SitesBLAST

A0A1J1DL12 2-iminobutanoate/2-iminopropanoate deaminase; Allergen Der f 34; Enamine/imine deaminase; Allergen Der f 34.0101; EC 3.5.99.10 from Dermatophagoides farinae (American house dust mite) (see paper)
30% identity, 96% coverage: 2:126/130 of query aligns to 3:127/128 of A0A1J1DL12

query
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A0A1J1DL12

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Y19 (≠ F18) mutation to A: Loss of imine deaminase activity; when associated with A-33; A-59; A-91; A-105; A-114 and A-120.
G33 (= G32) mutation to A: Loss of imine deaminase activity; when associated with A-19; A-59; A-91; A-105; A-114 and A-120.
N59 (≠ T58) mutation to A: Loss of imine deaminase activity; when associated with A-19; A-33; A-91; A-105; A-114 and A-120.
N91 (= N90) mutation to A: Loss of imine deaminase activity; when associated with A-19; A-33; A-59; A-105; A-114 and A-120.
R105 (= R104) mutation to A: Loss of imine deaminase activity; when associated with A-19; A-33; A-59; A-91; A-114 and A-120.
P114 (≠ K113) mutation to A: Loss of imine deaminase activity; when associated with A-19; A-33; A-59; A-91; A-105 and A-120.
E120 (= E119) mutation to A: Loss of imine deaminase activity; when associated with A-19; A-33; A-59; A-91; A-105 and A-114.

Sites not aligning to the query:

1 modified: Initiator methionine, Removed

2uynA Crystal structure of e. Coli tdcf with bound 2-ketobutyrate (see paper)
35% identity, 85% coverage: 15:124/130 of query aligns to 13:124/127 of 2uynA

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2uynA

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binding 2-ketobutyric acid: F83 (≠ Y86), R104 (= R104), S105 (≠ F105), C106 (= C106)

2uykC Crystal structure of e. Coli tdcf with bound serine (see paper)
35% identity, 85% coverage: 15:124/130 of query aligns to 13:124/127 of 2uykC

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2uykC

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binding serine: R104 (= R104), S105 (≠ F105), C106 (= C106)

Q7CP78 2-iminobutanoate/2-iminopropanoate deaminase; Enamine/imine deaminase; EC 3.5.99.10 from Salmonella typhimurium (strain LT2 / SGSC1412 / ATCC 700720) (see paper)
32% identity, 85% coverage: 15:124/130 of query aligns to 14:125/128 of Q7CP78

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Q7CP78

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Y17 (≠ F18) mutation to F: Deaminase activity is defective. At pH 8, strongly reduces the increase in rate of 2-ketobutyrate formation over the rate of IlvA alone. At pH 9.5, moderately reduces the increase in rate of 2-ketobutyrate formation over the rate of IlvA alone.
R105 (= R104) mutation to A: Deaminase activity is defective. At pH 8 and 9.5, strongly reduces the increase in rate of 2-ketobutyrate formation over the rate of IlvA alone.
E120 (= E119) Important for catalytic activity at high pH; mutation to A: Deaminase activity is defective. At pH 8, moderately reduces the increase in rate of 2-ketobutyrate formation over the rate of IlvA alone. At pH 9.5, strongly reduces the increase in rate of 2-ketobutyrate formation over the rate of IlvA alone.; mutation to K: Deaminase activity is defective. At pH 8, moderately reduces the increase in rate of 2-ketobutyrate formation over the rate of IlvA alone. At pH 9.5, strongly reduces the increase in rate of 2-ketobutyrate formation over the rate of IlvA alone.

