SitesBLAST

M1 (= M1) modified: N-formylmethionine; partial
L65 (≠ F65) mutation to V: No change in transport activity.
G96 (≠ S96) mutation to A: No change in transport activity.
A122 (≠ S122) mutation to S: No change in transport activity.
D237 (= D237) mutation D->N,G: Loss of activity.
V264 (= V264) mutation to A: No change in transport activity.
A279 (= A279) mutation to S: No change in transport activity.
C355 (= C355) mutation to Q: No change in transport activity.
K358 (= K358) mutation to T: Loss of activity.
V367 (≠ Y367) mutation to A: Increases transport of melibiose and impairs transport of TMG.

6vbgB Lactose permease complex with thiodigalactoside and nanobody 9043 (see paper)
62% identity, 93% coverage: 1:402/430 of query aligns to 1:390/397 of 6vbgB

query
sites
6vbgB

M

M

F

Y

Y

Y

L

L

K

K

N

N

R

T

N

N

F

F

W

W

I

M

F

F

G

G

A

L

F

F

C

F

F

F

Y

Y

F

F

I

I

M

M

G

G

A

A

V

Y

L
x
F

P

P

F

F

P

P

I

I

W

W

L

L

H

H

D

D

V

I

N

N

Q

H

L

I

D

S

Q

K

R

S

Q

D

T

T

G

W

L

I

V

I

F

F

A

A

S

A

M

I

A

S

L

L

F

F

A

S

L

L

V

L

F

F

Q

Q

P

P

V

L

F

F

G

G

F

L

V

L

T

S

D

D

K

K

F

L

G

G

L

L

K

R

K

K

H

Y

L

L

W

W

M

I

I

I

I

T

F

G

L

M

L

L

L

V

F

M

L

F

A

A

P

P

L

F

F

F

I

I

Y

F

V

I

F

F

S

G

P

P

L

L

Q

Q

S

Y

H

N

F

I

F

L

L

V

G

G

A

S

L

I

L

V

C

G

G

G

I

I

Y

Y

L

L

G

G

L

F

V

C

C

F

S

N

G

A

G

G

S

A

P

P

A

A

I

V

E
|
E

A

A

Y

F

I

I

E

E

K

K

V

V

S

S

R

R

S

S

Q

N

F

F

E

E

Y

F

G

G

R

R

A

A

R
|
R

L

M

F

F

G

G

C
|
C

I

V

G

G

W
|
W

A

A

I

L

C

C

A

A

S

S

I

I

V

V

G

G

Y

I

M

M

F

F

T

T

I

I

N

N

Q

Q

F

F

A

V

F

F

W

W

L

L

A

G

S

S

S

G

F

C

S

A

L

L

V

I

L

L

A

A

G

V

L

L

Y

F

L

F

F

A

K

K

P

T

E

D

E

A

N

-

A

-

T

-

L

-

A

-

V

-

A

-

D

-

G

-

L

-

A

-

L

-

N

N

Q

H

K

S

P

A

I

F

K

S

L

L

K

K

A

L

A

A

L

L

G

E

L

L

L

F

K

R

M

Q

R

P

K

K

F

L

W

W

C

F

L

L

V

S

M

L

Y

Y

I

V

V

I

G

G

V

V

A

S

C

C

V

T

Y

Y

D

D

V

V

F

F

D

D

Q

Q

F

F

A

A

N

N

F

F

T

T

S

S

F

F

R

A

D

T

R

G

H

E

T

Q

G

G

T

T

Q

R

A

V

F

F

G

W

Y

Y

V

V

T

T

P

M

G

G

E
|
E

F

L

L

L

N
|
N

A

A

F

S

I

I

M

M

F

F

A

A

P

P

P

L

I

I

N

N

R

R

I

I

G

G

K

K

N

N

A

A

L

L

I

L

A

A

G

G

M

T

I

I

M

M

S

S

A

V

R

R

I

I

G

G

S

S

L

F

A

A

D

T

S

S

V

A

T

L

E

E

V

V

I

V

I

I

L

L

K

K

T

T

L

L

H
|
H

M

M

F

F

E

E

V

V

P

P

F

F

L

L

L
|
L

V

V

G

G

I

C

F

F

K

K

Y

Y

I

I

T

T

T

S

Q

Q

F

F

N

E

V

V

N

R

L

F

S

S

A

A

S

T

I

I

Y

Y

L

L

W

V

G

C

F

F

C

C

F

F

K

K

Q

Q

L

L

S

A

A

M

I

I

G

F

M

M

S

S

Y

V

A

L

A

A

G

G

M

N

M

M

Y

Y

V

E

T

S

Y

I

G

G

F

F

K

Q

T

G

S

A

Y

Y

L

L

I

V

L

L

G

G

C

L

T

V

A

A

L

L

L

G

F

F

T

T

I

L

I

I

S

S

A

V

F

F

A

T

L

L

S

S

G

G

binding beta-D-galactopyranose: R144 (= R144), C148 (= C148), W151 (= W151), E257 (= E269), N260 (= N272), H310 (= H322)
binding 1-thio-beta-D-galactopyranose: F27 (≠ L27), E126 (= E126), R144 (= R144), L318 (= L330)

2y5yA Crystal structure of lacy in complex with an affinity inactivator (see paper)
61% identity, 93% coverage: 4:402/430 of query aligns to 1:385/386 of 2y5yA

