SitesBLAST

SitesBLAST – Find functional sites

SitesBLAST

Comparing BWI76_RS11130 FitnessBrowser__Koxy:BWI76_RS11130 to proteins with known functional sites using BLASTp with E ≤ 0.001.

Or try Sites on a Tree, PaperBLAST, Conserved Domains, or compare to all protein structures

Found 4 hits to proteins with known functional sites (download)

P69786 PTS system glucose-specific EIICB component; EIICB-Glc; EII-Glc; EC 2.7.1.199 from Escherichia coli (strain K12) (see 4 papers)
97% identity, 100% coverage: 1:477/477 of query aligns to 1:477/477 of P69786

query
sites
P69786

M

M

F

F

K

K

N

N

A

A

F

F

A

A

N

N

L

L

Q

Q

K

K

V

V

G

G

K

K

S

S

L

L

M

M

L

L

P

P

V

V

S

S

V

V

L

L

P

P

I

I

A

A

G

G

I

I

L

L

L

L

G

G

V

V

G

G

S

S

A

A

N

N

F

F

S

S

W

W

L

L

P

P

A

A

V

V

V

V

S

S

H

H

V

V

M

M

A

A

E

E

A

A

G

G

G

G

S

S

V

V

F

F

A

A

N

N

M

M

P

P

L

L

I

I

F

F

A

A

I

I

G

G

V

V

A

A

L

L

G

G

F

F

T

T

N

N

N

N

D

D

G

G

V

V

S

S

A

A

L

L

A

A

S

A

V

V

V

V

A

A

Y

Y

G

G

I

I

M

M

V

V

K

K

T

T

M

M

S

A

V

V

V

V

A

A

P

P

L

L

V

V

L

L

H

H

L

L

P

P

A

A

E

E

E

E

I

I

A

A

A

S

K

K

H

H

L

L

A

A

D

D

T

T

G

G

V

V

L

L

G

G

G

G

I

I

I

I

S

S

G

G

A

A

I

I

A

A

A

A

Y

Y

M

M

F

F

N

N

R

R

F

F

Y

Y

R

R

I

I

K

K

L

L

P

P

E

E

Y

Y

L

L

G

G

F

F

F

F

A

A

G

G

K

K

R

R

F

F

V

V

P

P

I

I

I

I

S

S

G

G

L

L

A

A

A

A

I

I

F

F

T

T

G

G

V

V

I

V

L

L

S

S

F

F

I

I

W

W

P

P

P

P

I

I

G

G

T

S

A

A

I

I

Q

Q

T

T

F

F

S

S

Q

Q

W

W

A

A

A

A

Y

Y

Q

Q

N

N

P

P

V

V

V

V

A

A

F

F

G

G

I

I

Y

Y

G

G

F

F

I

I

E

E

R

R

C
|
C

L

L

V

V

P

P

F

F

G

G

L

L

H

H

H

H

I

I

W

W

N

N

V

V

P

P

F

F

Q

Q

M

M

Q

Q

I

I

G

G

E

E

Y

Y

T

T

N

N

A

A

A

A

G

G

Q

Q

V

V

F

F

H

H

G

G

D

D

I

I

P

P

R

R

Y

Y

M

M

A

A

G

G

D

D

P

P

T

T

A

A

G

G

K

K

L

L

S

S

G

G

G

G

F

F

L

L

F

F

K

K

M

M

Y

Y

G

G

L

L

P

P

A

A

A

A

A

A

I

I

A

A

I

I

W

W

H

H

S

S

A

A

K

K

P

P

E

E

N

N

R

R

A

A

K

K

V

V

G

G

G

G

I

I

M

M

I

I

S

S

A

A

A

A

L

L

T

T

S

S

F

F

L

L

T

T

G

G

I

I

T

T

E

E

P

P

I

I

E

E

F

F

S

S

F

F

M

M

F

F

V

V

A

A

P

P

I

I

L

L

Y

Y

I

I

I

I

H

H

A

A

I

I

L

L

A

A

G

G

L

L

A

A

F

F

P

P

I

I

C
|
C

I

I

L

L

L

L

G

G

M

M

R

R

D

D

G

G

T

T

S

S

F

F

S

S

H

H

G

G

L

L

I

I

D

D

F

F

I

I

V

V

L

L

S

S

G

G

N

N

S

S

S

S

K

K

L

L

W

W

L

L

F

F

P

P

I

I

V

V

G

G

I

I

C

G

Y

Y

A

A

I

I

V

V

Y

Y

Y

Y

V

T

V

I

F

F

R

R

V

V

L

L

I

I

K

K

A

A

L

L

D

D

L

L

K

K

T

T

P

P

G

G

R

R

E

E

D

D

A

A

T

T

E

E

D

D

S

A

K

K

A

A

G

T

A

G

T

T

S

S

E

E

M

M

A

A

P

P

A

A

L

L

I

V

A

A

A

A

F

F

G

G

G

G

K

K

E

E

N

N

I

I

T

T

N

N

L

L

D

D

A

A

C
|
C

I

I

T

T

R

R

L

L

R

R

V

V

S

S

V

V

A

A

D

D

V

V

A

S

K

K

V

V

D

D

Q

Q

A

A

G

G

L

L

K

K

K

K

L

L

G

G

A

A

A

A

G

G

V

V

V
|
V

V

V

A
|
A

G

G

S

S

G

G

V

V

Q
|
Q

A

A

I

I

F

F

G

G

T

T

K

K

S

S

D

D

N

N

L

L

K

K

T

T

E

E

M

M

D

D

E

E

Y

Y

I

I

R

R

S

N

N

H

C204 (= C204) mutation to S: Destabilizes the protein structure; when associated with S-326.
C326 (= C326) mutation to S: Destabilizes the protein structure; when associated with S-204.
C421 (= C421) modified: Phosphocysteine; mutation to S: Unable to be phosphorylated and to catalyze the phosphoryl exchange between glucose and glucose 6-phosphate at equilibrium. Remains susceptible to CdiA toxin from E.coli strain 3006.
V449 (= V449) mutation to Q: Interaction with Mlc is hardly detectable.
A451 (= A451) mutation to F: The complex with mlc shows much weaker association than the wild-type.
Q456 (= Q456) mutation to A: Interaction with Mlc is hardly detectable.

P09323 PTS system N-acetylglucosamine-specific EIICBA component; EIICBA-Nag; EII-Nag; EC 2.7.1.193 from Escherichia coli (strain K12) (see paper)
41% identity, 99% coverage: 4:474/477 of query aligns to 2:466/648 of P09323

