SitesBLAST

SitesBLAST – Find functional sites

SitesBLAST

Comparing BWI76_RS20455 BWI76_RS20455 multifunctional fatty acid oxidation complex subunit alpha to proteins with known functional sites using BLASTp with E ≤ 0.001.

Or try Sites on a Tree, PaperBLAST, Conserved Domains, or compare to all protein structures

Found 20 (the maximum) hits to proteins with known functional sites (download)

6yswA E. Coli anaerobic trifunctional enzyme subunit-alpha in complex with coenzyme a
80% identity, 99% coverage: 4:707/714 of query aligns to 1:703/707 of 6yswA

query
sites
6yswA

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active site: A66 (= A69), I71 (= I74), A84 (= A87), Q88 (= Q91), G112 (= G115), E115 (= E118), P136 (= P139), E137 (= E140), G145 (= G148), D264 (≠ A267), S422 (= S425), H443 (= H446), E455 (= E458), N493 (= N496)
binding coenzyme a: E23 (= E26), M25 (= M28), T27 (= T30), A64 (= A67), A66 (= A69), D67 (= D70), I68 (= I71), N69 (= N72), P136 (= P139), E137 (= E140), L140 (= L143), F286 (= F289), F287 (= F290), T290 (= T293), K293 (= K296)

P40939 Trifunctional enzyme subunit alpha, mitochondrial; 78 kDa gastrin-binding protein; Monolysocardiolipin acyltransferase; TP-alpha; EC 2.3.1.-; EC 4.2.1.17; EC 1.1.1.211 from Homo sapiens (Human) (see 5 papers)
41% identity, 96% coverage: 15:700/714 of query aligns to 48:754/763 of P40939

query
sites
P40939

N

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E

R

R

F

F

-

G

S

G

P

G

P

N

A

P

N

E

I

L

I

L

S

T

S

Q

I

M

L

V

N

S

D

K

D

G

R

F

K

L

G

G

R

R

K

K

N

S

N

G

R

K

G

G

F

F

Y

Y

L

I

Y

Y

A

Q

A

E

K

G

G

V

R

K

K

R

S

K

K

D

K

L

R

N

P

S

D

D

-

M

P

D

A

S

I

I

Y

L

S

A

L

S

L

L

G

K

I

L

S

P

S

P

P

K

Q

S

A

E

R

V

L

S

S

S

E

D

Q

E

Q

D

V

I

A

Q

E

F

R

R

C

L

V

V

M

T

M

R

M

F

L

V

N

N

E

E

A

A

A

V

R

M

C

C

F

L

D

Q

E

E

R

G

V

I

V

L

R

A

S

T

A

P

R

A

D

E

G

G

D

D

I

I

G

G

A

A

V

V

F

F

G

G

I

L

G

G

F

F

P

P

P

P

F

C

L

L

G

G

G

G

P

P

F

F

R

R

Y

F

M

V

D

D

T

L

L

Y

G

G

A

A

G

Q

E

K

V

I

A

V

A

D

I

R

L

L

Q

K

R

K

L

Y

A

E

A

A

Q

A

Y

Y

G

G

P

K

R

Q

F

F

T

T

P

P

C

C

D

Q

T

L

L

L

L

A

H

D

M

H

A

A

V282 (≠ L228) to D: in MTPD; mild phenotype with slowly progressive myopathy and sensorimotor polyneuropathy; dbSNP:rs137852773
I305 (≠ L251) to N: in MTPD; mild phenotype with slowly progressive myopathy and sensorimotor polyneuropathy; dbSNP:rs137852774
L342 (≠ I288) to P: in LCHAD deficiency; dbSNP:rs137852772
E510 (= E458) active site, For hydroxyacyl-coenzyme A dehydrogenase activity; to Q: in AFLP and LCHAD deficiency; loss of long-chain-3-hydroxyacyl-CoA dehydrogenase activity; dbSNP:rs137852769

