SitesBLAST

SitesBLAST – Find functional sites

SitesBLAST

Comparing BWI76_RS16755 FitnessBrowser__Koxy:BWI76_RS16755 to proteins with known functional sites using BLASTp with E ≤ 0.001.

Or try Sites on a Tree, PaperBLAST, Conserved Domains, or compare to all protein structures

Found 20 (the maximum) hits to proteins with known functional sites (download)

Q48436 Diacetyl reductase [(S)-acetoin forming]; Acetoin(diacetyl) reductase; AR; Meso-2,3-butanediol dehydrogenase; EC 1.1.1.304 from Klebsiella pneumoniae (see paper)
93% identity, 100% coverage: 1:256/256 of query aligns to 1:256/256 of Q48436

query
sites
Q48436

M

M

K

K

V

V

A

A

L
|
L

V
|
V

T
|
T

G
|
G

A
|
A

G
|
G

Q
|
Q

G
|
G

I
|
I

G
|
G

K
|
K

A
|
A

I
|
I

A
|
A

L
|
L

R
|
R

L
|
L

V
|
V

K
|
K

D
|
D

G
|
G

F
|
F

A
|
A

V
|
V

A
|
A

I
|
I

A
|
A

D
|
D

Y

Y

N

N

D

D

A

A

T

T

A

A

Q

K

A

A

V

V

A

A

D

S

E

E

I

I

N

N

R

Q

S

A

G

G

R

R

A

A

L

M

A

A

V

V

K

K

V

V

D
|
D

V

V

S

S

Q

D

R

R

D

D

Q

Q

V

V

F

F

A

A

V

V

E

E

Q

Q

A

A

R

R

K

K

G

T

L

L

G

G

F

F

D

D

V

V

I

I

V

V

N

N

A

A

G

G

V

V

A

A

P

P

S

S

T

T

P

P

I

I

E

E

E

S

I

I

R

T

E

P

D

E

V

I

I

V

D

D

K

K

V

V

Y

Y

N

N

I

I

N

N

V

V

K

K

G

G

V

V

I

I

W

W

G

G

I

I

Q

Q

A

A

V

V

E

E

A

A

F

F

K

K

Q

K

E

E

G

G

H

H

G

G

K

K

I

I

N

N

A

A

C

C

S

S

Q

Q

A

A

G

G

H

H

V

V

G

G

N

N

P

P

E

E

L

L

A

A

V

V

Y

Y

S

S

K
|
K

F

F

A

A

V

V

R

R

G

G

L

L

T

T

Q

Q

T

T

A

A

R

R

D

D

L

L

A

A

H

P

L

L

G

G

I

I

T

T

V

V

N

N

G

G

Y

Y

C

C

P

P

G

G

I

I

V

V

K

K

T

T

P

P

M

M

W

W

A

A

E

E

I

I

D

D

R

R

Q

Q

V

V

S

S

E

E

A

A

G

G

K

K

P

P

L

L

G

G

Y

Y

G

G

T

T

Q

A

E

E

F

F

A

A

K

K

R

R

I

I

T

T

L

L

G

G

R

R

L

L

S

S

E

E

P

P

E

E

D

D

V

V

A

A

C

C

V

V

S

S

Y

Y

L

L

A

A

G

S

P

P

D

D

S

S

N

D

Y

Y

M

M

T

T

G

G

Q

Q

S

S

L

L

I

I

D

D

G

G

M

M

V

V

F

F

N

N

6:33 (vs. 6:33, 100% identical) binding
D59 (= D59) binding
K156 (= K156) binding

1gegE Cryatal structure analysis of meso-2,3-butanediol dehydrogenase (see paper)
93% identity, 100% coverage: 1:256/256 of query aligns to 1:256/256 of 1gegE

query
sites
1gegE

M

M

K

K

V

V

A

A

L

L

V

V

T

T

G
|
G

A

A

G
|
G

Q
|
Q

G
|
G

I
|
I

G

G

K

K

A

A

I

I

A

A

L

L

R

R

L

L

V

V

K

K

D

D

G

G

F

F

A

A

V

V

A

A

I

I

A

A

D
|
D

Y
|
Y

N
|
N

D

D

A

A

T

T

A

A

Q

K

A

A

V

V

A

A

D

S

E

E

I

I

N

N

R

Q

S

A

G

G

R

H

A

A

L

V

A

A

V

V

K

K

V
|
V

D
|
D

V
|
V

S

S

Q

D

R
|
R

D
|
D

Q

Q

V

V

F
|
F

A

A

V

V

E

E

Q

Q

A

A

R

R

K

K

G

T

L

L

G

G

F

F

D

D

V

V

I

I

V

V

N

N

N
|
N

A
|
A

G
|
G

V

V

A

A

P

P

S

S

T

T

P

P

I

I

E

E

E

S

I

I

R

T

E

P

D

E

V

I

I

V

D

D

K

K

V

V

Y

Y

N

N

I
|
I

N

N

V

V

K

K

G

G

V

V

I

I

W

W

G

G

I

I

Q

Q

A

A

V

V

E
|
E

A

A

F

F

K

K

Q

K

E

E

G

G

H

H

G

G

K

K

I

I

N

N

A
|
A

C
|
C

S
|
S

Q

Q

A

A

G

G

H

H

V

V

G

G

N

N

P

P

E

E

L

L

A

A

V

V

Y
|
Y

S

S

K
|
K

F

F

A

A

V

V

R

R

G

G

L

L

T

T

Q

Q

T

T

A

A

R

R

D

D

L

L

A

A

H

P

L

L

G

G

I

I

T

T

V

V

N

N

G

G

Y

Y

C

C

P
|
P

G
|
G

I
|
I

V
|
V

K

K

T
|
T

P
|
P

M
|
M

W
|
W

A

A

E

E

I

I

D

D

R

R

Q

Q

V
|
V

S

S

E

E

A

A

G

G

K

K

P

P

L

L

G

G

Y

Y

G

G

T

T

Q

A

E

E

F

F

A

A

K

K

R

R

I

I

T

T

L

L

G

G

R

R

L

L

S

S

E

E

P

P

E

E

D

D

V

V

A

A

C

C

V

V

S

S

Y

Y

L

L

A

A

G

S

P

P

D

D

S

S

N

D

Y

Y

M

M

T

T

G

G

Q

Q

S

S

L

L

I

I

D

D

G

G

M

M

V

V

F

F

N

N

active site: G13 (= G13), S139 (= S139), Y152 (= Y152), K156 (= K156), V197 (= V197)
binding alpha-D-glucopyranose: R63 (= R63), D64 (= D64), F67 (= F67), E123 (= E123)
binding nicotinamide-adenine-dinucleotide: G9 (= G9), G11 (= G11), Q12 (= Q12), G13 (= G13), I14 (= I14), D33 (= D33), Y34 (= Y34), N35 (= N35), V58 (= V58), D59 (= D59), V60 (= V60), N86 (= N86), A87 (= A87), G88 (= G88), I109 (= I109), A137 (= A137), C138 (= C138), S139 (= S139), Y152 (= Y152), K156 (= K156), P182 (= P182), G183 (= G183), I184 (= I184), V185 (= V185), T187 (= T187), P188 (= P188), M189 (= M189), W190 (= W190)

