SitesBLAST

SitesBLAST – Find functional sites

SitesBLAST

Comparing BWI76_RS20605 FitnessBrowser__Koxy:BWI76_RS20605 to proteins with known functional sites using BLASTp with E ≤ 0.001.

Or try Sites on a Tree, PaperBLAST, Conserved Domains, or compare to all protein structures

Found 20 (the maximum) hits to proteins with known functional sites (download)

P45563 Purine nucleoside phosphorylase 2; Inosine-guanosine phosphorylase; Purine nucleoside phosphorylase II; PNP II; Xanthosine phosphorylase; EC 2.4.2.1 from Escherichia coli (strain K12) (see paper)
88% identity, 100% coverage: 1:277/277 of query aligns to 1:277/277 of P45563

query
sites
P45563

M

M

S

S

D

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V

L

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A

H65 (= H65) binding
RGH 85:87 (= RGH 85:87) binding
A117 (= A117) binding
Y191 (= Y191) mutation to L: No detectable activity with xanthosine as substrate, but largely retains its activity against other substrates, namely inosine and guanosine, although with altered affinities, higher and lower respectively, and clearly reduced maximal velocities for both.
E197 (= E197) binding
S216 (= S216) binding
N239 (= N239) binding ; mutation to D: Catalyzes the phosphorolysis of adenosine with moderate efficiency, and essentially has lost all activity against the 6-oxo-purine substrates xanthosine, inosine and guanosine.

1yr3A Escherichia coli purine nucleoside phosphorylase ii, the product of the xapa gene (see paper)
89% identity, 98% coverage: 6:277/277 of query aligns to 2:273/273 of 1yr3A

query
sites
1yr3A

F

F

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S

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H

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N

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active site: S30 (= S34), H61 (= H65), H83 (= H87), Y85 (= Y89), E86 (= E90), A113 (= A117), M211 (= M215), S212 (= S216), N235 (= N239), A237 (= A241), H247 (= H251)
binding xanthine: A114 (= A118), E193 (= E197), G210 (= G214), N235 (= N239)

1yqqA Escherichia coli purine nucleoside phosphorylase ii, the product of the xapa gene (see paper)
89% identity, 98% coverage: 6:277/277 of query aligns to 2:273/273 of 1yqqA

query
sites
1yqqA

F

F

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S

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A

active site: S30 (= S34), H61 (= H65), H83 (= H87), Y85 (= Y89), E86 (= E90), A113 (= A117), M211 (= M215), S212 (= S216), N235 (= N239), A237 (= A241), H247 (= H251)
binding guanine: A114 (= A118), G115 (= G119), E193 (= E197), G210 (= G214), M211 (= M215), T234 (= T238), N235 (= N239), T250 (= T254)
binding phosphate ion: H61 (= H65), R81 (= R85), H83 (= H87), N112 (= N116), S212 (= S216)

3odgA Crystal structure of xanthosine phosphorylase bound with xanthine from yersinia pseudotuberculosis
69% identity, 98% coverage: 6:277/277 of query aligns to 2:273/273 of 3odgA

query
sites
3odgA

F

F

S

N

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E

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F

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A

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S

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A

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E

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D

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C

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M

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G

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M

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G

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P

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N

D

D

E

D

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F

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A

A

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T

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L

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A

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H

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A

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A

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E

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N

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K

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L

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A

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F

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A

A

active site: S30 (= S34), H61 (= H65), H83 (= H87), Y85 (= Y89), E86 (= E90), A113 (= A117), M211 (= M215), S212 (= S216), N235 (= N239), A237 (= A241), H247 (= H251)
binding xanthine: A114 (= A118), G115 (= G119), F192 (= F196), E193 (= E197), G210 (= G214), M211 (= M215), T234 (= T238), N235 (= N239)

