SitesBLAST
Comparing BWI76_RS08460 FitnessBrowser__Koxy:BWI76_RS08460 to proteins with known functional sites using BLASTp with E ≤ 0.001.
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Found 20 (the maximum) hits to proteins with known functional sites (download)
O59010 Glutamate transporter homolog; Glt(Ph); Sodium-aspartate symporter Glt(Ph); Sodium-dependent aspartate transporter from Pyrococcus horikoshii (strain ATCC 700860 / DSM 12428 / JCM 9974 / NBRC 100139 / OT-3) (see 3 papers)
34% identity, 91% coverage: 8:405/435 of query aligns to 14:418/425 of O59010
- S65 (≠ T62) mutation to V: Strongly decreased chloride conductance.
- R276 (≠ S269) mutation to S: Increased rate of aspartate transport; when associated with R-395.
- RSS 276:278 (≠ SSS 269:271) binding
- M311 (≠ L304) mutation to A: Decreased dependence of aspartate binding on Na(+) concentration.
- T314 (≠ S307) binding
- V355 (= V348) binding
- D394 (= D381) binding
- M395 (= M382) mutation to R: Increased rate of aspartate transport; when associated with S-276.
- R397 (= R384) mutation to A: Strongly decreased affinity for aspartate.
- N401 (= N388) binding
- D405 (≠ N392) mutation to N: Strongly decreased affinity for aspartate.
6bavA Crystal structure of gltph r397c in complex with s-benzyl-l-cysteine (see paper)
34% identity, 91% coverage: 8:404/435 of query aligns to 6:409/409 of 6bavA
6bauA Crystal structure of gltph r397c in complex with l-cysteine (see paper)
33% identity, 91% coverage: 8:403/435 of query aligns to 6:408/408 of 6bauA
- binding cysteine: S270 (= S271), M303 (≠ L304), T306 (≠ S307), A345 (= A346), G346 (= G347), V347 (= V348), G351 (≠ S352), D386 (= D381), C389 (≠ R384), T390 (≠ S385), N393 (= N388)
2nwwA Crystal structure of gltph in complex with tboa (see paper)
32% identity, 91% coverage: 8:403/435 of query aligns to 5:407/407 of 2nwwA
6x15A Inward-facing state of the glutamate transporter homologue gltph in complex with l-aspartate and sodium ions (see paper)
32% identity, 91% coverage: 8:404/435 of query aligns to 14:417/419 of 6x15A
- binding [(2~{R})-1-[2-azanylethoxy(oxidanyl)phosphoryl]oxy-3-hexadecanoyloxy-propan-2-yl] (~{Z})-octadec-9-enoate: F46 (= F43), F46 (= F43), P75 (≠ D72), L91 (≠ C89), F95 (≠ L93), L130 (≠ F134), I133 (vs. gap), I159 (= I160), Y167 (vs. gap), K196 (= K189), G200 (≠ Y193), I207 (≠ L200), F210 (= F203), L250 (≠ G241), I262 (≠ T255), M269 (≠ A262), T334 (≠ S327), V335 (≠ M328), G336 (= G329), T340 (= T333), L343 (= L336), M399 (≠ A386)
- binding aspartic acid: S277 (= S270), S278 (= S271), T314 (≠ S307), G354 (= G347), A358 (= A351), G359 (≠ S352), D394 (= D381), R397 (= R384), T398 (≠ S385)
- binding sodium ion: Y89 (≠ F87), T92 (≠ A90), S93 (= S91), G306 (= G299), T308 (≠ S301), N310 (= N303), N310 (= N303), M311 (≠ L304), D312 (≠ V305), S349 (= S342), I350 (≠ K343), T352 (≠ M345), N401 (= N388), V402 (= V389), D405 (≠ N392)
Sites not aligning to the query:
6x14A Inward-facing state of the glutamate transporter homologue gltph in complex with tfb-tboa (see paper)
32% identity, 91% coverage: 8:403/435 of query aligns to 11:413/413 of 6x14A
- binding [(2~{R})-1-[2-azanylethoxy(oxidanyl)phosphoryl]oxy-3-hexadecanoyloxy-propan-2-yl] (~{Z})-octadec-9-enoate: G66 (= G66), V83 (≠ F84), I157 (≠ F161), Y164 (vs. gap), K193 (= K189), T305 (≠ S301), I306 (≠ F302), I347 (≠ K343)
- binding (2~{S},3~{S})-2-azanyl-3-[[3-[[4-(trifluoromethyl)phenyl]carbonylamino]phenyl]methoxy]butanedioic acid: I13 (= I10), M199 (= M195), S275 (= S271), T311 (≠ S307), G356 (≠ S352), L384 (≠ M374), D391 (= D381), R394 (= R384)
Sites not aligning to the query:
6bmiA Crystal structure of gltph r397c in complex with l-serine (see paper)
32% identity, 91% coverage: 8:403/435 of query aligns to 6:396/396 of 6bmiA
6zl4A The structure of glutamate transporter homologue glttk in complex with the photo switchable compound (cis) (see paper)
31% identity, 91% coverage: 10:405/435 of query aligns to 11:415/424 of 6zl4A
- binding decyl-beta-d-maltopyranoside: L191 (≠ K189), G195 (≠ Y193), R282 (≠ D280)