3wyeA Crystal structure of chimeric engineered (2s,3s)-butanediol dehydrogenase complexed with NAD+
73% identity, 100% coverage: 2:256/256 of query aligns to 1:255/255 of 3wyeA

query
sites
3wyeA

K

K

V

V

A

A

L

L

V

V

T

T

G
|
G

A

A

G
|
G

Q
|
Q

G
|
G

I
|
I

G

G

K

K

A

A

I

I

A

A

L

L

R

R

L

L

V

V

K

K

D

D

G

G

F

F

A

A

V

V

A

A

I

I

A

A

D
|
D

Y
|
Y

N

N

D

D

A

A

T

T

A

A

Q

K

A

A

V

V

A

A

D

S

E

E

I

I

N

N

R

Q

S

A

G

G

R

H

A

A

L

V

A

A

V

V

K

K

V
|
V

D
|
D

V
|
V

S

S

Q

D

R

R

D

D

Q

Q

V

V

F

F

A

A

V

V

E

E

Q

Q

A

A

R

R

K

K

G

T

L

L

G

G

F

F

D

D

V

V

I

I

V

V

N

N

N
|
N

A
|
A

G
|
G

V
x
I

A

A

P

Q

S

I

T

K

P

P

I

L

E

L

E

E

I

V

R

T

E

E

D

E

V

D

I

L

D

K

K

Q

V

I

Y

Y

N

S

I
x
V

N

N

V

V

K

F

G

S

V

V

I

F

W

F

G

G

I

I

Q

Q

A

A

V

V

E

E

A

A

F

F

K

K

Q

K

E

E

G

G

H

H

G

G

K

K

I

I

N

N

A
|
A

C
x
A

S
|
S

Q

I

A

A

G

A

H

I

V

Q

G

G

N

F

P

P

E

I

L

L

A

S

V

A

Y
|
Y

S

S

S

T

K
|
K

F

F

A

A

V

V

R

R

G

G

L

L

T

T

Q

Q

T

T

A

A

R

R

D

D

L

L

A

A

H

P

L

L

G

G

I

I

T

T

V

V

N

N

G

G

Y

Y

C

C

P
|
P

G
|
G

I
|
I

V
|
V

K

G

T
|
T

P
x
G

M
|
M

W
|
W

A

E

E

Q

I

I

D

D

R

A

Q

E

V
x
L

S

S

E

K

A

I

A

N

G

G

K

K

P

P

L

I

G

G

Y

E

G

N

T

F

Q

K

E

E

F

Y

A

S

K

S

R

S

I

I

T

A

L

L

G

G

R

R

L

P

S

S

E

V

P

P

E

E

D

D

V

V

A

A

A

G

C

L

V

V

S

S

Y

F

L

L

A

A

G

S

P

P

D

D

S

S

N

D

Y

Y

M

M

T

T

G

G

Q

Q

S

S

L

L

I

I

D

D

G

G

M

M

V

V

F

F

N

N

active site: G12 (= G13), S138 (= S139), Y151 (= Y152), K155 (= K156), L196 (≠ V197)
binding nicotinamide-adenine-dinucleotide: G8 (= G9), G10 (= G11), Q11 (= Q12), G12 (= G13), I13 (= I14), D32 (= D33), Y33 (= Y34), V57 (= V58), D58 (= D59), V59 (= V60), N85 (= N86), A86 (= A87), G87 (= G88), I88 (≠ V89), V108 (≠ I109), A136 (= A137), A137 (≠ C138), S138 (= S139), Y151 (= Y152), K155 (= K156), P181 (= P182), G182 (= G183), I183 (= I184), V184 (= V185), T186 (= T187), G187 (≠ P188), M188 (= M189), W189 (= W190)

Q9ZNN8 L-2,3-butanediol dehydrogenase; L-BDH; (S,S)-butanediol dehydrogenase; Diacetyl reductase [(S)-acetoin forming]; EC 1.1.1.76; EC 1.1.1.304 from Corynebacterium glutamicum (Brevibacterium saccharolyticum) (see paper)
49% identity, 100% coverage: 1:256/256 of query aligns to 1:258/258 of Q9ZNN8

query
sites
Q9ZNN8

M

M

K

S

K

K

V

V

A

A

L

M

V

V

T

T

G

G

A

G

G

A

Q
|
Q

G
|
G

I
|
I

G

G

K

R

A

G

I

I

A

S

L

E

R

K

L

L

V

A

K

A

D

D

G

G

F

F

A

D

V

I

A

A

I

V

A

A

D
|
D

Y

L

N

P

D

Q

A
x
Q

T

E

A

E

Q

Q

A

A

-

A

-

E

V

T

A

I

D

K

E

L

I

I

N

E

R

A

S

A

G

D

G

Q

R

K

A

A

L

V

A

F

V

V

K

G

V

L

D
|
D

V
|
V

S

T

Q

D

R

K

D

A

Q

N

V

F

F

D

A

S

A

A

V

I

E

D

Q

E

A

A

R

A

K

E

G

K

L

L

G

G

F

F

D

D

V

V

I

L

V

V

N

N

N
|
N

A

A

G

G

V

I

A

A

P

Q

S

I

T

K

P

P

I

L

E

L

E

E

I

V

R

T

E

E

D

E

V

D

I

L

D

K

K

Q

V

I

Y

Y

N

S

I

V

N

N

V

V

K

F

G

S

V

V

I

F

W

F

G

G

I

I

Q

Q

A

A

V

S

E

R

A

K

F

F

K

D

Q

E

E

L

G

G

H

V

G

K

G

G

K

K

I

I

N

N

A

A

C

A

S

S

Q
x
I

A

A

G

A

H

I

V

Q

G

G

N
x
F

P

P

E

I

L

L

A

S

V

A

Y
|
Y

S

S

S

T

K
|
K

F

F

A

A

V

V

R

R

G

G

L

L

T

T

Q

Q

T

A

A

A

R

Q

D

E

L

L

A

A

H

P

L

K

G

G

I

H

T

T

V

V

N

N

G

A

Y

Y

C

A

P
|
P

G
|
G

I
|
I

V
|
V

K
x
G

T
|
T

P

G

M

M

W

W

A

E

E

Q

I

I

D

D

R

A

Q

E

V

L

S

S

E

K

A

I

A

N

G

G

K

K

P

P

L

I

G

G

Y

E

G

N

T

F

Q

K

E

E

F

Y

A

S

K

S

R

S

I

I

T

A

L

L

G

G

R

R

L

P

S

S

E

V

P

P

E

E

D

D

V

V

A

A

A

G

C

L

V

V

S

S

Y

F

L

L

A

A

G

S

P

E

D

N

S

S

N

N

Y

Y

M

V

T

T

G

G

Q

Q

S

V

L

M

L

L

I

V

D

D

G

G

M

M

V

L

F

Y

N

N

QGI 12:14 (= QGI 12:14) binding
D33 (= D33) binding
Q37 (≠ A37) binding
DV 61:62 (= DV 59:60) binding
N88 (= N86) binding
I142 (≠ Q140) mutation to Q: Loss of L-BD oxidizing activity, and does not gain the ability to use meso-BD as substrate; when associated with N-148.; mutation to Q: Loss of L-BD oxidizing activity. Does not gain the ability to use meso-BD as substrate.
F148 (≠ N146) mutation to N: Loss of L-BD oxidizing activity, and does not gain the ability to use meso-BD as substrate; when associated with Q-142.; mutation to N: Loss of L-BD oxidizing activity. Does not gain the ability to use meso-BD as substrate.
Y154 (= Y152) binding
K158 (= K156) binding
PGIVGT 184:189 (≠ PGIVKT 182:187) binding

