SitesBLAST

SitesBLAST – Find functional sites

SitesBLAST

Comparing BWI76_RS10650 FitnessBrowser__Koxy:BWI76_RS10650 to proteins with known functional sites using BLASTp with E ≤ 0.001.

Or try Sites on a Tree, PaperBLAST, Conserved Domains, or compare to all protein structures

Found 2 hits to proteins with known functional sites (download)

P20966 PTS system fructose-specific EIIB'BC component; EIIB'BC-Fru; EC 2.7.1.202 from Escherichia coli (strain K12) (see paper)
44% identity, 80% coverage: 5:94/113 of query aligns to 106:194/563 of P20966

query
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P20966

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C112 (= C11) active site, Phosphocysteine intermediate; for EIIB activity

O31645 PTS system mannose-specific EIIBCA component; EIIBCA-Man; EII-Man; EC 2.7.1.191 from Bacillus subtilis (strain 168) (see 2 papers)
37% identity, 82% coverage: 5:97/113 of query aligns to 3:94/650 of O31645

query
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O31645

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C9 (= C11) mutation to A: Appears to be unable to inactivate ManR, since ManR does not show any decrease in its DNA-binding activity in the presence of this mutant.

Sites not aligning to the query:

365 modified: Phosphoserine

Query Sequence

>BWI76_RS10650 FitnessBrowser__Koxy:BWI76_RS10650
MDFTIVAVTACVSGVAHTYMAAERLEKVGHQEKWNIKIETQGALGVENKITAEDIAHVDV
VLLVTDIEIDDAQRFHGIRTIKTSIKTFLLQPQQIVEAVKCIMKKPVVYIEIP

Or try a new SitesBLAST search

SitesBLAST's Database

SitesBLAST's database includes (1) SwissProt entries with experimentally-supported functional features; and (2) protein structures with bound ligands, from the BioLip database.

by Morgan Price, Arkin group
Lawrence Berkeley National Laboratory