query
sites
2y5yA

L

L

K

K

N

N

R

T

N

N

F

F

W

W

I

M

F

F

G

G

A

L

F

F

C

F

F

F

Y

Y

F
|
F

F

F

I

I

M

M

G

G

A

A

V

Y

L

F

P

P

F

F

P

P

I

I

W

W

L

L

H

H

D

D

V

I

N

N

Q

H

L

I

D

S

Q

K

R

S

Q

D

T

T

G

G

L

I

V

I

F

F

A

A

S

A

M

I

A

S

L

L

F

F

A

S

L

L

V

L

F

F

Q

Q

P

P

V

L

F

F

G

G

F

L

V

L

T

S

D

D

K

K

F

L

G

G

L

L

K

R

K

K

H

Y

L

L

W

W

M

I

I

I

I

T

F

G

L

M

L

L

L

V

F

M

L

F

A

A

P

P

L

F

F

F

I

I

Y

F

V

I

F

F

S

G

P

P

L

L

Q

Q

S

Y

H

N

F

I

F

L

L

V

G

G

A

S

L

I

L

V

C

G

G

G

I

I

Y

Y

L

L

G

G

L

F

V

S

C

F

S

N

G

A

G

G

S
x
C

P

P

A

A

I

V

E

E

A

A

Y

F

I

I

E

E

K

K

V

V

S

S

R

R

S

S

Q

N

F

F

E

E

Y

F

G

G

R

R

A

A

R

R

L

M

F

F

G

G

C

A

I

V

G

G

W
|
W

A

A

I

L

C

V

A

A

S

S

I

I

V

V

G

G

Y

I

M

M

F

F

T

T

I

I

N

N

Q

Q

F

F

A

V

F

F

W

W

L

L

A

G

S

S

S

G

F

M

S

A

L

L

V

I

L

L

A

A

G

V

L

L

Y

F

L

F

F

A

K

K

P

A

E

-

N

-

A

-

T

-

L

-

A

-

V

-

A

-

D

-

G

-

L

-

A

-

L

-

N

N

Q

H

K

S

P

A

I

F

K

S

L

L

K

K

A

L

A

A

L

L

G

E

L

L

L

F

K

R

M

Q

R

P

K

K

F

L

W

W

C

F

L

L

V

S

M

L

Y

Y

I

V

V

I

G

G

V

V

A

S

C

S

V

T

Y

Y

D

D

V

V

F

F

D

D

Q

Q

F

F

A

A

N

N

F

F

T

T

S

S

F

F

R

A

D

T

R

G

H

E

T

Q

G

G

T

T

Q

R

A

V

F

F

G

G

Y

Y

V

V

T

T

P

M

G

G

E
|
E

F

L

L

L

N

N

A

A

F

S

I

I

M

M

F

F

A

A

P

P

P

L

I

I

N

N

R

R

I

I

G

G

K

K

N

N

A

A

L

L

I

L

A

A

G

G

M

T

I

I

M

M

S

S

A

V

R

R

I

I

G

G

S

S

L

F

A

A

D

T

S

S

V

A

T

L

E

E

V

V

I

V

I

I

L

L

K

K

T

T

L

L

H

H

M

M

F

F

E

E

V

V

P

P

F

F

L

L

V

V

G

G

I

S

F

F

K

K

Y

Y

I

I

T

T

T

S

Q

Q

F

F

N

E

V

V

N

R

L

F

S

S

A

A

S

T

I

I

Y

Y

L

L

W

V

G

A

F

F

C

A

F

F

K

K

Q

Q

L

L

S

A

A

M

I

I

G

F

M

M

S

S

Y

V

A

L

A

A

G

G

M

N

M

M

Y

Y

V

E

T

S

Y

I

G

G

F

F

K

Q

T

G

S

A

Y

Y

L

L

I

V

L

L

G

G

C

L

T

V

A

A

L

L

L

G

F

F

T

T

I

L

I

I

S

S

A

V

F

F

A

T

L

L

S

S

G

G

binding 2-sulfanylethyl beta-D-galactopyranoside: F17 (= F20), C119 (≠ S122), W148 (= W151), E252 (= E269)

4zyrA Crystal structure of e. Coli lactose permease g46w/g262w bound to p- nitrophenyl alpha-d-galactopyranoside (alpha-npg) (see paper)
61% identity, 89% coverage: 22:402/430 of query aligns to 17:381/387 of 4zyrA