- binding (2~{S},3~{S})-2-azanyl-3-[[4-[2-(4-methoxyphenyl)hydrazinyl]phenyl]methoxy]butanedioic acid: R271 (≠ S269), S272 (= S270), S273 (= S271), M307 (≠ L304), T310 (≠ S307), G353 (≠ R350), A354 (= A351), R394 (= R384), T395 (≠ S385)
Sites not aligning to the query:
6zgbA Glutamate transporter homologue glttk in complex with a photo cage compound (see paper)
31% identity, 91% coverage: 10:405/435 of query aligns to 12:416/425 of 6zgbA
6xwnB Structure of glutamate transporter homologue glttk in the presence of tboa inhibitor (see paper)
31% identity, 91% coverage: 10:405/435 of query aligns to 14:418/426 of 6xwnB
5e9sA Crystal structure of substrate-bound glutamate transporter homologue glttk (see paper)
31% identity, 91% coverage: 10:405/435 of query aligns to 14:418/427 of 5e9sA
- binding aspartic acid: R274 (≠ S269), S275 (= S270), S276 (= S271), T313 (≠ S307), G353 (= G347), V354 (= V348), A357 (= A351), G358 (≠ S352), D394 (= D381), R397 (= R384), T398 (≠ S385)
- binding decyl-beta-d-maltopyranoside: L194 (≠ K189), G198 (≠ Y193), Y202 (≠ F197)
- binding sodium ion: Y87 (≠ F87), T90 (≠ A90), S91 (= S91), S276 (= S271), G305 (= G299), A306 (≠ Y300), T307 (≠ S301), N309 (= N303), N309 (= N303), M310 (≠ L304), D311 (≠ V305), S348 (= S342), I349 (≠ K343), G350 (= G344), T351 (≠ M345), N401 (= N388), V402 (= V389), D405 (≠ N392)
6r7rA Crystal structure of the glutamate transporter homologue glttk in complex with d-aspartate (see paper)
30% identity, 91% coverage: 10:405/435 of query aligns to 7:407/416 of 6r7rA
- binding d-aspartic acid: R263 (≠ S269), S265 (= S271), M299 (≠ L304), T302 (≠ S307), T340 (≠ M345), G342 (= G347), V343 (= V348), G347 (≠ S352), D383 (= D381), R386 (= R384), T387 (≠ S385), N390 (= N388)
- binding decyl-beta-d-maltopyranoside: H23 (≠ N26), V212 (≠ M218), A216 (≠ G222)
7awmA Structure of the thermostabilized eaat1 cryst mutant in complex with l-asp, three sodium ions and the allosteric inhibitor ucph101 (see paper)
31% identity, 83% coverage: 46:406/435 of query aligns to 59:400/412 of 7awmA
- binding 2-Amino-5,6,7,8-tetrahydro-4-(4-methoxyphenyl)-7-(naphthalen-1-yl)-5-oxo-4H-chromene-3-carbonitrile: S88 (≠ L76), G89 (= G77), G92 (≠ F80), A95 (≠ T83), V96 (≠ F84), Y99 (≠ F87), M163 (≠ I160), F167 (≠ C164), F293 (= F294), V297 (≠ I298)
- binding aspartic acid: S268 (= S270), S269 (= S271), T306 (≠ S307), G346 (= G347), I347 (≠ V348), A350 (= A351), G351 (≠ S352), D380 (= D381), R383 (= R384), T384 (≠ S385)
5mjuA Structure of the thermostabilized eaat1 cryst mutant in complex with the competititve inhibitor tfb-tboa and the allosteric inhibitor ucph101 (see paper)
30% identity, 83% coverage: 46:406/435 of query aligns to 51:386/397 of 5mjuA
- binding 2-Amino-5,6,7,8-tetrahydro-4-(4-methoxyphenyl)-7-(naphthalen-1-yl)-5-oxo-4H-chromene-3-carbonitrile: L72 (≠ I67), S80 (≠ L76), G81 (= G77), G84 (≠ F80), Y91 (≠ F87), M156 (≠ I160), F160 (≠ C164), F286 (= F294), V290 (≠ I298)
- binding (2~{S},3~{S})-2-azanyl-3-[[3-[[4-(trifluoromethyl)phenyl]carbonylamino]phenyl]methoxy]butanedioic acid: I64 (≠ V59), I148 (= I152), S262 (= S271), S263 (≠ E272), A292 (≠ Y300), T293 (≠ S301), M296 (≠ L304), T299 (≠ S307), G329 (= G344), A336 (= A351), G337 (≠ S352), D366 (= D381), R369 (= R384), N373 (= N388)
P56564 Excitatory amino acid transporter 1; Glial high affinity glutamate transporter; High-affinity neuronal glutamate transporter; GluT-1; Sodium-dependent glutamate/aspartate transporter 1; GLAST-1; Solute carrier family 1 member 3 from Mus musculus (Mouse) (see paper)
28% identity, 87% coverage: 46:422/435 of query aligns to 87:524/543 of P56564
- N206 (vs. gap) modified: carbohydrate, N-linked (GlcNAc...) asparagine
- N216 (≠ S142) modified: carbohydrate, N-linked (GlcNAc...) asparagine
P43003 Excitatory amino acid transporter 1; Sodium-dependent glutamate/aspartate transporter 1; GLAST-1; Solute carrier family 1 member 3 from Homo sapiens (Human) (see 3 papers)
28% identity, 81% coverage: 46:399/435 of query aligns to 87:494/542 of P43003
- S363 (= S269) mutation to R: Loss of electrogenic glutamate transport. Strongly decreased L-aspartate and L-glutamate uptake combined with strongly increased permeability ot other ions; when associated with M-477.
