SitesBLAST

SitesBLAST – Find functional sites

SitesBLAST

Comparing BWI76_RS06185 FitnessBrowser__Koxy:BWI76_RS06185 to proteins with known functional sites using BLASTp with E ≤ 0.001.

Or try Sites on a Tree, PaperBLAST, Conserved Domains, or compare to all protein structures

Found 20 (the maximum) hits to proteins with known functional sites (download)

P10880 Shikimate kinase 2; SK 2; Shikimate kinase II; SKII; EC 2.7.1.71 from Dickeya chrysanthemi (Pectobacterium chrysanthemi) (Erwinia chrysanthemi) (see paper)
52% identity, 96% coverage: 1:168/175 of query aligns to 1:168/173 of P10880

query
sites
P10880

M

M

T

T

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E

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P

I

I

F

F

L

M

V

V

G

G

P

A

R

R

G

G

C
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C

G

G

K
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K

T

T

V

V

G

G

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E

L

L

A

A

K

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A

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L

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Y

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E

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A

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D

D

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D

H

I

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F

L

M

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Q

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M

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A

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I

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E

E

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G

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A

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A

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A

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G

I

M

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L

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Y

Q

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N

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F

F

M

M

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N

H

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Y

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F

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M

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M

C13 (= C13) mutation to S: 66% of wild-type activity. Increase in substrates affinity.
K15 (= K15) mutation to M: Loss of activity. Increased thermostability.
D34 (= D34) mutation to N: Loss of activity.
C162 (≠ E162) mutation to S: No effect on activity and substrates affinity.

1e6cA K15m mutant of shikimate kinase from erwinia chrysanthemi (see paper)
51% identity, 96% coverage: 1:168/175 of query aligns to 1:168/170 of 1e6cA

query
sites
1e6cA

M

M

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F

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M

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V

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A

R
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R

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G

C

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x
M

T
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T

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V

V

G

G

H

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E

L

L

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Y

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D

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G

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M

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F

M

M

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A

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binding phosphate ion: R11 (= R11), G12 (= G12), M15 (≠ K15), T16 (= T16)

2shkB The three-dimensional structure of shikimate kinase from erwinia chrysanthemi complexed with adp (see paper)
48% identity, 96% coverage: 1:168/175 of query aligns to 1:158/162 of 2shkB

query
sites
2shkB

M

M

T

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I

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M

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V

G

G

P

A

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G

C
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C

G
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G

K
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K

T
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T

V

V

G

G

H

R

A

E

L

L

A

A

K

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A

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Y

K

E

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A

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D

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D

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M

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G

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M

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E

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binding adenosine-5'-diphosphate: G12 (= G12), C13 (= C13), G14 (= G14), K15 (= K15), T16 (= T16), T17 (= T17), R110 (= R110), Q145 (≠ A155), P147 (= P157)
binding magnesium ion: Q134 (≠ R144), A137 (= A147)

1shkA The three-dimensional structure of shikimate kinase from erwinia chrysanthemi (see paper)
45% identity, 96% coverage: 1:168/175 of query aligns to 1:153/158 of 1shkA

query
sites
1shkA

M

M

T

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P

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I

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F

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M

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V

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G

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C

C

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K

K

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L

A

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D

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M

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Q

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x
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M

binding magnesium ion: R92 (= R92), A93 (≠ E93), G95 (= G95), Q129 (≠ R144), A132 (= A147)

4y0aA Shikimate kinase from acinetobacter baumannii in complex with shikimate (see paper)
39% identity, 84% coverage: 5:151/175 of query aligns to 11:155/179 of 4y0aA

query
sites
4y0aA

I

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x
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x
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K

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E

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I

binding (3r,4s,5r)-3,4,5-trihydroxycyclohex-1-ene-1-carboxylic acid: M17 (≠ R11), D40 (= D34), F63 (= F57), R64 (= R58), G86 (= G79), G87 (= G80), L126 (≠ T122), R143 (= R139)
binding sulfate ion: G18 (= G12), A19 (≠ C13), G20 (= G14), K21 (= K15), T22 (= T16), E112 (≠ S105), R124 (= R120), R137 (≠ G133)

3mufA Shikimate kinase from helicobacter pylori in complex with shikimate-3- phosphate and adp (see paper)
27% identity, 95% coverage: 3:168/175 of query aligns to 1:160/160 of 3mufA

query
sites
3mufA

Q

Q

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M

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M

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binding adenosine-5'-diphosphate: G10 (= G12), S11 (≠ C13), G12 (= G14), K13 (= K15), S14 (≠ T16), S15 (≠ T17), M98 (≠ A102), R106 (= R110), G147 (≠ A155), G148 (≠ S156)
binding shikimate-3-phosphate: M9 (≠ R11), D32 (= D34), R56 (= R58), G78 (= G78), G79 (= G79), G80 (= G80), R115 (= R120), R131 (= R139)

