SitesBLAST

R26 (= R14) mutation R->G,S,Q: Strong decrease in phenylalanine transport activity.
P54 (= P42) mutation to A: 50% of wild-type phenylalanine transport activity.; mutation to G: No change in phenylalanine transport activity.; mutation to L: 26% of wild-type phenylalanine transport activity.
F87 (= F75) mutation to L: No effect on phenylalanine transport activity.
F90 (≠ Y78) mutation to L: 65% of wild-type phenylalanine transport activity.
Y92 (≠ Q80) mutation to L: 41% of wild-type phenylalanine transport activity.
Y94 (= Y82) mutation to L: 69% of wild-type phenylalanine transport activity.
W95 (≠ L83) mutation to L: 10% of wild-type phenylalanine transport activity.
F98 (≠ L86) mutation to L: No effect on phenylalanine transport activity.
F101 (≠ Y89) mutation to L: 38% of wild-type phenylalanine transport activity.
W105 (= W93) mutation to L: 39% of wild-type phenylalanine transport activity.
Y107 (= Y95) mutation to L: No effect on phenylalanine transport activity.
W108 (≠ C96) mutation to L: 71% of wild-type phenylalanine transport activity.
F111 (≠ I99) mutation to L: 60% of wild-type phenylalanine transport activity.; mutation to Y: Enables the transport of tryptophan to almost the same steady-state level as that of phenylalanine.
E118 (≠ D106) mutation E->G,L,V,N: Loss of activity.
K168 (= K156) mutation K->L,R: Strong decrease in phenylalanine transport activity.; mutation to N: Loss of activity.
E226 (= E216) mutation E->A,Q,K,R,W: Loss of activity.
R252 (= R242) mutation R->D,E,F,W,P: Loss of activity.
P341 (= P331) mutation to A: 5% of wild-type phenylalanine transport activity.; mutation P->G,Q,K,R: Loss of activity.; mutation to S: 3% of wild-type phenylalanine transport activity.; mutation to T: 17% of wild-type phenylalanine transport activity.
P442 (≠ F433) mutation to A: 46% of wild-type phenylalanine transport activity.; mutation to G: 52% of wild-type phenylalanine transport activity.; mutation to L: 43% of wild-type phenylalanine transport activity.

P15993 Aromatic amino acid transport protein AroP; Aromatic amino acid:H(+) symporter AroP; General aromatic amino acid permease; General aromatic transport system from Escherichia coli (strain K12) (see paper)
45% identity, 99% coverage: 2:451/456 of query aligns to 6:452/457 of P15993

query
sites
P15993

E

Q

S

H

S

G

N

E

K

Q

L

L

K

K

R

R

G

G

L

L

S

K

T

N

R

R

H

H

I

I

R

Q

F

L

M

I

A

A

L

L

G

G

S

G

A

A

I

I

G

G

T

T

G

G

L

L

F

F

Y

L

G

G

S

S

A

A

D

S

A

V

I

I

K

Q

M

S

A

A

G

G

P

P

S

G

V

I

L

I

L

L

A

G

Y

Y

I

A

I

I

G

A

G

G

V

F

A

I

A

A

Y

F

I

L

I

I

M

M

R

R

A

Q

L

L

G

G

E

E

M

M

S

V

V

V

H

E

N

E

P

P

A

V

A

A

S

G

S

S

F

F

S

S

R

H

Y

F

A

A

Q

Y

D

K

Y

Y

L

W

G

G

P

S

L

F

A

A

G

G

Y

F

I

A

T

S

G

G

W

W

T

N

Y

Y

C

W

F

V

E

L

I
x
Y

L

V

I

L

V

V

A

A

I

M

A

A

D

E

V

L

T

T

A

A

F

V

G

G

I

K

Y

Y

M

I

G

Q

V

F

W

W

F

Y

P

P

A

E

V

I

P

P

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T

W

W

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V

W

S

V

A

L

A

S

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F

L

V

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V

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C

N

A

A

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I

N

N

L

L

M

T

S

N

V

V

K

K

V

V

F

F

G

G

E

E

L

M

E

E

F

F

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W

F

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A

F

I

K

K

V

V

A

I

T

A

I

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I

A

M

M

I

I

L

I

A

-

G

-

F

F

G

G

I

G

I

W

I

L

W

L

G

F

I

S

G

G

N

N

G

G

Q

P

P

Q

T

A

G

T

I

V

H

S

N

N

L

L

W

W

S

D

N

Q

G

G

F

F

F

L

S

P

N

H

G

G

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F

L

T

G

G

M

L

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V

M

M

S

M

L

M

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A

M

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I

M

M

F

F

A

S

Y

F

G

G

I

L

E

E

I

L

I

V

G

G

I

I

T

T

A

A

G

A

E

E

A

A

K

D

D

N

P

P

E

E

K

Q

S

S

I

I

P

P

R

K

A

A

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T

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N

S

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V

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M

Y

R

R

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L

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F

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Y

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L

F

A

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P

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F

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F

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H

H

E

L

L

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G

I

D

T

T

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F

A

V

A

A

S

N

I

A

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L

N

N

F

I

V

V

L

L

T

T

A

A

S

A

L

L

S

S

A

V

I

Y

N

N

S

S

D

C

V

V

F

Y

G

C

V

N

G

S

R

R

M

M

L

L

H

F

G

G

M

L

A

A

E

Q

Q

Q

G

G

S

N

A

A

P

P

K

K

M

A

F

L

A

A

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D

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R

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G

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P

W

V

V

N

T

T

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L

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V

M

S

T

A

I

L

A

V

L

T

L

A

F

L

A

C

V

V

Y

L

L

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N

Y

Y

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A

P

P

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E

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S

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A

F

F

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G

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L

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S

A

L

L

A

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T

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F

S

A

A

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L

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N

W

W

I

A

M

M

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I

L

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L

L

S

A

Q

H

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M

A

K

F

F

R

R

R

A

L

-

P

K

P

Q

E

E

E

Q

A

G

K

V

A

V

L

T

K

R

F

F

K

P

V

A

P

L

G

L

G

Y

V

P

T

L

T

G

T

N

V

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C

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L

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F

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A

F

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M

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D

G

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A

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W

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L

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L

L

L

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L

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F

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K

T

E

R

K

R

T

E

A

R

K

Y103 (≠ I99) Key residue for tryptophan transport; mutation to F: Decreases tryptophan transport to less than 50% of wild-type levels and reduces the ability of tryptophan to inhibit phenylalanine transport from 95 to 62%.

