SitesBLAST
Comparing BWI76_RS16755 FitnessBrowser__Koxy:BWI76_RS16755 to proteins with known functional sites using BLASTp with E ≤ 0.001.
Or try Sites on a Tree, PaperBLAST, Conserved Domains, or compare to all protein structures
Found 20 (the maximum) hits to proteins with known functional sites (download)
Q48436 Diacetyl reductase [(S)-acetoin forming]; Acetoin(diacetyl) reductase; AR; Meso-2,3-butanediol dehydrogenase; EC 1.1.1.304 from Klebsiella pneumoniae (see paper)
93% identity, 100% coverage: 1:256/256 of query aligns to 1:256/256 of Q48436
- 6:33 (vs. 6:33, 100% identical) binding
- D59 (= D59) binding
- K156 (= K156) binding
1gegE Cryatal structure analysis of meso-2,3-butanediol dehydrogenase (see paper)
93% identity, 100% coverage: 1:256/256 of query aligns to 1:256/256 of 1gegE
- active site: G13 (= G13), S139 (= S139), Y152 (= Y152), K156 (= K156), V197 (= V197)
- binding alpha-D-glucopyranose: R63 (= R63), D64 (= D64), F67 (= F67), E123 (= E123)
- binding nicotinamide-adenine-dinucleotide: G9 (= G9), Q12 (= Q12), I14 (= I14), D33 (= D33), Y34 (= Y34), V58 (= V58), D59 (= D59), V60 (= V60), N86 (= N86), A87 (= A87), I109 (= I109), S139 (= S139), Y152 (= Y152), K156 (= K156), P182 (= P182), V185 (= V185), T187 (= T187), M189 (= M189)
3wyeA Crystal structure of chimeric engineered (2s,3s)-butanediol dehydrogenase complexed with NAD+
73% identity, 100% coverage: 2:256/256 of query aligns to 1:255/255 of 3wyeA
- active site: G12 (= G13), S138 (= S139), Y151 (= Y152), K155 (= K156), L196 (≠ V197)
- binding nicotinamide-adenine-dinucleotide: G8 (= G9), Q11 (= Q12), G12 (= G13), I13 (= I14), D32 (= D33), Y33 (= Y34), V57 (= V58), D58 (= D59), V59 (= V60), N85 (= N86), A86 (= A87), S138 (= S139), Y151 (= Y152), K155 (= K156), P181 (= P182), G182 (= G183), V184 (= V185), T186 (= T187), M188 (= M189), W189 (= W190)
Q9ZNN8 L-2,3-butanediol dehydrogenase; L-BDH; (S,S)-butanediol dehydrogenase; Diacetyl reductase [(S)-acetoin forming]; EC 1.1.1.76; EC 1.1.1.304 from Corynebacterium glutamicum (Brevibacterium saccharolyticum) (see paper)
49% identity, 100% coverage: 1:256/256 of query aligns to 1:258/258 of Q9ZNN8
- QGI 12:14 (= QGI 12:14) binding
- D33 (= D33) binding
- Q37 (≠ A37) binding
- DV 61:62 (= DV 59:60) binding
- N88 (= N86) binding
- I142 (≠ Q140) mutation to Q: Loss of L-BD oxidizing activity, and does not gain the ability to use meso-BD as substrate; when associated with N-148.; mutation to Q: Loss of L-BD oxidizing activity. Does not gain the ability to use meso-BD as substrate.
- F148 (≠ N146) mutation to N: Loss of L-BD oxidizing activity, and does not gain the ability to use meso-BD as substrate; when associated with Q-142.; mutation to N: Loss of L-BD oxidizing activity. Does not gain the ability to use meso-BD as substrate.
