SitesBLAST

SitesBLAST – Find functional sites

SitesBLAST

Comparing BWI76_RS07720 BWI76_RS07720 iron-enterobactin transporter ATP-binding protein to proteins with known functional sites using BLASTp with E ≤ 0.001.

Or try Sites on a Tree, PaperBLAST, Conserved Domains, or compare to all protein structures

Found 20 (the maximum) hits to proteins with known functional sites (download)

P07821 Iron(3+)-hydroxamate import ATP-binding protein FhuC; Ferric hydroxamate uptake protein C; Ferrichrome transport ATP-binding protein FhuC; Iron(III)-hydroxamate import ATP-binding protein FhuC; EC 7.2.2.16 from Escherichia coli (strain K12) (see 2 papers)
45% identity, 90% coverage: 20:257/264 of query aligns to 24:261/265 of P07821

query
sites
P07821

K

R

T

T

I

L

A

L

E

H

S

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L

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N

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T

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I

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P

D

A

G

G

H

K

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T

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L

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I

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G

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H

N

N

G

G

C

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G

G

K
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K

S

S

T

T

L

L

R

K

T

M

L

L

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S

E

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F

A

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N

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P

A

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E

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E

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L

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A

A

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E

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V

K50 (= K46) mutation to Q: Lack of activity.
D172 (= D168) mutation to E: Lack of activity.
E173 (= E169) mutation to A: Lack of activity.

4u00A Crystal structure of ttha1159 in complex with adp (see paper)
35% identity, 82% coverage: 17:233/264 of query aligns to 12:225/241 of 4u00A

query
sites
4u00A

Y
x
F

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G

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x
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x
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G

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S
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S

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T

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-

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T

binding adenosine-5'-diphosphate: F12 (≠ Y17), V17 (≠ I22), S37 (≠ N42), G38 (= G43), S39 (≠ C44), G40 (= G45), K41 (= K46), S42 (= S47), T43 (= T48)

4ymuJ Crystal structure of an amino acid abc transporter complex with arginines and atps (see paper)
31% identity, 81% coverage: 17:231/264 of query aligns to 11:223/240 of 4ymuJ

query
sites
4ymuJ

Y
x
F

G

G

K

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L

T

E

I
x
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A

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E

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N

D

K

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G

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E

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I

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G

P

P

N
x
S

G
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G

C
x
S

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G

K
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K

S
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S

T
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T

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M

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D

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-

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W

N

R

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E

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A

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E
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E

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H

D

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E

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I

binding adenosine-5'-triphosphate: F11 (≠ Y17), V16 (≠ I22), S36 (≠ N42), G37 (= G43), S38 (≠ C44), G39 (= G45), K40 (= K46), S41 (= S47), T42 (= T48), E162 (= E169), H194 (= H202)
binding magnesium ion: S41 (= S47), E162 (= E169)

1f3oA Crystal structure of mj0796 atp-binding cassette (see paper)
33% identity, 77% coverage: 24:225/264 of query aligns to 22:226/232 of 1f3oA

query
sites
1f3oA

E

K

S

N

L

V

N

N

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T

N

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I

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K

D

E

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G

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N

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G

C

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K
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S
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S

T
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T

L

M

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A

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E

binding adenosine-5'-diphosphate: G41 (= G43), G43 (= G45), K44 (= K46), S45 (= S47), T46 (= T48)

Sites not aligning to the query:

binding adenosine-5'-diphosphate: 11

Q5M243 Energy-coupling factor transporter ATP-binding protein EcfA1; ECF transporter A component EcfA1; EC 7.-.-.- from Streptococcus thermophilus (strain ATCC BAA-250 / LMG 18311) (see paper)
28% identity, 86% coverage: 12:238/264 of query aligns to 6:233/276 of Q5M243

query
sites
Q5M243

Q

N

L

L

T

K

L

F

A

K

Y

Y

G

N

K

Q

K

D

T

Q

I

T

A

S

E

Y

S

T

L

L

N

N

-

D

-

V

-

S

V

F

T

H

I

V

P

K

D

H

G

G

H

E

F

W

T

L

A

S

I

I

I

V

G

G

P

H

N

N

G

G

C

S

G

G

K

K

S

S

T

T

L

T

L

A

R

R

T

L

L

I

S

G

R

G

L

L

M

L

T

V

P

A

A

D

S

S

G

G

H

Q

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I

Y

I

L

V

D

D

G

G

E

Q

Q

E

I

L

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E

Y

E

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W

E

D

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I

A

R

K

D

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K

I

I

G

G

L

M

L

V

A

F

Q

Q

N

N

A

P

T

D

T

N

P
x
Q

G

F

-

V

D

G

I

A

T

T

V

V

Q

E

E

D

L

D

V

V

A

A

R

F

G

G

-

L

-

E

-

N

-

K

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Y

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E

D

Q90 (≠ P93) mutation to A: No effect on ATPase, 10-fold decrease in riboflavin uptake; when associated with A-97 in EcfA2.
E163 (= E169) mutation to Q: 10-fold decrease in ATPase and riboflavin uptake; when associated with Q-171 in EcfA2.

1l2tA Dimeric structure of mj0796, a bacterial abc transporter cassette (see paper)
33% identity, 77% coverage: 24:225/264 of query aligns to 22:226/230 of 1l2tA