3a28C Crystal structure of l-2,3-butanediol dehydrogenase (see paper)
49% identity, 99% coverage: 3:256/256 of query aligns to 2:257/257 of 3a28C

query
sites
3a28C

K

K

V

V

A

A

L

M

V

V

T

T

G
|
G

A

G

G

A

Q
|
Q

G
|
G

I
|
I

G

G

K

R

A

G

I

I

A

S

L

E

R

K

L

L

V

A

K

A

D

D

G

G

F

F

A

D

V

I

A

A

I

V

A

A

D
|
D

Y
x
L

N

P

D

Q

A
x
Q

T

E

A

E

Q

Q

A

A

-

A

-

E

V

T

A

I

D

K

E

L

I

I

N

E

R

A

S

A

G

D

G

Q

R

K

A

A

L

V

A

F

V

V

K

G

V
x
L

D
|
D

V
|
V

S

T

Q

D

R

K

D

A

Q

N

V

F

F

D

A

S

A

A

V

I

E

D

Q

E

A

A

R

A

K

E

G

K

L

L

G

G

F

F

D

D

V

V

I

L

V

V

N

N

N
|
N

A
|
A

G
|
G

V
x
I

A

A

P

Q

S

I

T

K

P

P

I

L

E

L

E

E

I

V

R

T

E

E

D

E

V

D

I

L

D

K

K

Q

V

I

Y

Y

N

S

I
x
V

N

N

V

V

K

F

G

S

V

V

I

F

W

F

G

G

I

I

Q

Q

A

A

V

S

E

R

A

K

F

F

K

D

Q

E

E

L

G

G

H

V

G

K

G

G

K

K

I

I

N

N

A
|
A

C
x
A

S
|
S

Q

I

A

A

G

A

H

I

V

Q

G

G

N

F

P

P

E

I

L

L

A

S

V

A

Y
|
Y

S

S

S

T

K
|
K

F

F

A

A

V

V

R

R

G

G

L

L

T

T

Q

Q

T

A

A

A

R

Q

D

E

L

L

A

A

H

P

L

K

G

G

I

H

T

T

V

V

N

N

G

A

Y

Y

C

A

P
|
P

G
|
G

I
|
I

V
|
V

K

G

T
|
T

P

G

M
|
M

W
|
W

A

E

E

Q

I

I

D

D

R

A

Q

E

V
x
L

S

S

E

K

A

I

A

N

G

G

K

K

P

P

L

I

G

G

Y

E

G

N

T

F

Q

K

E

E

F

Y

A

S

K

S

R

S

I

I

T

A

L

L

G

G

R

R

L

P

S

S

E

V

P

P

E

E

D

D

V

V

A

A

A

G

C

L

V

V

S

S

Y

F

L

L

A

A

G

S

P

E

D

N

S

S

N

N

Y

Y

M

V

T

T

G

G

Q

Q

S

V

L

M

L

L

I

V

D

D

G

G

M

M

V

L

F

Y

N

N

active site: G12 (= G13), S140 (= S139), Y153 (= Y152), K157 (= K156), L198 (≠ V197)
binding nicotinamide-adenine-dinucleotide: G8 (= G9), Q11 (= Q12), G12 (= G13), I13 (= I14), D32 (= D33), L33 (≠ Y34), Q36 (≠ A37), L59 (≠ V58), D60 (= D59), V61 (= V60), N87 (= N86), A88 (= A87), G89 (= G88), I90 (≠ V89), V110 (≠ I109), A138 (= A137), A139 (≠ C138), S140 (= S139), Y153 (= Y152), K157 (= K156), P183 (= P182), G184 (= G183), I185 (= I184), V186 (= V185), T188 (= T187), M190 (= M189), W191 (= W190)

3ak4A Crystal structure of nadh-dependent quinuclidinone reductase from agrobacterium tumefaciens
42% identity, 97% coverage: 5:253/256 of query aligns to 10:255/258 of 3ak4A

