SitesBLAST

SitesBLAST – Find functional sites

SitesBLAST

Comparing BWI76_RS09755 BWI76_RS09755 phosphoserine transaminase to proteins with known functional sites using BLASTp with E ≤ 0.001.

Or try Sites on a Tree, PaperBLAST, Conserved Domains, or compare to all protein structures

Found 20 (the maximum) hits to proteins with known functional sites (download)

7t7jB Crystal structure of phosphoserine aminotransferase from klebsiella pneumoniae subsp. Pneumoniae in complex with pyridoxal phosphate
92% identity, 99% coverage: 3:362/362 of query aligns to 1:360/360 of 7t7jB

query
sites
7t7jB

Q

Q

V

V

Y

Y

N

N

F

F

S

S

G

G

P

P

A

A

M

M

L

L

P

P

A

A

E

E

V

V

L

L

K

K

L

L

A

A

Q

Q

K

Q

E

E

L

L

C

C

D

D

W

W

N

H

G

G

L

L

G

G

T

T

S

S

V

V

M

M

E

E

I

I

S

S

H

H

R

R

G

G

K

K

E

E

F

F

I

I

K

Q

V

V

A

A

E

E

A

A

E

E

Q

Q

D

D

F

F

R

R

S

A

L

L

N

N

I

I

P

P

S

S

N

N

Y

Y

K

K

V

V

L

L

F

F

C

C

H

H

G

G

G
|
G

R
|
R

G

G

Q

Q

F

F

A

A

G

G

I

I

P

P

L

L

N

N

L

I

L

L

G

G

D

D

K

K

T

K

V

V

A

A

D

D

Y

Y

V

V

D

D

A

A

G

G

Y

Y

W
|
W

A

A

S

S

A

A

V

V

K

K

E

E

A

A

H

K

K

K

Y

Y

C

C

T

T

P

P

N

N

V

V

I

I

D

D

A

A

K

K

I

I

T

T

V

V

D

D

G

G

L

K

R

R

A

A

V

V

K

K

P

P

M

M

S

S

E

E

W

W

Q

Q

L

L

T

T

P

P

G

G

A

A

Y

Y

L

L

H

H

Y

Y

C

C

P

P

N

N

E

E

T
|
T

I

I

D

D

G

G

I

I

A

A

I

I

E

D

E

E

A

T

P

P

S

N

F

F

G

G

D

D

V

V

V

I

V

V

T

T

A

A

D
|
D

L

F

S
|
S

S

S

T

T

I

I

L

L

S

S

R

R

E

E

I

I

D

D

V

V

S

N

R

R

Y

F

G

G

V

V

I

I

Y

Y

A

A

G

G

A

A

Q
|
Q

K
|
K

N

N

I

I

G

G

P

P

A

A

G

G

L

L

T

T

L

L

I

V

I

I

V

V

R

R

E

E

D

D

L

L

G

G

K

K

A

A

S

S

V

V

A

A

C

C

P

P

S

S

I

I

L

L

D

D

Y

Y

S

T

I

V

L

L

S

S

A

E

N

N

D

D

S

S

M

M

F

F

N
|
N

T
|
T

P

P

T

T

F

F

A

A

W

W

Y

Y

L

L

S

A

G

G

L

L

V

V

F

F

K

K

W

W

L

L

K

K

E

Q

Q

Q

G

G

V

V

A

A

M

M

D

D

K

K

I

I

N

N

Q

Q

K

K

A

A

D

E

L

L

Y

Y

S

G

T

V

I

I

D

D

N

N

S

S

A

G

F

F

Y

Y

R

R

N

N

D

D

V

V

A

A

A

Q

A

A

N

N

R

R

S

S

R

R

M

M

N

N

V

V

P

P

F

F

Q

Q

L

L

A

A

D

D

S

S

A

A

L

L

D

D

K

K

L

L

F

F

L

L

E

E

S

S

F

F

A

A

G

G

L

L

H

H

A

A

L

L

K

K

G

G

H

H

R

R

V

V

G

G

M

M

R

R

A

A

S

S

I

I

Y

Y

N

N

A

A

M

M

P

P

L

L

A

D

G

G

V

V

K

K

A

T

L

L

T

T

D

D

F

F

M

M

Q

L

D

D

F

F

E

E

R

R

H

H

G

G

binding pyridoxal-5'-phosphate: G73 (= G75), G74 (= G76), R75 (= R77), W100 (= W102), T151 (= T153), D172 (= D174), S174 (= S176), Q195 (= Q197), K196 (= K198), N237 (= N239), T238 (= T240)

1bjoA The structure of phosphoserine aminotransferase from e. Coli in complex with alpha-methyl-l-glutamate (see paper)
86% identity, 99% coverage: 3:362/362 of query aligns to 1:360/360 of 1bjoA

query
sites
1bjoA

Q

Q

V

I

Y

F

N

N

F

F

S

S

S
|
S

G

G

P

P

A

A

M

M

L

L

P

P

A

A

E

E

V

V

L

L

K

K

L

Q

A

A

Q

Q

K

Q

E

E

L

L

C

R

D

D

W

W

N

N

G

G

L

L

G

G

T

T

S

S

V

V

M

M

E

E

I

V

S

S

H

H

R

R

G

G

K

K

E

E

F

F

I

I

K

Q

V

V

A

A

E

E

A

A

E

E

Q

K

D

D

F

F

R

R

S

D

L

L

N

N

I

V

P

P

S

S

N

N

Y

Y

K

K

V

V

L

L

F

F

C

C

H

H

G

G

G
|
G

R
|
R

G

G

Q

Q

F

F

A

A

G

A

I

V

P

P

L

L

N

N

L

I

L

L

G

G

D

D

K

K

T

T

V

T

A

A

D

D

Y

Y

V

V

D

D

A

A

G

G

Y

Y

W
|
W

A

A

S

S

A

A

V

I

K

K

E

E

A

A

H

K

K

K

Y

Y

C

C

T

T

P

P

N

N

V

V

I

F

D

D

A

A

K

K

I

V

T

T

V

V

D

D

G

G

L

L

R

R

A

A

V

V

K

K

P

P

M

M

S

R

E

E

W

W

Q

Q

L

L

T

S

P

D

G

N

A

A

Y

Y

L

M

H

H

Y

Y

C

C

P

P

N

N

E

E

T
|
T

I

I

D

D

G

G

I

I

A

A

I

I

E

D

E

E

A

T

P

P

S

D

F

F

G

G

D

A

D

D

V

V

T

A

A

A

D
|
D

L

F

S
|
S

S

S

T

T

I

I

L

L

S

S

R

R

E

P

I

I

D

D

V

V

S

S

R

R

Y

Y

G

G

V

V

I

I

Y

Y

A

A

G

G

A

A

Q
|
Q

K
|
K

N

N

I

I

G

G

P

P

A

A

G

G

L

L

T

T

L

I

I

V

I

I

V

V

R

R

E

E

D

D

L

L

G

G

K

K

A

A

S

N

V

I

A

A

C

C

P

P

S

S

I

I

L

L

D

D

Y

Y

S

S

I

I

L

L

S

N

A

D

N

N

D

G

S

S

M

M

F

F

N

N

T

T

P

P

T

T

F

F

A

A

W

W

Y

Y

L

L

S

S

G

G

L

L

V

V

F

F

K

K

W

W

L

L

K

K

E

A

Q

N

G

G

V

V

A

A

A

E

M

M

D

D

K

K

I

I

N

N

Q

Q

K

K

A

A

D

E

L

L

Y

Y

S

G

T

V

I

I

D

D

N

N

S

S

A

D

F

F

Y

Y

R

R

N

N

D

D

V

V

A

A

A

K

A

R

N

N

R

R

S

S

R

R

M

M

N

N

V

V

P

P

F

F

Q

Q

L

L

A

A

D

D

S

S

A

A

L

L

D

D

K

K

L

L

F

F

L

L

E

E

S

S

F

F

A

A

G

G

L

L

H

H

A

A

L

L

K

K

G

G

H

H

R

R

V

V

G

G

M

M

R

R

A

A

S

S

I

I

Y

Y

N

N

A

A

M

M

P

P

L

L

A

E

G

G

V

V

K

K

A

A

L

L

T

T

D

D

F

F

M

M

Q

V

D

E

F

F

E

E

R

R

H

H

G

G

active site: W100 (= W102), D172 (= D174), K196 (= K198)
binding alpha-methyl-l-glutamic acid: S7 (= S9), W100 (= W102), T151 (= T153), K196 (= K198)
binding pyridoxal-5'-phosphate: G74 (= G76), R75 (= R77), W100 (= W102), T151 (= T153), D172 (= D174), S174 (= S176), Q195 (= Q197), K196 (= K198)

3qboB Crystal structure of phosphoserine aminotransferase from yersinia pestis co92
76% identity, 99% coverage: 3:362/362 of query aligns to 1:359/359 of 3qboB