1wdlA Fatty acid beta-oxidation multienzyme complex from pseudomonas fragi, form ii (native4) (see paper)
35% identity, 98% coverage: 6:707/714 of query aligns to 7:714/715 of 1wdlA

query
sites
1wdlA

A

A

F

I

T

T

L

V

E

T

V

A

R

L

P

E

D

S

N

G

I

I

A

V

V

E

I

L

T

K

I

F

D

D

A

L

P

K

G

G

E

E

K

S

M

V

N

N

T

K

L

F

K

N

A

R

E

L

F

T

A

L

S

N

E

E

V

L

R

R

G

Q

I

A

I

V

R

D

Q

A

I

I

R

K

D

A

N

D

K

A

E

S

L

V

R

K

G

G

A

-

V

V

F

I

I

V

S

S

A

S

K

G

P

K

D

D

N

V

F

F

I

I

A

V

G

G

A
|
A

D

D

I

I

N

T

M

E

I

F

A

V

R

E

C

N

H

F

S

K

A

L

Q

P

E

D

A

A

E

E

A

L

L

I

A

A

-

G

-
x
N

R

L

Q

E

G

A

Q
x
N

Q

K

I

I

M

F

A

S

E

D

I

F

H

E

G

D

L

L

S

N

I

V

P

P

V

T

I

V

A

A

A

A

I

I

H

N

G

G

A

I

C

A

L

L

G

G

G
|
G

G

G

L

L

E
|
E

L

M

A

C

L

L

A

A

C

A

H

D

G

F

R

R

V

V

C

M

S

A

D

D

D

-

D

-

K

S

T

A

R

K

L

I

G

G

L

L

P
|
P

E
|
E

V

V

Q

K

L

L

G

G

L

I

L

Y

P
|
P

G
|
G

S

F

G

G

G

G

T

T

Q

V

R

R

L

L

P

P

R

R

L

L

I

I

G

G

V

V

S

D

G

N

A

A

L

V

E

E

M

W

I

I

L

A

T

S

G

G

K

K

Q

E

L

N

R

R

P

A

R

E

Q

D

A

A

L

L

K

K

A

V

G

S

L

A

V

V

D

D

E

A

V

V

V

V

A

T

Q

A

T

D

I

K

L

L

L

G

Q

A

T

A

A

A

V

L

E

D

L

L

-

I

-

K

-

R

A

A

L

I

K

S

G

G

R

E

P

L

A

D

S

Y

R

K

A

A

V

K

-

R

-

Q

P

P

A

K

R

L

E

E

R

K

I

L

L

K

A

L

G

N

P

A

L

I

G

E

R

Q

S

M

L

M

L

A

F

F

R

E

L

T

V

A

A

K

K

G

K

F

T

V

H

A

H

G

K

Q

T

A

Q

G

G

P

N

N

Y

Y

P

P

A

A

T

P

E

V

R

E

I

A

L

I

Q

K

V

T

M

I

E

Q

T

K

G

A

L

A

A

N

Q

F

G

G

C

R

S

D

S

K

G

A

Y

L

A

E

E

V

E

E

A

A

R

A

A

G

F

F

G

A

E

K

L

L

A

A

M

K

T

T

P

S

Q

A

S

S

Q

N

A

C

L

L

R

I

S

G

I

L

F

F

F

L

A

N

S

D

T

Q

D

E

L

L

K

K

K

K

D

K

-

A

R

K

G

V

A

Y

D

D

A

K

E

I

P

A

V

K

A

D

L

V

R

K

S

Q

V

A

G

A

I

V

L

L

G
|
G

G
x
A

G

G

L
x
I

M
|
M

G

G

G

G

G

G

I

I

A

A

Y

Y

V

Q

T

S

A

A

C

S

K

K

G

G

G

-

L

T

P

P

V

I

R

L

I

M

K

K

D
|
D

I
|
I

Q

N

P

E

R

H

G

G

I

I

N

E

H

Q

A

G

L

L

K

A

Y

E

S

A

W

A

D

K

L

L

L

L

D

V

K

G

Q

R

V

V

R

D

R

K

R

G

Y

R

L

M

R

T

A

P

S

A

E

K

R

M

D

A

R

E

Q

V

I

L

G

N

L

G

I

I

S

R

G

P

S

T

L

L

D

S

Y

Y

Q

G

G

D

F

F

A

G

H

N

R

V

D

D

V

L

V

V

I

V

E