query
sites
3ak4A

A

A

L

I

V

V

T

T

G
|
G

A

G

G
x
S

Q
x
K

G
|
G

I
|
I

G

G

K

A

A

A

I

I

A

A

L

R

R

A

L

L

V

D

K

K

D

A

G

G

F

A

A

T

V

V

A

A

I

I

A

A

D
|
D

Y
x
L

N

D

D

V

A

M

T

A

A

A

Q

Q

A

A

V

V

A

V

D

A

E

G

I

L

N

E

R

N

S

G

G

G

G

-

R

-

A

-

L

F

A

A

V

V

K

E

V
|
V

D
|
D

V
|
V

S

T

Q

K

R

R

D

A

Q

S

V

V

F

D

A

A

V

M

E

Q

Q

K

A

A

R

I

K

D

G

A

L

L

G

G

F

F

D

D

V

L

I

L

V

C

N

A

N
|
N

A
|
A

G
|
G

V
|
V

A

S

P

T

S

M

T

R

P

P

I

A

E

V

E

D

I

I

R

T

E

D

D

E

V

E

I

W

D

D

K

F

V

N

Y

F

N

D

I
x
V

N

N

V

A

K

R

G

G

V

V

I

F

W

L

G

A

I

N

Q

Q

A

I

A

A

V

C

E

R

A

H

F

F

K

L

Q

A

E

S

G

N

H

T

G

K

G

G

K

V

I

I

I

V

N

N

A
x
T

C
x
A

S
|
S

Q

L

A

A

G

A

H

K

V

V

G

G

N

A

P

P

E

L

L
|
L

A

A

V

H

Y
|
Y

S

S

S

A

S

S

K
|
K

F

F

A

A

V

V

R

F

G

G

L

W

T

T

Q

Q

T

A

A

L

A

A

R

R

D

E

L

M

A

A

H

P

L

K

G

N

I

I

T

R

V

V

N

N

G

C

Y

V

C

C

P
|
P

G
|
G

I
x
F

V
|
V

K

K

T
|
T

P

A

M
|
M

-

Q

-

E

-
x
R

-

E

-

I

W

W

-
x
E

A

A

E

E

I

L

D

R

R

G

Q

M

V

T

S

P

E

E

A

A

A

V

G

-

K

-

P

-

L

-

G

-

Y

-

G

-

T

R

Q

A

E

E

F

Y

A

V

K

S

R

L

I

T

T

P

L

L

G

G

R

R

L

I

S

E

E

E

P

P

E

E

D

D

V

V

A

A

A

D

C

V

V

V

S

V

Y

F

L

L

A

A

G

S

P

D

D

A

S

A

N

R

Y

F

M

M

T

T

G

G

Q

Q

S

G

L

I

L

N

I

V

D

T

G

G

M

V

active site: G18 (= G13), S141 (= S139), L151 (= L149), Y154 (= Y152), K158 (= K156), E199 (vs. gap)
binding nicotinamide-adenine-dinucleotide: G14 (= G9), S16 (≠ G11), K17 (≠ Q12), G18 (= G13), I19 (= I14), D38 (= D33), L39 (≠ Y34), V60 (= V58), D61 (= D59), V62 (= V60), N88 (= N86), A89 (= A87), G90 (= G88), V91 (= V89), V111 (≠ I109), T139 (≠ A137), A140 (≠ C138), S141 (= S139), Y154 (= Y152), K158 (= K156), P184 (= P182), G185 (= G183), F186 (≠ I184), V187 (= V185), T189 (= T187), M191 (= M189), R194 (vs. gap)

6pejA Structure of sorbitol dehydrogenase from sinorhizobium meliloti 1021 bound to sorbitol
38% identity, 98% coverage: 3:252/256 of query aligns to 7:253/257 of 6pejA

query
sites
6pejA

K

K

V

S

A

A

L

L

V

I

T

T

G

G

A

S

G

A

Q

R

G
|
G

I

I

G

G

K

R

A

A

I

F

A

A

L

E

R

A

L

Y

V

V

K

R

D

E

G

G

F

A

A

T

V

V

A

A

I

I

A

A

D

D

Y

I

N

D

D

I

A

E

T

R

A

A

Q

R

A

Q

V

A

A

A

D

A

E

E

I

I

N

-

R

-

S

-

G

G

G

P

R

A

A

A

L

Y

A

A

V

V

K

Q

V

M

D

D

V

V

S

T

Q

R

R

Q

D

D

Q

S

V

I

F

D

A

A

V

I

E

A

Q

A

A

T

R

V

K

E

G

H

L

A

G

G

F

L

D

D

V

I

I

L

V

V

N

N

A

A

G

A

V

L

A
x
F

P

D

S

L

T

A

P

P

I

I

E

V

E

E

I

I

R

T

E

R

D

E

V

S

I

Y

D

E

K

K

V

L

Y

F

N

A

I

I

N

N

V

V

K

A

G

G

V

T

I

L

W

F

G

T

I

L

Q

Q

A

A

V

A

E

R

A

Q

F

M

K

I

Q

A

E

Q

G

G

H

R

G

G

K

K

I

I

N

N

A

M

C

A

S
|
S

Q
|
Q

A
|
A

G

G

H

R

V
x
R

G

G

N
x
E

P

A

E

L

L

V

A

A

V

I

Y
|
Y

S

C

S

A

S

T

K

K

F

A

A

A

V

V

R

I

G

S

L

L

T

T

Q

Q

T

S

A

A

A

G

R

L

D

D

L

L

A

I

H

K

L

H

G

R

I

I

T

N

V

V

N

N

G

A

Y

I

C

A

P

P

G
|
G

I
x
V

V

V

K

D

T

G

P

E

M
x
H

W
|
W

A

D

E

G

I

V

D

D

R

A

Q

L

V
x
F

S

A

E

R

A

Y

A

E

G

N

K

R

P

P

L

R

G

G

Y

E

G

K

T

K

Q

R

E

L

F
x
V

A

G

K

E

R

A

I

V

T

P

L

F

G

G

R

R

L

M

S

G

E

T

P

A

E

E

D

D

V

L

A

T

A

G

C

M

V

A

S

I

Y

F

L

L

A

A

G

S

P

A

D

E

S

S

N

D

Y

Y

M

I

T

V

G

S

Q

Q

S

T

L

Y

L

N

I

V

D

D

G

G

active site: G17 (= G13), S140 (= S139), Y153 (= Y152)
binding sorbitol: F91 (≠ A90), S140 (= S139), Q141 (= Q140), A142 (= A141), R145 (≠ V144), E147 (≠ N146), Y153 (= Y152), G184 (= G183), V185 (≠ I184), H190 (≠ M189), W191 (= W190), F198 (≠ V197), V213 (≠ F212)

3osuA Crystal structure of the 3-oxoacyl-acyl carrier protein reductase, fabg, from staphylococcus aureus
37% identity, 99% coverage: 1:253/256 of query aligns to 3:244/246 of 3osuA

query
sites
3osuA

M

M

K

T

K

K

V

S

A

A

L

L

V

V

T

T

G

G

A

A

G

S

Q

R

G
|
G

I

I

G

G

K

R

A

S

I

I

A

A

L

L

R

Q

L

L

V

A

K

E

D

E

G

G

F

Y

A

N

V

V

A

A

I

V

A

-

D

N

Y

Y

-

A

-

G

N

S

D

K

A

E

T

K

A

A

Q

E

A

A

V

V

A

V

D

E

E

E

I

I

N

K

R

A

S

K

G

G

G

V

R

D

A

S

L

F

A

A

V

I

K

Q

V

A

D

N

V

V

S

A

Q

D

R

A

D

D

Q

E

V

V

F

K

A

A

A

M

V

I

E

K

Q

E

A

V

R

V

K

S

G

Q

L

F

G

G

G

S

F

L

D

D

V

V

I

L

V

V

N

N

N
|
N

A

A

G

G

V

I

A

T

P

R

S

D

T

N

P

L

I

L

E

M

E

R

I

M

R

K

E

E

D

Q

V

E

I

W

D

D

K

D

V

V

Y

I

N

D

I

T

N

N

V

L

K

K

G

G

V

V

I

F

W

N

G

C

I

I

Q

Q

A

K

A

A

V

T

E

P

A

Q

F

M

K

L

Q

R

E

Q

G

-

H

R

G

S

G

G

K

A

I

I

N

N

A

L

C

S

S
|
S

Q

V

A

V

G

G

H

A

V

V

G

G

N

N

P

P

E

G

L
x
Q

A

A

V

N

Y
|
Y

S

V

S

A

S

T

K
|
K

F

A

A

G

V

V

R

I

G

G

L

L

T

T

Q

K

T

S

A

A

R

R

D

E

L

L

A

A

H

S

L

R

G

G

I

I

T

T

V

V

N

N

G

A

Y

V

C

A

P

P

G

G

I

F

V

I

K

V

T

S

P

D

M

M

W

T

A

D

E

A

I

L

D

S

R

D

Q

E

V

L

S

K

E

E

A

-

G

-

K

-

P

-

L

-

G

-

Y

-

G

-

T

-

Q

-

E

Q

F

M

A

L

K

T

R

Q

I

I

T

P

L

L

G

A

R

R

L

F

S

G

E

Q

P

D

E

T

D

D

V

I

A

A

A

N

C

T

V

V

S

A

Y

F

L

L

A

A

G

S

P

D

D

K

S

A

N

K

Y

Y

M

I

T

T

G

G

Q

Q

S

T

L

I

L

H

I

V

D

N

G

G

M

M

active site: G15 (= G13), S141 (= S139), Q151 (≠ L149), Y154 (= Y152), K158 (= K156)
binding magnesium ion: N89 (= N86), K158 (= K156)

