SitesBLAST
Comparing BWI76_RS20335 FitnessBrowser__Koxy:BWI76_RS20335 to proteins with known functional sites using BLASTp with E ≤ 0.001.
Or try Sites on a Tree, PaperBLAST, Conserved Domains, or compare to all protein structures
Found 20 (the maximum) hits to proteins with known functional sites (download)
1hslA Refined 1.89 angstroms structure of the histidine-binding protein complexed with histidine and its relationship with many other active transport(slash)chemosensory receptors (see paper)
93% identity, 91% coverage: 23:259/260 of query aligns to 1:237/238 of 1hslA
P02910 Histidine-binding periplasmic protein; HBP from Salmonella typhimurium (strain LT2 / SGSC1412 / ATCC 700720) (see 2 papers)
93% identity, 91% coverage: 24:259/260 of query aligns to 24:259/260 of P02910
- E40 (= E40) mutation to K: Decrease in ATPase-inducing activity and histidine transport; when associated with K-47.
- K42 (= K42) mutation to E: Increases ATPase-inducing activity and histidine transport; when associated with K-47.
- E47 (= E47) mutation to K: Decrease in ATPase-inducing activity and histidine transport; when associated with K-40. Increases ATPase-inducing activity and histidine transport; when associated with E-42.
- D171 (= D171) mutation D->A,N: Strong decrease in ATPase-inducing activity and histidine transport.
- R176 (= R176) mutation R->D,S: Strong decrease in ATPase-inducing activity and histidine transport.
- D178 (= D178) mutation to A: Slight decrease in ATPase-inducing activity and histidine transport.
Sites not aligning to the query:
P0AEU0 Histidine-binding periplasmic protein; HBP from Escherichia coli (strain K12) (see 3 papers)
92% identity, 91% coverage: 24:259/260 of query aligns to 24:259/260 of P0AEU0
- C60 (= C60) modified: Disulfide link with 67
- C67 (= C67) modified: Disulfide link with 60
- S91 (= S91) binding L-histidine
- S92 (= S92) binding L-histidine
- S94 (= S94) binding L-histidine
- R99 (= R99) binding L-histidine
- T143 (= T143) binding L-histidine
- D183 (= D183) binding L-histidine
Sites not aligning to the query:
P02911 Lysine/arginine/ornithine-binding periplasmic protein; LAO-binding protein from Salmonella typhimurium (strain LT2 / SGSC1412 / ATCC 700720) (see 4 papers)
69% identity, 100% coverage: 1:260/260 of query aligns to 1:260/260 of P02911
- D33 (= D33) binding L-arginine; binding L-lysine; binding L-ornithine; mutation to A: Decreases arginine and histidine binding affinity.
- Y36 (= Y36) mutation to A: Shows a drastic decrease in arginine and histidine binding affinity.
- D52 (= D52) mutation to A: Shows a drastic decrease in arginine and histidine binding affinity.
- C60 (= C60) modified: Disulfide link with 67
- C67 (= C67) modified: Disulfide link with 60
- F74 (≠ L74) mutation to A: Shows a drastic decrease in arginine and histidine binding affinity.
- S91 (= S91) binding L-arginine; binding L-ornithine; mutation to A: Has no effect on the arginine and histidine binding affinity.
- S92 (= S92) binding L-arginine; binding L-lysine; binding L-ornithine; mutation to A: Decreases arginine and histidine binding affinity.
- S94 (= S94) binding L-arginine; binding L-lysine; binding L-ornithine; mutation to A: Decreases arginine and histidine binding affinity.
- R99 (= R99) binding L-arginine; binding L-lysine; binding L-ornithine; mutation to A: Shows a drastic decrease in arginine and histidine binding affinity.
- L139 (= L139) mutation to K: Changes ligand specificity. Can bind glutamine. Still able to bind basic amino acids, however, with 1000-fold less affinity for arginine.; mutation to Q: Does not affect ligand preference.
- T143 (= T143) binding L-arginine; binding L-lysine; binding L-ornithine
- D183 (= D183) binding L-arginine; binding L-ornithine; mutation to A: Shows a drastic decrease in arginine and histidine binding affinity.