E

A
|
A

V
|
V

F
x
V

E
|
E

D

N

L

P

A

K

L

V

K
|
K

Q

Q

K

A

M
x
V

V

L

S

A

E

E

V

V

E

E

Q

N

H

H

C

V

R

R

P

E

E

D

T

A

I

I

F

L

A

A

S

S

N
|
N

T
|
T

S
|
S

S

T

L

I

P

S

I

I

G

S

E

L

I

L

A

A

A

K

Q

A

A

L

S

K

R

R

P

P

Q

E

R

N

V

F

I

V

G

G

L

M

H
|
H

F

F

F

F

S

N

P

P

V

V

D

H

K

M

M

M

P

P

L

L

V

V

E
|
E

V

V

I

I

P

R

H

G

I

E

G

K

T

S

D

S

R

D

Q

L

T

A

I

V

A

A

T

T

A

T

V

V

K

A

L

Y

A

A

K

K

L

K

Q

M

G

G

K

K

T

N

P

P

I

I

V

V

V

V

A

N

D

D

K

C

A

P

G

G

F

F

Y

L

V

V

N
|
N

R

R

I

V

L

L

A

F

P

P

Y

Y

I

F

N

G

E

G

A

F

M

A

R

K

L

L

L

V

M

S

E

A

G

G

E

V

P

D

V

F

E

V

H

R

I

I

D

D

N

K

A

V

L

M

V

E

K

K

F

F

G

G

F

W

P

P

V

M

G

G

P

P

I

A

Q

Y

L

L

L

M

D

D

E

V

V

V

G

G

I

I

D

D

T

T

G

G

T

H

K

H

I

G

I

R

P

D

V

V

L

M

E

A

A

E

A

G

W

F

G

P

E

D

R

R

F

M

S

K

P

D

P

D

-

R

A

R

N

S

I

A

I

I

S

D

S

A

I

L

L

Y

N

E

D

A

D

K

R

R

K

L

G

G

R

Q

K

K

N

N

N

G

R

K

G

G

F

F

Y

Y

L

A

Y

Y

A

E

A

A

-

D

K

K

G

K

R

G

K

K

S

Q

K

K

K

K

R

L

P

V

D

D

P

S

A

S

I

V

Y

L

S

E

L

V

L

L

G

K

-

P

I

I

S

V

S

Y

P

E

Q

Q

A

R

R

D

L

V

S

T

E

D

Q

E

Q

D

V

I

A

I

E

N

R

W

C

M

V

M

M

I

M

P

M

L

L

C

N

L

E

E

A

T

A

V

R

R

C

C

F

L

D

E

E

D

R

G

V

I

V

V

R

E

S

T

A

A

R

A

D

E

G

A

D

D

I

M

G

G

A

L

V

V

F

Y

G

G

I

I

G

G

F

F

P

P

P

L

F

F

L

R

G

G

G

G

P

A

F

L

R

R

Y

Y

M

I

D

D

T

S

L

I

G

G

A

V

G

A

E

E

V

F

A

V

A

A

I

L

L

A

Q

D

R

Q

L

Y

A

-

A

A

Q

E

Y

L

G

G

P

A

R

L

F

Y

T

H

P

P

C

T

D

A

T

K

L

L

L

R

H

E

M

M

A

A

E

K

Q

N

G

G

A

Q

T

S

F

F

W

F

active site: A69 (= A69), N89 (vs. gap), N93 (≠ Q91), G117 (= G115), E120 (= E118), P139 (= P139), E140 (= E140), P147 (= P147), G148 (= G148), S430 (= S425), H451 (= H446), E463 (= E458), N501 (= N496)
binding nicotinamide-adenine-dinucleotide: G321 (= G315), A322 (≠ G316), I324 (≠ L318), M325 (= M319), D344 (= D339), I345 (= I340), A400 (= A395), V401 (= V396), V402 (≠ F397), E403 (= E398), K408 (= K403), V411 (≠ M406), N428 (= N423), T429 (= T424), S430 (= S425)

P28793 Fatty acid oxidation complex subunit alpha; EC 4.2.1.17; EC 5.1.2.3; EC 5.3.3.8; EC 1.1.1.35 from Pseudomonas fragi (see paper)
35% identity, 98% coverage: 6:707/714 of query aligns to 7:714/715 of P28793