3n2eA Crystal structure of helicobactor pylori shikimate kinase in complex with nsc162535 (see paper)
27% identity, 95% coverage: 3:169/175 of query aligns to 2:162/168 of 3n2eA

query
sites
3n2eA

Q

Q

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I

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L

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x
F

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M

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C

A

binding 7-amino-4-hydroxy-3-[(E)-(5-hydroxy-7-sulfonaphthalen-2-yl)diazenyl]naphthalene-2-sulfonic acid: F9 (≠ P10), R45 (≠ A46), F48 (≠ V49), R57 (= R58), G80 (= G79), G81 (= G80), M84 (≠ L83), R116 (= R120), R132 (= R139), L135 (= L142), Y136 (= Y143)

1zuiA Structural basis for shikimate-binding specificity of helicobacter pylori shikimate kinase (see paper)
27% identity, 95% coverage: 3:168/175 of query aligns to 1:158/158 of 1zuiA

query
sites
1zuiA

Q

Q

L

H

I

L

F

V

L

L

V

I

G

G

P

F

R
x
M

G

G

C

S

G

G

K

K

T

S

V

L

G

A

H

Q

A

E

L

L

A

G

K

L

V

A

R

L

S

K

F

L

K

E

F

V

A

L

D

D

T

T

D
|
D

H

M

E

I

L

I

Q

S

E

E

H

R

E

V

Q

G

R

L

T

S

V

V

A

R

A

E

I

I

V
x
F

Q

E

E

L

G

G

W

E

E

D

R

N

F

F

R
|
R

A

M

L

F

E

E

T

K

Q

N

A

L

L

I

E

D

R

E

V

L

A

K

Q

T

P

L

A

K

T

T

-

P

-

H

I

V

I

I

A

S

T

T

G
|
G

G

G

I

I

I

V

L

M

S

H

E

E

Y

N

N

-

R

-

Q

-

F

-

M

L

R

K

E

G

N

L

G

G

V

T

I

F

Y

Y

L

L

E

K

A

M

S

D

V

F

S

E

A

T

L

L

I

I

D

K

R

R

L

L

E

N

A

-

Y

-

P

-

K

Q

A

R

E
|
E

Q

K

R
|
R

P

P

T

L

L

L

T

N

G

N

K

L

P

T

I

-

R

-

E

-

E

Q

V

A

G

K

E

E

V

L

L

F

A

E

Q

K

R
|
R

E

Q

A

A

L

L

Y

Y

R

E

A

K

A

N

A

A

H

S

H

F

I

I

N

D

A

A

T

R

A

G

S

G

P

L

D

N

E

N

V

S

V

L

E

K

Q

Q

I

V

M

L

T

Q

T

F

V

I

binding (3r,4s,5r)-3,4,5-trihydroxycyclohex-1-ene-1-carboxylic acid: M9 (≠ R11), D32 (= D34), F47 (≠ V49), R56 (= R58), G78 (= G78), G79 (= G79), E111 (= E118), R113 (= R120), R129 (= R139)

P07547 Pentafunctional AROM polypeptide; EC 4.2.3.4; EC 2.5.1.19; EC 2.7.1.71; EC 4.2.1.10; EC 1.1.1.25 from Emericella nidulans (strain FGSC A4 / ATCC 38163 / CBS 112.46 / NRRL 194 / M139) (Aspergillus nidulans) (see 2 papers)
30% identity, 61% coverage: 5:111/175 of query aligns to 866:979/1583 of P07547

query
sites
P07547

I

I

F

Y

L

I

V

I

G

G

P

M

R

R

G

G

C

A

G

G

K

K

T

S

T

T

V

A

G

G

H

N

A

W

L

V

A

S

K

K

V

A

R

L

S

N

F

R

K

P

F

F

A

V

D

D

T

L

D

D

H

T

E

E

L

L

Q

E

E

T

H

V

E

E

Q

G

R

M

T

T

V

I

A

P

A

D

I

I

V

I

Q

K

Q

T

E

R

G

G

W

W

E

Q

R

G

F

F

R

R

A

N

L

A

E

E

T

L

Q

E

A

I

L

L

E

K

R

R

V

T

A

L

Q

K

P

E

A

R

T

S

-

R

-

G

-

Y

I

V

I

F

A

A

T

C

G

G

I

V

L

E

S

M

E

P

Y

E

N

A

R

R

Q

K

F

L

M

L

-

T

-

D

-

Y

-

H

R

K

E

T

N

K

G

G

V

N

V

V

I

L

Y

L

L

L

E

M

A

R

S

D

V

I

S

K

A

K

L

I

I

M

D

D

R

F

L

L

Sites not aligning to the query:

44:46 binding
81:84 binding
114:116 binding
119 binding
139:140 binding
161 binding
179:182 binding
190 binding
194 binding
271 binding
287 binding

1u8aA Crystal structure of mycobacterium tuberculosis shikimate kinase in complex with shikimate and adp at 2.15 angstrom resolution (see paper)
30% identity, 91% coverage: 7:166/175 of query aligns to 6:161/163 of 1u8aA

query
sites
1u8aA

L

L

V

V

G

G

P

L

R

P

G
|
G

C

S

G
|
G

K
|
K

T
x
S

T
|
T

V

I

G

G

H

R

A

R

L

L

A

A

K

K

V

A

R

L

S

G

F

V

K

G

F

L

A

L

D

D

T

T

D
|
D

H

V

E

A

L

I

Q

E

E

Q

H

R

E

T

Q

G

R

R

T

S

V

I

A

A

A

D

I

I

V
x
F

Q

A

Q

T

E

D

G

G

W

E

E

Q

R

E

F

F

R
|
R

A

R

L

I

E

E

T

E

Q

D

A

V

L

V

E

R

-

A

R

A

V

L

A

A

Q

D

P

H

A

D

T

G

I

V

I

L

A

S

T

L

G

G

G
|
G

I

A

I

V

L

T

S

S

E

P

Y

G

N

V

R

R

Q

A

F

A

M

L

R

A

E

G

N

H

G

-

V

T

V

V

I

V

Y

Y

L

L

E

E

A

I

S

S

V

A

S

A

A

E

L

G

I

V

D

R

R
|
R

L

T

E

G

A

G

Y

V

P

-

K

-

A

-

E

-

Q

-

R

R

P

P

T
x
L

L

L

T

A

G

G

K

P

P

D

I

R

R

A

E

E

E

K

V

Y

G

R

E

A

V

L

L

M

A

A

Q

K

R
|
R

E

A

A

P

L

L

Y

Y

R

R

A

R

A

V

A

A

H

T

H

M

I

R

I

V

N

D

A

T

T

N

-

R

A
x
R

S

N

P
|
P

D

G

E

A

V

V

E

R

Q

H

I

I

M

L

T

S

binding adenosine-5'-diphosphate: G11 (= G12), G13 (= G14), K14 (= K15), S15 (≠ T16), T16 (= T17), R109 (= R110), R150 (≠ A155), P152 (= P157)
binding (3r,4s,5r)-3,4,5-trihydroxycyclohex-1-ene-1-carboxylic acid: D33 (= D34), F48 (≠ V49), R57 (= R58), G79 (= G79), G80 (= G80), L116 (≠ T122), R133 (= R139)

3bafA Crystal structure of shikimate kinase from mycobacterium tuberculosis in complex with amp-pnp
30% identity, 91% coverage: 7:166/175 of query aligns to 6:163/165 of 3bafA

