= S199), S199 (= S200), K203 (= K204), T237 (= T238), V238 (= V239), V240 (= V241), V260 (= V261), G261 (= G262), A285 (= A286), T286 (= T287), R331 (= R332)
- binding zinc ion: C40 (= C41), H62 (= H63), E63 (= E64), C95 (= C96), C98 (= C99), C101 (= C102), C109 (= C110), C151 (= C152)
6c49A Crystal structure of alcohol dehydrogenase from acinetobacter baumannii
60% identity, 100% coverage: 2:339/339 of query aligns to 2:336/338 of 6c49A
P9WQC5 NADP-dependent alcohol dehydrogenase C; EC 1.1.1.2 from Mycobacterium tuberculosis (strain ATCC 25618 / H37Rv) (see paper)
37% identity, 99% coverage: 1:335/339 of query aligns to 1:341/346 of P9WQC5
- K209 (≠ A203) modified: Isoglutamyl lysine isopeptide (Lys-Gln) (interchain with Q-Cter in protein Pup)
P0CH37 NADP-dependent alcohol dehydrogenase C 2; Ms-ADHC 2; EC 1.1.1.2 from Mycolicibacterium smegmatis (strain ATCC 700084 / mc(2)155) (Mycobacterium smegmatis) (see paper)
38% identity, 99% coverage: 1:335/339 of query aligns to 1:342/349 of P0CH37
- K210 (≠ A203) modified: Isoglutamyl lysine isopeptide (Lys-Gln) (interchain with Q-Cter in protein Pup)
P0CH36 NADP-dependent alcohol dehydrogenase C 1; Ms-ADHC 1; EC 1.1.1.2 from Mycolicibacterium smegmatis (strain ATCC 700084 / mc(2)155) (Mycobacterium smegmatis) (see paper)
38% identity, 99% coverage: 1:335/339 of query aligns to 1:342/349 of P0CH36
- K210 (≠ A203) modified: Isoglutamyl lysine isopeptide (Lys-Gln) (interchain with Q-Cter in protein Pup)
1yqdA Sinapyl alcohol dehydrogenase complexed with NADP+ (see paper)
36% identity, 97% coverage: 7:335/339 of query aligns to 13:348/359 of 1yqdA
- active site: C47 (= C41), H48 (= H42), S49 (= S43), H52 (≠ S46), H69 (= H63), E70 (= E64), C100 (= C96), C103 (= C99), C106 (= C102), C114 (≠ V115), I118 (= I119), C163 (= C152), T167 (= T156), R345 (= R332)
- binding nadp nicotinamide-adenine-dinucleotide phosphate: C47 (= C41), H48 (= H42), S49 (= S43), H52 (≠ S46), C163 (= C152), T167 (= T156), G188 (= G176), L189 (≠ I177), G190 (= G178), G191 (= G179), L192 (= L180), S211 (= S199), T212 (≠ S200), S213 (≠ N201), K216 (= K204), T251 (= T238), V252 (= V239), S253 (≠ A240), V274 (= V261), G275 (= G262), A276 (= A263), G299 (≠ A286), I300 (≠ T287), N340 (≠ G327), R345 (= R332)
- binding zinc ion: C47 (= C41), H69 (= H63), C100 (= C96), C103 (= C99), C106 (= C102), C114 (≠ V115), C163 (= C152)
6k3gB Crystal structure of 10-hydroxygeraniol dehydrogenase from cantharanthus roseus in complex with NADP+ (see paper)
39% identity, 97% coverage: 7:335/339 of query aligns to 13:348/356 of 6k3gB
- active site: C47 (= C41), S49 (= S43), H52 (≠ S46), H69 (= H63), C163 (= C152)
- binding nadp nicotinamide-adenine-dinucleotide phosphate: H48 (= H42), S49 (= S43), H52 (≠ S46), W58 (= W52), C163 (= C152), T167 (= T156), G188 (= G176), L189 (≠ I177), G190 (= G178), G191 (= G179), L192 (= L180), S211 (= S199), T212 (≠ S200), S213 (≠ N201), K216 (= K204), T251 (= T238), V252 (= V239), S253 (≠ A240), A254 (≠ V241), V274 (= V261), G275 (= G262), A276 (= A263), A299 (= A286), I300 (≠ T287), R345 (= R332)
- binding zinc ion: C47 (= C41), H69 (= H63), C100 (= C96), C103 (= C99), C106 (= C102), C114 (≠ L111), C163 (= C152)
5vktA Cinnamyl alcohol dehydrogenases (sbcad4) from sorghum bicolor (l.) Moench (see paper)
35% identity, 98% coverage: 3:335/339 of query aligns to 2:343/353 of 5vktA
- active site: C40 (= C41), H41 (= H42), T42 (≠ S43), H45 (≠ S46), H62 (= H63), E63 (= E64), C93 (= C96), C96 (= C99), C99 (= C102), C107 (= C110), V111 (≠ S114), C158 (= C152), T162 (= T156), R340 (= R332)