query
sites
1l2tA

E

K

S

N

L

V

N

N

V

L

T

N

I

I

P

K

D

E

G

G

H

E

F

F

T

V

A

S

I

I

I

M

G

G

P

P

N
x
S

G
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G

C
x
S

G
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G

K
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K

S
|
S

T
|
T

L

M

L

L

R

N

T

I

L

I

S

G

R

C

L

L

M

D

T

K

P

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A

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E

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G

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V

Y

Y

L

I

D

D

G

N

E

I

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K

I

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Q

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R

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Y

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A

D

S

D

K

D

E

E

V

L

A

T

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K

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I

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R

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D

R

K

I

I

G

G

L

F

L

V

A

F

Q

Q

N

Q

A

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T

L

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D

L

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-

R

-

G

-

R

-

Y

-

P

-

H

E

Q

L

P

P

L

L

F

I

T

F

R

K

W

Y

R

R

-

G

-

A

K

M

E

S

D

G

E

E

A

R

V

R

S

K

R

R

A

A

M

L

K

E

A

C

T

L

G

K

I

M

T

A

D

E

L

L

-

E

-

R

-
x
F

A

A

R

N

Q

H

S

K

V

P

D

N

T
x
Q

L

L

S
|
S

G

G

G
|
G

Q
|
Q

R

Q

Q

Q

R

R

A

V

W

A

I

I

A

A

M

R

V

A

L

L

A

A

Q

N

E

N

T

P

A

P

I

I

M

I

L

L

L

A

D

D

E

Q

P

P

T

T

T

G

W

A

L

L

D

D

I

S

S

K

H

T

Q

G

I

E

D

K

L

I

L

M

E

Q

L

L

S

K

E

K

L

L

N

N

Q

E

K

D

G

G

Y

K

T

T

L

V

A

V

V

V

L

T

H
|
H

D

D

L

I

N

N

Q

V

A

A

C

-

R

R

Y

F

A

G

T

E

H

R

L

I

I

I

A

Y

L

L

R

K

E

D

G

G

K

E

I

V

V

E

A

R

E

E

binding adenosine-5'-triphosphate: S40 (≠ N42), G41 (= G43), S42 (≠ C44), G43 (= G45), K44 (= K46), S45 (= S47), T46 (= T48), F138 (vs. gap), Q145 (≠ T143), S147 (= S145), G149 (= G147), Q150 (= Q148), H204 (= H202)

Sites not aligning to the query:

binding adenosine-5'-triphosphate: 11

Q5M244 Energy-coupling factor transporter ATP-binding protein EcfA2; ECF transporter A component EcfA2; EC 3.6.3.- from Streptococcus thermophilus (strain ATCC BAA-250 / LMG 18311) (see paper)
35% identity, 83% coverage: 26:243/264 of query aligns to 26:246/280 of Q5M244

query
sites
Q5M244

L

M

N

T

V

V

T

T

I

I

P

K

D

D

G

G

H

S

F

Y

T

T

A

A

I

F

I

I

G

G

P

H

N

T

G

G

C

S

G

G

K

K

S

S

T

T

L

I

L

M

R

Q

T

L

L

L

S

N

R

G

L

L

M

Y

T

L

P

P

A

T

S

S

G

G

H

Q

V

V

Y

K

L

V

D

D

-

D

-

T

-

I

G

N

E

S

Q

Q

I

S

Q

K

R

N

Y

K

A

E

S

I

K

K

E

P

V

I

A

R

K

K

R

K

I

V

G

G

L

L

L

V

A

F

Q

Q

N

F

A

P

T

E

T

S

P
x
Q

-

L

G

F

D

A

I

E

T

T

V

V

Q

L

E

E

L

D

V

I

A

A

R

F

G

G

R

-

Y

-

P

P

H

Q

Q

N

P

-

L

-

F

F

T

G

R

V

W

S

R

K

K

E

E

E

D

A

E

E

E

Q

A

R

V

A

S

L

R

E

A

S

M

L

K

R

A

L

T

V

G

G

I

L

T

S

D

D

L

E

A

L

R

R

-

D

Q

Q

S

N

V

P

D

F

T

D

L

L

S

S

G

G

Q

Q

R

M

Q

R

R

R

A

V

W

A

I

I

A

A

M

G

V

I

L

L

A

A

Q

M

E

Q

T

P

A

D

I

I

M

L

L

V

L

L

D

D

E
|
E

P

P

T

T

T

A

W

G

L

L

D

D

I

P

S

Q

H

G

Q

R

I

K

D

E

L

L

L

M

E

S

L

L

L

F

S

K

E

Q

L

L

N

H

Q

-

Q

L

K

S

G

G

Y

I

T

T

L

I

A

V

A

L

V

V

L

T

H

H

D

L

L

M

N

D

Q

D

A

V

C

A

R

D

Y

Y

A

A

T

T

H

A

L

V

I

N

A

V

L

M

R

E

E

K

G

G

K

R

I

L

V

V

A

L

E

S

G

G

A

T

P

P

K

K

E

D

I

V

V

F

S

Q

-

K

-

V

A

A

E

F

L

L

I

K

E

E

K

K

I

Q

Y

L

G

G

L

V

Q97 (≠ P93) mutation to A: No effect on ATPase, 10-fold decrease in riboflavin uptake; when associated with A-90 in EcfA1.
E171 (= E169) mutation to Q: 10-fold decrease in ATPase and riboflavin uptake; when associated with Q-163 in EcfA1.

3c4jA Abc protein artp in complex with atp-gamma-s
31% identity, 84% coverage: 12:233/264 of query aligns to 8:227/242 of 3c4jA