query
sites
3qboB

Q

Q

V

V

Y

Y

N

N

F

F

S

S

S

A

G

G

P

P

A

A

M

M

L

L

P

P

A

V

E

E

V

V

L

L

K

R

L

R

A

A

Q

Q

K

Q

E

E

L

L

C

R

D

D

W

W

N

H

G

G

L

L

G

G

T

T

S

S

V

V

M

M

E

E

I

V

S

S

H

H

R

R

G

S

K

K

E

E

F

F

I

M

K

Q

V

V

A

A

E

E

A

S

E

E

Q

K

D

D

F

L

R

R

S

D

L

L

N

N

I

V

P

P

S

A

N

N

Y

Y

K

K

V

V

L

L

F

F

C

C

H

H

G

G

G
|
G

G
x
A

R
|
R

G

A

Q

Q

F

F

A

A

G

A

I

V

P

P

L

L

N

N

L

L

G

G

D

D

K

R

T

N

V

S

A

A

D

D

Y

Y

V

V

D

D

A

G

G

G

Y

Y

W
|
W

A

A

A

H

S

S

A

A

V

I

K

K

E

E

A

A

H

Q

K

K

Y

Y

C

C

T

T

P

P

N

N

V

V

I

I

D

D

A

V

K

T

I

T

T

H

V

D

D

N

G

G

L

L

R

T

A

G

V

I

K

Q

P

P

M

M

S

K

E

Q

W

W

Q

K

L

L

T

S

P

D

G

N

A

A

Y

Y

L

V

H

H

Y

Y

C

C

P

P

N

N

E

E

T
|
T

I

I

D

D

G

G

I

I

A

A

I

I

E

D

E

E

A

Q

P

P

S

D

F

F

G

G

D

N

D

K

V

V

T

-

A

A

D
|
D

L

F

S
|
S

S

S

T

S

I

I

L

L

S

S

R

R

E

P

I

I

D

D

V

V

S

S

R

R

Y

Y

G

G

V

V

I

I

Y

Y

A

A

G

G

A

A

Q

Q

K
|
K

N

N

I

I

G

G

P

P

A

A

G

G

L

L

T

T

L

L

I

V

I

I

V

V

R

R

E

E

D

D

L

L

G

G

K

K

A

A

S

H

V

T

A

A

C

L

P

P

S

S

I

I

L

L

D

D

Y

Y

S

K

I

I

L

L

S

A

A

D

N

N

D

D

S

S

M

M

F

F

N
|
N

T
|
T

P

P

T

T

F

F

A

A

W

W

Y

Y

L

L

S

S

G

G

L

L

V

V

F

F

K

K

W

W

L

L

K

K

E

E

Q

Q

G

G

V

L

A

G

A

E

M

M

D

E

K

K

I

R

N

N

Q

Q

Q

A

K

K

A

A

D

E

L

L

Y

Y

S

G

T

A

I

I

D

D

N

R

S

T

A

G

F

F

Y

Y

R

R

N

N

D

D

V

V

A

A

A

I

A

T

N

N

R

R

S

S

R

W

M

M

N

N

V

V

P

P

F

F

Q

Q

L

M

A

A

D

D

S

A

A

S

L

L

D

D

K

K

L

L

F

F

L

L

E

S

E

E

S

A

F

E

A

A

A

Q

G

G

L

L

H

Q

A

A

L

L

K

K

G

G

H

H

R

R

V

V

V

A

G

G

M

M

R

R

A

A

S

S

I

I

Y

Y

N

N

A

A

M

M

P

P

L

I

A

E

G

G

V

V

K

K

A

A

L

L

T

T

D

D

F

F

M

M

Q

A

D

D

F

F

E

E

R

R

H

H

G

G

binding pyridoxal-5'-phosphate: G73 (= G75), A74 (≠ G76), R75 (= R77), W100 (= W102), T151 (= T153), D171 (= D174), S173 (= S176), K195 (= K198), N236 (= N239), T237 (= T240)

3qm2B 2.25 angstrom crystal structure of phosphoserine aminotransferase (serc) from salmonella enterica subsp. Enterica serovar typhimurium
79% identity, 99% coverage: 3:362/362 of query aligns to 1:331/331 of 3qm2B

query
sites
3qm2B

Q

Q

V

V

Y

F

N

N

F

F

S

S

G

G

P

P

A

A

M

M

L

L

P

P

A

A

E

E

V

V

L

L

K

K

L

L

A

A

Q

Q

K

Q

E

E

L

L

C

-

D

-

W

-

N

-

G

-

L

-

G

-

T

-

S

-

V

-

M

-

E

-

I

-

S

-

H

-

R

-

G

-

K

-

E

-

F

-

I

-

K

-

V

V

A

A

E

E

A

A

E

E

Q

Q

D

D

F

F

R

R

S

D

L

L

N

N

I

I

P

P

S

S

N

N

Y

Y

K

K

V

V

L

L

F

F

C

C

H

H

G

G

R

R

G

G

Q

Q

F

F

A

A

G

G

I

V

P

P

L

L

N

N

L

L

G

G

D

D

K

K

T

T

V

T

A

A

D

D

Y

Y

V

V

D

D

A

A

G

G

Y

Y

W
|
W

A

A

S

S

A

A

V

I

K

K

E

E

A

A

H

K

K

K

Y

Y

C

C

T

A

P

P

N

Q

V

I

I

I

D

D

A

A

K

K

I

I

T

T

V

V

D

D

G

G

L

K

R

R

A

A

V

V

K

K

P

P

M

M

S

R

E

E

W

W

Q

Q

L

L

T

S

P

D

G

N

A

A

Y

Y

L

L

H

H

Y

Y

C

C

P

P

N

N

E

E

T

T

I

I

D

D

G

G

I

I

A

A

I

I

E

D

E

E

A

T

P

P

S

D

F

F

G

G

D

P

D

E

V

V

T

T

A

A

D
|
D

L

F

S

S

T

T

I

I

L

L

S

S

R

A

E

P

I

L

D

D

V

V

S

S

R

R

Y

Y

G

G

V

V

I

I

Y

Y

A

A

G

G

A

A

Q

Q

K
|
K

N

N

I

I

G

G

P

P

A

A

G

G

L

L

T

T

L

L

I

V

I

I

V

V

R

R

E

E

D

D

L

L

G

G

K

K

A

A

S

H

V

E

A

S

C

C

P

P

S

S

I

I

L

L

D

D

Y

Y

S

T

I

V

L

L

S

N

A

D

N

N

D

-

S

-

M

-

F

-

N

-

T

-

P

-

P

P

T

T

F

F

A

A

W

W

Y

Y

L

L

S

S

G

G

L

L

V

V

F

F

K

K

W

W

L

L

K

K

E

A

Q

Q

G

G

V

V

A

A

M

M

D

H

K

K

I

I

N

N

Q

Q

K

K

A

A

D

E

L

L

Y

Y

S

G

T

V

I
|
I

D
|
D

N

N

S
|
S

A

D

F

F

Y
|
Y

R

R

N

N

D

D

V

V

A

A

A

Q

A

A

N

N

R

R

S

S

R

R

M

M

N

N

V

V

P

P

F

F

Q

Q

L

L

A

A

D

D

S

N

A

T

L

L

D

D

K

K

L

V

F

F

L

L

E

E

S

S

F

F

A

A

G

G

L

L

H

H

A

A

L

L

K

K

G

G

H

H

R

R

V

V

G

G

M

M

R

R

A

A

S

S

I

I

Y

Y

N

N

A

A

M

M

P

P

L

I

A

E

G

G

V

V

K

K

A

A

L

L

T

T

D

D

F

F

M

M

Q

I

D

D

F

F

E

E

R

R

H

H

G

G

active site: W78 (= W102), D150 (= D174), K174 (= K198)
binding calcium ion: I249 (= I280), D250 (= D281), S252 (= S283), Y255 (= Y286)

6xdkD Crystal structure of phosphoserine aminotransferase (serc) from stenotrophomonas maltophilia k279a
53% identity, 99% coverage: 3:362/362 of query aligns to 1:359/359 of 6xdkD

query
sites
6xdkD

Q

R

V

A

Y

F

N

N

F

F

S

S

S

A

G

G

P

P

A

A

M

T

L

L

P

P

A

E

E

S

V

V

L

L

K

R

L

Q

A

A

Q

Q

K

A

E

E

L

M

C

L

D

D

W

W

N

H

G

G

L

S

G

G

T

A

S

S

V

I

M

V

E

E

I

M

S

S

H

H

R

R

G

G

K

A

E

E

F

F

I

M

K

S

V

V

A

A

E

A

E

E

A

A

E

E

Q

A

D

D

F

L

R

R

S

R

L

L

N

D

I

I

P

P

S

D

N

D

Y

Y

K

A

V

V

L

L

F

F

C

L

H

S

G

G

G

A

R

T

G

T

Q

Q

F

Q

A

A

G

L

I

I

P

P

L

L

N

N

L

F

L

A

G

A

D

P

K

G

T

Q

V

R

A

A

D

D

Y

Y

V

V

D

V

A

S

G

G

Y

H

W

W

A

G

A

K

S

T

A

A

V

V

K

K

E

Q

A

A

H

G

K

V

Y

Y

C

V

T

D

P

V

N

N

V

-

I

I

D

A

A

A

K

S

I

S

T

E

V

A

D

N

G

G

L

Y

R

R

A

E

V

L

K

P

P

A

M

R

S

A

E

D

W

W

Q

Q

L

L

T

S

P

R

G

D

A

A

Y

Y

L

V

H

H

Y

I

C

T

P

A

N

N

E

E

T

T

I

I

D

H

G

G

I

V

A

E

I

F

E

R

E

D

A

V

P

P

S

D

F

T

G

G

D

N

D

-

V

V

V

P

V

L

T

I

A

A

D

D

L

F

S

S

T

S

I

I

L

A

S

S

R

E

E

P

I

L

D

D

V

V

S

R

R

R

Y

Y

G

G

V

V

I

I

Y

Y

A

A

G

G

A

A

Q

Q

K

K

N

N

I

L

G

G

P

P

A

V

G

G

L

V

T

A

L

V

I

M

I

I

V

I

R

R

E

R

D

D

L

L

G

E

K

R

A

S

S

G

V

Q

A

P

C

R

P

A

S

D

I

I

L

F

D

D

Y

Y

S

R

I

S

L

H

S

V

A

A

N

R

D

D

S

S

M

M

F

L

N
|
N

T
|
T

P

P

T

T

F

W

A

N

W

W

Y

Y

L

L

S

A

G

G

L

L

V

V

F

F

K

K

W

W

L

M

K

L

E

A

Q

E

G

G

V

V

A

T

A

E

M

F

D

A

K

K

I

R

N

N

Q

A

K

K

A

A

D

A

L

L

L

V

Y

Y

S

G

T

A

I

I

D

D

N

G

S

S

-

G

A

G

F

F

Y

Y

R

R

N

N

D

E

V

V

A

A

A

Y

A

A

N

A

R

R

S

S

R

R

M

M

N

N

V

I

P

P

F

F

Q

F

L

L

A

P

D

D

S

A

A

E

L

L

D

D

K

A

L

R

F

F

L

V

E

A

E

E

S

A

F

K

A

A

G

G

L

L

H

L

A

A

L

L

K

K

G

G

H

H

R

K

V

V

G

G

M

I

R

R

A

A

S

S

I

L

Y

Y

N

N

A

A

M

M

P

P

L

L

A

A

G

G

V

A

K

E

A

A

L

L

T

V

D

A

F

F

M

M

Q

A

D

D

F

F

E

Q

R

Q

R

R

H

H

G

G

binding pyridoxal-5'-phosphate: N235 (= N239), T236 (= T240)