query
sites
P09323

N

N

A

I

F

L

A

G

N

F

L

F

Q

Q

K

R

V

L

G

G

K

R

S

A

L

L

M

Q

L

L

P

P

V

I

S

A

V

V

L

L

P

P

I

V

A

A

G

A

I

L

L

L

L

L

G

R

V

F

G

G

S

Q

A

P

N

D

F

L

S

-

W

-

L

-

P

-

A

-

V

L

V

N

S

V

H

A

V

F

M

I

A

A

E

Q

A

A

G

G

G

G

S

A

V

I

F

F

A

D

N

N

M

L

P

A

L

L

I

I

F

F

A

A

I

I

G

G

V

V

A

A

L

S

G

S

F

W

T

S

N

K

N

D

D

S

G

A

V

G

S

A

A

A

L

A

A

L

S

A

V

G

V

A

A

V

-

G

Y

Y

G

F

I

V

M

L

V

T

K

K

T

A

M

M

S

V

V

T

V

I

A

N

P

P

L

E

V

I

L

-

H

-

L

-

P

-

A

-

E

-

E

-

I

-

A

-

A

-

K

-

H

-

L

-

A

-

D

N

T

M

G

G

V

V

L

L

G

A

G

G

I

I

I

I

S

T

G

G

A

L

I

V

A

G

A

G

Y

A

M

A

F

Y

N

N

R

R

F

W

Y

S

R

D

I

I

K

K

L

L

P

P

E

D

Y

F

L

L

G

S

F

F

F

F

A

G

G

G

K

K

R

R

F

F

V

V

P

P

I

I

I

A

S

T

G

G

L

F

A

F

A

C

I

L

F

V

T

L

G

A

V

A

I

I

L

F

S

G

F

Y

I

V

W

W

P

P

P

P

I

V

G

Q

T

H

A

A

I

I

Q

H

T

A

F

G

S

G

Q

E

W

W

A

I

A

V

Y

S

Q

A

N

G

P

-

V

A

V

L

A

G

F

S

G

G

I

I

Y

F

G

G

F

F

I

I

E

N

R

R

C

L

L

L

V

I

P

P

F

T

G

G

L

L

H

H

H

Q

I

V

W

L

N

N

V

T

P

I

F

A

Q

W

M

F

Q

Q

I

I

G

G

E

E

Y

F

T

T

N

N

A

A

A

A

G

G

Q

T

V

V

F

F

H

H

G

G

D

D

I

I

P

N

R

R

Y

F

M

Y

A

A

G

G

D

D

P

G

T

T

A

A

G

G

K

M

-

F

L

M

S

S

G

G

G

F

F

F

L

P

F

I

K

M

M

M

Y

F

G

G

L

L

P

P

A

G

A

A

A

A

I

L

A

A

I

M

W

Y

H

F

S

A

A

A

K

P

P

K

E

E

N

R

R

R

A

P

K

M

V

V

G

G

G

G

I

M

M

L

I

L

S

S

A

V

A

A

L

V

T

T

S

A

F

F

L

L

T

T

G

G

I

V

T

T

E

E

P

P

I

L

E

E

F

F

S

L

F

F

M

M

F

F

V

L

A

A

P

P

I

L

L

L

Y

Y

I

L

I

L

H

H

A

A

I

L

L

L

A

T

G

G

L

I

A

S

F

L

P

F

I

V

C

A

I

T

L

L

L

L

G

G

M

I

R

H

D

A

G

G

T

F

S

S

F

F

S

S

H

A

G

G

L

A

I

I

D

D

F

Y

I

A

V

L

-

M

-

Y

-

N

L

L

S

P

G

A

N

A

S

S

S

Q

K

N

L

V

W

W

L

M

F

L

P

L

I

V

V

M

G

G

I

V

C

I

Y

F

A

F

I

A

V

I

Y

Y

Y

F

V

V

V

V

F

F

R

S

V

L

L

V

I

I

K

R

A

M

L

F

D

N

L

L

K

K

T

T

P

P

G

G

R

R

E

E

D

D

-

K

-

E

-

D

-

E

-

I

A

V

T

T

E

E

D

E

S

A

K

N

A

S

G

N

A

T

T

E

-

E

-

G

-

L

S

T

E

Q

M

L

A

A

P

T

A

N

L

Y

I

I

A

A

A

A

F

V

G

G

G

G

K

T

E

D

N

N

I

L

T

K

N

A

L

I

D

D

A

A

C

C

I

I

T

T

R

R

L

L

R

R

V

L

S

T

V

V

A

A

D

D

V

S

A

A

K

R

V

V

D

N

Q

D

A

T

G

M

L

C

K

K

K

R

L

L

G

G

A

A

A

S

G

G

V

V

V

V

-

K

V

L

A

N

G

K

S

Q

G

T

V

I

Q

Q

A

V

I

I

F

V

G

G

T

A

K

K

S

A

D

E

N

S

L

I

K

G

T

D

E

A

M

M

D

K

E

K

Y

V

I

V

Sites not aligning to the query:

1 modified: N-formylmethionine

6bvgA Crystal structure of bcmalt t280c-e54c crosslinked by divalent mercury (see paper)
31% identity, 80% coverage: 5:386/477 of query aligns to 1:442/447 of 6bvgA

