SitesBLAST
Comparing BWI76_RS15930 FitnessBrowser__Koxy:BWI76_RS15930 to proteins with known functional sites using BLASTp with E ≤ 0.001.
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Found 20 (the maximum) hits to proteins with known functional sites (download)
P18776 Anaerobic dimethyl sulfoxide reductase chain B; DMSO reductase iron-sulfur subunit from Escherichia coli (strain K12) (see 2 papers)
91% identity, 100% coverage: 1:205/205 of query aligns to 1:205/205 of P18776
- M1 (= M1) modified: Initiator methionine, Removed
- C102 (= C102) mutation C->F,S,W,Y: Loss of electron transfer from menaquinol to DMSO.
1kqfB Formate dehydrogenase n from e. Coli (see paper)
34% identity, 80% coverage: 3:167/205 of query aligns to 27:200/289 of 1kqfB
- binding protoporphyrin ix containing fe: Y137 (= Y104)
- binding iron/sulfur cluster: K31 (≠ F7), C38 (= C14), I39 (≠ T15), G40 (= G16), C41 (= C17), K42 (= K18), C44 (= C20), C48 (= C24), N52 (≠ K28), T77 (≠ Q48), M79 (vs. gap), C99 (= C67), M100 (≠ N68), H101 (= H69), C102 (= C70), P105 (= P73), C107 (= C75), C111 (= C79), P112 (= P80), I117 (≠ M84), V125 (= V92), C132 (= C99), I133 (= I100), G134 (= G101), C135 (= C102), G136 (≠ R103), Y137 (= Y104), C138 (= C105), C142 (= C109), I146 (≠ A113), P147 (= P114), V156 (≠ M123), K158 (= K125), C159 (= C126), L161 (≠ G128), C162 (= C129), P172 (= P139), C174 (= C141), C178 (= C145), P179 (= P146), I183 (≠ L150)
Sites not aligning to the query:
P0AAJ3 Formate dehydrogenase, nitrate-inducible, iron-sulfur subunit; Anaerobic formate dehydrogenase iron-sulfur subunit; Formate dehydrogenase-N subunit beta; FDH-N subunit beta from Escherichia coli (strain K12) (see paper)
34% identity, 80% coverage: 3:167/205 of query aligns to 28:201/294 of P0AAJ3
- C39 (= C14) binding
- C42 (= C17) binding
- C45 (= C20) binding
- C49 (= C24) binding
- C100 (= C67) binding
- C103 (= C70) binding
- C108 (= C75) binding
- C112 (= C79) binding
- C133 (= C99) binding
- C136 (= C102) binding
- C139 (= C105) binding
- C143 (= C109) binding
- C160 (= C126) binding
- C163 (= C129) binding
- C175 (= C141) binding
- C179 (= C145) binding
2vpyF Polysulfide reductase with bound quinone inhibitor, pentachlorophenol (pcp) (see paper)
37% identity, 75% coverage: 4:157/205 of query aligns to 3:147/193 of 2vpyF
- binding pentachlorophenol: I91 (= I100), C93 (= C102)
- binding iron/sulfur cluster: C13 (= C14), G15 (= G16), C16 (= C17), C19 (= C20), C23 (= C24), N27 (≠ K28), L36 (≠ H37), I38 (= I39), P55 (≠ S64), Q57 (≠ A66), C58 (= C67), L59 (≠ N68), C61 (= C70), P65 (≠ A74), C66 (= C75), C70 (= C79), P71 (= P80), V83 (= V92), C90 (= C99), I91 (= I100), A92 (≠ G101), C93 (= C102), G94 (≠ R103), C96 (= C105), C100 (= C109), P101 (= P110), Y102 (= Y111), R105 (≠ Q115), V113 (≠ M123), C116 (= C126), F118 (≠ G128), C119 (= C129), P129 (= P139), A130 (≠ I140), C131 (= C141), C135 (= C145), C139 (≠ A149), R140 (≠ L150)