6xdkB Crystal structure of phosphoserine aminotransferase (serc) from stenotrophomonas maltophilia k279a
53% identity, 99% coverage: 3:362/362 of query aligns to 1:355/355 of 6xdkB

query
sites
6xdkB

Q

R

V

A

Y

F

N

N

F

F

S

S

S

A

G

G

P

P

A

A

M

T

L

L

P

P

A

E

E

S

V

V

L

L

K

R

L

Q

A

A

Q

Q

K

A

E

E

L

M

C

L

D

D

W

W

N

H

G

G

L

S

G

G

T

A

S

S

V

I

M

V

E

E

I

M

S

S

H

H

R

R

G

G

K

A

E

E

F

F

I

M

K

S

V

V

A

A

E

A

E

E

A

A

E

E

Q

A

D

D

F

L

R

R

S

R

L

L

N

D

I

I

P

P

S

D

N

D

Y

Y

K

A

V

V

L

L

F

F

C

L

H

S

G

G

G
x
A

R
x
T

G

T

Q

Q

F

Q

A

A

G

L

I

I

P

P

L

L

N

N

L

F

L

A

G

A

D

P

K

G

T

Q

V

R

A

A

D

D

Y

Y

V

V

D

V

A

S

G

G

Y

H

W
|
W

A

G

A

K

S

T

A

A

V

V

K

K

E

Q

A

A

H
x
G

K

V

Y

Y

C
x
V

T
x
D

P

V

N

N

V

-

I

I

D

A

A

A

K

S

I

S

T

E

V

A

D

N

G

G

L

Y

R

R

A

E

V

L

K

P

P

A

M

R

S

A

E

D

W

W

Q

Q

L

L

T

S

P

R

G

D

A

A

Y

Y

L

V

H

H

Y

I

C

T

P

A

N

N

E

E

T
|
T

I

I

D

H

G

G

I

V

A

E

I

F

E

R

E

D

A

V

P

P

S

D

F

T

G

G

D

-

D

N

V

V

V

P

V

L

T

I

A

A

D
|
D

L

F

S
|
S

S

S

T

S

I

I

L

A

S

S

R

E

E

P

I

L

D

D

V

V

S

R

R

R

Y

Y

G

G

V

V

I

I

Y

Y

A

A

G

G

A

A

Q

Q

K
|
K

N

N

I

L

G

G

P

P

A

V

G

G

L

V

T

A

L

V

I

M

I

I

V

I

R

R

E

R

D

D

L

L

G

E

K

R

A

S

S

G

V

Q

A

P

C

R

P

A

S

D

I

I

L

F

D

D

Y

Y

S

R

I

S

L

H

S

V

A

A

N

R

D

-

S

-

M

-

F

-

N

N

T

T

P

P

T

T

F

W

A

N

W

W

Y

Y

L

L

S

A

G

G

L

L

V

V

F

F

K

K

W

W

L

M

K

L

E

A

Q

E

G

G

V

V

A

T

A

E

M

F

D

A

K

K

I

R

N

N

Q

A

K

K

A

A

D

A

L

L

L

V

Y

Y

S

G

T

A

I

I

D

D

N

G

S

S

-

G

A

G

F

F

Y

Y

R

R

N

N

D

E

V

V

A

A

A

Y

A

A

N

A

R

R

S

S

R

R

M

M

N

N

V

I

P

P

F

F

Q

F

L

L

A

P

D

D

S

A

A

E

L

L

D

D

K

A

L

R

F

F

L

V

E

A

E

E

S

A

F

K

A

A

G

G

L

L

H

L

A

A

L

L

K

K

G

G

H

H

R

K

V

V

G

G

M

I

R

R

A

A

S

S

I

L

Y

Y

N

N

A

A

M

M

P

P

L

L

A

A

G

G

V

A

K

E

A

A

L

L

T

V

D

A

F

F

M

M

Q

A

D

D

F

F

E

Q

R

Q

R

R

H

H

G

G

binding calcium ion: G109 (≠ H111), V112 (≠ C114), D113 (≠ T115)
binding pyridoxal-5'-phosphate: A74 (≠ G76), T75 (≠ R77), W100 (= W102), T150 (= T153), D170 (= D174), S172 (= S176), K194 (= K198)

6czyA Crystal structure of arabidopsis thaliana phosphoserine aminotransferase isoform 1 (atpsat1) in complex with pyridoxamine-5'- phosphate (pmp) (see paper)
51% identity, 99% coverage: 2:361/362 of query aligns to 3:361/362 of 6czyA

query
sites
6czyA

A

A

Q

R

V

V

Y

F

N

N

F

F

S

A

G

G

P

P

A

A

M

T

L

L

P

P

A

E

E

N

V

V

L

L

K

L

L

K

A

A

Q

Q

K

A

E

D

L

L

C

Y

D

N

W

W

N

R

G

G

L

S

G

G

T

M

S

S

V

V

M

M

E

E

I

M

S

S

H

H

R

R

G

G

K

K

E

E

F

F

I

L

K

S

V

I

A

I

E

Q

E

K

A

A

E

E

Q

S

D

D

F

L

R

R

S

Q

L

L

N

E

I

I

P

P

S

Q

N

E

Y

Y

K

S

V

V

L

L

F

F

C

L

H

Q

G

G

G
|
G

G
x
A

R
x
T

G

T

Q

Q

F

F

A

A

G

A

I

L

P

P

L

L

N

N

L

L

L

C

G

K

D

S

K

D

T

D

V

T

A

V

D

D

Y

F

V

V

D

V

A

T

G

G

Y

S

W
|
W

A

G

A

D

S

K

A

A

V

V

K

K

E

E

A

A

H

K

K

K

Y

Y

C

C

T

K

P

T

N

N

V

V

I

I

D

W

A

S

K

G

I

K

T

S

V

-

D

E

G

K

L

Y

R

T

A

K

V

V

K

P

P

S

M

F

S

E

E

E

W

L

Q

E

L

Q

T

T

P

P

G

D

A

A

A

K

Y

Y

L

L

H

H

Y

I

C

C

P

A

N

N

E

E

T
|
T

I

I

D

H

G

G

I

V

A

E

I

F

E

K

E

D

A

Y

P

P

S

V

F

P

G

K

D

N

D

G

V

F

V

L

V

V

T

-

A

A

D
|
D

L

M

S

S

T

N

I

F

L

C

S

S

R

K

E

P

I

V

D

D

V

V

S

S

R

K

Y

F

G

G

V

V

I

I

Y

Y

A

G

G

G

A

A

Q
|
Q

K
|
K

N

N

I

V

G

G

P

P

A

S

G

G

L

V

T

T

L

I

I

V

I

I

V

I

R

R

E

K

D

D

L

L

L

I

G

G

K

N

A

A

S

Q

V

D

A

I

C

T

P

P

S

V

I

M

L

L

D

D

Y

Y

S

K

I

I

L

H

S

D

A

E

N

N

D

S

S

S

M

L

F

Y

N
|
N

T
|
T

P

P

T

C

F

F

A

G

W

I

Y

Y

L

M

S

C

G

G

L

L

V

V

F

F

K

E

W

D

L

L

K

L

E

E

Q

Q

G

G

V

L

A

K

A

E

M

V

D

E

K

K

I

K

N

N

Q

Q

Q

R

K

K

A

A

D

D

L

L

Y

Y

S

N

T

A

I

I

D

E

N

E

S

S

-

N

A

G

F

F

Y

F

R

R

N

C

D

P

V

V

A

E

A

K

A

S

N

V

R

R

S

S

R

L

M

M

N

N

V

V

P

P

F

F

Q

T

L

L

A

E

D

K

S

S

A

E

L

L

D

E

K

A

L

E

F

F

L

I

E

K

E

E

S

A

F

A

A

K

A

E

G

K

L

M

H

V

A

Q

L

L

K

K

G

G

H

H

R

R

V

S

V

V

G

G

M

M

R

R

A

A

S

S

I

I

Y

Y

N

N

A

A

M

M

P

P

L

L

A

A

G

G

V

V

K

E

A

K

L

L

T

V

D

A

F

F

M

M

Q

K

D

D

F

F

E

Q

R

A

R

K

H

H

binding 4'-deoxy-4'-aminopyridoxal-5'-phosphate: G76 (= G75), A77 (≠ G76), T78 (≠ R77), W103 (= W102), T153 (= T153), D173 (= D174), Q196 (= Q197), K197 (= K198), N238 (= N239), T239 (= T240)

6czzA Crystal structure of arabidopsis thaliana phosphoserine aminotransferase isoform 1 (atpsat1) in complex with plp- phosphoserine geminal diamine intermediate (see paper)
51% identity, 99% coverage: 2:361/362 of query aligns to 1:359/360 of 6czzA

query
sites
6czzA

A

A

Q

R

V

V

Y

F

N

N

F

F

S

A

G

G

P

P

A

A

M

T

L

L

P

P

A

E

E

N

V

V

L

L

K

L

L

K

A

A

Q

Q

K

A

E

D

L

L

C

Y

D

N

W

W

N

R

G

G

L

S

G

G

T

M

S

S

V

V

M

M

E

E

I

M

S

S

H

H

R

R

G

G

K

K

E

E

F

F

I

L

K

S

V

I

A

I

E

Q

E

K

A

A

E

E

Q

S

D

D

F

L

R

R

S

Q

L

L

N

E

I

I

P

P

S

Q

N

E

Y

Y

K

S

V

V

L

L

F

F

C

L

H

Q

G

G

G
|
G

G
x
A

R
x
T

G

T

Q

Q

F

F

A

A

G

A

I

L

P

P

L

L

N

N

L

L

L

C

G

K

D

S

K

D

T

D

V

T

A

V

D

D

Y

F

V

V

D

V

A

T

G

G

Y

S

W
|
W

A

G

A

D

S

K

A

A

V

V

K

K

E

E

A

A

H

K

K

K

Y

Y

C

C

T

K

P

T

N

N

V

V

I

I

D

W

A

S

K

G

I

K

T

S

V

-

D

E

G

K

L

Y

R

T

A

K

V

V

K

P

P

S

M

F

S

E

E

E

W

L

Q

E

L

Q

T

T

P

P

G

D

A

A

A

K

Y

Y

L

L

H

H

Y

I

C

C

P

A

N

N

E

E

T
|
T

I

I

D

H

G

G

I

V

A

E

I

F

E

K

E

D

A

Y

P

P

S

V

F

P

G

K

D

N

D

G

V

F

V

L

V

V

T

-

A

A

D
|
D

L

M

S
|
S

S

S

T

N

I

F

L

C

S

S

R

K

E

P

I

V

D

D

V

V

S

S

R

K

Y

F

G

G

V

V

I

I

Y

Y

A

G

G

G

A

A

Q
|
Q

K
|
K

N

N

I

V

G

G

P

P

A

S

G

G

L

V

T

T

L

I

I

V

I

I

V

I

R

R

E

K

D

D

L

L

L

I

G

G

K

N

A

A

S

Q

V

D

A

I

C

T

P

P

S

V

I

M

L

L

D

D

Y

Y

S

K

I

I

L

H

S

D

A

E

N

N

D

S

S

S

M

L

F

Y

N
|
N

T
|
T

P

P

T

C

F

F

A

G

W

I

Y

Y

L

M

S

C

G

G

L

L

V

V

F

F

K

E

W

D

L

L

K

L

E

E

Q

Q

G

G

V

L

A

K

A

E

M

V

D

E

K

K

I

K

N

N

Q

Q

Q

R

K

K

A

A

D

D

L

L

Y

Y

S

N

T

A

I

I

D

E

N

E

S

S

-

N

A

G

F

F

Y

F

R

R

N

C

D

P

V

V

A

E

A

K

A

S

N

V

R

R

S

S

R

L

M

M

N

N

V

V

P

P

F

F

Q

T

L

L

A

E

D

K

S

S

A

E

L

L

D

E

K

A

L

E

F

F

L

I

E

K

E

E

S

A

F

A

A

K

A

E

G

K

L

M

H

V

A

Q

L

L

K

K

G

G

H
|
H

R
|
R

V

S

V

V

G

G

M

M

R
|
R

A

A

S

S

I

I

Y

Y

N

N

A

A

M

M

P

P

L

L

A

A

G

G

V

V

K

E

A

K

L

L

T

V

D

A

F

F

M

M

Q

K

D

D

F

F

E

Q

R

A

R

K

H

H

binding pyridoxal-5'-phosphate: G74 (= G75), A75 (≠ G76), T76 (≠ R77), W101 (= W102), T151 (= T153), D171 (= D174), S173 (= S176), Q194 (= Q197), K195 (= K198), N236 (= N239), T237 (= T240)
binding phosphoserine: W101 (= W102), T151 (= T153), K195 (= K198), H326 (= H328), R327 (= R329), R333 (= R335)

