SitesBLAST

SitesBLAST – Find functional sites

SitesBLAST

Comparing BWI76_RS08480 FitnessBrowser__Koxy:BWI76_RS08480 to proteins with known functional sites using BLASTp with E ≤ 0.001.

Or try Sites on a Tree, PaperBLAST, Conserved Domains, or compare to all protein structures

Found 5 hits to proteins with known functional sites (download)

6h9fB Structure of glutamate mutase reconstituted with bishomo-coenzyme b12 (see paper)
59% identity, 100% coverage: 1:481/481 of query aligns to 1:482/483 of 6h9fB

query
sites
6h9fB

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active site: R100 (= R99), E171 (= E170)
binding (2~{R},3~{R},4~{S},5~{R})-2-(6-aminopurin-9-yl)-5-propyl-oxolane-3,4-diol: R66 (= R67), A67 (= A68), G68 (= G69), T94 (= T93), N123 (= N122), K326 (= K325), E330 (= E329), I334 (= I333), P335 (= P334)
binding cobalamin: A97 (= A96), R100 (= R99), N123 (= N122), Y181 (= Y180), T220 (= T219), M294 (= M293), G295 (= G294), G296 (= G295), F297 (= F296), E330 (= E329), G333 (= G332), I334 (= I333), P410 (= P409), F471 (≠ N470)

6h9eB Structure of glutamate mutase reconstituted with homo-coenzyme b12 (see paper)
59% identity, 100% coverage: 1:481/481 of query aligns to 1:482/483 of 6h9eB

query
sites
6h9eB

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active site: R100 (= R99), E171 (= E170)
binding cobalamin: A97 (= A96), R100 (= R99), N123 (= N122), Y181 (= Y180), T220 (= T219), M294 (= M293), G295 (= G294), G296 (= G295), F297 (= F296), E330 (= E329), G333 (= G332), I334 (= I333), F471 (≠ N470)
binding (2~{R},3~{R},4~{S},5~{R})-2-(6-aminopurin-9-yl)-5-ethyl-oxolane-3,4-diol: R66 (= R67), A67 (= A68), G68 (= G69), T94 (= T93), N123 (= N122), K326 (= K325), E330 (= E329), I334 (= I333), P335 (= P334)

1i9cB Glutamate mutase from clostridium cochlearium: complex with adenosylcobalamin and substrate (see paper)
59% identity, 100% coverage: 1:481/481 of query aligns to 1:482/483 of 1i9cB