3sj7A Structure of beta-ketoacetyl-coa reductase (fabg) from staphylococcus aureus complex with NADPH (see paper)
37% identity, 98% coverage: 3:253/256 of query aligns to 2:237/239 of 3sj7A

query
sites
3sj7A

K

K

V

S

A

A

L

L

V

V

T

T

G
|
G

A

A

G
x
S

Q
x
R

G
|
G

I
|
I

G

G

K

R

A

S

I

I

A

A

L

L

R

Q

L

L

V

A

K

E

D

E

G

G

F

Y

A

N

V

V

A

A

I

V

A

-

D
x
N

Y
|
Y

-
x
A

-
x
G

N
x
S

D

K

A

E

T

K

A

A

Q

E

A

A

V

V

A

V

D

E

E

E

I

I

N

K

R

A

S

K

G

G

G

V

R

D

A

S

L

F

A

A

V

I

K

Q

V
x
A

D
x
N

V
|
V

S

A

Q

D

R

A

D

D

Q

E

V

V

F

K

A

A

A

M

V

I

E

K

Q

E

A

V

R

V

K

S

G

Q

L

F

G

G

G

S

F

L

D

D

V

V

I

L

V

V

N

N

N
|
N

A
|
A

G
|
G

V
x
I

A

T

P

R

S

D

T

N

P

L

I

L

E

M

E

R

I

M

R

K

E

E

D

Q

V

E

I

W

D

D

K

D

V

V

Y

I

N

D

I
x
T

N

N

V

L

K

K

G

G

V

V

I

F

W

N

G

C

I

I

Q

Q

A

K

A

A

V

T

E

P

A

Q

F

M

K

L

Q

R

E

Q

G

-

H

R

G

S

G

G

K

A

I

I

N

N

A
x
L

C
x
S

S
|
S

Q

V

A

V

G

G

H

A

V

V

G

G

N

N

P

P

E

G

L
x
Q

A

A

V

N

Y
|
Y

S

V

S

A

S

T

K
|
K

F

A

A

G

V

V

R

I

G

G

L

L

T

T

Q

K

T

S

A

A

R

R

D

E

L

L

A

A

H

S

L

R

G

G

I

I

T

T

V

V

N

N

G

A

Y

V

C

A

P
|
P

G
|
G

I
x
F

V
x
I

K

V

T

S

P

D

M

M

W

T

A

D

E

E

I

L

D

K

R

E

Q

Q

V

-

S

-

E

-

A

-

G

-

K

-

P

-

L

-

G

-

Y

-

G

-

T

-

Q

-

E

-

F

M

A

L

K

T

R

Q

I

I

T

P

L

L

G

A

R

R

L

F

S

G

E

Q

P

D

E

T

D

D

V

I

A

A

A

N

C

T

V

V

S

A

Y

F

L

L

A

A

G

S

P

D

D

K

S

A

N

K

Y

Y

M

I

T

T

G

G

Q

Q

S

T

L

I

L

H

I

V

D

N

G

G

M

M

active site: G12 (= G13), S138 (= S139), Q148 (≠ L149), Y151 (= Y152), K155 (= K156)
binding nadph dihydro-nicotinamide-adenine-dinucleotide phosphate: G8 (= G9), S10 (≠ G11), R11 (≠ Q12), G12 (= G13), I13 (= I14), N31 (≠ D33), Y32 (= Y34), A33 (vs. gap), G34 (vs. gap), S35 (≠ N35), A58 (≠ V58), N59 (≠ D59), V60 (= V60), N86 (= N86), A87 (= A87), G88 (= G88), I89 (≠ V89), T109 (≠ I109), L136 (≠ A137), S137 (≠ C138), S138 (= S139), Y151 (= Y152), K155 (= K156), P181 (= P182), G182 (= G183), F183 (≠ I184), I184 (≠ V185)

4nbuB Crystal structure of fabg from bacillus sp (see paper)
35% identity, 99% coverage: 3:255/256 of query aligns to 8:244/244 of 4nbuB