query
sites
3bafA

L

L

V

V

G

G

P

L

R
x
P

G

G

C

S

G
|
G

K
|
K

T
x
S

T
|
T

V

I

G

G

H

R

A

R

L

L

A

A

K

K

V

A

R

L

S

G

F

V

K

G

F

L

A

L

D

D

T

T

D
|
D

H

V

E

A

L

I

Q

E

E

Q

H

R

E

T

Q

G

R

R

T

S

V

I

A

A

A

D

I

I

V

F

Q

A

Q

T

E

D

G

G

W

E

E

Q

R

E

F

F

R

R

A

R

L

I

E

E

T

E

Q

D

A

V

L

V

E

R

-

A

R

A

V

L

A

A

Q

D

P

H

A

D

T

G

I

V

I

L

A

S

T

L

G

G

I

A

I

V

L

T

S

S

E

P

Y

G

N

V

R

R

Q

A

F

A

M

L

R

A

E

G

N

H

G

-

V

T

V

V

I

V

Y

Y

L

L

E

E

A

I

S

S

V

A

S

A

A

E

L

G

I

V

D

R

R
|
R

L

T

E

G

A

G

Y

N

P

-

K

-

A

-

E

T

Q

V

R
|
R

P

P

T

L

L

L

T

A

G

G

K

P

P

D

I

R

R

A

E

E

E

K

V

Y

G

R

E

A

V

L

L

M

A

A

Q

K

R
|
R

E

A

A

P

L

L

Y

Y

R

R

A

R

A

V

A

A

H

T

H

M

I

R

I

V

N

D

A

T

T

N

-

R

A

R

S

N

P

P

D

G

E

A

V

V

E

R

Q

H

I

I

M

L

T

S

binding phosphoaminophosphonic acid-adenylate ester: P10 (≠ R11), G13 (= G14), K14 (= K15), S15 (≠ T16), T16 (= T17), R109 (= R110), R116 (= R120)
binding (3r,4s,5r)-3,4,5-trihydroxycyclohex-1-ene-1-carboxylic acid: D33 (= D34), R135 (= R139)

2dfnA Structure of shikimate kinase from mycobacterium tuberculosis complexed with adp and shikimate at 1.9 angstrons of resolution (see paper)
30% identity, 91% coverage: 7:166/175 of query aligns to 6:163/165 of 2dfnA

query
sites
2dfnA

L

L

V

V

G

G

P

L

R
x
P

G
|
G

C
x
S

G
|
G

K
|
K

T
x
S

T
|
T

V

I

G

G

H

R

A

R

L

L

A

A

K

K

V

A

R

L

S

G

F

V

K

G

F

L

A

L

D

D

T

T

D
|
D

H

V

E

A

L

I

Q

E

E

Q

H

R

E

T

Q

G

R

R

T

S

V
x
I

A

A

A

D

I

I

V

F

Q

A

Q

T

E

D

G

G

W

E

E

Q

R

E

F
|
F

R
|
R

A

R

L

I

E

E

T

E

Q

D

A

V

L

V

E

R

-

A

R

A

V

L

A

A

Q

D

P

H

A

D

T

G

I

V

I

L

A

S

T

L

G
|
G

I

A

I

V

L

T

S

S

E

P

Y

G

N

V

R

R

Q

A

F

A

M

L

R

A

E

G

N

H

G

-

V

T

V

V

I

V

Y

Y

L

L

E

E

A

I

S

S

V

A

S

A

A

E

L

G

I

V

D

R

R
|
R

L

T

E

G

A

G

Y

N

P

-

K

-

A

-

E

T

Q

V

R
|
R

P

P

T

L

L

L

T

A

G

G

K

P

P

D

I

R

R

A

E

E

E

K

V

Y

G

R

E

A

V

L

L

M

A

A

Q

K

R
|
R

E

A

A

P

L

L

Y

Y

R

R

A

R

A

V

A

A

H

T

H

M

I

R

I

V

N

D

A

T

T

N

-

R

A
x
R

S

N

P
|
P

D

G

E

A

V

V

E

R

Q

H

I

I

M

L

T

S

binding adenosine-5'-diphosphate: G11 (= G12), S12 (≠ C13), G13 (= G14), K14 (= K15), S15 (≠ T16), T16 (= T17), R109 (= R110), R116 (= R120), R152 (≠ A155), P154 (= P157)
binding chloride ion: P10 (≠ R11), K14 (= K15), G79 (= G79)
binding (3r,4s,5r)-3,4,5-trihydroxycyclohex-1-ene-1-carboxylic acid: D33 (= D34), I44 (≠ V45), F56 (= F57), R57 (= R58), G78 (= G78), G79 (= G79), G80 (= G80), R135 (= R139)

1we2A Crystal structure of shikimate kinase from mycobacterium tuberculosis in complex with mgadp and shikimic acid (see paper)
30% identity, 91% coverage: 7:166/175 of query aligns to 6:163/165 of 1we2A