query
sites
1i9cB

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I

I

T

T

V

V

G

G

Y

Y

G

G

Q

E

V

C

G

G

S

N

L

M

T

I

Q

Q

D

D

I

I

A

A

A

A

I

L

R

R

S

C

L

L

R

E

E

E

L

Q

S

T

H

N

E

E

Y

Y

F

L

G

K

N

A

Y

Y

G

G

F

Y

D

N

D

D

Y

V

E

F

L

V

S

T

T

T

V

V

F

F

H

H

Q

Q

W

W

M
|
M

G
|
G

G
|
G

F
|
F

P

P

E

Q

D

D

E

E

S

S

K

K

A

A

F

F

A

G

I

V

I

I

S

V

W

T

G

A

A

T

A

T

V

I

A

A

G

A

M

L

S

A

G

G

A

A

T

T

K

K

V

V

I

I

T

V

K

K

S

T

P

P

H

H

E
|
E

A

A

F

I

G
|
G

I
|
I

P

P

T

T

A

K

A

E

A

A

N

N

A

A

Q

A

G

G

L

I

K

K

A

A

S

T

R

K

Q

M

M

A

L

L

N

N

M

M

V

L

S

E

D

G

Q

Q

K

R

F

M

P

P

P

M

C

S

A

K

A

E

V

L

D

E

Q

T

E

E

V

M

E

A

L

V

I

I

K

K

S

A

E

E

V

T

R

K

A

C

V

I

L

L

K

D

K

K

V

M

F

F

E

E

L

L

G

G

N

K

G

G

D

D

I

L

A

A

R

I

G

G

T

T

V

V

L

K

A

A

F

F

E

E

A

T

G

G

V

V

L

M

D

D

V

I

P

P

F

F

A

G

P

P

A

S

S

K

C

Y

N

N

A

A

G

G

K

K

I

M

L

M

P

P

V

V

R

R

D

D

N

N

S

L

G

G

A

C

I

V

R

R

V

Y

L

L

E

E

A

F

G

G

A

N

V

V

P

P

L

F

P

T

K

E

D

E

I

I

L

K

A

N

L

Y

H

N

H

R

D

E

Y

R

V

L

A

Q

E

E

R

R

A

A

Q

K

F

F

E

E

G

G

R

R

K

D

P

V

S

S

F

F

Q

Q

M

M

V

V

V

I

D

D

D

D

I

I

N
x
F

A

A

V

V

S

G

H

K

S

G

K

R

L

L

I

I

G

G

R

R

P

P

active site: R100 (= R99), E171 (= E170)
binding (2S,3S)-3-methyl-aspartic acid: R66 (= R67), R100 (= R99), R149 (= R148), H150 (= H149), E171 (= E170), Y177 (= Y176), Y181 (= Y180), F216 (= F215)
binding cobalamin: A97 (= A96), R100 (= R99), N123 (= N122), T220 (= T219), M294 (= M293), G295 (= G294), G296 (= G295), F297 (= F296), E330 (= E329), G333 (= G332), I334 (= I333), F471 (≠ N470)
binding glutamic acid: R66 (= R67), R100 (= R99), R149 (= R148), H150 (= H149), E171 (= E170), Y177 (= Y176), Y181 (= Y180), F216 (= F215)

1ccwB Structure of the coenzyme b12 dependent enzyme glutamate mutase from clostridium cochlearium (see paper)
59% identity, 100% coverage: 1:481/481 of query aligns to 1:482/483 of 1ccwB