- binding nadp nicotinamide-adenine-dinucleotide phosphate: C40 (= C41), H41 (= H42), T42 (≠ S43), H45 (≠ S46), C158 (= C152), T162 (= T156), G183 (= G176), L184 (≠ I177), G185 (= G178), G186 (= G179), L187 (= L180), S206 (= S199), S207 (= S200), S208 (≠ N201), K211 (= K204), T246 (= T238), V247 (= V239), S248 (≠ A240), A249 (≠ V241), V269 (= V261), G270 (= G262), N293 (≠ S285), G294 (≠ A286), N335 (≠ G327), R340 (= R332)
- binding zinc ion: C40 (= C41), H62 (= H63), C93 (= C96), C96 (= C99), C99 (= C102), C107 (= C110), C158 (= C152)
5z0cA Nerol dehydrogenase from persicaria minor (see paper)
36% identity, 98% coverage: 3:335/339 of query aligns to 4:345/367 of 5z0cA
- active site: C44 (= C41), S46 (= S43), H49 (≠ S46), H66 (= H63), C160 (= C152)
- binding zinc ion: C44 (= C41), H66 (= H63), C97 (= C96), C100 (= C99), C103 (= C102), C111 (≠ V115), C160 (= C152)
1uufA Crystal structure of a zinc-type alcohol dehydrogenase-like protein yahk
34% identity, 99% coverage: 1:335/339 of query aligns to 1:333/339 of 1uufA
- active site: C38 (= C41), H39 (= H42), S40 (= S43), H43 (≠ S46), H60 (= H63), E61 (= E64), C91 (= C96), C94 (= C99), C97 (= C102), C105 (≠ V108), T109 (≠ E112), C156 (= C152), T160 (= T156), R330 (= R332)
- binding zinc ion: C38 (= C41), H60 (= H63), C91 (= C96), C94 (= C99), C97 (= C102), C105 (≠ V108), C156 (= C152)
Sites not aligning to the query:
3twoB The crystal structure of cad from helicobacter pylori complexed with NADP(h) (see paper)
33% identity, 91% coverage: 28:335/339 of query aligns to 29:341/348 of 3twoB
- active site: C42 (= C41), H43 (= H42), S44 (= S43), H47 (≠ S46), H64 (= H63), E65 (= E64), C95 (= C96), C98 (= C99), C101 (= C102), C109 (vs. gap), V113 (vs. gap), C160 (= C152), T164 (= T156), R338 (= R332)
- binding nadph dihydro-nicotinamide-adenine-dinucleotide phosphate: C42 (= C41), H43 (= H42), S44 (= S43), H47 (≠ S46), C160 (= C152), T164 (= T156), G184 (= G176), F185 (≠ I177), G186 (= G178), G187 (= G179), L188 (= L180), R208 (≠ S200), T241 (= T238), I242 (≠ V239), P243 (≠ A240), T244 (≠ V241), V264 (= V261), G265 (= G262), L266 (≠ M265), L292 (≠ A286), I293 (≠ T287), L330 (≠ V324), G333 (= G327), R338 (= R332)
- binding zinc ion: C42 (= C41), H64 (= H63), C95 (= C96), C98 (= C99), C101 (= C102), C109 (vs. gap), C160 (= C152)
5fi3A Heteroyohimbine synthase thas1 from catharanthus roseus - complex with NADP+ (see paper)
34% identity, 97% coverage: 8:335/339 of query aligns to 10:336/344 of 5fi3A
- active site: C43 (= C41), Q44 (≠ H42), Y45 (≠ S43), E48 (≠ S46), H65 (= H63), E66 (= E64), C96 (= C96), C99 (= C99), C102 (= C102), C110 (= C110), G114 (= G113), C151 (= C152), R333 (= R332)
- binding nadp nicotinamide-adenine-dinucleotide phosphate: Q44 (≠ H42), E48 (≠ S46), T155 (= T156), G176 (= G176), L177 (≠ I177), G178 (= G178), G179 (= G179), L180 (= L180), S199 (= S199), S200 (= S200), K204 (= K204), T239 (= T238), I240 (≠ V239), P241 (≠ A240), L262 (≠ V261), G263 (= G262), T287 (≠ A286), S328 (≠ G327)
- binding zinc ion: C43 (= C41), H65 (= H63), E66 (= E64), C96 (= C96), C99 (= C99), C102 (= C102), C110 (= C110), C151 (= C152)
7cguA Crystal structure of abhar
34% identity, 98% coverage: 7:338/339 of query aligns to 7:346/352 of 7cguA
P12311 Alcohol dehydrogenase; ADH-T; EC 1.1.1.1 from Geobacillus stearothermophilus (Bacillus stearothermophilus) (see paper)
31% identity, 99% coverage: 4:338/339 of query aligns to 1:337/337 of P12311
- C38 (= C41) mutation to S: No activity.