query
sites
P28793

A

A

F

I

T

T

L

V

E

T

V

A

R

L

P

E

D

S

N

G

I

I

A

V

V

E

I

L

T

K

I

F

D

D

A

L

P

K

G

G

E

E

K

S

M

V

N

N

T

K

L

F

K

N

A

R

E

L

F

T

A

L

S

N

E

E

V

L

R

R

G

Q

I

A

I

V

R

D

Q

A

I

I

R

K

D

A

N

D

K

A

E

S

L

V

R

K

G

G

A

-

V

V

F

I

I

V

S

S

A

S

K

G

P

K

D

D

N

V

F

F

I

I

A

V

G

G

A

A

D

D

I

I

N

T

M

E

I

F

A

V

R

E

C

N

H

F

S

K

A

L

Q

P

E

D

A

A

E

E

A

L

L

I

A

A

-

G

-

N

R

L

Q

E

G

A

Q

N

Q

K

I

I

M

F

A

S

E

D

I

F

H

E

G

D

L

L

S

N

I

V

P

P

V

T

I

V

A

A

A

A

I

I

H

N

G

G

A

I

C

A

L

L

G

G

G

G

G

G

L

L

E

E

L

M

A

C

L

L

A

A

C

A

H

D

G

F

R

R

V

V

C

M

S

A

D

D

D

-

D

-

K

S

T

A

R

K

L

I

G

G

L

L

P

P

E

E

V

V

Q

K

L

L

G

G

L

I

L

Y

P

P

G

G

S

F

G

G

G

G

T

T

Q

V

R

R

L

L

P

P

R

R

L

L

I

I

G

G

V

V

S

D

G

N

A

A

L

V

E

E

M

W

I

I

L

A

T

S

G

G

K

K

Q

E

L

N

R

R

P

A

R

E

Q

D

A

A

L

L

K

K

A

V

G

S

L

A

V

V

D

D

E

A

V

V

V

V

A

T

Q

A

T

D

I

K

L

L

L

G

Q

A

T

A

A

A

V

L

E

D

L

L

-

I

-

K

-

R

A

A

L

I

K

S

G

G

R

E

P

L

A

D

S

Y

R

K

A

A

V

K

-

R

-

Q

P

P

A

K

R

L

E

E

R

K

I

L

L

K

A

L

G

N

P

A

L

I

G

E

R

Q

S

M

L

M

L

A

F

F

R

E

L

T

V

A

A

K

K

G

K

F

T

V

H

A

H

G

K

Q

T

A

Q

G

G

P

N

N

Y

Y

P

P

A

A

T

P

E

V

R

E

I

A

L

I

Q

K

V

T

M

I

E

Q

T

K

G

A

L

A

A

N

Q

F

G

G

C

R

S

D

S

K

G

A

Y

L

A

E

E

V

E

E

A

A

R

A

A

G

F

F

G

A

E

K

L

L

A

A

M

K

T

T

P

S

Q

A

S

S

Q

N

A

C

L

L

R

I

S

G

I

L

F

F

F

L

A

N

S
x
D

T

Q

D

E

L

L

K

K

K

K

D

K

-

A

R

K

G

V

A

Y

D

D

A

K

E

I

P

A

V

K

A

D

L

V

R

K

S

Q

V

A

G

A

I

V

L

L

G

G

G

A

G

G

L

I

M
|
M

G

G

G

G

G

G

I

I

A

A

Y

Y

V

Q

T

S

A

A

C

S

K

K

G

G

G

-

L

T

P

P

V

I

R

L

I

M

K

K

D
|
D

I

I

Q

N

P

E

R

H

G

G

I

I

N

E

H

Q

A

G

L

L

K

A

Y

E

S

A

W

A

D

K

L

L

L

L

D

V

K

G

Q

R

V

V

R

D

R

K

R

G

Y

R

L

M

R

T

A

P

S

A

E

K

R

M

D

A

R

E

Q

V

I

L

G

N

L

G

I

I

S

R

G

P

S

T

L

L

D

S

Y

Y

Q

G

G

D

F

F

A

G

H

N

R

V