query
sites
4zyrA

I

I

M
|
M

G

G

A

A

V

Y

L

F

P

P

F

F

P

P

I

I

W

W

L

L

H

H

D

D

V

I

N

N

Q

H

L

I

D

S

Q

K

R

S

Q

D

T

T

G

W

L

I

V

I

F

F

A

A

S

A

M

I

A

S

L

L

F

F

A

S

L

L

V

L

F

F

Q

Q

P

P

V

L

F

F

G

G

F

L

V

L

T

S

D

D

K

K

F

L

G

G

L

L

K

R

K

K

H

Y

L

L

W

W

M

I

I

I

I

T

F

G

L

M

L

L

L

V

F

M

L

F

A

A

P

P

L

F

F

F

I

I

Y

F

V

I

F

F

S

G

P

P

L

L

Q

Q

S

Y

H

N

F

I

F

L

L

V

G

G

A

S

L

I

L

V

C

G

G

G

I

I

Y

Y

L

L

G

G

L

F

V

C

C

F

S

N

G

A

G

G

S

A

P

P

A

A

I

V

E

E

A

A

Y

F

I

I

E

E

K

K

V

V

S

S

R

R

S

S

Q

N

F

F

E

E

Y

F

G

G

R

R

A

A

R
|
R

L

M

F

F

G

G

C
|
C

I

V

G

G

W
|
W

A

A

I

L

C

C

A

A

S

S

I

I

V

V

G

G

Y

I

M

M

F

F

T

T

I

I

N

N

Q

Q

F

F

A

V

F

F

W

W

L

L

A

G

S

S

S

G

F

C

S

A

L

L

V

I

L

L

A

A

G

V

L

L

Y

F

L

F

F

A

K

K

P

T

E

D

E

A

N

-

A

-

T

-

L

-

A

-

V

-

A

-

D

-

G

-

L

-

A

-

L

-

N

-

Q

-

K

-

P

-

I

F

K

S

L

L

K

K

A

L

A

A

L

L

G

E

L

L

L

F

K

R

M

Q

R

P

K

K

F

L

W

W

C

F

L

L

V

S

M

L

Y

Y

I

V

V

I

G

G

V

V

A

S

C

C

V

T

Y

Y

D

D

V

V

F

F

D

D

Q

Q

F

F

A

A

N

N

F

F

T

T

S

S

F

F

R

A

D

T

R

G

H

E

T

Q

G

G

T

T

Q

R

A

V

F

F

G

W

Y

Y

V

V

T

T

P

M

G

G

E
|
E

F

L

L

L

N

N

A

A

F

S

I

I

M

M

F

F

A

A

P

P

P

L

I

I

N

N

R

R

I

I

G

G

K

K

N

N

A

A

L

L

I

L

A

A

G

G

M

T

I

I

M

M

S

S

A

V

R

R

I

I

G

G

S

S

L

F

A

A

D

T

S

S

V

A

T

L

E

E

V

V

I

V

I

I

L

L

K

K

T

T

L

L

H
|
H

M

M

F

F

E

E

V

V

P

P

F

F

L

L

V

V

G

G

I

C

F

F

K

K

Y

Y

I

I

T

T

T

S

Q

Q

F

F

N

E

V

V

N

R

L

F

S

S

A

A

S

T

I

I

Y

Y

L

L

W

V

G

C

F

F

C

C

F

F

K

K

Q

Q

L

L

S

A

A

M

I

I

G

F

M

M

S

S

Y

V

A

L

A

A

G

G

M

N

M

M

Y

Y

V

E

T

S

Y

I

G

G

F

F

K

Q

T

G

S

A

Y

Y

L

L

I

V

L

L

G

G

C

L

T

V

A

A

L

L

L

G

F

F

T

T

I

L

I

I

S

S

A

V

F

F

A

T

L

L

S

S

G

G

binding 4-nitrophenyl alpha-D-galactopyranoside: M18 (= M23), R139 (= R144), C143 (= C148), W146 (= W151), E248 (= E269), H301 (= H322)

P16552 Raffinose permease; Raf permease from Escherichia coli (see paper)
53% identity, 93% coverage: 5:403/430 of query aligns to 8:406/425 of P16552

