SitesBLAST
Comparing BWI76_RS14900 BWI76_RS14900 oxidoreductase to proteins with known functional sites using BLASTp with E ≤ 0.001.
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Found 20 (the maximum) hits to proteins with known functional sites (download)
1wwkA Crystal structure of phosphoglycerate dehydrogenase from pyrococcus horikoshii ot3
33% identity, 92% coverage: 9:314/331 of query aligns to 3:286/304 of 1wwkA
- active site: S96 (≠ N124), R230 (= R258), D254 (= D282), E259 (= E287), H278 (= H306)
- binding nicotinamide-adenine-dinucleotide: V100 (= V128), G146 (= G177), F147 (≠ L178), G148 (= G179), R149 (≠ H180), I150 (= I181), Y168 (= Y199), D169 (= D200), P170 (≠ K201), V201 (≠ A229), P202 (≠ R230), T207 (= T235), T228 (= T256), S229 (≠ A257), D254 (= D282), H278 (= H306), G280 (≠ A308)
3dc2A Crystal structure of serine bound d-3-phosphoglycerate dehydrogenase from mycobacterium tuberculosis (see paper)
40% identity, 76% coverage: 64:314/331 of query aligns to 35:285/526 of 3dc2A
Sites not aligning to the query:
3ddnB Crystal structure of hydroxypyruvic acid phosphate bound d-3- phosphoglycerate dehydrogenase in mycobacterium tuberculosis (see paper)
40% identity, 76% coverage: 64:314/331 of query aligns to 36:286/525 of 3ddnB
Sites not aligning to the query:
6cwaA Crystal structure phgdh in complex with nadh and 3-phosphoglycerate at 1.77 a resolution (see paper)
36% identity, 83% coverage: 43:318/331 of query aligns to 17:289/299 of 6cwaA
- binding 1,4-dihydronicotinamide adenine dinucleotide: N96 (= N124), A100 (≠ V128), R149 (≠ H180), I150 (= I181), Y168 (= Y199), D169 (= D200), P170 (≠ K201), I171 (≠ Y202), H200 (= H228), T201 (≠ A229), P202 (≠ R230), T207 (= T235), C228 (≠ T256), A229 (= A257), R230 (= R258), H277 (= H306), G279 (≠ A308)
6rj5A Crystal structure of phgdh in complex with compound 39 (see paper)
36% identity, 83% coverage: 43:318/331 of query aligns to 18:290/301 of 6rj5A
6rj3A Crystal structure of phgdh in complex with compound 15 (see paper)
36% identity, 83% coverage: 43:318/331 of query aligns to 17:289/297 of 6rj3A
7ewhA Crystal structure of human phgdh in complex with homoharringtonine (see paper)
36% identity, 83% coverage: 43:318/331 of query aligns to 18:290/302 of 7ewhA
- binding (3beta)-O~3~-[(2R)-2,6-dihydroxy-2-(2-methoxy-2-oxoethyl)-6-methylheptanoyl]cephalotaxine: L146 (≠ V176), G147 (= G177), L148 (= L178), G149 (= G179), R150 (≠ H180), I151 (= I181), G152 (≠ A182), D170 (= D200), H201 (= H228), T202 (≠ A229), P203 (≠ R230)
6rj2A Crystal structure of phgdh in complex with compound 40 (see paper)
36% identity, 83% coverage: 43:318/331 of query aligns to 15:287/299 of 6rj2A
- binding ~{N}-[(1~{R})-1-[4-(ethanoylsulfamoyl)phenyl]ethyl]-2-methyl-5-phenyl-pyrazole-3-carboxamide: G146 (= G179), I148 (= I181), Y166 (= Y199), D167 (= D200), P168 (≠ K201), I169 (≠ Y202), I170 (≠ V203), H198 (= H228), T199 (≠ A229), L208 (= L238), R228 (= R258)
6rihA Crystal structure of phgdh in complex with compound 9 (see paper)
36% identity, 83% coverage: 43:318/331 of query aligns to 18:290/302 of 6rihA
7dkmA Phgdh covalently linked to oridonin (see paper)
36% identity, 83% coverage: 43:318/331 of query aligns to 19:291/306 of 7dkmA
