SitesBLAST

L334 (= L255) mutation to W: Ability to transport lactose in a saturable manner.
L372 (= L294) mutation to W: Growth on maltose but not on media containing either maltoheptaose or maltoheptaose plus maltose.
N376 (≠ Q298) mutation N->K,H: No growth on maltose.
G380 (= G302) mutation G->C,S: No growth on maltose.
E401 (= E323) mutation E->A,C,K,L: Reduction of transport rate.
S403 (≠ A325) mutation S->C,D,K,L: Reduction of transport rate.
G407 (= G329) mutation G->A,P: No effect.
P420 (= P342) mutation to A: No effect.

4ki0F Crystal structure of the maltose-binding protein/maltose transporter complex in an outward-facing conformation bound to maltohexaose (see paper)
43% identity, 55% coverage: 180:420/435 of query aligns to 244:488/490 of 4ki0F

query
sites
4ki0F

V

T

G

G

F

W

K

K

N

N

F

F

I

T

N

R

I

V

F

F

T

T

L

D

S

E

I

G

W

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D

A

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W

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W

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A

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C

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M

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A

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C

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D

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M

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I

L

L

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P

W
x
Y

A

A

V

V

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P

G

S

F

F

V

I

T

S

I

I

L

L

V

I

F

F

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K

G

G

M

L

F

F

N

N

D

Q

S

S

F

F

G

G

V

E

I

I

N

N

N

M

A

M

I

L

L

S

S

A

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L

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F

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G

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P

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-

A

A

W

W

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T

T

A

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W

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G

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M

A

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C

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A

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D

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Y

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E

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A

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M

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D

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N

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L

L

F

L

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N

N

G

G

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P

A

D

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R

A

L

G

G

S

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N

T

-

T

-

P

A

A

G

G

G

Y

T

T

D

D

I

L

L

L

V

V

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N

W

Y

I

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Y

K

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L

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A

M

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E

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S

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A

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A

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T

L

L

L

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S

F

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F

L

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V

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G

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L

L

A

A

L

I

binding alpha-D-glucopyranose: Y310 (≠ W246), N361 (≠ Q298), G365 (= G302), Y368 (≠ F305), S418 (≠ Q355), N422 (= N359)

P94529 Arabinooligosaccharides transport system permease protein AraP from Bacillus subtilis (strain 168) (see paper)
25% identity, 68% coverage: 136:432/435 of query aligns to 32:312/313 of P94529

query
sites
P94529

P

P

Y

Y

L

L

M

F

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A

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P

G

F

F

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L

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I

L

I

F

S

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V

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F

A

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M

A

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F

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N

-

Y

-

N

-

L

-

Y

-

H

R

T

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L

P

P

A

G

K

E

L

-

V

V

D

S

W

F

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V

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G

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S

N

N

F

Y

I

T

N

-

I

-

F

-

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-

L

-

S

A

I

L

W

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R

N

S

P

T

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F

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D

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W

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L

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F

L

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G

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E

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D

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M

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F

N

E

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E

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Y

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N

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N

N

G

N

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P

A

G

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A

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G

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N

A

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G

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E

E205 (≠ D320) mutation to A: Has no effect on the growth rate.
E208 (= E323) mutation to A: Small negative impact in the uptake of arabinotriose. Shows an increase of about 10% in the doubling time.
D213 (= D328) mutation to A: Severely impairs the uptake of arabinotriose. Shows a 3.6-fold increase of the doubling time.

7cagA Mycobacterium smegmatis lpqy-sugabc complex in the catalytic intermediate state (see paper)
24% identity, 54% coverage: 139:372/435 of query aligns to 13:233/285 of 7cagA

query
sites
7cagA

M

L

I

I

T

A

P

P

G

A

F

V

I

L

L

L

L

M

V

L

F

A

V

V

V

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A

F

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P

I

I

L

G

F

Y

G

A

F

V

A

W

I

L

A

S

F

L

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Q

N

R

Y

Y

N

N

L

L

Y

A

H

E

T

P

P

-

A

-

K

H

L

D

V

T

D

E

W

F

V

I

G

G

F

L

K

A

N

N

F

Y

I

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N

T

I

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F

L

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T

L

D

S

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I

Y

W

W

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A

F

F

F

A

D

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V

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L

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G

W

I

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V

W

-

T

-

L

-

A

S

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V

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A

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C

F

T

A

V

L

G

G

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L

A

L

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A

I

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L

L

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F

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A

F

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I

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L

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Y

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F

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V

A

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A

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Y

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Y

M

A

F

W

N

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D

P

S

G

F

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G

G

V

Y

I

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N

A

N

N

A

L

I

L

L

P

S

E

F

-

G

G

I

S

S

A

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P

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-

A

-

W

-

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L

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D

D

P

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L

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A

A

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A

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x
E

T

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W

W

L
x
K

G

T

F

T

P

P

F

F

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M

F

A

A

L

M

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L

G

A

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G

L

L

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A

A

L

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P

D

Q

D

D

L

L

Y

L

E

N

A

A

T

Q

M

V

D

D

G

G

A

A

S

G

A

P

F

W

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K

R

R

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L

R

T

T

K

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V

T

I

L

L

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P

L

M

V

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K

Y

P

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A

I

I

A

L

P

V

I

A

I

L

T

F

Q
x
R

Y

T

T

L

F
x
D

N

A

F

F

N

R

N

I

F

F

N

D

I

N

I

I

Y

Y

L

I

F

L

N

T

N

G

G

G

binding alpha-D-glucopyranose: E159 (≠ Q298), K162 (≠ L301), R216 (≠ Q355), D219 (≠ F358)

Query Sequence

>BWI76_RS06700 FitnessBrowser__Koxy:BWI76_RS06700
MIINPSENIAEARGAGRHAWCGLLLAIVPGFGQFYHRQWLKGIVFLVLLSSFMSIFYDFL
SEGLWGLYTLGEEVPRDNSIFLLAEGIISVLIIAFGVLVYFLSLRDAWVNGKKRDEGVAL
NSVRKQYQMLLSEGFPYLMITPGFILLVFVVIFPILFGFAIAFTNYNLYHTPPAKLVDWV
GFKNFINIFTLSIWRSTFFDVLQWTVVWTLLATTLQCTVGVLLAILVNQKDLRFKPMIRT
IFILPWAVPGFVTILVFAGMFNDSFGVINNAILSFFGISPKAWLTDPFWTKTALIMMQTW
LGFPFVFAMTTGVLQAIPDDLYEAATMDGASAFTRLRTITLPLVLYSIAPIIITQYTFNF
NNFNIIYLFNNGGPAVAGSNAGGTDILVSWIYKLTMSSSQYAIAATITILLSIFVVGLAL
WQFRATKSFKNDDMA

Or try a new SitesBLAST search

SitesBLAST's Database

SitesBLAST's database includes (1) SwissProt entries with experimentally-supported functional features; and (2) protein structures with bound ligands, from the BioLip database.

by Morgan Price, Arkin group
Lawrence Berkeley National Laboratory