query
sites
6bvgA

A

S

F

F

A

D

N

F

L

W

Q

Q

K

K

V

F

G

G

K

K

S

A

L

L

M

L

L

V

P

V

V
|
V

S

A

V

V

L

M

P

P

I

A

A

A

G

G

I

L

L

M

L

I

G

S

V

I

G

G

-

K

-

L

-

I

-

G

-

M

S

S

A

A

N

G

F

D

S

I

W

N

L

A

P

V

A

H

V

T

V

I

S

A

H

R

V

V

M

M

A

C

E

D

A

I

G

G

G

W

S

A

V

I

F

I

A

T

N

N

M

L

P

H

L

I

I

L

F

F

A

A

I

V

G

A

V

I

A

G

L

G

G

S

F

W

T

A

N

K

N

D

-

R

-

A

-

G

-

G

-

A

-

F

-

A

-

A

-

L

-

L

-

A

-

F

-

V

-

L

-

T

-

N

-

R

-

I

-

T

-

G

-

A

-

I

-

F

-

G

-

V

-

N

-

A

-

E

-

M

-

L

-

A

D

D

G

S

V

K

S

A

A

K

L

V

A

S

S

S

V

V

V

L

A

A

Y

G

G

D

I

L

M

I

V

V

K

K

T

D

M

Y

S

F

V

T

V

S

A

-

P

-

L

-

V

V

L

L

H

G

L

A

P

P

A

A

E

-

E

-

I

-

A

-

A

-

K

-

H

-

L

-

A

L

D

N

T

M

G

G

V

V

L

F

G

V

G

G

I

I

I

I

S

T

G

G

A

F

I

L

A

G

A

A

Y

T

M

L

F

Y

N

N

R

K

F

Y

Y

Y

R

N

I

Y

-

N

K

K

L

L

P

P

E

Q

Y

A

L

L

G

A

F

F

F

F

A

N

G

G

K

K

R

R

F

F

V

V

P

P

I

F

I

V

S

V

G

I

L

V

A

W

A

S

I

T

F

V

T

T

G

A

V

I

I

V

L

L

S

S

F

L

I

L

W

W

P

P

P

F

I

I

G

Q

T

S

A

G

I

L

Q

N

T

E

F

F

S

G

Q

R

W

W

A

I

A

A

Y

A

Q

S

N

K

-

D

-

S

-

A

P

P

V

I

V

V

A

A

F

P

G

F

I

V

Y

Y

G

G

F

T

I

L

E
|
E

R
|
R

C

L

L

L

V
x
L

P

P

F

F

G

G

L

L

H
|
H

H
|
H

I

M

W

L

N
x
T

V

I

P

P

F

M

Q
x
N

M
x
Y

Q

T

I

E

-

L

-

G

G

G

E

T

Y

Y

T

T

-

M

-

L

-

T

-

G

N

S

A

K

A

V

G

G

Q

Q

V

V

F

V

H

A

G

G

D

Q

I

D

P

P

R

L

Y

W

M

L

A

A

-

W

-

I

-

C

-

D

-

L

-

N

-

N

-

L

-

L

-

A

-

N

G

G

D

D

P

T

T

K

A

A

G

Y

K

N

-

D

-

L

-

L

-

N

-

N

-

V

-

V

-

P

-

A

-

R

-

F

L
x
K

S

A

G

G

G
x
Q

F

V

L

I

F

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K

S

M

T

Y

A

G

A

L

L

P

M

A

G

A

I

A

A

I

F

A

A

I

M

W

F

H

R

S

N

A

V

K

D

P

K

E

E

N

K

R

R

A

A

K

K

V

Y

G

K

G

P

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M

F

I

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S

A

A

A

A

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L

T

A

S

V

F

F

L

L

T

T

G

G

I
x
V

T
|
T

E
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E

P

P

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E

F

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M

F

F

M

M

F

F

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A

A

P

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I

