SitesBLAST

SitesBLAST – Find functional sites

SitesBLAST

Comparing BWI76_RS19965 BWI76_RS19965 sn-glycerol-3-phosphate dehydrogenase subunit A to proteins with known functional sites using BLASTp with E ≤ 0.001.

Or try Sites on a Tree, PaperBLAST, Conserved Domains, or compare to all protein structures

Found 20 (the maximum) hits to proteins with known functional sites (download)

Q9SS48 Glycerol-3-phosphate dehydrogenase SDP6, mitochondrial; Protein SUGAR-DEPENDENT 6; EC 1.1.5.3 from Arabidopsis thaliana (Mouse-ear cress) (see paper)
29% identity, 72% coverage: 8:394/540 of query aligns to 75:484/629 of Q9SS48

query
sites
Q9SS48

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S86 (≠ A19) mutation to F: In spd6-2; loss of 90% of activity.

Sites not aligning to the query:

546 E→G: In spd6-1; loss of 90% of activity.

2rgoA Structure of alpha-glycerophosphate oxidase from streptococcus sp.: A template for the mitochondrial alpha-glycerophosphate dehydrogenase (see paper)
28% identity, 62% coverage: 29:363/540 of query aligns to 41:393/557 of 2rgoA

query
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2rgoA

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binding flavin-adenine dinucleotide: E48 (= E36), M49 (≠ R37), Q50 (≠ Y38), T56 (≠ A44), S57 (≠ T45), A197 (≠ V172), S232 (≠ A207), G233 (= G208), W235 (= W210), G257 (= G229), G344 (= G311), L345 (≠ V312), Y357 (≠ P322), K382 (= K352), I383 (≠ L353), T384 (≠ M354)

Sites not aligning to the query:

binding flavin-adenine dinucleotide: 25, 28, 29

2rgoB Structure of alpha-glycerophosphate oxidase from streptococcus sp.: A template for the mitochondrial alpha-glycerophosphate dehydrogenase (see paper)
27% identity, 62% coverage: 29:363/540 of query aligns to 39:385/530 of 2rgoB

query
sites
2rgoB

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binding flavin-adenine dinucleotide: E46 (= E36), M47 (≠ R37), Q48 (≠ Y38), T54 (≠ A44), S55 (≠ T45), S58 (≠ N48), T59 (≠ H49), A193 (≠ V172), S227 (≠ A207), G228 (= G208), W230 (= W210), L338 (≠ V312), R339 (= R313), Y350 (≠ V327), K374 (= K352), I375 (≠ L353), T376 (≠ M354)

Sites not aligning to the query:

binding flavin-adenine dinucleotide: 25, 26, 27

3da1A X-ray structure of the glycerol-3-phosphate dehydrogenase from bacillus halodurans complexed with fad. Northeast structural genomics consortium target bhr167.
29% identity, 71% coverage: 5:386/540 of query aligns to 17:354/496 of 3da1A

query
sites
3da1A

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W

G

M

L

L

F

L

I

-

T

-

L

-

P

-

E

-

D

-

P

P

A

I

Y

F

Q

K

H

R

T

Y

F

M

I

L

T

N

A

E

C

K

Q

Q

Q

T

A

-

G

-

I

L

E

E

A

K

Q

E

P

P

L

L

T

L

P

R

A

K

E

E

T

N

L

L

R

K

L

-

E

-

P

-

A

-

V

-

N

-

P

-

A

-

L

-

L

G

G

G

A

G

V

I

Q

Y

V

V

P

E

D

Y

G

R

T

T

V

D

D

D

P

A

F

-

R

R

L

L

T

T

A

L

A

E

N

I

M

M

L

K

D

E

A

A

R

V

E

A

H

R

G

G

A

A

T

V

I

A

L

L

T

N

G

Y

C

M

E

K

V
|
V

T

E

G

S

L

F

L

I

R

Y

E

D

G

Q

D

G

R

K

V

V

Q

V

G

G

V

V

A

V

L

A

Y

K

D

D

R

R

Q

L

R

T

R

D

E

T

P

T

L

H

S

T

L

I

R

Y

A

A

Q

K

V

K

V

V

N

N

A

A

G
|
G

I

P

W
|
W

G

V

Q

D

R

T

I
x
L

A

R

E

E

Y

K

A

D

D

R

L

S

R

K

I

H

A

G

M

K

F

Y

-

L

-

K

P

L

A

S

K

K

G
|
G

S

V

L

H

L

L

I

V

L

V

D

D

H

Q

R

S

-

R

-

F

-

P

I

L

N

R

R

Q

Q

A

V

V

I

Y

N

F

R

D

C

T

R

E

K

S

P

D

A

G

D

R

A

M

D

I

I

F

L

A

V

I

P

P

G

R

D

E

T

G

I

K

S

T

L

Y

I

I

G

G

T
|
T

T

T

S

D

M

T

H

F

I

Y

P

D

Y

K

D

D

E

-

I

I

D

A

D

S

N

P

R

R

V

M

T

T

A

V

G

E

E

D

V

R

D

D

T

Y

L

I

L

L

R

A

E

A

G

A

E

N

K

Y

L

M

A

F

P

P

I

S

M

L

G

R

R

L

T

T

-

A

-

D

R

D

I

V

L

E

R

S

A

S

Y

W

S

A

G
|
G

V

L

R
|
R

P

P

L

L

V

I

A

H

S

E

D

D

D

E

D

-

P

-

S

-

G

-

R

-

S

-

V

-

S

-

R

-

G

-

I

I

V

F

L

F

L

S

D

D

H

-

A

-

Q

-

R

-

D

-

G

-

M

-

E

S

G

G

F

L

I

I

T

S

I

I

T

A

G

G

G
|
G

K
|
K

L
|
L

M

T

T

G

Y

Y

R

R

L

K

M

M

A

A

E

E

W

R

A

T

T

V

D

D

A

A

V

V

C

A

R

Q

K

G

L

L

G

N

N

V

T

N

A

E

P

P

C

C

T

T

A

A

D

A

A

I

P

R

L

L

P

S

G

G

binding flavin-adenine dinucleotide: I24 (= I12), G27 (= G15), I28 (≠ A16), T29 (= T17), E48 (= E36), M49 (≠ R37), G55 (= G43), S57 (≠ T45), S60 (≠ N48), T61 (≠ H49), L63 (= L51), H65 (= H53), V151 (= V172), G187 (= G208), W189 (= W210), L193 (≠ I214), G210 (= G229), T250 (= T266), G296 (= G311), R298 (= R313), G319 (= G351), K320 (= K352), L321 (= L353)

2qcuB Crystal structure of glycerol-3-phosphate dehydrogenase from escherichia coli (see paper)
25% identity, 81% coverage: 30:465/540 of query aligns to 27:475/501 of 2qcuB