query
sites
4nbuB

K

K

V

V

A

A

L

I

V

I

T

T

G
|
G

A

A

G
x
A

Q
x
N

G
|
G

I
|
I

G

G

K

L

A

E

I

A

A

A

L

R

R

V

L

F

V

M

K

K

D

E

G

G

F

A

A

K

V

V

A

V

I

I

A

A

D
|
D

Y
x
F

N
|
N

D

E

A

A

T

A

A

G

Q

K

A

-

V

-

A

-

D

-

E

E

I

A

N

V

R

E

S

A

G

N

G

P

R

G

A

V

L

V

A

F

V

I

K

R

V
|
V

D
|
D

V
|
V

S

S

Q

D

R

R

D

E

Q

S

V

V

F

H

A

R

A

L

V

V

E

E

Q

N

A

V

R

A

K

E

G

R

L

F

G

G

G

K

F

I

D

D

V

I

I

L

V

I

N

N

N
|
N

A
|
A

G
|
G

V
x
I

A
x
T

P

R

S
x
D

T

S

P

M

I

L

E

S

E
x
K

I

M

R

T

E

V

D

D

V

Q

I

F

D

Q

K

Q

V

V

Y

I

N

N

I
x
V

N
|
N

V

L

K

T

G

G

V

V

I

F

W

H

G

C

I

T

Q

Q

A

A

A

V

V

L

E

P

A

Y

F

M

K

A

Q

E

E

Q

G

G

H

K

G

G

G

-

K

K

I

I

N

N

A
x
T

C
x
S

S
|
S

Q

V

A
x
T

G

G

H

T

V

Y

G

G

N
|
N

P
x
V

E
x
G

L
x
Q

A

T

V

N

Y
|
Y

S

A

K
|
K

F

A

A

G

V

V

R

I

G

G

L

M

T

T

Q

K

T

T

A

W

A

A

R

K

D

E

L

L

A

A

H

R

L

K

G

G

I

I

T

N

V

V

N

N

G

A

Y

V

C

A

P
|
P

G
|
G

I
x
F

V
x
T

K

E

T
|
T

P
x
A

M
|
M

W
x
V

A

A

E

E

I

V

D

P

R

E

Q

K

V

V

S

I

E

E

A

-

G

-

K

-

P

-

L

-

G

-

Y

-

G

-

T

-

Q

-

E
x
K

F

M

A

K

K

A

R

Q

I

V

T

P

L

M

G

G

R

R

L

L

S

G

E

K

P

P

E

E

D

D

V

I

A

A

A

N

C

A

V

Y

S

L

Y

F

L

L

A

A

G

S

P

H

D

E

S

S

N

D

Y

Y

M

V

T

N

G

G

Q

H

S

V

L

L

L

H

I

V

D

D

G

G

M

I

V

M

F

M

active site: G18 (= G13), N111 (= N110), S139 (= S139), Q149 (≠ L149), Y152 (= Y152), K156 (= K156)
binding acetoacetyl-coenzyme a: T91 (≠ A90), D93 (≠ S92), K98 (≠ E97), S139 (= S139), T141 (≠ A141), N146 (= N146), V147 (≠ P147), G148 (≠ E148), Q149 (≠ L149), Y152 (= Y152), F184 (≠ I184), M189 (= M189), K200 (≠ E211)
binding 1,4-dihydronicotinamide adenine dinucleotide: G14 (= G9), A16 (≠ G11), N17 (≠ Q12), G18 (= G13), I19 (= I14), D38 (= D33), F39 (≠ Y34), N40 (= N35), V59 (= V58), D60 (= D59), V61 (= V60), N87 (= N86), A88 (= A87), G89 (= G88), I90 (≠ V89), V110 (≠ I109), T137 (≠ A137), S138 (≠ C138), S139 (= S139), Y152 (= Y152), K156 (= K156), P182 (= P182), G183 (= G183), F184 (≠ I184), T185 (≠ V185), T187 (= T187), A188 (≠ P188), M189 (= M189), V190 (≠ W190)

4cqlI Crystal structure of heterotetrameric human ketoacyl reductase complexed with NAD (see paper)
37% identity, 98% coverage: 4:255/256 of query aligns to 10:251/251 of 4cqlI

query
sites
4cqlI

V

L

A

A

L

L

V

V

T

T

G
|
G

A

A

G
|
G

Q
x
S

G
|
G

I
|
I

G

G

K

R

A

A

I

V

A

S

L

V

R

R

L

L

V

A

K

G

D

E

G

G

F

A

A

T

V

V

A

A

I

A

A

C

D
|
D

Y
x
L

N

D

D

R

A

A

T

A

A

A

Q

Q

A

E

V

T

A

V

D

R

E

L

I

L

N

G

R

G

S

P

G

G

G

S

R

N

A

H

L

A

A

A

V

F

K

Q

V
x
A

D
|
D

V
|
V

S

S

Q

E

R

A

D

R

Q

A

V

A

F

R

A

C

A

L

V

L

E

E

Q

Q

A

V

R

Q

K

A

G

C

L

F

G

S

-

R

G

P

F

P

D

S

V

V

I

V

V

V

N

S

N
x
C

A
|
A

G
|
G

V
x
I

A

T

P

Q

S

D

T

E

P

F

I

L

E

L

E

H

I

M

R

S

E

E

D

D

V

D

I

W

D

D

K

K

V

V

Y

I

N

A

I
x
V

N

N

V

L

K

K

G

G

V

T

I

F

W

L

G

V

I

T

Q

Q

A

A

V

A

E

Q

A

A

F

L

K

V

Q

S

E

N

G

G

H

C

G

R

G

G

K

S

I

I

N

N

A
x
I

C

S

S
|
S

Q

I

A

V

G

G

H

K

V

V

G

G

N

N

P

V

E

G

L

Q

A

T

V

N

Y
|
Y

S

A

S

S

K
|
K

F

A

A

G

V

V

R

I

G

G

L

L

T

T

Q

Q

T

T

A

A

R

R

D

E

L

L

A

G

H

R

L

H

G

G

I

I

T

R

V

C

N

N

G

S

Y

V

C

L

P
|
P

G
|
G

I
x
F

V
x
I

K

A

T
|
T

P
|
P

M
|
M

W
x
T

A

Q

E

K

I

V

D

P

R

Q

Q

K

V

V

S

V

E

D

A

-

G

-

K

-

P

-

L

-

G

-

Y

-

G

-

T

-

Q

-

E

K

F

I

A

T

K

E

R

M

I

I

T

P

L

M

G

G

R

H

L

L

S

G

E

D

P

P

E

E

D

D

V

V

A

A

A

D

C

V

V

V

S

A

Y

F

L

L

A

A

G

S

P

E

D

D

S

S

N

G

Y

Y

M

I

T

T

G

G

Q

T

S

S

L

V

L

E

I

V

D

T

G

G

M

L

V

F

F

M

active site: G19 (= G13), S146 (= S139), Y159 (= Y152), K163 (= K156)
binding nicotinamide-adenine-dinucleotide: G15 (= G9), G17 (= G11), S18 (≠ Q12), G19 (= G13), I20 (= I14), D39 (= D33), L40 (≠ Y34), A64 (≠ V58), D65 (= D59), V66 (= V60), C93 (≠ N86), A94 (= A87), G95 (= G88), I96 (≠ V89), V116 (≠ I109), I144 (≠ A137), S146 (= S139), Y159 (= Y152), K163 (= K156), P189 (= P182), G190 (= G183), F191 (≠ I184), I192 (≠ V185), T194 (= T187), P195 (= P188), M196 (= M189), T197 (≠ W190)

4jroC Crystal structure of 3-oxoacyl-[acyl-carrier protein]reductase (fabg) from listeria monocytogenes in complex with NADP+
39% identity, 99% coverage: 3:255/256 of query aligns to 6:247/247 of 4jroC

