SitesBLAST

SitesBLAST – Find functional sites

SitesBLAST

Comparing BWI76_RS11855 FitnessBrowser__Koxy:BWI76_RS11855 to proteins with known functional sites using BLASTp with E ≤ 0.001.

Or try Sites on a Tree, PaperBLAST, Conserved Domains, or compare to all protein structures

Found 20 (the maximum) hits to proteins with known functional sites (download)

1kgzB Crystal structure analysis of the anthranilate phosphoribosyltransferase from erwinia carotovora (current name, pectobacterium carotovorum) (see paper)
73% identity, 63% coverage: 199:530/531 of query aligns to 1:330/330 of 1kgzB

query
sites
1kgzB

T

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binding manganese (ii) ion: S92 (= S290), D222 (= D422), E223 (= E423), E223 (= E423)
binding 1-O-pyrophosphono-5-O-phosphono-alpha-D-ribofuranose: V79 (= V277), G82 (= G280), G83 (= G281), N90 (= N288), I91 (= I289), S92 (= S290), T93 (= T291), K108 (= K306), S118 (= S318)

1i7qB Anthranilate synthase from s. Marcescens (see paper)
81% identity, 36% coverage: 2:193/531 of query aligns to 1:192/192 of 1i7qB

query
sites
1i7qB

A

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active site: G58 (= G59), C83 (= C84), L84 (= L85), H169 (= H170), E171 (= E172)
binding glutamic acid: P55 (= P56), G56 (= G57), G58 (= G59), C83 (= C84), L84 (= L85), Q87 (= Q88), H132 (= H133), S133 (= S134), L134 (= L135)

P00900 Anthranilate synthase component 2; AS; ASII; Anthranilate synthase, GATase component; Anthranilate synthase, glutamine amidotransferase component; EC 4.1.3.27 from Serratia marcescens (see 3 papers)
81% identity, 36% coverage: 1:193/531 of query aligns to 1:193/193 of P00900

query
sites
P00900

M
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M1 (= M1) modified: Initiator methionine, Removed
C84 (= C84) active site, Nucleophile; for GATase activity

5nofA Anthranilate phosphoribosyltransferase from thermococcus kodakaraensis (see paper)
38% identity, 60% coverage: 203:518/531 of query aligns to 3:311/325 of 5nofA

query
sites
5nofA

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x
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binding zinc ion: E48 (≠ Q250), D78 (= D282), E198 (= E403), D217 (= D422), E218 (= E423)

4yi7A Anthranilate bound at active site of anthranilate phosphoribosyl transferase from acinetobacter (anprt; trpd)
41% identity, 48% coverage: 200:452/531 of query aligns to 4:243/331 of 4yi7A

query
sites
4yi7A

L

I

Q

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L

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G

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N

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L

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A

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I

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E

V

I

S

S

L

L

H

A

A

S

P

Q

T

T

V

Y

V

I

A

A

E

E

L

L

N

K

N

N

G

G

E

E

I

V

K

T

S

E

Y

W

Q

V

L

L

T

N

A

P

A

E

D

D

F

V

G

N

L

I

active site: V82 (= V277)
binding 2-aminobenzoic acid: V82 (= V277), G83 (= G278), H110 (= H307), R154 (= R364), G167 (= G377)

1zxyA Anthranilate phosphoribosyltransferase from sulfolobus solfataricus in complex with prpp and magnesium (see paper)
35% identity, 54% coverage: 199:485/531 of query aligns to 2:286/344 of 1zxyA

query
sites
1zxyA

T

N

L

I

Q

N

P

E

I

I

L

L

E

K

K

K

L

L

Y

I

Q

N

A

K

E

S

T

D

L

L

S

E

Q

I

Q

N

E

E

S

A

H

E

Q

E

L

L

F

A

S

K

A

A

V

I

R

R

G

G

E

E

V

V

K

P

P

E

E

I

Q

L

L

V

A

S

A

A

A

I

L

L

V

V

S

A

M

L

K

R

V

M

R

K

G

G

E

E

H

S

P

K

Q

N

E

E

I

I

A

V

G

G

A

F

A

A

T

R

A

A

L

M

L

R

E

E

N

L

A

A

A

I

P

K

F

I

P

D

R

V

P

P

D

N

Y

A

L

I

F

-

A

-

D

D

I

T

V
x
A

G
|
G

T

T

G

G

D
|
D

G

G

S

L

N

G

S
x
T

I

V

N
|
N

I

V

S
|
S

T
|
T

A

A

S

S

A

A

F

I

V

L

A

L

A

S

A

L

C

V

G

N

L

-

K

P

V

V

A

A

K
|
K

H

H

G

G

N

N

R

R

S

A

V

V

S

S

S

G

K

K

S

S

G

G

S
|
S

S

A

D

D

L

V

L

L

A

E

A

A

F

L

G

G

I

Y

N

N

L

I

D

I

M

V

N

P

A

P

D

E

K

R

S

A

R

K

Q

E

A

L

L

V

D

N

E

K

L

T

G

N

V

F

C

V

F

F

L

L

F

F

A

A

P

Q

K

Y

Y

Y

H

H

T

P

G

A

F

M

R

K

H

N

A

V

M

A

P

N

V

V

R

R

Q

K

Q

T

L

L

K

G

T

I

R

R

T

T

L

I

F

F

N

N

V

I

L

L

G

G

P

P

L

L

I

T

N

N

P

P

A

A

H

N

P

A

P

K

L

Y

A

Q

L

L

I

M

G

G

V

V

Y

F

S

S

P

K

E

D

L

H

V

L

L

D

P

L

I

L

A

S

E

K

T

S

L

A

R

Y

V

E

L

L

G

D

Y

F

Q

N

R

K

A

I

V

L

V

V

H

Y

S

G

G

E

-

P

G

G

M

I

D
|
D

E
|
E

V

V

S

S

L

P

H

I

A

G

P

N

T

T

V

F

V

M

A

K

E

I

L

V

N

S

N

K

G

R

E

G

I

I

K

E

S

E

Y

V

Q

K

L

L

T

N

A

V

A

T

D

D

F

F

G

G

L

I

T

S

P

P

Y

I

H

P

Q

I

E

E

Q

K

L

L

A

I

G

V

G

N

T

S

P

A

E

E

E

D

N

S

R

A

D

I

I

K

L

I

T

V

R

R

L

A

L

F

Q

L

G

G

K

K

G

D

E

E

A

H

A

V

H

A

E

E

binding magnesium ion: D223 (= D422), E224 (= E423)
binding 1-O-pyrophosphono-5-O-phosphono-alpha-D-ribofuranose: A78 (≠ V277), G79 (= G278), D83 (= D282), T87 (≠ S286), N89 (= N288), S91 (= S290), T92 (= T291), K106 (= K306), S118 (= S318), D223 (= D422), E224 (= E423)

