SitesBLAST

SitesBLAST – Find functional sites

SitesBLAST

Comparing BWI76_RS04950 FitnessBrowser__Koxy:BWI76_RS04950 to proteins with known functional sites using BLASTp with E ≤ 0.001.

Or try Sites on a Tree, PaperBLAST, Conserved Domains, or compare to all protein structures

Found 20 (the maximum) hits to proteins with known functional sites (download)

1kv9A Structure at 1.9 a resolution of a quinohemoprotein alcohol dehydrogenase from pseudomonas putida hk5 (see paper)
28% identity, 77% coverage: 161:773/796 of query aligns to 11:521/664 of 1kv9A

query
sites
1kv9A

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active site: E173 (≠ D354), N250 (≠ V422), D295 (= D466)
binding acetone: E173 (≠ D354), D295 (= D466), P377 (≠ N586), F378 (≠ L587)
binding calcium ion: E173 (≠ D354), N250 (≠ V422), D295 (= D466)
binding heme c: A101 (≠ F260), R102 (≠ Q261)
binding pyrroloquinoline quinone: E59 (= E217), C105 (≠ T264), C106 (= C265), R111 (≠ Y270), T155 (= T336), G170 (≠ A351), G171 (≠ V352), A172 (≠ T353), E173 (≠ D354), T230 (≠ S403), W232 (= W404), N250 (≠ V422), D295 (= D466), K322 (= K493), N382 (≠ E591), W383 (= W592), W460 (≠ V701)

Sites not aligning to the query:

binding heme c: 590, 591, 594, 595, 605, 606, 608, 611, 615, 619, 622, 623, 631, 633, 634, 636
binding pyrroloquinoline quinone: 524, 525

Q8GR64 Quinohemoprotein alcohol dehydrogenase ADH IIB; ADH IIB; Alcohol dehydrogenase (azurin); EC 1.1.9.1 from Pseudomonas putida (Arthrobacter siderocapsulatus) (see 3 papers)
28% identity, 77% coverage: 161:773/796 of query aligns to 33:543/690 of Q8GR64