query
sites
P16552

K

K

N

N

R

T

N

D

F

F

W

W

I

I

F

F

G

G

A

L

F

F

C

F

F

F

F

L

Y

Y

F

F

I

I

M

M

G

A

A

T

V

C

L

F

P

P

F

F

F

L

P

P

I
x
V

W

W

L

L

H

S

D

D

V

V

N

V

Q

G

L

L

D

S

Q

K

R

T

Q

D

T

T

G

G

L

I

V

V

F

F

A

S

S

C

M

L

A

S

L

L

F

F

A

A

L

I

V

S

F

F

Q

Q

P

P

V

L

F

L

G

G

F

V

V

I

T

S

D

D

K

R

F

L

G

G

L

L

K

K

H

N

L

L

L

I

W

W

M

S

I

I

I

S

F

L

L

L

L

V

F

F

L

F

A

A

P

P

L

F

F

F

I

L

Y

Y

V

V

F

F

S

A

P

P

L

L

Q

H

S

L

H

N

F

I

F

W

L

A

G

G

A

A

L

L

L

T

C

G

G

G

I

V

Y

F

L

I

G

G

L

F

V

V

C

F

S

S

G

A

G

G

S

A

P

G

A

A

I

I

E

E

A

A

Y

Y

I

I

E

E

K

R

V

V

S

S

R

R

R
x
S

S
|
S

Q

G

F

F

E

E

Y

Y

G

G

R

K

A

A

R

R

L

M

F

F

G

G

C

C

I

L

G

G

W

W

A

A

I

L

C

C

A

A

S

T

I

M

V

A

G

G

Y

I

M

L

F

F

T

N

I

V

N

D

N

P

Q

S

F

L

A

V

F

F

W

W

L

M

A

G

S

S

S

G

F

G

S

A

L

L

V

L

L

L

A

L

G

L

L

L

Y

Y

L

L

F

A

K

R

P

P

E

S

E

T

N

S

A

Q

T

T

L

A

A

M

V

V

A

M

D

N

G

A

L

L

A

G

L

A

N

N

Q

S

K

S

P

L

I

I

K

S

L

T

K

R

A

M

A

V

L

F

G

S

L

L

L

F

K

R

M

M

R

R

K

Q

F

M

W

W

C

M

L

F

V

V

M

L

Y

Y

I

T

V

I

G

G

V

V

A

A

C

C

V

V

Y

Y

D

D

V

V

F

F

D

D

Q

Q

F

F

A

A

N

I

F

F

T

R

S

S

F

F

R

D

D

T

R

P

H

Q

T

A

G

G

T

I

Q

K

A

A

F

F

G

G

Y

F

V

A

T

T

P

A

G

G

E

E

F

I

L

C

N

N

A

A

F

I

I

I

M

M

F

F

F

C

A

T

P

P

P

W

I

I

N

N

R

R

I

I

G

G

G

A

K

K

N

N

A

T

L

L

I

V

A

A

G

G

M

G

I

I

M

M

S

T

A

I

R

R

I

I

I

T

G

G

S

S

S

A

L

F

A

A

D

T

S

T

V

M

T

T

E

E

V

V

I

V

I

I

L

L

K

K

T

M

L

L

H

H

M

A

F

L

E

E

V

V

P

P

F

F

L

L

V

V

G

G

I

A

F

F

K

K

Y

Y

I

I

T

T

T

G

Q

V

F

F

N

D

V

T

N

R

L

L

S

S

A

A

S

T

I

V

Y

Y

L

L

W

I

G

G

F

F

C

Q

F

F

F

S

K

K

Q

Q

L

L

S

A

A

A

I

I

G

L

M

L

S

S

Y

T

A

F

A

A

G

G

M

H

M

L

Y

Y

V

D

T

R

Y

M

G

G

F

F

K

Q

T

N

S

T

Y

Y

L

F

I

V

L

L

G
|
G

C

M

T
x
I

A

V

L

L

L

T

F

V

T

T

I

V

I

I

S

S

A

A

F

F

A

T

L

L

S

S

G

S

K

S

V35 (≠ I32) mutation to A: Significantly reduces raffinose transport, enhances maltose transport.
S138 (≠ R135) mutation to D: Significantly reduces raffinose transport, enhances maltose transport; when associated with L-139 and A-389.
S139 (= S136) mutation to L: Significantly reduces raffinose transport, enhances maltose transport; when associated with D-138 and A-389.
G389 (= G386) mutation to A: Significantly reduces raffinose transport, enhances maltose transport; when associated with D-138 and L-139.
I391 (≠ T388) mutation to S: Significantly reduces raffinose transport, enhances maltose transport.

P96517 Melibiose permease; Melibiose transporter MelY from Enterobacter cloacae subsp. cloacae (strain ATCC 13047 / DSM 30054 / NBRC 13535 / NCTC 10005 / WDCM 00083 / NCDC 279-56) (see 2 papers)
56% identity, 93% coverage: 3:403/430 of query aligns to 8:408/425 of P96517