P46349 Gamma-aminobutyric acid permease; GABA permease; 4-aminobutyrate permease; Gamma-aminobutyrate permease; Proline transporter GabP from Bacillus subtilis (strain 168) (see paper)
40% identity, 87% coverage: 2:397/456 of query aligns to 3:397/469 of P46349

query
sites
P46349

E

Q

S

S

S

Q

N

S

K

G

L

L

K

K

R

K

G

E

L

L

S

K

T

T

R

R

H

H

I

M

R

T

F

M

M

I

A

S

L

I

G

A

S

G

A

V

I

I

G

G

T

A

G

G

L

L

F

F

Y

V

G
|
G

S

S

A

G

D

S

A

V

I

I

K

H

M

S

A

T

G
|
G

P

P

S

G

V

A

L

V

A

S

Y

Y

I

A

I

L

G

A

G

G

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L

A

L

A

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F

I

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M

R

R

A

M

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L

G

G

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E

M

M

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S

V

A

H

V

N

N

P

P

A

T

A

S

S

G

S

S

F

F

S

S

R

Q

Y

Y

A

A

Q

H

D

D

Y

A

L

I

G

G

P

P

L

W

A

A

G

G

Y

F

I

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T

I

G

G

W

W

T

L

Y

Y

-

W

C

F

F

F

E

W

I

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L

I

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V

I

A

A

I

I

A

E

D

A

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A

A

G

F

A

G

G

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M

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Y

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W

F

F

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H

A

D

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P

H

L

W

W

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L

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S

L

L

S

I

V

L

V

T

L

I

I

V

I

L

C

T

A

L

V

T

N

N

L

V

M

Y

S

S

V

V

K

K

V

S

F

F

G

G

E

E

L

F

E

E

F

Y

W

W

F

F

S

S

F

L

F

I

K

K

V

V

A

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T

I

I

I

A

M

F

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L

I

A

V

G

G

F

F

G

A

I

F

I

I

I

-

W

F

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G

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F

G

A

N

P

G

G

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E

P

P

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V

G

G

I

F

H

S

N

N

L

L

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G

N

K

G

G

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N

E

G

G

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M

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V

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V

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G

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V

G

A

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A

A

A

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G

E

E

A

T

K

S

D

N

P

P

E

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K

A

A

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R

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S

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R

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F

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Y

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L

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F

A

V

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I

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V

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A

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L

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P

W

W

N

N

Q

S

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A

G

N

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N

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E

S

S

P

P

F

F

V

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A

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F

L

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E

H

H

L

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G

G

I

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T

P

F

A

A

A

S

Q

I

I

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M

N

N

F

F

V

I

V

V

L

L

T

T

A

A

S

V

L

L

S

S

A

C

I

L

N

N

S

S

D
x
G

V

L

F

Y

G

T

V

T

G

S

R

R

M

M

L

L

H

Y

G

S

M

L

A

A

E

E

Q

R

G

N

S

E

A

A

P

P

K

R

M

R

F

F

A

M

K

K

T

L

S

S

R

K

G

G

V

V

P

P

W

V

V

Q

T

A

V

I

M

V

V

A

M
x
G

T

T

I

F

A
x
F

L

S

L

Y

F

I

A

A

V

V

Y

V

L

M

N

N

Y

Y

I

F

M

S

P

P

E

D

N

T

V

V

F

F

L

L

V

F

I

L

A

V

S

N

L

S

A

S

T

G

F

A

A

I

T

A

V

L

W

L

V

V

W

Y

I

L

M

V

I

I

L

A

L

V

S

S

Q

Q

I

L

A

K

F

M

R

R

R

K

L

L

P

E

P

K

E

T

E

N

A

P

K

E

A

A

L

L

K

K

F

I

K

K

G33 (= G32) mutation to D: Lack of activity.
G42 (= G41) mutation to S: Lack of activity.
G301 (≠ D301) mutation to V: Lack of activity.
G338 (≠ M338) mutation to E: Lack of activity.
F341 (≠ A341) mutation to S: Lack of activity.

Sites not aligning to the query:

414 G→R: Lack of activity.

P25737 Lysine-specific permease LysP; Lysine transporter LysP; Trigger transporter LysP from Escherichia coli (strain K12) (see 2 papers)
38% identity, 83% coverage: 7:384/456 of query aligns to 13:398/489 of P25737