1lstA Three-dimensional structures of the periplasmic lysine-, arginine-, ornithine-binding protein with and without a ligand (see paper)
71% identity, 92% coverage: 23:260/260 of query aligns to 1:238/238 of 1lstA
1lahE Structural bases for multiple ligand specificity of the periplasmic lysine-, arginine-, ornithine-binding protein (see paper)
71% identity, 92% coverage: 23:260/260 of query aligns to 1:238/238 of 1lahE
1lagE Structural bases for multiple ligand specificity of the periplasmic lysine-, arginine-, ornithine-binding protein (see paper)
71% identity, 92% coverage: 23:260/260 of query aligns to 1:238/238 of 1lagE
- binding histidine: Y14 (= Y36), F52 (≠ L74), S69 (= S91), S70 (= S92), L71 (= L93), S72 (= S94), R77 (= R99), L117 (= L139), S120 (≠ T142), Q122 (= Q144), D161 (= D183)
1lafE Structural bases for multiple ligand specificity of the periplasmic lysine-, arginine-, ornithine-binding protein (see paper)
71% identity, 92% coverage: 23:260/260 of query aligns to 1:238/238 of 1lafE
- binding arginine: D11 (= D33), Y14 (= Y36), F52 (≠ L74), S69 (= S91), S70 (= S92), S72 (= S94), R77 (= R99), L117 (= L139), S120 (≠ T142), T121 (= T143), Q122 (= Q144), D161 (= D183)
5owfA Structure of a lao-binding protein mutant with glutamine (see paper)
71% identity, 90% coverage: 26:260/260 of query aligns to 1:235/235 of 5owfA
4zv1A An ancestral arginine-binding protein bound to arginine (see paper)
37% identity, 87% coverage: 28:253/260 of query aligns to 6:225/226 of 4zv1A
- binding arginine: E11 (≠ D33), F14 (≠ Y36), F52 (≠ L74), A69 (≠ S91), G70 (≠ S92), M71 (≠ L93), T72 (≠ S94), R77 (= R99), Q117 (≠ L139), S120 (≠ T142), T121 (= T143), D161 (= D183)
4zv2A An ancestral arginine-binding protein bound to glutamine (see paper)
38% identity, 87% coverage: 28:253/260 of query aligns to 6:223/225 of 4zv2A
5otaA Structure of the periplasmic binding protein (pbp) noct from agrobacterium tumefaciens c58 in complex with octopinic acid (see paper)
37% identity, 89% coverage: 26:256/260 of query aligns to 2:251/254 of 5otaA
- binding (2~{S})-5-azanyl-2-[[(2~{R})-1-oxidanyl-1-oxidanylidene-propan-2-yl]amino]pentanoic acid: E9 (≠ D33), Y12 (= Y36), W50 (≠ L74), A68 (≠ S92), G70 (≠ S94), R75 (= R99), M90 (vs. gap), Q138 (≠ L139), T141 (= T142), S142 (≠ T143), H143 (≠ Q144), S180 (= S188)
5ot9A Structure of the periplasmic binding protein (pbp) noct from a.Tumefaciens c58 in complex with histopine. (see paper)
37% identity, 89% coverage: 26:256/260 of query aligns to 2:251/254 of 5ot9A
- binding Histopine: E9 (≠ D33), Y12 (= Y36), W50 (≠ L74), A67 (≠ S91), A68 (≠ S92), R75 (= R99), M90 (vs. gap), Q138 (≠ L139), T141 (= T142), S142 (≠ T143), H143 (≠ Q144), S180 (= S188)
4powA Structure of the pbp noct in complex with pyronopaline (see paper)
37% identity, 89% coverage: 26:256/260 of query aligns to 2:251/254 of 4powA
- binding 1-[(1S)-4-carbamimidamido-1-carboxybutyl]-5-oxo-D-proline: E9 (≠ D33), Y12 (= Y36), W50 (≠ L74), A67 (≠ S91), A68 (≠ S92), G70 (≠ S94), R75 (= R99), M90 (vs. gap), Q138 (≠ L139), T141 (= T142), S142 (≠ T143), H143 (≠ Q144), S180 (= S188)