query
sites
1we2A

L

L

V

V

G

G

P

L

R
x
P

G
|
G

C

S

G
|
G

K
|
K

T
x
S

T
|
T

V

I

G

G

H

R

A

R

L

L

A

A

K

K

V

A

R

L

S

G

F

V

K

G

F

L

A

L

D

D

T

T

D
|
D

H

V

E

A

L

I

Q

E

E

Q

H

R

E

T

Q

G

R

R

T

S

V
x
I

A

A

A

D

I

I

V
x
F

Q

A

Q

T

E

D

G

G

W

E

E

Q

R

E

F

F

R

R

A

R

L

I

E

E

T

E

Q

D

A

V

L

V

E

R

-

A

R

A

V

L

A

A

Q

D

P

H

A

D

T

G

I

V

I

L

A

S

T

L

G
|
G

I

A

I

V

L

T

S

S

E

P

Y

G

N

V

R

R

Q

A

F

A

M

L

R

A

E

G

N

H

G

-

V

T

V

V

I

V

Y

Y

L

L

E

E

A

I

S

S

V

A

S

A

A

E

L

G

I

V

D

R

R
|
R

L

T

E

G

A

G

Y

N

P

-

K

-

A

-

E

T

Q

V

R
|
R

P

P

T
x
L

L

L

T

A

G

G

K

P

P

D

I

R

R

A

E

E

E

K

V

Y

G

R

E

A

V

L

L

M

A

A

Q

K

R
|
R

E

A

A

P

L

L

Y

Y

R

R

A

R

A

V

A

A

H

T

H

M

I

R

I

V

N

D

A

T

T

N

-

R

A

R

S

N

P

P

D

G

E

A

V

V

E

R

Q

H

I

I

M

L

T

S

binding adenosine-5'-diphosphate: P10 (≠ R11), G11 (= G12), G13 (= G14), K14 (= K15), S15 (≠ T16), T16 (= T17), R109 (= R110), R116 (= R120)
binding 3-dehydroshikimate: D33 (= D34), I44 (≠ V45), F48 (≠ V49), G78 (= G78), G79 (= G79), G80 (= G80), L118 (≠ T122), R135 (= R139)

4bqsA Crystal structure of mycobacterium tuberculosis shikimate kinase in complex with adp and a shikimic acid derivative. (see paper)
30% identity, 91% coverage: 7:166/175 of query aligns to 6:163/169 of 4bqsA

query
sites
4bqsA

L

L

V

V

G

G

P

L

R
x
P

G
|
G

C

S

G
|
G

K
|
K

T
x
S

T
|
T

V

I

G

G

H

R

A

R

L

L

A

A

K

K

V

A

R

L

S

G

F

V

K

G

F

L

A

L

D

D

T

T

D
|
D

H

V

E

A

L

I

Q

E

E

Q

H

R

E

T

Q

G

R

R

T

S

V

I

A

A

A

D

I

I

V

F

Q

A

Q

T

E

D

G

G

W

E

E

Q

R

E

F

F

R

R

A

R

L

I

E

E

T

E

Q

D

A

V

L

V

E

R

-

A

R

A

V

L

A

A

Q

D

P

H

A

D

T

G

I

V

I

L

A

S

T

L

G

G

I

A

I

V

L

T

S

S

E

P

Y

G

N

V

R

R

Q

A

F

A

M

L

R

A

E

G

N

H

G

-

V

T

V

V

I

V

Y

Y

L

L

E

E

A

I

S

S

V

A

S

A

A

E

L

G

I

V

D

R

R
|
R

L

T

E

G

A

G

Y

N

P

-

K

-

A

-

E

T

Q

V

R

R

P

P

T

L

L

L

T

A

G

G

K

P

P

D

I

R

R

A

E

E

E

K

V

Y

G

R

E

A

V

L

L

M

A

A

Q

K

R
|
R

E

A

A

P

L

L

Y

Y

R

R

A

R

A

V

A

A

H

T

H

M

I

R

I

V

N

D

A

T

T

N

-

R

A
x
R

S

N

P
|
P

D

G

E

A

V

V

E

R

Q

H

I

I

M

L

T

S

binding adenosine-5'-diphosphate: P10 (≠ R11), G11 (= G12), G13 (= G14), K14 (= K15), S15 (≠ T16), T16 (= T17), R109 (= R110), R152 (≠ A155), P154 (= P157)
binding (1R,6R,10S)-6,10-dihydroxy-2-oxabicyclo[4.3.1]deca-4(Z),7-diene-8-carboxylic acid: D33 (= D34), R135 (= R139)

2iyyA Shikimate kinase from mycobacterium tuberculosis in complex with shikimate-3-phosphate and so4 (see paper)
30% identity, 91% coverage: 7:166/175 of query aligns to 6:163/169 of 2iyyA