2gvqD Anthranilate phosphoribosyl-transferase (trpd) from s. Solfataricus in complex with anthranilate (see paper)
35% identity, 54% coverage: 199:485/531 of query aligns to 2:286/345 of 2gvqD

query
sites
2gvqD

T

N

L

I

Q

N

P

E

I

I

L

L

E

K

K

K

L

L

Y

I

Q

N

A

K

E

S

T

D

L

L

S

E

Q

I

Q

N

E

E

S

A

H

E

Q

E

L

L

F

A

S

K

A

A

V

I

R

R

G

G

E

E

V

V

K

P

P

E

E

I

Q

L

L

V

A

S

A

A

A

I

L

L

V

V

S

A

M

L

K

R

V

M

R

K

G

G

E

E

H

S

P

K

Q

N

E

E

I

I

A

V

G

G

A

F

A

A

T

R

A

A

L

M

L

R

E

E

N

L

A

A

A

I

P

K

F

I

P

D

R

V

P

P

D

N

Y

A

L

I

F

-

A

-

D

D

I

T

V

A

G

G

T

T

G

G

D

D

G

G

S

L

N

G

S

T

I

V

N

N

I

V

S

S

T

T

A

A

S

S

A

A

F

I

V

L

A

L

A

S

A

L

C

V

G

N

L

-

K

P

V

V

A

A

K

K

H
|
H

G
|
G

N
|
N

R

R

S

A

V

V

S

S

S

G

K

K

S

S

G

G

S

S

S

A

D

D

L

V

L

L

A

E

A

A

F

L

G

G

I

Y

N

N

L

I

D

I

M

V

N

P

A

P

D

E

K

R

S

A

R

K

Q

E

A

L

L

V

D

N

E

K

L

T

G

N

V

F

C

V

F

F

L

L

F

F

A

A

P
x
Q

K

Y

Y

Y

H
|
H

T

P

G

A

F
x
M

R

K

H

N

A

V

M

A

P

N

V

V

R
|
R

Q

K

Q

T

L

L

K

G

T

I

R

R

T

T

L

I

F

F

N

N

V

I

L

L

G
|
G

P
|
P

L

L

I

T

N

N

P

P

A

A

H

N

P

A

P

K

L

Y

A

Q

L

L

I

M

G

G

V

V

Y

F

S

S

P

K

E

D

L

H

V

L

L

D

P

L

I

L

A

S

E

K

T

S

L

A

R

Y

V

E

L

L

G

D

Y

F

Q

N

R

K

A

I

V

L

V

V

H

Y

S

G

G

E

-

P

G

G

M

I

D

D

E

E

V

V

S

S

L

P

H

I

A

G

P

N

T

T

V

F

V

M

A

K

E

I

L

V

N

S

N

K

G

R

E

G

I

I

K

E

S

E

Y

V

Q

K

L

L

T

N

A

V

A

T

D

D

F

F

G

G

L

I

T

S

P

P

Y

I

H

P

Q

I

E

E

Q

K

L

L

A

I

G

V

G

N

T

S

P

A

E

E

E

D

N

S

R

A

D

I

I

K

L

I

T

V

R

R

L

A

L

F

Q

L

G

G

K

K

G

D

E

E

A

H

A

V

H

A

E

E

binding 2-aminobenzoic acid: H107 (= H307), G108 (= G308), N109 (= N309), N109 (= N309), Q151 (≠ P351), H154 (= H354), M157 (≠ F357), R164 (= R364), G177 (= G377), P178 (= P378)

P50384 Anthranilate phosphoribosyltransferase; EC 2.4.2.18 from Saccharolobus solfataricus (strain ATCC 35092 / DSM 1617 / JCM 11322 / P2) (Sulfolobus solfataricus) (see 2 papers)
35% identity, 54% coverage: 199:485/531 of query aligns to 2:286/345 of P50384

query
sites
P50384

T

N

L

I

Q

N

P

E

I

I

L

L

E

K

K

K

L

L

Y

I

Q

N

A

K

E

S

T

D

L

L

S

E

Q

I

Q

N

E

E

S

A

H

E

Q

E

L

L

F

A

S

K

A

A

V

I

R

R

G

G

E

E

V

V

K

P

P

E

E

I

Q

L

L

V

A

S

A

A

A

I

L

L

V

V

S

A

M

L

K

R

V

M

R

K

G

G

E

E

H

S

P

K

Q

N

E

E

I

I

A

V

G

G

A

F

A

A

T

R

A

A

L

M

L

R

E

E

N

L

A

A

A

I

P

K

F

I

P

D

R

V

P

P

D

N

Y

A

L

I

F

-

A

-

D

D

I

T

V

A

G

G

T

T

G

G

D

D

G

G

S

L

N

G

S
x
T

I

V

N

N

I

V

S

S

T

T

A

A

S

S

A

A

F

I

V

L

A

L

A

S

A

L

C

V

G

N

L

-

K

P

V

V

A

A

K
|
K

H
|
H

G

G

N

N

R

R

S

A

V

V

S

S

S

G

K

K

S

S

G

G

S
|
S

S

A

D

D

L

V

L

L

A

E

A

A

F

L

G

G

I

Y

N

N

L

I

D

I

M

V

N

P

A

P

D

E

K

R

S

A

R

K

Q

E

A

L

L

V

D

N

E

K

L

T

G

N

V

F

C

V

F

F

L

L

F

F

A

A

P

Q

K

Y

Y

Y

H
|
H

T

P

G

A

F

M

R

K

H

N

A

V

M

A

P

N

V

V

R
|
R

Q

K

Q

T

L

L

K

G

T

I

R

R

T

T

L

I

F

F

N

N

V

I

L

L

G

G

P

P

L

L

I

T

N

N

P

P

A

A

H

N

P

A

P

K

L

Y

A

Q

L

L

I

M

G

G

V

V

Y

F

S

S

P

K

E

D

L

H

V

L

L

D

P

L

I

L

A

S

E

K

T

S

L

A

R

Y

V

E

L

L

G

D

Y

F

Q

N

R

K

A

I

V

L

V

V

H

Y

S

G

G

E

-

P

G

G

M

I

D
|
D

E
|
E

V

V

S

S

L

P

H

I

A

G

P

N

T

T

V

F

V

M

A

K

E

I

L

V

N

S

N

K

G

R

E

G

I

I

K

E

S

E

Y

V

Q

K

L

L

T

N

A

V

A

T

D

D

F

F

G

G

L

I

T

S

P

P

Y

I

H

P

Q

I

E

E

Q

K

L

L

A

I

G

V

G

N

T

S

P

A

E

E

E

D

N

S

R

A

D

I

I

K

L

I

T

V

R

R

L

A

L

F

Q

L

G

G

K

K

G

D

E

E

A

H

A

V

H

A

E

E

T87 (≠ S286) binding
K106 (= K306) mutation to Q: Affinity for phosphoribosylpyrophosphate is similar to that of the wild-type enzyme and catalytic efficiency dedreases only 10-fold.
H107 (= H307) mutation to A: Limited effect on either affinity for anthranilate and catalytic efficiency. 300-fold decrease of the affinity for anthranilate, whereas catalytic efficiency remains nearly unchanged; when associated with A-178.
S118 (= S318) binding
H154 (= H354) mutation to A: Limited effect on either affinity for anthranilate and catalytic efficiency.
R164 (= R364) mutation to A: Strong decrease of the affinity for anthranilate, although only a moderate 7-fold decrease in catalytic efficiency.
D223 (= D422) mutation to N: Affinity for phosphoribosylpyrophosphate is similar to that of the wild-type enzyme and catalytic efficiency is unchanged.
E224 (= E423) mutation to Q: Affinity for phosphoribosylpyrophosphate is similar to that of the wild-type enzyme and catalytic efficiency is unchanged.