query
sites
P25737

L

L

K

R

R

R

G

E

L

L

S

K

T

A

R

R

H

H

I

L

R

T

F

M

M

I

A

A

L

I

G

G

S

G

A

S

I

I

G

G

T

T

G

G

L

L

F

F

Y

V

G

A

S

S

A

G

D

A

A

T

I

I

K

S

M

Q

A

A

G

G

P

P

S

G

-

G

V

A

L

L

A

S

Y

Y

I

M

I

L

G

I

G

G

V

L

A

M

A

V

Y

Y

I

F

I

L

M

M

R

T

A

S

L

L

G

G

E

E

M

L

S

A

V

A

H

Y

N

M

P

P

A

V

A

S

S

G

S

S

F

F

S

A

R

T

Y

Y

A

G

Q

Q

D

N

Y

Y

L

V

G

E

P

E

L

G

A

F

G

G

Y

F

I

A

T

L

G

G

W

W

T

N

Y
|
Y

C

W

F

Y

E

N

I
x
W

L

A

I

V

V

T

A

I

I

A

A

V

D

D

V

L

T

V

A

A

F

A

G

Q

I

L

Y

V

M

M

G

S

V

W

W

W

F

F

P

P

A

D

V

T

P

P

H

G

W

W

V

I

W

W

V

S

L

A

S

L

V

F

V

L

L

G

I

V

I

I

C

F

A

L

V

L

N

N

L

Y

M

I

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S

V

V

K

R

V

G

F

F

G

G

E

E

L

A

E

E

F

Y

W

W

F

F

S

S

F

L

F

I

K
|
K

V

V

A

T

T

T

I

V

I

I

I

V

M

F

I

I

L

I

A

V

G

G

F

V

G

L

I

M

I

I

W

-

G

G

I

I

G

F

N

K

G

G

G

A

Q

Q

P

P

T

A

G

G

I

W

H

S

N

N

L

W

W

T

S

I

N

G

G

E

G

A

F

P

F

F

S

A

N

G

G

G

W

F

L

A

G

A

M

M

V

I

M

G

S

V

L

A

Q

M

M

I

V

V

M

G

F
|
F

A

S

Y

F

G

Q

G

G

I

T

E
|
E

I

L

I

I

G

G

I

I

T

A

A

A

G

G

E
|
E

A

S

K

E

D

D

P

P

E

A

K

K

S

N

I

I

P

P

R

R

A

A

I

V

N

R

S

Q

V

V

P

F

M

W

R

R

I

I

L

L

V

L

F

F

Y

Y

V

V

G

F

T

A

L

I

F

L

V

I

I

I

M

S

S

L

I

I

Y

I

P

P

W

Y

-

T

-

D

-

P

-

S

-

L

-

R

N

N

Q
x
D

V

V

G

K

T
x
D

-

I

N

S

G

V

S

S

P

P

F

F

V

T

L

L

T

V

F

F

Q

Q

H

H

L

A

G

G

I

L

T

L

F

S

A

A

S

A

I

V

L

M

N

N

F

A

V

V

V

I

L

L

T

T

A

A

S

V

L

L

S

S

A

A

I

G

N

N

S

S

D

G

V

M

F

Y

G

A

V

S

G

T

R

R

M

M

L

L

H

Y

G

T

M

L

A

A

E

C

Q

D

G

G

S

K

A

A

P

P

K

R

M

I

F

F

A

A

K

K

T

L

S

S

R

R

R

G

G

G

V

V

P

P

-

R

-

N

-

A

-

L

W

Y

V

A

T

T

V

T

M

V

V

I

M

A

T

G

I

L

A

C

L

F

L

L

F

T

A

S

V

M

Y

F

L

G

N

N

Y

-

I

-

M

-

P

-

E

Q

N

T

V

V

F

Y

L

L

V

W

I

L

A

L

S

N

L

T

A

S

T

G

F

M

A

T

T

G

V

F

W

I

V

A

W

W

I

L

M

G

I

I

L

A

L

I

S

S

Q

H

I

Y

A

R

F

F

R

R

Y102 (= Y95) mutation to L: Retains 4% of wild-type lysine uptake activity. Increases the capacity to inhibit CadC in the presence of lysine.
W106 (≠ I99) mutation to L: Retains 20% of wild-type lysine uptake activity. Increases the capacity to inhibit CadC in the presence of lysine.
K163 (= K156) mutation to A: Retains 24% of wild-type lysine uptake activity. Increases the capacity to inhibit CadC in the presence of lysine.
F216 (= F210) mutation to L: Retains 13% of wild-type lysine uptake activity. Increases the capacity to inhibit CadC in the presence of lysine.
E222 (= E216) mutation to A: Abolishes lysine uptake. Strongly inhibits CadC.
E230 (= E224) mutation to V: Abolishes lysine uptake. Shows significant less inhibition of CadC.
D275 (≠ Q262) Essential for the stimulus-dependent interaction with CadC; mutation to A: Retains 88% of wild-type lysine uptake activity, but can hardly inhibit CadC. Cannot interact with CadC; when associated with A-278.
D278 (≠ T265) Essential for the stimulus-dependent interaction with CadC; mutation to A: Retains 88% of wild-type lysine uptake activity, but can hardly inhibit CadC. Cannot interact with CadC; when associated with A-275.

Sites not aligning to the query:

1 modified: Initiator methionine, Removed
438 E→A: Retains 14% of wild-type lysine uptake activity. Is unable to inhibit CadC.
443 D→A: Retains 11% of wild-type lysine uptake activity. Is unable to inhibit CadC.
446 D→A: Retains 13% of wild-type lysine uptake activity. Is unable to inhibit CadC.

P04817 Arginine permease CAN1; Canavanine resistance protein 1 from Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast) (see paper)
33% identity, 96% coverage: 6:443/456 of query aligns to 83:541/590 of P04817