query
sites
2qcuB

L

L

K

S

A

V

A

L

L

M

V

L

E
|
E

R
x
A

Y

Q

D

D

I

L

A

A

T
x
C

G

A

A
x
T

T
x
S

G

S

R

A

N
x
S

H
x
S

G

K

L
|
L

L

I

H
|
H

S

G

G

G

A

L

R
|
R

Y
|
Y

-

L

A

E

V

H

T

Y

D

E

S

F

E

R

S

L

A

V

R

S

E

E

C

A

I

L

S

A

E

E

N

R

R

E

I

V

L

L

R

L

R

K

I

M

A

A

R

P

H

H

C

I

I

A

E

F

P

P

T

M

S

R

G

F

L

R

F

L

I

P

T

H

L

R

P

P

E

H

D

L

D

R

P

P

A

A

Y

W

Q

M

H

-

T

I

F

R

I

I

T

G

A

L

C

F

Q

M

Q

Y

A

D

G

H

I

L

E

G

A

K

Q

R

P

T

L

S

T

L

P

P

A

G

E

S

T

T

-

G

L

L

R

R

L

F

-

G

-

A

E

N

P

S

A

V

V

L

N

K

P

P

A

E

L

I

L

K

G

R

A

G

V

F

Q

E

V

Y

P

S

D

D

G

C

T

W

V

V

D

D

P

D

F

A

R

R

L

L

T

V

A

L

A

A

N

N

M

A

L

Q

D

M

A

V

R

V

E

R

H

K

G

G

A

G

T

E

I

V

L

L

T

T

G

R

C

T

E

R

V
x
A

T

T

G

S

L

A

L

R

R

R

E

E

G

-

D

N

R

G

V

L

Q

W

G

I

V

V

A

E

L

A

Y

E

D

D

R

I

Q

D

R

T

R

G

E

K

P

K

L

Y

S

S

L

W

R

Q

A

A

Q

R

V

G

V

L

V

V

N

N

A

A

A
x
T

G

G

I

P

W
|
W

G

-

Q

-

R

-

I

V

A

K

E

Q

Y

F

A

F

D

D

L

D

R

G

I

M

A

H

M

L

F

P

P

S

A

P

K

Y

G

G

S

I

L

R

L

L

I

I

L

K

D
x
G

H

S

R

H

I

I

N

-

R

-

Q

-

V

V

I

V

N

P

R

R

C

V

R

H

K

T

P

Q

A

K

D

Q

A

A

D

Y

I

I

L

L

-

Q

-

N

-

E

-

D

-

K

-

R

-

I

-

V

-

F

-

V

V

I

P

P

G

W

-

M

D

D

T

E

I

F

S

S

L

I

I

I

G

G

T

T

S

D

M

V

H

E

I

Y

P

K

Y

G

D

D

E

P

I

-

D

K

D

A

N

V

R

K

V

I

T

E

A

E

G

S

E

E

V

I

D

N

T

Y

L

L

R

N

-

V

E

Y

G

N

E

T

K

H

L

F

A

K

P

K

I

Q

M

L

G

S

R

R

T

D

R

D

I

I

L

V

R

W

A

T

Y

Y

S

S

G

G

V

V

R
|
R

P

P

L

L

V

-

A

C

S

D

D

D

D

E

D

S

P

D

S

S

G

P

R

Q

S

A

V

I

S

T

R
|
R

G

D

I

Y

V

T

L

L

L

D

D

I

H

H

A

D

Q

E

R

-

D

N

G

G

M

K

E

A

G

P

F

L

I

L

T

S

I

V

T

F

G

G

G
|
G

K
|
K

L
|
L

M
x
T

T

T

Y

Y

R

R

L

K

M

L

A

A

E

E

W

H

A

A

T

L

D

E

A

K

V

L

C

T

R

P

K

Y

L

Y

G

Q

N

G

T

I

A

G

P

P

C

A

T

W

T

T

A

K

D

E

A

S

P

V

L

L

P

P

G

G

S

G

-

A

-

I

-

E

-

G

-

D

-

R

Q

D

E

D

P

Y

T

A

E

A

T

R

T

L

L

R

R

R

K

R

I

Y

I

P

A

F

L

L

P

T

A

E

S

S

L

L

R

A

G

R

S

H

A

Y

I

A

Y

R

R

T

H

Y

G

G

D

S

R

N

T

S

P

E

A

L

W

L

L

L

G

G

D

N

S

A

R

G

Q

T

H

V

R

S

S

D

L

L

V

G

C

E

E

D

C

F

-

G

E

H

A

E

V

F

T

Y

A

E

G

A

E

E

V

L

Q

K

Y

Y

A

L

V

V

E

D

N

H

L

E

A

W

V

V

K

R

N

R

L

A

L

D

D

D

-

A

L

L

R

W

R

R

T

T

R

K

V

Q

G

G

M

M

binding flavin-adenine dinucleotide: E33 (= E36), A34 (≠ R37), C39 (≠ T42), T41 (≠ A44), S42 (≠ T45), S45 (≠ N48), S46 (≠ H49), L48 (= L51), H50 (= H53), A172 (≠ V172), T206 (≠ A207), W209 (= W210), G231 (≠ D235), R317 (= R313), G353 (= G351), K354 (= K352), L355 (= L353), T356 (≠ M354)
binding phosphate ion: R54 (= R57), Y55 (= Y58), R317 (= R313), R332 (= R329)

Sites not aligning to the query:

binding flavin-adenine dinucleotide: 9, 12, 13, 14

2r46A Crystal structure of escherichia coli glycerol-3-phosphate dehydrogenase in complex with 2-phosphopyruvic acid. (see paper)
25% identity, 81% coverage: 30:465/540 of query aligns to 27:475/495 of 2r46A

query
sites
2r46A

L

L

K

S

A

V

A

L

L

M

V
x
L

E
|
E

R
x
A

Y

Q

D

D

I

L

A

A

T

C

G

A

A
x
T

T
x
S

G

S

R
x
A

N
x
S

H
x
S

G

K

L
|
L

L

I

H

H

S

G

G

G

A

L

R
|
R

Y
|
Y

-

L

A

E

V

H

T

Y

D

E

S

F

E

R

S

L

A

V

R

S

E

E

C

A

I

L

S

A

E

E

N

R

R

E

I

V

L

L

R

L

R

K

I

M

A

A

R

P

H

H

C

I

I

A

E

F

P

P

T

M

S

R

G

F

L

R

F

L

I

P

T

H

L

R

P

P

E

H

D

L

D

R

P

P

A

A

Y

W

Q

M

H

-

T

I

F

R

I

I

T

G

A

L

C

F

Q

M

Q

Y

A

D

G

H

I

L

E

G

A

K

Q

R

P

T

L

S

T

L

P

P

A

G

E

S

T

T

-

G

L

L

R

R

L

F

-

G

-

A

E

N

P

S

A

V

V

L

N

K

P

P

A

E

L

I

L

K

G

R

A

G

V

F

Q

E

V

Y

P

S

D

D

G

C

T

W

V

V

D

D

P

D

F

A

R

R

L

L

T

V

A

L

A

A

N

N

M

A

L
x
Q

D

M

A

V

R

V

E

R

H

K

G

G

A

G

T

E

I

V

L

L

T

T

G

R

C

T

E

R

V
x
A

T

T

G

S

L

A

L

R

R

R

E

E

G

-

D

N

R

G

V

L

Q

W

G

I

V

V

A

E

L

A

Y

E

D

D

R

I

Q

D

R

T

R

G

E

K

P

K

L

Y

S

S

L

W

R

Q

A

A

Q

R

V

G

V

L

V

V

N

N

A

A

A
x
T

G

G

I

P

W

W

G

-

Q

-

R

-

I

V

A

K

E

Q

Y

F

A

F

D

D

L

D

R

G

I

M

A

H

M

L

F

P

P

S

A

P

K

Y

G

G

S

I

L

R

L

L

I

I

L

K

D
x
G

H

S

R

H

I

I

N

-

R

-

Q

-

V

V

I

V

N

P

R

R

C

V

R

H

K

T

P

Q

A

K

D

Q

A

A

D

Y

I

I

L

L

-

Q

-

N

-

E

-

D

-

K

-
x
R

-

I

-

V

-

F

-

V

V

I

P

P

G

W

-

M

D

D

T

E

I

F

S

S

L

I

I

I

G

G

T
|
T

T

T

S
x
D

M

V

H

E

I

Y

P

K

Y

G

D

D

E

P

I

-

D

K

D

A

N

V

R

K

V

I

T

E

A

E

G

S

E

E

V

I

D

N

T

Y

L

L

R

N

-

V

E

Y

G

N

E

T

K

H

L

F

A

K

P

K

I

Q

M

L

G

S

R

R

T

D

R

D

I

I

L

V

R

W

A

T

Y

Y

S

S

G

G

V

V

R
|
R

P

P

L

L

V

-

A

C

S

D

D

D

D

E

D

S

P

D

S

S

G

P

R

Q

S

A

V

I

S

T

R
|
R

G

D

I

Y

V

T

L

L

L

D

D

I

H

H

A

D

Q

E

R

-

D

N

G

G

M

K

E

A

G

P

F

L

I

L

T

S

I

V

T

F

G

G

G
|
G

K
|
K

L
|
L

M
x
T

T

T

Y

Y

R

R

L

K

M

L

A

A

E

E

W

H

A

A

T

L

D

E

A

K

V

L

C

T

R

P

K

Y

L

Y

G

Q

N

G

T

I

A

G

P

P

C

A

T

W

T

T

A

K

D

E

A

S

P

V

L

L

P

P

G

G

S

G

-

A

-

I

-

E

-

G

-

D

-

R

Q

D

E

D

P

Y

T

A

E

A

T

R

T

L

L

R

R

R

K

R

I

Y

I

P

A

F

L

L

P

T

A

E

S

S

L

L

R

A

G

R

S

H

A

Y

I

A

Y

R

R

T

H

Y

G

G

D

S

R

N

T

S

P

E

A

L

W

L

L

L

G

G

D

N

S

A

R

G

Q

T

H

V

R

S

S

D

L

L

V

G

C

E

E

D

C

F

-

G

E

H

A

E

V

F

T

Y

A

E

G

A

E

E

V

L

Q

K

Y

Y

A

L

V
|
V

E
x
D

N
x
H

L

E

A
x
W

V

V

K

R

N

R

L

A

L

D

D

D

-

A

L

L

R

W

R

R

T

T

R

K

V

Q

G

G

M

M

binding flavin-adenine dinucleotide: L32 (≠ V35), E33 (= E36), A34 (≠ R37), T41 (≠ A44), S42 (≠ T45), A44 (≠ R47), S45 (≠ N48), S46 (≠ H49), L48 (= L51), A172 (≠ V172), T206 (≠ A207), G231 (≠ D235), R317 (= R313), G353 (= G351), K354 (= K352), L355 (= L353), T356 (≠ M354)
binding phosphoenolpyruvate: R54 (= R57), Y55 (= Y58), R254 (vs. gap), T270 (= T266), D272 (≠ S268), R317 (= R313), R332 (= R329)
binding n-(tris(hydroxymethyl)methyl)-3-aminopropanesulfonic acid: Q157 (≠ L157), V455 (= V446), D456 (≠ E447), H457 (≠ N448), W459 (≠ A450)