query
sites
Q8GR64

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S

N
x
P

L
x
F

G
|
G

M
x
A

F
x
H

E
x
N

W
|
W

G
x
H

G
x
S

I
x
M

S
|
S

V
x
F

D
x
N

P
|
P

N
x
G

R
x
T

Q
x
G

V
x
L

A
x
V

I
x
Y

A
x
I

N
x
P

P
x
Y

M
x
Q

A
x
E

L
x
V

P
|
P

F
x
G

V
|
V

S
x
Y

K
x
R

L

-

I

-

P

-

R

-

G

-

P

-

G

-

N
|
N

P
x
E

M
x
G

E
x
K

Q
x
D

P
x
F

K
x
V

D
x
T

A
x
R

K
|
K

G
x
A

S
x
F

G
x
N

T
|
T

E
x
A

A
|
A

G
|
G

I
x
F

Q
x
A

P
x
D

Q
x
A

Y
x
T

G
x
D

V
|
V

P
|
P

Y
x
A

G
x
A

V
|
V

T
x
V

L
x
S

N
x
G

P
x
A

F
x
L

L
|
L

S
x
A

P

-

F

-

G
x
W

L
x
D

P
|
P

C
x
V

K
|
K

Q
|
Q

P
x
K

A
|
A

W

-

G

-

Y

-

I

-

S

-

A

-

L

-

D

-

L

-

K

-

T

-

N

-

E

-

V

-

V
x
A

W
|
W

K
|
K

K

-

R

-

I

-

G

-

T

-

P

-

Q

-

D

-

S

-

L
x
V

P
|
P

F
x
Y

P
|
P

M
x
T

P
x
H

V
x
W

K
x
N

L

-

P

-

F

-

T

-

M

-

G

-

M

-

P

-

M

-

L

-

G
|
G

G
|
G

P
x
T

I
x
L

S
|
S

T
|
T

A
|
A

G
|
G

N
|
N

V
x
L

L
x
V

F
|
F

I
x
Q

G
|
G

A

-

T
|
T

A
|
A

D
x
A

N
x
G

Y
x
Q

L
x
M

R
x
H

A
|
A

Y
|
Y

N
x
S

M
x
A

S
x
D

N
x
K

G
|
G

E
|
E

K
x
A

L
|
L

W
|
W

Q
|
Q

A
x
F

R
x
E

L
x
A

P
x
Q

A
x
S

G
|
G

G
x
I

Q
x
V

A
|
A

T
x
A

P
|
P

M
|
M

T
|
T

Y
x
F

E
|
E

V
x
L

N
x
A

G
|
G

K
x
R

Q
|
Q

Y
|
Y

V
|
V

V
x
A

I
|
I

S
x
M

A
|
A

G
|
G

E81 (= E217) binding
C127 (≠ T264) modified: Disulfide link with 128
C128 (= C265) modified: Disulfide link with 127
R133 (≠ Y270) binding
T177 (= T336) binding
GA 193:194 (≠ VT 352:353) binding
E195 (≠ D354) binding
T252 (≠ S403) binding
N272 (≠ V422) binding
D317 (= D466) binding
K344 (= K493) binding
NW 404:405 (≠ EW 591:592) binding

Sites not aligning to the query:

1:22 signal peptide
23:690 modified: mature protein, Quinohemoprotein alcohol dehydrogenase ADH IIB
547 binding
613 binding covalent
616 binding covalent
617 binding axial binding residue
655 binding axial binding residue

Q4W6G0 Quinohemoprotein alcohol dehydrogenase ADH-IIG; ADH IIG; Alcohol dehydrogenase (azurin); EC 1.1.9.1 from Pseudomonas putida (Arthrobacter siderocapsulatus) (see 2 papers)
28% identity, 83% coverage: 124:783/796 of query aligns to 14:577/718 of Q4W6G0