1gxbA Anthranilate phosphoribosyltransferase in complex with pyrophosphate and magnesium (see paper)
34% identity, 54% coverage: 199:485/531 of query aligns to 2:282/339 of 1gxbA

query
sites
1gxbA

T

N

L

I

Q

N

P

E

I

I

L

L

E

K

K

K

L

L

Y

I

Q

N

A

K

E

S

T

D

L

L

S

E

Q

I

Q

N

E

E

S

A

H

E

Q

E

L

L

F

A

S

K

A

A

V

I

R

R

G

G

E

E

V

V

K

P

P

E

E

I

Q

L

L

V

A

S

A

A

A

I

L

L

V

V

S

A

M

L

K

R

V

M

R

K

G

G

E

E

H

S

P

K

Q

N

E

E

I

I

A

V

G

G

A

F

A

A

T

R

A

A

L

M

L

R

E

E

N

L

A

A

A

I

P

K

F

I

P

D

R

V

P

P

D

N

Y

A

L

-

F

-

A

-

D

-

I

-

V

I

G

D

T

T

G

A

G

G

D

-

G

G

S

L

N

G

S

T

I

V

N
|
N

I

V

S
|
S

T
|
T

A

A

S

S

A

A

F

I

V

L

A

L

A

S

A

L

C

V

G

N

L

-

K

P

V

V

A

A

K
|
K

H

H

G

G

N

N

R

R

S

A

V

V

S

S

S

G

K

K

S

S

G

G

S
|
S

S

A

D

D

L

V

L

L

A

E

A

A

F

L

G

G

I

Y

N

N

L

I

D

I

M

V

N

P

A

P

D

E

K

R

S

A

R

K

Q

E

A

L

L

V

D

N

E

K

L

T

G

N

V

F

C

V

F

F

L

L

F

F

A

A

P

Q

K

Y

Y

Y

H

H

T

P

G

A

F

M

R

K

H

N

A

V

M

A

P

N

V

V

R

R

Q

K

Q

T

L

L

K

G

T

I

R

R

T

T

L

I

F

F

N

N

V

I

L

L

G

G

P

P

L

L

I

T

N

N

P

P

A

A

H

N

P

A

P

K

L

Y

A

Q

L

L

I

M

G

G

V

V

Y

F

S

S

P

K

E

D

L

H

V

L

L

D

P

L

I

L

A

S

E

K

T

S

L

A

R

Y

V

E

L

L

G

D

Y

F

Q

N

R

K

A

I

V

L

V

V

H

Y

S

G

G

E

-

P

G

G

M

I

D

D

E
|
E

V

V

S

S

L

P

H

I

A

G

P

N

T

T

V

F

V

M

A

K

E

I

L

V

N

S

N

K

G

R

E

G

I

I

K

E

S

E

Y

V

Q

K

L

L

T

N

A

V

A

T

D

D

F

F

G

G

L

I

T

S

P

P

Y

I

H

P

Q

I

E

E

Q

K

L

L

A

I

G

V

G

N

T

S

P

A

E

E

E

D

N

S

R

A

D

I

I

K

L

I

T

V

R

R

L

A

L

F

Q

L

G

G

K

K

G

D

E

E

A

H

A

V

H

A

E

E

binding magnesium ion: S87 (= S290), E220 (= E423)
binding pyrophosphate 2-: N85 (= N288), S87 (= S290), T88 (= T291), K102 (= K306), S114 (= S318)

P00903 Aminodeoxychorismate synthase component 2; ADC synthase; ADCS; 4-amino-4-deoxychorismate synthase component 2; Aminodeoxychorismate synthase, glutamine amidotransferase component; EC 2.6.1.85 from Escherichia coli (strain K12) (see paper)
44% identity, 35% coverage: 4:187/531 of query aligns to 2:185/187 of P00903

query
sites
P00903

I

I

L

L

L

I

D

D

N

N

I

Y

D

D

S

S

F

F

T

T

Y

W

N

N

L

L

A

Y

D

Q

Q

Y

L

F

R

C

S

E

N

L

G

G

H

A

N

D

V

V

V

L

I

V

Y

K

R

R

N

N

S

D

V

A

P

L

A

T

Q

L

A

A

L

D

I

I

E

D

R

A

I

L

G

K

T

P

M

Q

K

K

N

-

P

-

V

-

L

I

M

V

L

I

S

S

P

P

G

G

P

P

G

C

T

T

P

P

S

D

E

E

A

A

G

G

C

I

M

S

P

L

E

D

L

V

L

I

I

R

R

H

M

Y

R

A

G

G

K

R

L

L

P

P

I

I

I

L

G

G

I

V

C
|
C

L

L

G

G

H

H

Q

Q

A

A

I

M

V

A

E

Q

A

A

Y

F

G

G

Y

K

V

V

G

V

Q

R

A

A

G

A

E

K

I

V

L

M

H

H

G

G

K

K

A

T

S

S

S

P

I

I

E

T

H

H

D

N

G

G

Q

E

A

G

M

V

F

F

A

R

G

G

L

L

S

A

N

N

P

P

L

L

P

T

V

V

A

T

R

R

Y

Y

H

H

S

S

L

L

V

V

-

V

-

E

G

P

S

D

N

S

I

L

P

P

A

A

G

C

L

F

T

D

I

V

N

T

A

A

H

W

F

S

N

E

G

T

M

R

-

E

V

I

M

M

A

G

V

I

R

R

H

H

D

R

A

Q

D

W

R

D

V

L

C

E

G

G

F

V

Q

Q

F

F

H
|
H

P

P

E
|
E

S

S

I

I

L

L

T

S

T

E

Q

Q

G

G

A

H

R

Q

L

L

E

A

Q

N

T

F

L

L

C79 (= C84) mutation to S: 10000-fold decrease in catalytic efficiency.
H168 (= H170) mutation to Q: Loss of activity.
E170 (= E172) mutation to A: 150-fold decrease in catalytic efficiency.; mutation to D: 4-fold decrease in catalytic efficiency.; mutation E->K,Q: Loss of activity.