query
sites
3c4jA

Q

Q

L

L

T

K

L

K

A

S

Y
x
F

G

G

K

S

K

L

T

E

I
x
V

A

L

E

K

S

G

L

I

N

N

V

V

T

H

I

I

P

R

D

E

G

G

H

E

F

V

T

V

A

V

I

V

I

I

G

G

P

P

N
x
S

G
|
G

C
x
S

G
|
G

K
|
K

S
|
S

T
|
T

L

F

L

L

R

R

T

C

L

L

S

N

R

L

L

L

M

E

T

D

P

F

A

D

S

E

G

G

H

E

V

I

Y

I

L

I

D

D

G

G

E

I

Q

N

I

L

Q

K

R

A

Y

K

A

D

S

T

K

N

-

L

-

N

E

K

V

V

A

R

K

E

R

E

I

V

G

G

L

M

L

V

A

F

Q

Q

-

R

-

F

-

N

-

L

-

F

-

P

N

H

A

M

T

T

T

V

P

L

G

N

D

N

I

I

T

T

V

L

Q

A

E

P

L

M

V

K

A

V

R

R

G

-

R

-

Y

-

P

-

H

-

Q

-

P

-

L

-

F

-

T

-

R

K

W

W

R

P

K

R

E

E

D

K

E

A

E

E

A

A

V

-

S

-

R

K

A

A

M

M

K

E

-

L

-

D

A

K

T

V

G

G

I

L

T

K

D

D

L

K

A

A

R

H

Q

A

S

Y

V

P

D

D

T

S

L

L

S

S

G

G

Q

Q

R

A

Q

Q

R

R

A

V

W

A

I

I

A

A

M

R

V

A

L

L

A

A

Q

M

E

E

T

P

A

K

I

I

M

M

L

L

L

F

D

D

E

E

P

P

T

T

T

S

W

A

L

L

D

D

I

P

S

E

H

M

Q

V

I

G

D

E

L

V

L

L

E

S

L

V

L

M

S

K

E

Q

L

L

N

A

Q

N

Q

E

K

-

G

G

Y

M

T

T

L

M

A

V

V

V

L

T

H

H

D

E

L

M

N

G

Q

F

A

A

C

R

R

E

Y

V

A

G

T

D

H

R

L

V

I

L

A

F

L

M

R

D

E

G

G

G

K

Y

I

I

V

I

A

E

E

E

G

G

A

K

P

P

K

E

E

D

I

L

V

F

S

D

binding phosphothiophosphoric acid-adenylate ester: F13 (≠ Y17), V18 (≠ I22), S38 (≠ N42), G39 (= G43), S40 (≠ C44), G41 (= G45), K42 (= K46), S43 (= S47), T44 (= T48)

3c41J Abc protein artp in complex with amp-pnp/mg2+
31% identity, 84% coverage: 12:233/264 of query aligns to 8:227/242 of 3c41J

query
sites
3c41J

Q

Q

L

L

T

K

L

K

A

S

Y
x
F

G

G

K

S

K

L

T

E

I
x
V

A

L

E

K

S

G

L

I

N

N

V

V

T

H

I

I

P

R

D

E

G

G

H

E

F

V

T

V

A

V

I

V

I

I

G

G

P

P

N
x
S

G
|
G

C

S

G
|
G

K
|
K

S
|
S

T
|
T

L

F

L

L

R

R

T

C

L

L

S

N

R

L

L

L

M

E

T

D

P

F

A

D

S

E

G

G

H

E

V

I

Y

I

L

I

D

D

G

G

E

I

Q

N

I

L

Q

K

R

A

Y

K

A

D

S

T

K

N

-

L

-

N

E

K

V

V

A

R

K

E

R

E

I

V

G

G

L

M

L

V

A

F

Q

Q

-

R

-

F

-

N

-

L

-

F

-

P

N

H

A

M

T

T

T

V

P

L

G

N

D

N

I

I

T

T

V

L

Q

A

E

P

L

M

V

K

A

V

R

R

G

-

R

-

Y

-

P

-

H

-

Q

-

P

-

L

-

F

-

T

-

R

K

W

W

R

P

K

R

E

E

D

K

E

A

E

E

A

A

V

-

S

-

R

K

A

A

M

M

K

E

-

L

-

D

A

K

T

V

G

G

I

L

T

K

D

D

L

K

A

A

R

H

Q

A

S

Y

V

P

D

D

T

S

L

L

S

S

G

G

Q

Q

R

A

Q

Q

R

R

A

V

W

A

I

I

A

A

M

R

V

A

L

L

A

A

Q

M

E

E

T

P

A

K

I

I

M

M

L

L

L

F

D
|
D

E

E

P

P

T

T

T

S

W

A

L

L

D

D

I

P

S

E

H

M

Q

V

I

G

D

E

L

V

L

L

E

S

L

V

L

M

S

K

E

Q

L

L

N

A

Q

N

Q

E

K

-

G

G

Y

M

T

T

L

M

A

V

V

V

L

T

H

H

D

E

L

M

N

G

Q

F

A

A

C

R

R

E

Y

V

A

G

T

D

H

R

L

V

I

L

A

F

L

M

R

D

E

G

G

G

K

Y

I

I

V

I

A

E

E

E

G

G

A

K

P

P

K

E

E

D

I

L

V

F

S

D

binding phosphoaminophosphonic acid-adenylate ester: F13 (≠ Y17), V18 (≠ I22), S38 (≠ N42), G39 (= G43), G41 (= G45), K42 (= K46), S43 (= S47), T44 (= T48)
binding magnesium ion: S43 (= S47), D163 (= D168)

2olkA Abc protein artp in complex with adp-beta-s
31% identity, 84% coverage: 12:233/264 of query aligns to 8:227/242 of 2olkA

query
sites
2olkA

Q

Q

L

L

T

K

L

K

A

S

Y
x
F

G

G

K

S

K

L

T

E

I
x
V

A

L

E

K

S

G

L

I

N

N

V

V

T

H

I

I

P

R

D

E

G

G

H

E

F

V

T

V

A

V

I

V

I

I

G

G

P

P

N
x
S

G
|
G

C
x
S

G
|
G

K
|
K

S
|
S

T
|
T

L

F

L

L

R

R

T

C

L

L

S

N

R

L

L

L

M

E

T

D

P

F

A

D

S

E

G

G

H

E

V

I

Y

I

L

I

D

D

G

G

E

I

Q

N

I

L

Q

K

R

A

Y

K

A

D

S

T

K

N

-

L

-

N

E

K

V

V

A

R

K

E

R

E

I

V

G

G

L

M

L

V

A

F

Q

Q

-

R

-

F

-

N

-

L

-

F

-

P

N

H

A

M

T

T

T

V

P

L

G

N

D

N

I

I

T

T

V

L

Q

A

E

P

L

M

V

K

A

V

R

R

G

-

R

-

Y

-

P

-

H

-

Q

-

P

-

L

-

F

-

T

-

R

K

W

W

R

P

K

R

E

E

D

K

E

A

E

E

A

A

V

-

S

-

R

K

A

A

M

M

K

E

-

L

-

D

A

K

T

V

G

G

I

L

T

K

D

D

L

K

A

A

R

H

Q

A

S

Y

V

P

D

D

T

S

L

L

S

S

G

G

Q

Q

R

A

Q

Q

R

R

A

V

W

A

I

I

A

A

M

R

V

A

L

L

A

A

Q

M

E

E

T

P

A

K

I

I

M

M

L

L

L

F

D

D

E

E

P

P

T

T

T

S

W

A

L

L

D

D

I

P

S

E

H

M

Q

V

I

G

D

E

L

V

L

L

E

S

L

V

L

M

S

K

E

Q

L

L

N

A

Q

N

Q

E

K

-

G

G

Y

M

T

T

L

M

A

V

V

V

L

T

H

H

D

E

L

M

N

G

Q

F

A

A

C

R

R

E

Y

V

A

G

T

D

H

R

L

V

I

L

A

F

L

M

R

D

E

G

G

G

K

Y

I

I

V

I

A

E

E

E

G

G

A

K

P

P

K

E

E

D

I

L

V

F

S

D

binding 5'-o-[(r)-hydroxy(thiophosphonooxy)phosphoryl]adenosine: F13 (≠ Y17), V18 (≠ I22), S38 (≠ N42), G39 (= G43), S40 (≠ C44), G41 (= G45), K42 (= K46), S43 (= S47), T44 (= T48)

