SitesBLAST
Comparing BWI76_RS11775 FitnessBrowser__Koxy:BWI76_RS11775 to proteins with known functional sites using BLASTp with E ≤ 0.001.
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Found 20 (the maximum) hits to proteins with known functional sites (download)
4za2D Crystal structure of pectobacterium carotovorum 2-keto-3-deoxy-d- gluconate dehydrogenase complexed with NAD+ (see paper)
74% identity, 100% coverage: 1:253/253 of query aligns to 1:247/247 of 4za2D
- binding nicotinamide-adenine-dinucleotide: G17 (= G17), D19 (= D19), L22 (= L22), I42 (≠ R42), D65 (= D65), M66 (≠ L66), N92 (= N92), A93 (= A93), G94 (= G94), L115 (= L115), I143 (= I143), S145 (= S145), Y158 (= Y158), K162 (= K162), G189 (= G189), M191 (= M191), T193 (= T193), N195 (= N195)
4hp8B Crystal structure of a putative 2-deoxy-d-gluconate 3-dehydrogenase from agrobacterium tumefaciens (target efi-506435) with bound NADP
57% identity, 99% coverage: 4:253/253 of query aligns to 2:246/246 of 4hp8B
- active site: G19 (= G21), S138 (= S145), V148 (= V155), Y151 (= Y158), K155 (= K162)
- binding nadp nicotinamide-adenine-dinucleotide phosphate: G15 (= G17), N17 (≠ D19), T18 (= T20), G19 (= G21), L20 (= L22), R40 (= R42), R41 (= R43), D63 (= D65), F64 (≠ L66), N85 (= N92), G87 (= G94), I88 (≠ T95), I136 (= I143), Y151 (= Y158), K155 (= K162), P181 (= P188), G182 (= G189), I184 (≠ M191), T186 (= T193), N188 (= N195), T189 (= T196)
3uf0A Crystal structure of a putative NAD(p) dependent gluconate 5- dehydrogenase from beutenbergia cavernae(efi target efi-502044) with bound NADP (low occupancy)
47% identity, 98% coverage: 6:252/253 of query aligns to 3:248/249 of 3uf0A
- active site: G18 (= G21), S141 (= S145), V151 (= V155), Y154 (= Y158), K158 (= K162)
- binding nadp nicotinamide-adenine-dinucleotide phosphate: G14 (= G17), S17 (≠ T20), G18 (= G21), I19 (≠ L22), R39 (= R42), D63 (= D65), L64 (= L66), N89 (= N92), G91 (= G94), I92 (≠ T95), I139 (= I143), A140 (= A144), S141 (= S145), Y154 (= Y158), K158 (= K162), P184 (= P188), G185 (= G189), V187 (≠ M191), T189 (= T193), N191 (= N195), T192 (= T196)
4jroC Crystal structure of 3-oxoacyl-[acyl-carrier protein]reductase (fabg) from listeria monocytogenes in complex with NADP+
41% identity, 96% coverage: 8:251/253 of query aligns to 3:246/247 of 4jroC
- active site: G16 (= G21), S142 (= S145), Q152 (≠ V155), Y155 (= Y158), K159 (= K162)
- binding nadp nicotinamide-adenine-dinucleotide phosphate: G12 (= G17), S14 (≠ D19), R15 (≠ T20), G16 (= G21), I17 (≠ L22), N35 (≠ V40), Y36 (≠ N41), N37 (≠ R42), G38 (≠ R43), S39 (≠ I44), N63 (≠ D65), V64 (≠ L66), N90 (= N92), A91 (= A93), I93 (≠ T95), I113 (≠ L115), S142 (= S145), Y155 (= Y158), K159 (= K162), P185 (= P188), I188 (≠ M191), T190 (= T193)
1vl8B Crystal structure of gluconate 5-dehydrogenase (tm0441) from thermotoga maritima at 2.07 a resolution
37% identity, 98% coverage: 6:252/253 of query aligns to 2:251/252 of 1vl8B
- active site: G17 (= G21), S143 (= S145), I154 (≠ V155), Y157 (= Y158), K161 (= K162)