4owoA Anthranilate phosphoribosyl transferase from mycobacterium tuberculosis in complex with 6-fluoroanthranilate, prpp and magnesium (see paper)
37% identity, 49% coverage: 203:463/531 of query aligns to 8:270/348 of 4owoA

query
sites
4owoA

I

I

L

L

E

G

K

R

L

L

Y

T

Q

D

A

N

E

R

T

D

L

L

S

A

Q

R

Q

G

E

Q

S

A

H

A

Q

W

L

A

F

M

S

D

A

Q

V

I

V

M

R

T

G

G

E

N

V

A

K

R

P

P

E

A

Q

Q

L

I

A

A

A

F

L

A

V

V

S

A

M

M

K

T

V

M

R

K

G

A

E

P

H

T

P

A

Q

D

E

E

I

V

A

G

G

E

A

L

A

A

T

G

A

V

L

M

L

L

E

S

N

H

A

A

A

H

P

P

F

L

P

P

R

A

-

D

-

T

-

V

P

P

D

D

Y

D

L

A

F

V

A

-

D

D

I

V

V
|
V

G
|
G

T
|
T

G
|
G

D

D

G

G

S

V

N

N

S

T

I

V

N
|
N

I

L

S
|
S

T
|
T

A

M

S

A

A

A

F

I

V

V

A

V

A

A

C

A

G

G

L

V

K

P

V

V

A

V

K
|
K

H
|
H

G

G

N
|
N

R

R

S
x
A

V
x
A

S
|
S

K

L

S

S

G
|
G

S

G

S

A

D

D

L

T

L

L

A

E

A

A

F

L

G

G

I

V

N

R

L

I

D

D

M

L

N

G

A

P

D

D

K

L

S

V

R

A

Q

R

A

S

L

L

D

A

E

E

L

V

G

G

V

I

C

G

F

F

L

C

F

F

A
|
A

P
|
P

K

R

Y

F

H
|
H

T

P

G

S

F
x
Y

R

R

H

H

A

A

M
x
A

P

A

V

V

R
|
R

Q

R

Q

E

L

I

K

G

T

V

R

P

T

T

L

V

F

F

N

N

V

L

L

L

G
|
G

P

P

L

L

I

T

N

N

P

P

A

A

H

R

P

P

P

R

L

A

A

G

L

L

I

I

G

G

V

C

Y

A

S

F

P

A

E

D

L

L

V

A

L

E

P

V

I

M

A

A

E

G

T

-

L

-

R

-

V

V

L

F

G

A

Y

A

Q

R

R

R

A

S

-

V

-

L

V

V

H

H

-

G

S

D

G

D

G

G

M

L

D
|
D

E
|
E

V

L

S

T

L

T

H

T

A

T

P

T

T

S

V

T

V

I

A

W

E

R

L

V

N

A

G

G

E

S

I

V

K

D

S

K

Y

L

Q

T

L

F

T

D

A

P

A

A

D

G

F

F

G

G

L

F

T

A

P

R

Y

A

H

Q

Q

L

E

D

Q

Q

L

L

A

A

G

G

active site: V84 (= V277)
binding 2-azanyl-6-fluoranyl-benzoic acid: V84 (= V277), G85 (= G278), T86 (= T279), H114 (= H307), N116 (= N309), N116 (= N309), A157 (= A350), P158 (= P351), H161 (= H354), Y164 (≠ F357), A168 (≠ M361), R171 (= R364), G184 (= G377)
binding magnesium ion: S97 (= S290), D229 (= D422), E230 (= E423), E230 (= E423)
binding 1-O-pyrophosphono-5-O-phosphono-alpha-D-ribofuranose: V84 (= V277), G85 (= G278), G87 (= G280), G88 (= G281), N95 (= N288), S97 (= S290), T98 (= T291), K113 (= K306), A118 (≠ S311), A119 (≠ V312), S120 (= S313), S121 (= S314), G124 (= G317)

5c1rA Stereoisomer of prpp bound in the active site of mycobacterium tuberculosis anthranilate phosphoribosyl (anprt; trpd)
37% identity, 49% coverage: 203:463/531 of query aligns to 6:268/343 of 5c1rA

query
sites
5c1rA

I

I

L

L

E

G

K

R

L

L

Y

T

Q

D

A

N

E

R

T

D

L

L

S

A

Q

R

Q

G

E

Q

S

A

H

A

Q

W

L

A

F

M

S

D

A

Q

V

I

V

M

R

T

G

G

E

N

V

A

K

R

P

P

E

A

Q

Q

L

I

A

A

A

F

L

A

V

V

S

A

M

M

K

T

V

M

R

K

G

A

E

P

H

T

P

A

Q

D

E

E

I

V

A

G

G

E

A

L

A

A

T

G

A

V

L

M

L

L

E

S

N

H

A

A

A

H

P

P

F

L

P

P

R

A

-

D

-

T

-

V

P

P

D

D

Y

D

L

A

F

V

A

-

D

D

I

V

V
|
V

G
|
G

T

T

G

G

G
|
G

D
|
D

G

G

S
x
V

N

N

S

T

I

V

N
|
N

I

L

S
|
S

T
|
T

A

M

S

A

A

A

F

I

V

V

A

V

A

A

C

A

G

G

L

V

K

P

V

V

A

V

K
|
K

H

H

G

G

N

N

R
|
R

S

A

V
x
A

S
|
S

K

L

S

S

G

G

S

G

S

A

D

D

L

T

L

L

A

E

A

A

F

L

G

G

I

V

N

R

L

I

D

D

M

L

N

G

A

P

D

D

K

L

S

V

R

A

Q

R

A

S

L

L

D

A

E

E

L

V

G

G

V

I

C

G

F

F

L

C

F

F

A

A

P

P

K

R

Y

F

H

H

T

P

G

S

F

Y

R

R

H

H

A

A

M

A

P

A

V

V

R

R

Q

R

Q

E

L

I

K

G

T

V

R

P

T

T

L

V

F

F

N

N

V

L

L

L

G

G

P

P

L

L

I

T

N

N

P

P

A

A

H

R

P

P

P

R

L

A

A

G

L

L

I

I

G

G

V

C

Y

A

S

F

P

A

E

D

L

L

V

A

L

E

P

V

I

M

A

A

E

G

T

-

L

-

R

-

V

V

L

F

G

A

Y

A

Q

R

R

R

A

S

-

V

-

L

V

V

H

H

-

G

S

D

G

D

G

G

M

L

D

D

E
|
E

V

L

S

T

L

T

H

T

A

T

P

T

T

S

V

T

V

I

A

W

E

R

L

V

N

A

G

G

E

S

I

V

K

D

S

K

Y

L

Q

T

L

F

T

D

A

P

A

A

D

G

F

F

G

G

L

F

T

A

P

R

Y

A

H

Q

Q

L

E

D

Q

Q

L

L

A

A

G

G

active site: V82 (= V277)
binding 5-O-[(R)-hydroxy(phosphonooxy)phosphoryl]-1-O-phosphono-alpha-D-ribofuranose: V82 (= V277), G83 (= G278), G86 (= G281), N93 (= N288), S95 (= S290), T96 (= T291), K111 (= K306), R115 (= R310), A117 (≠ V312), S118 (= S313), S119 (= S314)
binding magnesium ion: D87 (= D282), V89 (≠ S284), S95 (= S290), E228 (= E423)