query
sites
1ccwB

M

M

E

E

L

L

R

K

N

N

K

K

K

K

L

W

T

T

H

D

D

E

E

E

F

F

M

H

T

K

E

Q

R

R

H

E

Q

E

V

V

L

L

Q

Q

T

Q

W

W

H

P

T

T

G

G

K

K

D

E

V

V

E

D

H

-

F

L

E

Q

D

E

G

A

V

V

K

D

Y

Y

Q

L

Q

K

T

K

I

I

P

P

E

A

K

E

K

K

R

N

F

F

S

A

H

E

A

K

L

L

L

V

K

L

A

A

D

K

Q

K

E

K

G

G

K

I

T

T

L

M

S

A

Q

Q

P

P

R

R

A

A

G

G

V

V

A

A

L

L

M

L

D

D

E

E

H

H

I

I

A

E

L

L

L

L

K

R

T

Y

L

L

Q

Q

E

D

E

E

-

G

-

G

C

A

D

D

L

F

L

L

P

P

S

S

T

T

I

I

D

D

A

A

Y

Y

T

T

R
|
R

L

Q

N

N

R

R

Y

Y

E

D

E

E

A

C

A

E

V

N

G

G

I

I

Q

K

K

E

S

S

I

E

E

K

A

A

G

G

T

R

S

S

K

L

L

L

N

N

G

G

L

F

P

P

V

G

V

V

N

N

H

F

G

G

V

V

A

K

E

G

C

C

R

R

R

K

M

V

T

L

E

E

A

A

L

V

E

N

K

L

P

P

V

L

Q

Q

V

A

R

R

H

H

G

G

T

T

P

P

D

D

A

S

R

R

L

L

L

L

A

A

E

E

I

I

S

I

M

H

A

A

S

G

G

G

F

W

T

T

S

S

Y

N

E
|
E

G

G

G

G

G

G

I

I

S

S

Y

Y

N

N

I

V

P

P

Y
|
Y

A

A

K

K

R

N

V

V

T

T

L

I

E

E

K

K

S

S

I

L

R

L

D

D

W

W

Q

Q

Y

Y

C

C

D

D

R

R

L

L

M

V

G

G

L

F

Y

Y

E

E

E

E

H

Q

G

G

I

V

R

H

I

I

N

N

R

R

E

E

P

P

F

F

G

G

P

P

L

L

T
|
T

G

G

T

T

L

L

I

V

P

P

P

P

F

S

M

M

S

S

H

N

A

A

V

V

A

G

I

I

I

T

E

E

G

A

L

L

L

L

A

A

L

A

E

E

Q

Q

G

G

V

V

K

K

S

N

I

I

T

T

V

V

G

G

Y

Y

G

G

Q

E

V

C

G

G

S

N

L

M

T

I

Q

Q

D

D

I

I

A

A

A

A

I

L

R

R

S

C

L

L

R

E

E

E

L

Q

S

T

H

N

E

E

Y

Y

F

L

G

K

N

A

Y

Y

G

G

F

Y

D

N

D

D

Y

V

E

F

L

V

S

T

T

T

V

V

F

F

H

H

Q

Q

W

W

M
|
M

G
|
G

G
|
G

F
|
F

P

P

E

Q

D

D

E

E

S

S

K

K

A

A

F

F

A

G

I

V

I

I

S

V

W

T

G

A

A

T

A

T

V

I

A

A

G

A

M

L

S

A

G

G

A

A

T

T

K

K

V

V

I

I

T

V

K

K

S

T

P

P

H

H

E
|
E

A
|
A

F

I

G
|
G

I
|
I

P

P

T

T

A

K

A

E

A

A

N

N

A

A

Q

A

G

G

L

I

K

K

A

A

S

T

R

K

Q

M

M

A

L

L

N

N

M

M

V

L

S

E

D

G

Q

Q

K

R

F

M

P

P

P

M

C

S

A

K

A

E

V

L

D

E

Q

T

E

E

V

M

E

A

L

V

I

I

K

K

S

A

E

E

V

T

R

K

A

C

V

I

L

L

K

D

K

K

V

M

F

F

E

E

L

L

G

G

N

K

G

G

D

D

I

L

A

A

R

I

G

G

T

T

V

V

L

K

A

A

F

F

E

E

A

T

G

G

V

V

L

M

D

D

V

I

P

P

F

F

A

G

P

P

A

S

S

K

C

Y

N

N

A

A

G

G

K

K

I

M

L

M

P

P

V

V

R

R

D

D

N

N

S

L

G

G

A

C

I

V

R

R

V

Y

L

L

E

E

A

F

G

G

A

N

V

V

P

P

L

F

P

T

K

E

D

E

I

I

L

K

A

N

L

Y

H

N

H

R

D

E

Y

R

V

L

A

Q

E

E

R

R

A

A

Q

K

F

F

E

E

G

G

R

R

K

D

P

V

S

S

F

F

Q

Q

M

M

V

V

V

I

D

D

D

D

I

I

N
x
F

A

A

V

V

S

G

H

K

S

G

K

R

L

L

I

I

G

G

R

R

P

P

active site: R100 (= R99), E171 (= E170)
binding cyanocobalamin: R100 (= R99), Y181 (= Y180), T220 (= T219), M294 (= M293), G295 (= G294), G296 (= G295), F297 (= F296), E330 (= E329), A331 (= A330), G333 (= G332), I334 (= I333), F471 (≠ N470)

1cb7B Glutamate mutase from clostridium cochlearium reconstituted with methyl-cobalamin (see paper)
59% identity, 100% coverage: 1:481/481 of query aligns to 1:482/483 of 1cb7B