query
sites
Q4W6G0

L
|
L

V
x
A

V
|
V

A
|
A

L
x
V

L
|
L

I
x
L

S
|
S

G
x
L

G
x
A

I
x
G

L
x
T

T
x
P

W
x
A

A
x
L

G
x
A

F
x
A

N
x
D

D
x
I

P
|
P

Q
x
A

E
x
N

V
|
V

N
x
D

G
|
G

T
x
A

-
x
R

-
x
I

L
x
I

S
x
A

A
|
A

D
|
D

A
x
K

T
x
E

P
|
P

A

-

A

-

P

-

I

-

S

-

E

-

V

-

A

-

D

-

G
|
G

D
x
N

W
|
W

S
x
M

A
x
S

Y
x
T

G
|
G

R
|
R

N
x
T

Q
x
Y

E
x
D

G
x
E

Q
|
Q

R
|
R

Y
|
Y

S
|
S

P
|
P

L
|
L

K
|
K

Q
|
Q

I
|
I

N
x
S

A
x
D

D
x
Q

N
|
N

V
|
V

K
x
G

N
x
Q

L
|
L

K
x
G

E
x
L

A
|
A

W
|
W

V
x
S

F
x
Y

R
x
K

T
x
L

G

-

D
|
D

L
|
L

K
x
D

Q
x
R

P

-

N

-

D

-

P

-

G

-

E

-

I

-

T
x
G

N
x
V

E
|
E

V
x
A

T
|
T

P
|
P

I
|
I

K
x
V

V
|
V

G
x
D

D
x
G

T
x
A

L
x
M

Y
|
Y

L
x
T

C
x
T

T
x
G

A
x
P

H
x
F

Q
x
S

R
x
V

L
x
V

F
x
Y

A
|
A

L
|
L

D
|
D

A
|
A

A
x
R

T
x
D

G
|
G

K
x
R

E
x
L

K
x
I

W
|
W

H
x
K

F
x
Y

D
|
D

P
|
P

Q
|
Q

L
x
S

N
x
D

A
x
R

D
x
H

P
x
R

S
x
A

F
x
G

Q
x
E

H

-

V
x
A

T
x
C

C
|
C

R
x
D

G
x
A

V
|
V

S
x
N

Y
x
R

H
x
G

E

-

A

-

K

-

A

-

D

-

N

-

A

-

P

-

A

-

D

-

V

-

V
|
V

A
|
A

D
x
V

C
x
W

P
x
K

R
x
G

R
x
K

I
x
V

I
x
Y

L
x
V

P
x
G

V
|
V

N
x
L

D
|
D

G
|
G

R
|
R

L
|
L

F
x
E

A
|
A

I
|
I

N
x
D

A
|
A

E
x
K

T
|
T

G
|
G

K
x
Q

L
x
R

C
x
A

E
x
W

S
|
S

F
x
V

A
x
D

N
x
T

K
x
R

G
x
A

I
x
D

L
x
H

N
x
K

L
x
R

Q
x
S

T

-

N

-

M

-

P

-

V

-

T

-

T

-

P

-

G

-

M

-

Y
|
Y

E
x
T

P
x
I

T
|
T

S
x
G

P
x
A

P
|
P

I
x
R

I
x
V

T
x
V

D
x
N

K
x
G

T
x
K

I
x
V

V
|
V

I
|
I

A
x
G

G
x
N

A
x
G

V
x
G

T
x
A

D
x
E

N

-

F
|
F

S
x
G

T
x
V

R
|
R

E

-

P

-

S

-

G
|
G

V
x
Y

I
x
V

R
x
T

G
x
A

F
x
Y

D
|
D

V
x
A

N
x
E

T
|
T

G
|
G

K
|
K

L
x
E

L
x
A

W
|
W

A
x
R

F
|
F

D
x
Y

P
x
T

G
x
V

A
x
P

K
x
G

D
|
D

P
|
P

N
x
K

A
x
L

I
x
P

P
|
P

D
x
E

-
x
G

-
x
K

-
x
G

-
x
M

-
x
E

-
x
I

-
x
A

-
x
A

-
x
K

-
x
T

-
x
W

-
x
F

-
x
G

D
|
D

E
x
A

H
x
Y

H
x
V

F
x
E

T
x
Q

L
x
G

N
x
G

S
x
G

P
x
G

N
x
T

S
x
A

W
|
W

A
x
D

P
x
S

A
x
F

A
|
A

Y
|
Y

D
|
D

A
x
P

K
x
E

L
|
L

D
x
N

L
|
L

V
x
L

Y
|
Y

L
x
I

P

-

M

-

G
|
G

V
|
V

T
x
G

T
x
N

P
x
G

D
x
S

I
x
L

W
|
W

G
x
D

-
x
P

-
x
K

-
x
W

G
x
R

N
x
S

R
x
Q

T
x
A

P
x
K

E
x
G

Q
x
D

E
x
N

R
x
L

Y
x
F

A
x
L

S
|
S

S
|
S

I
|
I

V
|
V

A
|
A

L
x
V

N
|
N

A
|
A

T
x
D

T
|
T

G
|
G

K
x
E

L
x
Y

A
x
V

W
|
W

S
x
H

Y
|
Y

Q
|
Q

T
|
T

V
x
T

H
x
P

H
x
G

D
|
D

L
x
A

W
|
W

D
|
D

M
x
Y

D
x
T

M
x
A

P
x
T

S
x
Q

Q
x
H

P
x
M

T
x
I

L
|
L

A
|
A

D
x
E

I
x
L

D
x
P

V
x
I

N
x
D

G
|
G

K
|
K

T
x
P

V
x
R

P
x
K

V
|
V

I
x
L

Y
x
M

A
x
Q

P
x
A

A
x
P

K
|
K

T
x
N

G
|
G

N
x
F

I
x
F

F
x
Y

V
|
V

L
x
I

D
|
D

R
|
R

R
x
A

N
x
T

G
|
G

E
|
E

L
|
L

V
x
L

V

-

P

-

A
x
S

P
x
A

E
x
K

K
x
G

P
x
I

V
|
V

P
|
P

Q
|
Q

G
x
S

A
x
W

A
x
T

K
|
K

G
|
G

D

-

Y

-

V

-

A

-

K

-

T

-

Q

-

P

-

F
x
M

S
x
D

D
x
M

L
x
K

S
x
T

F
x
G

R
|
R

P
|
P

K
x
I

K
x
L

D
|
D

L
x
E

T
x
E

G
x
N

A
|
A

D
x
A

M
x
Y

W
|
W

G

-

A

-

T

-

M

-

F

-

D

-

Q

-

L

-

V

-

C

-

R

-

V

-

I

-

F

-

H

-

Q

-

L

-

R

-

Y

-

E

-

G

-

I

-

F

-

T

-

P

-

P
x