5c2lA Magnesium soaked into the active site of mycobacterium tuberculosis anthranilate phosphoribosyltransferase (anprt; trpd)
37% identity, 49% coverage: 203:463/531 of query aligns to 8:270/349 of 5c2lA

query
sites
5c2lA

I

I

L

L

E

G

K

R

L

L

Y

T

Q

D

A

N

E

R

T

D

L

L

S

A

Q

R

Q

G

E

Q

S

A

H

A

Q

W

L

A

F

M

S

D

A

Q

V

I

V

M

R

T

G

G

E

N

V

A

K

R

P

P

E

A

Q

Q

L

I

A

A

A

F

L

A

V

V

S

A

M

M

K

T

V

M

R

K

G

A

E

P

H

T

P

A

Q

D

E

E

I

V

A

G

G

E

A

L

A

A

T

G

A

V

L

M

L

L

E

S

N

H

A

A

A

H

P

P

F

L

P

P

R

A

-

D

-

T

-

V

P

P

D

D

Y

D

L

A

F

V

A

-

D

D

I

V

V
|
V

G

G

T

T

G

G

D

D

G

G

S

V

N

N

S

T

I

V

N

N

I

L

S

S

T

T

A

M

S

A

A

A

F

I

V

V

A

V

A

A

C

A

G

G

L

V

K

P

V

V

A

V

K

K

H

H

G

G

N

N

R

R

S

A

V

A

S

S

K

L

S

S

G

G

S

G

S

A

D

D

L

T

L

L

A

E

A

A

F

L

G

G

I

V

N

R

L

I

D

D

M

L

N

G

A

P

D

D

K

L

S

V

R

A

Q

R

A

S

L

L

D

A

E

E

L

V

G

G

V

I

C

G

F

F

L

C

F

F

A

A

P

P

K

R

Y

F

H

H

T

P

G

S

F

Y

R

R

H

H

A

A

M

A

P

A

V

V

R

R

Q

R

Q

E

L

I

K

G

T

V

R

P

T

T

L

V

F

F

N

N

V

L

L

L

G

G

P

P

L

L

I

T

N

N

P

P

A

A

H

R

P

P

P

R

L

A

A

G

L

L

I

I

G

G

V

C

Y

A

S

F

P

A

E

D

L

L

V

A

L

E

P

V

I

M

A

A

E

G

T

-

L

-

R

-

V

V

L

F

G

A

Y

A

Q

R

R

R

A

S

-

V

-

L

V

V

H

H

-

G

S

D

G

D

G

G

M

L

D
|
D

E
|
E

V

L

S

T

L

T

H

T

A

T

P

T

T

S

V

T

V

I

A

W

E

R

L

V

N

A

G

G

E

S

I

V

K

D

S

K

Y

L

Q

T

L

F

T

D

A

P

A

A

D

G

F

F

G

G

L

F

T

A

P

R

Y

A

H

Q

Q

L

E

D

Q

Q

L

L

A

A

G

G

active site: V84 (= V277)
binding magnesium ion: D229 (= D422), E230 (= E423)

4owuA Anthranilate phosphoribosyl transferase from mycobacterium tuberculosis in complex with 5-methylanthranilate, prpp and magnesium (see paper)
37% identity, 49% coverage: 203:463/531 of query aligns to 7:269/347 of 4owuA

query
sites
4owuA

I

I

L

L

E

G

K

R

L

L

Y

T

Q

D

A

N

E

R

T

D

L

L

S

A

Q

R

Q

G

E

Q

S

A

H

A

Q

W

L

A

F

M

S

D

A

Q

V

I

V

M

R

T

G

G

E

N

V

A

K

R

P

P

E

A

Q

Q

L

I

A

A

A

F

L

A

V

V

S

A

M

M

K

T

V

M

R

K

G

A

E

P

H

T

P

A

Q

D

E

E

I

V

A

G

G

E

A

L

A

A

T

G

A

V

L

M

L

L

E

S

N

H

A

A

A

H

P

P

F

L

P

P

R

A

-

D

-

T

-

V

P

P

D

D

Y

D

L

A

F

V

A

-

D

D

I

V

V
|
V

G
|
G

T

T

G
|
G

D

D

G

G

S

V

N

N

S

T

I

V

N
|
N

I

L

S
|
S

T
|
T

A

M

S

A

A

A

F

I

V

V

A

V

A

A

C

A

G

G

L

V

K

P

V

V

A

V

K
|
K

H

H

G

G

N
|
N

R

R

S

A

V
x
A

S

S

S
|
S

K

L

S

S

G
|
G

S

G

S

A

D

D

L

T

L

L

A

E

A

A

F

L

G

G

I

V

N

R

L

I

D

D

M

L

N

G

A

P

D

D

K

L

S

V

R

A

Q

R

A

S

L

L

D

A

E

E

L

V

G

G

V

I

C

G

F

F

L

C

F

F

A

A

P
|
P

K

R

Y

F

H

H

T

P

G

S

F
x
Y

R
|
R

H

H

A

A

M
x
A

P

A

V

V

R

R

Q

R

Q

E

L

I

K

G

T

V

R

P

T

T

L

V

F

F

N

N

V

L

L

L

G

G

P

P

L

L

I

T

N

N

P

P

A

A

H

R

P

P

P

R

L

A

A

G

L

L

I

I

G

G

V

C

Y

A

S

F

P

A

E

D

L

L

V

A

L

E

P

V

I

M

A

A

E

G

T

-

L

-

R

-

V

V

L

F

G

A

Y

A

Q

R

R

R

A

S

-

V

-

L

V

V

H

H

-

G

S

D

G

D

G

G

M

L

D
|
D

E
|
E

V

L

S

T

L

T

H

T

A

T

P

T

T

S

V

T

V

I

A

W

E

R

L

V

N

A

G

G

E

S

I

V

K

D

S

K

Y

L

Q

T

L

F

T

D

A

P

A

A

D

G

F

F

G

G

L

F

T

A

P

R

Y

A

H

Q

Q

L

E

D

Q

Q

L

L

A

A

G

G

active site: V83 (= V277)
binding 2-azanyl-5-methyl-benzoic acid: N115 (= N309), P157 (= P351), Y163 (≠ F357), R164 (= R358), A167 (≠ M361)
binding magnesium ion: S96 (= S290), D228 (= D422), E229 (= E423), E229 (= E423)
binding 1-O-pyrophosphono-5-O-phosphono-alpha-D-ribofuranose: G84 (= G278), G86 (= G280), G87 (= G281), N94 (= N288), S96 (= S290), T97 (= T291), K112 (= K306), A118 (≠ V312), S120 (= S314), G123 (= G317)

4owqA Anthranilate phosphoribosyl transferase from mycobacterium tuberculosis in complex with 3-methylanthranilate, prpp and magnesium (see paper)
37% identity, 49% coverage: 203:463/531 of query aligns to 7:269/347 of 4owqA