D

D

V

L

V

V

I

V

E

E

A

A

V
|
V

F
x
V

E
|
E

D

N

L

P

A

K

L

V

K
|
K

Q

Q

K

A

M

V

V

L

S

A

E

E

V

V

E

E

Q

N

H

H

C

V

R

R

P

E

E

D

T

A

I

I

F

L

A

A

S

S

N

N

T

T

S
|
S

S

T

L

I

P

S

I

I

G

S

E

L

I

L

A

A

A

K

Q

A

A

L

S

K

R

R

P

P

Q

E

R

N

V

F

I

V

G

G

L

M

H

H

F

F

F

F

S
x
N

P

P

V

V

D

H

K

M

M

M

P

P

L

L

V

V

E

E

V

V

I

I

P

R

H

G

I

E

G

K

T

S

D

S

R

D

Q

L

T

A

I

V

A

A

T

T

A

T

V

V

K

A

L

Y

A

A

K

K

L

K

Q

M

G

G

K

K

T

N

P

P

I

I

V

V

V

V

A

N

D

D

K

C

A

P

G

G

F

F

Y

L

V

V

N
|
N

R

R

I

V

L

L

A

F

P

P

Y

Y

I

F

N

G

E

G

A

F

M

A

R

K

L

L

L

V

M

S

E

A

G

G

E

V

P

D

V

F

E

V

H

R

I

I

D

D

N

K

A

V

L

M

V

E

K

K

F

F

G

G

F

W

P

P

V

M

G

G

P

P

I

A

Q

Y

L

L

L

M

D

D

E

V

V

V

G

G

I

I

D

D

T

T

G

G

T

H

K

H

I

G

I

R

P

D

V

V

L

M

E

A

A

E

A

G

W

F

G

P

E

D

R

R

F

M

S

K

P

D

P

D

-

R

A

R

N

S

I

A

I

I

S

D

S

A

I

L

L

Y

N

E

D

A

D

K

R

R

K

L

G

G

R

Q

K

K

N

N

N

G

R

K

G

G

F

F

Y

Y

L

A

Y

Y

A

E

A

A

-

D

K

K

G

K

R

G

K

K

S

Q

K

K

K

K

R

L

P

V

D

D

P

S

A

S

I

V

Y

L

S

E

L

V

L

L

G

K

-

P

I

I

S

V

S

Y

P

E

Q

Q

A

R

R

D

L

V

S

T

E

D

Q

E

Q

D

V

I

A

I

E

N

R

W

C

M

V

M

M

I

M

P

M

L

L

C

N

L

E

E

A

T

A

V

R

R

C

C

F

L

D

E

E

D

R

G

V

I

V

V

R

E

S

T

A

A

R

A

D

E

G

A

D

D

I

M

G

G

A

L

V

V

F
x
Y

G

G

I

I

G

G

F

F

P

P

P

L

F

F

L

R

G

G

G

G

P

A

F

L

R

R

Y

Y

M

I

D

D

T

S

L

I

G

G

A

V

G

A

E

E

V

F

A

V

A

A

I

L

L

A

Q

D

R

Q

L

Y

A

-

A

A

Q

E

Y

L

G

G

P

A

R

L

F

Y

T

H

P

P

C

T

D

A

T

K

L

L

L

R

H

E

M

M

A

A

E

K

Q

N

G

G

A

Q

T

S

F

F

W

F

D297 (≠ S292) binding
M325 (= M319) binding
D344 (= D339) binding
VVE 401:403 (≠ VFE 396:398) binding
K408 (= K403) binding
S430 (= S425) binding
N454 (≠ S449) binding
N501 (= N496) binding
Y660 (≠ F652) binding

P21177 Fatty acid oxidation complex subunit alpha; EC 4.2.1.17; EC 5.1.2.3; EC 5.3.3.8; EC 1.1.1.35 from Escherichia coli (strain K12) (see 2 papers)
35% identity, 98% coverage: 9:711/714 of query aligns to 10:718/729 of P21177