K

S
x
N

E
x
G

Q
x
K

G
x
R

T
x
N

L
|
L

V
|
V

F
x
T

P
|
P

G
x
A

N
x
F

L
x
W

G
|
G

M
x
A

F
x
H

E
x
D

W
|
W

G
x
Q

G
x
P

I
x
M

S
|
S

V
x
Y

D
x
N

P
|
P

N
x
D

R
x
T

Q
x
G

V
x
L

A
x
V

I
x
Y

A
x
I

N
x
P

P
x
A

M
x
H

A
x
I

L
x
M

P
x
S

F
x
A

V
x
Y

S
x
Y

K
x
E

L
x
H

I
|
I

P
|
P

R
x
E

G
x
A

P
|
P

G
x
K

-
x
R

N
|
N

P
|
P

M
x
F

E

-

Q

-

P

-

K
|
K

D
x
S

A
x
M

K
x
Y

G
x
Q

S
x
L

G
|
G

T
x
L

E
x
R

A
x
T

G
|
G

I
x
M

Q
x
M

P
|
P

Q
x
E

Y

-

G

-

V

-

P

-

Y

-

G

-

V

-

T

-

L

-

N

-

P

-

F

-

L

-

S
x
G

P
x
A

F
x
E

G
|
G

L
|
L

P
x
L

C
x
E

K
x
M

Q
x
A

P
x
K

A
x
S

W
|
W

-
x
S

G
|
G

Y
x
K

I
x
L

S
x
I

A
|
A

L
x
W

D
|
D

L
x
P

K
x
V

T
x
K

N
x
Q

E
x
Q

V
x
A

V
x
A

W
|
W

K
x
E

K

-

R

-

I

-

G

-

T

-

P

-

Q

-

D

-

S

-

L

-

P

-

F

-

P

-

M
x
V

P
|
P

V
x
Y

K
x
V

L
x
T

P
x
I

F
|
F

T
x
N

M

-

G

-

M

-

P

-

M

-

L

-

G
|
G

G
|
G

P
x
T

I
x
L

S
|
S

T
|
T

A
|
A

G
|
G

N
|
N

V
x
L

L
x
V

F
|
F

I
x
E

G
|
G

A

-

T
x
S

A
|
A

D
|
D

N
x
G

Y
x
R

L
x
V

R
x
I

A
|
A

Y
|
Y

N
x
A

M
x
A

S
x
D

N
x
T

G
|
G

E
|
E

K
|
K

L
|
L

W
|
W

Q
x
E

A
x
Q

R
x
P

L
x
A

P
x
A

A
x
S

G
|
G

G
x
V

Q
x
M

A
|
A

T
x
A

P
|
P

M
x
V

T
|
T

Y
|
Y

E
x
S

V
|
V

N
x
D

G
|
G

K
x
E

Q
|
Q

Y
|
Y

V
|
V

V
x
T

I
x
F

S
x
M

A
|
A

G
|
G

G
x
W

H
x
G

G
|
G

S
x
A

F
|
F

G
x
S

T
|
T

K
x
F

M
x
A

G
|
G

C138 (≠ T264) modified: Disulfide link with 139
C139 (= C265) modified: Disulfide link with 138
R144 (≠ Y270) binding
T189 (= T336) binding
GA 205:206 (≠ VT 352:353) binding
E207 (≠ D354) binding
T264 (≠ N402) binding
N284 (≠ T424) binding
D329 (= D466) binding
K356 (= K493) binding
W415 (≠ L587) binding
DW 419:420 (≠ EW 591:592) binding

Sites not aligning to the query:

1:29 signal peptide
30:718 modified: mature protein, Quinohemoprotein alcohol dehydrogenase ADH-IIG
635 binding covalent
638 binding covalent
639 binding axial binding residue
676 binding axial binding residue

1yiqA Molecular cloning and structural analysis of quinohemoprotein alcohol dehydrogenase adhiig from pseudomonas putida hk5. Compariison to the other quinohemoprotein alcohol dehydrogenase adhiib found in the same microorganism. (see paper)
28% identity, 81% coverage: 143:783/796 of query aligns to 4:548/684 of 1yiqA

query
sites
1yiqA

P

P

Q

A

E

N

V

V

N

D

G

G

T

A

-

R

-

I

L

I

S

A

A

A

D

D

A

K

T

E

P

P

A

-

A

-

P

-

I

-

S

-

E

-

V

-

A

-

D

-

G

G

D

N

W

W

S

M

A

S

Y

T

G

G

R

R

N

T

Q

Y

E

D

G

E

Q

Q

R

R

Y

Y

S

S

P

P

L

L

K

K

Q

Q

I

I

N

S

A

D

D

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N

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V

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N

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L

L

K

G

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L

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W

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D

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|
E

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x
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x
C