Q96255 Phosphoserine aminotransferase 1, chloroplastic; AtPSAT1; Phosphohydroxythreonine aminotransferase; EC 2.6.1.52 from Arabidopsis thaliana (Mouse-ear cress) (see paper)
51% identity, 99% coverage: 3:361/362 of query aligns to 72:429/430 of Q96255

query
sites
Q96255

Q

R

V

V

Y

F

N

N

F

F

S

A

G

G

P

P

A

A

M

T

L

L

P

P

A

E

E

N

V

V

L

L

K

L

L

K

A

A

Q

Q

K

A

E

D

L

L

C

Y

D

N

W

W

N

R

G

G

L

S

G

G

T

M

S

S

V

V

M

M

E

E

I

M

S

S

H

H

R

R

G

G

K

K

E

E

F

F

I

L

K

S

V

I

A

I

E

Q

E

K

A

A

E

E

Q

S

D

D

F

L

R

R

S

Q

L

L

N

E

I

I

P

P

S

Q

N

E

Y

Y

K

S

V

V

L

L

F

F

C

L

H

Q

G

G

G
x
A

R
x
T

G

T

Q

Q

F

F

A

A

G

A

I

L

P

P

L

L

N

N

L

L

L

C

G

K

D

S

K

D

T

D

V

T

A

V

D

D

Y

F

V

V

D

V

A

T

G

G

Y

S

W
|
W

A

G

A

D

S

K

A

A

V

V

K

K

E

E

A

A

H

K

K

K

Y

Y

C

C

T

K

P

T

N

N

V

V

I

I

D

W

A

S

K

G

I

K

T

S

V

-

D

E

G

K

L

Y

R

T

A

K

V

V

K

P

P

S

M

F

S

E

E

E

W

L

Q

E

L

Q

T

T

P

P

G

D

A

A

A

K

Y

Y

L

L

H

H

Y

I

C

C

P

A

N

N

E

E

T
|
T

I

I

D

H

G

G

I

V

A

E

I

F

E

K

E

D

A

Y

P

P

S

V

F

P

G

K

D

N

D

G

V

F

V

L

V

V

T

-

A

A

D
|
D

L

M

S

S

T

N

I

F

L

C

S

S

R

K

E

P

I

V

D

D

V

V

S

S

R

K

Y

F

G

G

V

V

I

I

Y

Y

A

G

G

G

A

A

Q
|
Q

K
|
K

N

N

I

V

G

G

P

P

A

S

G

G

L

V

T

T

L

I

I

V

I

I

V

I

R

R

E

K

D

D

L

L

L

I

G

G

K

N

A

A

S

Q

V

D

A

I

C

T

P

P

S

V

I

M

L

L

D

D

Y

Y

S

K

I

I

L

H

S

D

A

E

N

N

D

S

S

S

M

L

F

Y

N
|
N

T
|
T

P

P

T

C

F

F

A

G

W

I

Y

Y

L

M

S

C

G

G

L

L

V

V

F

F

K

E

W

D

L

L

K

L

E

E

Q

Q

G

G

V

L

A

K

A

E

M

V

D

E

K

K

I

K

N

N

Q

Q

Q

R

K

K

A

A

D

D

L

L

Y

Y

S

N

T

A

I

I

D

E

N

E

S

S

-

N

A

G

F

F

Y

F

R

R

N

C

D

P

V

V

A

E

A

K

A

S

N

V

R

R

S

S

R

L

M

M

N

N

V

V

P

P

F

F

Q

T

L

L

A

E

D

K

S

S

A

E

L

L

D

E

K

A

L

E

F

F

L

I

E

K

E

E

S

A

F

A

A

K

A

E

G

K

L

M

H

V

A

Q

L

L

K

K

G

G

H

H

R

R

V

S

V

V

G

G

M

M

R

R

A

A

S

S

I

I

Y

Y

N

N

A

A

M

M

P

P

L

L

A

A

G

G

V

V

K

E

A

K

L

L

T

V

D

A

F

F

M

M

Q

K

D

D

F

F

E

Q

R

A

R

K

H

H

AT 145:146 (≠ GR 76:77) binding
W171 (= W102) binding
T221 (= T153) binding
D241 (= D174) binding
Q264 (= Q197) binding
K265 (= K198) modified: N6-(pyridoxal phosphate)lysine
NT 306:307 (= NT 239:240) binding

8a5wC Crystal structure of the human phosphoserine aminotransferase (psat) in complex with o-phosphoserine (see paper)
47% identity, 99% coverage: 3:361/362 of query aligns to 1:363/365 of 8a5wC

query
sites
8a5wC

Q

Q

V

V

Y

V

N

N

F

F

S

G

S

P

G

G

P

P

A

A

M

K

L

L

P

P

A

H

E

S

V

V

L

L

K

L

L

E

A

I

Q

Q

K

K

E

E

L

L

C

L

D

D

W

Y

N

K

G

G

L

V

G

G

T

I

S

S

V

V

M

L

E

E

I

M

S

S

H
|
H

R
|
R

G

S

K

S

E

D

F

F

I

A

K

K

V

I

A

I

E

N

A

T

E

E

Q

N

D

L

F

V

R

R

S

E

L

L

N

A

I

V

P

P

S

D

N

N

Y

Y

K

K

V

V

L

I

F

F

C

L

H

Q

G

G

R

C

G

G

Q

Q

F

F

A

S

G

A

I

V

P

P

L

L

N

N

L

L

L

I

G

G

D

L

K

K

T

A

-

G

-

R

V

C

A

A

D

D

Y

Y

V

V

D

V

A

T

G

G

Y

A

W

W

A

S

A

A

S

K

A

A

V

A

K

E

E

E

A

A

H

K

K

K

Y

F

C

G

T

T

P

I

N

N

V

I

I

V

D

H

A

P

K

K

I

L

T

-

V

-

D

G

G

S

L

Y

R

T

A

K

V

I

K

P

P

D

M

P

S

S

E

T

W

W

Q

N

L

L

T

N

P

P

G

D

A

A

A

S

Y

Y

L

V

H

Y

Y

Y

C

C

P

A

N

N

E

E

T
|
T

I

V

D

H

G

G

I

V

A

E

I

F

E

D

E

F

A

I

P

P

S

D

F

V

G

K

D

G

D

A

V

V

V

L

V

V

T

-

A

C

D

D

L

M

S

S

T

N

I

F

L

L

S

S

R

K

E

P

I

V

D

D

V

V

S

S

R

K

Y

F

G

G

V

V

I

I

Y

F

A

A

G

G

A

A

Q

Q

K
|
K

N

N

I

V

G

G

P

S

A

A

G

G

L

V

T

T

L

V

I

V

I

I

V

V

R

R

E

D

D

D

L

L

G

G

K

F

A

A

S

L

V

R

A

E

C

C

P

P

S

S

I

V

L

L

D

E

Y

Y

S

K

I

V

L

Q

S

A

A

G

N

N

D

S

S

S

M

L

F

Y

N
|
N

T
|
T

P

P

T

C

F

F

A

S

W

I

Y

Y

L

V

S

M

G

G

L

L

V

V

F

L

K

E

W

W

L

I

K

K

E

N

Q

N

G

G

V

A

A

A

M

M

D

E

K

K

I

L

N

S

Q

S

Q

I

K

K

A

S

D

Q

L

T

L

I

Y

Y

S

E

T

I

I

I

D

D

N

N

S

S

-

Q

A

G

F

F

Y

Y

R

V

N

C

D

P

V

V

A

E

A

P

A

Q

N

N

R

R

S

S

R

K

M

M

N

N

V

I

P

P

F

F

Q

R

L

I

-

G

-

N

-

A

-

K

A

G

D

D

S

D

A

A

L

L

D

E

K

K

L

R

F

F

L

L

E

D

E

K

S

A

F

L

A

E

A

L

G

N

L

M

H

L

A

S

L

L

K

K

G

G

H
|
H

R
|
R

V

S

V

V

G

G

M

I

R
|
R

A

A

S

S

I

L

Y

Y

N

N

A

A

M

V

P

T

L

I

A

E

G

D

V

V

K

Q

A

K

L

L

T

A

D

A

F

F

M

M

Q

K

D

K

F

F

E

L

R

E

R

M

H

H

binding (2S)-2-[(E)-[2-methyl-3-oxidanyl-5-(phosphonooxymethyl)pyridin-4-yl]methylideneamino]-3-phosphonooxy-propanoic acid: H39 (= H41), R40 (= R42), N236 (= N239), T237 (= T240)
binding phosphoserine: T151 (= T153), K195 (= K198), H330 (= H328), R331 (= R329), R337 (= R335)

8a5wA Crystal structure of the human phosphoserine aminotransferase (psat) in complex with o-phosphoserine (see paper)
47% identity, 99% coverage: 3:361/362 of query aligns to 1:363/365 of 8a5wA