query
sites
4owqA

I

I

L

L

E

G

K

R

L

L

Y

T

Q

D

A

N

E

R

T

D

L

L

S

A

Q

R

Q

G

E

Q

S

A

H

A

Q

W

L

A

F

M

S

D

A

Q

V

I

V

M

R

T

G

G

E

N

V

A

K

R

P

P

E

A

Q

Q

L

I

A

A

A

F

L

A

V

V

S

A

M

M

K

T

V

M

R

K

G

A

E

P

H

T

P

A

Q

D

E

E

I

V

A

G

G

E

A

L

A

A

T

G

A

V

L

M

L

L

E

S

N

H

A

A

A

H

P

P

F

L

P

P

R

A

-

D

-

T

-

V

P

P

D

D

Y

D

L

A

F

V

A

-

D

D

I

V

V
|
V

G
|
G

T

T

G
|
G

D

D

G

G

S

V

N

N

S

T

I

V

N
|
N

I

L

S
|
S

T
|
T

A

M

S

A

A

A

F

I

V

V

A

V

A

A

C

A

G

G

L

V

K

P

V

V

A

V

K
|
K

H

H

G

G

N

N

R

R

S
x
A

V
x
A

S

S

S
|
S

K

L

S

S

G
|
G

S

G

S

A

D

D

L

T

L

L

A

E

A

A

F

L

G

G

I

V

N

R

L

I

D

D

M

L

N

G

A

P

D

D

K

L

S

V

R

A

Q

R

A

S

L

L

D

A

E

E

L

V

G

G

V

I

C

G

F

F

L

C

F

F

A

A

P
|
P

K

R

Y

F

H
|
H

T

P

G

S

F
x
Y

R

R

H

H

A

A

M
x
A

P

A

V

V

R

R

Q
x
R

Q

E

L

I

K

G

T

V

R

P

T

T

L

V

F

F

N

N

V

L

L

L

G

G

P

P

L

L

I

T

N

N

P

P

A

A

H

R

P

P

P

R

L

A

A

G

L

L

I

I

G

G

V

C

Y

A

S

F

P

A

E

D

L

L

V

A

L

E

P

V

I

M

A

A

E

G

T

-

L

-

R

-

V

V

L

F

G

A

Y

A

Q

R

R

R

A

S

-

V

-

L

V

V

H

H

-

G

S

D

G

D

G

G

M

L

D
|
D

E
|
E

V

L

S

T

L

T

H

T

A

T

P

T

T

S

V

T

V

I

A

W

E

R

L

V

N

A

G

G

E

S

I

V

K

D

S

K

Y

L

Q

T

L

F

T

D

A

P

A

A

D

G

F

F

G

G

L

F

T

A

P

R

Y

A

H

Q

Q

L

E

D

Q

Q

L

L

A

A

G

G

active site: V83 (= V277)
binding 2-azanyl-3-methyl-benzoic acid: P157 (= P351), H160 (= H354), Y163 (≠ F357), A167 (≠ M361), R171 (≠ Q365)
binding magnesium ion: S96 (= S290), D228 (= D422), E229 (= E423), E229 (= E423)
binding 1-O-pyrophosphono-5-O-phosphono-alpha-D-ribofuranose: V83 (= V277), G84 (= G278), G86 (= G280), G87 (= G281), N94 (= N288), S96 (= S290), T97 (= T291), K112 (= K306), A117 (≠ S311), A118 (≠ V312), S120 (= S314), G123 (= G317)

4n5vA Alternative substrates of mycobacterium tuberculosis anthranilate phosphoribosyl transferase (see paper)
37% identity, 49% coverage: 203:463/531 of query aligns to 8:270/347 of 4n5vA

query
sites
4n5vA

I

I

L

L

E

G

K

R

L

L

Y

T

Q

D

A

N

E

R

T

D

L

L

S

A

Q

R

Q

G

E

Q

S

A

H

A

Q

W

L

A

F

M

S

D

A

Q

V

I

V

M

R

T

G

G

E

N

V

A

K

R

P

P

E

A

Q

Q

L

I

A

A

A

F

L

A

V

V

S

A

M

M

K

T

V

M

R

K

G

A

E

P

H

T

P

A

Q

D

E

E

I

V

A

G

G

E

A

L

A

A

T

G

A

V

L

M

L

L

E

S

N

H

A

A

A

H

P

P

F

L

P

P

R

A

-

D

-

T

-

V

P

P

D

D

Y

D

L

A

F

V

A

-

D

D

I

V

V
|
V

G
|
G

T

T

G
|
G

D

D

G

G

S

V

N

N

S

T

I

V

N
|
N

I

L

S
|
S

T
|
T

A

M

S

A

A

A

F

I

V

V

A

V

A

A

C

A

G

G

L

V

K

P

V

V

A

V

K
|
K

H
|
H

G
|
G

N
|
N

R

R

S

A

V
x
A

S
|
S

K

L

S

S

G
|
G

S

G

S

A

D

D

L

T

L

L

A

E

A

A

F

L

G

G

I

V

N

R

L

I

D

D

M

L

N

G

A

P

D

D

K

L

S

V

R

A

Q

R

A

S

L

L

D

A

E

E

L

V

G

G

V

I

C

G

F

F

L

C

F

F

A
|
A

P
|
P

K

R

Y

F

H
|
H

T

P

G

S

F
x
Y

R
|
R

H

H

A

A

M
x
A

P

A

V

V

R
|
R

Q

R

Q

E

L

I

K

G

T

V

R

P

T

T

L

V

F

F

N

N

V

L

L

L

G
|
G

P

P

L

L

I

T

N

N

P

P

A

A

H

R

P

P

P

R

L

A

A

G

L

L

I

I

G

G

V

C

Y

A

S

F

P

A

E

D

L

L

V

A

L

E

P

V

I

M

A

A

E

G

T

-

L

-

R

-

V

V

L

F

G

A

Y

A

Q

R

R

R

A

S

-

V

-

L

V

V

H

H

-

G

S

D

G

D

G

G

M

L

D
|
D

E
|
E

V

L

S

T

L

T

H

T

A

T

P

T

T

S

V

T

V

I

A

W

E

R

L

V

N

A

G

G

E

S

I

V

K

D

S

K

Y

L

Q

T

L

F

T

D

A

P

A

A

D

G

F

F

G

G

L

F

T

A

P

R

Y

A

H

Q

Q

L

E

D

Q

Q

L

L

A

A

G

G

active site: V84 (= V277)
binding 2-amino-4-fluorobenzoic acid: V84 (= V277), G85 (= G278), H114 (= H307), G115 (= G308), N116 (= N309), A157 (= A350), A157 (= A350), P158 (= P351), H161 (= H354), Y164 (≠ F357), R165 (= R358), A168 (≠ M361), R171 (= R364), G184 (= G377)
binding magnesium ion: S97 (= S290), D229 (= D422), E230 (= E423), E230 (= E423)
binding 1-O-pyrophosphono-5-O-phosphono-alpha-D-ribofuranose: G85 (= G278), G87 (= G280), G88 (= G281), N95 (= N288), S97 (= S290), T98 (= T291), K113 (= K306), A119 (≠ V312), S120 (= S313), S121 (= S314), G124 (= G317)

P9WFX5 Anthranilate phosphoribosyltransferase; EC 2.4.2.18 from Mycobacterium tuberculosis (strain ATCC 25618 / H37Rv) (see 2 papers)
37% identity, 49% coverage: 203:463/531 of query aligns to 30:292/370 of P9WFX5