- Y154 (= Y152) binding
- K158 (= K156) binding
- PGIVGT 184:189 (≠ PGIVKT 182:187) binding
3a28C Crystal structure of l-2,3-butanediol dehydrogenase (see paper)
49% identity, 99% coverage: 3:256/256 of query aligns to 2:257/257 of 3a28C
- active site: G12 (= G13), S140 (= S139), Y153 (= Y152), K157 (= K156), L198 (≠ V197)
- binding nicotinamide-adenine-dinucleotide: G8 (= G9), Q11 (= Q12), I13 (= I14), D32 (= D33), L33 (≠ Y34), Q36 (≠ A37), L59 (≠ V58), D60 (= D59), V61 (= V60), N87 (= N86), S140 (= S139), Y153 (= Y152), K157 (= K156), P183 (= P182), V186 (= V185), T188 (= T187), M190 (= M189), W191 (= W190)
3ak4A Crystal structure of nadh-dependent quinuclidinone reductase from agrobacterium tumefaciens
42% identity, 97% coverage: 5:253/256 of query aligns to 10:255/258 of 3ak4A
- active site: G18 (= G13), S141 (= S139), L151 (= L149), Y154 (= Y152), K158 (= K156), E199 (vs. gap)
- binding nicotinamide-adenine-dinucleotide: K17 (≠ Q12), G18 (= G13), I19 (= I14), D38 (= D33), L39 (≠ Y34), V60 (= V58), D61 (= D59), V62 (= V60), N88 (= N86), A89 (= A87), G90 (= G88), T139 (≠ A137), S141 (= S139), Y154 (= Y152), K158 (= K156), G185 (= G183), V187 (= V185), T189 (= T187), M191 (= M189)
6pejA Structure of sorbitol dehydrogenase from sinorhizobium meliloti 1021 bound to sorbitol
38% identity, 98% coverage: 3:252/256 of query aligns to 7:253/257 of 6pejA
3osuA Crystal structure of the 3-oxoacyl-acyl carrier protein reductase, fabg, from staphylococcus aureus
37% identity, 99% coverage: 1:253/256 of query aligns to 3:244/246 of 3osuA
3sj7A Structure of beta-ketoacetyl-coa reductase (fabg) from staphylococcus aureus complex with NADPH (see paper)
37% identity, 98% coverage: 3:253/256 of query aligns to 2:237/239 of 3sj7A
- active site: G12 (= G13), S138 (= S139), Q148 (≠ L149), Y151 (= Y152), K155 (= K156)
- binding nadph dihydro-nicotinamide-adenine-dinucleotide phosphate: G8 (= G9), S10 (≠ G11), R11 (≠ Q12), I13 (= I14), N31 (≠ D33), Y32 (= Y34), A33 (vs. gap), G34 (vs. gap), S35 (≠ N35), A58 (≠ V58), N59 (≠ D59), V60 (= V60), N86 (= N86), A87 (= A87), T109 (≠ I109), S138 (= S139), Y151 (= Y152), K155 (= K156), P181 (= P182), G182 (= G183)
4nbuB Crystal structure of fabg from bacillus sp (see paper)
35% identity, 99% coverage: 3:255/256 of query aligns to 8:244/244 of 4nbuB
- active site: G18 (= G13), N111 (= N110), S139 (= S139), Q149 (≠ L149), Y152 (= Y152), K156 (= K156)
- binding acetoacetyl-coenzyme a: D93 (≠ S92), K98 (≠ E97), S139 (= S139), N146 (= N146), V147 (≠ P147), Q149 (≠ L149), Y152 (= Y152), F184 (≠ I184), M189 (= M189), K200 (≠ E211)
- binding 1,4-dihydronicotinamide adenine dinucleotide: G14 (= G9), N17 (≠ Q12), G18 (= G13), I19 (= I14), D38 (= D33), F39 (≠ Y34), V59 (= V58), D60 (= D59), V61 (= V60), N87 (= N86), A88 (= A87), G89 (= G88), I90 (≠ V89), T137 (≠ A137), S139 (= S139), Y152 (= Y152), K156 (= K156), P182 (= P182), F184 (≠ I184), T185 (≠ V185), T187 (= T187), M189 (= M189)
4cqlI Crystal structure of heterotetrameric human ketoacyl reductase complexed with NAD (see paper)
37% identity, 98% coverage: 4:255/256 of query aligns to 10:251/251 of 4cqlI
- active site: G19 (= G13), S146 (= S139), Y159 (= Y152), K163 (= K156)
- binding nicotinamide-adenine-dinucleotide: S18 (≠ Q12), G19 (= G13), I20 (= I14), D39 (= D33), L40 (≠ Y34), A64 (≠ V58), D65 (= D59), V66 (= V60), C93 (≠ N86), A94 (= A87), G95 (= G88), I96 (≠ V89), V116 (≠ I109), I144 (≠ A137), S146 (= S139), Y159 (= Y152), K163 (= K156), P189 (= P182), G190 (= G183), I192 (≠ V185), T194 (= T187), M196 (= M189)