2oljA Abc protein artp in complex with adp/mg2+
31% identity, 84% coverage: 12:233/264 of query aligns to 8:227/242 of 2oljA

query
sites
2oljA

Q

Q

L

L

T

K

L

K

A

S

Y
x
F

G

G

K

S

K

L

T

E

I
x
V

A

L

E

K

S

G

L

I

N

N

V

V

T

H

I

I

P

R

D

E

G

G

H

E

F

V

T

V

A

V

I

V

I

I

G

G

P

P

N
x
S

G
|
G

C
x
S

G
|
G

K
|
K

S
|
S

T
|
T

L

F

L

L

R

R

T

C

L

L

S

N

R

L

L

L

M

E

T

D

P

F

A

D

S

E

G

G

H

E

V

I

Y

I

L

I

D

D

G

G

E

I

Q

N

I

L

Q

K

R

A

Y

K

A

D

S

T

K

N

-

L

-

N

E

K

V

V

A

R

K

E

R

E

I

V

G

G

L

M

L

V

A

F

Q

Q

-

R

-

F

-

N

-

L

-

F

-

P

N

H

A

M

T

T

T

V

P

L

G

N

D

N

I

I

T

T

V

L

Q

A

E

P

L

M

V

K

A

V

R

R

G

-

R

-

Y

-

P

-

H

-

Q

-

P

-

L

-

F

-

T

-

R

K

W

W

R

P

K

R

E

E

D

K

E

A

E

E

A

A

V

-

S

-

R

K

A

A

M

M

K

E

-

L

-

D

A

K

T

V

G

G

I

L

T

K

D

D

L

K

A

A

R

H

Q

A

S

Y

V

P

D

D

T

S

L

L

S

S

G

G

Q

Q

R

A

Q

Q

R

R

A

V

W

A

I

I

A

A

M

R

V

A

L

L

A

A

Q

M

E

E

T

P

A

K

I

I

M

M

L

L

L

F

D

D

E

E

P

P

T

T

T

S

W

A

L

L

D

D

I

P

S

E

H

M

Q

V

I

G

D

E

L

V

L

L

E

S

L

V

L

M

S

K

E

Q

L

L

N

A

Q

N

Q

E

K

-

G

G

Y

M

T

T

L

M

A

V

V

V

L

T

H

H

D

E

L

M

N

G

Q

F

A

A

C

R

R

E

Y

V

A

G

T

D

H

R

L

V

I

L

A

F

L

M

R

D

E

G

G

G

K

Y

I

I

V

I

A

E

E

E

G

G

A

K

P

P

K

E

E

D

I

L

V

F

S

D

binding adenosine-5'-diphosphate: F13 (≠ Y17), V18 (≠ I22), S38 (≠ N42), G39 (= G43), S40 (≠ C44), G41 (= G45), K42 (= K46), S43 (= S47), T44 (= T48)

7chaI Cryo-em structure of p.Aeruginosa mlafebd with amppnp (see paper)
31% identity, 82% coverage: 13:228/264 of query aligns to 9:224/262 of 7chaI

query
sites
7chaI

L

L

T

T

L

F

A

K

Y
x
R

G

G

K

S

K

R

T

A

I

I

A

F

E

D

S

N

L

I

N

D

V

V

T

R

I

I

P

P

D

R

G

G

H

K

F

V

T

T

A

G

I

I

I

M

G

G

P

P

N
x
S

G
|
G

C
|
C

G
|
G

K
|
K

S
x
T

T
|
T

L

L

R

R

T

L

L

I

S

A

R

S

L

Q

M

L

T

R

P

P

A

S

S

K

G

G

H

E

V

V

Y

W

L

V

D

N

G

G

E

Q

-

N

-

L

-

P

Q

Q

I

L

Q

S

R

R

Y

G

A

D

S

L

K

F

E

D

V

M

A

R

K

K

R

Q

I

F

G

G

L

V

L

L

A

F

Q

Q

N

S

A

G

T

A

T

L

P

F

G

T

D

D

I

L

T

D

V

V

Q

F

E

E

L

N

V

V

A

A

R

F

G

-

R

-

Y

-

P

-

H

-

Q

-

P

P

L

L

F

R

T

V

R

H

W

T

R

Q

K

L

E

P

D

E

E

E

-

M

-

I

-

R

E

D

A

I

V

V

S

L

R

M

A

K

M

L

K

Q

A

A

T

V

G

G

I

L

T

R

D

G

L

A

A

V

R

E

Q

L

S

M

V

P

D

D

T

E

L

L

S

S

G

G

Q

M

R

K

Q

R

R

R

A

V

W

A

I

L

A

A

M

R

V

A

L

I

A

A

Q

L

E

D

T

P

A

Q

I

I

M

L

L

L

L

Y

D

D

E

E

P

P

T

F

T

V

W

G

L

Q

D

D

I

P

S

I

H

A

Q

M

I

G

D

V

L

L

L

V

E

R

L

L

L

I

S

R

E

L

L

L

N

N

Q

D

Q

A

K

L

G

G

Y

I

T

T

L

S

A

I

A

V

V

V

L

S

H

H

D

D

L

L

N

A

Q

E

A

T

C

A

R

S

Y

I

A

A

T

D

H

Y

L

I

I

Y

A

I

L

V

R

G

E

D

G

G

K

R

I

V

V

L

A

G

E

H

G

G

A

T

P

P

binding phosphoaminophosphonic acid-adenylate ester: R13 (≠ Y17), S38 (≠ N42), G39 (= G43), C40 (= C44), G41 (= G45), K42 (= K46), T43 (≠ S47), T44 (= T48)