query
sites
P9WFX5

I

I

L

L

E

G

K

R

L

L

Y

T

Q

D

A

N

E

R

T

D

L

L

S

A

Q

R

Q

G

E

Q

S

A

H

A

Q

W

L

A

F

M

S

D

A

Q

V

I

V

M

R

T

G

G

E

N

V

A

K

R

P

P

E

A

Q

Q

L

I

A

A

A

F

L

A

V

V

S

A

M

M

K

T

V

M

R

K

G

A

E

P

H

T

P

A

Q

D

E

E

I

V

A

G

G

E

A

L

A

A

T

G

A

V

L

M

L

L

E

S

N

H

A

A

A

H

P

P

F

L

P

P

R

A

-

D

-

T

-

V

P

P

D

D

Y

D

L

A

F

V

A

-

D

D

I

V

V

V

G
|
G

T

T

G

G

D

D

G

G

S

V

N

N

S
x
T

I

V

N

N

I

L

S

S

T

T

A

M

S

A

A

A

F

I

V

V

A

V

A

A

C

A

G

G

L

V

K

P

V

V

A

V

K

K

H

H

G

G

N

N

R

R

S

A

V

A

S

S

K

L

S

S

G

G

S
x
G

S

A

D

D

L

T

L

L

A

E

A

A

F

L

G

G

I

V

N

R

L

I

D

D

M

L

N

G

A

P

D

D

K

L

S

V

R

A

Q

R

A

S

L

L

D

A

E

E

L

V

G

G

V

I

C

G

F

F

L

C

F

F

A

A

P

P

K

R

Y

F

H

H

T

P

G

S

F

Y

R

R

H

H

A

A

M

A

P

A

V

V

R

R

Q

R

Q

E

L

I

K

G

T

V

R

P

T

T

L

V

F

F

N

N

V

L

L

L

G

G

P

P

L

L

I

T

N

N

P

P

A

A

H

R

P

P

P

R

L

A

A

G

L

L

I

I

G

G

V

C

Y

A

S

F

P

A

E

D

L

L

V

A

L

E

P

V

I

M

A

A

E

G

T

-

L

-

R

-

V

V

L

F

G

A

Y

A

Q

R

R

R

A

S

-

V

-

L

V

V

H

H

-

G

S

D

G

D

G

G

M

L

D

D

E

E

V

L

S

T

L

T

H

T

A

T

P

T

T

S

V

T

V

I

A

W

E

R

L

V

N

A

G

G

E

S

I

V

K

D

S

K

Y

L

Q

T

L

F

T

D

A

P

A

A

D

G

F

F

G

G

L

F

T

A

P

R

Y

A

H

Q

Q

L

E

D

Q

Q

L

L

A

A

G

G

G107 (= G278) binding
T115 (≠ S286) binding
G147 (≠ S318) binding

4zokA Methylsulfonyl-containing inhibitor bound in the substrate capture site of mycobacterium tuberculosis anthranilate phosphoribosyltransferase (anprt; trpd)
37% identity, 49% coverage: 203:463/531 of query aligns to 6:268/342 of 4zokA

query
sites
4zokA

I

I

L

L

E

G

K

R

L

L

Y

T

Q

D

A

N

E

R

T

D

L

L

S

A

Q

R

Q

G

E

Q

S

A

H

A

Q

W

L

A

F

M

S

D

A

Q

V

I

V

M

R

T

G

G

E

N

V

A

K

R

P

P

E

A

Q

Q

L

I

A

A

A

F

L

A

V

V

S

A

M

M

K

T

V

M

R

K

G

A

E

P

H

T

P

A

Q

D

E

E

I

V

A

G

G

E

A

L

A

A

T

G

A

V

L

M

L

L

E

S

N

H

A

A

A

H

P

P

F

L

P

P

R

A

-

D

-

T

-

V

P

P

D

D

Y

D

L

A

F

V

A

-

D

D

I

V

V
|
V

G

G

T

T

G

G

G
|
G

D

D

G

G

S

V

N

N

S

T

I

V

N
|
N

I

L

S
|
S

T
|
T

A

M

S

A

A

A

F

I

V

V

A

V

A

A

C

A

G

G

L

V

K

P

V

V

A

V

K
|
K

H

H

G

G

N

N

R

R

S
x
A

V
x
A

S

S

S
|
S

K

L

S

S

G
|
G

S

G

S

A

D

D

L

T

L

L

A

E

A

A

F

L

G

G

I

V

N

R

L

I

D

D

M

L

N

G

A

P

D

D

K

L

S

V

R

A

Q

R

A

S

L

L

D

A

E

E

L

V

G

G

V

I

C

G

F

F

L

C

F

F

A

A

P
|
P

K

R

Y

F

H

H

T

P

G

S

F

Y

R
|
R

H

H

A

A

M
x
A

P
x
A

V

V

R

R

Q

R

Q

E

L

I

K

G

T

V

R

P

T

T

L

V

F

F

N

N

V

L

L

L

G

G

P

P

L

L

I

T

N

N

P

P

A

A

H

R

P

P

P

R

L

A

A

G

L

L

I

I

G

G

V

C

Y

A

S

F

P

A

E

D

L

L

V

A

L

E

P

V

I

M

A

A

E

G

T

-

L

-

R

-

V

V

L

F

G

A

Y

A

Q

R

R

R

A

S

-

V

-

L

V

V

H

H

-

G

S

D

G

D

G

G

M

L

D

D

E
|
E

V

L

S

T

L

T

H

T

A

T

P

T

T

S

V

T

V

I

A

W

E

R

L

V

N

A

G

G

E

S

I

V

K

D

S

K

Y

L

Q

T

L

F

T

D

A

P

A

A

D

G

F

F

G

G

L

F

T

A

P

R

Y

A

H

Q

Q

L

E

D

Q

Q

L

L

A

A

G

G

active site: V82 (= V277)
binding 4-methyl-2-{[2-methyl-6-(methylsulfonyl)phenyl]amino}benzoic acid: P156 (= P351), R163 (= R358), A166 (≠ M361), A167 (≠ P362)
binding magnesium ion: S95 (= S290), E228 (= E423)
binding 1-O-pyrophosphono-5-O-phosphono-alpha-D-ribofuranose: G86 (= G281), N93 (= N288), S95 (= S290), T96 (= T291), K111 (= K306), A116 (≠ S311), A117 (≠ V312), S119 (= S314), G122 (= G317)

4zofA Lobenzarit-like inhibitor bound in the active site of mycobacterium tuberculosis anthranilate phosphoribosyltransferase (anprt; trpd)
37% identity, 49% coverage: 203:463/531 of query aligns to 6:268/342 of 4zofA

query
sites
4zofA

I

I

L

L

E

G

K

R

L

L

Y

T

Q

D

A

N

E

R

T

D

L

L

S

A

Q

R

Q

G

E

Q

S

A

H

A

Q

W

L

A

F

M

S

D

A

Q

V

I

V

M

R

T

G

G

E

N

V

A

K

R

P

P

E

A

Q

Q

L

I

A

A

A

F

L

A

V

V

S

A

M

M

K

T

V

M

R

K

G

A

E

P

H

T

P

A

Q

D

E

E

I

V

A

G

G

E

A

L

A

A

T

G

A

V

L

M

L

L

E

S

N

H

A

A

A

H

P

P

F

L

P

P

R

A

-

D

-

T

-

V

P

P

D

D

Y

D

L

A

F

V

A

-

D

D

I

V

V
|
V

G
|
G

T

T

G
|
G

D

D

G

G

S

V

N

N

S

T

I

V

N
|
N

I

L

S
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S

T
|
T

A

M

S

A

A

A

F

I

V

V

A

V

A

A

C

A

G

G

L

V

K

P

V

V

A

V

K
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K

H

H

G
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G

N
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N

R

R

S
x
A

V
x
A

S

S

S
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S

K

L

S

S

G
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G

S

G

S

A

D

D

L

T

L

L

A

E

A

A

F

L

G

G

I

V

N

R

L

I

D

D

M

L

N

G

A

P

D

D

K

L

S

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R

A

Q

R

A

S

L

L

D

A

E

E

L

V

G

G

V

I

C

G

F

F

L

C

F

F

A
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A

P

P

K

R

Y

F

H
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H

T

P

G

S

F
x
Y

R

R

H

H

A

A

M

A

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A

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R
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R

