SitesBLAST

SitesBLAST – Find functional sites

SitesBLAST

Comparing BWI76_RS19735 BWI76_RS19735 bifunctional PTS fructose transporter subunit IIA/HPr protein to proteins with known functional sites using BLASTp with E ≤ 0.001.

Or try Sites on a Tree, PaperBLAST, Conserved Domains, or compare to all protein structures

Found 19 hits to proteins with known functional sites (download)

P00550 PTS system mannitol-specific EIICBA component; EIICBA-Mtl; EII-Mtl; EC 2.7.1.197 from Escherichia coli (strain K12) (see 2 papers)
46% identity, 38% coverage: 1:143/376 of query aligns to 493:637/637 of P00550

query
sites
P00550

M

L

F

F

Q

K

L

L

S

G

V

A

Q

E

D

N

I

I

H

F

P

L

G

G

Q

R

Q

K

A

A

G

A

N

T

K

K

E

E

A

A

I

I

R

R

Q

F

V

A

A

G

S

E

A

Q

L

L

V

V

S

K

A

G

G

G

N

Y

V

V

A

E

D

P

G

E

Y

Y

V

V

N

Q

G

A

M

M

L

L

A

D

R
|
R

E

E

Q

K

Q

L

T

T

S

P

T

T

F

Y

L

L

G

G

N

E

G

S

I

I

A

A

I

V

P

P

H
|
H

G

G

T

T

T

V

D

E

T

A

R

K

D

D

Q

R

V

V

L

L

K

K

T

T

G

G

V

V

Q

V

V

F

Y

C

Q

Q

F

Y

P

P

Q

E

G

G

V

V

T

R

W

F

G

G

E

E

G

E

Q

E

-

D

T

I

A

A

Y

R

V

L

A

V

I

I

G

G

I

I

A

A

S

R

S

N

D

N

E

E

H

H

L

I

G

Q

L

V

L

I

R

T

Q

S

L

L

T

T

H

N

V

A

L

L

S

D

D

D

D

E

S

S

V

V

A

I

E

E

Q

R

L

L

K

A

S

H

A

T

T

T

T

S

A

V

E

D

E

E

L

V

R

L

A

E

L

L

M

A

G

G

E

R

K

K

R538 (= R46) Stabilizes the transition state in the phosphoryl transfer from HPr to EIIA
H554 (= H62) modified: Phosphohistidine; by HPr

Sites not aligning to the query:

384 modified: Phosphocysteine; by EIIA

1j6tA Complex of enzyme iiamtl and the histidine-containing phosphocarrier protein hpr from escherichia coli nmr, restrained regularized mean structure (see paper)
45% identity, 38% coverage: 1:141/376 of query aligns to 1:143/144 of 1j6tA

query
sites
1j6tA

M

L

F

F

Q

K

L

L

S

G

V

A

Q

E

D

N

I

I

H

F

P

L

G

G

Q

R

Q

K

A

A

G

A

N

T

K

K

E

E

A

A

I

I

R

R

Q

F

V

A

A

G

S

E

A

Q

L

L

V

V

S

K

A

G

G

G

N

Y

V

V

A

E

D

P

G

E

Y

Y

V

V

N

Q

G

A

M

M

L

L

A

D

R
|
R

E

E

Q

K

Q

L

T

T

S

P

T

T

F

Y

L

L

G

G

N

E

G

S

I

I

A

A

I

V

P

P

H
|
H

G

G

T

T

T

V

D

E

T

A

R

K

D

D

Q

R

V

V

L

L

K

K

T

T

G

G

V

V

Q

V

V

F

Y

C

Q

Q

F

Y

P

P

Q

E

G

G

V

V

T

R

W

F

G

G

E

E

G

E

Q

E

-

D

T

I

A

A

Y

R

V

L

A

V

I

I

G

G

I

I

A

A

S

R

S

N

D

N

E

E

H
|
H

L

I

G

Q

L

V

L

I

R

T

Q

S

L

L

T

T

H

N

V

A

L

L

S

D

D

D

D

E

S

S

V

V

A

I

E

E

Q

R

L

L

K

A

S

H

A

T

T

T

T

S

A

V

E

D

E

E

L

V

R

L

A

E

L

L

M

A

G

G

binding phosphite ion: R46 (= R46), H62 (= H62), H108 (= H106)