5x40A Structure of a cbio dimer bound with amppcp (see paper)
34% identity, 94% coverage: 4:250/264 of query aligns to 1:246/280 of 5x40A

query
sites
5x40A

V

M

T

T

S

P

R

I

L

L

R

A

G

A

D

E

Q

A

L

L

T

T

L

Y

A

A

Y
x
F

-

P

G

G

K

G

K
x
V

T

K

I
x
A

A

L

E

D

S

D

L

L

N

S

V

L

T

A

I

V

P

P

D

K

G

G

H

E

F

S

T

L

A

A

I

I

I

L

G

G

P

P

N
|
N

G
|
G

C

A

G
|
G

K
|
K

S
|
S

T
|
T

L

L

R

L

T

H

L

L

S

N

R

G

L

T

M

L

T

R

P

P

A

Q

S

S

G

G

H

R

V

V

Y

L

L

L

D

G

G

G

E

T

Q

A

I

T

Q

G

R

-

Y

H

A

S

S

R

K

K

E

D

V

L

A

T

-

G

-

W

-

R

K

R

R

R

I

V

G

G

L

L

L

V

A

L

Q
|
Q

N

D

A

A

T

D

P

Q

-

L

G

F

D

A

I

T

T

T

V

V

Q

F

E

E

L

D

V

V

A

S

R

F

G

G

R

-

Y

-

P

-

H

-

Q

-

P

P

L

L

F

N

T

L

R

G

W

L

R

S

K

E

E

A

D

E

E

A

E

R

A

A

-

R

V

V

S

E

R

E

A

A

M

L

K

A

A

A

T

L

G

S

I

I

T

S

D

D

L

L

A

R

R

D

Q

R

S

P

V

T

D
x
H

T
x
M

L
|
L

S
|
S

G

G

G
|
G

Q
|
Q

R

K

Q

R

R

R

A

V

W

A

I

I

A

A

M

G

V

A

L

V

A

A

Q

M

E

R

T

P

A

E

I

V

M

L

L

L

D

D

E
x
Q

P

P

T

T

T

A

W

G

L

L

D

D

I

L

S

A

H

G

Q

T

I

E

D

Q

L

L

E

T

L

L

S

R

E

G

L

L

N

-

Q

R

Q

A

K

A

G

G

Y

M

T

T

L

L

A

V

A

F

V

S

L

T

H
|
H

D

D

L

V

N

E

Q

L

A

A

C

A

R

A

Y

L

A

A

T

D

H

R

L

V

I

A

A

L

L

F

R

R

E

T

G

G

K

R

I

V

V

L

A

A

E

E

G

G

A

A

P

A

K

E

E

A

I

V

V

L

S

S

A

D

E

R

L

A

I

T

E

L

K

A

I

K

Y

V

G

A

L

L

R

R

C

P

T

P

I

L

I

V

E

I

D

D

binding phosphomethylphosphonic acid adenylate ester: F14 (≠ Y17), V18 (≠ K20), A20 (≠ I22), N40 (= N42), G41 (= G43), G43 (= G45), K44 (= K46), S45 (= S47), T46 (= T48), Q88 (= Q88), H139 (≠ D142), M140 (≠ T143), L141 (= L144), S142 (= S145), G144 (= G147), Q145 (= Q148), Q166 (≠ E169), H198 (= H202)
binding magnesium ion: S45 (= S47), Q88 (= Q88)

P0AAH0 Phosphate import ATP-binding protein PstB; ABC phosphate transporter; Phosphate-transporting ATPase; EC 7.3.2.1 from Escherichia coli (strain K12) (see paper)
33% identity, 83% coverage: 6:223/264 of query aligns to 9:231/257 of P0AAH0

query
sites
P0AAH0

S

S

R

K

L

I

R

Q

G

V

D

R

Q

N

L

L

T

N

L

F

A

Y

Y

Y

G

G

K

K

K

F

T

H

I

A

A

L

E

K

S

N

L

I

N

N

V

L

T

D

I

I

P

A

D

K

G

N

H

Q

F

V

T

T

A

A

I

F

I

I

G

G

P

P

N

S

G

G

C

C

G
|
G

K
|
K

S

S

T

T

L

L

R

R

T

T

L

F

S

N

R

K

L

M

-

F

-

E

M

L

T

Y

P

P

-

E

-

Q

-

R

A

A

S

E

G

G

H

E

V

I

Y

L

L

L

D

D

G

G

E

D

Q

N

I

I

Q

L

R

T

Y

-

A

N

S

S

K

Q

E

D

V

I

A

A

-

L

-

R

K

A

R

K

I

V

G

G

L

M

L

V

A

F

Q

Q

N

K

A

P

T

-

T

T

P

P

G

F

D

P

I

M

T

S

V

I

Q

Y

E

D

L

N

V

I

A

A

R

F

G

G

R

V

Y

R

P

-

H

-

Q

-

P

-

L

L

F

F

T

E

R

K

W

L

R

S

K

R

E

A

D

D

E

M

E

D

-

E

-

R

-

V

-

Q

-

W

A

A

V

L

S

T

R

K

A

A

M

A

K

L

A

W

T

N

G

E

I

T

T

K

D

D

L

K

A

L

R

H

Q

Q

S

S

V

G

D

Y

T

S

L

L

S

S

G

G

Q

Q

R

Q

Q

Q

R

R

A

L

W

C

I

I

A

A

M

R

V

G

L

I

A

A

Q

I

E

R

T

P

A

E

I

V

M

L

L

L

D

D

E

E

P

P

T

C

T

S

W

A

L

L

D

D

I

P

S

I

H

S

Q

T

I

G

D

R

L

I

L

E

E

E

L

L

L

I

S

T

E

E

L

L

N

K

Q

Q

Q

D

K

-

G

-

Y

Y

T

T

L

V

A

V

A

I

V

V

L

T

H

H

D

N

L

M

N

Q

Q

Q

A

A

C

A

R

R

Y

C

A

S

T

D

H

H

L

T

I

A

A

F

L

M

R

Y

E

L

G

G

K

E

I

L

V

I

G48 (= G45) mutation to I: Loss of phosphate transport.
K49 (= K46) mutation to Q: Loss of phosphate transport.