- binding nicotinamide-adenine-dinucleotide: T74 (≠ G100), A102 (≠ V128), G148 (= G177), R151 (≠ H180), I152 (= I181), Y170 (= Y199), D171 (= D200), P172 (≠ K201), I173 (≠ Y202), H202 (= H228), T203 (≠ A229), P204 (≠ R230), T209 (= T235), C230 (≠ T256), A231 (= A257), R232 (= R258), H279 (= H306), G281 (≠ A308)
Sites not aligning to the query:
- binding (1beta,6beta,7beta,8alpha,9beta,10alpha,13alpha,14R,16beta)-1,6,7,14-tetrahydroxy-7,20-epoxykauran-15-one: 14, 17, 18, 293
6plgA Crystal structure of human phgdh complexed with compound 15 (see paper)
36% identity, 83% coverage: 43:318/331 of query aligns to 18:290/303 of 6plgA
6plfA Crystal structure of human phgdh complexed with compound 1 (see paper)
36% identity, 83% coverage: 43:318/331 of query aligns to 19:291/305 of 6plfA
O43175 D-3-phosphoglycerate dehydrogenase; 3-PGDH; 2-oxoglutarate reductase; Malate dehydrogenase; EC 1.1.1.95; EC 1.1.1.399; EC 1.1.1.37 from Homo sapiens (Human) (see 3 papers)
36% identity, 76% coverage: 69:318/331 of query aligns to 46:295/533 of O43175
- T78 (≠ G100) binding
- R135 (≠ K157) to W: in PHGDHD; results in a 2-fold decrease in enzyme activity with 3-phosphohydroxypyruvate, but no change in substrate affinity; dbSNP:rs267606949
- RI 155:156 (≠ HI 180:181) binding
- D175 (= D200) binding
- T207 (≠ A229) binding
- CAR 234:236 (≠ TAR 256:258) binding
- D260 (= D282) binding
- V261 (≠ T283) to M: in PHGDHD; results in a four-fold decrease in substrate affinity and a slight increase in maximal enzyme activity with 3-phosphohydroxypyruvate; dbSNP:rs267606947
- HLGA 283:286 (≠ HIAG 306:309) binding
Sites not aligning to the query:
- 1 modified: Initiator methionine, Removed
- 2 modified: N-acetylalanine
- 373 A → T: in PHGDHD; results in almost undetectable enzyme activity with 3-phosphohydroxypyruvate; dbSNP:rs201553627
- 377 G → S: in PHGDHD; results in a 2-fold decrease in enzyme activity with 3-phosphohydroxypyruvate, but no change in substrate affinity; dbSNP:rs267606948
- 425 V → M: in PHGDHD; results in almost undetectable enzyme activity with 3-phosphohydroxypyruvate; dbSNP:rs121907988
- 490 V → M: in PHGDHD; results in almost undetectable enzyme activity with 3-phosphohydroxypyruvate; dbSNP:rs121907987
6plfB Crystal structure of human phgdh complexed with compound 1 (see paper)
36% identity, 76% coverage: 69:318/331 of query aligns to 36:281/292 of 6plfB
- binding 4-{(1S)-1-[(5-chloro-6-{[(5S)-2-oxo-1,3-oxazolidin-5-yl]methoxy}-1H-indole-2-carbonyl)amino]-2-hydroxyethyl}benzoic acid: R141 (≠ H180), Y160 (= Y199), D161 (= D200), P162 (≠ K201), I164 (≠ V203), L179 (= L215), T193 (≠ A229), P194 (≠ R230), S198 (≠ E234), L202 (= L238)
5aovA Ternary crystal structure of pyrococcus furiosus glyoxylate hydroxypyruvate reductase in presence of glyoxylate (see paper)
33% identity, 76% coverage: 64:314/331 of query aligns to 39:296/334 of 5aovA
- active site: L100 (≠ N124), R241 (= R258), D265 (= D282), E270 (= E287), H288 (= H306)
- binding glyoxylic acid: M52 (≠ Q77), L53 (≠ F78), L53 (≠ F78), Y74 (≠ L98), A75 (≠ R99), V76 (≠ G100), G77 (= G101), R241 (= R258), H288 (= H306)
