SitesBLAST

W45 (= W11) Important for C5-methylcytosine-recognition; mutation to A: Abolished binding to C5-methylcytosine (m5C)-containing mRNAs.; mutation to F: Decreased binding to C5-methylcytosine (m5C)-containing mRNAs. Decreased ability to discriminate between m5C-containing and unmethylated mRNAs.
WFNVRN 45:50 (≠ WFNESK 11:16) C5-methylcytosine binding

P92186 Protein lin-28; Abnormal cell lineage protein 28 from Caenorhabditis elegans (see 3 papers)
44% identity, 79% coverage: 7:61/70 of query aligns to 55:109/227 of P92186

query
sites
P92186

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G55 (= G7) mutation to S: In n1119; loss of function.
M87 (≠ S39) mutation to I: In ga73; when associated with Q-91.
R91 (≠ K43) mutation to Q: In ga73; when associated with I-87.

Sites not aligning to the query:

2:32 mutation Missing: Constitutively active and results in reduced lin-28 protein levels. Rescues the seam cell number and vulval developmental defects of the lin-28 n719 loss of function mutant.
11 D→A: Reduced ced-3-mediated cleavage in vitro.
23 E→A: Reduced ced-3-mediated cleavage in vitro.
24 D→A: Reduced ced-3-mediated cleavage in vitro.
31 D→A: Loss of ced-3-mediated cleavage in vitro. Partially prevents reduction of lin-28 protein levels in vivo. About 20 percent of mutants fail to reach adulthood. Defects in alae morphology.
31:32 Cleavage; by ced-3
32 N→M: Loss of ced-3-mediated cleavage in vitro. Prevents reduction of lin-28 protein levels in vitro.
128 G→E: In ma157.; G→R: In sy283.
133 P→S: In ve9.

P67809 Y-box-binding protein 1; YB-1; CCAAT-binding transcription factor I subunit A; CBF-A; DNA-binding protein B; DBPB; Enhancer factor I subunit A; EFI-A; Nuclease-sensitive element-binding protein 1; Y-box transcription factor from Homo sapiens (Human) (see 5 papers)
39% identity, 96% coverage: 1:67/70 of query aligns to 55:125/324 of P67809

query
sites
P67809

M
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W65 (= W11) Important for C5-methylcytosine-recognition; mutation to A: Abolished binding to C5-methylcytosine (m5C)-containing mRNAs.; mutation to F: Decreased binding to C5-methylcytosine (m5C)-containing mRNAs. Decreased ability to discriminate between m5C-containing and unmethylated mRNAs.
WFNVRN 65:70 (≠ WFNESK 11:16) C5-methylcytosine binding
S102 (≠ T44) modified: Phosphoserine; by PKB/AKT1; mutation to A: Loss of phosphorylation by PKB/AKT1. Inhibits translocation to the nucleus and tumor cell growth.

Sites not aligning to the query:

1 modified: Initiator methionine, Removed
2 modified: N-acetylserine
15:71 Interaction with ss-DNA
137 modified: Glycyl lysine isopeptide (Lys-Gly) (interchain with G-Cter in ubiquitin)
165 modified: Phosphoserine
301 modified: N6-acetyllysine; K→A: Abrogates unconventional secretion.
304 modified: N6-acetyllysine; K→A: Abrogates unconventional secretion.

Q28618 Y-box-binding protein 1; YB-1; Nuclease-sensitive element-binding protein 1; Y-box transcription factor; p50 from Oryctolagus cuniculus (Rabbit) (see 2 papers)
39% identity, 96% coverage: 1:67/70 of query aligns to 55:125/324 of Q28618

query
sites
Q28618

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S102 (≠ T44) modified: Phosphoserine; by PKB/AKT1; mutation to A: Abolishes phosphorylation by PKB/AKT1.

Sites not aligning to the query:

219:220 Cleavage; by 20S proteasomal protease

Q9Z2C8 Y-box-binding protein 2; FRGY2 homolog; Germ cell-specific Y-box-binding protein from Mus musculus (Mouse) (see paper)
40% identity, 91% coverage: 4:67/70 of query aligns to 95:162/360 of Q9Z2C8

query
sites
Q9Z2C8

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Y109 (≠ F18) mutation to A: Abolishes cytoplasmic retention and mRNA-binding affinity; when associated with A-111.
F111 (= F20) mutation to A: Abolishes cytoplasmic retention and mRNA-binding affinity; when associated with A-109.

Sites not aligning to the query:

89:171 Required for cytoplasmic retention
219:360 Required for mRNA-binding

Q6ZN17 Protein lin-28 homolog B; Lin-28B from Homo sapiens (Human) (see paper)
46% identity, 80% coverage: 6:61/70 of query aligns to 31:93/250 of Q6ZN17

query
sites
Q6ZN17

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Sites not aligning to the query:

112:125 Bipartite nuclear localization signal
239:250 Nucleolar localization signal

4alpA The lin28b cold shock domain in complex with hexauridine (see paper)
44% identity, 80% coverage: 6:61/70 of query aligns to 8:70/87 of 4alpA

query
sites
4alpA

T

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binding : G19 (= G17), Q43 (≠ F34), F51 (= F42), R52 (≠ K43)

4a75A The lin28b cold shock domain in complex with hexathymidine. (see paper)
44% identity, 80% coverage: 6:61/70 of query aligns to 10:72/89 of 4a75A

query
sites
4a75A

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binding : K14 (= K10), W15 (= W11), N17 (= N13), F22 (= F18), F24 (= F20), D40 (= D29), F42 (= F31), H44 (= H33), S69 (≠ G58), K71 (= K60)

Sites not aligning to the query:

binding : 73

5udzA Human lin28a in complex with let-7f-1 microrna pre-element (see paper)
41% identity, 80% coverage: 6:61/70 of query aligns to 9:71/139 of 5udzA

query
sites
5udzA

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binding : W14 (= W11), F15 (= F12), N16 (= N13), V17 (≠ E14), R18 (≠ S15), M19 (≠ K16), F21 (= F18), F23 (= F20), F41 (= F31), H43 (= H33), Q44 (≠ F34), F52 (= F42), R53 (≠ K43), K70 (= K60), G71 (= G61)

Sites not aligning to the query:

binding zinc ion: 98, 101, 106, 111, 120, 123, 128, 133
binding : 73, 93, 95, 97, 98, 99, 107, 108, 116, 118, 119, 120, 121, 122, 129, 130, 136

Query Sequence

>BWI76_RS10395
MSNKMTGLVKWFNESKGFGFITPDNGSKDVFVHFSAIQSGGFKTLNEGQKVEFTIEDGQK
GPSAGNVYAI

Or try a new SitesBLAST search

SitesBLAST's Database

SitesBLAST's database includes (1) SwissProt entries with experimentally-supported functional features; and (2) protein structures with bound ligands, from the BioLip database.

by Morgan Price, Arkin group
Lawrence Berkeley National Laboratory