SitesBLAST

SitesBLAST – Find functional sites

SitesBLAST

Comparing BWI76_RS17835 FitnessBrowser__Koxy:BWI76_RS17835 to proteins with known functional sites using BLASTp with E ≤ 0.001.

Or try Sites on a Tree, PaperBLAST, Conserved Domains, or compare to all protein structures

Found 3 hits to proteins with known functional sites (download)

P94530 Arabinooligosaccharides transport system permease protein AraQ from Bacillus subtilis (strain 168) (see paper)
32% identity, 83% coverage: 48:279/279 of query aligns to 55:281/281 of P94530

query
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P94530

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D180 (= D178) mutation to A: Negative impact in the growth rate in the presence of arabinotriose.

P68183 Maltose/maltodextrin transport system permease protein MalG from Escherichia coli (strain K12) (see 2 papers)
29% identity, 76% coverage: 68:279/279 of query aligns to 84:296/296 of P68183

query
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P68183

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E190 (= E173) mutation E->A,C,K,L: Reduction of transport rate.
A192 (= A175) mutation A->D,S,L: Loss of transport and MalK dissociation from the membrane.
G196 (= G179) mutation to A: No effect.; mutation to P: Loss of transport and MalK dissociation from the membrane.
P209 (= P192) mutation to A: No effect.

P94529 Arabinooligosaccharides transport system permease protein AraP from Bacillus subtilis (strain 168) (see paper)
38% identity, 27% coverage: 141:215/279 of query aligns to 171:254/313 of P94529

query
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P94529

N

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E205 (= E170) mutation to A: Has no effect on the growth rate.
E208 (= E173) mutation to A: Small negative impact in the uptake of arabinotriose. Shows an increase of about 10% in the doubling time.
D213 (= D178) mutation to A: Severely impairs the uptake of arabinotriose. Shows a 3.6-fold increase of the doubling time.

Query Sequence

>BWI76_RS17835 FitnessBrowser__Koxy:BWI76_RS17835
MLSKRWDTAGRWCIYALLLIVFVGPFWGIVATAFSGAPVKPGELLAWPNQFSFENFIFAW
MDIGVWQYLLNSILVVFFGTVLQVSVSALAAYALARKKFRGVALVSLVILSTMMLPEEVI
AIPLYMIINWRLPFIDASLYNSYLGMILPVVGWAFSIFVLTEFMSAIPKELEEAARIDGA
NEWQIFFHVILPLVKPALGTVVTFGFIMIWDQYLLPLIVVNQDSLNTIPVILGTLRTDES
ITPNIFIAITLLAMLPSIIVYLGLQKHFNRGIMSGAVKG

Or try a new SitesBLAST search

SitesBLAST's Database

SitesBLAST's database includes (1) SwissProt entries with experimentally-supported functional features; and (2) protein structures with bound ligands, from the BioLip database.

by Morgan Price, Arkin group
Lawrence Berkeley National Laboratory