query
sites
P21177

L

L

E

D

V

W

R

L

P

E

D

D

N

G

I

I

A

A

V

E

I

L

T

V

I

F

D

D

A

A

P

P

G

G

-

S

-

V

E

N

K

K

M

L

N

D

T

T

L

A

K

T

A

V

E

A

F

S

A

L

S

G

E

E

V

A

R

I

G

G

I

V

I

L

R

E

Q

Q

I

Q

R

S

D

D

N

-

K

-

E

-

L

L

R

K

G

G

A

L

V

L

F

L

I

R

S

S

A

N

K

K

P

A

D

-

N

A

F

F

I

I

A

V

G

G

A

A

D

D

I

I

N

T

M

E

I

F

A

L

R

S

C

L

H

F

S

L

A

V

Q

P

E

E

A

-

E

E

A

Q

L

L

A

S

R

Q

Q

W

-

L

-

H

-

F

G

A

Q

N

Q

S

I

V

M

F

A

N

E

R

I

L

H

E

G

D

L

L

S

P

I

V

P

P

V

T

I

I

A

A

A

A

I

V

H

N

G

G

A

Y

C

A

L

L

G

G

G
|
G

G

G

L

C

E

E

L

C

A

V

L

L

A

A

C

T

H

D

G

Y

R

R

V

L

C

A

S

T

D

P

D

D

D

-

K

-

T

L

R

R

L

I

G

G

L

L

P

P

E

E

V

T

Q

K

L

L

G

G

L

I

L

M

P

P

G

G

S

F

G

G

G

G

T

S

Q

V

R

R

L

M

P

P

R

R

L

M

I

L

G

G

V

A

S

D

G

S

A

A

L

L

E

E

M

I

I

I

L

A

T

A

G

G

K

K

Q

D

L

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R

G

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A

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Q

Q

A

A

L

L

K

K

A

I

G

G

L

L

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V

D

D

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G

V

V

V

V

A

K

Q

A

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K

L

L

L

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A

A

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K

-

A

-

V

V

L

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L

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A

A

L

I

K

N

G

G

R

D

P

L

A

D

S

W

R

K

A

A

V

K

-

R

-

Q

P

P

A

K

R

L

E

E

R

P

I

L

L

K

A

L

G

S

P

K

L

I

G

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R

A

S

T

L

M

L

S

F

F

R

T

L

I

V

A

A

K

K

G

K

M

T

V

H

A

H

Q

K

T

T

A

Q

G

G

K

N

H

Y

Y

P

P

A

A

T

P

E

I

R

T

I

A

L

V

Q

K

V

T

M

I

E

E

T

A

G

A

L

A

A

R

Q

F

G

G

C

R

S

E

S

E

G

A

Y

L

A

N

E

L

E

E

A

N

R

K

A

S

F

F

G

V

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P

L

L

A

A

M

H

T

T

P

N

Q

E

S

A

Q

R

A

A

L

L

R

V

S

G

I

I

F

F

F

L

A

N

S

D

T

Q

D

Y

L

V

K

K

-

G

K

K

D

A

R

K

G

K

A

L

D

T

A

K

E

D

P

V

V

E

A

T

L

P

R

K

S

Q

V

A

G

A

I

V

L

L

G

G

G

A

G
|
G

L

I

M

M

G

G

G

G

G

G

I

I

A

A

Y

Y

V

Q

T

S

A

A

C

W

K

K

G

G

G

-

L

V

P

P

V

V

R

V

I

M

K

K

D

D

I

I

Q

N

P

D

R

K

G

S

I

L

N

T

H

L

A

G

L

M

K

T

Y

E

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A

W

A

D

K

L

L

L

L

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N

K

K

Q

Q

V

L

R

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R

R

R

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Y