Sites not aligning to the query:
2vpwF Polysulfide reductase with bound menaquinone (see paper)
37% identity, 75% coverage: 4:157/205 of query aligns to 3:147/193 of 2vpwF
- binding iron/sulfur cluster: C13 (= C14), G15 (= G16), C16 (= C17), C19 (= C20), C23 (= C24), N27 (≠ K28), L36 (≠ H37), I38 (= I39), P55 (≠ S64), Q57 (≠ A66), C58 (= C67), L59 (≠ N68), H60 (= H69), C61 (= C70), P65 (≠ A74), C66 (= C75), C70 (= C79), P71 (= P80), V83 (= V92), C90 (= C99), I91 (= I100), A92 (≠ G101), C93 (= C102), G94 (≠ R103), C96 (= C105), C100 (= C109), P101 (= P110), Y102 (= Y111), R105 (≠ Q115), V113 (≠ M123), C116 (= C126), F118 (≠ G128), C119 (= C129), P129 (= P139), A130 (≠ I140), C131 (= C141), C135 (= C145), C139 (≠ A149), R140 (≠ L150)
Sites not aligning to the query:
2ivfB Ethylbenzene dehydrogenase from aromatoleum aromaticum (see paper)
35% identity, 82% coverage: 27:194/205 of query aligns to 89:268/337 of 2ivfB
- binding fe3-s4 cluster: C148 (= C79), T150 (≠ S81), I153 (≠ M84), C169 (= C99), K170 (≠ I100), G171 (= G101), H172 (≠ C102), R173 (= R103), H174 (≠ Y104), C175 (= C105), S193 (≠ M123)
- binding protoporphyrin ix containing fe: T150 (≠ S81), K170 (≠ I100), H172 (≠ C102)
- binding iron/sulfur cluster: M135 (≠ A66), C136 (= C67), N137 (= N68), H138 (= H69), C139 (= C70), P142 (= P73), C144 (= C75), V162 (= V92), C179 (= C109), A183 (= A113), I184 (≠ P114), K195 (= K125), C196 (= C126), I197 (≠ D127), L198 (≠ G128), C199 (= C129), N209 (≠ P139), C211 (= C141), C215 (= C145), V219 (≠ A149), R220 (≠ L150)
Sites not aligning to the query:
- binding iron/sulfur cluster: 13, 14, 15, 16, 17, 19, 23, 27, 38, 39, 41
6cz7B The arsenate respiratory reductase (arr) complex from shewanella sp. Ana-3 (see paper)
28% identity, 92% coverage: 4:191/205 of query aligns to 2:220/234 of 6cz7B
- binding iron/sulfur cluster: C12 (= C14), V13 (≠ T15), G14 (= G16), C15 (= C17), G16 (≠ K18), C18 (= C20), C22 (= C24), N26 (≠ K28), Y52 (= Y62), P54 (≠ S64), C57 (= C67), N58 (= N68), H59 (= H69), C60 (= C70), A63 (≠ P73), P64 (≠ A74), C65 (= C75), C69 (= C79), P70 (= P80), T82 (≠ V92), C89 (= C99), I90 (= I100), G91 (= G101), C92 (= C102), K93 (≠ R103), C95 (= C105), C99 (= C109), Y101 (= Y111), V103 (≠ A113), I104 (≠ P114), T161 (≠ M123), K163 (= K125), C164 (= C126), F166 (≠ G128), C167 (= C129), C179 (= C141), C183 (= C145), P184 (= P146), R188 (≠ L150)
Q7WTT9 Arsenate respiratory reductase iron-sulfur subunit ArrB; Arsenate respiratory reductase small subunit; ARR small subunit from Shewanella sp. (strain ANA-3) (see paper)
28% identity, 92% coverage: 4:191/205 of query aligns to 2:220/234 of Q7WTT9
- C12 (= C14) binding
- C15 (= C17) binding
- C18 (= C20) binding
- C22 (= C24) binding
- C57 (= C67) binding
