SitesBLAST
Comparing BWI76_RS06145 FitnessBrowser__Koxy:BWI76_RS06145 to proteins with known functional sites using BLASTp with E ≤ 0.001.
Or try Sites on a Tree, PaperBLAST, Conserved Domains, or compare to all protein structures
Found 14 hits to proteins with known functional sites (download)
P0AA76 D-galactonate transporter; D-galactonate/H(+) symporter from Escherichia coli (strain K12) (see paper)
25% identity, 95% coverage: 5:412/428 of query aligns to 7:427/430 of P0AA76
- Y29 (= Y27) binding
- D31 (= D29) mutation to N: Loss of galactonate transport activity.
- R32 (= R30) binding
- Y64 (= Y63) binding
- E118 (= E117) mutation to Q: Loss of galactonate transport activity.
- W358 (vs. gap) binding
6e9nA E. Coli d-galactonate:proton symporter in the inward open form (see paper)
24% identity, 94% coverage: 10:412/428 of query aligns to 1:408/409 of 6e9nA
6e9oA E. Coli d-galactonate:proton symporter mutant e133q in the outward substrate-bound form (see paper)
24% identity, 95% coverage: 8:412/428 of query aligns to 2:392/393 of 6e9oA
Q03567 Uncharacterized transporter slc-17.2 from Caenorhabditis elegans (see paper)
24% identity, 59% coverage: 49:301/428 of query aligns to 80:340/493 of Q03567
Sites not aligning to the query:
- 69 modified: carbohydrate, N-linked (GlcNAc...) asparagine
Q8BN82 Sialin; H(+)/nitrate cotransporter; H(+)/sialic acid cotransporter; AST; Solute carrier family 17 member 5; Vesicular excitatory amino acid transporter; VEAT from Mus musculus (Mouse) (see paper)
23% identity, 74% coverage: 59:376/428 of query aligns to 115:440/495 of Q8BN82
- H183 (≠ L125) mutation to R: Abolishes sialic acid transporter activity. Does not affect L-aspartate and L-glutamate transporter activity.
Sites not aligning to the query:
- 39 R→C: Completely abolishes L-aspartate and L-glutamate transporter activity. Retains appreciable H(+)-coupled sialic acid transporter activity.
P53322 High-affinity nicotinic acid transporter; Nicotinic acid permease from Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast) (see paper)
23% identity, 76% coverage: 2:328/428 of query aligns to 78:410/534 of P53322
- K283 (≠ E202) modified: Glycyl lysine isopeptide (Lys-Gly) (interchain with G-Cter in ubiquitin)
Q5Q0U0 Sialin; H(+)/nitrate cotransporter; H(+)/sialic acid cotransporter; AST; Solute carrier family 17 (Anion/sugar transporter), member 5; Vesicular excitatory amino acid transporter; VEAT from Rattus norvegicus (Rat) (see 2 papers)
22% identity, 74% coverage: 59:376/428 of query aligns to 115:440/495 of Q5Q0U0
- K136 (≠ R80) mutation to E: Markedly decreases H(+)-coupled sialic acid transporter activity.
- R168 (= R110) mutation to C: Abolishes H(+)-coupled sialic acid transporter activity.
- E171 (≠ L113) mutation to C: Decreases H(+)-coupled sialic acid transporter activity; when associated with C-175.
- G172 (= G114) mutation to C: Decreases protein levels and alters subcellular localization.
- E175 (= E117) mutation to C: Decreases H(+)-coupled sialic acid transporter activity; when associated with C-171.
- G176 (≠ A118) mutation to C: Decreases protein levels and alters subcellular localization.
- F179 (≠ M121) mutation to C: Decreases the affinity and transport rate for D-glucuronate. Does not affect H(+)-coupled sialic acid transporter activity.
- P180 (= P122) mutation to C: Decreases protein levels and alters subcellular localization.
- H183 (≠ L125) mutation to R: Abolishes H(+)-coupled sialic acid transporter activity.
- W186 (≠ I128) mutation to C: Abolishes H(+)-coupled sialic acid transporter activity.
- SSLKN 268:272 (≠ KVMDS 210:214) mutation Missing: Abolishes H(+)-coupled sialic acid transporter activity.
- P334 (= P277) mutation to R: Abolishes H(+)-coupled sialic acid transporter activity.
- G371 (vs. gap) mutation to V: Remains in the endoplasmic reticulum.
Sites not aligning to the query:
- 22:23 Dileucine internalization motif; LL→AA: Targeted to plasma membrane; sialic acid uptake strongly activated at acidic pH.