K

L

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D

A

G

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L

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K

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L

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A

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G

Q

V

I

I

G

S

L

T

I

I

S

H

G

P

S

T

L

L

D

D

Y

Y

Q

A

G

G

F

F

A

D

H

R

R

V

D

D

V

I

V

V

I

V

E

E

A

A

V

V

F

V

E

E

D

N

L

P

A

K

L

V

K

K

Q

K

K

A

M

V

V

L

S

A

E

E

V

T

E

E

Q

Q

H

K

C

V

R

R

P

Q

E

D

T

T

I

V

F

L

A

A

S

S

N

N

T

T

S

S

S

T

L

I

P

P

I

I

G

S

E

E

I

L

A

A

A

N

Q

A

A

L

S

E

R

R

P

P

Q

E

R

N

V

F

I

C

G

G

L

M

H
|
H

F

F

F

F

S

N

P

P

V

V

D

H

K

R

M

M

P

P

L

L

V

V

E

E

V

I

I

I

P

R

H

G

I

E

G

K

T

S

D

S

R

D

Q

E

T

T

I

I

A

A

T

K

A

V

V

V

K

A

L

W

A

A

K

S

L

K

Q

M

G

G

K

K

T

T

P

P

I

I

V

V

V

V

A

N

D

D

K

C

A

P

G

G

F

F

Y

F

V

V

N

N

R

R

I

V

L

L

A

F

P

P

Y

Y

I

F

N

A

E

G

A

F

M

S

R

Q

L

L

L

L

M

R

E

D

G

G

E

A

P

D

V

F

E

R

H

K

I

I

D

D

N

K

A

V

L

M

V

E

K

K

-

Q

F

F

G

G

F

W

P

P

V

M

G

G

P

P

I

A

Q

Y

L

L

L

L

D

D

E

V

V

V

G

G

I

I

D

D

T

T

G

A

T

H

K

H

I

A

I

Q

P

A

V

V

L

M

E

A

A

A

A

G

W

F

G

P

E

Q

R

R

F

M

S

Q

P

K

P

D

A

Y

-

R

N

D

I

A

I

I

S

D

S

A

I

L

L

F

N

D

D

A

D

N

R

R

K

F

G

G

R

Q

K

K

N

N

N

G

R

L

G

G

F

F

Y

W

L

R

Y

Y

A

K

A

E

K

D

G

S

R

K

-

G

K

K

S

P

K

K

K

K

R

E

P

E

D

D

P

A

A

A

I

V

Y

E

S

D

L

L

L

L

G

A

-

E

I

V

S

S

S

Q

P

P

Q

K

A

R

R

D

L

F

S

S

E

E

Q

E

Q

E

V

I

A

I

E

A

R

R

C

M

V

M

M

I

M

P

M

M

L

V

N

N

E

E

A

V

A

V

R

R

C

C

F

L

D

E

E

E

R

G

V

I

V

I

R

A

S

T

A

P

R

A

D

E

G

A

D

D

I

M

G

A

A

L

V

V

F

Y

G

G

I

L

G

G

F

F

P

P

P

P

F

F

L

H

G

G

G

G

P

A

F

F

R

R

Y

W

M

L

D

D

T

T

L

L

G

G

A

S

G

A

E

K

V

Y

A

L

A

D

I

M

L

A

Q

Q

R

Q

L

Y

A

-

A

Q

Q

H

Y

L

G

G

P

P

R

L

F

Y

T

E

P

V

C

P

D

E

T

G

L

L

L

R

H

N

M

K

A

A

E

R

Q

H

G

N

A

E

T

P

F

Y

W

Y

P

P

A

P

E

V

E

E

G116 (= G115) mutation to F: Absence of both enoyl-CoA hydratase and 3-hydroxyacyl-CoA epimerase activities. Delta(3)-cis-Delta(2)-trans-enoyl-CoA isomerase is only slightly affected.
G322 (= G317) mutation to A: 10-fold increase in KM for NADH.
H450 (= H446) active site, For 3-hydroxyacyl-CoA dehydrogenase activity; mutation H->A,Q: Almost complete loss of 3-hydroxyacyl-CoA dehydrogenase activity.

6tnmA E. Coli aerobic trifunctional enzyme subunit-alpha (see paper)
35% identity, 98% coverage: 9:711/714 of query aligns to 10:718/719 of 6tnmA