- binding nadp nicotinamide-adenine-dinucleotide phosphate: V76 (≠ G100), T104 (≠ V128), F158 (≠ L178), G159 (= G179), R160 (≠ H180), I161 (= I181), S180 (≠ D200), R181 (≠ K201), A211 (≠ H228), V212 (≠ A229), P213 (≠ R230), T218 (= T235), I239 (≠ T256), A240 (= A257), R241 (= R258), H288 (= H306), G290 (≠ A308)
P87228 Putative D-3-phosphoglycerate dehydrogenase; 3-PGDH; 2-oxoglutarate reductase; EC 1.1.1.95; EC 1.1.1.399 from Schizosaccharomyces pombe (strain 972 / ATCC 24843) (Fission yeast) (see paper)
30% identity, 95% coverage: 18:330/331 of query aligns to 67:368/466 of P87228
- S87 (≠ Q44) modified: Phosphoserine
- S258 (≠ T232) modified: Phosphoserine
1dxyA Structure of d-2-hydroxyisocaproate dehydrogenase (see paper)
31% identity, 73% coverage: 76:317/331 of query aligns to 51:306/330 of 1dxyA
- active site: S101 (≠ N124), R234 (= R258), D258 (= D282), E263 (= E287), H295 (= H306)
- binding 2-oxo-4-methylpentanoic acid: V77 (≠ G100), Y100 (≠ R123), Y298 (≠ G309)
- binding nicotinamide-adenine-dinucleotide: Y100 (≠ R123), G152 (= G177), G154 (= G179), H155 (= H180), I156 (= I181), Y174 (= Y199), D175 (= D200), P176 (≠ K201), H204 (= H228), V205 (≠ A229), P206 (≠ R230), N211 (≠ T235), T232 (= T256), A233 (= A257), R234 (= R258), H295 (= H306), Y298 (≠ G309)
P17584 D-2-hydroxyisocaproate dehydrogenase; D-HICDH; EC 1.1.1.- from Lacticaseibacillus paracasei (Lactobacillus paracasei) (see paper)
31% identity, 73% coverage: 76:317/331 of query aligns to 51:306/333 of P17584
6biiA Crystal structure of pyrococcus yayanosii glyoxylate hydroxypyruvate reductase in complex with NADP and malonate (re-refinement of 5aow) (see paper)
32% identity, 80% coverage: 64:327/331 of query aligns to 38:308/332 of 6biiA
- active site: L99 (≠ N124), R240 (= R258), D264 (= D282), E269 (= E287), H287 (= H306)
- binding nadp nicotinamide-adenine-dinucleotide phosphate: V75 (≠ G100), T103 (≠ V128), G156 (= G177), F157 (≠ L178), G158 (= G179), R159 (≠ H180), I160 (= I181), A179 (≠ D200), R180 (≠ K201), S181 (≠ Y202), K183 (≠ A204), V211 (≠ A229), P212 (≠ R230), E216 (= E234), T217 (= T235), V238 (≠ T256), A239 (= A257), R240 (= R258), D264 (= D282), H287 (= H306), G289 (≠ A308)
7cvpA The crystal structure of human phgdh from biortus.
36% identity, 62% coverage: 114:318/331 of query aligns to 41:244/254 of 7cvpA
- binding nicotinamide-adenine-dinucleotide: G101 (= G177), G103 (= G179), R104 (≠ H180), I105 (= I181), Y123 (= Y199), D124 (= D200), P125 (≠ K201), I126 (≠ Y202), H155 (= H228), T156 (≠ A229), P157 (≠ R230), T162 (= T235), C183 (≠ T256), A184 (= A257), R185 (= R258), H232 (= H306), G234 (≠ A308)
Query Sequence
>BWI76_RS14900 BWI76_RS14900 oxidoreductase
MKCLAIADLFINAAMMDAGLNALKDKGIAVEVREWSHESIEKLQEDNLLVEQQGSEALAL
PAALLQGAEETEILIVQFAPVNAAVFDKLPKLKYVGVLRGGIENVNQAEAKARGIEVMNT
PGRNARSVAEFTVGMILAEMRNIARSHDALRDKFWRKESPNHRAIPELGGKVVGLVGLGH
IAQLVAGFLRGFGSEIIFYDKYVAGHDSYEKVDSLDELVRRADVISLHARLTAETENLIN
AHHFDLMKESAIIVNTARSGLINEADLIAALRAGKIMGAALDTFDDEPLPDDSAFYSLNN
VTITPHIAGSTIDAFSNSPKLFSEILLKKIG
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SitesBLAST's Database
SitesBLAST's database includes
(1) SwissProt
entries with experimentally-supported functional features;
and (2) protein structures with bound ligands, from the
BioLip database.
by Morgan Price,
Arkin group
Lawrence Berkeley National Laboratory