SitesBLAST

SitesBLAST – Find functional sites

SitesBLAST

Comparing BWI76_RS16140 FitnessBrowser__Koxy:BWI76_RS16140 to proteins with known functional sites using BLASTp with E ≤ 0.001.

Or try Sites on a Tree, PaperBLAST, Conserved Domains, or compare to all protein structures

Found 7 hits to proteins with known functional sites (download)

Q29551 Succinyl-CoA:3-ketoacid coenzyme A transferase 1, mitochondrial; SCOT; 3-oxoacid CoA-transferase 1; Somatic-type succinyl-CoA:3-oxoacid CoA-transferase; SCOT-s; Succinate-coenzyme A transferase; Succinyl-CoA:3-oxoacid CoA transferase; EC 2.8.3.5 from Sus scrofa (Pig) (see 3 papers)
45% identity, 92% coverage: 6:205/218 of query aligns to 302:502/520 of Q29551

query
sites
Q29551

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E344 (= E48) active site, 5-glutamyl coenzyme A thioester intermediate

Sites not aligning to the query:

1:39 modified: transit peptide, Mitochondrion
67 C→A: Decreases KM for acetoacetyl-CoA 3-fold. No effect on KM for acetoacetate, succinyl-CoA and succinate.; C→S: Decreases KM for acetoacetate and acetoacetyl-CoA 2-fold. Increases KM for succinate 2-fold. Decreases KM for succinyl-CoA nearly 4-fold. Strongly reduces catalytic activity.
235 C→S: Similar specific activity to wild-type.

1o9lA Succinate:coenzyme-a transferase (pig heart)
43% identity, 94% coverage: 1:205/218 of query aligns to 245:450/468 of 1o9lA

query
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1o9lA

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Sites not aligning to the query:

binding 2-(ethylmercuri-thio)-benzoic acid: 87, 94, 95, 96, 131, 132, 145

3k6mC Dynamic domains of succinyl-coa:3-ketoacid-coenzyme a transferase from pig heart. (see paper)
44% identity, 92% coverage: 6:205/218 of query aligns to 249:449/466 of 3k6mC

query
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3k6mC

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binding glycerol: I270 (= I27), E291 (= E48), A313 (= A70), V377 (≠ A135), A380 (= A138), T382 (vs. gap), C412 (= C169), N414 (≠ S171)

Sites not aligning to the query:

binding glycerol: 25, 52, 76, 99

3oxoE Succinyl-coa:3-ketoacid coa transferase from pig heart covalently bound to coa (see paper)
43% identity, 94% coverage: 1:205/218 of query aligns to 240:445/462 of 3oxoE

query
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3oxoE

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binding coenzyme a: I266 (= I27), G267 (= G28), I268 (≠ L29), E287 (= E48), G343 (= G104), M345 (≠ Y106), N355 (= N116), I358 (≠ T119), M366 (≠ V128), A369 (= A131), K389 (≠ R150)

8i3yD Crystal structure of asct from trypanosoma brucei in complex with succinyl-coa.
42% identity, 94% coverage: 6:209/218 of query aligns to 257:459/471 of 8i3yD

query
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8i3yD

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Sites not aligning to the query:

binding calcium ion: 201, 229

8i40A Crystal structure of asct from trypanosoma brucei in complex with coa.
42% identity, 94% coverage: 6:209/218 of query aligns to 253:455/467 of 8i40A

query
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8i40A

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E

E

I

I

F

A

N

E

G

D

L

T

S

T

F

V

E

D

E

Q

L

V

Q

K

R

K

I

L

T

T

P

G

V

V

A

G

L

F

binding coenzyme a: I274 (= I27), I276 (≠ L29), E295 (= E48), G351 (= G104), L353 (≠ Y106), N363 (= N116), G376 (= G130), K401 (= K155)

8i3yA Crystal structure of asct from trypanosoma brucei in complex with succinyl-coa.
42% identity, 92% coverage: 9:209/218 of query aligns to 250:449/459 of 8i3yA

query
sites
8i3yA

M

I

A

A

Q

R

R

R

V

A

A

A

R

L

D

E

I

F

P

A

E

N

G

G

A

M

Y

Y

V

V

N

N

L

L

R
x
G

I
|
I

G
|
G

L

I

P

P

T

T

R

E

I

S

A

S

N

N

Y

Y

L

I

P

P

A

A

D

G

K

V

E

N

I

V

F

V

L

L

H

Q

S

S

E
|
E

N

N

G

G

L

L

L

I

G

G

M

M

G

G

P

P

K

F

P

P

L

T

P

E

G

D

E

K

E

V

D

D

P

A

E

D

L

W

I

I

N

N

A
|
A

G

G

K
|
K

E

Q

Y

T

V

I

T

S

L

H

L

L

E

A

G

G

G

S

C

A

Y

L

F

F

H

D

H

S

G

A

D

T

S

S

F

F

A

A

M

M

M

I

R

R

G

G

H

H

L

M

D

D

I

L

C

T

V

M

L

L

G

G

A

A

Y

L

Q

E

V

V

S

A

A

A

S

N

G

G

D

D

L

L

A

A

N
|
N

W

F

S

M

T

I

G

P

A

G

P

K

D

-

A

L

I

V

P

K

A

G

V

P

G
|
G

A
|
A

M

M

D

D

L

L

A

V

I

S

G

C

A

G

R

T

Q

R

V

V

F

V

V

V

M

T

D

T

H

H

L

C

T
x
N

R
x
K

D

N

G

G

E

D

C

P

K
|
K

L

I

V

V

E

E

H

R

C

C

T

R

Y

L

P

P

L

V

T

T

G

G

V

K

G

H

C

C

V

V

S

C

R

R

I

I

Y

I

T

T

D

E

L

Y

A

A

V

V

I

F

D

D

I

V

S

V

E

D

S

G

G

R

P

L

V

V

V

L

R

K

E

E

I

I

F

A

N

E

G

D

L

T

S

T

F

V

E

D

E

Q

L

V

Q

K

R

K

I

L

T

T

P

G

V

V

A

G

L

F

binding succinyl-coenzyme a: G267 (≠ R26), I268 (= I27), G269 (= G28), E289 (= E48), A311 (= A70), K313 (= K72), N357 (= N116), G369 (= G129), G370 (= G130), A371 (= A131), N389 (≠ T149), K390 (≠ R150), K395 (= K155)

Sites not aligning to the query:

Query Sequence

>BWI76_RS16140 FitnessBrowser__Koxy:BWI76_RS16140
MQKLTRDDMAQRVARDIPEGAYVNLRIGLPTRIANYLPADKEIFLHSENGLLGMGPKPLP
GEEDPELINAGKEYVTLLEGGCYFHHGDSFAMMRGGHLDICVLGAYQVSASGDLANWSTG
APDAIPAVGGAMDLAIGARQVFVMMDHLTRDGECKLVEHCTYPLTGVGCVSRIYTDLAVI
DISESGPVVREIFNGLSFEELQRITPVALTFAQLAESA

Or try a new SitesBLAST search

SitesBLAST's Database

SitesBLAST's database includes (1) SwissProt entries with experimentally-supported functional features; and (2) protein structures with bound ligands, from the BioLip database.

by Morgan Price, Arkin group
Lawrence Berkeley National Laboratory