C0H3V2 Mannitol-specific phosphotransferase enzyme IIA component; EIIA; EIII; PTS system mannitol-specific EIIA component from Bacillus subtilis (strain 168) (see paper)
40% identity, 36% coverage: 4:139/376 of query aligns to 4:139/143 of C0H3V2

query
sites
C0H3V2

L

L

S

A

V

K

Q

E

D

N

I

I

H

K

P

L

G

N

Q

Q

Q

T

A

V

G

S

N

S

K

K

E

E

A

A

I

I

R

K

Q

L

V

A

A

G

S

Q

A

T

L

L

V

I

S

D

A

N

G

G

N

Y

V

V

A

T

D

E

G

D

Y

Y

V

I

N

S

G

K

M

M

L

F

A

E

R

R

E

E

Q

E

Q

T

T

S

S

S

T

T

F

F

L

M

G

G

N

N

G

F

I

I

A

A

I

I

P

P

H

H

G

G

T

T

T

E

D

E

T

A

R

K

D

S

Q

E

V

V

L

L

K

H

T
x
S

G

G

V

I

Q

S

V

I

Y

I

Q

Q

F

I

P

P

Q

E

G

G

V

V

T

E

W

Y

G

G

E

E

G

G

Q

N

T

T

A

A

Y

K

V

V

A

V

I

F

G

G

I

I

A

A

A

G

S

K

S

N

D

N

E

E

H

H

L

L

G

D

L

I

L

L

R

S

Q

N

L

I

T

A

H

I

V

I

L

C

S

S

D

E

S

E

V

N

A

I

E

E

Q

R

L

L

K

I

S

S

A

A

T

K

T

S

A

E

E

E

E

D

L

L

R

I

A

A

L

I

L

F

S74 (≠ T74) modified: Phosphoserine

Q9F166 Phosphocarrier protein HPr from Bacillus thuringiensis subsp. israelensis (see 2 papers)
45% identity, 23% coverage: 290:374/376 of query aligns to 5:87/87 of Q9F166

query
sites
Q9F166

Y

F

V

K

I

V

R

T

N

S

E

D

H

S

G

G

L

I

H
|
H

A

A

R

R

P

P

G

A

T

T

M

L

L

L

V

V

N

N

T

T

I

A

K

S

Q

K

F

F

S

G

S

S

D

D

I

I

T

-

V

-

T

-

N

N

L

L

D

E

G

Y

S

N

G

G

K

K

P

N

A

V

N

N

G

L

R

K

S
|
S

L

I

M

M

K

G

V

V

V

M

A

S

L

L

G

G

V

I

K

Q

G

N

H

A

R

E

L

I

R

K

F

I

T

T

A

A

Q

N

G

G

E

D

D

D

A

A

Q

A

Q

Q

A

A

L

L

D

A

A

A

I

I

G

E

E

E

A

T

I

M

A

K

-

N

A

E

G

G

L

L

G

G

E

E

H14 (= H299) modified: Tele-phosphohistidine; alternate; mutation to A: Not histidine-phosphorylated.
S45 (= S333) modified: Phosphoserine; by HPrK/P; mutation to A: Not serine-phosphorylated. Absence of glucose-induced cry4A expression repression. 60 to 70% reduction in the sporulation efficiency.

P24366 Phosphocarrier protein HPr; Histidine-containing protein from Streptococcus salivarius (see paper)
45% identity, 22% coverage: 287:368/376 of query aligns to 3:81/87 of P24366