5itoA Structure of the periplasmic binding protein m117n-noct from a. Tumefaciens in complex with octopine (see paper)
37% identity, 89% coverage: 26:256/260 of query aligns to 3:252/255 of 5itoA
- binding octopine: E10 (≠ D33), Y13 (= Y36), W51 (≠ L74), A68 (≠ S91), A69 (≠ S92), G71 (≠ S94), R76 (= R99), Q139 (≠ L139), T142 (= T142), S143 (≠ T143), H144 (≠ Q144), S181 (= S188)
5otcA Structure of the periplasmic binding protein (pbp) noct from agrobacterium tumefaciens c58 in complex with noroctopinic acid. (see paper)
37% identity, 89% coverage: 26:256/260 of query aligns to 2:251/256 of 5otcA
- binding (2~{S})-5-azanyl-2-(2-hydroxy-2-oxoethylamino)pentanoic acid: E9 (≠ D33), Y12 (= Y36), W50 (≠ L74), A68 (≠ S92), G70 (≠ S94), R75 (= R99), Q138 (≠ L139), T141 (= T142), S142 (≠ T143), H143 (≠ Q144), S180 (= S188)
5ovzA High resolution structure of the pbp noct in complex with nopaline (see paper)
37% identity, 89% coverage: 26:256/260 of query aligns to 3:252/259 of 5ovzA
- binding N-[(1S)-4-carbamimidamido-1-carboxybutyl]-D-glutamic acid: E10 (≠ D33), Y13 (= Y36), W51 (≠ L74), A68 (≠ S91), A69 (≠ S92), G71 (≠ S94), R76 (= R99), M91 (vs. gap), Q139 (≠ L139), T142 (= T142), S143 (≠ T143), H144 (≠ Q144), S181 (= S188), V213 (≠ T217)
2y7iA Structural basis for high arginine specificity in salmonella typhimurium periplasmic binding protein stm4351. (see paper)
35% identity, 87% coverage: 28:253/260 of query aligns to 8:227/228 of 2y7iA
- binding arginine: Y16 (= Y36), E20 (= E40), F54 (≠ L74), A71 (≠ S91), G72 (≠ S92), D74 (≠ S94), R79 (= R99), E118 (≠ L139), T121 (= T142), T122 (= T143), D160 (= D183)
- binding zinc ion: H9 (≠ R29), D23 (≠ N43), D25 (≠ Q45), D55 (= D75), D67 (= D87), D140 (≠ Q163)
8hqrA Crystal structure of the arginine-/lysine-binding protein sar11_1210 from 'candidatus pelagibacter ubique' htcc1062 bound to arginine
34% identity, 88% coverage: 27:256/260 of query aligns to 9:264/267 of 8hqrA
- binding arginine: E15 (≠ D33), Y18 (= Y36), W56 (≠ L74), A73 (≠ S91), G74 (≠ S92), S76 (= S94), R81 (= R99), E94 (≠ A112), Q143 (≠ L139), T146 (= T142), I147 (≠ T143)
6svfA Crystal structure of the p235gk mutant of argbp from t. Maritima (see paper)
35% identity, 86% coverage: 30:253/260 of query aligns to 14:227/229 of 6svfA
- binding arginine: F20 (≠ Y36), F58 (≠ L74), S75 (= S91), G76 (≠ S92), M77 (≠ L93), T78 (≠ S94), R83 (= R99), Q124 (≠ L139), T127 (= T142), T128 (= T143), D165 (= D183)
Query Sequence
>BWI76_RS20335 FitnessBrowser__Koxy:BWI76_RS20335
MKKLALSLSLALALSSVSTVFAAIPQKVRIGTDPTYAPFESKNAQGELVGFDIDLAKELC
KRINTQCTFIENPLDALIPSLKAKKIDAIMSSLSITEKRQQEIAFTDKLYAADSRLVVKK
GSDITPDLAKLKGKRVGVLQGTTQETYGNEHWAPKGIEIVSYQGQDNIYSDLTAGRIDAA
FQDEVAASEGFLKQPIGKDYQFGGPSIKDEKLFGVGTGMGLRKEDNELREALNKAFAEMR
QDGTYDKLAKKYFDFNVYGD
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SitesBLAST's Database
SitesBLAST's database includes
(1) SwissProt
entries with experimentally-supported functional features;
and (2) protein structures with bound ligands, from the
BioLip database.
by Morgan Price,
Arkin group
Lawrence Berkeley National Laboratory