query
sites
8a5wA

Q

Q

V

V

Y

V

N

N

F

F

S

G

S

P

G

G

P

P

A

A

M

K

L

L

P

P

A

H

E

S

V

V

L

L

K

L

L

E

A

I

Q

Q

K

K

E

E

L

L

C

L

D

D

W

Y

N

K

G

G

L

V

G

G

T

I

S

S

V

V

M

L

E

E

I

M

S

S

H
|
H

R
|
R

G

S

K

S

E

D

F

F

I

A

K

K

V

I

A

I

E

N

A

T

E

E

Q

N

D

L

F

V

R

R

S

E

L

L

N

A

I

V

P

P

S

D

N

N

Y

Y

K

K

V

V

L

I

F

F

C

L

H

Q

G

G

G
|
G

R
x
C

G

G

Q

Q

F

F

A

S

G

A

I

V

P

P

L

L

N

N

L

L

L

I

G

G

D

L

K

K

T

A

-

G

-

R

V

C

A

A

D

D

Y

Y

V

V

D

V

A

T

G

G

Y

A

W
|
W

A

S

A

A

S

K

A

A

V

A

K

E

E

E

A

A

H

K

K

K

Y

F

C

G

T

T

P

I

N

N

V

I

I

V

D

H

A

P

K

K

I

L

T

-

V

-

D

G

G

S

L

Y

R

T

A

K

V

I

K

P

P

D

M

P

S

S

E

T

W

W

Q

N

L

L

T

N

P

P

G

D

A

A

A

S

Y

Y

L

V

H

Y

Y

Y

C

C

P

A

N

N

E

E

T
|
T

I

V

D

H

G

G

I

V

A

E

I

F

E

D

E

F

A

I

P

P

S

D

F

V

G

K

D

G

D

A

V

V

V

L

V

V

T

-

A

C

D
|
D

L

M

S
|
S

S

S

T

N

I

F

L

L

S

S

R

K

E

P

I

V

D

D

V

V

S

S

R

K

Y

F

G

G

V

V

I

I

Y

F

A

A

G

G

A

A

Q
|
Q

K
|
K

N

N

I

V

G

G

P

S

A

A

G

G

L

V

T

T

L

V

I

V

I

I

V

V

R

R

E

D

D

D

L

L

G

G

K

F

A

A

S

L

V

R

A

E

C

C

P

P

S

S

I

V

L

L

D

E

Y

Y

S

K

I

V

L

Q

S

A

A

G

N

N

D

S

S

S

M

L

F

Y

N

N

T

T

P

P

T

C

F

F

A

S

W

I

Y

Y

L

V

S

M

G

G

L

L

V

V

F

L

K

E

W

W

L

I

K

K

E

N

Q

N

G

G

V

A

A

A

M

M

D

E

K

K

I

L

N

S

Q

S

Q

I

K

K

A

S

D

Q

L

T

L

I

Y

Y

S

E

T

I

I

I

D

D

N

N

S

S

-

Q

A

G

F

F

Y

Y

R

V

N

C

D

P

V

V

A

E

A

P

A

Q

N

N

R

R

S

S

R

K

M

M

N

N

V

I

P

P

F

F

Q

R

L

I

-

G

-

N

-

A

-

K

A

G

D

D

S

D

A

A

L

L

D

E

K

K

L

R

F

F

L

L

E

D

E

K

S

A

F

L

A

E

A

L

G

N

L

M

H

L

A

S

L

L

K

K

G

G

H
|
H

R

R

V

S

V

V

G

G

M

I

R
|
R

A

A

S

S

I

L

Y

Y

N

N

A

A

M

V

P

T

L

I

A

E

G

D

V

V

K

Q

A

K

L

L

T

A

D

A

F

F

M

M

Q

K

D

K

F

F

E

L

R

E

R

M

H

H

binding 4'-deoxy-4'-aminopyridoxal-5'-phosphate: G73 (= G75), G74 (= G76), C75 (≠ R77), W102 (= W102), T151 (= T153), D171 (= D174), S173 (= S176), Q194 (= Q197), K195 (= K198)
binding phosphoserine: H39 (= H41), R40 (= R42), H330 (= H328), R337 (= R335)

8a5vA Crystal structure of the human phosposerine aminotransferase (psat) (see paper)
47% identity, 99% coverage: 3:361/362 of query aligns to 1:363/365 of 8a5vA

query
sites
8a5vA

Q

Q

V

V

Y

V

N

N

F

F

S

G

S

P

G

G

P

P

A

A

M

K

L

L

P

P

A

H

E

S

V

V

L

L

K

L

L

E

A

I

Q

Q

K

K

E

E

L

L

C

L

D

D

W

Y

N

K

G

G

L

V

G

G

T

I

S

S

V

V

M

L

E

E

I

M

S

S

H

H

R

R

G

S

K

S

E

D

F

F

I

A

K

K

V

I

A

I

E

N

A

T

E

E

Q

N

D

L

F

V

R

R

S

E

L

L

N

A

I

V

P

P

S

D

N

N

Y

Y

K

K

V

V

L

I

F

F

C

L

H

Q

G

G

R

C

G

G

Q

Q

F

F

A

S

G

A

I

V

P

P

L

L

N

N

L

L

L

I

G

G

D

L

K

K

T

A

-

G

-

R

V

C

A

A

D

D

Y

Y

V

V

D

V

A

T

G

G

Y

A

W

W

A

S

A

A

S

K

A

A

V

A

K

E

E

E

A

A

H

K

K

K

Y

F

C

G

T

T

P

I

N

N

V

I

I

V

D

H

A

P

K

K

I

L

T

-

V

-

D

G

G

S

L

Y

R

T

A

K

V

I

K

P

P

D

M

P

S

S

E

T

W

W

Q

N

L

L

T

N

P

P

G

D

A

A

A

S

Y

Y

L

V

H

Y

Y

Y

C

C

P

A

N

N

E

E

T

T

I

V

D

H

G

G

I

V

A

E

I

F

E

D

E

F

A

I

P

P

S

D

F

V

G

K

D

G

D

A

V

V

V

L

V

V

T

-

A

C

D

D

L

M

S

S

T

N

I

F

L

L

S

S

R

K

E

P

I

V

D

D

V

V

S

S

R

K

Y

F

G

G

V

V

I

I

Y

F

A

A

G

G

A

A

Q

Q

K

K

N

N

I

V

G

G

P

S

A

A

G

G

L

V

T

T

L

V

I

V

I

I

V

V

R

R

E

D

D

D

L

L

G

G

K

F

A

A

S

L

V

R

A

E

C

C

P

P

S

S

I

V

L

L

D

E

Y

Y

S

K

I

V

L

Q

S

A

A

G

N

N

D

S

S

S

M

L

F

Y

N
|
N

T
|
T

P

P

T

C

F

F

A

S

W

I

Y

Y

L

V

S

M

G

G

L

L

V

V

F

L

K

E

W

W

L

I

K

K

E

N

Q

N

G

G

V

A

A

A

M

M

D

E

K

K

I

L

N

S

Q

S

Q

I

K

K

A

S

D

Q

L

T

L

I

Y

Y

S

E

T

I

I

I

D

D

N

N

S

S

-

Q

A

G

F

F

Y

Y

R

V

N

C

D

P

V

V

A

E

A

P

A

Q

N

N

R

R

S

S

R

K

M

M

N

N

V

I

P

P

F

F

Q

R

L

I

-

G

-

N

-

A

-

K

A

G

D

D

S

D

A

A

L

L

D

E

K

K

L

R

F

F

L

L

E

D

E

K

S

A

F

L

A

E

A

L

G

N

L

M

H

L

A

S

L

L

K

K

G

G

H

H

R

R

V

S

V

V

G

G

M

I

R

R

A

A

S

S

I

L

Y

Y

N

N

A

A

M

V

P

T

L

I

A

E

G

D

V

V

K

Q

A

K

L

L

T

A

D

A

F

F

M

M

Q

K

D

K

F

F

E

L

R

E

R

M

H

H

binding 4'-deoxy-4'-aminopyridoxal-5'-phosphate: N236 (= N239), T237 (= T240)

8a5vE Crystal structure of the human phosposerine aminotransferase (psat) (see paper)
47% identity, 99% coverage: 3:361/362 of query aligns to 2:364/366 of 8a5vE

query
sites
8a5vE

Q

Q

V

V

Y

V

N

N

F

F

S

G

S

P

G

G

P

P

A

A

M

K

L

L

P

P

A

H

E

S

V

V

L

L

K

L

L

E

A

I

Q

Q

K

K

E

E

L

L

C

L

D

D

W

Y

N

K

G

G

L

V

G

G

T

I

S

S

V

V

M

L

E

E

I

M

S

S

H

H

R

R

G

S

K

S

E

D

F

F

I

A

K

K

V

I

A

I

E

N

A

T

E

E

Q

N

D

L

F

V

R

R

S

E

L

L

N

A

I

V

P

P

S

D

N

N

Y

Y

K

K

V

V

L

I

F

F

C

L

H

Q

G

G

R

C

G

G

Q

Q

F

F

A

S

G

A

I

V

P

P

L

L

N

N

L

L

L

I

G

G

D

L

K

K

T

A

-

G

-

R

V

C

A

A

D

D

Y

Y

V

V

D

V

A

T

G

G

Y

A

W
|
W

A

S

A

A

S

K

A

A

V

A

K

E

E

E

A

A

H

K

K

K

Y

F

C

G

T

T

P

I

N

N

V

I

I

V

D

H

A

P

K

K

I

L

T

-

V

-

D

G

G

S

L

Y

R

T

A

K

V

I

K

P

P

D

M

P

S

S

E

T

W

W

Q

N

L

L

T

N

P

P

G

D

A

A

A

S

Y

Y

L

V

H

Y

Y

Y

C

C

P

A

N

N

E

E

T

T

I

V

D

H

G

G

I

V

A

E

I

F

E

D

E

F

A

I

P

P

S

D

F

V

G

K

D

G

D

A

V

V

V

L

V

V

T

-

A

C

D

D

L

M

S

S

T

N

I

F

L

L

S

S

R

K

E

P

I

V

D

D

V

V

S

S

R

K

Y

F

G

G

V

V

I

I

Y

F

A

A

G

G

A

A

Q

Q

K

K

N

N

I

V

G

G

P

S

A

A

G

G

L

V

T

T

L

V

I

V

I

I

V

V

R

R

E

D

D

D

L

L

G

G

K

F

A

A

S

L

V

R

A

E

C

C

P

P

S

S

I

V

L

L

D

E

Y

Y

S

K

I

V

L

Q

S

A

A

G

N

N

D

S

S

S

M

L

F

Y

N

N

T

T

P

P

T

C

F

F

A

S

W

I

Y

Y

L

V

S

M

G

G

L

L

V

V

F

L

K

E

W

W

L

I

K

K

E

N

Q

N

G

G

V

A

A

A

M

M

D

E

K

K

I

L

N

S

Q

S

Q

I

K

K

A

S

D

Q

L

T

L

I

Y

Y

S

E

T

I

I

I

D

D

N

N

S

S

-

Q

A

G

F

F

Y

Y

R

V

N

C

D

P

V

V

A

E

A

P

A

Q

N

N

R

R

S

S

R

K

M

M

N

N

V

I

P

P

F

F

Q

R

L

I

-

G

-

N

-

A

-

K

A

G

D

D

S

D

A

A

L

L

D

E

K

K

L

R

F

F

L

L

E

D

E

K

S

A

F

L

A

E

A

L

G

N

L

M

H

L

A

S

L

L

K

K

G

G

H

H

R
|
R

V

S

V

V

G

G

M

I

R

R

A

A

S

S

I

L

Y

Y

N

N

A

A

M

V

P

T

L

I

A

E

G

D

V

V

K

Q

A

K

L

L

T

A

D

A

F

F

M

M

Q

K

D

K

F

F

E

L

R

E

R

M

H

H

binding sulfate ion: W103 (= W102), R332 (= R329)