Sites not aligning to the query:

binding flavin-adenine dinucleotide: 9, 12, 13, 14

2r45A Crystal structure of escherichia coli glycerol-3-phosphate dehydrogenase in complex with 2-phospho-d-glyceric acid (see paper)
25% identity, 81% coverage: 30:465/540 of query aligns to 27:475/495 of 2r45A

query
sites
2r45A

L

L

K

S

A

V

A

L

L

M

V

L

E
|
E

R
x
A

Y

Q

D

D

I

L

A

A

T
x
C

G
x
A

A
x
T

T
x
S

G

S

R
x
A

N
x
S

H
x
S

G

K

L
|
L

L

I

H

H

S

G

G

G

A

L

R
|
R

Y
|
Y

-

L

A

E

V

H

T

Y

D

E

S

F

E

R

S

L

A

V

R

S

E

E

C

A

I

L

S

A

E

E

N

R

R

E

I

V

L

L

R

L

R

K

I

M

A

A

R

P

H

H

C

I

I

A

E

F

P

P

T

M

S

R

G

F

L

R

F

L

I

P

T

H

L

R

P

P

E

H

D

L

D

R

P

P

A

A

Y

W

Q

M

H

-

T

I

F

R

I

I

T

G

A

L

C

F

Q

M

Q

Y

A

D

G

H

I

L

E

G

A

K

Q

R

P

T

L

S

T

L

P

P

A

G

E

S

T

T

-

G

L

L

R

R

L

F

-

G

-

A

E

N

P

S

A

V

V

L

N

K

P

P

A

E

L

I

L

K

G

R

A

G

V

F

Q

E

V

Y

P

S

D

D

G

C

T

W

V

V

D

D

P

D

F

A

R

R

L

L

T

V

A

L

A

A

N

N

M

A

L
x
Q

D

M

A

V

R
x
V

E

R

H

K

G

G

A

G

T

E

I

V

L

L

T

T

G

R

C

T

E

R

V
x
A

T

T

G

S

L

A

L

R

R

R

E

E

G

-

D

N

R

G

V

L

Q

W

G

I

V

V

A

E

L

A

Y

E

D

D

R

I

Q

D

R

T

R

G

E

K

P

K

L

Y

S

S

L

W

R

Q

A

A

Q

R

V

G

V

L

V

V

N

N

A

A

A
x
T

G
|
G

I

P

W
|
W

G

-

Q

-

R

-

I

V

A

K

E
x
Q

Y

F

A

F

D

D

L

D

R

G

I

M

A

H

M

L

F

P

P

S

A

P

K

Y

G

G

S

I

L

R

L

L

I

I

L

K

D
x
G

H

S

R

H

I

I

N

-

R

-

Q

-

V

V

I

V

N

P

R

R

C

V

R

H

K

T

P

Q

A

K

D

Q

A

A

D

Y

I

I

L

L

-

Q

-

N

-

E

-

D

-

K

-
x
R

-
x
I

-

V

-

F

-

V

V

I

P

P

G

W

-

M

D

D

T

E

I

F

S

S

L

I

I

I

G

G

T
|
T

T

T

S

D

M

V

H

E

I

Y

P

K

Y
x
G

D

D

E
x
P

I

-

D

K

D

A

N

V

R

K

V

I

T

E

A

E

G

S

E

E

V

I

D

N

T

Y

L

L

R

N

-

V

E

Y

G

N

E

T

K

H

L

F

A

K

P

K

I

Q

M

L

G

S

R

R

T

D

R

D

I

I

L
x
V

R

W

A

T

Y

Y

S

S

G

G

V

V

R
|
R

P

P

L

L

V

-

A

C

S

D

D

D

D

E

D

S

P

D

S

S

G

P

R

Q

S

A

V

I

S

T

R
|
R

G

D

I

Y

V

T

L

L

L

D

D

I

H

H

A

D

Q

E

R

-

D

N

G

G

M

K

E

A

G

P

F

L

I

L

T

S

I

V

T

F

G

G

G
|
G

K
|
K

L
|
L

M
x
T

T

T

Y

Y

R

R

L

K

M

L

A

A

E

E

W

H

A

A

T

L

D

E

A

K

V

L

C

T

R

P

K

Y

L

Y

G

Q

N

G

T

I

A

G

P

P

C

A

T

W

T

T

A

K

D

E

A

S

P

V

L

L

P

P

G

G

S

G

-

A

-

I

-

E

-

G

-

D

-

R

Q

D

E

D

P
x
Y

T

A

E

A

T
x
R

T

L

L

R

R

R

K
x
R

I

Y

I

P

A

F

L

L

P

T

A

E

S

S

L

L

R

A

G

R

S

H

A

Y

I

A

Y

R

R

T

H

Y

G

G

D

S

R

N

T

S

P

E

A

L

W

L

L

L

G

G

D

N

S

A

R

G

Q

T

H

V

R

S

S

D

L

L

V

G

C

E

E

D

C

F

-

G

E

H

A

E

V

F

T

Y

A

E

G

A

E

E

V

L

Q

K

Y

Y

A

L

V
|
V

E
x
D

N
x
H

L

E

A
x
W

V

V

K

R

N
x
R

L

A

L
x
D

D

D

-

A

L

L

R
x
W

R

R

T

T

R

K

V

Q

G

G

M

M

binding 2-phosphoglyceric acid: R54 (= R57), Y55 (= Y58), R254 (vs. gap), I255 (vs. gap), T270 (= T266), R317 (= R313), R332 (= R329)
binding flavin-adenine dinucleotide: E33 (= E36), A34 (≠ R37), C39 (≠ T42), A40 (≠ G43), T41 (≠ A44), S42 (≠ T45), A44 (≠ R47), S45 (≠ N48), S46 (≠ H49), L48 (= L51), A172 (≠ V172), T206 (≠ A207), G207 (= G208), W209 (= W210), G231 (≠ D235), R317 (= R313), G353 (= G351), K354 (= K352), L355 (= L353), T356 (≠ M354)
binding phosphate ion: Q212 (≠ E216), G277 (≠ Y273), P279 (≠ E275), V310 (≠ L306), Y398 (≠ P390), R401 (≠ T393), R405 (≠ K397), R462 (≠ N453), D464 (≠ L455), W468 (≠ R458)
binding n-(tris(hydroxymethyl)methyl)-3-aminopropanesulfonic acid: Q157 (≠ L157), V160 (≠ R160), V455 (= V446), D456 (≠ E447), H457 (≠ N448), W459 (≠ A450)

Sites not aligning to the query:

binding flavin-adenine dinucleotide: 9, 12, 13, 14

7cyxA Crystal strcuture of glycine oxidase from bacillus cereus atcc 14579 (see paper)
30% identity, 43% coverage: 6:237/540 of query aligns to 1:230/363 of 7cyxA

query
sites
7cyxA

E

K

Y

Y

D

D

V

V

I

A

I

I

I
|
I

G
|
G

G

G

G
|
G

A
x
V

T
x
I

G

G

A

S

G

S

I

V

A

A

R

H

D

F

C

L

A

A

L

E

R

R

G

G

L

H

K

K

A

V

A

A

L

I

V
|
V

E
|
E

R
x
K

Y

Q

D

S

I

I

A

A

T

S

G
x
E

A
|
A

T
x
S

G

K

R

A

N
x
A

H

A

G
|
G

L
|
L

L

L

H

-

S

-

G

G

A

V

R

A

Y

Y

A

N

V

P

T

L

D

-

S

F

E

E

S

L

A

A

R

R

E

E

C

S

I

-

S

-

E

-

N

R

R

A

I

I

L

F

R

P

R

Q

I

L

A

A

R

A

H

V

C

L

I

R

E

E

P

K

T

T

-

G

-

V

-

D

-

I

-

G

-

Y

-

E

S

K

G

G

L

I

F

Y

-

R

I

I

T

A

L

Q

P

N

E

E

D

D

D

E

P

K

A

E

Y

R

Q

I

H

L

T

H

F

I

I

M

T

D

A

W

C

Q

Q

Q

Q

K

A

T

G

G

I

E

E

D

A

S

Q

Y

P

F

L

L

T

T

P

G

A

D

E

H

T

V

L

R

R

E

L

K

E

E

P

P

A

Y

V

L

N

S

P

E

A

S

L

I

G

G

A

A

V

V

Q

Y

V

Y

P

P

-

K

D

D

G

G

T

H

V

V

D

I

P

A

F

P

R

E

L

L

T

T

A

K

A

A

N

F

M

A

L

H

D

S

A

A

R

A

E

I

H

S

G

G

A

A

T

D

I

I

L

Y

T

E

G

Q

C

T

E

E

V
|
V

T

F

G

D

L

I

L

R

R

I

E

E

G

N

D

N

R

K

V

V

Q

T

G

G

V

V

A

I

L

T

Y

S

D

E

R

G

Q

-

R

-

E

-

P

-

L

-

S

I

L

V

R

T

A

C

Q

E

V

K

V

V

N

I

A

A

A
x
G

G
|
G

I

S

W
|
W

G

S

Q

T

R

K

I

L

A

L

E

S

Y

Y

A

F

D

H

L

R

R

D

I

W

A

G

M

T

F

Y

P

P

A

V

K

K

G

G

S

E

L

V

I

A

L

V

D

R

H

S

R

R

binding flavin-adenine dinucleotide: I7 (= I12), G8 (= G13), G10 (= G15), V11 (≠ A16), I12 (≠ T17), V30 (= V35), E31 (= E36), K32 (≠ R37), E38 (≠ G43), A39 (= A44), S40 (≠ T45), A43 (≠ N48), G45 (= G50), L46 (= L51), V171 (= V172), G200 (≠ A207), G201 (= G208), W203 (= W210)