query
sites
P24366

S

S

A

K

E

D

Y

F

V

H

I

I

R

V

N

A

E

E

H

T

G

G

L

I

H

H

A

A

R

R

P

P

G

A

T

T

M

L

L

L

V

V

N

Q

T

T

I

A

K

S

Q

K

F

F

S

A

S

S

D

D

I

I

T

T

V

-

T

-

N

-

L

L

D

D

G

Y

S

K

G

G

K

K

P

A

A

V

N

N

G

L

R

K

S
|
S

L

I

M

M

K

G

V

V

V

M

A

S

L

L

G

G

V

V

K

G

K

Q

G

G

H

A

R

D

L

V

R

T

F

I

T

S

A

A

Q

E

G

G

E

A

D

D

A

A

Q

D

A

A

L

I

D

V

A

A

I

I

G

A

E

E

A

T

I

M

S46 (= S333) modified: Phosphoserine; by HPrK/P

Q9CJ83 Phosphocarrier protein HPr; Histidine-containing protein from Lactococcus lactis subsp. lactis (strain IL1403) (Streptococcus lactis) (see paper)
43% identity, 23% coverage: 287:374/376 of query aligns to 3:88/88 of Q9CJ83

query
sites
Q9CJ83

S

S

A

K

E

E

Y

F

V

H

I

I

R

V

N

V

E

E

H

T

G

G

L

I

H

H

A

A

R

R

P

P

G

A

T

T

M

L

L

L

V

V

N

H

T

T

I

A

K

S

Q

K

F

F

S

T

S

S

D

E

I

I

T

T

V

-

T

-

N

-

L

L

D

E

G

Y

S

K

G

G

K

K

P

S

A

V

N

N

G

L

R

K

S
|
S

L

I

M

M

K

G

V

V

V

M

A

S

L

L

G

G

V

V

K

G

K

Q

G

G

H

A

R

D

L

V

R

T

F

I

T

S

A

A

Q

E

G

G

E

A

D

D

A

A

Q

D

A

A

L

I

D

S

A

T

I

I

G

A

E

E

A

T

I

M

A

T

A

K

-

E

G

G

L

L

G

A

E

E

S46 (= S333) modified: Phosphoserine; by HPrK/P; mutation to A: Abolished S-46 phosphorylation.

O69250 Phosphocarrier protein HPr; Histidine-containing protein from Priestia megaterium (Bacillus megaterium) (see 2 papers)
40% identity, 23% coverage: 290:374/376 of query aligns to 6:88/88 of O69250

query
sites
O69250

Y

F

V

T

I

V

R

T

N

A

E

D

H

S

G

G

L

I

H

H

A

A

R

R

P

P

G

A

T

T

M

T

L

L

V

V

N

Q

T

A

I

A

K

S

Q

K

F

F

S

D

S

S

D

D

I

I

T

-

V

-

T

-

N

N

L

L

D

E

G

F

S

N

G

G

K

K

P

T

A

V

N

N

G

L

R

K

S
|
S

L

I

M

M

K

G

V

V

V

M

A

S

L

L

G

G

V

I

K

Q

K

K

G

G

H

A

R

T

L

I

R

T

F

I

T

S

A

A

Q

E

G

G

E

S

D

D

A

E

Q

A

Q

D

A

A

L

L

D

A

A

A

I

L

G

E

E

D

A

T

I

M

A

S

A

K

-

E

G

G

L

L

G

G

E

E

S46 (= S333) modified: Phosphoserine; by HPrK/P

P08877 Phosphocarrier protein HPr; Histidine-containing protein from Bacillus subtilis (strain 168) (see 5 papers)
38% identity, 23% coverage: 290:374/376 of query aligns to 6:88/88 of P08877

query
sites
P08877

Y

F

V

K

I

V

R

T

N

A

E

D

H
x
S

G

G

L

I

H
|
H

A

A

R

R

P

P

G

A

T

T

M

V

L

L

V

V

N

Q

T

T

I

A

K

S

Q

K

F

Y

S

D

S

A

D

D

I

V

T

-

V

-

T

-

N

N

L

L

D

E

G

Y

S

N

G

G

K

K

P

T

A

V

N

N

G

L

R

K

S
|
S

L

I

M

M

K

G

V

V

V

M

A

S

L

L

G

G

V

I

K

A

K

K

G

G

H

A

R

E

L

I

R

T

F

I

T

S

A

A

Q

S

G

G

E

A

D

D

A

E

Q

N

Q

D

A

A

L

L

D

N

A

A

I

L

G

E

E

E

A

T

I

M

A

K

A

S

-

E

G

G

L

L

G

G

E

E

S12 (≠ H296) modified: Phosphoserine
H15 (= H299) active site, Pros-phosphohistidine intermediate; alternate; modified: Tele-phosphohistidine; alternate
S46 (= S333) modified: Phosphoserine; by HPrK/P; mutation to A: No phosphorylation by HPrK/P; abolishes the catabolite repressive effects of glucose on gluconate kinase, glucitol dehydrogenase, and the mannitol-specific catabolic enzymes.