- C60 (= C70) binding
- C65 (= C75) binding
- C69 (= C79) binding
- C89 (= C99) binding
- C92 (= C102) binding
- C95 (= C105) binding
- C99 (= C109) binding
- C164 (= C126) binding
- C167 (= C129) binding
- C179 (= C141) binding
- C183 (= C145) binding
5ch7B Crystal structure of the perchlorate reductase pcrab - phe164 gate switch intermediate - from azospira suillum ps (see paper)
26% identity, 97% coverage: 4:201/205 of query aligns to 7:280/329 of 5ch7B
- binding fe3-s4 cluster: C145 (= C79), I150 (≠ M84), C166 (= C99), K167 (≠ I100), G168 (= G101), A169 (≠ C102), Q170 (≠ R103), A171 (≠ Y104), C172 (= C105), A190 (≠ M123)
- binding iron/sulfur cluster: C17 (= C14), I18 (≠ T15), G19 (= G16), C20 (= C17), Q21 (≠ K18), C23 (= C20), C27 (= C24), W31 (vs. gap), R43 (≠ K28), V45 (≠ L30), P130 (≠ S64), C133 (= C67), N134 (= N68), C136 (= C70), P139 (= P73), C141 (= C75), C176 (= C109), C193 (= C126), G195 (= G128), C196 (= C129), C208 (= C141), C212 (= C145), V213 (≠ P146), G214 (≠ L147)
3egwB The crystal structure of the narghi mutant narh - c16a
40% identity, 48% coverage: 56:153/205 of query aligns to 173:271/509 of 3egwB
- binding fe3-s4 cluster: P181 (≠ S64), C196 (= C79), S198 (= S81), I201 (≠ M84), C217 (= C99), R218 (≠ I100), G219 (= G101), W220 (≠ C102), R221 (= R103), C223 (= C105), S241 (≠ M123), C263 (= C145), G265 (≠ L147), R268 (≠ L150)
- binding protoporphyrin ix containing fe: W220 (≠ C102), R221 (= R103)
- binding iron/sulfur cluster: C184 (= C67), E185 (≠ N68), H186 (= H69), C187 (= C70), P190 (= P73), C192 (= C75), C227 (= C109), I232 (≠ P114), K243 (= K125), C244 (= C126), F246 (≠ G128), C247 (= C129), T257 (≠ P139), C259 (= C141)
Sites not aligning to the query:
P11349 Respiratory nitrate reductase 1 beta chain; Nitrate reductase A subunit beta; Quinol-nitrate oxidoreductase subunit beta; EC 1.7.5.1 from Escherichia coli (strain K12) (see 2 papers)
40% identity, 48% coverage: 56:153/205 of query aligns to 173:271/512 of P11349
- C184 (= C67) binding
- C187 (= C70) binding
- C192 (= C75) binding
- C196 (= C79) binding
- C217 (= C99) binding
- C223 (= C105) binding
- C227 (= C109) binding
- C244 (= C126) binding
- C247 (= C129) binding
- C259 (= C141) binding
- C263 (= C145) binding
Sites not aligning to the query:
- 16 binding
- 19 binding
- 22 binding
- 26 binding
1h0hB Tungsten containing formate dehydrogenase from desulfovibrio gigas (see paper)
31% identity, 75% coverage: 6:159/205 of query aligns to 3:170/214 of 1h0hB
- binding iron/sulfur cluster: F4 (= F7), C11 (= C14), T12 (= T15), A13 (≠ G16), C14 (= C17), R15 (≠ K18), C17 (= C20), C21 (= C24), H25 (vs. gap), K50 (≠ E41), Q71 (≠ A66), C72 (= C67), R73 (≠ N68), C75 (= C70), P79 (≠ A74), C80 (= C75), V101 (= V92), C120 (= C109), P125 (= P114), K136 (= K125), C137 (= C126), D138 (= D127), M139 (≠ G128), C140 (= C129), C152 (= C141), C156 (= C145), P157 (= P146), A160 (= A149)