C
|
C

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A

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|
T

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N

K

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V

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x
G

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x
G

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|
W

A

D

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A

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Y

Y

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L

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N

L

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L

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M

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G

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x
N

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|
D

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Y

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V

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P

K

V

V

I

L

Y

M

A

Q

P

A

A

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K
|
K

T

N

G

G

N

F

I

F

F

Y

V

V

L

I

D

D

R

R

R

A

N

T

G

G

E

E

L

L

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L

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-

P

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W

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|
W

G

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G

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F
|
F

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N

M

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G

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M

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P

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M

-

L

-

G

G

G

G

P

T

I

L

S

S

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A

A

G

G

N

N

V

L

L

V

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F

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G

G

A

-

T

S

A

A

D

D

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Y

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L

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A

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G

E

E

K

K

L

L

W

W

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Q

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P

L

A

P

A

A

S

G

G

G

V

Q

M

A

A

T

A

P

P

M

V

T

T

Y

Y

E

S

V

V

N

D

G

G

K

E

Q

Q

Y

Y

V

V

V

T

I

F

S

M

A

A

G

G

G

W

H

G

G
|
G

S
x
A

F

F

G

S

T

T

K

F

M

A

G

G

active site: E178 (≠ D354), N255 (≠ T424), D300 (= D466)
binding calcium ion: E178 (≠ D354), N255 (≠ T424), D300 (= D466)
binding heme c: R60 (≠ Q207), G106 (≠ F260)
binding pyrroloquinoline quinone: E63 (= E217), C109 (≠ T264), C110 (= C265), R115 (≠ Y270), T160 (= T336), G175 (≠ A351), G176 (≠ V352), A177 (≠ T353), E178 (≠ D354), T235 (≠ N402), W237 (= W404), N255 (≠ T424), D300 (= D466), K327 (= K493), W386 (≠ L587), D390 (≠ E591), W391 (= W592), F477 (= F705), G541 (= G776), A542 (≠ S777)

Sites not aligning to the query:

binding heme c: 605, 606, 608, 609, 610, 621, 623, 626, 630, 634, 637, 638, 642, 645, 646, 647, 648, 650, 654, 662

4aahA Methanol dehydrogenase from methylophilus w3a1 (see paper)
27% identity, 74% coverage: 178:768/796 of query aligns to 24:522/571 of 4aahA