Q9Y617 Phosphoserine aminotransferase; Phosphohydroxythreonine aminotransferase; PSAT; EC 2.6.1.52 from Homo sapiens (Human) (see 6 papers)
47% identity, 99% coverage: 3:361/362 of query aligns to 6:368/370 of Q9Y617

query
sites
Q9Y617

Q

Q

V

V

Y

V

N

N

F

F

S

G

S

P

G

G

P

P

A

A

M

K

L

L

P

P

A

H

E

S

V

V

L

L

K

L

L

E

A

I

Q

Q

K

K

E

E

L

L

C

L

D

D

W

Y

N

K

G

G

L

V

G

G

T

I

S

S

V

V

M

L

E

E

I

M

S
|
S

H
|
H

R
|
R

G

S

K

S

E

D

F

F

I

A

K

K

V

I

A

I

E

N

A

T

E

E

Q

N

D

L

F

V

R

R

S

E

L

L

N

A

I

V

P

P

S

D

N

N

Y
|
Y

K

K

V

V

L

I

F

F

C

L

H

Q

G

G

G
|
G

R
x
C

G

G

Q

Q

F

F

A

S

G

A

I

V

P
|
P

L

L

N

N

L

L

L

I

G

G

D

L

K

K

T

A

-

G

-

R

V

C

A
|
A

D
|
D

Y

Y

V

V

D

V

A

T

G

G

Y

A

W
|
W

A

S

A

A

S

K

A

A

V

A

K

E

E

E

A

A

H

K

K

K

Y

F

C

G

T

T

P

I

N

N

V

I

I

V

D

H

A

P

K

K

I

L

T

-

V

-

D

G

G

S

L

Y

R

T

A

K

V

I

K

P

P

D

M

P

S

S

E

T

W

W

Q

N

L

L

T

N

P

P

G

D

A

A

A

S

Y

Y

L

V

H

Y

Y

Y

C

C

P

A

N

N

E
|
E

T
|
T

I

V

D

H

G

G

I

V

A

E

I

F

E

D

E

F

A

I

P

P

S

D

F

V

G

K

D

G

D

A

V

V

V

L

V

V

T

-

A

C

D
|
D

L

M

S

S

S
|
S

T

N

I

F

L

L

S

S

R

K

E

P

I

V

D

D

V

V

S

S

R

K

Y

F

G

G

V

V

I

I

Y

F

A

A

G

G

A

A

Q
|
Q

K
|
K

N

N

I

V

G

G

P

S

A

A

G

G

L

V

T

T

L

V

I

V

I

I

V

V

R

R

E

D

D

D

L

L

G

G

K

F

A

A

S

L

V

R

A

E

C

C

P

P

S

S

I

V

L

L

D

E

Y

Y

S

K

I

V

L

Q

S

A

A

G

N

N

D

S

S

S

M

L

F

Y

N
|
N

T
|
T

P

P

T
x
C

F

F

A

S

W

I

Y

Y

L

V

S

M

G

G

L

L

V

V

F

L

K

E

W

W

L

I

K

K

E

N

Q

N

G

G

V

A

A

A

M

M

D

E

K

K

I

L

N

S

Q

S

Q

I

K

K

A

S

D

Q

L

T

L

I

Y

Y

S

E

T

I

I

I

D

D

N

N

S

S

-

Q

A

G

F

F

Y

Y

R

V

N

C

D

P

V

V

A

E

A

P

A

Q

N

N

R

R

S

S

R

K

M

M

N

N

V

I

P

P

F

F

Q

R

L

I

-

G

-

N

-

A

-

K

A

G

D

D

S

D

A

A

L

L

D

E

K

K

L

R

F

F

L

L

E

D

E

K

S

A

F

L

A

E

A

L

G

N

L

M

H

L

A

S

L

L

K

K

G

G

H
|
H

R
|
R

V

S

V

V

G

G

M

I

R
|
R

A

A

S

S

I

L

Y

Y

N

N

A

A

M

V

P

T

L

I

A

E

G

D

V

V

K

Q

A

K

L

L

T

A

D

A

F

F

M

M

Q

K

D

K

F

F

E

L

R

E

R

M

H

H

S43 (= S40) to R: in PSATD; reduced O-phospho-L-serine:2-oxoglutarate aminotransferase catalytic efficiency; 3-fold increase of KM for 3-phosphohydroxypyruvate; 5-fold increase of KM for L-glutamate; decreased function in L-serine biosynthesis shown through in vitro reconstruction of the phosphorylated pathway; does not affect secondary structure; does not affect dimerization; does not affect thermal stability
H44 (= H41) binding in other chain
R45 (= R42) binding in other chain
Y70 (= Y67) to N: in NLS2; unknown pathological significance
G79 (= G76) binding ; to W: in NLS2; loss of O-phospho-L-serine:2-oxoglutarate aminotransferase activity; loss of function in L-serine biosynthesis shown through in vitro reconstruction of the phosphorylated pathway
C80 (≠ R77) binding
P87 (= P84) to A: has no effect on O-phospho-L-serine:2-oxoglutarate aminotransferase catalytic efficiency; does not affect KM for 3-phosphohydroxypyruvate; does not affect KM for L-glutamate; no effect on function in L-serine biosynthesis shown through in vitro reconstruction of the phosphorylated pathway; does not affect secondary structure; does not affect dimerization; does not affect thermal stability; dbSNP:rs11540974
A99 (= A94) to V: in NLS2; does not affect secondary structure; does not affect dimerization; increased thermal stability; dbSNP:rs587777778
D100 (= D95) to A: in PSATD; has no effect on O-phospho-L-serine:2-oxoglutarate aminotransferase catalytic efficiency; does not affect KM for 3-phosphohydroxypyruvate; does not affect KM for L-glutamate; does not affect secondary structure; results in increased protein aggregation as shown by dynamic light scattering; dbSNP:rs118203967
W107 (= W102) binding
E155 (= E152) to Q: in NLS2; unknown pathological significance
T156 (= T153) binding
D176 (= D174) binding
S179 (= S177) to L: in NLS2; loss of O-phospho-L-serine:2-oxoglutarate aminotransferase activity; loss of function in L-serine biosynthesis shown through in vitro reconstruction of the phosphorylated pathway; dbSNP:rs587777777
Q199 (= Q197) binding
K200 (= K198) modified: N6-(pyridoxal phosphate)lysine
N241 (= N239) binding in other chain
T242 (= T240) binding in other chain
C245 (≠ T243) to R: in NLS2; reduced O-phospho-L-serine:2-oxoglutarate aminotransferase catalytic efficiency; 9-fold increase of KM for L-glutamate; does not affect KM for 3-phosphohydroxypyruvate; decreased function in L-serine biosynthesis shown through in vitro reconstruction of the phosphorylated pathway; does not affect secondary structure; does not affect dimerization
H335 (= H328) binding
R336 (= R329) binding
R342 (= R335) binding ; to W: in NLS2; loss of O-phospho-L-serine:2-oxoglutarate aminotransferase activity; loss of function in L-serine biosynthesis shown through in vitro reconstruction of the phosphorylated pathway; dbSNP:rs202103028

8a5wE Crystal structure of the human phosphoserine aminotransferase (psat) in complex with o-phosphoserine (see paper)
47% identity, 99% coverage: 3:361/362 of query aligns to 2:363/365 of 8a5wE

query
sites
8a5wE

Q

Q

V

V

Y

V

N

N

F

F

S

G

S

P

G

G

P

P

A

A

M

K

L

L

P

P

A

H

E

S

V

V

L

L

K

L

L

E

A

I

Q

Q

K

K

E

E

L

L

C

L

D

D

W

Y

N

K

G

G

L

V

G

G

T

I

S

S

V

V

M

L

E

E

I

M

S

S

H
|
H

R
|
R

G

S

K

S

E

D

F

F

I

A

K

K

V

I

A

I

E

N

A

T

E

E

Q

N

D

L

F

V

R

R

S

E

L

L

N

A

I

V

P

P

S

D

N

N

Y

Y

K

K

V

V

L

I

F

F

C

L

H

Q

G

G

G
|
G

R
x
C

G

G

Q

Q

F

F

A

S

G

A

I

V

P

P

L

L

N

N

L

L

L

I

G

G

D

L

K

K

T

A

-

G

-

R

V

C

A

A

D

D

Y

Y

V

V

D

V

A

T

G

G

Y

A

W
|
W

A

S

A

A

S

K

A

A

V

A

K

E

E

E

A

A

H

K

K

K

Y

F

C

G

T

T

P

I

N

N

V

I

I

V

D

H

A

P

K

K

I

L

T

-

V

-

D

G

G

S

L

Y

R

T

A

K

V

I

K

P

P

D

M

P

S

S

E

T

W

W

Q

N

L

L

T

N

P

P

G

D

A

A

A

S

Y

Y

L

V

H

Y

Y

Y

C

C

P

A

N

N

E

E

T
|
T

I

V

D

H

G

G

I

V

A

E

I

F

E

D

E

F

A

I

P

P

S

D

F

V

G

K

D

G

D

A

V

V

V

L

V

V

T

-

A

C

D

D

L

M

S
|
S

S

S

T

N

I

F

L

L

S

S

R

K

E

P

I

V

D

D

V

V

S

S

R

K

Y

F

G

G

V

V

I

I

Y

F

A

A

G

G

A
|
A

Q
|
Q

K

-

N
|
N

I

V

G

G

P

S

A

A

G

G

L

V

T

T

L

V

I

V

I

I

V

V

R

R

E

D

D

D

L

L

G

G

K

F

A

A

S

L

V

R

A

E

C

C

P

P

S

S

I

V

L

L

D

E

Y

Y

S

K

I

V

L

Q

S

A

A

G

N

N

D

S

S

S

M

L

F

Y

N
|
N

T
|
T

P

P

T

C

F

F

A

S

W

I

Y

Y

L

V

S

M

G

G

L

L

V

V

F

L

K

E

W

W

L

I

K

K

E

N

Q

N

G

G

V

A

A

A

M

M

D

E

K

K

I

L

N

S

Q

S

Q

I

K

K

A

S

D

Q

L

T

L

I

Y

Y

S

E

T

I

I

I

D

D

N

N

S

S

-

Q

A

G

F

F

Y

Y

R

V

N

C

D

P

V

V

A

E

A

P

A

Q

N

N

R

R

S

S

R

K

M

M

N

N

V

I

P

P

F

F

Q

R

L

I

-

G

-

N

-

A

-

K

A

G

D

D

S

D

A

A

L

L

D

E

K

K

L

R

F

F

L

L

E

D

E

K

S

A

F

L

A

E

A

L

G

N

L

M

H

L

A

S

L

L

K

K

G

G

H
|
H

R
|
R

V

S

V

V

G

G

M

I

R
|
R

A

A

S

S

I

L

Y
|
Y

N

N

A

A

M

V

P

T

L

I

A

E

G

D

V

V

K

Q

A

K

L

L

T

A

D

A

F

F

M

M

Q

K

D

K

F

F

E

L

R

E

R

M

H

H

binding (2S)-2-[(E)-[2-methyl-3-oxidanyl-5-(phosphonooxymethyl)pyridin-4-yl]methylideneamino]-3-phosphonooxy-propanoic acid: H40 (= H41), R41 (= R42), N236 (= N239), T237 (= T240)
binding (2~{S})-2-[[(~{R})-[[(5~{S})-5-azanyl-6-oxidanylidene-hexyl]amino]-[2-methyl-3-oxidanyl-5-(phosphonooxymethyl)pyridin-4-yl]methyl]amino]-3-phosphonooxy-propanoic acid: G74 (= G75), G75 (= G76), C76 (≠ R77), W103 (= W102), T152 (= T153), S174 (= S176), A194 (= A196), Q195 (= Q197), N196 (= N199), H330 (= H328), R331 (= R329), R337 (= R335), Y341 (= Y339)