Sites not aligning to the query:

binding flavin-adenine dinucleotide: 298, 300, 301, 326, 327, 328, 329, 330

1y56B Crystal structure of l-proline dehydrogenase from p.Horikoshii (see paper)
25% identity, 41% coverage: 22:244/540 of query aligns to 20:238/374 of 1y56B

query
sites
1y56B

A

A

R

H

D

E

C

L

A

A

L

K

R

R

G

G

L

E

K

E

A

V

A

T

L

V

V
x
I

E
|
E

R
x
K

Y

R

D

F

I

I

A

G

T

S

G

G

A
x
S

T
|
T

G
x
F

R
|
R

N

-

H

-

G

-

L

-

L
x
C

H
x
G

S
x
T

G
|
G

A

I

R

R

Y

Q

A

Q

V

F

T

N

D

D

S

E

E

A

S

N

A

V

R

R

E

V

C

M

I

K

S

R

E

S

N

V

R

E

I

L

L

W

R

K

I

Y

A

S

R

E

-

E

-

Y

-

G

H

F

C

S

I

F

E

K

P

Q

T

T

S

G

G

Y

L

L

F

F

I

L

T

L

L

Y

P

D

E

D

D

E

P

V

A

K

Y

T

Q

F

H

K

T

R

F

N

I

I

T

E

A

I

C

Q

Q

N

Q

K

A

F

G

G

I

V

E

P

A

T

Q

K

P

L

L

I

T

T

P

P

A

E

E

E

T

A

L

K

R

E

L

I

E

V

P

P

A

L

V

L

N

D

P

I

A

S

-

E

L

V

L

I

G

A

A

A

V

S

Q

W

V

N

P

P

-

T

D

D

G

G

T

K

V

A

D

D

P

P

F

F

R

E

L

A

T

T

A

T

A

A

N

F

M

A

L

V

D

K

A

A

R

K

E

E

H

Y

G

G

A

A

T

K

I

L

L

L

T

E

G

Y

C

T

E
|
E

V
|
V

T

K

G

G

L

F

L

L

R

I

E

E

G

N

D

N

R

E

V

I

Q

K

G

G

V

V

A

-

L

-

Y

-

D

-

R

-

Q

-

R

K

R

T

E

N

P

K

L

G

S

I

L

I

R

K

A

T

Q

G

V

I

V

V

N

N

A

A

A
x
T

G
x
N

I

A

W
|
W

G

A

Q

N

R

L

I

I

A

N

E

A

Y

M

A

A

D

G

L

I

-

K

-

T

R

K

I

I

A

P

M

I

F

E

P

P

A

Y

K

K

G
x
H

S

Q

L

A

L

V

I

I

L

-

D

T

H

Q

R

P

I

I

N

K

R

R

Q

G

V

T

I

I

N

N

active site: F44 (≠ G46), G47 (≠ H53), T48 (≠ S54), H224 (≠ G229)
binding flavin-adenine dinucleotide: I33 (≠ V35), E34 (= E36), K35 (≠ R37), S42 (≠ A44), T43 (= T45), R45 (= R47), C46 (≠ L52), G47 (≠ H53), G49 (= G55), E170 (= E171), V171 (= V172), T200 (≠ A207), N201 (≠ G208), W203 (= W210)
binding flavin mononucleotide: F44 (≠ G46), R45 (= R47)

Sites not aligning to the query:

active site: 239, 305, 338
binding flavin-adenine dinucleotide: 11, 13, 14, 15, 305, 306, 307, 334, 335, 336, 337, 338
binding flavin mononucleotide: 260, 301, 303

4x9mA Oxidized l-alpha-glycerophosphate oxidase from mycoplasma pneumoniae with fad bound (see paper)
22% identity, 58% coverage: 8:321/540 of query aligns to 5:328/384 of 4x9mA

query
sites
4x9mA

D

D

V

V

I

L

I

I

I

V

G
|
G

G

G

G
|
G

A

V

T
x
I

G

G

A

C

G

A

I

T

A

A

R

Y

D

E

C

L

A

S

L

Q

R

Y

G

K

L

L

K

K

A

V

A

T

L

L

V

V

E
|
E

R
x
K

Y

H

D

H

-

Y

I

L

A

A

T

Q

G
x
E

A
x
T

T
x
S

G

H

R
x
A

N
|
N

H
x
S

G

G

L
x
V

L

I

H
|
H

S

T

G

G

-

I

-

D

-

P

-

N

-

P

-

H

-

K

-

L

-

T

A

A

R

K

Y

Y

A

N

V

I

T

L

D

G

S

K

E

K

S

-

A

-

R

-

E

-

C

-

I

L

S

W

E

L

N

N

R

T

I

Y

L

F

R

K

R

R

I

L

A

G

-

F

-

P

R

R

H

Q

C

K

I

I

E

R

P

T

T

L

S

I

G

V

L

A

F

F

I

N

T

E

L

M

P

E

E

R

D

E

D

Q

P

-

A

-

Y

-

Q

-

H

-

T

-

F

-

I

L

T

E

A

V

C

L

Q

K

Q

Q

A

R

G

G

I

I

-

A

-

N

-

Q

-

I

-

N

-

L

-

E

E

D

A

I

Q

Q

P

M

L

L

T

S

P

K

A

E

E

E

T

T

L

L

R

K

L

L

E

E

P

P

A

Y

V

V

N

N

P

P

A

E

L

I

L

V

G

A

A

G

V

L

Q

K

V

I

P

E

D

G

G

S

-

W

T

A

V

I

D

D

P

P

F

V

R

L

L

A

T

S

A

K

A

C

N

L

M

A

L

L

D

A

A

A

R

Q

E

Q

H

N

G

K

A

V

T

Q

I

I

L

C

T

T

G

N

C

T

E
|
E

V
|
V

T

T

G

N

L

I

L

S

R

K

E

Q

G

-

D

-

R

-

V

V

Q

D

G

G

V

T

A

Y

L

L

Y

V

D

W

R

T

Q

N

R

N

R

E

E

T

P

T

L

P

S

S

L

F

R

K

A

V

Q

K

V

K

V

I

N

D

A

A

A
|
A

G
|
G

I

H

W
x
Y

G

A

Q

D

R

Y

I

L

A

A

E

H

Y

L

A

A

D

K

L

A

-

D

R

D

I

F

A

E

M

Q

F

T

P

T

A

R

K

R

G

G

S

Q

L
x
Y

L

V

I

V

L

V

D

T

H

N

R

Q

-

G

-

E

-

L

-

H

I

L

N

N

R

S

Q

M

V

V

I

F

N

M

R

V

C

P

R

T

K

I

P

H

A

G

D
x
K

A
x
G

D

V

I

I

L

V

V

S

P

P

G

M

-

L

D

D

T

G

I

N

S

F

L

L

I

V

G

G

T

P

T

T

S

A

M

L

H

-

I

-

P

-

Y

-

D

D

E

G

I

V

D

D

D

K

N

E

-

A

-

T

-

R

R

Y

V

I

T

T

A

K

G

D

E

A

V

P

D

C

T

M

L

L

L

T

R

K

E

I

G

G

E

K

K

H

L

M

A

V

P

P

I

S

M

L

G

N

R

I

T

N

R

N

I

A

L

L

R

I

A

S

Y

F

S

A

G

G

V
x
S

R
|
R

P

P

L

I

V

D

A

K

S

A

D

T

D

N

D

D

binding flavin-adenine dinucleotide: G10 (= G13), G12 (= G15), I14 (≠ T17), E33 (= E36), K34 (≠ R37), E41 (≠ G43), T42 (≠ A44), S43 (≠ T45), A45 (≠ R47), N46 (= N48), S47 (≠ H49), V49 (≠ L51), H51 (= H53), E176 (= E171), V177 (= V172), A209 (= A207), G210 (= G208), Y212 (≠ W210), Y234 (≠ L231), S319 (≠ V312), R320 (= R313)
binding sn-glycerol-3-phosphate: S47 (≠ H49), H51 (= H53), K258 (≠ D251), G259 (≠ A252), R320 (= R313)

Sites not aligning to the query:

P75063 Glycerol 3-phosphate oxidase; GlpO; L-alpha-glycerophosphate oxidase; EC 1.1.3.21 from Mycoplasma pneumoniae (strain ATCC 29342 / M129 / Subtype 1) (Mycoplasmoides pneumoniae) (see paper)
22% identity, 58% coverage: 8:321/540 of query aligns to 5:328/384 of P75063

query
sites
P75063

D

D

V

V

I

L

I

I

I

V

G

G

A

V

T
x
I

G

G

A

C

G

A

I

T

A

A

R

Y

D

E

C

L

A

S

L

Q

R

Y

G

K

L

L

K

K

A

V

A

T

L

L

V

V

E
|
E

R

K

Y

H

D

H

-

Y

I

L

A

A

T

Q

G

E

A
x
T

T
x
S

G

H

R

A

N

N

H
x
S

G
|
G

L
x
V

L

I

H

H

S

T

G

G

-

I

-

D

-

P

-

N

-

P

-

H

-

K

-

L

-

T

A

A

R

K

Y

Y

A

N

V

I

T

L

D

G

S

K

E

K

S

-

A

-

R

-

E

-

C

-

I

L

S

W

E

L

N

N

R

T

I

Y

L

F

R

K

R

R

I

L

A

G

-

F

-

P

R

R

H

Q

C

K

I

I

E

R

P

T

T

L

S

I

G

V

L

A

F

F

I

N

T

E

L

M

P

E

E

R

D

E

D

Q

P

-

A

-

Y

-

Q

-

H

-

T

-

F

-

I

L

T

E

A

V

C

L

Q

K

Q

Q

A

R

G

G

I

I

-

A

-

N

-

Q

-

I

-

N

-

L

-

E

E

D

A

I

Q

Q

P

M

L

L

T

S

P

K

A

E

E

E

T

T

L

L

R

K

L

L

E

E

P

P

A

Y

V

V

N

N

P

P

A

E

L

I

L

V

G

A

A

G

V

L

Q

K

V

I

P

E

D

G

G

S

-

W

T

A

V

I

D

D

P

P

F

V

R

L

L

A

T

S

A

K

A

C

N

L

M

A

L

L

D

A

A

A

R

Q

E

Q

H

N

G

K

A

V

T

Q

I

I

L

C

T

T

G

N

C

T

E

E

V
|
V

T

T

G

N

L

I

L

S

R

K

E

Q

G

-

D

-

R

-

V

V

Q

D

G

G

V

T

A

Y

L

L

Y

V

D

W

R

T

Q

N

R

N

R

E

E

T

P

T

L

P

S

S

L

F

R

K

A

V

Q

K

V

K

V

I

N

D

A

A

G

G

I

H

W

Y

G

A

Q

D

R

Y

I

L

A

A

E

H

Y

L

A

A

D

K

L

A

-

D

R

D

I

F

A

E

M

Q

F

T

P

T

A

R

K

R

G

G

S

Q

L

Y

L

V

I

V

L

V

D

T

H

N

R

Q

-

G

-

E

-

L

-

H

I

L

N

N

R

S

Q

M

V

V

I

F

N

M

R

V

C

P

R

T

K

I

P

H

A

G

D

K

A

G

D

V

I

I

L

V

V

S

P

P

G

M

-

L

D

D

T

G

I

N

S

F

L

L

I

V

G

G

T

P

T

T

S

A

M

L

H

-

I

-

P

-

Y

-

D

D

E

G

I

V

D

D

D

K

N

E

-

A

-

T

-

R

R

Y

V

I

T

T

A

K

G

D

E

A

V

P

D

C

T

M

L

L

L

T

R

K

E

I

G

G

E

K

K

H

L

M

A

V

P

P

I

S

M

L

G

N

R

I

T

N

R

N

I

A

L

L

R

I

A

S

Y

F

S

A

G

G

V

S

R

R

P

P

L

I

V

D

A

K

S

A

D

T

D

N

D

D

I14 (≠ T17) binding
E33 (= E36) binding
TS 42:43 (≠ AT 44:45) binding
SGV 47:49 (≠ HGL 49:51) binding
V177 (= V172) binding

Sites not aligning to the query:

346:347 binding
352 binding

4pabB Crystal structure of the precursor form of rat dmgdh complexed with tetrahydrofolate (see paper)
26% identity, 42% coverage: 8:233/540 of query aligns to 7:230/824 of 4pabB

query
sites
4pabB

D

E

V

T

I

V

I

I

I
|
I

G
|
G

G

G

G
|
G

A
x
C

T
x
V

G

G

A

V

G

S

I

L

A

A

R

Y

D

H

C

L

A

A

L

K

R

A

G

G

L

M

K

R

-

D

A

V

L

L

V
x
L

E
|
E

R
x
K

Y

S

D

E

I

L

A

T

T

A

G
|
G

A
x
S

T
|
T

G

W

R
x
H

N
x
A

H
x
A

G
|
G

L
|
L

-

T

-
x
T

-

Y

L

F

H

H

S

P

G

G

A

I

R

N

Y

L

A

K

V

K

T

I

D

H

S

Y

E

D

S

S

A

I

R

K

E

L

C

Y

I

E

S

R

-

L

E

E

N

E

R

E

I

T

L

G

R

Q

R

V

I

V

A

G

R

F

H

H

C

-

I

-

E

Q

P

P

T

G

S

S

G

I

L

R

F

L

I

A

T

T

L

T

P

P

E

E

-

R

-

V

D

D

D
x
E

P

F

A

K

Y

Y

Q

Q

H

-

T

-

F

-

I

M

T

T

A

R

C

T

Q

N

Q

W

A

H

G

A

I

T

E

E

A

Q

Q

Y

P

I

L

I

T

E

P

P

A

E

E

K

T

I

L

H

R

E

L

L

E

F

P

P

A

L

V

L

N

N

-

M

-

D

P

K

A

I

L

L

L

A

G

G

A

L

V

Y

Q

N

V

P

P

G

D

D

G

G

T

H

V

I

D

D

P

P

F

Y

R

S

L

L

T

T

A

M

A

A

N

L

M

A

L

T

D

G

A

A

R

R

E

K

H

Y

G

G

A

V

T

L

I

L

L

K

T

Y

G

P

C

A

E

P

V
|
V

T

T

G

S

L

L

L

K

R

P

E

R

G

P

D

D

R

-

V

-

Q

-

G

-

V

-

A

G

L

T

Y

W

D

D

R

V

Q

E

R

T

R

P

E

Q

P

G

L

-

S

S

L

V

R

R

A

A

Q

N

V

R

V

I

V

V

N

N

A

A

A
|
A

G
|
G

I

F

W
|
W

G

A

Q

R

R

E

I

V

A

G

E

K

Y

M

A

I

D

G

L

L

R

D

I

H

A

P

M

L

F

I

P

P

A

V

K

Q

G
x
H

S

Q

L
x
Y

L

V

I

V

active site: T53 (vs. gap), E102 (≠ D98), H226 (≠ G229)
binding flavin-adenine dinucleotide: I11 (= I12), G12 (= G13), G14 (= G15), C15 (≠ A16), V16 (≠ T17), L35 (≠ V35), E36 (= E36), K37 (≠ R37), G43 (= G43), S44 (≠ A44), T45 (= T45), H47 (≠ R47), A48 (≠ N48), A49 (≠ H49), G50 (= G50), L51 (= L51), V175 (= V172), A204 (= A207), G205 (= G208), W207 (= W210), H226 (≠ G229), Y228 (≠ L231)

Sites not aligning to the query:

active site: 255, 536
binding flavin-adenine dinucleotide: 326, 328, 353, 355, 356, 357, 358
binding (6s)-5,6,7,8-tetrahydrofolate: 523, 536, 538, 550, 612, 613, 632, 639, 680, 700

Q63342 Dimethylglycine dehydrogenase, mitochondrial; ME2GLYDH; EC 1.5.8.4 from Rattus norvegicus (Rat) (see 2 papers)
26% identity, 42% coverage: 8:233/540 of query aligns to 44:267/857 of Q63342