2b33B Crystal structure of a putative endoribonuclease (tm0215) from thermotoga maritima msb8 at 2.30 a resolution
28% identity, 85% coverage: 15:124/130 of query aligns to 13:122/126 of 2b33B

query
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2b33B

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binding calcium ion: D35 (= D37), E37 (≠ Q39)

3k0tC Crystal structure of pspto -psp protein in complex with d-beta-glucose from pseudomonas syringae pv. Tomato str. Dc3000 (see paper)
33% identity, 91% coverage: 3:120/130 of query aligns to 1:117/124 of 3k0tC

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3k0tC

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binding beta-D-glucopyranose: Y16 (≠ F18), G30 (= G32), I32 (≠ L34), F83 (≠ Y86), R101 (= R104), R111 (≠ P114), E116 (= E119)

3vczB 1.80 angstrom resolution crystal structure of a putative translation initiation inhibitor from vibrio vulnificus cmcp6
30% identity, 85% coverage: 15:124/130 of query aligns to 13:125/127 of 3vczB

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3vczB

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binding magnesium ion: D79 (= D82), N81 (≠ K84)

Q94JQ4 Reactive Intermediate Deaminase A, chloroplastic; 2-iminobutanoate/2-iminopropanoate deaminase; EC 3.5.99.10 from Arabidopsis thaliana (Mouse-ear cress) (see paper)
24% identity, 95% coverage: 3:126/130 of query aligns to 64:187/187 of Q94JQ4

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R165 (= R104) mutation to A: Loss of activity.

P80601 2-iminobutanoate/2-iminopropanoate deaminase; 14.3 kDa perchloric acid soluble protein; Translation inhibitor L-PSP ribonuclease; UK114 antigen; EC 3.5.99.10; EC 3.1.-.- from Capra hircus (Goat) (see paper)
24% identity, 98% coverage: 1:127/130 of query aligns to 4:130/137 of P80601

query
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P80601

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Sites not aligning to the query:

1 modified: Initiator methionine, Removed
2 modified: N-acetylserine

P52759 2-iminobutanoate/2-iminopropanoate deaminase; Liver perchloric acid-soluble protein; L-PSP; Reactive intermediate imine deaminase A homolog; Translation inhibitor L-PSP ribonuclease; UK114 antigen homolog; rp14.5; EC 3.5.99.10 from Rattus norvegicus (Rat) (see paper)
23% identity, 96% coverage: 3:127/130 of query aligns to 6:130/137 of P52759

query
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P52759

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Sites not aligning to the query:

1 modified: Initiator methionine, Removed
2 modified: N-acetylserine

5hp8A Crystal structures of rida in complex with pyruvate (see paper)
26% identity, 79% coverage: 24:126/130 of query aligns to 6:108/108 of 5hp8A

query
sites
5hp8A

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binding pyruvic acid: R86 (= R104), T88 (≠ C106)

3i3fA Hypothetical protein from giardia lamblia gl50803_14299
28% identity, 78% coverage: 23:123/130 of query aligns to 24:126/131 of 3i3fA

query
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3i3fA

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binding pentanoic acid: G33 (= G32), R108 (= R104), C109 (≠ F105), C110 (= C106)

Sites not aligning to the query:

binding pentanoic acid: 16, 19

3i3fB Hypothetical protein from giardia lamblia gl50803_14299
28% identity, 78% coverage: 23:123/130 of query aligns to 21:123/128 of 3i3fB

query
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3i3fB

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binding butanoic acid: R105 (= R104), C106 (≠ F105), C107 (= C106)
binding pentanoic acid: Q113 (≠ K113)

Sites not aligning to the query:

binding pentanoic acid: 13, 16

3lybB Structure of putative endoribonuclease(kp1_3112) from klebsiella pneumoniae
30% identity, 62% coverage: 50:129/130 of query aligns to 58:137/137 of 3lybB

query
sites
3lybB

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Sites not aligning to the query:

binding calcium ion: 28, 32

Query Sequence

>BWI76_RS10770 FitnessBrowser__Koxy:BWI76_RS10770
MPKQVIIPPGTTTPIAPFVPGTLADGVVYVSGTLPFDKQNNVVYIGDPKAQTRHVLETIK
SVIETAGGSMEDVTFNSIFITDWKNYAAINEVYAEFFPGDKPARFCIQCGLVKPDALVEI
ATVAHIGKPA

Or try a new SitesBLAST search

SitesBLAST's Database

SitesBLAST's database includes (1) SwissProt entries with experimentally-supported functional features; and (2) protein structures with bound ligands, from the BioLip database.

by Morgan Price, Arkin group
Lawrence Berkeley National Laboratory