query
sites
6tnmA

L

L

E

D

V

W

R

L

P

E

D

D

N

G

I

I

A

A

V

E

I

L

T

V

I

F

D

D

A

A

P

P

G

G

-

S

-

V

E

N

K

K

M

L

N

D

T

T

L

A

K

T

A

V

E

A

F

S

A

L

S

G

E

E

V

A

R

I

G

G

I

V

I

L

R

E

Q

Q

I

Q

R

S

D

D

N

-

K

-

E

-

L

L

R

K

G

G

A

L

V

L

F

L

I

R

S

S

A

N

K

K

P

A

D

-

N

A

F

F

I

I

A

V

G

G

A
|
A

D

D

I

I

N

T

M

E

I
x
F

A

L

R

S

C

L

H

F

S

L

A

V

Q

P

E

E

A

-

E

E

A

Q

L

L

A

S

R

Q

Q

W

-

L

-

H

-

F

G

A

Q

N

Q

S

I

V

M

F

A

N

E

R

I

L

H

E

G

D

L

L

S

P

I

V

P

P

V

T

I

I

A

A

A

A

I

V

H

N

G

G

A

Y

C

A

L

L

G

G

G
|
G

G

G

L

C

E
|
E

L

C

A

V

L

L

A

A

C

T

H

D

G

Y

R

R

V

L

C

A

S

T

D

P

D

D

D

-

K

-

T

L

R

R

L

I

G

G

L

L

P
|
P

E
|
E

V

T

Q

K

L

L

G

G

L

I

L

M

P

P

G
|
G

S

F

G

G

G

G

T

S

Q

V

R

R

L

M

P

P

R

R

L

M

I

L

G

G

V

A

S

D

G

S

A

A

L

L

E

E

M

I

I

I

L

A

T

A

G

G

K

K

Q

D

L

V

R

G

P

A

R

D

Q

Q

A

A

L

L

K

K

A

I

G

G

L

L

V

V

D

D

E

G

V

V

V

V

A

K

Q

A

T

E

I

K

L

L

L

V

Q

E

T

G

A

A

-

K

-

A

-

V

V

L

E

R

L

Q

A

A

L

I

K

N

G

G

R

D

P

L

A

D

S

W

R

K

A

A

V

K

-

R

-

Q

P

P

A

K

R

L

E

E

R

P

I

L

L

K

A

L

G

S

P

K

L

I

G

E

R

A

S

T

L

M

L

S

F

F

R

T

L

I

V

A

A

K

K

G

K

M

T

V

H

A

H

Q

K

T

T

A

Q

G

G

K

N

H

Y

Y

P

P

A

A

T

P

E

I

R

T

I

A

L

V

Q

K

V

T

M

I

E

E

T

A

G

A

L

A

A

R

Q

F

G

G

C

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A

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x
N

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|
D

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V

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x
V

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x
V

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|
S

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L

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A

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T

T

L

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G

G

A

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Y

A

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A

D

I

M

L

A

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Q

R

Q

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Y

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-

A

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Y

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G

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Y

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L

L

L

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N

M

K

A

A

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Q

H

G

N

A

E

T

P

F

Y

W

Y

P

P

A

P

E

V

E

E

active site: A68 (= A69), F73 (≠ I74), G116 (= G115), E119 (= E118), P138 (= P139), E139 (= E140), G147 (= G148), N271 (≠ A267), S429 (= S425), H450 (= H446), E462 (= E458), N500 (= N496)
binding adenosine-5'-triphosphate: D343 (= D339), I344 (= I340), V400 (= V396), V401 (≠ F397), V406 (≠ L402), K584 (= K578)

1wdmA Fatty acid beta-oxidation multienzyme complex from pseudomonas fragi, form i (native3) (see paper)
35% identity, 98% coverage: 6:707/714 of query aligns to 7:706/707 of 1wdmA

query
sites
1wdmA

A

A

F

I

T

T

L

V

E

T

V

A

R

L

P

E

D

S

N

G

I

I

A

V

V

E

I

L

T

K

I

F

D

D

A

L

P

K

G

G

E

E

K

S

M

V

N

N

T

K

L

F

K

N

A

R

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L

F

T

A

L

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N

E

E

V

L

R

R

G

Q

I

A

I

V

R

D

Q

A

I

I

R

K

D

A

N

D

K

A

E

S

L

V

R

K

G

G

A

-

V

V

F

I

I

V

S

S

A

S

K

G

P

K

D

D

N

V

F

F

I

I

A

V

G

G

A
|
A

D

D

I

I

N

T

M

E

I

F

A

V

R

E

C

N

H

F

S

K

A

L

Q

P

E

D

A

A

E

E

A

L

L

I

A

A

-

G

-
x
N

R

L

Q

E

G

A

Q
x
N

Q

K

I

I

M

F

A

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D

I

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D

L

L

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N

I

V

P

P

V

T

I

V

A

A

A

A

I

I

H

N

G

G

A

I

C

A

L

L

G

G

G
|
G

G

G

L

L

E
|
E

L

M

A

C

L

L

A

A

C

A

H

D

G

F

R

R

V

V

C

M

S

A

D

D

D

-

D

-

K

S

T

A

R

K

L

I

G

G

L

L

P
|
P

E
|
E

V

V

Q
x
K

L

L

G

G

L

I

L

Y

P
|
P

G
|
G

S

F

G

G

G

G

T

T

Q

V

R

R

L

L

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P

R

R

L

L

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I

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G

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G

N

A

A

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M

W

I

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G

G

K

K

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A

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K

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D

D

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V

V

V

A

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Q

A

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K

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L

L

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A

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A

A

A

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L

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D

L

L

-

I

-

K

-

R

A

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G

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L

A

D

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A

A

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K

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R

-

Q

P

P

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K

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L

L

K

A

L

G

N

P

A

L

I

G

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R

Q

S

M

L

M

L

A

F

F

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L

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A

A

K

K

G

K

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H

A

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G

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N

N

Y

Y

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A

L

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A

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A

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G

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K

G

A

Y

L

A

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E

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A

A

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A

A

G

F

F

G

A

E

K

L

L

A

A

M

K

T

T

P

S

Q

A

S

S

Q

N

A

C

L

L

R

I

S

G

I

L

F

F

F

L

A

N

S
x
D

T

Q

D

E

L

L

K

K

K

K

D

K

-

A

R

K

G

V

A

Y

D

D

A

K

E

I

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A

V

K

A

D

L

V

R

K

S

Q

V

A

G

A

I

V

L

L

G
|
G

G
x
A

G

G

L
x
I

M
|
M

G

G

G

G

G

G

I

I

A

A

Y

Y

V

Q

T

S

A

A