Sites not aligning to the query:

1 modified: Initiator methionine, Removed

Q9WXK8 Phosphocarrier protein HPr; Histidine-containing protein from Streptococcus equinus (Streptococcus bovis) (see paper)
46% identity, 18% coverage: 287:354/376 of query aligns to 3:67/87 of Q9WXK8

query
sites
Q9WXK8

S

S

A

K

E

D

Y

F

V

H

I

I

R

V

N

A

E

E

H

T

G

G

L

I

H

H

A

A

R

R

P

P

G

A

T

T

M

L

L

L

V

V

N

Q

T

T

I

A

K

S

Q

K

F

F

S

A

S

S

D

D

I

I

T

T

V

-

T

-

N

-

L

L

D

D

G

Y

S

K

G

G

K

K

P

A

A

V

N

N

G

L

R

K

S
|
S

L

I

M

M

K

G

V

V

V

M

A

S

L

L

G

G

V

V

K

G

K

Q

G

G

H

A

R

D

L

V

R

T

F

I

T

S

A

A

Q

E

G

G

S46 (= S333) mutation to A: No phosphorylation by HPrK/P.

1pohA The 2.0 angstroms resolution structure of escherichia coli histidine- containing phosphocarrier protein hpr: a redetermination (see paper)
36% identity, 23% coverage: 285:369/376 of query aligns to 1:82/85 of 1pohA

query
sites
1pohA

V

M

L

F

S

Q

A

Q

E

E

Y

V

V

T

I

I

R

T

N

A

E

P

H

N

G

G

L

L

H

H

A

T

R

R

P

P

G

A

T

A

M

Q

L

F

V

V

N

K

T

E

I

A

K

K

Q

G

F

F

S

T

S

S

D

E

I

I

T

T

V

V

T

T

N

S

L

-

D

-

G

-

S
x
N

G

G

K
|
K

P

S

A

A

N

S

G

A

R

K

S

S

L

L

M

F

K

K

V

L

V

Q

A

T

L

L

G

G

V

L

K

T

K

Q

G

G

H

T

R

V

L

V

R

T

F

I

T

S

A

A

Q

E

G

G

E

E

D

D

A

E

Q

Q

Q

K

A

A

L

V

D

E

A

H

I

L

G

V

E

K

A

L

I

M

A

A

binding sulfate ion: N38 (≠ S325), K40 (= K327)

1j6tB Complex of enzyme iiamtl and the histidine-containing phosphocarrier protein hpr from escherichia coli nmr, restrained regularized mean structure (see paper)
36% identity, 23% coverage: 285:369/376 of query aligns to 1:82/85 of 1j6tB

query
sites
1j6tB

V

M

L

F

S

Q

A

Q

E

E

Y

V

V

T

I

I

R

T

N

A

E

P

H

N

G

G

L

L

H
|
H

A
x
T

R

R

P

P

G

A

T

A

M

Q

L

F

V

V

N

K

T

E

I

A

K

K

Q

G

F

F

S

T

S

S

D

E

I

I

T

T

V

V

T

T

N

S

L

-

D

-

G

-

S

N

G

G

K

K

P

S

A

A

N

S

G

A

R

K

S

S

L

L

M

F

K

K

V

L

V

Q

A

T

L

L

G

G

V

L

K

T

K

Q

G

G

H

T

R

V

L

V

R

T

F

I

T

S

A

A

Q

E

G

G

E

E

D

D

A

E

Q

Q

Q

K

A

A

L

V

D

E

A

H

I

L

G

V

E

K

A

L

I

M

A

A

binding phosphite ion: H15 (= H299), T16 (≠ A300)