query
sites
2iyyA

L

L

V

V

G

G

P

L

R

P

G

G

C

S

G

G

K
|
K

T

S

T

T

V

I

G

G

H

R

A

R

L

L

A

A

K

K

V

A

R

L

S

G

F

V

K

G

F

L

A

L

D

D

T

T

D
|
D

H

V

E

A

L

I

Q
x
E

E

Q

H

R

E

T

Q

G

R

R

T
x
S

V
x
I

A

A

A

D

I

I

V

F

Q

A

Q

T

E

D

G

G

W

E

E

Q

R

E

F

F

R
|
R

A

R

L

I

E

E

T

E

Q

D

A

V

L

V

E

R

-

A

R

A

V

L

A

A

Q

D

P

H

A

D

T

G

I

V

I

L

A

S

T

L

G

G

G
|
G

I

A

I

V

L

T

S

S

E

P

Y

G

N

V

R

R

Q

A

F

A

M

L

R

A

E

G

N

H

G

-

V

T

V

V

I

V

Y

Y

L

L

E

E

A

I

S

S

V

A

S

A

A

E

L

G

I

V

D

R

R

R

L

T

E

G

A

G

Y

N

P

-

K

-

A

-

E

T

Q

V

R

R

P

P

T
x
L

L

L

T

A

G

G

K

P

P

D

I

R

R

A

E

E

E

K

V

Y

G

R

E

A

V

L

L

M

A

A

Q

K

R
|
R

E

A

A

P

L

L

Y

Y

R

R

A

R

A

V

A

A

H

T

H

M

I

R

I

V

N

D

A

T

T

N

-

R

A

R

S

N

P

P

D

G

E

A

V

V

E

R

Q

H

I

I

M

L

T

S

binding magnesium ion: E37 (≠ Q38), S43 (≠ T44), I44 (≠ V45)
binding phosphate ion: K14 (= K15), G79 (= G79)
binding shikimate-3-phosphate: K14 (= K15), D33 (= D34), R57 (= R58), G79 (= G79), G80 (= G80), L118 (≠ T122), R135 (= R139)

1zyuA Crystal structure of mycobacterium tuberculosis shikimate kinase in complex with shikimate and amppcp at 2.85 angstrom resolution (see paper)
30% identity, 91% coverage: 7:166/175 of query aligns to 6:163/168 of 1zyuA

query
sites
1zyuA

L

L

V

V

G

G

P

L

R
x
P

G
|
G

C

S

G
|
G

K
|
K

T
x
S

T
|
T

V

I

G

G

H

R

A

R

L

L

A

A

K

K

V

A

R

L

S

G

F

V

K

G

F

L

A

L

D

D

T

T

D
|
D

H

V

E

A

L

I

Q

E

E

Q

H

R

E

T

Q

G

R

R

T

S

V

I

A

A

A

D

I

I

V

F

Q

A

Q

T

E

D

G

G

W

E

E

Q

R

E

F

F

R
|
R

A

R

L

I

E

E

T

E

Q

D

A

V

L

V

E

R

-

A

R

A

V

L

A

A

Q

D

P

H

A

D

T

G

I

V

I

L

A

S

T

L

G

G

G
|
G

I

A

I

V

L

T

S

S

E

P

Y

G

N

V

R

R

Q

A

F

A

M

L

R

A

E

G

N

H

G

-

V

T

V

V

I

V

Y

Y

L

L

E

E

A

I

S

S

V

A

S

A

A

E

L

G

I

V

D

R

R
|
R

L

T

E

G

A

G

Y

N

P

-

K

-

A

-

E

T

Q

V

R

R

P
|
P

T
x
L

L

L

T

A

G

G

K

P

P

D

I

R

R

A

E

E

E

K

V

Y

G

R

E

A

V

L

L

M

A

A

Q

K

R
|
R

E

A

A

P

L

L

Y

Y

R

R

A

R

A

V

A

A

H

T

H

M

I

R

I

V

N

D

A
x
T

T

N

-

R

A
x
R

S

N

P
|
P

D

G

E

A

V

V

E

R

Q

H

I

I

M

L

T

S

binding phosphomethylphosphonic acid adenylate ester: P10 (≠ R11), G11 (= G12), G13 (= G14), K14 (= K15), S15 (≠ T16), T16 (= T17), R109 (= R110), T149 (≠ A153), R152 (≠ A155), P154 (= P157)
binding (3r,4s,5r)-3,4,5-trihydroxycyclohex-1-ene-1-carboxylic acid: D33 (= D34), R57 (= R58), G80 (= G80), P117 (= P121), L118 (≠ T122), R135 (= R139)