query
sites
Q63342

D

E

V

T

I

V

I

I

G

G

A
x
C

T
x
V

G

G

A

V

G

S

I

L

A

A

R

Y

D

H

C

L

A

A

L

K

R

A

G

G

L

M

K

R

-

D

A

V

L

L

V

L

E
|
E

R
x
K

Y

S

D

E

I

L

A

T

T

A

G
|
G

A
x
S

T
|
T

G
x
W

R
x
H

N
x
A

H
x
A

G
|
G

L
|
L

-

T

-

Y

L

F

H

H

S

P

G

G

A

I

R

N

Y

L

A

K

V

K

T

I

D

H

S

Y

E

D

S

S

A

I

R

K

E

L

C

Y

I

E

S

R

-

L

E

E

N

E

R

E

I

T

L

G

R

Q

R

V

I

V

A

G

R

F

H

H

C

-

I

-

E

Q

P

P

T

G

S

S

G

I

L

R

F

L

I

A

T

T

L

T

P

P

E

E

-

R

-

V

D

D

D

E

P

F

A

K

Y

Y

Q

Q

H

-

T

-

F

-

I

M

T

T

A

R

C

T

Q

N

Q

W

A

H

G

A

I

T

E

E

A

Q

Q

Y

P

I

L

I

T

E

P

P

A

E

E

K

T

I

L

H

R

E

L

L

E

F

P

P

A

L

V

L

N

N

-

M

-

D

P

K

A

I

L

L

L

A

G

G

A

L

V

Y

Q

N

V

P

P

G

D

D

G

G

T

H

V

I

D

D

P

P

F

Y

R

S

L

L

T

T

A

M

A

A

N

L

M

A

L

T

D

G

A

A

R

R

E

K

H

Y

G

G

A

V

T

L

I

L

L

K

T

Y

G

P

C

A

E

P

V
|
V

T

T

G

S

L

L

L

K

R

P

E

R

G

P

D

D

R

-

V

-

Q

-

G

-

V

-

A

G

L

T

Y

W

D

D

R

V

Q

E

R

T

R

P

E

Q

P

G

L

-

S

S

L

V

R

R

A

A

Q

N

V

R

V

I

V

V

N

N

A

A

G

G

I

F

W
|
W

G

A

Q

R

R

E

I

V

A

G

E

K

Y

M

A

I

D

G

L

L

R

D

I

H

A

P

M

L

F

I

P

P

A

V

K

Q

G

H

S

Q

L

Y

L

V

I

V

CV 52:53 (≠ AT 16:17) binding
EK 73:74 (≠ ER 36:37) binding
80:88 (vs. 43:51, 44% identical) binding
H84 (≠ R47) modified: Tele-8alpha-FAD histidine
V212 (= V172) binding
W244 (= W210) binding

Sites not aligning to the query:

390:395 binding
573:575 binding
669 binding
676:678 binding
737 binding

Q9UI17 Dimethylglycine dehydrogenase, mitochondrial; ME2GLYDH; EC 1.5.8.4 from Homo sapiens (Human) (see 4 papers)
26% identity, 42% coverage: 8:233/540 of query aligns to 51:274/866 of Q9UI17

query
sites
Q9UI17

D

E

V

T

I

V

I

I

G

G

A
x
C

T
x
V

G

G

A

V

G

S

I

L

A

A

R

Y

D

H

C

L

A

A

L

K

R

A

G

G

L

M

K

K

-

D

A

V

L

L

V

L

E
|
E

R
x
K

Y

S

D

E

I

L

A

T

T

A

G
|
G

A
x
S

T
|
T

G
x
W

R
x
H

N
x
A

H
x
A

G
|
G

L
|
L

-

T

-

Y

L

F

H

H

S

P

G

G

A

I

R

N

Y

L

A

K

V

K

T

I

D
x
H

S

Y

E

D

S

S

A

I

R

K

E

L

C

Y

I

E

S

K

-

L

E

E

N

E

R

E

I

T

L

G

R

Q

R

V

I

V

A

G

R

F

H

H

C

-

I

-

E

Q

P

P

T

G

S

S

G

I

L

R

F

L

I

A

T

T

L

T

P

P

E

V

-

R

-

V

D

D

D

E

P

F

A

K

Y

Y

Q

Q

H

-

T

-

F

-

I

M

T

T

A

R

C

T

Q

G

Q

W

A

H

G

A

I

T

E

E

A

Q

Q

Y

P

L

L

I

T

E

P

P

A

E

E

K

T

I

L

Q

R

E

L

M

E

F

P

P

-

L

A

N

V

M

N

N

P

K

A

V

L

L

L

A

G

G

A

L

V

Y

Q

N

V

P

P

G

D

D

G

G

T

H

V

I

D

D

P

P

F

Y

R

S

L

L

T

T

A

M

A

A

N

L

M

A

L

A

D

G

A

A

R

R

E

K

H

C

G

G

A

A

T

L

I

L

L

K

T

Y

G

P

C

A

E

P

V
|
V

T

T

G

S

L

L

L

K

R

A

E

R

G

S

D

D

R

-

V

-

Q

-

G

-

V

-

A

G

L

T

Y

W

D

D

R

V

Q

E

R

T

R

P

E

Q

P

G

L

-

S

S

L

M

R

R

A

A

Q

N

V

R

V

I

V

V

N

N

A

A

G

G

I

F

W

W

G

A

Q

R

R

E

I

V

A

G

E

K

Y

M

A

I

D

G

L

L

R

E

I

H

A

P

M

L

F

I

P

P

A

V

K

Q

G

H

S

Q

L

Y

L

V

I

V

CV 59:60 (≠ AT 16:17) binding
EK 80:81 (≠ ER 36:37) binding
87:95 (vs. 43:51, 44% identical) binding
H91 (≠ R47) modified: Tele-8alpha-FAD histidine
H109 (≠ D62) to R: in DMGDHD; shows 10 fold lower catalytic efficiency due to lower cofactor saturation and reduced thermal stability; dbSNP:rs121908331
V219 (= V172) binding

Sites not aligning to the query:

279 S → P: in dbSNP:rs532964
397:402 binding
530 A → G: in dbSNP:rs1805073
646 S → P: in dbSNP:rs1805074

3dmeA Crystal structure of conserved exported protein from bordetella pertussis. Northeast structural genomics target ber141
31% identity, 39% coverage: 6:215/540 of query aligns to 2:211/366 of 3dmeA

query
sites
3dmeA

E

D

Y

I

D

D

V

C

I

I

I

V

I
|
I

G
|
G

G

A

G
|
G

A
x
V

T
x
V

G

G

A

L

G

A

I

I

A

A

R

R

D

A

C

L

A

A

L

A

R

G

G

G

L

H

K

E

A

V

A

L

L

V

V

A

E
|
E

R
x
A

Y

A

D

E

-

G

I

I

A

G

T

T

G

G

A
x
T

T
x
S

G

S

R
|
R

N
|
N

H
x
S

G

E

L
x
V

L

I

H
|
H

S

A

G

G

A

I

R

Y

Y

Y

A

P

V

-

T

A

D

D

S

S

E

L

S

K

A

A

R

R

E

L

C

C

I

V

S

R

E

G

N

K

R

H

I

L

L

L

R

Y

R

E

I

Y

-

C

-

A

A

A

R

R

H

G

C

V

I

P

E

H

P

Q

T

R

S

L

G

G

L

K

F

L

I

I

T

V

L

A

P

T

E

S

D

D

D

A

P

E

A

A

Y

S

Q

Q

H

L

T

D

F

S

I

I

T

-

A

-

C

A

Q

R

A

A

G

G

I

A

E

N

A

G

-

V

-

D

-

L

Q

Q

P

H

L

I

T

D

P

G

A

A

E

A

T

A

L

R

R

R

L

L

E

E

P

P

A

A

V

L

N

H

P

-

A

C

L

T

L

A

G

A

A

L

V

V

Q

S

V

P

P

S

D

T

G

G

T

I

V

V

D

D

P

S

F

H

R

A

L

L

T

M

A

L

A

A

N

Y

M

Q

L

G

D

D

A

A

R

E

E

S

H

D

G

G

A

A

T

Q

I

L

L

V

T

F

G

H

C

-

E

-

V

-

T

T

G
x
P

L
|
L

L

I

R

A

E

G

G

R

D

V

R

R

V

P

Q

E

G

G

V

G

A

F

L

E

Y

L

D

D

R

F

Q

G

R

G

R

A

E

E

P

P

L

M

S

T

L

L

R

S

A

C

Q

R

V

V

V

L

V

I

N

N

A

A

A
|
A

G

G

I

L

W

H

G

A

Q

P

R

G

I

L

A

A

binding flavin-adenine dinucleotide: I8 (= I12), G9 (= G13), G11 (= G15), V12 (≠ A16), V13 (≠ T17), E32 (= E36), A33 (≠ R37), T41 (≠ A44), S42 (≠ T45), R44 (= R47), N45 (= N48), S46 (≠ H49), V48 (≠ L51), H50 (= H53), P170 (≠ G174), L171 (= L175), A203 (= A207)