P0AA04 Phosphocarrier protein HPr; Histidine-containing protein from Escherichia coli (strain K12) (see paper)
36% identity, 23% coverage: 285:369/376 of query aligns to 1:82/85 of P0AA04

query
sites
P0AA04

V

M

L

F

S

Q

A

Q

E

E

Y

V

V

T

I

I

R

T

N

A

E

P

H

N

G

G

L

L

H
|
H

A

T

R

R

P

P

G

A

T

A

M

Q

L

F

V

V

N

K

T

E

I

A

K

K

Q

G

F

F

S

T

S

S

D

E

I

I

T

T

V

V

T

T

N

S

L

-

D

-

G

-

S

N

G

G

K

K

P

S

A

A

N

S

G

A

R

K

S

S

L

L

M

F

K

K

V

L

V

Q

A

T

L

L

G

G

V

L

K

T

K

Q

G

G

H

T

R

V

L

V

R

T

F

I

T

S

A

A

Q

E

G

G

E

E

D

D

A

E

Q

Q

Q

K

A

A

L

V

D

E

A

H

I

L

G

V

E

K

A

L

I

M

A

A

H15 (= H299) active site, Pros-phosphohistidine intermediate

O06976 HPr-like protein Crh; Catabolite repression HPr from Bacillus subtilis (strain 168) (see 3 papers)
36% identity, 20% coverage: 292:365/376 of query aligns to 8:78/85 of O06976

query
sites
O06976

I

V

R

R

N

L

E

K

H

T

G

G

L

L

H
x
Q

A

A

R

R

P

P

G

A

T

A

M

L

L

F

V

V

N

Q

T

E

I

A

K

N

Q

R

F

F

S

T

S

S

D

D

I

V

T

F

V

-

T

-

N

-

L

L

D

E

G

K

S

D

G

G

K

K

P

K

A

V

N

N

G

A

R

K

S
|
S

L

I

M

M

K

G

V

L

V

M

A

S

L

L

G

A

V

V

K

S

K

T

G

G

H

T

R

E

L

V

R

T

F

L

T

I

A

A

Q

Q

G

G

E

E

D

D

A

E

Q

Q

Q

E

A

A

L

L

D

E

A

K

I

L

G

A

Q15 (≠ H299) mutation to H: Cannot fulfill its catalytic role within PTS transport. Does not affect dimerization.
S46 (= S333) modified: Phosphoserine; by HPrK/P

P37349 PEP-dependent dihydroxyacetone kinase, phosphoryl donor subunit DhaM; Dihydroxyacetone kinase subunit M; EC 2.7.1.121 from Escherichia coli (strain K12) (see paper)
34% identity, 25% coverage: 280:374/376 of query aligns to 150:241/472 of P37349

query
sites
P37349

A

A

I

Y

A

D

E

E

D

E

V

A

L

R

S

S

A

L

E

A

Y

V

V

V

I

I

R

K

N

N

E

R

H

N

G

G

L

L

H
|
H

A

V

R

R

P

P

G

A

T

S

M

R

L

L

V

V

N

Y

T

T

I

L

K

S

Q

T

F

F

S

N

S

A

D

D

I

M

T

L

V

-

T

-

N

-

L

L

D

E

G

K

S

N

G

G

K

K

P

C

A

V

N

T

G

P

R

E

S

S

L

I

M

N

K

Q

V

I

V

A

A

L

L

L

G

Q

V

V

K

R

K

Y

G

N

H

D

R

T

L

L

R

R

F

L

T

I

A

A

Q

K

G

G

E

P

D

E

A

A

Q

E

A

A

L

L

D

I

A

A

I

F

G

R

E

Q

A

L

I

A

A

E

A

D

G

N

L

F

G

G

E

E

H169 (= H299) mutation to A: Loss of phosphotransfer activity.