query
sites
4jroC

K

K

V

V

A

A

L

V

V

V

T

T

G
|
G

A

G

G
x
S

Q
x
R

G
|
G

I
|
I

G

G

K

R

A

D

I

I

A

A

L

I

R

N

L

L

V

A

K

K

D

E

G

G

F

-

A

A

V

N

A

I

I

F

A

F

D
x
N

Y
|
Y

N
|
N

D
x
G

A
x
S

-

P

-

E

T

A

A

A

Q

E

A

E

V

T

A

A

D

K

E

L

I

V

N

A

R

E

S

H

G

G

G

V

R

E

A

V

L

E

A

A

V

M

K

K

V
x
A

D
x
N

V
|
V

S

A

Q

I

R

A

D

E

Q

D

V

V

F

D

A

A

A

F

V

F

E

K

Q

Q

A

A

R

I

K

E

G

R

L

F

G

G

G

R

F

V

D

D

V

I

I

L

V

V

N

N

N
|
N

A
|
A

G
|
G

V
x
I

A

T

P

R

S

D

T

N

P

L

I

L

E

M

E

R

I

M

R

K

E

E

D

D

V

E

I

W

D

D

K

D

V

V

Y

I

N

N

I
|
I

N

N

V

L

K

K

G

G

V

T

I

F

W

L

G

C

I

T

Q

K

A

A

A

V

V

S

E

R

A

T

F

M

K

M

Q

K

E

Q

G

-

H

R

G

A

G

G

K

K

I

I

N

N

A

M

C
x
A

S
|
S

Q

V

A

V

G

G

H

L

V

I

G

G

N

N

P

A

E

G

L
x
Q

A

A

V

N

Y
|
Y

S

V

S

A

S

S

K
|
K

F

A

A

G

V

V

R

I

G

G

L

L

T

T

Q

K

T

T

A

T

A

A

R

R

D

E

L

L

A

A

H

P

L

R

G

G

I

I

T

N

V

V

N

N

G

A

Y

V

C

A

P
|
P

G
|
G

I

F

V
x
I

K

T

T
|
T

P

D

M

M

W

T

A

D

E

K

I

L

D

D

R

E

Q

K

V

T

S

K

E

E

A

A

-

M

-

L

A

A

G

Q

K

I

P

P

L

L

G

G

-

A

Y

Y

G

G

T

T

Q

-

E

-

F

-

A

-

K

-

R

-

I

-

T

-

L

-

G

-

R

-

L

-

S

-

E

-

P

T

E

E

D

D

V

I

A

A

A

N

C

A

V

V

S

L

Y

F

L

L

A

A

G

S

P

D

D

A

S

S

N

K

Y

Y

M

I

T

T

G

G

Q

Q

S

T

L

L

L

S

I

V

D

D

G

G

M

M

V

V

F

M

active site: G16 (= G13), S142 (= S139), Q152 (≠ L149), Y155 (= Y152), K159 (= K156)
binding nadp nicotinamide-adenine-dinucleotide phosphate: G12 (= G9), S14 (≠ G11), R15 (≠ Q12), G16 (= G13), I17 (= I14), N35 (≠ D33), Y36 (= Y34), N37 (= N35), G38 (≠ D36), S39 (≠ A37), A62 (≠ V58), N63 (≠ D59), V64 (= V60), N90 (= N86), A91 (= A87), G92 (= G88), I93 (≠ V89), I113 (= I109), A141 (≠ C138), S142 (= S139), Y155 (= Y152), K159 (= K156), P185 (= P182), G186 (= G183), I188 (≠ V185), T190 (= T187)

3iccA Crystal structure of a putative 3-oxoacyl-(acyl carrier protein) reductase from bacillus anthracis at 1.87 a resolution (see paper)
36% identity, 98% coverage: 3:252/256 of query aligns to 8:252/255 of 3iccA

query
sites
3iccA

K

K

V

V

A

A

L

L

V

V

T

T

G
|
G

A
|
A

G
x
S

Q
x
R

G
|
G

I
|
I

G

G

K

R

A

A

I

I

A

A

L

K

R

R

L

L

V

A

K

N

D

D

G

G

F

A

A

L

V

V

A

A

I

I

A
x
H

D
x
Y

Y
x
G

N
|
N

-

R

D

K

A

E

T

E

A

A

Q

E

A

E

V

T

A

V

D

Y

E

E

I

I

N

Q

R

S

S

N

G

G

R

S

A

A

L

F

A

S

V

I

K

G

V

A

D

N

V
x
L

S
x
E

Q

S

R

L

-

H

-

G

-

V

D

E

Q

A

V

L

F

Y

A

S

V

L

E

D

-

N

-

E

-

L

Q

Q

A

N

R

R

K

T

G

G

L

S

G

T

G

K

F

F

D

D

V

I

I

L

V

I

N

N

N
|
N

A
|
A

G
|
G

V
x
I

A

G

P

P

S

G

T

A

P

F

I

I

E

E

I

T

R

T

E

E

D

Q

V

F

I

F

D

D

K

R

V

M

Y

V

N

S

I
x
V

N

N

V

A

K

K

G

A

V

P

I

F

W

F

G

I

I

I

Q

Q

A

Q

A

A

V

L

E

S

A

R

F

L

K

R

Q

D

E

N

G

S

H

-

G

-

K

R

I

I

N

N

A
x
I

C
x
S

S
|
S

Q

A

A

A

G

T

H

R

V

I

G

S

N

L

P

P

E

D

L
x
F

A

I

V

A

Y
|
Y

S

S

S

M

S

T

K
|
K

F

G

A

A

V

I

R

N

G

T

L

M

T

T

Q

F

T

T

A

L

A

A

R

K

D

Q

L

L

A

G

H

A

L

R

G

G

I

I

T

T

V

V

N

N

G

A

Y

I

C

L

P
|
P

G
|
G

I
x
F

V
|
V

K

K

T
|
T

P

D

M
|
M

W
x
N

A

A

E

E

I

L

D

-

R

-

Q

-

V

-

S

-

E

-

A

-

A

L

G

S

K

D

P

P

L

M

G

-

Y

-

G

-

T

M

Q

K

E

Q

F

Y

A

A

K

T

R

T

I

I

T

S

-

A

L

F

G

N

R

R

L

L

S

G

E

E

P

V

E

E

D

D

V

I

A

A

A

D

C

T

V

A

S

A

Y

F

L

L

A

A

G

S

P

P

D

D

S

S

N

R

Y

W

M

V

T

T

G

G

Q

Q

S

L

L

I

L

D

I

V

D

S

G

G

active site: G18 (= G13), S148 (= S139), F158 (≠ L149), Y161 (= Y152), K165 (= K156)
binding nadp nicotinamide-adenine-dinucleotide phosphate: G14 (= G9), A15 (= A10), S16 (≠ G11), R17 (≠ Q12), G18 (= G13), I19 (= I14), H37 (≠ A32), Y38 (≠ D33), G39 (≠ Y34), N40 (= N35), L66 (≠ V60), E67 (≠ S61), N98 (= N86), A99 (= A87), G100 (= G88), I101 (≠ V89), V121 (≠ I109), I146 (≠ A137), S147 (≠ C138), S148 (= S139), Y161 (= Y152), K165 (= K156), P191 (= P182), G192 (= G183), F193 (≠ I184), V194 (= V185), T196 (= T187), M198 (= M189), N199 (≠ W190)

4cqmA Crystal structure of heterotetrameric human ketoacyl reductase complexed with NAD and NADP (see paper)
38% identity, 98% coverage: 4:255/256 of query aligns to 8:248/248 of 4cqmA

