SitesBLAST

SitesBLAST – Find functional sites

SitesBLAST

Comparing BWI76_RS04430 FitnessBrowser__Koxy:BWI76_RS04430 to proteins with known functional sites using BLASTp with E ≤ 0.001.

Or try Sites on a Tree, PaperBLAST, Conserved Domains, or compare to all protein structures

Found 4 hits to proteins with known functional sites (download)

5a1sD Crystal structure of the sodium-dependent citrate symporter secits form salmonella enterica. (see paper)
93% identity, 97% coverage: 16:446/446 of query aligns to 2:432/434 of 5a1sD

query
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binding citrate anion: L323 (= L337), G389 (= G403), G390 (= G404), S391 (= S405)
binding sodium ion: I167 (= I181), M168 (= M182), G169 (= G183), C384 (= C398), M385 (= M399), M385 (= M399), A386 (= A400), N387 (= N401), N387 (= N401), G389 (= G403), D393 (= D407), S413 (= S427)

5x9rA Structural insights into the elevator-like mechanism of the sodium/citrate symporter cits (see paper)
93% identity, 95% coverage: 21:445/446 of query aligns to 1:416/416 of 5x9rA

query
sites
5x9rA

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binding beta-D-glucopyranose: A108 (= A128), V112 (= V132), E225 (= E245), N389 (= N418), Y393 (= Y422)
binding citrate anion: G165 (= G185), N166 (= N186), Y319 (= Y348), R373 (= R402), G375 (= G404), S376 (= S405), R399 (= R428)

5xarD Structural insights into the elevator-like mechanism of the sodium/citrate symporter cits (see paper)
90% identity, 97% coverage: 13:446/446 of query aligns to 1:411/411 of 5xarD

query
sites
5xarD

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L

L

I

I

S

S

Y

Y

A

A

Q

Q

I

I

S

S

S

S

R

R

L

L

G

G

G

G

G

G

I

I

V

V

L

L

V

V

I

I

A

A

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S

I

I

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V

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F

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M

M

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M

I

binding sodium ion: I169 (= I181), M170 (= M182), G171 (= G183), L204 (= L216), M364 (= M399), A365 (= A400), N366 (= N401)

5xasB Structural insights into the elevator-like mechanism of the sodium/citrate symporter cits (see paper)
90% identity, 97% coverage: 16:446/446 of query aligns to 1:407/407 of 5xasB