Sites not aligning to the query:

9 H→A: Loss of phosphotransfer activity.
430 H→A: Loss of phosphotransfer activity.

O31645 PTS system mannose-specific EIIBCA component; EIIBCA-Man; EII-Man; EC 2.7.1.191 from Bacillus subtilis (strain 168) (see 2 papers)
35% identity, 27% coverage: 38:139/376 of query aligns to 542:646/650 of O31645

query
sites
O31645

G

G

Y

F

V

K

N

Q

G

A

M

I

L

L

A

N

R

R

E

E

Q

Q

T

G

S

T

T

T

F

A

L

I

G

G

N

M

G

N

I

I

A

A

I

I

P

P

H

H

G

G

T

K

T

S

D

E

-

A

T

V

R

R

D

E

Q

P

V

S

L

V

K

A

T

F

G

G

V

I

Q

K

V

R

Y

-

Q

-

F

-

P

-

Q

S

G

G

V

V

T

D

W

W

G

N

-

S

-

L

E

D

G

G

Q

S

T

E

A

A

Y

K

V

L

A

I

I

F

G

M

I

I

A

A

A

V

S

P

S

K

D

E

-

S

-

G

-

N

E

Q

H

H

L

L

G

K

L

I

L

L

R

Q

Q

M

L

L

T

S

H

R

V

K

L

L

S

M

D

D

S

N

V

Y

A

R

E

E

Q

R

L

L

K

L

S

S

A

V

T

Q

T

T

A

T

E

E

L

A

R

Y

A

K

L

L

Sites not aligning to the query:

9 C→A: Appears to be unable to inactivate ManR, since ManR does not show any decrease in its DNA-binding activity in the presence of this mutant.
365 modified: Phosphoserine

O50515 Phosphocarrier protein HPr; Histidine-containing protein from Streptomyces coelicolor (strain ATCC BAA-471 / A3(2) / M145) (see paper)
33% identity, 21% coverage: 296:374/376 of query aligns to 12:88/93 of O50515

query
sites
O50515

H

E

G

G

L

L

H
|
H

A

A

R

R

P

P

G

A

T

S

M

I

L

F

V

V

N

R

T

A

I

A

K

T

Q

A

F

T

S

G

S

V

D

P

I

V

T

T

V

I

T

A

N

K

L

A

D

D

G

G

S

S

G

-

K

-

P

P

A

V

N

N

G

A

R

A

S

S

L

M

M

L

K

A

V

V

V

L

A

G

L

L

G

G

V

A

K

Q

K

G

G

G

H

E

R

E

L

I

R

V

F

L

T

A

A

S

Q

D

G

A

E

E

D

G

A

A

Q

E

Q

A

A

A

L

L

D

E

A

R

I

L

G

A

E

K

A

L

I

V

A

A

A

E

G

G

L

L

G

E

E

E

H15 (= H299) mutation to A: Loss of phosphorylation.

Sites not aligning to the query:

1 modified: Initiator methionine, Removed

Q84F84 Phosphocarrier protein HPr; Histidine-containing protein from Lysinibacillus sphaericus (Bacillus sphaericus) (see paper)
33% identity, 20% coverage: 289:364/376 of query aligns to 5:77/88 of Q84F84

query
sites
Q84F84

E

Q

Y

F

V

T

I

V

R

I

N

D

E

P

H

L

G

G

L

I

H
|
H

A

A

R

R

P

P

G

A

T

S

M

Q

L

L

V

V

N

A

T

K

I

A

K

T

Q

P

F

F

S

A

S

S

D

A

I

I

T

E

V

V

T

R

N

T

L

E

D

E

G

-

S

-

G

-

K

K

P

A

A

A

N

N

G

L

R

K

S
|
S

L

I

M

L

K

G

V

V

V

M

A

G

L

L

G

A

V

L

K

K

K

Q

G

G

H

S

R

Q

L

F

R

T

F

L

T

Y

A

V

Q

E

G

G

E

E

D

D

A

E

Q

D

Q

Q

A

A

L

F

D

E

A

A

I

L

H15 (= H299) active site, Pros-phosphohistidine intermediate
S46 (= S333) modified: Phosphoserine; by HPrK/P; mutation to Q: No phosphorylation by HPrK/P.