3e77A Human phosphoserine aminotransferase in complex with plp
46% identity, 97% coverage: 12:361/362 of query aligns to 8:361/363 of 3e77A

query
sites
3e77A

A

S

M

M

L

L

P

P

A

H

E

S

V

V

L

L

K

L

L

E

A

I

Q

Q

K

K

E

E

L

L

C

L

D

D

W

Y

N

K

G

G

L

V

G

G

T

I

S

S

V

V

M

L

E

E

I

M

S

S

H

H

R

R

G

S

K

S

E

D

F

F

I

A

K

K

V

I

A

I

E

N

A

T

E

E

Q

N

D

L

F

V

R

R

S

E

L

L

N

A

I

V

P

P

S

D

N

N

Y

Y

K

K

V

V

L

I

F

F

C

L

H

Q

G

G

G
|
G

R
x
C

G

G

Q

Q

F

F

A

S

G

A

I

V

P

P

L

L

N

N

L

L

L

I

G

G

D

L

K

K

T

A

-

G

-

R

V

C

A

A

D

D

Y

Y

V

V

D

V

A

T

G

G

Y

A

W
|
W

A

S

A

A

S

K

A

A

V

A

K

E

E

E

A

A

H

K

K

K

Y

F

C

G

T

T

P

I

N

N

V

I

I

V

D

H

A

P

K

K

I

L

T

-

V

-

D

G

G

S

L

Y

R

T

A

K

V

I

K

P

P

D

M

P

S

S

E

T

W

W

Q

N

L

L

T

N

P

P

G

D

A

A

A

S

Y

Y

L

V

H

Y

Y

Y

C

C

P

A

N

N

E

E

T
|
T

I

V

D

H

G

G

I

V

A

E

I

F

E

D

E

F

A

I

P

P

S

D

F

V

G

K

D

G

D

A

V

V

V

L

V

V

T

-

A

C

D
|
D

L

M

S
|
S

S

S

T

N

I

F

L

L

S

S

R

K

E

P

I

V

D

D

V

V

S

S

R

K

Y

F

G

G

V

V

I

I

Y

F

A

A

G

G

A

A

Q
|
Q

K
|
K

N

N

I

V

G

G

P

S

A

A

G

G

L

V

T

T

L

V

I

V

I

I

V

V

R

R

E

D

D

D

L

L

G

G

K

F

A

A

S

L

V

R

A

E

C

C

P

P

S

S

I

V

L

L

D

E

Y

Y

S

K

I

V

L

Q

S

A

A

G

N

N

D

S

S

S

M

L

F

Y

N
|
N

T
|
T

P

P

T

C

F

F

A

S

W

I

Y

Y

L

V

S

M

G

G

L

L

V

V

F

L

K

E

W

W

L

I

K

K

E

N

Q

N

G

G

V

A

A

A

M

M

D

E

K

K

I

L

N

S

Q

S

Q

I

K

K

A

S

D

Q

L

T

L

I

Y

Y

S

E

T

I

I

I

D

D

N

N

S

S

-

Q

A

G

F

F

Y

Y

R

V

N

C

D

P

V

V

A

E

A

P

A

Q

N

N

R

R

S

S

R

K

M

M

N

N

V

I

P

P

F

F

Q

R

L

I

-

G

-

N

-

A

-

K

A

G

D

D

S

D

A

A

L

L

D

E

K

K

L

R

F

F

L

L

E

D

E

K

S

A

F

L

A

E

A

L

G

N

L

M

H

L

A

S

L

L

K

K

G

G

H

H

R

R

V

S

V

V

G

G

M

I

R

R

A

A

S

S

I

L

Y

Y

N

N

A

A

M

V

P

T

L

I

A

E

G

D

V

V

K

Q

A

K

L

L

T

A

D

A

F

F

M

M

Q

K

D

K

F

F

E

L

R

E

R

M

H

H

active site: W100 (= W102), D169 (= D174), K193 (= K198)
binding pyridoxal-5'-phosphate: G71 (= G75), G72 (= G76), C73 (≠ R77), W100 (= W102), T149 (= T153), D169 (= D174), S171 (= S176), Q192 (= Q197), K193 (= K198), N234 (= N239), T235 (= T240)

1bt4A Phosphoserine aminotransferase from bacillus circulans subsp. Alkalophilus
45% identity, 99% coverage: 3:362/362 of query aligns to 3:361/361 of 1bt4A

query
sites
1bt4A

Q

R

V

A

Y

Y

N

N

F

F

S

N

S

A

G

G

P

P

A

A

M

A

L

L

P

P

A

L

E

E

V

V

L

L

K

E

L

R

A

A

Q

Q

K

A

E

E

L

F

C

V

D

D

W

Y

N

Q

G

H

L

T

G

G

T

M

S

S

V

I

M

M

E

E

I

M

S

S

H

H

R

R

G

G

K

A

E

V

F

Y

I

E

K

A

V

V

A

H

E

N

E

E

A

A

E

Q

Q

A

D

R

F

L

R

L

S

A

L

L

N

G

I

N

P

P

S

T

N

G

Y

Y

K

K

V

V

L

L

F

F

C

I

H

Q

G

G

G
x
A

R
x
S

G

T

Q

Q

F

F

A

A

G

M

I

I

P

P

L

M

N

N

L

F

L

L

G

K

D

E

K

G

T

Q

V

T

A

A

D

N

Y

Y

V

V

D

M

A

T

G

G

Y

S

W
|
W

A

A

A

S

S

K

A

A

V

L

K

K

E

E

A

A

H

-

K

K

Y

L

C

I

T

G

P

D

N

T

V

H

I

V

D

A

A

A

K

S

I

S

T

E

V

A

D

S

G

N

L

Y

R

M

A

T

V

L

K

P

P

K

M

L

S

Q

E

E

W

I

Q

Q

L

L

T

Q

P

D

G

N

A

A

Y

Y

L

L

H

H

Y

L

C

T

P

S

N

N

E

E

T

T

I

I

D

E

G

G

I

A

A

Q

I

F

E

K

E

A

A

F

P

P

S

D

F

T

G

G

D

S

D

-

V

V

V

P

V

L

T

I

A

G

D
|
D

L

M

S
|
S

S

S

T

D

I

I

L

L

S

S

R

R

E

P

I

F

D

D

V

L

S

N

R

Q

Y

F

G

G

V

L

I

V

Y

Y

A

A

G

G

A

A

Q
|
Q

K
|
K

N

N

I

L

G

G

P

P

A

S

G

G

L

V

T

T

L

V

I

V

I

I

V

V

R

R

E

E

D

D

L

L

L

V

G

A

K

E

A

S

S

P

V

K

A

H

C

L

P

P

S

T

I

M

L

L

D

R

Y

Y

S

D

I

T

L

Y

S

V

A

K

N

N

D

N

S

S

M

L

F

Y

N

N

T

T

P

P

T

S

F

F

A

G

W

I

Y

Y

L

M

S

V

G

N

L

E

V

V

F

L

K

K

W

W

L

I

K

E

E

E

Q

R

G

G

V

L

A

E

A

G

M

V

D

Q

K

Q

I

A

N

N

Q

R

Q

K

K

K

A

A

D

S

L

L

L

I

Y

Y

S

D

T

A

I

I

D

D

N

Q

S

S

-

G

A

G

F

F

Y

Y

R

R

N

G

D

C

V

V

A

D

A

V

A

D

N

S

R

R

S

S

R

D

M

M

N

N

V

I

P

T

F

F

Q

R

L

L

A

A

D

S

S

E

A

E

L

L

D

E

K

K

L

E

F

F

L

V

E

K

E

A

S

S

F

E

A

Q

A

E

G

G

L

F

H

V

A

G

L

L

K

K

G

G

H

H

R

R

V

S

V

V

G

G

M

L

R

R

A

A

S

S

I

I

Y

Y

N

N

A

A

M

V

P

P

L

Y

A

E

G

S

V

C

K

E

A

A

L

L

T

V

D

Q

F

F

M

M

Q

E

D

H

F

F

E

K

R

R

R

S

H

R

G

G

active site: W102 (= W102), D172 (= D174), K196 (= K198)
binding pyridoxal-5'-phosphate: A76 (≠ G76), S77 (≠ R77), W102 (= W102), D172 (= D174), S174 (= S176), Q195 (= Q197), K196 (= K198)

4azjA Structural basis of l-phosphoserine binding to bacillus alcalophilus phosphoserine aminotransferase (see paper)
42% identity, 99% coverage: 1:359/362 of query aligns to 1:358/360 of 4azjA

query
sites
4azjA

M

V

A

K

Q

Q

V

V

Y

F

N

N

F

F

S

N

S

A

G

G

P

P

A

S

M

A

L

L

P

P

A

K

E

P

V

A

L

L

K

E

L

R

A

A

Q

Q

K

K

E

E

L

L

C

L

D

N

W

F

N

N

G

D

L

T

G

Q

T

M

S

S

V

V

M

M

E

E

I

L

S

S

H
|
H

R
|
R

G

S

K

Q

E

S

F

Y

I

E

K

E

V

V

A

H

E

E

E

Q

A

A

E

Q

Q

N

D

L

F

L

R

R

S

E

L

L

N

Q

I

I

P

P

S

N

N

D

Y

Y

K

Q

V

I

L

L

F

F

C

L

H

Q

G

G

G
x
A

R
x
S

G

L

Q

Q

F

F

A

T

G

M

I

L

P

P

L

M

N

N

L

L

G

T

D

K

K

G

T

T

V

I

A

G

D

N

Y

Y

V

V

D

L

A

T

G

G

Y

S

W
|
W

A

S

A

E

S

K

A

A

V

L

K

K

E

E

A

A

H

-

K

K

Y

L

C

L

T

G

P

E

N

T

V

H

I

I

D

A

A

A

K

S

I

T

T

K

V

A

D

N

G

S

L

Y

R

Q

A

S

V

I

K

P

P

D

M

F

S

S

E

E

W

F

Q

Q

L

L

T

N

P

E

G

N

A

D

A

A

Y

Y

L

L

H

H

Y

I

C

T

P

S

N

N

E

N

T
|
T

I

I

D

Y

G

G

I

T

A

Q

I

Y

E

Q

E

N

A

F

P

P

S

E

F

I

G

N

D

H

D

A

V

P

V

L

V

I

T

-

A

A

D
|
D

L

M

S
|
S

S

S

T

D

I

I

L

L

S

S

R

R

E

P

I

L

D

K

V

V

S

N

R

Q

Y

F

G

G

V

M

I

I

Y

Y

A

A

G

G

A

A

Q
|
Q

K
|
K

N

N

I

L

G

G

P

P

A

S

G

G

L

V

T

T

L

V

I

V

I

I

V

V

R

K

E

K

D

D

L

L

G

N

K

T

A

K

S

V

V

E

A

Q

C

V

P

P

S

T

I

M

L

L

D

Q

Y

Y

S

A

I

T

L

H

S

I

A

K

N

S

D

D

S

S

M

L

F

Y

N
|
N

T
|
T

P

P

T

T

F

F

A

S

W

I

Y

Y

L

M

S

L

G

R

L

N

V

V

F

L

K

D

W

W

L

I

K

K

E

D

Q

L

G

G

V

A

A

E

A

A

M

I

D

A

K

K

I

Q

N

N

Q

E

K

K

A

A

D

K

L

I

Y

Y

S

D

T

T

I

I

D

D

N

E

S

S

-

N

A

G

F

F

Y

Y

R

V

N

G

D

H

V

A

A

E

A

K

A

G

N

S

R

R

S

S

R

L

M

M

N

N

V

V

P

T

F

F

Q

N

L

L

A

R

D

N

S

E

A

E

L

L

D

N

K

Q

L

Q

F

F

L

L

E

A

E

K

S

A

F

K

A

E

A

Q

G

G

L

F

H

V

A

G

L

L

K

N

G

G

H
|
H

R
|
R

V

S

V

V

G

G

M

C

R
|
R

A

A

S

S

I

I

Y

Y

N

N

A

A

M

V

P

P

L

I

A

D

G

A

V

C

K

I

A

A

L

L

T

R

D

E

F

L

M

M

Q

I

D

Q

F

F

E

K

R

E

active site: W102 (= W102), D172 (= D174), K196 (= K198)
binding pyridoxal-5'-phosphate: A76 (≠ G76), S77 (≠ R77), W102 (= W102), T152 (= T153), D172 (= D174), S174 (= S176), Q195 (= Q197), K196 (= K198), N237 (= N239), T238 (= T240)
binding phosphoserine: H41 (= H41), R42 (= R42), W102 (= W102), T152 (= T153), K196 (= K198), H327 (= H328), R328 (= R329), R334 (= R335)