- binding nadp nicotinamide-adenine-dinucleotide phosphate: G13 (= G17), R16 (≠ T20), G17 (= G21), L18 (= L22), S37 (≠ N41), R38 (= R42), C63 (≠ A64), D64 (= D65), V65 (≠ L66), A91 (≠ N92), A92 (= A93), G93 (= G94), I94 (≠ T95), V114 (≠ L115), I141 (= I143), S143 (= S145), Y157 (= Y158), K161 (= K162), P187 (= P188), G188 (= G189), Y190 (≠ M191), T192 (= T193), M194 (≠ N195), T195 (= T196)
5u9pB Crystal structure of a gluconate 5-dehydrogenase from burkholderia cenocepacia j2315 in complex with NADP and tartrate
42% identity, 98% coverage: 3:249/253 of query aligns to 9:256/261 of 5u9pB
- active site: G27 (= G21), S152 (= S145), Y165 (= Y158), K169 (= K162)
- binding nadp nicotinamide-adenine-dinucleotide phosphate: G23 (= G17), R26 (≠ T20), G27 (= G21), I28 (≠ L22), R48 (= R43), D73 (= D65), V74 (≠ L66), N100 (= N92), A101 (= A93), I150 (= I143), Y165 (= Y158), K169 (= K162), P195 (= P188), F198 (≠ M191), T200 (= T193), L202 (≠ N195), N203 (≠ T196)
3o03A Quaternary complex structure of gluconate 5-dehydrogenase from streptococcus suis type 2 (see paper)
38% identity, 99% coverage: 3:252/253 of query aligns to 4:248/254 of 3o03A
- active site: G22 (= G21), S147 (= S145), V157 (= V155), Y160 (= Y158), K164 (= K162)
- binding calcium ion: S147 (= S145), M148 (= M146), P190 (= P188)
- binding D-gluconic acid: I99 (= I96), R101 (= R98), S147 (= S145), M149 (≠ L147), R154 (≠ G152), Y160 (= Y158)
- binding nadp nicotinamide-adenine-dinucleotide phosphate: G18 (= G17), Y21 (≠ T20), G22 (= G21), I23 (≠ L22), D42 (≠ G39), I43 (≠ V40), L47 (≠ I44), D68 (= D65), V69 (≠ L66), N95 (= N92), A96 (= A93), G97 (= G94), I145 (= I143), Y160 (= Y158), K164 (= K162), P190 (= P188)
1edoA The x-ray structure of beta-keto acyl carrier protein reductase from brassica napus complexed with NADP+ (see paper)
37% identity, 94% coverage: 12:249/253 of query aligns to 3:241/244 of 1edoA
- active site: G12 (= G21), S138 (= S145), Y151 (= Y158), K155 (= K162)
- binding nadp nicotinamide-adenine-dinucleotide phosphate: G8 (= G17), S10 (≠ D19), R11 (≠ T20), I13 (≠ L22), N31 (= N41), Y32 (≠ R42), A33 (vs. gap), R34 (vs. gap), S35 (vs. gap), D59 (= D65), V60 (≠ L66), N86 (= N92), A87 (= A93), S138 (= S145), Y151 (= Y158), K155 (= K162), P181 (= P188), G182 (= G189), I184 (≠ M191), S186 (≠ T193), M188 (≠ N195)
3osuA Crystal structure of the 3-oxoacyl-acyl carrier protein reductase, fabg, from staphylococcus aureus
38% identity, 95% coverage: 11:250/253 of query aligns to 5:244/246 of 3osuA
3sj7A Structure of beta-ketoacetyl-coa reductase (fabg) from staphylococcus aureus complex with NADPH (see paper)
37% identity, 95% coverage: 11:250/253 of query aligns to 2:237/239 of 3sj7A
- active site: G12 (= G21), S138 (= S145), Q148 (≠ V155), Y151 (= Y158), K155 (= K162)
- binding nadph dihydro-nicotinamide-adenine-dinucleotide phosphate: G8 (= G17), S10 (≠ D19), R11 (≠ T20), I13 (≠ L22), N31 (= N41), Y32 (≠ R42), A33 (≠ R43), G34 (≠ I44), S35 (≠ P45), A58 (= A64), N59 (≠ D65), V60 (≠ L66), N86 (= N92), A87 (= A93), T109 (≠ L115), S138 (= S145), Y151 (= Y158), K155 (= K162), P181 (= P188), G182 (= G189)