4jroC Crystal structure of 3-oxoacyl-[acyl-carrier protein]reductase (fabg) from listeria monocytogenes in complex with NADP+
39% identity, 99% coverage: 3:255/256 of query aligns to 6:247/247 of 4jroC
- active site: G16 (= G13), S142 (= S139), Q152 (≠ L149), Y155 (= Y152), K159 (= K156)
- binding nadp nicotinamide-adenine-dinucleotide phosphate: G12 (= G9), S14 (≠ G11), R15 (≠ Q12), G16 (= G13), I17 (= I14), N35 (≠ D33), Y36 (= Y34), N37 (= N35), G38 (≠ D36), S39 (≠ A37), N63 (≠ D59), V64 (= V60), N90 (= N86), A91 (= A87), I93 (≠ V89), I113 (= I109), S142 (= S139), Y155 (= Y152), K159 (= K156), P185 (= P182), I188 (≠ V185), T190 (= T187)
3iccA Crystal structure of a putative 3-oxoacyl-(acyl carrier protein) reductase from bacillus anthracis at 1.87 a resolution (see paper)
36% identity, 98% coverage: 3:252/256 of query aligns to 8:252/255 of 3iccA
- active site: G18 (= G13), S148 (= S139), F158 (≠ L149), Y161 (= Y152), K165 (= K156)
- binding nadp nicotinamide-adenine-dinucleotide phosphate: G14 (= G9), S16 (≠ G11), R17 (≠ Q12), G18 (= G13), I19 (= I14), H37 (≠ A32), Y38 (≠ D33), G39 (≠ Y34), L66 (≠ V60), E67 (≠ S61), N98 (= N86), G100 (= G88), I146 (≠ A137), S148 (= S139), Y161 (= Y152), K165 (= K156), P191 (= P182), G192 (= G183), M198 (= M189), N199 (≠ W190)
4cqmA Crystal structure of heterotetrameric human ketoacyl reductase complexed with NAD and NADP (see paper)
38% identity, 98% coverage: 4:255/256 of query aligns to 8:248/248 of 4cqmA
- active site: G17 (= G13), S143 (= S139), Y156 (= Y152), K160 (= K156)
- binding nadp nicotinamide-adenine-dinucleotide phosphate: G13 (= G9), S16 (≠ Q12), G17 (= G13), I18 (= I14), D37 (= D33), L38 (≠ Y34), A61 (≠ V58), V63 (= V60), C90 (≠ N86), A91 (= A87), G92 (= G88), I93 (≠ V89), V113 (≠ I109), I141 (≠ A137), S143 (= S139), Y156 (= Y152), K160 (= K156), P186 (= P182), G187 (= G183), I189 (≠ V185), T191 (= T187), P192 (= P188), M193 (= M189), T194 (≠ W190)
7tzpG Crystal structure of putataive short-chain dehydrogenase/reductase (fabg) from klebsiella pneumoniae subsp. Pneumoniae ntuh-k2044 in complex with nadh (see paper)
36% identity, 99% coverage: 3:255/256 of query aligns to 9:247/247 of 7tzpG
- binding 1,4-dihydronicotinamide adenine dinucleotide: G15 (= G9), R18 (≠ Q12), G19 (= G13), I20 (= I14), D39 (= D33), R40 (≠ N35), C63 (≠ V58), I65 (≠ V60), N91 (= N86), G93 (= G88), I94 (≠ V89), V114 (≠ I109), Y155 (= Y152), K159 (= K156), I188 (≠ V185), T190 (= T187), T193 (≠ W190)
Q92506 (3R)-3-hydroxyacyl-CoA dehydrogenase; 17-beta-hydroxysteroid dehydrogenase 8; 17-beta-HSD 8; HSD17B8; 3-ketoacyl-[acyl-carrier-protein] reductase alpha subunit; KAR alpha subunit; 3-oxoacyl-[acyl-carrier-protein] reductase; Estradiol 17-beta-dehydrogenase 8; Protein Ke6; Ke6; Short chain dehydrogenase/reductase family 30C member 1; Testosterone 17-beta-dehydrogenase 8; EC 1.1.1.n12; EC 1.1.1.62; EC 1.1.1.239 from Homo sapiens (Human) (see 2 papers)
37% identity, 98% coverage: 4:255/256 of query aligns to 13:261/261 of Q92506
- 15:23 (vs. 6:14, 89% identical) binding
- D42 (= D33) mutation to A: Reduced NADH-dependent reductase activity with acetoacetyl-CoA. Reduced NADH-dependent reductase activity with 9,10-phenanthrene quinone. Increases NADPH-dependent reductase activities. No effect on the ability to restore growth of an OAR1-deficient yeast mutant.
- DL 42:43 (≠ DY 33:34) binding
- ADV 74:76 (≠ VDV 58:60) binding
- R148 (≠ G131) mutation to E: No effect on the ability to restore growth of an OAR1-deficient yeast mutant.