Sites not aligning to the query:

1 modified: Initiator methionine, Removed

7mdyC Lolcde nucleotide-bound
35% identity, 77% coverage: 22:225/264 of query aligns to 21:223/226 of 7mdyC

query
sites
7mdyC

I

V

A

L

E

H

S

N

L

V

N

S

V

F

T

S

I

V

P

G

D

E

G

G

H

E

F

M

T

M

A

A

I

I

I

V

G

G

P

S

N

S

G
|
G

C
x
S

G
|
G

K
|
K

S
|
S

T
|
T

L

L

R

H

T

L

L

L

S

G

R

G

L

L

M

D

T

T

P

P

A

T

S

S

G

G

H

D

V

V

Y

I

L

F

D

N

G

G

E

Q

Q

P

I

M

Q

S

R

K

Y

L

A

S

S

S

K

A

E

A

V

K

A

A

-

E

-

L

-

R

-

N

K

Q

R

K

I

L

G

G

L

F

L

I

A

Y

Q
|
Q

N

F

A

H

T

H

T

L

P

L

G

P

D

D

I

F

T

T

V

A

Q

L

E

E

L

N

V

V

A

A

R

-

G

-

R

-

Y

-

P

-

H

-

Q

M

P

P

L

L

F

L

T

I

R

-

W

G

R

K

K

K

E

K

D

P

E

A

E

E

A

I

V

N

S

S

R

R

A

A

M

L

-

E

-

M

-

L

K

K

A

A

T

V

G

G

I

L

T

D

D

H

L

R

A

A

R

N

Q
x
H

S

R

V

P

D

S

T
x
E

L

L

S
|
S

G
|
G

Q

E

R

R

Q

Q

R

R

A

V

W

A

I

I

A

A

M

R

V

A

L

L

A

V

Q

N

E

N

T

P

A

R

I

L

M

V

L

L

L

A

D

D

E
|
E

P

P

T

T

T

G

W
x
N

L

L

D

D

I

A

S

R

H

N

Q

A

I

D

D

S

L

I

L

F

E

Q

L

L

S

G

E

E

L

L

N

N

Q

R

Q

L

K

Q

G

G

Y

T

T

A

L

F

A

L

A

V

V

V

L

T

H
|
H

D

D

L

L

N

Q

Q

L

A

A

C

K

R

R

Y

M

A

S

T

R

H

Q

L

L

I

-

A

E

L

M

R

R

E

D

G

G

K

R

I

L

V

T

A

A

E

E

binding adp orthovanadate: G42 (= G43), S43 (≠ C44), G44 (= G45), K45 (= K46), S46 (= S47), T47 (= T48), Q91 (= Q88), H138 (≠ Q139), E142 (≠ T143), S144 (= S145), G145 (= G146), G146 (= G147), E168 (= E169), N172 (≠ W173), H201 (= H202)
binding magnesium ion: S46 (= S47), Q91 (= Q88)

Sites not aligning to the query:

binding adp orthovanadate: 12

P75957 Lipoprotein-releasing system ATP-binding protein LolD; EC 7.6.2.- from Escherichia coli (strain K12) (see paper)
35% identity, 77% coverage: 22:225/264 of query aligns to 24:226/233 of P75957

query
sites
P75957

I

V

A

L

E

H

S

N

L

V

N

S

V

F

T

S

I

V

P

G

D

E

G

G

H

E

F

M

T

M

A

A

I

I

I

V

G
|
G

P

S

N

S

G

G

C

S

G

G

K

K

S

S

T

T

L

L

R

H

T

L

L

L

S

G

R

G

L

L

M

D

T

T

P

P

A

T

S

S

G

G

H

D

V

V

Y

I

L

F

D

N

G

G

E

Q

Q

P

I

M

Q

S

R

K

Y

L

A

S

S

S

K

A

E

A

V

K

A

A

-

E

-

L

-

R

-

N

K

Q

R

K

I

L

G

G

L

F

L

I

A

Y

Q

Q

N

F

A

H

T

H

T

L

P

L

G

P

D

D

I

F

T

T

V

A

Q

L

E

E

L

N

V

V

A

A

R

-

G

-

R

-

Y

-

P

-

H

-

Q

M

P

P

L

L

F

L

T

I

R

-

W

G

R

K

K

K

E

K

D

P

E

A

E

E

A

I

V

N

S

S

R

R

A

A

M

L

-

E

-

M

-

L

K

K

A

A

T

V

G

G

I

L

T

D

D

H

L

R

A

A

R

N

Q

H

S

R

V

P

D

S

T

E

L

L

S

S

G

G

Q

E

R

R

Q

Q

R

R

A

V

W

A

I

I

A

A

M

R

V

A

L

L

A

V

Q

N

E

N

T

P

A

R

I

L

M

V

L

L

L

A

D

D

E

E

P

P

T

T

T

G

W

N

L

L

D

D

I

A

S

R

H

N

Q

A

I

D

D

S

L

I

L

F

E

Q

L

L

S

G

E

E

L

L

N

N

Q

R

Q

L

K

Q

G

G

Y

T

T

A

L

F

A

L

A

V

V

V

L

T

H

H

D

D

L

L

N

Q

Q

L

A

A

C

K

R

R

Y

M

A

S

T

R

H

Q

L

L

I

-

A

E

L

M

R

R

E

D

G

G

K

R

I

L

V

T

A

A

E

E

G42 (= G40) mutation to D: Loss of lipoprotein release when overexpressed.