query
sites
P04817

K

E

L

V

K

K

R

R

G

E

L

L

S

K

T

Q

R

R

H

H

I

I

R

G

F

M

M

I

A

A

L

L

G

G

S

G

A

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I

I

G

G

T

T

G

G

L

L

F

F

Y

I

G

G

S

L

A

S

D

T

A
x
P

I

L

K

T

M

N

A

A

G

G

P

P

-

V

S

G

V

A

L

L

L

I

A

S

Y

Y

I

L

I

F

G

M

G

G

V

S

A

L

A

A

Y

Y

I

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I

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R

Q

A

S

L

L

G

G

E

E

M

M

S

A

V

T

H

F

N

I

P
|
P

A
x
V

A

T

S

S

S
|
S

F

F

S

T

R

V

Y

F

A

S

Q

Q

D

R

Y

F

L

L

G

S

P

P

L

A

A

F

G

G

Y

A

I

A

T

N

G

G

W

Y

T

M

Y
|
Y

C

W

F

F

E

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I

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L

A

I

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T

A

F

I

A

A

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D

E

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L

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A

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F

V

G

G

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Y

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M

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Q

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F

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W

F

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Y

A

K

V

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P

P

H

L

W

A

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A

W

W

V

I

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S

S

I

V

F

V

W

L

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I

I

C

T

A

I

V

M

N

N

L

L

M

F

S

P

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K

K

V

Y

F

Y

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G

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E

L

F

E

E

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F

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A

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S

F

I

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K

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V

A

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A

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I

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I

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L

I

A

Y

G

C

F

F

G

C

I

M

I

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C

W

-

G

G

I

A

G

G

N

V

G

T

G

G

Q

-

P

P

T

V

G

G

I

F

H

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N

-

L

Y

W

W

S

R

N

N

G

P

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G

F

A

F

W

S

G

N

P

G

G

-

I

-

S

-

K

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D

-

K

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-

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-

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-

R

W

F

L

L

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W

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V

M

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S

S

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L

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M

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V

A

M

A

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F

A

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Y

F

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Q

G

G

I

T

E

E

I

L

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G

G

I

I

T

T

A

A

G
|
G

E

E

A

A

K

A

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N

P
|
P

E

R

K

K

S

S

I

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P

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R

A

A

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I

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K

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R

I

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F

Y

Y

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G

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S

L

L

F

L

V

F

I

I

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G

S

L

I

L

Y

V

P

P

W

Y

N

N

Q

D

V

P

G

K

-

L

-

T

-

Q

-

S

-
x
T

-
x
S

-
x
Y

T

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N

S

G

T

S

S

P

P

F

F

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A

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V

V

I

L

L

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T

A

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S

I

L

I

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S

A

A

I

A

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R

G

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P

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Y

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A

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M

F

V

V

M

T

T

A

I

A

A

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L

G

L

A

F

L

A

A

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Y

Y

M

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E

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T

Y

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P

G

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D

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x
W

I

L

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N

L

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A

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T

G

F

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A

A

T

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x
F

W

F

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A

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W

I

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F

I

I

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L

I

S

S

Q

H

I

I

A

R

F

F

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M

R

Q

R

A

L

L

P

K

P

Y

E

R

-

G

-

I

E

S

A

R

K

D

A

E

L

L

K

P

F

F

K

K

-

A

-

K

-

L

V

M

P

P

G

G

G

L

V

A

T

Y

T

A

V

A

I

T

G

F

L

M

L

T

F

I

L

I

V

I

F

I

I

I

I

Q

G

G

L

F

I

T

G

A

Y

F

H

A

P

P

D

K

-

F

T

N

R

G

I

V

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S

L

F

Y

A

V

A

G

A

F

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I

I

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L

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L

L

I

A

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V

W

W

P113 (≠ A36) mutation to L: In CAN1-343; confers citrulline transport activity in GAP1-deleted cells.
P148 (= P70) mutation to L: In CAN1-337; confers citrulline transport activity in GAP1-deleted cells and leads to sensitivity to L-glutamic acid alpha-hydroxamate, alpha-aminoisobutyrate, 3-chloro-L-alanine, L-ethionine, L-allylglycine, and D-histidine, but not sensitivity to L-aspartic acid alpha-hydroxamate or p-fluoro-L-phenylalanine.
V149 (≠ A71) mutation to F: In CAN1-315; confers citrulline transport activity in GAP1-deleted cells.
S152 (= S74) mutation to F: In CAN1-342; confers citrulline transport activity in GAP1-deleted cells.
Y173 (= Y95) mutation to D: In CAN1-306; confers citrulline transport activity in GAP1-deleted cells.; mutation to H: In CAN1-327; confers citrulline transport activity in GAP1-deleted cells.
G308 (= G223) mutation to A: In CAN1-341; confers citrulline transport activity in GAP1-deleted cells.
P313 (= P228) mutation to S: In CAN1-329; confers citrulline transport activity in GAP1-deleted cells and leads to sensitivity to L-glutamic acid alpha-hydroxamate, alpha-aminoisobutyrate, 3-chloro-L-alanine, L-ethionine, L-allylglycine, and D-histidine, L-aspartic acid alpha-hydroxamate and p-fluoro-L-phenylalanine.
TS 354:355 (vs. gap) mutation Missing: In CAN1-318; confers citrulline transport activity in GAP1-deleted cells.
Y356 (vs. gap) mutation to H: In CAN1-340; confers citrulline transport activity in GAP1-deleted cells.; mutation to N: In CAN1-339; confers citrulline transport activity in GAP1-deleted cells.
W451 (≠ V359) mutation to C: In CAN1-328; confers citrulline transport activity in GAP1-deleted cells.; mutation to L: In CAN1-316; confers citrulline transport activity in GAP1-deleted cells.; mutation to S: In CAN1-335; confers citrulline transport activity in GAP1-deleted cells.
F461 (≠ V369) mutation to S: In CAN1-307; confers citrulline transport activity in GAP1-deleted cells.

Q9URZ4 Cationic amino acid transporter 1 from Schizosaccharomyces pombe (strain 972 / ATCC 24843) (Fission yeast) (see paper)
32% identity, 91% coverage: 2:416/456 of query aligns to 75:501/587 of Q9URZ4