- V158 (≠ A141) to L: in a breast cancer sample; somatic mutation
- Y169 (= Y152) mutation to A: Strongly reduced NADH-dependent reductase activity with acetoacetyl-CoA. Strongly reduced NADH-dependent reductase activity with 9,10-phenanthrene quinone. Decreases NADPH-dependent reductase activity with acetoacetyl-CoA, but increases NADPH-dependent reductase activity with 9,10-phenanthrene quinone. No effect on the ability to restore growth of an OAR1-deficient yeast mutant.
- YAASK 169:173 (≠ YSSSK 152:156) binding
- K173 (= K156) mutation to A: Abolishes NADH-dependent reductase activity with acetoacetyl-CoA. Strongly reduced NADH-dependent reductase activity with 9,10-phenanthrene quinone. Slightly decreases NADPH-dependent reductase activity with acetoacetyl-CoA, but increases NADPH-dependent reductase activity with 9,10-phenanthrene quinone. No effect on the ability to restore growth of an OAR1-deficient yeast mutant.
- R189 (≠ H172) mutation to E: No effect on the ability to restore growth of an OAR1-deficient yeast mutant.
- IAT 202:204 (≠ VKT 185:187) binding
Q9KJF1 (2S)-[(R)-hydroxy(phenyl)methyl]succinyl-CoA dehydrogenase subunit BbsD; (S,R)-2-(alpha-hydroxybenzyl)succinyl-CoA dehydrogenase subunit BbsD; EC 1.1.1.429 from Thauera aromatica (see 2 papers)
35% identity, 98% coverage: 2:252/256 of query aligns to 5:242/248 of Q9KJF1
- S15 (≠ Q12) binding
- D36 (= D33) binding
- D62 (= D59) binding
- I63 (≠ V60) binding
- N89 (= N86) binding
- Y153 (= Y152) binding
- K157 (= K156) binding
Sites not aligning to the query:
- 1 modified: Initiator methionine, Removed
7pcsB Structure of the heterotetrameric sdr family member bbscd (see paper)
35% identity, 98% coverage: 2:252/256 of query aligns to 4:241/247 of 7pcsB
- binding nicotinamide-adenine-dinucleotide: G11 (= G9), M16 (≠ I14), D35 (= D33), I36 (≠ Y34), I62 (≠ V60), N88 (= N86), G90 (= G88), I138 (≠ A137), S140 (= S139), Y152 (= Y152), K156 (= K156), I185 (≠ V185)
8dt1C Crystal structure of a putative d-beta-hydroxybutyrate dehydrogenase from burkholderia cenocepacia j2315 in complex with NAD
36% identity, 98% coverage: 3:252/256 of query aligns to 5:255/259 of 8dt1C
- binding nicotinamide-adenine-dinucleotide: G11 (= G9), S14 (≠ Q12), G15 (= G13), I16 (= I14), D35 (= D33), L36 (≠ Y34), M60 (≠ V58), V62 (= V60), N88 (= N86), A89 (= A87), M139 (≠ A137), Y154 (= Y152), K158 (= K156), P184 (= P182), G185 (= G183), F186 (≠ I184), V187 (= V185), T189 (= T187)
8cxaA Crystal structure of 3-oxoacyl-[acyl-carrier-protein] reductase from mycobacterium smegmatis with bound NAD
37% identity, 99% coverage: 3:256/256 of query aligns to 6:251/251 of 8cxaA
- binding nicotinamide-adenine-dinucleotide: G12 (= G9), Q15 (= Q12), G16 (= G13), I17 (= I14), D36 (= D33), V63 (= V60), N89 (= N86), A91 (≠ G88), S94 (≠ P91), I142 (≠ A137), S143 (≠ C138), S144 (= S139), Y157 (= Y152), K161 (= K156), P187 (= P182), H188 (vs. gap), I190 (= I184), I194 (= I193)
Query Sequence
>BWI76_RS16755 FitnessBrowser__Koxy:BWI76_RS16755
MKKVALVTGAGQGIGKAIALRLVKDGFAVAIADYNDATAQAVADEINRSGGRALAVKVDV
SQRDQVFAAVEQARKGLGGFDVIVNNAGVAPSTPIEEIREDVIDKVYNINVKGVIWGIQA
AVEAFKQEGHGGKIINACSQAGHVGNPELAVYSSSKFAVRGLTQTAARDLAHLGITVNGY
CPGIVKTPMWAEIDRQVSEAAGKPLGYGTQEFAKRITLGRLSEPEDVAACVSYLAGPDSN
YMTGQSLLIDGGMVFN
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SitesBLAST's Database
SitesBLAST's database includes
(1) SwissProt
entries with experimentally-supported functional features;
and (2) protein structures with bound ligands, from the
BioLip database.
by Morgan Price,
Arkin group
Lawrence Berkeley National Laboratory