Sites not aligning to the query:

binding flavin-adenine dinucleotide: 313, 314, 346, 347, 348, 349, 350, 351, 352

1pj7A Structure of dimethylglycine oxidase of arthrobacter globiformis in complex with folinic acid (see paper)
26% identity, 40% coverage: 9:226/540 of query aligns to 4:219/827 of 1pj7A

query
sites
1pj7A

V

I

I

V

I

I

G
|
G

G

A

G
|
G

A
x
I

T
x
V

G

G

A

T

G

N

I

L

A

A

R

D

D

E

C

L

A

V

L

T

R

R

G

G

L

W

K

N

A

N

A

I

L

T

V

V

-

L

-
x
D

-
x
Q

E

G

R

P

Y

L

D

N

I

M

A

P

T

G

G
|
G

A
x
S

T
|
T

G

S

R
x
H

N

A

H
x
P

G

G

L
|
L

L

V

H

F

S

Q

G

-

A

T

R

N

Y

P

A

S

V

K

T

T

D

M

S

A

E

S

S

F

A

A

R

K

E

Y

C

T

I

V

S

E

E

K

N

L

R

L

I

S

L

L

R

T

R

E

I

D

A

G

R

V

H

S

C

C

I

F

E

N

P

Q

T

V

S

G

G

G

L

L

F

E

I

V

T

A

L

T

P

T

E

E

D

T

D

R

P

L

A

A

Y

D

Q

L

H

K

T

R

F

K

I

L

T

G

A

Y

C

A

Q

A

A

W

G

G

I

I

E

E

A

G

Q

R

P

L

L

L

T

S

P

P

A

A

E

E

T

C

L

Q

R

E

L

L

E

Y

P

P

A

L

V

L

N

D

-

G

P

E

A

N

L

I

L

L

G

G

A

G

V

L

Q

H

V

V

P

P

-

S

D

D

G

G

T

L

V

A

D

S

P

A

F

A

R

R

L

A

T

V

A

Q

A

L

N

L

M

I

L

K

D

R

A

T

R

E

E

S

H

A

G

G

A

V

T

T

I

Y

L

R

T

G

G

S

C

T

E
x
T

V
|
V

T

T

G

G

L

I

L

E

R

Q

E

S

G

G

D

G

R

R

V

V

Q

T

G

G

V

V

A

Q

L

T

Y

A

D

D

R

G

Q

-

R

-

E

-

P

-

L

-

S

V

L

I

R

P

A

A

Q

D

V

I

V

V

N

S

A

C

A
|
A

G
|
G

I

F

W
|
W

G

G

Q

A

R

K

I

I

A

G

E

A

Y

M

A

I

D

G

L

M

R

A

I

V

A

P

M

L

F

L

P

P

binding flavin-adenine dinucleotide: G8 (= G13), G10 (= G15), I11 (≠ A16), V12 (≠ T17), D32 (vs. gap), Q33 (vs. gap), G41 (= G43), S42 (≠ A44), T43 (= T45), H45 (≠ R47), P47 (≠ H49), L49 (= L51), T170 (≠ E171), V171 (= V172), A200 (= A207), G201 (= G208), W203 (= W210)

Sites not aligning to the query:

active site: 222, 256, 549
binding flavin-adenine dinucleotide: 222, 256, 331, 357, 358, 359, 360
binding N-[4-({[(6S)-2-amino-5-formyl-4-oxo-3,4,5,6,7,8-hexahydropteridin-6-yl]methyl}amino)benzoyl]-L-glutamic acid: 505, 536, 549, 551, 563, 629, 648, 655, 696

1pj6A Crystal structure of dimethylglycine oxidase of arthrobacter globiformis in complex with folic acid (see paper)
26% identity, 40% coverage: 9:226/540 of query aligns to 5:220/828 of 1pj6A

query
sites
1pj6A

V

I

I

V

I

I

G
|
G

G

A

G
|
G

A
x
I

T
x
V

G

G

A

T

G

N

I

L

A

A

R

D

D

E

C

L

A

V

L

T

R

R

G

G

L

W

K

N

A

N

A

I

L

T

V

V

-

L

-
x
D

-
x
Q

E

G

R

P

Y

L

D

N

I

M

A

P

T

G

G
|
G

A
x
S

T
|
T

G

S

R
x
H

N

A

H
x
P

G

G

L
|
L

L

V

H

F

S

Q

G

-

A

T

R

N

Y

P

A

S

V

K

T

T

D

M

S

A

E

S

S

F

A

A

R

K

E

Y

C

T

I

V

S

E

E

K

N

L

R

L

I

S

L

L

R

T

R

E

I

D

A

G

R

V

H

S

C

C

I

F

E

N

P

Q

T

V

S

G

G

G

L

L

F

E

I

V

T

A

L

T

P

T

E

E

D

T

D

R

P

L

A

A

Y

D

Q

L

H

K

T

R

F

K

I

L

T

G

A

Y

C

A

Q

A

A

W

G

G

I

I

E

E

A

G

Q

R

P

L

L

L

T

S

P

P

A

A

E

E

T

C

L

Q

R

E

L

L

E

Y

P

P

A

L

V

L

N

D

-

G

P

E

A

N

L

I

L

L

G

G

A

G

V

L

Q

H

V

V

P

P

-

S

D

D

G

G

T

L

V

A

D

S

P

A

F

A

R

R

L

A

T

V

A

Q

A

L

N

L

M

I

L

K

D

R

A

T

R

E

E

S

H

A

G

G

A

V

T

T

I

Y

L

R

T

G

G

S

C

T

E

T

V
|
V

T

T

G

G

L

I

L

E

R

Q

E

S

G

G

D

G

R

R

V

V

Q

T

G

G

V

V

A

Q

L

T

Y

A

D

D

R

G

Q

-

R

-

E

-

P

-

L

-

S

V

L

I

R

P

A

A

Q

D

V

I

V

V

N

S

A

C

A
|
A

G
|
G

I

F

W
|
W

G

G

Q

A

R

K

I

I

A

G

E

A

Y

M

A

I

D

G

L

M

R

A

I

V

A

P

M

L

F

L

P

P

binding flavin-adenine dinucleotide: G9 (= G13), G11 (= G15), I12 (≠ A16), V13 (≠ T17), D33 (vs. gap), Q34 (vs. gap), G42 (= G43), S43 (≠ A44), T44 (= T45), H46 (≠ R47), P48 (≠ H49), L50 (= L51), V172 (= V172), A201 (= A207), G202 (= G208), W204 (= W210)

Sites not aligning to the query:

active site: 223, 257, 550
binding flavin-adenine dinucleotide: 223, 257, 331, 332, 358, 359, 360, 361

Q9AGP8 Dimethylglycine oxidase; DMGO; EC 1.5.3.10 from Arthrobacter globiformis (see 2 papers)
26% identity, 40% coverage: 9:226/540 of query aligns to 7:222/830 of Q9AGP8

query
sites
Q9AGP8

V

I

I

V

I

I

G

G

G

A

G

G

A
x
I

T
x
V

G

G

A

T

G

N

I

L

A

A

R

D

D

E

C

L

A

V

L

T

R

R

G

G

L

W

K

N

A

N

A

I

L

T

V

V

-

L

-
x
D

-
x
Q

E

G

R

P

Y

L

D

N

I

M

A

P

T

G

G

G

A
x
S

T
|
T

G
x
S

R
x
H

N

A

H

P

G

G

L
|
L

L

V

H

F

S

Q

G

-

A

T

R

N

Y

P

A

S

V

K

T

T

D

M

S

A

E

S

S

F

A

A

R

K

E

Y

C

T

I

V

S

E

E

K

N

L

R

L

I

S

L

L

R

T

R

E

I

D

A

G

R

V

H

S

C

C

I

F

E

N

P

Q

T

V

S

G

G

G

L

L

F

E

I

V

T

A

L

T

P

T

E

E

D

T

D

R

P

L

A

A

Y

D

Q

L

H

K

T

R

F

K

I

L

T

G

A

Y

C

A

Q

A

A

W

G

G

I

I

E

E

A

G

Q

R

P

L

L

L

T

S

P

P

A

A

E

E

T

C

L

Q

R

E

L

L

E

Y

P

P

A

L

V

L

N

D

-

G

P

E

A

N

L

I

L

L

G

G

A

G

V

L

Q

H

V

V

P

P

-

S

D

D

G

G

T

L

V

A

D

S

P

A

F

A

R

R

L

A

T

V

A

Q

A

L

N

L

M

I

L

K

D

R

A

T

R

E

E

S

H

A

G

G

A

V

T

T

I

Y

L

R

T

G

G

S

C

T

E

T

V
|
V

T

T

G

G

L

I

L

E

R

Q

E

S

G

G

D

G

R

R

V

V

Q

T

G

G

V

V

A

Q

L

T

Y

A

D

D

R

G

Q

-

R

-

E

-

P

-

L

-

S

V

L

I

R

P

A

A

Q

D

V

I

V

V

N

S

A

C

A

A

G

G

I

F

W

W

G

G

Q

A

R

K

I

I

A

G

E

A

Y

M

A

I

D

G

L

M

R

A

I

V

A

P

M

L

F

L

P

P

IV 14:15 (≠ AT 16:17) binding
DQ 35:36 (vs. gap) binding
STSH 45:48 (≠ ATGR 44:47) binding
L52 (= L51) binding
V174 (= V172) binding

Sites not aligning to the query:

225 Important for catalytic activity; H→Q: Reduces catalytic efficiency 3-fold and substrate affinity 30-fold.
259 Important for catalytic activity; binding ; Y→F: Reduces catalytic efficiency 225-fold and substrate affinity 25-fold.
360:363 binding
539 binding
552 Important for catalytic activity; D→A: No effect on the activity.; D→N: Reduces activity 3-fold.