query
sites
P96517

Y

H

L

K

K

D

N

N

R

P

N

N

F

F

W

W

I

I

F

F

G

G

A

L

F

F

C

F

F

F

F

L

Y

Y

F

F

I

I

M

M

G

A

A

T

V

C

L

F

P

P

F

F

F

L

P

P

I

I

W

W

L

L

H

S

D

D

V

I

N

I

Q

G

L

L

D

N

Q

K

R

T

Q

H

T

T

G

G

L

I

V

V

F

F

A

S

S

C

M

I

A

S

L

L

F

S

A

A

L

I

V

A

F

F

Q

Q

P

P

V

V

F

L

G

G

F

V

V

I

T

S

D

D

K

K

F

L

G

G

L

L

K

K

H

H

L

L

W

W

M

I

I

I

I

S

F

V

L
|
L

L

L

L

F

F
x
L

L

F

A

A

P

P

L

F

F

F

I

L

Y

Y

V

V

F

F

S

A

P

P

L

L

Q

K

S

T

H

N

F

I

F

W

L

L

G

G

A

A

L

L

L

S

C

G

G

G

I

L

Y

Y

L

I

G

G

L

F

V

V

C

F

S

S

G

A

G

G

S

S

P

G

A

A

I

I

E

E

A

A

Y

Y

I

I

E

E

K

R

V

V

S

S

R

R

R

N

S

S

Q

A

F

F

E

E

Y

Y

G

G

R

K

A

A

R

R

L

M

F

F

G

G

C

C

I

L

G

G

W

W

A

G

I

L

C

C

A

A

S

S

I

T

V

G

G

G

Y

I

M

L

F

F

T

G

I

I

N

D

N

P

Q

S

F

Y

A

V

F

F

W

W

L

M

A

G

S

S

S

A

F

A

S
x
A

L

L

V

L

L

L

A

M

G

L

L

L

Y

V

L

V

F

A

K

K

P

P

E

K

E

P

N

N

A

Q

T

T

L

A

A

Q

V

V

A

M

D

N

G

A

L

L

A

G

L

A

N

N

Q

Q

K

P

P

Q

I

I

K

T

L

A

K

K

A

K

A

V

L

F

G

N

L

L

L

F

K

R

M

Q

R

R

K

R

F

M

W

W

C

M

L

F

V

I

M

L

Y

Y

I

V

V

I

G

G

V

V

A

A

C

C

V

V

Y

Y

D

D

V

V

F

F

D

D

Q

Q

F

F

A

A

N

T

F

F

T

K

S

T

F

F

R

A

D

T

R

P

H

Q

T

E

G

G

T

T

Q

R

A

A

F

F

G

G

Y

F

V

A

T

T

P

A

G

G

E

E

F

I

L

C

N

N

A

A

F

I

I

I

M

M

F

F

F

C

A

S

P

P

P

W

I

I

N

N

R

R

I

I

G

G

G

A

K

K

N

N

A

T

L

L

I

I

A

A

G

G

M

L

I

I

M

M

S

A

A

T

R

R

I

I

G

G

S

S

L

F