P46354 Purine nucleoside phosphorylase 1; PNP 1; Inosine phosphorylase; Inosine-guanosine phosphorylase; Purine nucleoside phosphorylase I; PNP I; Pu-NPase I; EC 2.4.2.1 from Bacillus subtilis (strain 168) (see paper)
45% identity, 93% coverage: 13:269/277 of query aligns to 8:263/271 of P46354

query
sites
P46354

S

A

A

A

D

A

I

F

I

I

R

K

G

Q

Y

N

K

L

P

P

D

E

F

-

T

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G

F

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I

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L

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G

S
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S

G

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L

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G

A

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D

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E

I

I

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E

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K

Y

Y

E

E

K

D

L

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P

P

G

E

F

F

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T

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H

E

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H

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F

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E

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K

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A

L

L

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G

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E

E

L

A

L

L

F

I

C

V

T

T

N

N

A

A

G

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S

G

L

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R

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P

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E

E

V

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R

P

A

G

G

S

D

L

L

V

M

A

I

L

I

S

T

D

D

H

H

I

I

N

N

T

F

M

M

P

G

G

T

T

N

P

P

M

L

V

I

G

G

P

P

N

N

D

E

E

A

R

D

F

F

G

G

E

A

R

R

F

F

F

P

S

D

L

M

A

S

N

S

A

A

Y

Y

D

D

A

K

D

D

Y

L

R

S

A

S

V

L

L

A

Q

E

S

K

V

I

A

A

A

K

E

D

E

L

G

N

F

I

P

P

L

I

T

Q

E

K

G

G

V

V

F

Y

V

T

S

A

Y

V

P

T

G

G

P

P

N

S

F

Y

E

E

T

T

A

P

A

A

E

E

I

V

R

R

M

F

M

L

Q

R

I

T

I

M

G

G

G

S

D

D

V

A

V

V

G

G

M

M

S

S

V

T

V

V

P

P

E

E

V

V

I

I

T

V

A

A

R

N

H

H

C

A

G

G

L

M

K

R

V

V

V

L

A

G

V

I

S

S

A

C

I

I

T

S

N

N

L

A

A

A

E

A

G

G

L

I

G

L

D

D

V

Q

K

P

L

L

S

S

H

H

A

D

Q

E

T

V

L

M

A

E

A

V

A

T

E

E

L

K

S

V

R

K

Q

A

N

G

F

F

I

L

N

K

L

L

I

V

S28 (= S34) modified: Phosphoserine

8swuA Structure of clostridium perfringens pnp bound to transition state analog immucillin h and sulfate (see paper)
44% identity, 95% coverage: 13:276/277 of query aligns to 11:269/269 of 8swuA