P69874 Spermidine/putrescine import ATP-binding protein PotA; EC 7.6.2.11 from Escherichia coli (strain K12) (see 3 papers)
30% identity, 89% coverage: 16:249/264 of query aligns to 26:260/378 of P69874

query
sites
P69874

A
x
C

Y
x
F

G

D

K

G

K

K

T

E

I

V

A

I

E

P

S

Q

L

L

N

D

V

L

T

T

I

I

P

N

D

N

G

G

H

E

F
|
F

T

L

A

T

I

L

G

G

P

P

N

S

G

G

C
|
C

G

G

K

K

S

T

T

T

L

V

L
|
L

R

R

T

L

L

I

S

A

R

G

L

L

M

E

T

T

P

V

A

D

S

S

G

G

H

R

V

I

Y

M

L
|
L

D

D

G

N

E

E

Q

D

I

I

Q

T

R

H

Y

V

A

P

S

A

K

E

E

N

V

-

A

-

K

R

R

Y

I

V

G

N

L

T

L

V

A

F

Q

Q

N

S

A

Y

T

A

T

L

P

F

G

P

D

H

I

M

T

T

V

V

Q

F

E

E

L

N

V

V

A

A

R

F

G

G

-

L

R

R

Y

M

P

Q

H

K

Q

T

P

P

L

A

F

-

T

-

R

-

W

-

R

-

K

A

E

E

D

I

E

T

E

P

A

R

V

V

S

M

R

E

A

A

M

L

K

R

A

M

T
x
V

G

Q

I

L

T

E

D

T

L

F

A

A

R

Q

Q

R

S

K

V

P

D

H

T

Q

L

L

S

S

G

G

Q

Q

R

Q

Q

Q

R

R

A

V

W

A

I

I

A

A

M

R

V

A

L

V

A

V

Q

N

E

K

T

P

A

R

I

L

M

L

L

L

D
|
D

E

E

P

S

T

L

T

S

W

A

L

L

D

D

I

Y

S

K

H

L

Q

R

I

K

D

Q

L

M

L

Q

E

N

L

E

L

L

S

K

E

A

L

L

N

Q

Q

R

Q

K

K

L

G

G

Y

I

T

T

L

F

A

V

A

F

V

V

L

T

H

H

D

D

L

Q

N

E

Q

E

A

A

C

L

R

T

Y

M

A

S

T

D

H

R

L

I

I

V

A

V

L

M

R

R

E

D

G

G

K

R

I

I

V

E

A

Q

E

D

G

G

A

T

P

P

K

R

E

E

I

I

V

Y

S

E

-

E

-

P

-

K

-

N

-

L

-

F

-

V

A

A

E

G

L

F

I

I

E

G

K

E

I

I

Y

N

G

M

L

F

R

N

C

A

T

T

I

V

I

I

E

E

C26 (≠ A16) mutation to A: Lower ATPase activity and transport efficiency.
F27 (≠ Y17) mutation to L: Lower ATPase activity and transport efficiency.
F45 (= F35) mutation to L: Lower ATPase activity and transport efficiency.
C54 (= C44) mutation to T: Loss of ATPase activity and transport.
L60 (= L50) mutation to F: Lower ATPase activity and transport efficiency.
L76 (= L66) mutation to P: Lower ATPase activity and transport efficiency.
V135 (≠ T131) mutation to M: Loss of ATPase activity and transport.
D172 (= D168) mutation to N: Loss of ATPase activity and transport.

Sites not aligning to the query:

276 C→A: Lower ATPase activity and transport efficiency.
297 mutation E->K,D: Lower ATPase activity and transport efficiency.; E→Q: Loss of ATPase activity and transport.

7v8iD Lolcd(e171q)e with bound amppnp in nanodiscs (see paper)
34% identity, 77% coverage: 22:225/264 of query aligns to 23:225/229 of 7v8iD

query
sites
7v8iD

I
x
V

A

L

E

H

S

N

L

V

N

S

V

F

T

S

I

V

P

G

D

E

G

G

H

E

F

M

T

M

A

A

I

I

I

V

G

G

P

S

N
x
S

G
|
G

C

S

G
|
G

K
|
K

S
|
S

T
|
T

L

L

R

H

T

L

L

L

S

G

R

G

L

L

M

D

T

T

P

P

A

T

S

S

G

G

H

D

V

V

Y

I

L

F

D

N

G

G

E

Q

Q

P

I

M

Q

S

R

K

Y

L

A

S

S

S

K

A

E

A

V

K

A

A

-

E

-

L

-

R

-

N

K

Q

R

K

I

L

G

G

L

F

L

I

A

Y

Q
|
Q

N

F

A

H

T

H

T

L

P

L

G

P

D

D

I

F

T

T

V

A

Q

L

E

E

L

N

V

V

A

A

R

-

G

-

R

-

Y

-

P

-

H

-

Q

M

P

P

L

L

F

L

T

I

R

-

W

G

R

K

K

K

E

K

D

P

E

A

E

E

A

I

V

N

S

S

R

R

A

A

M

L

-

E

-

M

-

L

K

K

A

A

T

V

G

G

I

L

T

D

D

H

L
x
R

A

A

R

N

Q
x
H

S

R

V

P

D

S

T
x
E

L

L

S
|
S

G

G

G
|
G

Q
x
E

R

R

Q

Q

R

R

A

V

W

A

I

I

A

A

M

R

V

A

L

L

A

V

Q

N

E

N

T

P

A

R

I

L

M

V

L

L

L

A

D

D

E

Q

P

P

T

T

T

G

W

N

L

L

D

D

I

A

S

R

H

N

Q

A

I

D

D

S

L

I

L

F

E

Q

L

L

S

G

E

E

L

L

N

N

Q

R

Q

L

K

Q

G

G

Y

T

T

A

L

F

A

L

A

V

V

V

L

T

H
|
H

D

D

L

L

N

Q

Q

L

A

A

C

K

R

R

Y

M

A

S

T

R

H

Q

L

L

I

-

A

E

L

M

R

R

E

D

G

G

K

R

I

L

V

T

A

A

E

E

binding phosphoaminophosphonic acid-adenylate ester: V23 (≠ I22), S43 (≠ N42), G44 (= G43), G46 (= G45), K47 (= K46), S48 (= S47), T49 (= T48), Q93 (= Q88), R137 (≠ L136), H140 (≠ Q139), E144 (≠ T143), S146 (= S145), G148 (= G147), E149 (≠ Q148), H203 (= H202)
binding magnesium ion: S48 (= S47), Q93 (= Q88)