1w23B Crystal structure of phosphoserine aminotransferase from bacillus alcalophilus (see paper)
42% identity, 99% coverage: 1:359/362 of query aligns to 1:355/357 of 1w23B

query
sites
1w23B

M

V

A

K

Q

Q

V

V

Y

F

N

N

F

F

S

N

S

A

G

G

P

P

A

S

M

A

L

L

P

P

A

K

E

P

V

A

L

L

K

E

L

R

A

A

Q

Q

K

K

E

E

L

L

C

L

D

N

W

F

N

N

G

D

L

T

G

Q

T

M

S

S

V

V

M

M

E

E

I

L

S

S

H

H

R

R

G

S

K

Q

E

S

F

Y

I

E

K

E

V

V

A

H

E

E

E

Q

A

A

E

Q

Q

N

D

L

F

L

R

R

S

E

L

L

N

Q

I

I

P

P

S

N

N

D

Y

Y

K

Q

V

I

L

L

F

F

C

L

H

Q

G

G

G
x
A

R
x
S

G

L

Q

Q

F

F

A

T

G

M

I

L

P

P

L

M

N

N

L

L

G

T

D

K

K

G

T

T

V

I

A

G

D

N

Y

Y

V

V

D

L

A

T

G

G

Y

S

W
|
W

A

S

A

E

S

K

A

A

V

L

K

K

E

E

A

A

H

-

K

K

Y

L

C

L

T

G

P

E

N

T

V

H

I

I

D

A

A

A

K

S

I

T

T

K

V

A

D

N

G

S

L
x
Y

R

Q

A

S

V

I

K

P

P

D

M

F

S

S

E

E

W

F

Q

Q

L

L

T

N

P

E

G

N

A

D

A

A

Y

Y

L

L

H

H

Y

I

C

T

P

S

N

N

E

N

T
|
T

I

I

D
x
Y

G

G

I

T

A

Q

I

Y

E

Q

E

N

A

F

P

P

S

E

F

I

G

N

D

H

D

A

V

P

V

L

V

I

T

-

A

A

D
|
D

L

M

S
|
S

S

S

T

D

I

I

L

L

S

S

R

R

E

P

I

L

D

K

V

V

S

N

R

Q

Y

F

G

G

V

M

I

I

Y

Y

A

A

G

G

A

A

Q
|
Q

K
|
K

N

N

I

L

G

G

P

P

A

S

G

G

L

V

T

T

L

V

I

V

I

I

V

V

R

K

E

K

D

D

L

L

G

V

K

E

A

-

S

-

V

-

A

Q

C

V

P

P

S

T

I

M

L

L

D

Q

Y

Y

S

A

I

T

L

H

S

I

A

K

N

S

D

D

S

S

M

L

F

Y

N
|
N

T
|
T

P

P

T

T

F

F

A

S

W

I

Y

Y

L

M

S

L

G

R

L

N

V

V

F

L

K

D

W

W

L

I

K

K

E

D

Q

L

G

G

V

A

A

E

A

A

M

I

D

A

K

K

I

Q

N

N

Q

E

K

K

A

A

D

K

L

I

Y

Y

S

D

T

T

I

I

D

D

N

E

S

S

-

N

A

G

F

F

Y

Y

R

V

N

G

D
x
H

V
x
A

A

E

A

K

A

G

N

S

R

R

S

S

R

L

M

M

N

N

V

V

P

T

F

F

Q

N

L

L

A

R

D

N

S

E

A

E

L

L

D

N

K

Q

L

Q

F

F

L

L

E

A

E

K

S

A

F

K

A

E

A

Q

G

G

L

F

H

V

A

G

L

L

K

N

G

G

H

H

R

R

V

S

V

V

G

G

M

C

R

R

A

A

S

S

I

I

Y

Y

N

N

A

A

M

V

P

P

L

I

A

D

G

A

V

C

K

I

A

A

L

L

T

R

D

E

F

L

M

M

Q

I

D

Q

F

F

E

K

R

E

active site: W102 (= W102), D172 (= D174), K196 (= K198)
binding magnesium ion: Y127 (≠ L128), Y154 (≠ D155), H285 (≠ D289), A286 (≠ V290)
binding pyridoxal-5'-phosphate: A76 (≠ G76), S77 (≠ R77), W102 (= W102), T152 (= T153), D172 (= D174), S174 (= S176), Q195 (= Q197), K196 (= K198), N234 (= N239), T235 (= T240)

5yb0B Crystal structure of wild type phosphoserine aminotransferase (psat) from e. Histolytica (see paper)
39% identity, 98% coverage: 4:359/362 of query aligns to 1:349/349 of 5yb0B

query
sites
5yb0B

V

I

Y

H

N

N

F

F

S

G

S

A

G

G

P

P

A

A

M

A

L

M

P

A

A

K

E

E

V

V

L

I

K

E

L

A

A

T

Q

A

K

K

E

A

L

V

C

N

D

N

-

F

W

W

N

E

G

G

L

L

G

-

T

-

S

S

V

I

M

L

E

E

I

I

S

S

H

H

R

R

G

S

K

K

E

E

F

W

I

I

K

N

V

V

A

M

E

N

E

E

A

T

E

K

Q

A

D

L

F

M

R

K

S

E

L

V

L

M

N

D

I

I

P

P

S

E

N

G

Y

Y

K

E

V

I

L

L

F

F

C

F

H

G

G

G

G
x
A

R
x
S

G

L

Q

Q

F

F

A

L

G

M

I

V

P

A

L

M

N

N

L

L

G

N

D

K

K

K

T

-

V

-

A

A

D

C

Y

Y

V

L

D

D

A

T

G

G

Y

V

W
|
W

A

A

A

S

S

K

A

A

V

I

K

K

E

E

A

A

H

E

K

-

Y

-

C

-

T

-

P

-

N

N

V

I

I

G

D

E

A

V

K

K

I

I

T

I

-

G

V

T

D

S

G

K

L

D

R

K

A

N

V

Y

K

T

P

Y

M

I

S

P

E

E

W

Y

Q

Q

L

I

T

P

P

S

G

D

A

Y

A

D

Y

Y

L

F

H

H

Y

I

C

T

P

T

N

N

E

N

T
|
T

I

I

D

Y

G

G

I

T

A

E

I

I

E

R

-

K

-

D

-

I

E

E

A

S

P

P

S

-

F

-

G

-

D

-

V

I

V

P

V

L

T

V

A

A

D
|
D

L

M

S
|
S

S

S

T

D

I

I

L

L

S

S

R

K

E

P

I

I

D

D

V

I

S

S

R

K

Y

Y

G

S

V

L

I

I

Y

Y

A

A

G

G

A

A

Q
|
Q

K
|
K

N

N

I

C

G

G

P

A

A

A

G

G

L

V

T

T

L

I

I

V

I

I

V

I

R

K

E

K

D

E

L

I

L

L

G

G

K

K

A

V

S

Q

V

R

A

K

C

I

P

P

S

I

I

I

L

L

D

D

Y

Y

S

Q

I

V

L

H

S

I

A

L

N

N

D

N

S

S

M

M

F

Y

N
|
N

T
|
T

P

P

T

V

F

I

A

S

W

I

Y

F

L

T

S

V

G

N

L

Q

V

T

F

L

K

K

W

Y

L

I

K

K

E

K

Q

I

G

G

V

L

A

K

M

I

D

Q

K

E

I

L

N

N

Q

E

K

K

A

A

D

R

L

L

Y

Y

S

A

T

E

I

I

D

D

N

R

S

N

A

K

F

I

Y

F

R

R

N

G

D

T

V

V

A

R

A

K

N

D

R

R

S

S

R

I

M

M

N

N

V

V

P

C

F

F

Q

V

L

M

A

E

D

E

S

Q

-

Y

-

K

A

Q

L

L

D

E

K

N

L

E

F

F

L

S

E

E

E

Y

S

A

F

L

A

Q

A

K

G

G

L

I

H

I

A

G

L

I

K

K

G

G

H

H

R

R

V

S

V

V

G

G

M

F

R

R

A

A

S

S

I

I

Y

Y

N

N

A

A

M

V

P

T

L

I

A

E

G

S

V

V

K

Q

A

A

L

L

T

I

D

K

F

C

M

M

Q

H

D

D

F

F

E

E

R

Q

binding pyridoxal-5'-phosphate: A72 (≠ G76), S73 (≠ R77), W96 (= W102), T143 (= T153), D162 (= D174), S164 (= S176), Q185 (= Q197), K186 (= K198), N227 (= N239), T228 (= T240)

Query Sequence

>BWI76_RS09755 BWI76_RS09755 phosphoserine transaminase
MAQVYNFSSGPAMLPAEVLKLAQKELCDWNGLGTSVMEISHRGKEFIKVAEEAEQDFRSL
LNIPSNYKVLFCHGGGRGQFAGIPLNLLGDKTVADYVDAGYWAASAVKEAHKYCTPNVID
AKITVDGLRAVKPMSEWQLTPGAAYLHYCPNETIDGIAIEEAPSFGDDVVVTADLSSTIL
SREIDVSRYGVIYAGAQKNIGPAGLTLIIVREDLLGKASVACPSILDYSILSANDSMFNT
PPTFAWYLSGLVFKWLKEQGGVAAMDKINQQKADLLYSTIDNSAFYRNDVAAANRSRMNV
PFQLADSALDKLFLEESFAAGLHALKGHRVVGGMRASIYNAMPLAGVKALTDFMQDFERR
HG

Or try a new SitesBLAST search

SitesBLAST's Database

SitesBLAST's database includes (1) SwissProt entries with experimentally-supported functional features; and (2) protein structures with bound ligands, from the BioLip database.

by Morgan Price, Arkin group
Lawrence Berkeley National Laboratory