Q

R

Q

E

L

I

K

G

T

V

R

P

T

T

L

V

F

F

N

N

V

L

L

L

G
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G

P

P

L

L

I

T

N

N

P

P

A

A

H

R

P

P

P

R

L

A

A

G

L

L

I

I

G

G

V

C

Y

A

S

F

P

A

E

D

L

L

V

A

L

E

P

V

I

M

A

A

E

G

T

-

L

-

R

-

V

V

L

F

G

A

Y

A

Q

R

R

R

A

S

-

V

-

L

V

V

H

H

-

G

S

D

G

D

G

G

M

L

D
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D

E
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E

V

L

S

T

L

T

H

T

A

T

P

T

T

S

V

T

V

I

A

W

E

R

L

V

N

A

G

G

E

S

I

V

K

D

S

K

Y

L

Q

T

L

F

T

D

A

P

A

A

D

G

F

F

G

G

L

F

T

A

P

R

Y

A

H

Q

Q

L

E

D

Q

Q

L

L

A

A

G

G

active site: V82 (= V277)
binding 2-[(2-carboxy-5-nitrophenyl)amino]-3-methylbenzoic acid: G113 (= G308), N114 (= N309), A155 (= A350), H159 (= H354), Y162 (≠ F357), R169 (= R364), G182 (= G377)
binding magnesium ion: S95 (= S290), D227 (= D422), E228 (= E423), E228 (= E423)
binding 1-O-pyrophosphono-5-O-phosphono-alpha-D-ribofuranose: G83 (= G278), G85 (= G280), G86 (= G281), N93 (= N288), S95 (= S290), T96 (= T291), K111 (= K306), A116 (≠ S311), A117 (≠ V312), S119 (= S314), G122 (= G317)

3r88B Anthranilate phosphoribosyltransferase (trpd) from mycobacterium tuberculosis (complex with inhibitor acs145) (see paper)
37% identity, 49% coverage: 203:463/531 of query aligns to 6:268/344 of 3r88B

query
sites
3r88B

I

I

L

L

E

G

K

R

L

L

Y

T

Q

D

A

N

E

R

T

D

L

L

S

A

Q

R

Q

G

E

Q

S

A

H

A

Q

W

L

A

F

M

S

D

A

Q

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I

V

M

R

T

G

G

E

N

V

A

K

R

P

P

E

A

Q

Q

L

I

A

A

A

F

L

A

V

V

S

A

M

M

K

T

V

M

R

K

G

A

E

P

H

T

P

A

Q

D

E

E

I

V

A

G

G

E

A

L

A

A

T

G

A

V

L

M

L

L

E

S

N

H

A

A

A

H

P

P

F

L

P

P

R

A

-

D

-

T

-

V

P

P

D

D

Y

D

L

A

F

V

A

-

D

D

I

V

V
|
V

G
|
G

T

T

G
|
G

D

D

G

G

S

V

N

N

S

T

I

V

N
|
N

I

L

S
|
S

T
|
T

A

M

S

A

A

A

F

I

V

V

A

V

A

A

C

A

G

G

L

V

K

P

V

V

A

V

K
|
K

H

H

G

G

N
|
N

R

R

S

A

V
x
A

S
|
S

K

L

S

S

G

G

S

G

S

A

D

D

L

T

L

L

A

E

A

A

F

L

G

G

I

V

N

R

L

I

D

D

M

L

N

G

A

P

D

D

K

L

S

V

R

A

Q

R

A

S

L

L

D

A

E

E

L

V

G

G

V

I

C

G

F

F

L

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A
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A

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P

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Y

F

H
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H

T

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G

S

F
x
Y

R
|
R

H

H

A

A

M
x
A

P

A

V

V

R

R

Q

R

Q

E

L

I

K

G

T

V

R

P

T

T

L

V

F

F

N

N

V

L

L

L

G

G

P

P

L

L

I

T

N

N

P

P

A

A

H

R

P

P

P

R

L

A

A

G

L

L

I

I

G

G

V

C

Y

A

S

F

P

A

E

D

L

L

V

A

L

E

P

V

I

M

A

A

E

G

T

-

L

-

R

-

V

V

L

F

G

A

Y

A

Q

R

R

R

A

S

-

V

-

L

V

V

H

H

-

G

S

D

G

D

G

G

M

L

D
|
D

E
|
E

V

L

S

T

L

T

H

T

A

T

P

T

T

S

V

T

V

I

A

W

E

R

L

V

N

A

G

G

E

S

I

V

K

D

S

K

Y

L

Q

T

L

F

T

D

A

P

A

A

D

G

F

F

G

G

L

F

T

A

P

R

Y

A

H

Q

Q

L

E

D

Q

Q

L

L

A

A

G

G

active site: V82 (= V277)
binding 2-amino-4,5-dimethoxybenzoic acid: N114 (= N309), A155 (= A350), P156 (= P351), H159 (= H354), Y162 (≠ F357), R163 (= R358), A166 (≠ M361)
binding magnesium ion: S95 (= S290), D227 (= D422), E228 (= E423), E228 (= E423)
binding 1-O-pyrophosphono-5-O-phosphono-alpha-D-ribofuranose: V82 (= V277), G83 (= G278), G85 (= G280), G86 (= G281), N93 (= N288), S95 (= S290), T96 (= T291), K111 (= K306), N114 (= N309), A117 (≠ V312), S118 (= S313), S119 (= S314)

Query Sequence

>BWI76_RS11855 FitnessBrowser__Koxy:BWI76_RS11855
MADILLLDNIDSFTYNLADQLRSNGHNVVIYRNSVPAQALIERIGTMKNPVLMLSPGPGT
PSEAGCMPELLIRMRGKLPIIGICLGHQAIVEAYGGYVGQAGEILHGKASSIEHDGQAMF
AGLSNPLPVARYHSLVGSNIPAGLTINAHFNGMVMAVRHDADRVCGFQFHPESILTTQGA
RLLEQTLAWALQKLEQTNTLQPILEKLYQAETLSQQESHQLFSAVVRGEVKPEQLAAALV
SMKVRGEHPQEIAGAATALLENAAPFPRPDYLFADIVGTGGDGSNSINISTASAFVAAAC
GLKVAKHGNRSVSSKSGSSDLLAAFGINLDMNADKSRQALDELGVCFLFAPKYHTGFRHA
MPVRQQLKTRTLFNVLGPLINPAHPPLALIGVYSPELVLPIAETLRVLGYQRAAVVHSGG
MDEVSLHAPTVVAELNNGEIKSYQLTAADFGLTPYHQEQLAGGTPEENRDILTRLLQGKG
EAAHEAAVAANVAMLMRLHGYEDLKANAQQVIDVLHSGAAYDRVTALAARG

Or try a new SitesBLAST search

SitesBLAST's Database

SitesBLAST's database includes (1) SwissProt entries with experimentally-supported functional features; and (2) protein structures with bound ligands, from the BioLip database.

by Morgan Price, Arkin group
Lawrence Berkeley National Laboratory