query
sites
4aahA

Q

Q

R

H

Y

N

S

S

P

P

L

L

K

A

Q

Q

I

I

N

N

A

K

D

S

N

N

V

V

K

K

N

N

L

V

K

K

E

A

A

A

W

W

V

S

F

F

R

S

T

T

G

G

D

V

L

L

K

N

Q

-

P

-

N

-

D

-

P

-

G

-

E

-

I

-

T

G

N

H

E
|
E

V

G

T

A

P

P

I

L

K

V

V

I

G

G

D

D

T

M

L

M

Y

Y

L

V

C

H

T

S

A

A

H

F

-

P

Q

N

R

N

L

T

F

Y

A

A

L

L

D

N

A

L

A

N

T

D

-

P

G

G

K

K

E

I

K

V

W

W

H

Q

F

H

D

K

P

P

Q

K

L

-

N

-

A

Q

D

D

P

A

S

S

F

T

Q

K

H

A

V

V

T

M

C
|
C

-
x
C

-

D

-

V

-
x
V

-

D

R
|
R

G

G

V

L

S

A

Y

Y

H

G

E

A

A

G

K

-

A

-

D

-

N

-

A

-

P

-

A

-

D

-

V

-

V

-

A

-

D

-

C

-

P

-

R

-

R

Q

I

I

I

V

L

K

P

K

V

Q

N

A

D

N

G

G

R

H

L

L

F

L

A

A

I

L

N

D

A

A

E

K

T

T

G

G

K

K

L

I

C

-

E

-

S

-

F

-

A

-

N

-

K

-

G

-

I

-

L

-

N

-

L

-

Q

N

T

W

N

E

M

V

P

E

V

V

T

C

T

D

P

P

G

K

M

V

Y

G

E

S

P

T

T

L

S
x
T

P

Q

P

A

I

P

I

F

T

V

D

A

K

K

T

D

I

T

V

V

I

L

A

M

G

G

A

C

V
x
S

T
x
G

D
x
A

N
x
E

F

L

S

G

T

V

R

R

E

-

P

-

S

-

G

G

V

A

I

V

R

N

G

A

F

F

D

D

V

L

N

K

T

T

G

G

K

E

L

L

L

K

W

W

-

R

A

A

F

F

D

A

P

T

G

G

-

S

-

D

-

D

-

S

-

V

-

R

-

L

A

A

K

K

D

D

P

F

N

N

A

S

I

A

P

N

D

P

D

H

E

Y

H

G

H

Q

F

F

T

G

L

L

N

G

S

T

P

K

N

T

-

W

-

E

-

G

-

D

-

A

-

W

-

K

-

I

-

G

-

G

-

G

-
x
T

S

N

W
|
W

A

G

P

W

A

Y

A

A

Y

Y

D

D

A

P

K

K

L

L

D

N

L

L

V

F

Y

Y

L

Y

P

G

M

S

G

G

V
x
N

T

P

T

A

P

P

D

-

I

-

W

W

G

N

G

E

N

T

R

M

T

R

P

P

E

G

Q

D

E

N

R

K

Y

W

A

T

S

M

S

T

I

I

V

W

A

G

L

R

N

D

A

L

T

D

T

T

G

G

K

M

L

A

A

K

W

W

S

G

Y

Y

Q

Q

T

K

V

T

H

P

H

H

D

D

L

E

W

W

D
|
D

M

F

D

A

M

G

P

V

S

N

Q

Q

P

M

T

V

L

L

A

T

D

D

I

Q

D

P

V

V

N

N

G

G

K

K

T

M

V

T

P

P

V

L

I

L

Y

S

A

H

P

I

A

D

K
x
R

T

N

G

G

N

I

I

L

F

Y

V

T

L

L

D

N

R

R

R

E

N

N

G

G

E

N

L

L

V

I

V

V

P

A

A

E

P

K

E

V

K

D

P

P

V

A

P

V

Q

N

G

V

A

F

A

K

K

K

G

V

D

D

Y

L

V

K

A

T

K

G

T

T

Q

-

P

P

F

V

S

R

D

D

L

P

S

E

F

F

R

A

P

T

K

R

K

M

D

D

L

-

T

-

G

-

A

-

D

-

M

-

W

-

G

-

A

-

T

-

M

-

F

-

D

-

Q

-

L

-

V

-

C

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V

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F

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H

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Q

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I

-

F

-

T

-

P

-

P

-

S

-

E

H

Q

K

G

G

T

T

L

N

V

I

F

C

P

P

G

S

N

A

L

M

G

G

M

F

F

H

E
x
N

W

Q

G

G

G

V

I

D

S

S

V

Y

D

D

P

P

N

E

R

S

Q

R

V

T

A

L

I

Y

A

A

-

G

-

L

N

N

P

H

M

I

A

C

L

M

P

D

F

W

V

E

S

P

K

F

L

M

I

L

P

P

R

Y

G

R

P

A

G

G

N

-

P

-

M

-

E

-

Q

-

P

-

K

-

D

-

A

-

K

-

G

-

S

-

G

-

T

-

E

-

A

-

G

-

I

-

Q

-

P

-

Q

Q

Y

F

G

F

V

V

P

-

Y

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G

G

V

A

T

T

L

L

N

A

P

M

F

Y

L

P

S

G

P

P

F

N

G

G

L

-

P

P

C

T

K

K

Q

K

P

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A

M

W

-

G

G

Y

Q

I

I

S

R

A

A

L

F

D

D

L

L

K

T

T

T

N

G

E

K

V

A

V

K

W

W

K

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K

K

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W

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M

-

P

-

V

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K

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L

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F

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T

-

M

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G

K

M

F

P

A

M

A

L
x
W

G

G

G

G

P

T

I

L

S

Y

T

T

A

K

G

G

N

G

V

L

L

V

F

W

I

Y

G

-

A

A

T

T

A

L

D

D

N

G

Y

Y

L

L

R

K

A

A

Y

L

N

D

M