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Y

Y

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H

Y

A

A

I

I

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T

A

T

G

G

L

L

A

A

F

F

P

A

I

L

C

A

I

D

L

L

L

I

G

N

M

L

R

R

D

-

G

-

T

-

S

V

F

H

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A

H
x
F

G

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I

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I

-

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R

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Y

F

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binding alpha-D-glucopyranose: V16 (= V20), E227 (= E202), R228 (= R203), L231 (≠ V206), H236 (= H211), H237 (= H212), H237 (= H212), T240 (≠ N215), N244 (≠ Q219), Y245 (≠ M220), K303 (≠ L250), Q306 (≠ G253), Q306 (≠ G253), V349 (≠ I296), T350 (= T297), E351 (= E298), F389 (≠ H339)

1o2fB Complex of enzyme iiaglc and iibglc phosphocarrier protein hpr from escherichia coli nmr, restrained regularized mean structure (see paper)
95% identity, 16% coverage: 400:476/477 of query aligns to 1:77/77 of 1o2fB

query
sites
1o2fB

E

E

M

M

A

A

P

A

A

A

L

L

I

V

A

A

A

A

F

F

G

G

G

G

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K

E

E

N

N

I

I

T

T

N

N

L

L

D

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A

A

C
|
C

I
|
I

T
|
T

R
|
R

L

L

R

R

V

V

S

S

V

V

A

A

D

D

V

V

A

S

K

K

V

V

D

D

Q

Q

A

A

G

G

L

L

K

K

K

K

L

L

G

G

A

A

A

A

G

G

V

V

V

V

V

V

A

A

G

G

S

S

G

G

V

V

Q

Q

A

A

I

I

F

F

G

G

T

T

K

K

S

S

D

D

N

N

L

L

K

K

T

T

E

E

M

M

D

D

E

E

Y

Y

I

I

R

R

S

N

binding phosphite ion: C22 (= C421), I23 (= I422), T24 (= T423), R25 (= R424)

Query Sequence

>BWI76_RS11130 FitnessBrowser__Koxy:BWI76_RS11130
MFKNAFANLQKVGKSLMLPVSVLPIAGILLGVGSANFSWLPAVVSHVMAEAGGSVFANMP
LIFAIGVALGFTNNDGVSALASVVAYGIMVKTMSVVAPLVLHLPAEEIAAKHLADTGVLG
GIISGAIAAYMFNRFYRIKLPEYLGFFAGKRFVPIISGLAAIFTGVILSFIWPPIGTAIQ
TFSQWAAYQNPVVAFGIYGFIERCLVPFGLHHIWNVPFQMQIGEYTNAAGQVFHGDIPRY
MAGDPTAGKLSGGFLFKMYGLPAAAIAIWHSAKPENRAKVGGIMISAALTSFLTGITEPI
EFSFMFVAPILYIIHAILAGLAFPICILLGMRDGTSFSHGLIDFIVLSGNSSKLWLFPIV
GICYAIVYYVVFRVLIKALDLKTPGREDATEDSKAGATSEMAPALIAAFGGKENITNLDA
CITRLRVSVADVAKVDQAGLKKLGAAGVVVAGSGVQAIFGTKSDNLKTEMDEYIRSN

Or try a new SitesBLAST search

SitesBLAST's Database

SitesBLAST's database includes (1) SwissProt entries with experimentally-supported functional features; and (2) protein structures with bound ligands, from the BioLip database.

by Morgan Price, Arkin group
Lawrence Berkeley National Laboratory