Comparing BWI76_RS15105 FitnessBrowser__Koxy:BWI76_RS15105 to proteins with known functional sites using BLASTp with E ≤ 0.001.
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Found 11 hits to proteins with known functional sites (download)
6qyiB Structure of hpab from e.Coli in complex with fad (see paper)
53% identity, 96% coverage: 10:494/504 of query aligns to 15:501/520 of 6qyiB
6eb0C Structure of 4-hydroxyphenylacetate 3-monooxygenase (hpab), oxygenase component from escherichia coli (see paper)
53% identity, 96% coverage: 10:494/504 of query aligns to 14:500/518 of 6eb0C
6b1bA Structure of 4-hydroxyphenylacetate 3-monooxygenase (hpab), oxygenase component from escherichia coli mutant xs6 (apo enzyme) (see paper)
53% identity, 96% coverage: 10:494/504 of query aligns to 14:491/510 of 6b1bA
Sites not aligning to the query:
6qyhB Structure of apo hpab from e.Coli (see paper)
53% identity, 96% coverage: 10:494/504 of query aligns to 15:493/512 of 6qyhB
Q5SJP8 4-hydroxyphenylacetate 3-monooxygenase oxygenase component; 4-HPA 3-hydroxylase; 4-HPA 3-monooxygenase large component; EC 1.14.14.9 from Thermus thermophilus (strain ATCC 27634 / DSM 579 / HB8) (see paper)
33% identity, 89% coverage: 10:456/504 of query aligns to 4:443/481 of Q5SJP8
Sites not aligning to the query:
2yyjA Crystal structure of the oxygenase component (hpab) of 4- hydroxyphenylacetate 3-monooxygenase complexed with fad and 4- hydroxyphenylacetate (see paper)
33% identity, 89% coverage: 10:456/504 of query aligns to 3:442/476 of 2yyjA
Sites not aligning to the query:
O87009 FADH(2)-dependent monooxygenase TftD; Chlorophenol-4-monooxygenase component 2; Two component enzyme D; EC 1.14.14.- from Burkholderia cepacia (Pseudomonas cepacia) (see paper)
27% identity, 89% coverage: 8:457/504 of query aligns to 1:448/515 of O87009
7e8pD Crystal structure of a flavin-dependent monooxygenase hada wild type complexed with reduced fad and 4-nitrophenol (see paper)
26% identity, 95% coverage: 10:488/504 of query aligns to 4:481/482 of 7e8pD
6vjrA Oxygen tolerant archeal 4hydroxybutyrylcoa dehydratase (4hbd) from n. Maritimus
25% identity, 85% coverage: 7:435/504 of query aligns to 1:428/502 of 6vjrA
Sites not aligning to the query:
P55792 4-hydroxybutyryl-CoA dehydratase/vinylacetyl-CoA-Delta-isomerase; 4-BUDH; Gamma-aminobutyrate metabolism dehydratase/isomerase; EC 4.2.1.120; EC 5.3.3.3 from Clostridium aminobutyricum (see paper)
23% identity, 67% coverage: 24:359/504 of query aligns to 19:357/490 of P55792
Sites not aligning to the query:
1u8vA Crystal structure of 4-hydroxybutyryl-coa dehydratase from clostridium aminobutyricum: radical catalysis involving a [4fe-4s] cluster and flavin (see paper)
22% identity, 67% coverage: 24:359/504 of query aligns to 19:357/490 of 1u8vA
Sites not aligning to the query:
>BWI76_RS15105 FitnessBrowser__Koxy:BWI76_RS15105
MGNKSVSLPTGNDFLAGLNDGREIWAYGEKVDNVITHPAFRNSALSIAKLYDALHDEKYQ
AILTTPCDTGSGGVTHPFFRVPHSVDDLIRDRDAIAQWARLSYGWLGRSPDFKASFLTTL
GANPEAYGEYADNARHWYKRSQEELLYWNHAIINPPIDRNLPPDQIEDICIHVEKECDDG
VIVSGAKVVATGSALTHYNFIAHYGLPVKKREFALVCTLPMNAPGLKLVCRPSYSLAAEK
IGSPFDYPLSSRYDENDMIFILDKVKIPWENIFIYGDTAKAATFLPSSGFLHRSTFHGVT
RLAVKIDFICGLFIKGVEATGVADFRGIQTRVGEMLAWRNLFWAISDAMAKSPNPWHNGA
LLPNLDYGLAYRWFMSLGYPRIREIIEQDLGSALIYINSHAKDFAAPELEPYLKKYMRGS
NGMDAVERVKLMKLIWDSVGTEFASRHELYERNYTGSHENVRIELLLAAQMSGQVQEYKG
FAEQCMMDYDLDGWLDRDYHWPVD
Or try a new SitesBLAST search
SitesBLAST's database includes (1) SwissProt entries with experimentally-supported functional features; and (2) protein structures with bound ligands, from the BioLip database.
Lawrence Berkeley National Laboratory