query
sites
4cqmA

V

L

A

A

L

L

V

V

T

T

G
|
G

A

A

G
|
G

Q
x
S

G
|
G

I
|
I

G

G

K

R

A

A

I

V

A

S

L

V

R

R

L

L

V

A

K

G

D

E

G

G

F

A

A

T

V

V

A

A

I

A

A

C

D
|
D

Y
x
L

N

D

D

R

A

A

T

A

A

A

Q

Q

A

E

V

T

A

V

D

R

E

L

I

L

N

P

R

P

S

R

G

G

G

N

R

H

A

A

L

-

A

A

V

F

K

Q

V
x
A

D
|
D

V
|
V

S

S

Q

E

R

A

D

R

Q

A

V

A

F

R

A

C

A

L

V

L

E

E

Q

Q

A

V

R

Q

K

A

G

C

L

F

G

S

-

R

G

P

F

P

D

S

V

V

I

V

V

V

N

S

N
x
C

A
|
A

G
|
G

V
x
I

A

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P

Q

S

D

T

E

P

F

I

L

E

L

E

H

I

M

R

S

E

E

D

D

V

D

I

W

D

D

K

K

V

V

Y

I

N

A

I
x
V

N

N

V

L

K

K

G

G

V

T

I

F

W

L

G

V

I

T

Q

Q

A

A

V

A

E

Q

A

A

F

L

K

V

Q

S

E

N

G

G

H

C

G

R

G

G

K

S

I

I

N

N

A
x
I

C
x
S

S
|
S

Q

I

A

V

G

G

H

K

V

V

G

G

N

N

P

V

E

G

L

Q

A

T

V

N

Y
|
Y

S

A

S

S

K
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K

F

A

A

G

V

V

R

I

G

G

L

L

T

T

Q

Q

T

T

A

A

R

R

D

E

L

L

A

G

H

R

L

H

G

G

I

I

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R

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C

N

N

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P

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G

I
x
F

V
x
I

K

A

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T

P
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P

M
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M

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x
T

A

Q

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K

I

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P

R

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Q

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V

V

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V

E

D

A

-

G

-

K

-

P

-

L

-

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-

Y

-

G

-

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-

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-

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K

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K

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P

E

E

D

D

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V

A

A

A

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C

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V

V

S

A

Y

F

L

L

A

A

G

S

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E

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D

S

S

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G

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Y

M

I

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T

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V

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G

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M

active site: G17 (= G13), S143 (= S139), Y156 (= Y152), K160 (= K156)
binding nadp nicotinamide-adenine-dinucleotide phosphate: G13 (= G9), G15 (= G11), S16 (≠ Q12), G17 (= G13), I18 (= I14), D37 (= D33), L38 (≠ Y34), A61 (≠ V58), D62 (= D59), V63 (= V60), C90 (≠ N86), A91 (= A87), G92 (= G88), I93 (≠ V89), V113 (≠ I109), I141 (≠ A137), S142 (≠ C138), S143 (= S139), Y156 (= Y152), K160 (= K156), P186 (= P182), G187 (= G183), F188 (≠ I184), I189 (≠ V185), T191 (= T187), P192 (= P188), M193 (= M189), T194 (≠ W190)

7tzpG Crystal structure of putataive short-chain dehydrogenase/reductase (fabg) from klebsiella pneumoniae subsp. Pneumoniae ntuh-k2044 in complex with nadh (see paper)
36% identity, 99% coverage: 3:255/256 of query aligns to 9:247/247 of 7tzpG

query
sites
7tzpG

K

K

V

T

A

A

L

I

V

V

T

T

G
|
G

A

A

G
x
A

Q
x
R

G
|
G

I
|
I

G

G

K

F

A

G

I

I

A

A

L

Q

R

V

L

L

V

A

K

R

D

E

G

G

F

A

A

R

V

V

A

I

I

I

A

A

D
|
D

Y

-

N
x
R

D

D

A

A

T

H

A

G

Q

E

A

A

V

A

A

A

D

A

E

S

I

L

N

R

R

E

S

S

G

G

G

A

R

Q

A

A

L

L

A

F

V

I

K

S

V
x
C

D
x
N

V
x
I

S

A

Q

E

R

K

D

T

Q

Q

V

V

F

E

A

A

A

L

V

F

E

S

Q

Q

A

A

R

E

K

E

G

A

L

F

G

G

G

P

F

V

D

D

V

I

I

L

V

V

N

N

N
|
N

A
|
A

G
|
G

V
x
I

A

N

P

R

S

D

T

A

P

M

I

L

E

H

E

K

I

L

R

T

E

E

D

A

V

D

I

W

D

D

K

T

V

V

Y

I

N

D

I
x
V

N

N

V

L

K

K

G

G

V

T

I

F

W

L

G

C

I

M

Q

Q

A

Q

A

A

V

A

E

I

A

R

F

M

K

R

Q

E

E

R

G

G

H

-

G

A

G

G

K

R

I

I

N

N

A
x
I

C

A

S
|
S

Q

-

A

A

G

S

H

W

V

L

G

G

N

N

P

V

E

G

L

Q

A

T

V

N

Y
|
Y

S

S

S

A

S

S

K
|
K

F

A

A

G

V

V

R

V

G

G

L

M

T

T

Q

K

T

T

A

A

A

C

R

R

D

E

L

L

A

A

H

K

L

K

G

G

I

V

T

T

V

V

N

N

G

A

Y

I

C

C

P
|
P

G
|
G

I
x
F

V
x
I

K

D

T
|
T

P

D

M
|
M

W
x
T

A

R

E

G

I

V

D

P

R

E

Q

N

V

V

S

-

E

-

A

-

G

-

K

-

P

-

L

-

G

-

Y

-

G

-

T

W

Q

Q

E

I

F

M

A

V

K

S

R

K

I

I

T

P

L

A

G

G

R

Y

L

A

S

G

E

E

P

A

E

K

D

D

V

V

A

G

A

E

C

C

V

V

S

A

Y

F

L

L

A

A

G

S

P

D

D

G

S

A

N

R

Y

Y

M

I

T

N

G

G

Q

E

S

V

L

I

L

N

I

V

D

G

G

G

M

M

V

V

F

L

binding 1,4-dihydronicotinamide adenine dinucleotide: G15 (= G9), A17 (≠ G11), R18 (≠ Q12), G19 (= G13), I20 (= I14), D39 (= D33), R40 (≠ N35), C63 (≠ V58), N64 (≠ D59), I65 (≠ V60), N91 (= N86), A92 (= A87), G93 (= G88), I94 (≠ V89), V114 (≠ I109), I141 (≠ A137), S143 (= S139), Y155 (= Y152), K159 (= K156), P185 (= P182), G186 (= G183), F187 (≠ I184), I188 (≠ V185), T190 (= T187), M192 (= M189), T193 (≠ W190)

Q92506 (3R)-3-hydroxyacyl-CoA dehydrogenase; 17-beta-hydroxysteroid dehydrogenase 8; 17-beta-HSD 8; HSD17B8; 3-ketoacyl-[acyl-carrier-protein] reductase alpha subunit; KAR alpha subunit; 3-oxoacyl-[acyl-carrier-protein] reductase; Estradiol 17-beta-dehydrogenase 8; Protein Ke6; Ke6; Short chain dehydrogenase/reductase family 30C member 1; Testosterone 17-beta-dehydrogenase 8; EC 1.1.1.n12; EC 1.1.1.62; EC 1.1.1.239 from Homo sapiens (Human) (see 2 papers)
37% identity, 98% coverage: 4:255/256 of query aligns to 13:261/261 of Q92506

query
sites
Q92506

V

L

A

A

L
|
L

V
|
V

T
|
T

G
|
G

A
|
A

G
|
G

Q
x
S

G
|
G

I
|
I

G

G

K

R

A

A

I

V

A

S

L

V

R

R

L

L

V

A

K

G

D

E

G

G

F

A

A

T

V

V