- 39 R→C: Markedly decreases H(+)-coupled sialic acid transporter activity.
Q9NRA2 Sialin; H(+)/nitrate cotransporter; H(+)/sialic acid cotransporter; AST; Membrane glycoprotein HP59; Solute carrier family 17 member 5; Vesicular excitatory amino acid transporter; VEAT from Homo sapiens (Human) (see 8 papers)
22% identity, 74% coverage: 59:376/428 of query aligns to 115:440/495 of Q9NRA2
- K136 (≠ R80) to E: in SD; alters intracellular localization, only partially targeted to lysosomes and mainly detected in LAMP1-negative vesicles and in the Golgi apparatus; completely devoid of L-aspartate and L-glutamate transporter activity, but retains appreciable H(+)-coupled sialic acid transporter activity; dbSNP:rs80338795
- H183 (≠ L125) to R: in ISSD; alters intracellular localization, only partially targeted to lysosomes and mainly detected in LAMP1-negative vesicles and in the Golgi apparatus; abolishes H(+)-coupled sialic acid transporter activity; has normal L-aspartate and L-glutamate transporter activity; dbSNP:rs119491109
- LL 198:199 (≠ AN 140:141) mutation to AA: Localizes in vesicular structures mainly concentrated in the perinuclear region.
- IL 266:267 (≠ LQ 208:209) mutation to LA: Localizes in vesicular structures mainly concentrated in the perinuclear region.
- SSLRN 268:272 (≠ KVMDS 210:214) natural variant: Missing (in ISSD; alters intracellular localization, only partially targeted to lysosomes and mainly detected in LAMP1-negative vesicles and in the Golgi apparatus; abolishes H(+)-coupled sialic acid transporter activity; has normal L-aspartate and L-glutamate transporter activity)
- G328 (= G271) to E: in ISSD; some patients may manifest a milder phenotype consistent with Salla disease; markedly decreases H(+)-coupled sialic acid transporter activity; abolishes L-aspartate and L-glutamate transporter activity; dbSNP:rs386833996
- P334 (= P277) to R: in ISSD; does not affect intracellular localization, targeted to lysosomes; abolishes H(+)-coupled sialic acid transporter activity; abolishes L-aspartate and L-glutamate transporter activity; dbSNP:rs119491110
- G371 (vs. gap) to V: in ISSD; abolishes H(+)-coupled sialic acid transporter activity; abolishes L-aspartate and L-glutamate transporter activity; dbSNP:rs777862172
Sites not aligning to the query:
- 22:23 Dileucine internalization motif; LL→AA: Targeted to plasma membrane.; LL→GG: Targeted to plasma membrane; sialic acid uptake strongly activated at acidic pH.
- 39 R → C: in SD; frequent variant in Finland; alters intracellular localization, only partially targeted to lysosomes and mainly detected in LAMP1-negative vesicles and in the Golgi apparatus; completely devoid of L-aspartate and L-glutamate transport activity, but retains appreciable H(+)-coupled sialic acid and nitrate transporter activity; dbSNP:rs80338794
Q9JI12 Vesicular glutamate transporter 2; VGluT2; Differentiation-associated BNPI; Differentiation-associated Na(+)-dependent inorganic phosphate cotransporter; Solute carrier family 17 member 6 from Rattus norvegicus (Rat) (see 2 papers)
22% identity, 58% coverage: 51:300/428 of query aligns to 123:378/582 of Q9JI12
- H128 (≠ G56) mutation to A: Greatly lowers L-glutamate transport.
- R184 (= R110) mutation R->A,E,K: Greatly lowers L-glutamate transport.
- E191 (= E117) mutation to A: Greatly lowers L-glutamate transport.; mutation E->D,Q: Lowers L-glutamate transport.
- R322 (≠ W244) mutation to A: Loss of L-glutamate release. Abolishes the chloride ion conductance.
Sites not aligning to the query:
- 88 R→A: Impairs synaptic transmission. Abolishes the chloride ion conductance.
Q51955 4-hydroxybenzoate transporter PcaK from Pseudomonas putida (Arthrobacter siderocapsulatus) (see 2 papers)
20% identity, 86% coverage: 9:378/428 of query aligns to 24:406/448 of Q51955
- D41 (≠ A26) mutation D->A,N: Abolishes 4-HBA transport.; mutation to E: Decrease in 4-HBA transport.
- D44 (= D29) mutation D->A,N: Abolishes 4-HBA transport.; mutation to E: Decrease in 4-HBA transport.
- G85 (≠ A71) mutation to V: Abolishes 4-HBA transport and chemotaxis.