Q8GC87 Formate dehydrogenase subunit beta; FDH subunit beta; Formate dehydrogenase small subunit from Megalodesulfovibrio gigas (strain ATCC 19364 / DSM 1382 / NCIMB 9332 / VKM B-1759) (Desulfovibrio gigas) (see paper)
31% identity, 75% coverage: 6:159/205 of query aligns to 4:171/215 of Q8GC87
- C12 (= C14) binding
- C15 (= C17) binding
- C18 (= C20) binding
- C22 (= C24) binding
- C73 (= C67) binding
- C76 (= C70) binding
- C81 (= C75) binding
- C121 (= C109) binding
- C138 (= C126) binding
- C141 (= C129) binding
- C153 (= C141) binding
- C157 (= C145) binding
8cm6D W-formate dehydrogenase c872a from desulfovibrio vulgaris - with formamide (see paper)
30% identity, 74% coverage: 7:158/205 of query aligns to 4:169/214 of 8cm6D
- binding formamide: R59 (vs. gap), P62 (vs. gap)
- binding iron/sulfur cluster: F4 (= F7), C11 (= C14), T12 (= T15), A13 (≠ G16), C14 (= C17), R15 (≠ K18), C17 (= C20), C21 (= C24), K25 (= K28), K50 (vs. gap), C73 (= C67), R74 (≠ N68), C76 (= C70), P79 (= P73), P80 (≠ A74), C81 (= C75), V102 (= V93), C120 (= C109), I124 (≠ A113), P125 (= P114), K136 (= K125), C137 (= C126), D138 (= D127), M139 (≠ G128), C140 (= C129), C152 (= C141), C156 (= C145), P157 (= P146), T158 (≠ L147), T160 (≠ A149), M161 (≠ L150)
8bqgB W-formate dehydrogenase from desulfovibrio vulgaris - soaking with formate 1 min (see paper)
30% identity, 74% coverage: 7:158/205 of query aligns to 4:169/214 of 8bqgB
- binding iron/sulfur cluster: F4 (= F7), C11 (= C14), T12 (= T15), A13 (≠ G16), C14 (= C17), R15 (≠ K18), C17 (= C20), C21 (= C24), K25 (= K28), K50 (vs. gap), C73 (= C67), R74 (≠ N68), C76 (= C70), P80 (≠ A74), C81 (= C75), V102 (= V93), C120 (= C109), I124 (≠ A113), P125 (= P114), K136 (= K125), C137 (= C126), M139 (≠ G128), C140 (= C129), C152 (= C141), C156 (= C145), P157 (= P146), T158 (≠ L147), T160 (≠ A149), M161 (≠ L150)
7qv7B Cryo-em structure of hydrogen-dependent co2 reductase. (see paper)
31% identity, 79% coverage: 10:171/205 of query aligns to 10:180/183 of 7qv7B
- binding iron/sulfur cluster: C14 (= C14), G16 (= G16), C17 (= C17), K18 (= K18), A19 (≠ T19), C20 (= C20), C24 (= C24), H28 (= H37), R48 (≠ Y61), C63 (= C67), R64 (≠ N68), H65 (= H69), C66 (= C70), A69 (≠ P73), C71 (= C75), C75 (= C79), A79 (= A83), I80 (≠ M84), C94 (= C99), I95 (= I100), C97 (= C102), C100 (= C105), C104 (= C109), I109 (vs. gap), C139 (= C126), L141 (≠ G128), C142 (= C129), P148 (= P139), C150 (= C141), C154 (= C145), P155 (= P146), L159 (= L150)
6f0kB Alternative complex iii (see paper)
25% identity, 75% coverage: 4:157/205 of query aligns to 723:918/961 of 6f0kB
- binding fe3-s4 cluster: C803 (= C79), V805 (≠ S81), M818 (≠ V94), C823 (= C99), I824 (= I100), G825 (= G101), C829 (= C105), M869 (= M123)