query
sites
5xasB

S

S

D

D

I

L

L

L

G

G

F

F

K

K

I

I

F

F

G

G

M

M

P

P

L

L

P

P

L

L

Y

Y

A

A

F

F

A

A

L

L

I

I

T

T

L

L

L

L

L

L

S

S

H

H

F

F

Y

Y

N

N

A

A

L

L

P

P

T

T

D

D

I

I

V

V

G

G

G

G

F

F

A

A

I

I

M

M

F

F

I

I

I

I

G

G

A

A

V

I

F

F

G

G

E

E

I

I

G

G

K

K

R

R

L

L

P

P

I

I

F

F

N

N

K

K

Y

Y

I

I

G

G

G

G

A

A

P

P

V

V

M

M

I

I

F

F

L

L

V

V

A

A

A

A

Y

Y

F

F

V

V

Y

Y

A

A

G

G

I

I

F

F

T

T

Q

Q

K

K

E

E

I

I

E

D

A

A

I

I

T

S

N

N

V

V

M

M

D

D

K

K

S

S

N

N

F

F

L

L

N

N

L

L

F

F

I

I

A

A

V

V

L

L

I

I

T

T

G

G

A

A

I

I

L

L

S

S

V

V

N

N

R

R

K

R

L

L

L

L

L

L

K

K

S

S

L

L

L

L

G

G

Y

Y

I

I

P

P

T

T

I

I

L

L

M

M

G

G

I

I

L

V

G

G

A

A

S

S

I

I

F

F

G

G

I

I

L

A

I

I

G

G

L

L

C

V

F

F

G

G

I

I

S

P

V

V

D

D

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I

I

M

M

M

M

L

L

Y

Y

V

V

L

L

P

P

I
|
I

M
|
M

G
|
G

G

G

G

G

N

N

G

G

A

A

G

G

A

A

V

V

P

P

L

L

S

S

E

E

I

I

Y

Y

H

H

S

S

V

V

T

T

G

G

R

R

S

S

R

R

E

E

E

E

Y

Y

Y

Y

S

S

T

T

A

A

I

I

A

A

I

I

L

L

T

T

I

I

A

A

N

N

I

I

F

F

A

A

I

I

V

V

F

F

A

A

A

A

L

V

L

L

D

D

I

I

I

I

G

G

K

K

K

K

H

H

T

T

W

-

L

-

S

-

G

-

E

-

G

-

E

-

L

-

V

-

R

-

K

-

A

-

S

-

F

-

K

-

V

-

E

-

D

-

D

-

E

-

K

-

A

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G

-

Q

-

I

I

T

T

H

H

R

R

E

E

T

T

A

A

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V

G

G

L

L

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V

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L

A

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T

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T

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C

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F

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A

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Y

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A

A

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K

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I

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L

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P

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S

I

I

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G

G

G

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V

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A

I

I

H

H

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Y

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F

A

A

W

W

M

M

V

V

L

L

I

I

V

V

A

A

A

A

L

L

N

N

A

A

S

S

G

G

L

L

C

C

S

S

P

P

E

E

I

I

K

K

A

A

G

G

A

A

K

K

R

R

L

L

S

S

D

D

F

F

F

F

S

S

K

K

Q

Q

L

L

L
|
L

W

W

V

V

L

L

M

M

V

V

G

G

V

V

G

G

V

V

C

C

Y

Y

T

T

D

D

L

L

Q

Q

E

E

I

I

I

I

D

N

A

A

I

I

T

T

F

F

A

A

N

N

V

V

V

V

I

I

A

A

A

A

V

I

I

I

V

V

V

I

G

G

A

A

V

V

V

L

G

G

A

A

A

A

V

I

G

G

G

G

W

W

L

L

I

M

G

G

F

F

F

F

P

P

I

I

E

E

S

S

A

A

I

I

T

T

A

A

G

G

L

L

C

C

M
|
M

A
|
A

N
|
N

R

R

G

G

G
|
G

S
|
S

G

G

D

D

L

L

E

E

V

V

L

L

S

S

A

A

C

C

N

N

R

R

M

M

N

N

L

L

I

I

S

S

Y

Y

A

A

Q

Q

I

I

S

S

S

S

R

R

L

L

G

G

G

G

G

G

I

I

V

V

L

L

V

V

I

I

A

A

S

S

I

I

V

V

F

F

G

G

M

M

M

M

M

I

binding citrate anion: L298 (= L337), G365 (= G404), S366 (= S405)
binding sodium ion: I166 (= I181), M167 (= M182), G168 (= G183), M360 (= M399), A361 (= A400), N362 (= N401)

Query Sequence

>BWI76_RS04430 FitnessBrowser__Koxy:BWI76_RS04430
MTNMSQAPSAEKKGVSDILGFKIFGMPLPLYAFALITLLLSHFYNALPTDIVGGFAIMFI
IGAVFGEIGKRLPIFNKYIGGAPVMIFLVAAYFVYAGIFTQKEIEAITNVMDKSNFLNLF
IAVLITGAILSVNRKLLLKSLLGYIPTILMGILGASIFGILIGLCFGISVDRIMMLYVLP
IMGGGNGAGAVPLSEIYHSVTGRSREEYYSTAIAILTIANIFAIVFAALLDIIGKKHTWL
SGEGELVRKASFKVEDDEKAGQITHRETAVGLVLATTCFLLAYVIAKKILPSIGGVSIHY
FAWMVLIVAALNASGLCSPEIKAGAKRLSDFFSKQLLWVLMVGVGVCYTDLQEIIDAITF
ANVVIAAVIVVGAVVGAAVGGWLIGFFPIESAITAGLCMANRGGSGDLEVLSACNRMNLI
SYAQISSRLGGGIVLVIASIVFGMMM

Or try a new SitesBLAST search

SitesBLAST's Database

SitesBLAST's database includes (1) SwissProt entries with experimentally-supported functional features; and (2) protein structures with bound ligands, from the BioLip database.

by Morgan Price, Arkin group
Lawrence Berkeley National Laboratory