O31644 Transcriptional regulator ManR; Mannose operon transcriptional activator; EC 2.7.1.191 from Bacillus subtilis (strain 168) (see paper)
24% identity, 31% coverage: 13:130/376 of query aligns to 521:637/648 of O31644

query
sites
O31644

Q

Q

Q

E

A

A

G

E

N

H

K

R

E

Y

E

K

A

L

I

I

R

E

Q

Q

V

L

A

A

S

T

A

A

L

L

V

F

S

E

A

K

G

G

N

Y

V

V

A

D

D

K

G

D

Y

Y

V

A

N

V

G

H

M

A

L

V

A

M

R

R

E

E

Q

K

Q

M

T

S

S

A

T

T

F

N

L

I

G

G

N

S

G

G

I

I

A

A

I

I

P

P

H
|
H

G

A

T

N

T

A

D

K

T

F

R

I

D

K

Q

Q

V

-

L

-

K

-

T

S

G

A

V

I

Q

A

V

I

Y

A

Q

T

F

L

P

K

Q

E

G

P

V

L

T

E

W

W

G

G

E

N

G

E

Q

K

T

V

A

S

Y

L

V

V

A

F

I

M

G

L

I

A

A

V

A

K

S

H

S

E

D

D

E

Q

H

T

L

M

G

T

-

K

-

Q

L

L

L

F

R

S

Q

E

L

L

T

S

H

Y

V

L

L

S

S

E

D

Q

D

P

S

A

V

F

A

V

E

Q

Q

K

L

L

K

T

S

K

A

E

T

T

H570 (= H62) mutation to A: No reduction in DNA-binding activity can be observed in the presence of EIIBA-Man. Leads to a nearly constitutive ManR activity.

Sites not aligning to the query:

222 H→A: Reduced DNA binding.
281 H→A: Reduced DNA binding.
334 H→A: Loss of DNA binding.
393 H→A: Loss of DNA binding.
415 C→A: No effect on DNA binding. Results in a weak constitutive ManR activity.

P75061 Phosphocarrier protein HPr; Histidine-containing protein from Mycoplasma pneumoniae (strain ATCC 29342 / M129 / Subtype 1) (Mycoplasmoides pneumoniae) (see paper)
29% identity, 20% coverage: 289:364/376 of query aligns to 5:78/88 of P75061

query
sites
P75061

E

Q

Y

V

V

V

I

V

R

K

N

D

E

P

H

V

G

G

L

I

H

H

A

A

R

R

P

P

G

A

T

S

M

I

L

I

V

A

N

G

T

E

I

A

K

N

Q

K

F

F

S

K

S

S

D

E

I

L

T

K

V

L

T

-

N

-

L

V

D

S

G

P

S

S

G

G

K

V

P

E

A

G

N

N

G

I

R

K

S
|
S

L

I

M

I

K

N

V

L

V

M

A

S

L

L

G

G

V

I

K

K

K

Q

G

N

H

D

R

H

L

I

R

T

F

I

T

K

A

A

Q

E

G

G

E

T

D

D

A

E

Q

E

A

A

L

L

D

N

A

A

I

I

S47 (= S333) modified: Phosphoserine; by HPrK/P

Query Sequence

>BWI76_RS19735 BWI76_RS19735 bifunctional PTS fructose transporter subunit IIA/HPr protein
MFQLSVQDIHPGQQAGNKEEAIRQVASALVSAGNVADGYVNGMLAREQQTSTFLGNGIAI
PHGTTDTRDQVLKTGVQVYQFPQGVTWGEGQTAYVAIGIAASSDEHLGLLRQLTHVLSDD
SVAEQLKSATTAEELRALLMGEKQSEALKLDNETLTLDVAASDLLTLQALNAARLKEAGA
VDASFVSRTINDKPLNLGQGIWLNDSTEGNLRSAVAVSRAANAFTSDEQPVSLLITVAMA
DDQPTAVLNRLSNLLLDKKADRLLKADGATLLALLTSDDAIAEDVLSAEYVIRNEHGLHA
RPGTMLVNTIKQFSSDITVTNLDGSGKPANGRSLMKVVALGVKKGHRLRFTAQGEDAQQA
LDAIGEAIAAGLGEGA

Or try a new SitesBLAST search

SitesBLAST's Database

SitesBLAST's database includes (1) SwissProt entries with experimentally-supported functional features; and (2) protein structures with bound ligands, from the BioLip database.

by Morgan Price, Arkin group
Lawrence Berkeley National Laboratory