- binding heme c: N821 (≠ E97), R822 (≠ V98), R884 (vs. gap), N887 (vs. gap)
- binding iron/sulfur cluster: C733 (= C14), T734 (= T15), G735 (= G16), C736 (= C17), N737 (≠ K18), A738 (≠ T19), C739 (= C20), C743 (= C24), W765 (= W47), I768 (≠ V58), C791 (= C67), M792 (≠ N68), H793 (= H69), C794 (= C70), P798 (≠ A74), C799 (= C75), N816 (≠ V92), C833 (= C109), C872 (= C126), Y874 (≠ G128), C875 (= C129), A901 (vs. gap), C902 (= C141), C906 (= C145)
Sites not aligning to the query:
7qv7A Cryo-em structure of hydrogen-dependent co2 reductase. (see paper)
28% identity, 81% coverage: 10:175/205 of query aligns to 8:173/174 of 7qv7A
- binding iron/sulfur cluster: C12 (= C14), L13 (≠ T15), G14 (= G16), C15 (= C17), Y16 (≠ K18), C18 (= C20), C22 (= C24), H26 (vs. gap), L37 (≠ F36), C51 (= C67), R52 (≠ N68), C54 (= C70), C59 (= C75), C63 (= C79), C82 (= C99), I83 (= I100), G84 (= G101), C85 (= C102), C88 (= C105), C92 (= C109), I97 (≠ P114), F99 (≠ Y116), C125 (= C126), L127 (≠ G128), C128 (= C129), A136 (≠ I140), C137 (= C141), C141 (= C145)
6x6uB Wor5 from pyrococcus furiosus, taurine-bound (see paper)
33% identity, 68% coverage: 7:145/205 of query aligns to 5:125/167 of 6x6uB
- binding phosphate ion: D10 (≠ A12)
- binding iron/sulfur cluster: C12 (= C14), S13 (≠ T15), C15 (= C17), R16 (≠ K18), C18 (= C20), C22 (= C24), H26 (≠ Y42), R36 (≠ G52), I37 (≠ V53), V39 (= V58), C52 (= C67), V53 (≠ N68), C55 (= C70), P59 (≠ A74), C60 (= C75), C64 (= C79), P65 (= P80), L69 (≠ M84), C85 (= C99), I86 (= I100), T87 (≠ G101), C88 (= C102), G89 (≠ R103), C91 (= C105), C95 (= C109), P100 (= P114), C112 (= C126), D113 (= D127), L114 (≠ G128), C115 (= C129), P119 (= P139), C121 (= C141), C125 (= C145)
Sites not aligning to the query:
8c0zC Cryoem structure of a tungsten-containing aldehyde oxidoreductase from aromatoleum aromaticum (see paper)
30% identity, 72% coverage: 6:152/205 of query aligns to 4:133/158 of 8c0zC
- binding iron/sulfur cluster: C12 (= C14), T13 (= T15), C15 (= C17), C18 (= C20), C22 (= C24), C53 (= C67), C56 (= C70), C61 (= C75), C65 (= C79), I70 (≠ M84), C86 (= C99), C89 (= C102), C92 (= C105), C96 (= C109), T100 (≠ A113), K112 (= K125), C113 (= C126), C116 (= C129), C122 (= C141), C126 (= C145), T128 (≠ L147)
Query Sequence
>BWI76_RS15930 FitnessBrowser__Koxy:BWI76_RS15930
MSAQYGFFIDSARCTGCKTCELACKDYKNLTPDVSFHRIYEYAGGDWQEDNGVWNQNVFA
YYLSIACNHCEDPACTKVCPSGAMHKRDDGFVVVNEEVCIGCRYCHMACPYGAPQYNAAK
GHMTKCDGCYDRVADGKKPICVESCPLRALDFGPIEELREKHGQLAAVAPLPSAHFTKPS
IVIKPNANSRPTGDTTGYLANPKEV
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SitesBLAST's Database
SitesBLAST's database includes
(1) SwissProt
entries with experimentally-supported functional features;
and (2) protein structures with bound ligands, from the
BioLip database.
by Morgan Price,
Arkin group
Lawrence Berkeley National Laboratory