query
sites
8swuA

S

A

A

A

D

E

I

Y

I

I

R

K

G

G

Y

-

K

K

P

S

D

K

F

Y

T

N

P

P

R

T

V

I

A

G

F

L

I

I

L

L

G

G

S

S

G

G

L

L

G

G

A

A

L

I

A

A

E

D

Q

Q

I

I

E

E

D

D

A

A

V

E

A

Y

I

F

S

P

Y

Y

E

N

K

E

L

I

P

P

G

N

F

F

P

P

V

V

S

S

T

T

V

-

H

-

G

-

H

-

A

A

G

G

E

R

L

L

V

V

L

I

G

G

N

K

L

F

A

Q

G

G

V

K

P

E

V

V

A

V

C

A

M

M

K

Q

G

G

R

R

G

F

H

H

F

Y

Y
|
Y

E

E

G

G

R

Y

G

S

M

M

P

Q

V

E

M

V

T

T

D

C

A

P

I

V

R

R

T

V

L

M

K

R

L

L

G

G

C

V

E

E

L

T

L

L

F

V

C

V

T

T

N

N

A
|
A

G
|
G

S

A

L

V

R

N

P

K

E

D

V

Y

G

T

P

P

G

G

S

D

L

L

V

M

A

I

L

I

S

S

D

D

H

H

I

L

N

N

T

L

M

S

P

G

G

S

T

N

P

P

M

L

V

I

G

G

P

K

N

N

D

L

E

N

R

E

F

F

G

G

E

T

R

R

F

F

F

P

S

D

L

M

A

S

N

N

A

A

Y

Y

D

D

A

K

D

D

Y

L

R

R

A

A

V

Q

L

V

Q

K

S

D

V

I

A

A

A

K

E

N

E

L

G

G

F

I

P

E

L

V

T

R

E

E

G

G

V

V

F

Y

V

A

S

M

Y

F

P

S

G

G

P

P

N

T

F
x
Y

E
|
E

T

T

A

P

A

A

E

E

I

V

R

R

M

M

M

A

Q

R

I

I

I

L

G

G

G

A

D

D

V

A

V

V

G

G

M
|
M

S

S

V

T

V

V

P

P

E

E

V

V

I

I

T

I

A

A

R

N

H

H

C

S

G

G

L

M

K

K

V

V

V

I

A

G

V

V

S

S

A

C

I

M

T

T

N
|
N

L

M

A

A

E

A

G

G

L

I

G

L

D

E

V

Q

K

P

L

L

S

N

H
|
H

A

E

Q

E

T
x
V

L

M

A

E

A

T

A

S

E

A

L

K

S

V

R

R

Q

K

N

T

F

F

I

I

N

E

L

L

I

M

C

T

G

N

F

I

L

I

R

K

K

E

L

I

binding 1,4-dideoxy-4-aza-1-(s)-(9-deazahypoxanthin-9-yl)-d-ribitol: Y82 (= Y89), A110 (= A117), A111 (= A118), G112 (= G119), Y189 (≠ F196), E190 (= E197), M208 (= M215), N232 (= N239), H244 (= H251), V247 (≠ T254)

4m1eB Crystal structure of purine nucleoside phosphorylase i from planctomyces limnophilus dsm 3776, nysgrc target 029364.
46% identity, 86% coverage: 26:262/277 of query aligns to 22:263/273 of 4m1eB

