SitesBLAST

SitesBLAST – Find functional sites

SitesBLAST

Comparing BWI76_RS10665 FitnessBrowser__Koxy:BWI76_RS10665 to proteins with known functional sites using BLASTp with E ≤ 0.001.

Or try Sites on a Tree, PaperBLAST, Conserved Domains, or compare to all protein structures

Found 2 hits to proteins with known functional sites (download)

P20966 PTS system fructose-specific EIIB'BC component; EIIB'BC-Fru; EC 2.7.1.202 from Escherichia coli (strain K12) (see paper)
53% identity, 83% coverage: 3:90/106 of query aligns to 105:191/563 of P20966

query
sites
P20966

K

R

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A

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C
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G

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D

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C112 (= C10) active site, Phosphocysteine intermediate; for EIIB activity

O31645 PTS system mannose-specific EIIBCA component; EIIBCA-Man; EII-Man; EC 2.7.1.191 from Bacillus subtilis (strain 168) (see 2 papers)
45% identity, 90% coverage: 3:97/106 of query aligns to 2:95/650 of O31645

query
sites
O31645

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K

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C9 (= C10) mutation to A: Appears to be unable to inactivate ManR, since ManR does not show any decrease in its DNA-binding activity in the presence of this mutant.

Sites not aligning to the query:

365 modified: Phosphoserine

Query Sequence

>BWI76_RS10665 FitnessBrowser__Koxy:BWI76_RS10665
MTKIIAVTACPSGVAHTYMAAESLESAAKAKGWQVKVETQGSIGIENELTAEDVASADLV
ILTKDIGIKFEERFAGKTIVRVNISDAVKRAEAIMNKIDSHLSQNA

Or try a new SitesBLAST search

SitesBLAST's Database

SitesBLAST's database includes (1) SwissProt entries with experimentally-supported functional features; and (2) protein structures with bound ligands, from the BioLip database.

by Morgan Price, Arkin group
Lawrence Berkeley National Laboratory