- D89 (≠ V75) mutation to N: Abolishes 4-HBA transport and chemotaxis.
- G92 (≠ S78) mutation to A: Decrease in 4-HBA transport and chemotaxis.; mutation to C: No change in 4-HBA transport and chemotaxis.; mutation G->L,V: Abolishes 4-HBA transport and chemotaxis.; mutation to Q: Decrease in 4-HBA transport and strong decrease in chemotaxis.
- R124 (= R110) mutation to A: Abolishes 4-HBA transport.
- E144 (≠ N130) mutation to A: Strong decrease in 4-HBA transport.
- H183 (≠ R169) mutation to A: Decrease in 4-HBA transport and chemotaxis.
- D323 (= D293) mutation to N: Abolishes 4-HBA transport and chemotaxis.
- H328 (≠ R298) mutation to A: Decrease in 4-HBA transport and chemotaxis.; mutation to R: Decrease in 4-HBA transport and loss of chemotaxis.
- R386 (≠ S358) mutation to A: Strong decrease in 4-HBA transport.
- R398 (≠ A370) mutation to A: Abolishes 4-HBA transport.
Sites not aligning to the query:
- 444 H→A: No change in 4-HBA transport and chemotaxis.
7t3nA R184q/e191q mutant of rat vesicular glutamate transporter 2 (vglut2)
21% identity, 58% coverage: 51:300/428 of query aligns to 65:320/452 of 7t3nA
Sites not aligning to the query:
Q9C0U9 Uncharacterized transporter PB1C11.03 from Schizosaccharomyces pombe (strain 972 / ATCC 24843) (Fission yeast) (see paper)
25% identity, 53% coverage: 5:231/428 of query aligns to 92:327/570 of Q9C0U9
Sites not aligning to the query:
- 14 modified: Phosphoserine
Q9Y2C5 Probable small intestine urate exporter; Solute carrier family 17 member 4 from Homo sapiens (Human) (see 2 papers)
23% identity, 56% coverage: 62:300/428 of query aligns to 120:360/497 of Q9Y2C5
Sites not aligning to the query:
- 66 modified: carbohydrate, N-linked (GlcNAc...) asparagine; N→A: Loss of glycosylation and significant reduction in the transport of thyroid hormones T3 and T4; when associated with A-75 and A-90.
- 75 modified: carbohydrate, N-linked (GlcNAc...) asparagine; N→A: Loss of glycosylation and significant reduction in the transport of thyroid hormones T3 and T4; when associated with A-66 and A-90.
- 90 modified: carbohydrate, N-linked (GlcNAc...) asparagine; N→A: Loss of glycosylation and significant reduction in the transport of thyroid hormones T3 and T4; when associated with A-75 and A-90.
- 372 A → T: in dbSNP:rs11754288
Q5EXK5 3-hydroxybenzoate transporter MhbT from Klebsiella oxytoca (see paper)
22% identity, 68% coverage: 11:301/428 of query aligns to 19:322/452 of Q5EXK5
- D82 (≠ A74) mutation to A: Loss of activity.
- V311 (≠ W290) mutation to W: Loss of activity.
- D314 (= D293) mutation to A: Loss of activity.
Query Sequence
>BWI76_RS06145 FitnessBrowser__Koxy:BWI76_RS06145
MHMLAKLPARRWWYLMPIIFITYSLAYLDRANYGFAAAAGIESDLGITKGTASLIGALFF
LGYFFFQVPGAIYAVKRSVRKMIFCSLILWGFCAAATGFVSNIPMLMAIRFTLGVVEAAV
MPAMLIYISNWFTKTERSRANTFLILGNPVTVLWMSIVSGYLIQAWGWREMFIIEGIPAV
LWAVCWWILVRDKPSEVSWLNEQEKETLQKVMDSEQSHIKPVRNYGEALRSRNVIMLCAV
HALWSIGVYGFMMWMPSILKNAAQMDIVAVGWLAAVPYLAAIILMLTVSWLSDKFQNRKL
FIWPLLLIAAIAFFGSWMVGNQAFWLSYALLVIAAACMYAPYGPFFALIPELLPRNVSGV
SMGLINSFGALGAFLGAWLVGYLNGITGGPGVSYTFMAVALLSSVVLMFNVRANPQSSSP
AVAKRVVG
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SitesBLAST's Database
SitesBLAST's database includes
(1) SwissProt
entries with experimentally-supported functional features;
and (2) protein structures with bound ligands, from the
BioLip database.
by Morgan Price,
Arkin group
Lawrence Berkeley National Laboratory