query
sites
Q9URZ4

E

E

S

D

S

G

N

T

K

A

L

L

K

K

R

R

G

S

L

L

S

K

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S

R

R

H

H

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R

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M

M

I

A

S

L

I

G

G

S

G

A

A

I

I

G

G

T

T

G

G

L

L

F

Y

Y

V

G

G

S

S

A

G

D

S

A

S

I

L

K

A

M

D

A

G

G

G

P

P

-

A

S

S

V

V

L

I

A

N

Y

Y

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S

I

L

G

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G

G

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I

A

M

Y

F

I

F

I

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R

Y

A

A

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L

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G

E

E

M

M

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A

V

V

H

A

N

Y

P

P

A

V

A

A

S

G

F

F

S

N

R

T

Y

Y

A

A

Q

T

D

R

Y

F

L

I

G

D

P

P

L

A

A

W

G

G

Y

F

I

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G

S

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W

T

N

Y

Y

C

F

F

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E

N

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L

F

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A

F

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P

A

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L

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C

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A

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F

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L

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C

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A

I

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N

N

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A

F

Y

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G

E

E

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E

E

F

F

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F

L

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S

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F

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K

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A

-

T

-

I

-

M

-

I

-

L

V

A

A

G

T

F

F

G

G

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F

I

I

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L

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A

I

I

-

I

G

N

N

C

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D

I

-

H

H

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Y

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I

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K

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-

K

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P

F

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N

H

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F

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M

A

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Y

F

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I

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A

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L

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L

I

I

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-

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S

I

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Y

D

P

D

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P

N

N

Q

L

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M

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-

N

-

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-

S

T

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N

S

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S

S

P

P

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F

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V

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A

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L

A

G

N

I

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G

A

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S

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F

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N

F

A

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S

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L

V

S

S

A

V

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N

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S

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F

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A

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H

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A

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F

F

A

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L

I

L

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F

L

G

L

F

F

F

A

A

V

-

Y

Y

L

I

N

N

Y

E

I

A

-

D

-

K

-

N

-

G

-

N

-

D

M

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F

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I

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A

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I

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F

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A

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P

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E

Q

-

G

-

H

E

S

A

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A

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F

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L

L

F

F

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F

L

I

C

I

L

Sites not aligning to the query:

29 modified: Phosphoserine
30 modified: Phosphoserine
37 modified: Phosphoserine

P19145 General amino-acid permease GAP1 from Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast) (see 3 papers)
29% identity, 98% coverage: 3:449/456 of query aligns to 82:553/602 of P19145

query
sites
P19145

S

A

S

Q

N

T

K

P

L

L

K

K

R

H

G

H

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L

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N

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H

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A

A

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I

G

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I

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A

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M

A

A

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E

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A

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F

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P

A

I

A

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Y

Y

A

A

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D

R

Y

F

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P

E

L

S

A

F

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Y

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G

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A

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A

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A

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M

V

G

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V

F

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W

F

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T

A

D

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P

P

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H

Y

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D

W

G

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F

L

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S

A

V

L

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F

-

W

L

L

I

A

I

I

C

V

A

I

V

I

N

N

L

M

M

F

S

G

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V

K

K

V

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F

Y

G

G

E

E

L

A

E

E

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F

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V

F

F

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S

F

F

F

I

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K

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V

A

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M

F

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L

I

A

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G

-

F

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G

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N

G

C

I

G

G

G

N

G

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Q

G

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Y

G

I

I

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H

G

N

K

L

Y

W

W

S

H

N

D

G

P

G

G

F

A

F

F

S

A

N

G

G

D

-

T

-

P

-

G

-

A

-

K

W

F

L

K

G

G

M

V

V

C

M

S

S

V

L

F

Q

V

M

T

V

A

M

A

F

F

A

S

Y

F

G
x
A

G

G

I

S

E

E

I

L

I

V

G

G

I

L

T

A

A

A

G

S

E

E

A

S

K

V

D

E

P

P

E

R

K

K

S

S

I

V

P

P

R

K

A

A

I

A

N

K

S

Q

V

V

P

F

M

W

R

R

I

I

L

T

V

L

F

F

Y

Y

V

I

G

L

T

S

L

L

F

L

V

M

I

I

M

G

S

L

I

L

Y

V

P

P

W

Y

N

N

Q

D

-

K

-

S

-

L

-

I

-

G

-

A

-

S

V

V

G

D

T

A

N

A

G

A

S

S

P

P

F

F

V

V

L

I

T

A

F

I

Q

K

H

T

L

H

G

G

I

I

T

K

F

G

A

L

A

P

S

S

I

V

L

V

N

N

F

V

V

V

V

I

L

L

T

I

A

A

S

V

L

L

S

S

A

V

I

G

N

N

S

S

D

A

V

I

F

Y

G

A

V

C

G

S

R

R

M

T

L

M

H

V

G

A

M

L

A

A

E

E

Q

Q

G

R

S

F

A

L

P

P

K

E

M

I

F

F

A

S

K

Y

T

V

S

D

R

R

R

K

G

G

V

R

P

P

W

L

V

V

T

G

V

I

M

A

V

V

M

T

T

S

I

A

A

F

L

G

L

L

F

I

A

A

V

F

Y

V

L

A

N

A

Y

S

I

K

M

K

P

E

E

G

N

E

V

V

F

F

L

N

V

W

I

L

A

L

S

A

L

L

A

S

T

G

F

L

A

S

T

S

V

L

W

F

V

T

W

W

I

G

M

G

I

I

L

C

L

I

S

C

Q

H

I

I

A

R

F

F

R

R

R

K

R

A

L

L

P

A

E

Q

E

G

A

R

-

G

-

L

K

D

A

E

L

L

K

S

F

F

K

K

V

S

P

P

G

T

G

G

V

V

T

W

T

G

T

S

V

Y

I

W

G

G

L

-

L

L

F

F

L

M

V

V

F

I

I

I

I

M

G

F

L

I

-

A

-

Q

-

F

-

Y

-

V

-

A

-

V

-

F

-

P

I

V

G

G

Y

D

H

S

P

P

D

S

T

A

R

E

-

G

-

F

I

F

S

E

L

A

Y

Y

V

L

G

S

F

F

A

P

W

L

I

V

I

M

L

V

L

M

L

Y

I

I

G

G

W

H

Q

K

F

I

K

Y

T

K

R

R

R

N

A297 (≠ G213) mutation to V: Impairs basic amino-acids transport and regulation by these amino-acids.