7arlD Lolcde in complex with lipoprotein and adp (see paper)
34% identity, 77% coverage: 22:224/264 of query aligns to 21:222/222 of 7arlD

query
sites
7arlD

I

V

A

L

E

H

S

N

L

V

N

S

V

F

T

S

I

V

P

G

D

E

G

G

H

E

F

M

T

M

A

A

I

I

I

V

G

G

P

S

N

S

G
|
G

C

S

G
|
G

K
|
K

S
|
S

T

T

L

L

R

H

T

L

L

L

S

G

R

G

L

L

M

D

T

T

P

P

A

T

S

S

G

G

H

D

V

V

Y

I

L

F

D

N

G

G

E

Q

Q

P

I

M

Q

S

R

K

Y

L

A

S

S

S

K

A

E

A

V

K

A

A

-

E

-

L

-

R

-

N

K

Q

R

K

I

L

G

G

L

F

L

I

A

Y

Q

Q

N

F

A

H

T

H

T

L

P

L

G

P

D

D

I

F

T

T

V

A

Q

L

E

E

L

N

V

V

A

A

R

-

G

-

R

-

Y

-

P

-

H

-

Q

M

P

P

L

L

F

L

T

I

R

-

W

G

R

K

K

K

E

K

D

P

E

A

E

E

A

I

V

N

S

S

R

R

A

A

M

L

-

E

-

M

-

L

K

K

A

A

T

V

G

G

I

L

T

D

D

H

L

R

A

A

R

N

Q

H

S

R

V

P

D

S

T

E

L

L

S

S

G

G

Q

E

R

R

Q

Q

R

R

A

V

W

A

I

I

A

A

M

R

V

A

L

L

A

V

Q

N

E

N

T

P

A

R

I

L

M

V

L

L

L

A

D

D

E

E

P

P

T

T

T

G

W

N

L

L

D

D

I

A

S

R

H

N

Q

A

I

D

D

S

L

I

L

F

E

Q

L

L

S

G

E

E

L

L

N

N

Q

R

Q

L

K

Q

G

G

Y

T

T

A

L

F

A

L

A

V

V

V

L

T

H

H

D

D

L

L

N

Q

Q

L

A

A

C

K

R

R

Y

M

A

S

T

R

H

Q

L

L

I

-

A

E

L

M

R

R

E

D

G

G

K

R

I

L

V

T

A

A

binding adenosine-5'-diphosphate: G42 (= G43), G44 (= G45), K45 (= K46), S46 (= S47)

P19566 Maltose/maltodextrin import ATP-binding protein MalK; EC 7.5.2.1 from Salmonella typhimurium (strain LT2 / SGSC1412 / ATCC 700720) (see paper)
29% identity, 83% coverage: 12:231/264 of query aligns to 8:221/369 of P19566

query
sites
P19566

Q

N

L

V

T

T

L

K

A

A

Y

W

G

G

K

D

K

V

T

V

I

V

A

S

E

K

S

D

L

I

N

N

V

L

T

D

I

I

P

H

D

D

G

G

H

E

F

F

T

V

A

V

I

F

I

V

G

G

P

P

N

S

G

G

C

C

G

G

K

K

S

S

T

T

L

L

R

R

T

M

L

I

S

A

R

G

L

L

M

E

T

T

P

I

A

T

S

S

G

G

H

D

V

L

Y

F

L

I

D

G

G

E

E

T

Q

R

I

M

Q

N

R

D

Y

I

A

P

S

P

K

A

E

E

V

-

A

-

K

R

R

G

I

V

G

G

L

M

L

V

A

F

Q

Q

N

S

A

Y

T

A

T
x
L

P

Y

G

P

D

H

I

L

T

S

V

V

Q

A

E

E

L

N

V

M

A

S

R

F

G

G

R

L

Y

K

P

-

H

-

Q

-

P

-

L

L

F

A

T

G

R

A

W

K

R

K

K

E

E

V

D

M

E

N

E

Q

A

R

V

V

S

N

R

Q

A

V

M

A

K

E

A

V

T

L

G

Q

I

L

T

A

D

H

L

L

A

L

R

E

Q

R

S

K

V

P

D

K

T

A

L

L

S

S

G

G

Q

Q

R

R

Q

Q

R

R

A

V

W

A

I

I

A

G

M

R

V

T

L

L

A

V

Q

A

E

E

T

P

A

R

I

V

M

F

L

L

D

D

E

E

P
|
P

T

L

T

S

W

N

L

L

D
|
D

I

A

S

A

H

L

Q

R

I

V

D

Q

L

M

L

R

E

I

L

E

L

I

S

S

E

R

L

L

N

H

Q

K

Q

R

K

L

G

G

Y

R

T

T

L

M

A

I

A

Y

V

V

L

T

H

H

D

D

L

Q

N

V

Q

E

A

A

C

M

R

T

Y

L

A

A

T

D

H

K

L

I

I

V

A

V

L

L

R

D

E

A

G

G

K

R

I

V

V

A

A

Q

E

V

G

G

A

K

P

P

K

L

E

E

I

L

L86 (≠ T92) mutation to F: Loss of transport. No effect on ATP-binding activity but decrease in ATP hydrolysis. Retains repressor activity.
P160 (= P170) mutation to L: Loss of transport. No effect on ATP-binding activity but decrease in ATP hydrolysis. Retains repressor activity.
D165 (= D175) mutation to N: Loss of transport. No effect on ATP-binding activity but decrease in ATP hydrolysis. Retains repressor activity.

Sites not aligning to the query:

306 E→K: Loss of transport. No effect on ATP-binding and ATP hydrolysis. Retains repressor activity.

Query Sequence

>BWI76_RS07720 BWI76_RS07720 iron-enterobactin transporter ATP-binding protein
MTAVTSRLRGDQLTLAYGKKTIAESLNVTIPDGHFTAIIGPNGCGKSTLLRTLSRLMTPA
SGHVYLDGEQIQRYASKEVAKRIGLLAQNATTPGDITVQELVARGRYPHQPLFTRWRKED
EEAVSRAMKATGITDLARQSVDTLSGGQRQRAWIAMVLAQETAIMLLDEPTTWLDISHQI
DLLELLSELNQQKGYTLAAVLHDLNQACRYATHLIALREGKIVAEGAPKEIVSAELIEKI
YGLRCTIIEDPVAHTPLVVPLGRR

Or try a new SitesBLAST search

SitesBLAST's Database

SitesBLAST's database includes (1) SwissProt entries with experimentally-supported functional features; and (2) protein structures with bound ligands, from the BioLip database.

by Morgan Price, Arkin group
Lawrence Berkeley National Laboratory