2iywA Shikimate kinase from mycobacterium tuberculosis in complex with mgatp, open lid (conf. B) (see paper)
29% identity, 92% coverage: 6:166/175 of query aligns to 5:163/178 of 2iywA

query
sites
2iywA

F

V

L

L

V

V

G

G

P

L

R

P

G
|
G

C

S

G
|
G

K
|
K

T
x
S

T
|
T

V

I

G

G

H

R

A

R

L

L

A

A

K

K

V

A

R

L

S

G

F

V

K

G

F

L

A

L

D

D

T

T

D

D

H

V

E

A

L

I

Q

E

E

Q

H

R

E

T

Q

G

R

R

T

S

V

I

A

A

A

D

I

I

V

F

Q

A

Q

T

E

D

G

G

W

E

E

Q

R

E

F

F

R

R

A

R

L

I

E

E

T

E

Q

D

A

V

L

V

E

R

-

A

R

A

V

L

A

A

Q

D

P

H

A

D

T

G

I

V

I

L

A

S

T

L

G

G

I

A

I

V

L

T

S

S

E

P

Y

G

N

V

R

R

Q

A

F

A

M

L

R

A

E

G

N

H

G

-

V

T

V

V

I

V

Y

Y

L

L

E

E

A

I

S

S

V

A

S

A

A

E

L

G

I

V

D

R

R
|
R

L

T

E

G

A

G

Y

N

P

-

K

-

A

-

E

T

Q

V

R

R

P

P

T

L

L

L

T

A

G

G

K

P

P

D

I

R

R

A

E

E

E

K

V

Y

G

R

E

A

V

L

L

M

A

A

Q

K

R

R

E

A

A

P

L

L

Y

Y

R

R

A

R

A

V

A

A

H

T

H

M

I

R

I

V

N

D

A

T

T

N

-

R

A
x
R

S

N

P
|
P

D

G

E

A

V

V

E

R

Q

H

I

I

M

L

T

S

binding adenosine-5'-triphosphate: G11 (= G12), G13 (= G14), K14 (= K15), S15 (≠ T16), T16 (= T17), R109 (= R110), R152 (≠ A155), P154 (= P157)

2iyvA Shikimate kinase from mycobacterium tuberculosis in complex with adp, open lid (conf. B) (see paper)
29% identity, 92% coverage: 6:166/175 of query aligns to 5:163/179 of 2iyvA

query
sites
2iyvA

F

V

L

L

V

V

G

G

P

L

R

P

G
|
G

C

S

G
|
G

K
|
K

T
x
S

T
|
T

V

I

G

G

H

R

A

R

L

L

A

A

K

K

V

A

R

L

S

G

F

V

K

G

F

L

A

L

D

D

T

T

D

D

H

V

E

A

L

I

Q

E

E

Q

H

R

E

T

Q

G

R

R

T

S

V

I

A

A

A

D

I

I

V

F

Q

A

Q

T

E

D

G

G

W

E

E

Q

R

E

F

F

R

R

A

R

L

I

E

E

T

E

Q

D

A

V

L

V

E

R

-

A

R

A

V

L

A

A

Q

D

P

H

A

D

T

G

I

V

I

L

A

S

T

L

G

G

I

A

I

V

L

T

S

S

E

P

Y

G

N

V

R

R

Q

A

F

A

M

L

R

A

E

G

N

H

G

-

V

T

V

V

I

V

Y

Y

L

L

E

E

A

I

S

S

V

A

S

A

A

E

L

G

I

V

D

R

R
|
R

L

T

E

G

A

G

Y

N

P

-

K

-

A

-

E

T

Q

V

R

R

P

P

T

L

L

L

T

A

G

G

K

P

P

D

I

R

R

A

E

E

E

K

V

Y

G

R

E

A

V

L

L

M

A

A

Q

K

R

R

E

A

A

P

L

L

Y

Y

R

R

A

R

A

V

A

A

H

T

H

M

I

R

I

V

N

D

A

T

T

N

-

R

A
x
R

S

N

P
|
P

D

G

E

A

V

V

E

R

Q

H

I

I

M

L

T

S

binding adenosine-5'-diphosphate: G11 (= G12), G13 (= G14), K14 (= K15), S15 (≠ T16), T16 (= T17), R109 (= R110), R152 (≠ A155), P154 (= P157)

6hqvA Pentafunctional arom complex from chaetomium thermophilum (see paper)
29% identity, 79% coverage: 5:143/175 of query aligns to 845:983/1555 of 6hqvA