Sites not aligning to the query:

76 modified: Glycyl lysine isopeptide (Lys-Gly) (interchain with G-Cter in ubiquitin)

P48813 High-affinity glutamine permease from Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast) (see paper)
28% identity, 84% coverage: 2:386/456 of query aligns to 140:537/663 of P48813

query
sites
P48813

E

Q

S

K

S

Q

N

E

K

N

L

L

K

K

R

K

G

S

L

I

S

K

T

P

R

R

H

H

I

T

R

V

F

M

M

M

A

S

L

L

G

G

S

T

A

G

I

I

G

G

T

T

G

G

L

L

F

L

Y

V

G

G

S

N

A

S

D

K

A

V

I

L

K

N

M

N

A

A

G

G

P

P

S

G

-

G

V

L

L

I

A

G

Y

Y

I

A

I

I

G

M

G

G

V

S

A

C

A

V

Y

Y

I

C

I

I

M

I

R

Q

A

A

L

C

G

G

E

E

M

L

S

A

V

V

-

I

H

Y

N

S

P

D

A

L

A

I

S

G

F

F

S

N

R

T

Y

Y

A

P

Q

L

D

F

Y

L

L

V

G

D

P

P

L

A

A

L

G

G

Y

F

I

S

T

V

G

A

W

W

T

L

Y

F

C

C

F

L

E

Q

I

W

L

L

I

C

V

V

A

C

I

P

A

L

D

E

V

L

T

V

A

T

F

A

G

S

I

M

Y

T

M

I

G

K

V

Y

W

W

F

T

P

T

A

S

V

V

P

N

H

P

W

D

V

V

W

F

V

V

L

V

S

I

V

F

V

Y

L

V

I

L

I

I

C

V

A

V

V

I

N

N

L

V

M

F

S

G

V

A

K

K

V

G

F

Y

G

A

E

E

L

A

E

D

F

F

W

F

F

F

S

N

F

C

K

K

V

-

A

-

T

-

I

-

I

I

M

L

I

M

L

I

A

V

G

G

F

F

G

F

I

I

I

L

I

A

W

I

G

I

I

I

G

D

N

C

G

G

Q

A

P

G

T

T

G

D

I

G

H

Y

-

I

-

G

-

S

N

K

L

Y

W

W

S

R

N

D

G

P

G

G

F

A

F

F

S

R

N

G

G

D

-

T

-

P

-

I

-

Q

-

R

W

F

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K

G

G

M

V

V

V

M

A

S

T

L

F

Q

V

M

T

V

A

M

A

F

F

A

A

Y

F

G

G

G

M

I

S

E

E

I

Q

I

L

G

A

I

M

T

T

A

A

G

S

E

E

A

Q

K

S

D

N

P

P

E

R

K

K

S

A

I

I

P

P

R

S

A

A

I

A

N

K

S

K

V

M

P

I

M

Y

R

R

I

I

L

L

V

F

F

V

Y

F

V

L

G

A

T

S

L

L

F

T

V

L

I

V

M

G

S

F

I

L

Y

V

P

P

W

Y

-

T

-

S

N

D

Q

Q

V

L

-

L

-

G

-

A

-

G

-

S

G

A

T

T

N

K

G

A

S

S

P

P

F

Y

V

V

L

I

T

A

F

V

Q

S

H

S

L

H

G

G

I

V

T

R

F

V

A

V

A

P

S

H

I

F

L

I

N

N

F

A

V

V

V

I

L

L

T

L

A

S

S

V

L

L

S

S

A

V

I

A

N

N

S

G

D

A

V

F

F

Y

G

T

V

S

G

S

R

R

M

I

L

L

H

M

G

S

M

L

A

A

E

K

Q

Q

G

G

S

N

A

A

P

P

K

K

M

C

F

F

A

D

K

Y

T

I

S

D

R

R

R

E

G

G

V

R

P

P

W

A

V

A

T

A

V

M

M

L

V

V

M

S

T

A

I

L

A

F

L

G

L

V

F

I

A

A

V

F

Y

C

L

A

N

S

Y

S

I

K

M

K

P

E

E

E

N

D

V

V

F

F

L

T

V

W

I

L

A

L

S

A

L

I

A

S

T

G

F

L

A

S

T

Q

V

L

W

F

V

T

W

W

I

I

M

T

I

I

L

C

L

L

S

S

Q

H

I

I

A

R

F

F

R

R

R

A

L

M

Sites not aligning to the query:

132 modified: Glycyl lysine isopeptide (Lys-Gly) (interchain with G-Cter in ubiquitin)

Q03770 SPS-sensor component SSY1; Amino-acid permease homolog SSY1 from Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast) (see paper)
24% identity, 91% coverage: 7:421/456 of query aligns to 277:772/852 of Q03770

