SitesBLAST

P80601 2-iminobutanoate/2-iminopropanoate deaminase; 14.3 kDa perchloric acid soluble protein; Translation inhibitor L-PSP ribonuclease; UK114 antigen; EC 3.5.99.10; EC 3.1.-.- from Capra hircus (Goat) (see paper)
34% identity, 78% coverage: 20:121/130 of query aligns to 20:124/137 of P80601

query
sites
P80601

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Sites not aligning to the query:

1 modified: Initiator methionine, Removed
2 modified: N-acetylserine

A0A1J1DL12 2-iminobutanoate/2-iminopropanoate deaminase; Allergen Der f 34; Enamine/imine deaminase; Allergen Der f 34.0101; EC 3.5.99.10 from Dermatophagoides farinae (American house dust mite) (see paper)
32% identity, 78% coverage: 20:121/130 of query aligns to 18:122/128 of A0A1J1DL12

query
sites
A0A1J1DL12

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Y19 (≠ F21) mutation to A: Loss of imine deaminase activity; when associated with A-33; A-59; A-91; A-105; A-114 and A-120.
G33 (= G35) mutation to A: Loss of imine deaminase activity; when associated with A-19; A-59; A-91; A-105; A-114 and A-120.
N59 (= N59) mutation to A: Loss of imine deaminase activity; when associated with A-19; A-33; A-91; A-105; A-114 and A-120.
N91 (= N91) mutation to A: Loss of imine deaminase activity; when associated with A-19; A-33; A-59; A-105; A-114 and A-120.
R105 (= R105) mutation to A: Loss of imine deaminase activity; when associated with A-19; A-33; A-59; A-91; A-114 and A-120.
P114 (≠ V113) mutation to A: Loss of imine deaminase activity; when associated with A-19; A-33; A-59; A-91; A-105 and A-120.
E120 (= E119) mutation to A: Loss of imine deaminase activity; when associated with A-19; A-33; A-59; A-91; A-105 and A-114.

Sites not aligning to the query:

1 modified: Initiator methionine, Removed

3k0tC Crystal structure of pspto -psp protein in complex with d-beta-glucose from pseudomonas syringae pv. Tomato str. Dc3000 (see paper)
34% identity, 78% coverage: 20:121/130 of query aligns to 15:118/124 of 3k0tC

query
sites
3k0tC

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binding beta-D-glucopyranose: Y16 (≠ F21), G30 (= G35), I32 (≠ T37), F83 (= F87), R101 (= R105), R111 (≠ V114), E116 (= E119)

Q7CP78 2-iminobutanoate/2-iminopropanoate deaminase; Enamine/imine deaminase; EC 3.5.99.10 from Salmonella typhimurium (strain LT2 / SGSC1412 / ATCC 700720) (see paper)
32% identity, 78% coverage: 20:121/130 of query aligns to 16:122/128 of Q7CP78

query
sites
Q7CP78

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Y17 (≠ F21) mutation to F: Deaminase activity is defective. At pH 8, strongly reduces the increase in rate of 2-ketobutyrate formation over the rate of IlvA alone. At pH 9.5, moderately reduces the increase in rate of 2-ketobutyrate formation over the rate of IlvA alone.
R105 (= R105) mutation to A: Deaminase activity is defective. At pH 8 and 9.5, strongly reduces the increase in rate of 2-ketobutyrate formation over the rate of IlvA alone.
E120 (= E119) Important for catalytic activity at high pH; mutation to A: Deaminase activity is defective. At pH 8, moderately reduces the increase in rate of 2-ketobutyrate formation over the rate of IlvA alone. At pH 9.5, strongly reduces the increase in rate of 2-ketobutyrate formation over the rate of IlvA alone.; mutation to K: Deaminase activity is defective. At pH 8, moderately reduces the increase in rate of 2-ketobutyrate formation over the rate of IlvA alone. At pH 9.5, strongly reduces the increase in rate of 2-ketobutyrate formation over the rate of IlvA alone.

3vczB 1.80 angstrom resolution crystal structure of a putative translation initiation inhibitor from vibrio vulnificus cmcp6
30% identity, 78% coverage: 20:121/130 of query aligns to 15:122/127 of 3vczB

query
sites
3vczB

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binding magnesium ion: D79 (= D83), N81 (≠ R85)

P0AFQ5 3-aminoacrylate deaminase RutC; 3-AA deaminase; EC 3.5.-.- from Escherichia coli (strain K12) (see paper)
28% identity, 83% coverage: 13:120/130 of query aligns to 10:120/128 of P0AFQ5

query
sites
P0AFQ5

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R104 (= R105) mutation to A: Loss of activity.

5v4dA Crystal structure of the protein of unknown function of the conserved rid protein family yyfa from yersinia pestis
27% identity, 61% coverage: 20:98/130 of query aligns to 17:97/127 of 5v4dA

query
sites
5v4dA

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binding calcium ion: D91 (≠ K92), D95 (≠ E96)

O30478 3-hydroxybenzoate synthase; EC 4.1.3.45 from Streptomyces hygroscopicus (see paper)
28% identity, 88% coverage: 4:117/130 of query aligns to 209:336/340 of O30478

query
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O30478

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R220 (≠ G15) binding
G240 (= G35) Important for product selectivity; mutation to A: Decrease of the 3-hydroxybenzoate synthase activity compared to the wild-type and complete loss of product selectivity.
C327 (= C108) Important for product selectivity; mutation to A: Decrease of the 3-hydroxybenzoate synthase activity compared to the wild-type and complete loss of product selectivity.
E334 (≠ D115) active site, Proton acceptor; mutation to Q: Loss of chorismatase activity.

Sites not aligning to the query:

147 binding
154 binding
207 binding

5ag3A Chorismatase mechanisms reveal fundamentally different types of reaction in a single conserved protein fold (see paper)
28% identity, 88% coverage: 4:117/130 of query aligns to 202:329/333 of 5ag3A

query
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5ag3A

K

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binding 3-(2-carboxyethyl)benzoic acid: F211 (≠ G13), G233 (= G35), C320 (= C108)

Sites not aligning to the query:

binding 3-(2-carboxyethyl)benzoic acid: 140, 147, 164, 165, 166, 200

Query Sequence

>BWI76_RS03895 FitnessBrowser__Koxy:BWI76_RS03895
MSIKRYGVEGGTGTGGQHLPFARAVEAGGWLYVSGQTPMKDGEVVEGGIVEQSRLAIQNC
VDIMNEAGYSLADVVHVKVILTDSRYFQSFNKVFREFFADNPPARICCVADLVVDCKVEV
DVTCYNAARV

Or try a new SitesBLAST search

SitesBLAST's Database

SitesBLAST's database includes (1) SwissProt entries with experimentally-supported functional features; and (2) protein structures with bound ligands, from the BioLip database.

by Morgan Price, Arkin group
Lawrence Berkeley National Laboratory