- SSS 363:365 (= SSS 269:271) binding
- T396 (≠ S301) binding
- T402 (≠ S307) binding
- IPQAG 443:447 (≠ VPRAS 348:352) binding
- D476 (= D381) binding
- R477 (≠ M382) mutation to M: Strongly decreased L-aspartate and L-glutamate uptake combined with strongly increased permeability ot other ions; when associated with R-363.
- N483 (= N388) binding
Sites not aligning to the query:
- 523 Y→F: No effect on activity.
Q10901 Excitatory amino acid transporter; Sodium-dependent glutamate/ aspartate transporter from Caenorhabditis elegans (see paper)
26% identity, 93% coverage: 1:403/435 of query aligns to 16:460/503 of Q10901
- N177 (≠ E151) modified: carbohydrate, N-linked (GlcNAc...) asparagine
- N187 (vs. gap) modified: carbohydrate, N-linked (GlcNAc...) asparagine
7xr4A Structure of human excitatory amino acid transporter 2 (eaat2) in complex with glutamate (see paper)
27% identity, 91% coverage: 6:399/435 of query aligns to 10:411/425 of 7xr4A
8cv2A Human excitatory amino acid transporter 3 (eaat3) in an outward facing sodium-bound state (see paper)
28% identity, 89% coverage: 14:401/435 of query aligns to 15:421/433 of 8cv2A
- binding sodium ion: Y85 (≠ F87), T88 (≠ A90), T89 (≠ S91), G319 (= G299), A320 (≠ Y300), N323 (= N303), N323 (= N303), M324 (≠ L304), D325 (≠ V305), N408 (= N388), D412 (≠ N392)
7xr6A Structure of human excitatory amino acid transporter 2 (eaat2) in complex with way-213613 (see paper)
27% identity, 91% coverage: 6:399/435 of query aligns to 11:410/424 of 7xr6A
- binding (2S)-2-azanyl-4-[[4-[2-bromanyl-4,5-bis(fluoranyl)phenoxy]phenyl]amino]-4-oxidanylidene-butanoic acid: S280 (= S270), S281 (= S271), T318 (≠ S307), G363 (≠ S352), M367 (≠ I356), V385 (≠ M374), D388 (= D377), R395 (= R384), T396 (≠ S385)
- binding dodecyl beta-D-glucopyranoside: V16 (≠ I11), V19 (≠ L14), I20 (= I15), W389 (≠ P378)
- binding cholesterol hemisuccinate: R80 (= R78), R84 (≠ K82), I95 (≠ L93), I252 (≠ V247)
Query Sequence
>BWI76_RS08460 FitnessBrowser__Koxy:BWI76_RS08460
MKKISLTKMIILGLILGMIAGVIINNMASADTAKSYAQDISIFTTIFLRMVKMIIAPLVI
STLVVGIAKMGDAKTLGRIFSKTFFLFICASLLSIALGLVIVNLFQPGAGINFVPHDAGA
VAAVQSEPFTLKVFISHAVPTSIVDAMARNEILQIVVFSIFLGCSLAAIGEKAEPIVKVL
DSLVHVMLKLTGYVMLFAPLTVFAAISGLIAERGLGVMVSAGIFMGEFYLTLGMLWAILI
GLSTVIVGPCISRLTKAIFEPALLAFTTSSSEAAFPGTLDKLEKFGVSSKIASFVLPIGY
SFNLVGSMAYCSFATVFIAQACNVHLSMGEQITMLLILMLTSKGMAGVPRASMVVIAATL
NQFNIPEAGLILLMGVDPFLDMGRSATNVMSNAMGAAIVGRWEGEHYGEGCRGTAPVKKP
EQDRPASETSEVAMS
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SitesBLAST's Database
SitesBLAST's database includes
(1) SwissProt
entries with experimentally-supported functional features;
and (2) protein structures with bound ligands, from the
BioLip database.
by Morgan Price,
Arkin group
Lawrence Berkeley National Laboratory