query
sites
Q03770

L

I

K

Q

R

R

G

K

L

L

S

K

T

V

R

R

H

H

I

I

R

Q

F

M

M

L

A

S

L

I

G

G

S

A

A

C

I

F

G

S

T

V

G

G

L

L

F

F

Y

L

G

T

S

S

A

G

D

K

A

A

I

F

K

S

M

I

A

A

G

G

P

P

-

F

S

G

V

T

L

L

A

G

Y

F

I

G

I

L

G

T

G

G

V

S

A

I

Y

L

I

A

I

T

M

M

R

L

A

S

L

F

G

T

E

E

M

L

S

S

V

T

H

L

N

I

P

P

A

V

A

S

S

S

S

G

F

F

S

S

R

G

Y

L

A

A

Q

S

D

R

Y

F

L

V

G

E

P

D

L

A

A

F

G

G

Y

F

I

A

T

L

G

G

W

W

T

T

Y

Y

C

W

F

I

E

S

I

C

L

M

I

L

V

A

A

L

I

P

A

A

D

Q

V

V

T

S

A

S

F

S

G
x
T

I

F

Y

Y

M

L

G

S

V

Y

W

Y

F

N

P

N

-

V

-

N

-

I

-

S

-

K

-

G

A

V

V

T

P

A

H

G

W

F

V

I

W

T

V

L

L

F

S

S

V

A

V

F

L

S

I

I

I

V

C

-

A

-

V

V

N

N

L

L

M

L

S

D

V

V

K

S

V

I

F

M

G

G

E

E

L

I

E

V

F

Y

W

-

F

-

S

-

F

V

F

A

K

G

V

I

A

S

T

K

I

V

I

I

M

A

I

I

L

L

A

M

G

V

F

F

G

T

I

M

I

I

W

L

G

N

I

A

G

G

N

H

G

G

G

N

Q

D

P

-

T

-

G

-

I

I

H

H

N

E

-

G

-

V

-

G

-

F

-

R

L

Y

W

W

S

D

N

S

G

S

G

K

F

S

F

V

S

R

N

N

-

L

-

T

-

Y

-

G

-

L

-

Y

-

R

-

P

-

T

-

F

-

D

-

L

-

A

-

D

-

A

-

G

-

E

-

G

-

S

-

K

-

G

-

I

-

S

G

G

W

P

L

K

G

G

M

R

V

F

M

L

S

A

L

T

Q

A

M

S

V

V

M

M

-

L

-

I

-

S

-

T

F

F

A

A

Y

F

G

S

G

G

I

V

E

E

I

M

I

T

G

F

I

L

T

A

A

S

G

G

E

E

A

A

K

I

D

N

P

P

E

R

K

K

S

T

I

I

P

P

R

S

A

A

I

T

N

K

S

R

V

T

P

F

M

S

R

I

I

V

L

L

V

I

F

S

Y

Y

V

V

G

F

T

L

L

I

F

F

V

S

I

V

-

G

-

I

-

N

-

I

-

Y

-

S

-

G

-

D

-

P

-

R

-

L

M

L

S

S

I

Y

Y

F

P

P

-

G

-

I

-

S

-

E

-

K

-

R

-

Y

-

E

-

A

-

I

-

K

-

G

-

T

-

G

-

M

-

D

W

W

N

R

-

L

-

R

-

T

-

N

-

C

-

R

-

G

-

I

-

D

-

Y

-

R

-

Q

-

I

Q

S

V

V

G

G

T

T

N

G

-

Y

G

S

S

S

P

P

F

W

V

V

L

V

T

A

F

L

Q

Q

H

N

L

F

G

G

I

L

T

C

F

T

A

F

A

A

S

S

I

A

L

F

N

N

F

A

V

I

V

L

L

I

T

F

A

F

S

T

L

A

S

T

A

A

I

G

N

I

S

S

D

S

V

L

F

F

G

S

V

C

G

S

R

R

M

T

L

L

H

Y

G

A

M

M

A

S

E

V

Q

Q

G

R

S

K

A

A

P

P

K

P

M

V

F

F

A

E

K

I

T

C

S

S

R

K

R

R

G

G

V

V

P

P

W

Y

V

V

T

S

V

V

M

I

V

F

M

S

T

S

I

L

-

F

A

S

L

V

L

I

F

A

A

Y

V

I

Y

A

L

V

N

D

Y

Q

I

T

M

A

P

I

E

E

N

N

V

-

F

F

L

D

V

V

I

L

A

A

S

N

L

V

A

S

T

S

F

A

A

S

T

T

V

S

W

I

V

I

W

W

I

M

M

G

I

L

L

N

L

L

S

S

Q

F

I

L

A

R

F

F

-

Y

-

A

-

L

-

K

-

Q

R

R

R

K

R

D

L

I

P

I

P

S

E

R

E

N

A

D

K

S

A

S

L

Y

K

P

F

Y

K

K

V

S

P

P

G

F

G

Q

V

P

T

Y

T

L

T

A

V

I

I

Y

G

G

L

L

L

V

F

G

L

C

V

S

F

L

I

F

I

V

G

I

L

F

I

M

G

G

Y

Y

T382 (≠ G111) mutation T->H,L: Constitutively active, up-regulates amino acid permease transcription in response to subthreshold concentrations of amino acids.; mutation to K: In SSY1-102; constitutively active, up-regulates amino acid permease transcription in the absence of amino-acids.; mutation to R: Constitutively active, up-regulates amino acid permease transcription in the absence of amino acids.

P76037 Putrescine importer PuuP from Escherichia coli (strain K12) (see paper)
27% identity, 38% coverage: 63:236/456 of query aligns to 74:242/461 of P76037

query
sites
P76037

G

G

E

K

M

L

S

V

V

R

H

Q

N

F

P

P

A

E

A

A

S

G

S

S

F

A

S

Y

R

T

Y

Y

A

A

Q

Q

D

K

Y

S

L

I

G

N

P

P

L

H

A

V

G

G

Y

F

I

M

T

V

G

G

W

W

T

S

Y

S

C

L

F

L

E

D

I
x
Y

L

L

I

F

V

L

A

P

I

M

A

I

D

N

V

V

T

L

A

L

F

A

G

K

I

I

Y

Y

M

L

G

S

V

A

W

L

F

F

P

P

A

E

V

V

P

P

H

P

W

W

V

V

W

W

V

V

L

V

S

T

V

F

V

V

L

A

I

I

I

L

C

T

A

A

V

A

N

N

L

L

M

K

S

S

V

V

K

N

V

L

F

V

G

A

E

N

L

F

E

N

F

T

W

L

F

F

S

V

F

L

F

V

K

Q

V

I

A

S

T

I

I

M

I

V

M

F

I

I

L

F

A

-

G

-

F

-

G

-

I

L

I

V

W

Q

G

G

I

L

G

H

N

K

G

G

G

-

Q

-

P

-

T

E

G

G

I

V

H

G

N

T

L

V

W

W

S

S

N

L

G

Q

G

P

F

F

F

I

S

S

N

E

G

N

-

A

-

H

W

L

L

I

G

P

M

I

V

I

M

T

S

G

L

A

Q

T

M

I

V

V

M

C

F

F

A

S

Y

F

G

L

G

G

I

F

E

D

I

A

I

V

G

T

I

T

T

L

A

S

G

E

E

E

A

T

K

P

D

D

P

A

E

A

K

R

S

V

I

I

P

P

R

K

A

A

I

I

Y110 (≠ I99) mutation to X: The uptake activity is reduced to one-eighth of that of wild-type.