3op4A Crystal structure of putative 3-ketoacyl-(acyl-carrier-protein) reductase from vibrio cholerae o1 biovar eltor str. N16961 in complex with NADP+ (see paper)
36% identity, 97% coverage: 6:250/253 of query aligns to 4:244/247 of 3op4A
- binding nadp nicotinamide-adenine-dinucleotide phosphate: G15 (= G17), S17 (≠ D19), R18 (≠ T20), I20 (≠ L22), T40 (≠ I44), N62 (≠ D65), V63 (≠ L66), N89 (= N92), A90 (= A93), I92 (≠ T95), V139 (≠ I143), S141 (= S145), Y154 (= Y158), K158 (= K162), P184 (= P188), G185 (= G189), I187 (≠ M191), T189 (= T193), M191 (≠ N195)
P73574 3-oxoacyl-[acyl-carrier-protein] reductase; 3-ketoacyl-acyl carrier protein reductase; EC 1.1.1.100 from Synechocystis sp. (strain PCC 6803 / Kazusa) (see paper)
37% identity, 96% coverage: 8:251/253 of query aligns to 4:245/247 of P73574
- A14 (≠ C18) mutation to G: 4.2-fold increase in activity on acetoacetyl-CoA.
- P151 (≠ I153) mutation to F: 2.7-fold increase in activity on acetoacetyl-CoA.; mutation to V: 5.7-fold increase in activity on acetoacetyl-CoA.
- K160 (= K162) mutation to A: Almost no activity on acetoacetyl-CoA.
- F188 (≠ Y190) mutation to Y: 3.3-fold increase in activity on acetoacetyl-CoA.
- N198 (≠ R205) mutation to R: 3.5-fold increase in activity on acetoacetyl-CoA.
P0AEK2 3-oxoacyl-[acyl-carrier-protein] reductase FabG; 3-ketoacyl-acyl carrier protein reductase; Beta-Ketoacyl-acyl carrier protein reductase; Beta-ketoacyl-ACP reductase; EC 1.1.1.100 from Escherichia coli (strain K12) (see 2 papers)
35% identity, 97% coverage: 6:250/253 of query aligns to 1:241/244 of P0AEK2
- GASR 12:15 (≠ GCDT 17:20) binding
- T37 (≠ I44) binding
- NV 59:60 (≠ DL 65:66) binding
- N86 (= N92) binding
- Y151 (= Y158) mutation to F: Defect in the affinity for NADPH.
- YAAAK 151:155 (≠ YTASK 158:162) binding
- A154 (≠ S161) mutation to T: Decreases in the thermolability of the reductase; when associated with K-233.
- K155 (= K162) mutation to A: Defect in the affinity for NADPH.
- I184 (≠ M191) binding
- E233 (≠ Y242) mutation to K: Decreases in the thermolability of the reductase; when associated with T-154.
6oz7A Putative oxidoreductase from escherichia coli str. K-12
37% identity, 95% coverage: 11:251/253 of query aligns to 2:233/242 of 6oz7A
1q7bA The structure of betaketoacyl-[acp] reductase from e. Coli in complex with NADP+ (see paper)
35% identity, 96% coverage: 7:250/253 of query aligns to 1:240/243 of 1q7bA
- active site: G15 (= G21), E101 (≠ D108), S137 (= S145), Q147 (≠ V155), Y150 (= Y158), K154 (= K162)
- binding calcium ion: E232 (≠ Y242), T233 (= T243)
- binding nadp nicotinamide-adenine-dinucleotide phosphate: G11 (= G17), S13 (≠ D19), R14 (≠ T20), T36 (≠ I44), N58 (≠ D65), V59 (≠ L66), N85 (= N92), A86 (= A93), G87 (= G94), I88 (≠ T95), S137 (= S145), Y150 (= Y158), K154 (= K162), P180 (= P188), G181 (= G189), I183 (≠ M191)
2ztlA Closed conformation of d-3-hydroxybutyrate dehydrogenase complexed with NAD+ and l-3-hydroxybutyrate (see paper)