3gsiA Crystal structure of d552a dimethylglycine oxidase mutant of arthrobacter globiformis in complex with tetrahydrofolate (see paper)
26% identity, 40% coverage: 9:226/540 of query aligns to 4:219/827 of 3gsiA

query
sites
3gsiA

V

I

I

V

I

I

G

G

G

A

G
|
G

A
x
I

T
x
V

G

G

A

T

G

N

I

L

A

A

R

D

D

E

C

L

A

V

L

T

R

R

G

G

L

W

K

N

A

N

A

I

L

T

V

V

-

L

-
x
D

-
x
Q

E

G

R

P

Y

L

D

N

I

M

A

P

T

G

G
|
G

A
x
S

T
|
T

G

S

R
x
H

N

A

H
x
P

G

G

L
|
L

L

V

H

F

S

Q

G

-

A

T

R

N

Y

P

A

S

V

K

T

T

D

M

S

A

E

S

S

F

A

A

R

K

E

Y

C

T

I

V

S

E

E

K

N

L

R

L

I

S

L

L

R

T

R

E

I

D

A

G

R

V

H

S

C

C

I

F

E

N

P

Q

T

V

S

G

G

G

L

L

F

E

I

V

T

A

L

T

P

T

E

E

D

T

D

R

P

L

A

A

Y

D

Q

L

H

K

T

R

F

K

I

L

T

G

A

Y

C

A

Q

A

A

W

G

G

I

I

E

E

A

G

Q

R

P

L

L

L

T

S

P

P

A

A

E

E

T

C

L

Q

R

E

L

L

E

Y

P

P

A

L

V

L

N

D

-

G

P

E

A

N

L

I

L

L

G

G

A

G

V

L

Q

H

V

V

P

P

-

S

D

D

G

G

T

L

V

A

D

S

P

A

F

A

R

R

L

A

T

V

A

Q

A

L

N

L

M

I

L

K

D

R

A

T

R

E

E

S

H

A

G

G

A

V

T

T

I

Y

L

R

T

G

G

S

C

T

E
x
T

V
|
V

T

T

G

G

L

I

L

E

R

Q

E

S

G

G

D

G

R

R

V

V

Q

T

G

G

V

V

A

Q

L

T

Y

A

D

D

R

G

Q

-

R

-

E

-

P

-

L

-

S

V

L

I

R

P

A

A

Q

D

V

I

V

V

N

S

A

C

A
|
A

G
|
G

I

F

W
|
W

G

G

Q

A

R

K

I

I

A

G

E

A

Y

M

A

I

D

G

L

M

R

A

I

V

A

P

M

L

F

L

P

P

binding flavin-adenine dinucleotide: G10 (= G15), I11 (≠ A16), V12 (≠ T17), D32 (vs. gap), Q33 (vs. gap), G41 (= G43), S42 (≠ A44), T43 (= T45), H45 (≠ R47), P47 (≠ H49), L49 (= L51), T170 (≠ E171), V171 (= V172), A200 (= A207), G201 (= G208), W203 (= W210)

Sites not aligning to the query:

active site: 222, 256, 549
binding flavin-adenine dinucleotide: 222, 256, 330, 331, 332, 357, 358, 359, 360
binding magnesium ion: 254, 409
binding (6s)-5,6,7,8-tetrahydrofolate: 505, 536, 551, 563, 629, 648, 655, 696

4yshB Crystal structure of glycine oxidase from geobacillus kaustophilus
27% identity, 58% coverage: 4:314/540 of query aligns to 1:308/368 of 4yshB

query
sites
4yshB

S

T

R

H

E

R

Y

Y

D

D

V

V

I

A

I

I

I
x
V

G
|
G

G

G

G
|
G

A

V

T
x
I

G

G

A

A

G

A

I

I

A

G

R

F

D

E

C

L

A

A

L

K

R

R

G

R

L

H

K

R

A

V

A

A

L

I

V
x
F

E
|
E

R
x
K

Y

G

D

T

I

M

A

G

T

S

G
|
G

A
|
A

T
x
S

G

S

R

A

N
x
A

H
x
A

G

G

L
x
M

L
|
L

H

G

S

A

G
x
Q

A

S

R

E

Y

F

A

S

V

-

T

T

D

S

S

S

E

P

S

L

A

V

R

P

E

L

C

A

I

L

S

Q

E

S

N

R

R

A

I

L

L

M

R

P

R

A

I

L

A

A

R

E

H

E

C

L

I

R

E

E

P

R

T

T

S

G

-

I

-

D

-

I

-

G

-

L

-

V

-

E

-

K

-

G

-

L

-

I

G

K

L

L

F

A

I

T

T

T

L

E

P

E

E

E

D

A

D

D

P

D

A

L

Y

Y

Q

R

H

H

T

-

F

-

I

Y

T

T

A

F

C

W

Q

R

Q

G

A

I

G

G

I

E

E

P

A

V

Q

Q

P

W

L

L

T

T

P

K

A

G

E

E

T

A

L

L

R

E

L

M

E

E

P

P

A

R

V

L

N

A

P

E

A

A

L

L

L

A

G

G

A

A

V

M

Q

Y

V

I

P

P

-

G

D

D

G

G

T

Q

V

V

D

S

P

A

F

P

R

D

L

L

T

A

A

A

N

L

M

A

L

Y

D

A

A

A

R

A

E

S

H

A

G

G

A

A

T

C

I

L

L

Y

T

E

G

Y

C

T

E

E

V
|
V

T

F

G

D

L

I

L

R

R

S

E

D

G

S

-

S

-

G

-

H

-

V

-

L

D

D

R

T

V

T

Q

G

G

G

V

-

A

-

L

-

Y

-

D

-

R

-

Q

-

R

-

E

-

P

-

L

-

S

T

L

F

R

A

A

A

Q

E

V

A

V

V

N

I

A

A

A
x
S

G

G

I

A

W
|
W

G

A

Q

A

R

R

I

L

A

G

E

A

Y

R

A

V

D

G

L

L

R

S

I

L

A

S

M

V

F

Y

P

P

A

V

K

K

G

G

S

E

L

C

L

V

I

M

L

V

D

-

H

-

R

R

I

A

N

P

R

V

Q

P

V

L

I

L

N

Q

R

T

C

T

R

V

K

F

P

A

A

K

D

N

A
x
G

D

C

I
x
Y

L

I

V

V

P

P

-

K

-

S

G

G

D

N

T

R

I

L

S

-

L

L

I
|
I

G

G

T
x
A

T

T

S

S

M

T

H

P

I

G

P

T

Y

F

D

-

E

-

I

-

D

-

D

D

N

R

R

R

V

V

T

S

A

A

G

G

E

G

V

V

D

M

T

N

L
|
L

L

L

R

H

E

R

G

A

E

A

K

H

L

L

A

V

P

P

I

D

M

I

G

E

R

Q

T

A

R

E

I

W

L

V

R

A

A

S

Y

W

S

S

G
|
G

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I

R
|
R

P

P

active site: A45 (≠ N48), M48 (≠ L51), L49 (= L52), Q52 (≠ G55), I262 (= I264), L283 (= L289), G305 (= G311)
binding flavin-adenine dinucleotide: V9 (≠ I12), G10 (= G13), G12 (= G15), I14 (≠ T17), F32 (≠ V35), E33 (= E36), K34 (≠ R37), G40 (= G43), A41 (= A44), S42 (≠ T45), A45 (≠ N48), A46 (≠ H49), M48 (≠ L51), V178 (= V172), S206 (≠ A207), W209 (= W210), R307 (= R313)
binding glycine: G249 (≠ A252), Y251 (≠ I254), Y251 (≠ I254), A264 (≠ T266), R307 (= R313)

Sites not aligning to the query:

active site: 335, 338
binding flavin-adenine dinucleotide: 332, 334, 335, 336, 337, 338
binding glycine: 334, 334

Query Sequence

>BWI76_RS19965 BWI76_RS19965 sn-glycerol-3-phosphate dehydrogenase subunit A
MADSREYDVIIIGGGATGAGIARDCALRGLKAALVERYDIATGATGRNHGLLHSGARYAV
TDSESARECISENRILRRIARHCIEPTSGLFITLPEDDPAYQHTFITACQQAGIEAQPLT
PAETLRLEPAVNPALLGAVQVPDGTVDPFRLTAANMLDAREHGATILTGCEVTGLLREGD
RVQGVALYDRQRREPLSLRAQVVVNAAGIWGQRIAEYADLRIAMFPAKGSLLILDHRINR
QVINRCRKPADADILVPGDTISLIGTTSMHIPYDEIDDNRVTAGEVDTLLREGEKLAPIM
GRTRILRAYSGVRPLVASDDDPSGRSVSRGIVLLDHAQRDGMEGFITITGGKLMTYRLMA
EWATDAVCRKLGNTAPCTTADAPLPGSQEPTETTLRKIIALPASLRGSAIYRHGDRTPAW
LGDSRQHRSLVCECEAVTAGEVQYAVENLAVKNLLDLRRRTRVGMGTCQGELCACRAAGL
LARFNVTTSARSLTQLSEFLNERWKGVQPVAWGDALRESEFTRWVYLGLCGLQKEHQDEV

Or try a new SitesBLAST search

SitesBLAST's Database

SitesBLAST's database includes (1) SwissProt entries with experimentally-supported functional features; and (2) protein structures with bound ligands, from the BioLip database.

by Morgan Price, Arkin group
Lawrence Berkeley National Laboratory