A

A

D

T

S

T

V

A

T

V

E

E

V

V

I

I

I

A

L

L

K

K

T

M

L

L

H

H

M

A

F

L

E

E

V

V

P

P

F

F

L

L

V

V

G

G

I

A

F

F

K

K

Y

Y

I

I

T

T

T

G

Q

V

F

F

N

D

V

T

N

R

L

L

S

S

A

A

S

T

I

I

Y

Y

L

L

W

I

G

G

F

F

C

Q

F

F

F

A

K

K

Q

Q

L

S

S

A

A
|
A

I

I

G

F

M

L

S

S

Y

A

A

F

A

A

G

G

M

N

M

M

Y

Y

V

D

T

R

Y

I

G

G

F

F

K

Q

T

E

S

T

Y

Y

L

L

I

M

L

L

G

G

C

C

T

F

A

V

L

L

L

A

F

I

T

T

I

V

S

S

A

A

F

F

A

T

L

L

S

S

G

S

K

R

L88 (= L83) mutation to P: Does not affect melibiose transport. Increases affinity for maltose and maltose transport.
L91 (≠ F86) mutation to P: Does not affect melibiose transport. Increases affinity for maltose and maltose transport.
A182 (≠ S177) mutation to P: Does not affect melibiose transport. Increases affinity for maltose and maltose transport.
A367 (= A362) mutation to V: No change in transport activity.

Query Sequence

>BWI76_RS10315 FitnessBrowser__Koxy:BWI76_RS10315
MFYLKNRNFWIFGAFCFFYFFIMGAVLPFFPIWLHDVNQLDQRQTGLVFASMALFALVFQ
PVFGFVTDKFGLKKHLLWMIIFLLLFLAPLFIYVFSPLLQSHFFLGALLCGIYLGLVCSG
GSPAIEAYIEKVSRRSQFEYGRARLFGCIGWAICASIVGYMFTINNQFAFWLASSFSLVL
AGLLYLFKPEENATLAVADGLALNQKPIKLKAALGLLKMRKFWCLVMYIVGVACVYDVFD
QQFANFFTSFFRDRHTGTQAFGYVTTPGEFLNAFIMFFAPPIINRIGGKNALLIAGMIMS
ARIIGSSLADSVTEVIILKTLHMFEVPFLLVGIFKYITTQFNVNLSASIYLWGFCFFKQL
SAIGMSYAAGMMYVTYGFKTSYLILGCTALLFTIISAFALSGKVRRATGQPAQDVSLSNI
KGEFGSEAQR

Or try a new SitesBLAST search

SitesBLAST's Database

SitesBLAST's database includes (1) SwissProt entries with experimentally-supported functional features; and (2) protein structures with bound ligands, from the BioLip database.

by Morgan Price, Arkin group
Lawrence Berkeley National Laboratory