- T40 (≠ S43) mutation to A: No activity.; mutation to S: Little decrease in activity.
- H43 (≠ S46) mutation to A: No activity.; mutation to R: Higher level of activity at pH 9.
6iqdA Crystal structure of alcohol dehydrogenase from geobacillus stearothermophilus (see paper)
30% identity, 98% coverage: 4:336/339 of query aligns to 1:335/336 of 6iqdA
- active site: C38 (= C41), T40 (≠ S43), H43 (≠ S46), H61 (= H63), C148 (= C152)
- binding zinc ion: C38 (= C41), H61 (= H63), E62 (= E64), C92 (= C96), C95 (= C99), C98 (= C102), C106 (= C110), C148 (= C152)
5h83A Heteroyohimbine synthase hys from catharanthus roseus - apo form (see paper)
34% identity, 97% coverage: 7:335/339 of query aligns to 14:345/354 of 5h83A
8a3nB Geissoschizine synthase from catharanthus roseus - binary complex with NADP+ (see paper)
33% identity, 91% coverage: 29:335/339 of query aligns to 32:343/352 of 8a3nB
- binding nadp nicotinamide-adenine-dinucleotide phosphate: N45 (≠ H42), V161 (≠ T156), G182 (= G176), L183 (≠ I177), L186 (= L180), S205 (= S199), T206 (≠ S200), K210 (= K204), T245 (= T238), P247 (vs. gap), V269 (= V261), A271 (= A263), S294 (≠ A286), L335 (≠ G327), R340 (= R332)
- binding zinc ion: C44 (= C41), H66 (= H63), E67 (= E64), C97 (= C96), C100 (= C99), C103 (= C102), C111 (= C110), C157 (= C152)
1rjwA Crystal structure of NAD(+)-dependent alcohol dehydrogenase from bacillus stearothermophilus strain lld-r (see paper)
31% identity, 98% coverage: 4:335/339 of query aligns to 1:334/339 of 1rjwA
- active site: C38 (= C41), H39 (= H42), T40 (≠ S43), H43 (≠ S46), H61 (= H63), E62 (= E64), C92 (= C96), C95 (= C99), C98 (= C102), C106 (= C110), K110 (≠ S114), C148 (= C152), T152 (= T156), R331 (= R332)
- binding trifluoroethanol: T40 (≠ S43), C148 (= C152), I285 (≠ A286)
- binding zinc ion: C38 (= C41), H61 (= H63), C92 (= C96), C95 (= C99), C98 (= C102), C106 (= C110)
8b1vA Dihydroprecondylocarpine acetate synthase 2 from tabernanthe iboga (see paper)
31% identity, 94% coverage: 16:335/339 of query aligns to 23:349/358 of 8b1vA
Query Sequence
>BWI76_RS03140 BWI76_RS03140 alcohol dehydrogenase
MSTIKSYAAKEAGADLSLWEYDAGDLKPEDVEVQVEYCGICHSDLSMIDNEWGMSSYPLV
AGHEVIGRVAALGSAAQDKGLKVGQKVGIGWTARSCGHCDACISGNHVNCLEGSVPTIIN
RGGFADKLRADWQWVIPLPENVDLESAGPMLCGGITVFKPLLMHHVTANSRVGVIGIGGL
GHIAIKLLHAMGAEVTAFSSNPAKEQEVLAMGADRVVNSRDPEALKALAGQFDLIINTVA
VDLDWQPYFEALAYGGNFHTVGAVMKPFPVPAFTLIGGDRSISGSATGNPAELRKLMKFA
GRSKVAPTTELFPMSQINEALKHVREGKARYRVVLKADF
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SitesBLAST's Database
SitesBLAST's database includes
(1) SwissProt
entries with experimentally-supported functional features;
and (2) protein structures with bound ligands, from the
BioLip database.
by Morgan Price,
Arkin group
Lawrence Berkeley National Laboratory