query
sites
1cb7B

M

M

E

E

L

L

R

K

N

N

K

K

K

K

L

W

T

T

H

D

D

E

E

E

F

F

M

H

T

K

E

Q

R

R

H

E

Q

E

V

V

L

L

Q

Q

T

Q

W

W

H

P

T

T

G

G

K

K

D

E

V

V

E

D

H

-

F

L

E

Q

D

E

G

A

V

V

K

D

Y

Y

Q

L

Q

K

T

K

I

I

P

P

E

A

K

E

K

K

R

N

F

F

S

A

H

E

A

K

L

L

L

V

K

L

A

A

D

K

Q

K

E

K

G

G

K

I

T

T

L

M

S

A

Q

Q

P

P

R

R

A

A

G

G

V

V

A

A

L

L

M

L

D

D

E

E

H

H

I

I

A

E

L

L

L

L

K

R

T

Y

L

L

Q

Q

E

D

E

E

-

G

-

G

C

A

D

D

L

F

L

L

P

P

S

S

T

T

I

I

D

D

A

A

Y

Y

T

T

R
|
R

L

Q

N

N

R

R

Y

Y

E

D

E

E

A

C

A

E

V

N

G

G

I

I

Q

K

K

E

S

S

I

E

E

K

A

A

G

G

T

R

S

S

K

L

L

L

N

N

G

G

L

F

P

P

V

G

V

V

N

N

H

F

G

G

V

V

A

K

E

G

C

C

R

R

R

K

M

V

T

L

E

E

A

A

L

V

E

N

K

L

P

P

V

L

Q

Q

V

A

R

R

H

H

G

G

T

T

P

P

D

D

A

S

R

R

L

L

L

L

A

A

E

E

I

I

S

I

M

H

A

A

S

G

G

G

F

W

T

T

S

S

Y

N

E
|
E

G

G

G

G

G

G

I

I

S

S

Y

Y

N

N

I

V

P

P

Y
|
Y

A

A

K

K

R

N

V

V

T

T

L

I

E

E

K

K

S

S

I

L

R

L

D

D

W

W

Q

Q

Y

Y

C

C

D

D

R

R

L

L

M

V

G

G

L

F

Y

Y

E

E

E

E

H

Q

G

G

I

V

R

H

I

I

N

N

R

R

E

E

P

P

F

F

G

G

P

P

L

L

T
|
T

G

G

T

T

L

L

I

V

P

P

P

P

F

S

M

M

S

S

H

N

A

A

V

V

A

G

I

I

I

T

E

E

G

A

L

L

L

L

A

A

L

A

E

E

Q

Q

G

G

V

V

K

K

S

N

I

I

T

T

V

V

G

G

Y

Y

G

G

Q

E

V

C

G

G

S

N

L

M

T

I

Q

Q

D

D

I

I

A

A

A

A

I

L

R

R

S

C

L

L

R

E

E

E

L

Q

S

T

H

N

E

E

Y

Y

F

L

G

K

N

A

Y

Y

G

G

F

Y

D

N

D

D

Y

V

E

F

L

V

S

T

T

T

V

V

F

F

H

H

Q

Q

W

W

M
|
M

G
|
G

G
|
G

F
|
F

P

P

E

Q

D

D

E

E

S

S

K

K

A

A

F

F

A

G

I

V

I

I

S

V

W

T

G

A

A

T

A

T

V

I

A

A

G

A

M

L

S

A

G

G

A

A

T

T

K

K

V

V

I

I

T

V

K

K

S

T

P

P

H

H

E
|
E

A

A

F

I

G
|
G

I
|
I

P

P

T

T

A

K

A

E

A

A

N

N

A

A

Q

A

G

G

L

I

K

K

A

A

S

T

R

K

Q

M

M

A

L

L

N

N

M

M

V

L

S

E

D

G

Q

Q

K

R

F

M

P

P

P

M

C

S

A

K

A

E

V

L

D

E

Q

T

E

E

V

M

E

A

L

V

I

I

K

K

S

A

E

E

V

T

R

K

A

C

V

I

L

L

K

D

K

K

V

M

F

F

E

E

L

L

G

G

N

K

G

G

D

D

I

L

A

A

R

I

G

G

T

T

V

V

L

K

A

A

F

F

E

E

A

T

G

G

V

V

L

M

D

D

V

I

P

P

F

F

A

G

P

P

A

S

S

K

C

Y

N

N

A

A

G

G

K

K

I

M

L

M

P

P

V

V

R

R

D

D

N

N

S

L

G

G

A

C

I

V

R

R

V

Y

L

L

E

E

A

F

G

G

A

N

V

V

P

P

L

F

P

T

K

E

D

E

I

I

L

K

A

N

L

Y

H

N

H

R

D

E

Y

R

V

L

A

Q

E

E

R

R

A

A

Q

K

F

F

E

E

G

G

R

R

K

D

P

V

S

S

F

F

Q

Q

M

M

V

V

V

I

D

D

D

D

I

I

N
x
F

A

A

V

V

S

G

H

K

S

G

K

R

L

L

I

I

G

G

R

R

P

P

active site: R100 (= R99), E171 (= E170)
binding co-methylcobalamin: R100 (= R99), Y181 (= Y180), T220 (= T219), M294 (= M293), G295 (= G294), G296 (= G295), F297 (= F296), E330 (= E329), G333 (= G332), I334 (= I333), F471 (≠ N470)

Query Sequence

>BWI76_RS08480 FitnessBrowser__Koxy:BWI76_RS08480
MELRNKKLTHDEFMTERHQVLQTWHTGKDVEHFEDGVKYQQTIPEKKRFSHALLKADQEG
KTLSQPRAGVALMDEHIALLKTLQEECDLLPSTIDAYTRLNRYEEAAVGIQKSIEAGTSK
LNGLPVVNHGVAECRRMTEALEKPVQVRHGTPDARLLAEISMASGFTSYEGGGISYNIPY
AKRVTLEKSIRDWQYCDRLMGLYEEHGIRINREPFGPLTGTLIPPFMSHAVAIIEGLLAL
EQGVKSITVGYGQVGSLTQDIAAIRSLRELSHEYFGNYGFDDYELSTVFHQWMGGFPEDE
SKAFAIISWGAAVAGMSGATKVITKSPHEAFGIPTAAANAQGLKASRQMLNMVSDQKFPP
CAAVDQEVELIKSEVRAVLKKVFELGNGDIARGTVLAFEAGVLDVPFAPASCNAGKILPV
RDNSGAIRVLEAGAVPLPKDILALHHDYVAERAQFEGRKPSFQMVVDDINAVSHSKLIGR
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SitesBLAST's Database

SitesBLAST's database includes (1) SwissProt entries with experimentally-supported functional features; and (2) protein structures with bound ligands, from the BioLip database.

by Morgan Price, Arkin group
Lawrence Berkeley National Laboratory