O34739 Serine/threonine exchanger SteT from Bacillus subtilis (strain 168) (see paper)
22% identity, 85% coverage: 3:390/456 of query aligns to 4:381/438 of O34739

query
sites
O34739

S

E

S

D

N

N

K

G

L

L

K

K

R

K

G

E

L

I

S

G

T

L

R

L

H

F

I

A

R

L

F

T

M

L

A

V

L

I

G

G

S

T

A

I

I

I

G

G

T

S

G

G

L

V

F

F

Y

M

G

K

S

P

A

G

D

A

A

V

I

L

K

A

M

Y

A

S

G

G

P

D

S

S

-

K

-

M

V

A

L

L

L

F

A

A

Y

W

I

L

G

G

V

I

A

L

A

T

Y

L

I

A

I

G

M

G

R

L

A

T

L

I

G

A

E

E

M

I

S

G

V

T

H

Q

N

I

P

P

A

K

A

T

S

G

F

L

S

Y

R

T

Y

Y

A

L

Q

E

D

E

Y

V

L

Y

G

G

P

E

L

F

A

W

G

G

Y

F

I

L

T
x
C

G

G

W

W

T

V

Y

-

C

-

F

-

E

Q

I

I

L

I

I

I

V

Y

A

G

I

P

A

A

D

I

V

I

T

G

A

A

F

L

G

G

I

L

Y

Y

M

F

G

G

V

S

W

L

F

M

P

A

A

N

V

L

P

F

H

G

W

W

V

G

W

S

V

G

L

L

S

S

-

K

-

V

-

I

-

G

-

I

V

A

V

V

L

L

I

F

I

L

C
|
C

A

V

V

I

N

N

L

I

M

I

S

G

V

T

K

K

V

Y

F

G

G

G

E

-

L

-

E

-

F

-

W

-

F

-

S

-

F

F

F

V

K

Q

V

T

A

L

T

T

I

T

I

I

I

G

M

K

I

L

L

I

A

P

G

I

F

A

G
x
C

I

I

I

V

W

F

G

G

I

L

G

W

N

K

G

G

G

D

Q

Q

P

H

-

I

-

F

T

T

G

A

I

V

H

N

N

E

L

S

W

I

S

S

N

D

G

M

G

N

F

F

F

G

S

A

N

-

G

-

W

-

L

-

G

-

M

-

V

-

M

-

S

A

L

I

Q

L

M

A

V

T

M

L

F

F

A

A

Y

Y

G

D

G

G

I

W

E

I

I

L

G

A

I

A

T

L

A

G

G

G

E

E

A

M

K

K

D

N

P

P

E

E

K

K

S

L

I

L

P

P

R

R

A

A

I

M

N

T

S

G

V

G

P

L

M

L

R

I

I

V

L

T

V

A

F

I

Y

Y

V

I

G

F

T

I

L

N

F

F

V

A

I

L

M

L

S

H

I

I

Y

L

P

S

W

A

N

N

Q

E

V

I

G

V

T

T

N

L

G

G

S

E

P

N

F

A

V

T

L

S

T

T

F

A

Q

A

H

T

L

M

G

L

I

F

-

G

T

S

F

I

A

G

S

K

I

L

L

I

N

S

F

V

V

G

V

I

L

I

T

V

A

S

S

I

L

F

S

G

A
x
C

I

L

N

N

S

G

D

K

V

V

F

L

G

S

V

F

G

P

R

R

M

V

L

S

H

F

G

A

M

M

A

A

E

E

Q

R

G

K

S

Q

A

L

P

P

-

F

-

A

-

E

K

K

M

L

F

S

A

H

K

V

T

H

S

P

R

S

R

F

G

R

V

T

P

P

W

W

V

-

T

-

V

-

M

-

V

-

M

-

T

-

I

I

A

A

L

I

L

S

F

F

A

Q

V

I

Y

A

L

L

N

A

Y

L

I

I

M

M

-

M

-

L

-

I

-

S

-

N

P

P

E

D

N

K

V

-

F

-

L

-

V

-

I

L

A

S

S

E

L

I

A

S

T

I

F

F

A

M

T

I

V

Y

W

I

V

F

W

Y

I

V

M

M

I

A

L

F

S

A

Q

V

I

F

A

I

F

L

R

R

R

K

R

R

L

A

P

K

P

G

E

E

E

K

C94 (≠ T91) mutation to S: Retains 25% of the transport activity; when associated with S-141; S-168; S-291 and S-415.
C141 (= C135) mutation to S: Retains 25% of the transport activity; when associated with S-94; S-168; S-291 and S-415.
C168 (≠ G169) mutation to S: Retains 25% of the transport activity; when associated with S-94; S-141; S-291 and S-415.
C291 (≠ A297) mutation to S: Retains 25% of the transport activity; when associated with S-94; S-141; S-168 and S-415.

Sites not aligning to the query:

415 C→S: Retains 25% of the transport activity; when associated with S-94; S-141; S-168 and S-291.

P30825 High affinity cationic amino acid transporter 1; CAT-1; CAT1; Ecotropic retroviral leukemia receptor homolog; Ecotropic retrovirus receptor homolog; Solute carrier family 7 member 1; System Y+ basic amino acid transporter from Homo sapiens (Human) (see paper)
24% identity, 72% coverage: 2:328/456 of query aligns to 23:381/629 of P30825

query
sites
P30825

E

R

S

E

N

T

K

R

L

L

K

S

R

R

G

C

L

L

S

N

T

T

R

F

H

D

I

L

R

V

F

A

M

L

A

G

L

V

G

G

S

S

A

T

I

L

G

G

T

A

G

G

L

V

F

Y

-

V

Y

L

G

A

S

G

A

A

D

V

A

A

I

R

K

E

M

N

A

A

G

G

P

P

S

A

V

I

L

V

L

I

A

S

Y

F

I

L

I

I

G

A

V

L

A

A

A

S

Y

V

I

L

I

A

M

G

R

L

A

C

L

Y

G

G

E

E

M

F

S

G

V

A

H

R

N

V

P

P

A

K

A

T

S

G

S

S

F

A

S

Y

R

L

Y

Y

A

S

Q

Y

D

V

Y

T

L

V

G

G

P

E

L

L

A

W

G

A

Y

F

I

I

T

T

G

G

W

W

T

N

Y

L

C

I

F

L

E

S

I

Y

L

I

I

I

-

G

-

T

-

S

V

V

A

A

I

R

A

A

D

W

V

S

T

A

A

T

F

F

G

D

-

E

-

L

-

I

-

G

-

R

-

P

-

I

-

G

-

E

-

F

-

S

-

R

I

T

Y

H

M

M

G

T