39% identity, 97% coverage: 8:253/253 of query aligns to 2:260/260 of 2ztlA
- active site: G15 (= G21), N114 (= N116), S142 (= S145), Y155 (= Y158), K159 (= K162), L200 (≠ D202)
- binding (3s)-3-hydroxybutanoic acid: Q94 (≠ I96), S142 (= S145), H144 (≠ L147), K152 (≠ V155), Y155 (= Y158), Q196 (= Q198)
- binding nicotinamide-adenine-dinucleotide: G11 (= G17), G15 (= G21), I16 (≠ L22), F36 (≠ R43), L64 (= L66), N90 (= N92), A91 (= A93), G92 (= G94), L113 (= L115), Y155 (= Y158), K159 (= K162), P185 (= P188), W187 (≠ Y190), V188 (≠ M191), T190 (= T193), V193 (≠ T196)
1wmbA Crystal structure of NAD dependent d-3-hydroxybutylate dehydrogenase (see paper)
39% identity, 97% coverage: 8:253/253 of query aligns to 2:260/260 of 1wmbA
7tzpG Crystal structure of putataive short-chain dehydrogenase/reductase (fabg) from klebsiella pneumoniae subsp. Pneumoniae ntuh-k2044 in complex with nadh (see paper)
37% identity, 98% coverage: 4:251/253 of query aligns to 2:246/247 of 7tzpG
- binding 1,4-dihydronicotinamide adenine dinucleotide: G15 (= G17), R18 (≠ T20), G19 (= G21), I20 (≠ L22), D39 (≠ N41), R40 (= R42), C63 (≠ A64), I65 (≠ L66), N91 (= N92), G93 (= G94), I94 (≠ T95), V114 (≠ L115), Y155 (= Y158), K159 (= K162), I188 (≠ M191), T190 (= T193), T193 (= T196)
4i08A Crystal structure of beta-ketoacyl-acyl carrier protein reductase (fabg) from vibrio cholerae in complex with NADPH (see paper)
35% identity, 97% coverage: 6:250/253 of query aligns to 4:240/243 of 4i08A
- active site: G19 (= G21), N113 (= N116), S141 (= S145), Q151 (≠ V155), Y154 (= Y158), K158 (= K162)
- binding nadph dihydro-nicotinamide-adenine-dinucleotide phosphate: G15 (= G17), S17 (≠ D19), R18 (≠ T20), I20 (≠ L22), T40 (≠ I44), N62 (≠ D65), V63 (≠ L66), N89 (= N92), A90 (= A93), G140 (≠ A144), S141 (= S145), Y154 (= Y158), K158 (= K162), P184 (= P188), G185 (= G189), T189 (= T193)
1q7cA The structure of betaketoacyl-[acp] reductase y151f mutant in complex with NADPH fragment (see paper)
35% identity, 96% coverage: 7:250/253 of query aligns to 1:240/243 of 1q7cA
- active site: G15 (= G21), S137 (= S145), Q147 (≠ V155), F150 (≠ Y158), K154 (= K162)
- binding nadph dihydro-nicotinamide-adenine-dinucleotide phosphate: G11 (= G17), S13 (≠ D19), R14 (≠ T20), A35 (≠ R43), T36 (≠ I44), L57 (≠ A64), N58 (≠ D65), V59 (≠ L66), G87 (= G94), I88 (≠ T95)
Query Sequence
>BWI76_RS11775 FitnessBrowser__Koxy:BWI76_RS11775
MVLNAFDLAGKVAIVTGCDTGLGQGMTLGLAQAGCDIVGVNRRIPHETAEKVQALGRRFT
AIQADLSRQDEIEDIVTQAVAAMGRIDILVNNAGTIRRADALSFSEKDWDDVMNLNLKSV
FFLSQAVARQFIQQGDGGKIVNIASMLSFQGGIRVPSYTASKSGVLGITRLMANEWAGHR
INVNAIAPGYMATNNTQQLRDDAERSKAILDRIPAGRWGVADDLQGPVVFLASRAADYIS
GYTLAVDGGWLAR
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SitesBLAST's Database
SitesBLAST's database includes
(1) SwissProt
entries with experimentally-supported functional features;
and (2) protein structures with bound ligands, from the
BioLip database.
by Morgan Price,
Arkin group
Lawrence Berkeley National Laboratory