query
sites
6hqvA

I

I

F

F

L

I

V

V

G

G

P

M

R

R

G

G

C

A

G

G

K

K

T

S

T

T

V

A

G

G

H

R

A

W

L

M

A

S

K

E

V

L

R

L

S

K

F

R

K

P

F

L

A

V

D

D

T

L

D
|
D

H

A

E

E

L

L

Q

E

E

R

H

R

E

E

Q

G

R

M

T

T

V

I

A

P

A

E

I

I

V

I

Q

R

Q

G

E

E

-

R

G

G

W

W

E

E

R

G

F

F

R

R

A

Q

L

A

E

E

T

L

Q

E

A

L

L

L

E

Q

R

D

V

V

A

I

Q

K

P

N

A

Q

T

S

-

K

-

G

-

Y

I

I

I

F

A

S

T

C

G

G

G
|
G

I

I

I

V

L

E

S

T

E

E

Y

A

N

A

R

R

Q

K

F

L

M

L

-

I

-

D

-

Y

-

H

R

K

E

N

N

G

G

G

V

P

V

V

I

L

Y

L

L

V

E

H

A

R

S

D

V

T

S

D

A

Q

L

V

I

V

D

E

R

Y

L

L

E

M

A

-

Y

-

P

-

K

R

A

D

E

K

Q

T

R

R

P

P

T

A

L

Y

T

S

G

-

K

-

P

-

I

-

R

-

E

E

E

N

V

I

G

R

E

E

V

V

L

Y

A

E

Q

R

R
|
R

E

K

A

P

L

W

Y

F

binding (3r,4s,5r)-3,4,5-trihydroxycyclohex-1-ene-1-carboxylic acid: D874 (= D34), G923 (= G79), G924 (= G80), R979 (= R139)

Sites not aligning to the query:

active site: 123, 145, 187, 243, 253, 257, 261, 264, 268, 280
binding (4S,5R)-4,5-dihydroxy-3-oxocyclohex-1-ene-1-carboxylic acid: 1060, 1062, 1181, 1224, 1232, 1242, 1243
binding glutamic acid: 139, 145, 187, 243, 257, 264, 280
binding nicotinamide-adenine-dinucleotide: 42, 44, 45, 76, 79, 107, 108, 109, 112, 132, 133, 135, 139, 140, 145, 154, 175, 176, 177, 180, 280
binding (3r,4s,5r)-3,4,5-trihydroxycyclohex-1-ene-1-carboxylic acid: 413, 562, 563, 1277, 1279, 1323, 1327, 1348, 1368, 1526
binding zinc ion: 187, 264, 280

7tbvA Crystal structure of the shikimate kinase + 3-dehydroquinate dehydratase + 3-dehydroshikimate dehydrogenase domains of aro1 from candida albicans (see paper)
28% identity, 66% coverage: 5:119/175 of query aligns to 6:127/682 of 7tbvA

query
sites
7tbvA

I

I

F

I

L

V

V

I

G

G

P

M

R

R

G

G

C

T

G

G

K

K

T

S

T

T

V

L

G

S

H

E

A

W

L

L

A

A

K

S

V

F

R

L

S

G

F

F

K

K

F

M

A

L

D

D

T

M

D

D

H

K

E

Y

L

L

Q

E

E

E

H

K

E

L

Q

G

R

T

T

G

V

I

A

K

A

S

I

L

V

I

Q

K

Q

A

E

K

G

G

W

W

E

E

R

Y

F

F

R

R

A

Q

L

E

E

E

T

A

-

I

-

V

-

A

-

K

Q

E

A

C

L

F

E

T

R

K

V

F

A

S

Q

K

P

-

A

G

T

Y

I

V

I

L

A

S

T

T

G

G

I

I

I

V

L

E

S

G

E

E

Y

D

N

A

R

R

Q

Q

F

Q

M

L

R

K

E

S

-

Y

-

A

-

D

-

N

N

G

G

G

V

I

V

V

I

L

Y

H

L

L

E

H

A

R

S

D

V

L

S

D

A

E

L

S

I

S

D

E

R

V

L

Q

E

E

A

V

Y

W

P

L

K

R

A

R

E

E

Q

K

Sites not aligning to the query:

binding magnesium ion: 300, 302, 329

Query Sequence

>BWI76_RS06185 FitnessBrowser__Koxy:BWI76_RS06185
MTQLIFLVGPRGCGKTTVGHALAKVRSFKFADTDHELQEHEQRTVAAIVQQEGWERFRAL
ETQALERVAQPATIIATGGGIILSEYNRQFMRENGVVIYLEASVSALIDRLEAYPKAEQR
PTLTGKPIREEVGEVLAQREALYRAAAHHIINATASPDEVVEQIMTTVCGVSYTS

Or try a new SitesBLAST search

SitesBLAST's Database

SitesBLAST's database includes (1) SwissProt entries with experimentally-supported functional features; and (2) protein structures with bound ligands, from the BioLip database.

by Morgan Price, Arkin group
Lawrence Berkeley National Laboratory