query
sites
4m1eB

P

P

R

A

V

V

A

G

F

M

I

I

L

L

G

G

S
x
T

G

G

L

L

G

G

A

G

L

L

A

A

E

E

Q

Q

I

I

E

E

D

Q

A

D

V

I

A

A

I

I

S

P

Y

Y

E

S

K

D

L

I

P

P

G

H

F

F

P

P

V

T

S

S

T

T

V

V

H

K

G

S

H
|
H

A

A

G

G

E

R

L

L

V

V

L

C

G

G

N

R

L

L

A

R

G

G

V

I

P

P

V

I

A

V

C

A

M

M

K

E

G

G

R

R

G

F

H
|
H

F

Y

Y
|
Y

E
|
E

G

G

R

Y

G

S

M

L

P

E

V

Q

M

V

T

T

D

F

A

P

I

V

R

R

T

V

L

M

K

K

L

A

L

M

G

G

C

V

E

K

L

T

L

L

F

L

C

V

T

T

N

N

A
|
A

G
|
G

S

G

L

I

R
x
N

P

P

E
x
Q

V

L

G

D

P

L

G

S

S

D

L

V

V

L

A

I

L

I

S

E

D

D

H

H

I

I

N

N

T

L

M

M

P

P

G

E

T

N

P

P

M

L

V

R

G

G

P

P

N

N

D

D

E

E

R

E

F

L

G

G

E

P

R

R

F

F

F

P

S

D

L

M

A

S

N

H

A

P

Y

Y

D

D

A

C

D

Q

Y

H

R

M

A

E

V

V

L

A

Q
x
R

S

Q

V

V

A

A

A

L

E

E

E

L

G

G

F

I

P
x
H

L

C

T
x
P

E

K

G

G

V

V

F

F

V

V

S

A

Y

V

P

S

G

G

P

P

N

N

F

L

E
|
E

T

T

A

R

A

A

E

E

I

Y

R

R

M

M

M

L

Q

K

I

L

I

M

G

G

G

A

D

D

V

V

V
|
V

G

G

M
|
M

S
|
S

V

T

V

V

P

P

E

E

V

V

I

L

T

V

A

A

R

V

H

H

C

A

G

G

L

L

K

R

V

V

V

L

A

G

V

F

S

S

A

V

I

V

T

T

N
x
D

L

L

A
x
C

-

L

-

P

E

D

G

A

L

L

G

E

D

P

V

V

K

E

L
|
L

S

N

H

K

A

I

Q

L

T

E

L

V

A

A

-

R

-

G

A

A

E

K

L

L

S

A

R

R

active site: T30 (≠ S34), H61 (= H65), H83 (= H87), Y85 (= Y89), E86 (= E90), A113 (= A117), M211 (= M215), S212 (= S216), D235 (≠ N239), C237 (≠ A241), L247 (= L249)
binding pyridine-2-carboxylic acid: N118 (≠ R122), Q120 (≠ E124), R169 (≠ Q173), H178 (≠ P182), P180 (≠ T184), P180 (≠ T184)
binding adenine: A114 (= A118), G115 (= G119), E193 (= E197), V209 (= V213), D235 (≠ N239)

A4Q998 Purine nucleoside phosphorylase; AgPNP; Inosine phosphorylase; Inosine-guanosine phosphorylase; EC 2.4.2.1 from Anopheles gambiae (African malaria mosquito) (see paper)
48% identity, 80% coverage: 21:241/277 of query aligns to 85:310/353 of A4Q998

query
sites
A4Q998

K

R

P

T

D

E

F

L

T

R

P

P

R

K

V

V

A

G

F

I

I

I

L

C

G

G

S
|
S

G

G

L

L

G

G

A

T

L

L

A

A

E

E

Q

Q

I

L

E

T

D

D

A

V

V

D

A

S

I

F

S

D

Y

Y

E

E

K

T

L

I

P

P

G

H

F

F

P

P

V

V

S

S

T

T

V

V

H

A

G

G

H
|
H

A

V

G

G

E

R

L

L

V

V

L

F

G

G

N

Y

L

L

A

A

G

G

V

V

P

P

V

V

A

M

C

C

M

M

K

Q

G

G

R
|
R

G
x
F

H
|
H

F

H

Y

Y

E

E

G

G

R

Y

G

P

M

L

P

A

V

K

M

C

T

A

D

M

A

P

I

V

R

R

T

V

L

M

K

H

L

L

L

I

G

G

C

C

E

T

L

H

L

L

F

I

C

A

T

T

N

N

A
|
A

A

A

G

G

S

G

L

A

R

N

P

P

E

K

V

Y

G

R

P

V

G

G

S

D

L

I

V

M

A

L

L

I

S

K

D

D

H

H

I

I

N

N

T

L

M

M

-

G

-

F

-

A

P

G

G

N

T

N

P

P

M

L

V

Q

G

G

P

P

N

N

D

D

E

E

R

R

F

F

G

G

E

P

R

R

F

F

S

G

L

M

A

A

N

N

A

T

Y

Y

D

D

A

P

D

K

Y

L

R

N

A

Q

V

Q

L

A

Q

K

S

V

V

I

A

A

A

R

E

Q

E

I

G

G

F

I

-

E

-

N

P

E

L

L

T

R

E

E

G

G

V

V

F

Y

V

T

S

C

Y

L

P

G

G

G

P

P

N

N

F

F

E

E

T

T

A

V

A

A

E

E

I

V

R

K

M

M

M

L

Q

S

I

M

I

L

G

G

G

V

D

D

V

A

V

I

G

G

M

M

S
|
S

V

T

V

V

P

H

E

E

V

I

I

I

T

T

A

A

R

R

H

H

C

C

G

G

L

M

K

T

V

C

V

F

A

A

V

F

S

S

A

L

I

I

T

T

N

N

L

M

A

C

S98 (= S34) binding
H129 (= H65) binding
RFH 149:151 (≠ RGH 85:87) binding
A181 (= A117) binding
S285 (= S216) binding

Sites not aligning to the query:

1:68 modified: Variant sequence, MSKFSYLQNGKASTNGVPHANGHHQQHQNGHSNGVARNGGTATDTLPVAYQQKAATSGPFHMPRTEHV -> M (in isoform 2)

2p4sA Structure of purine nucleoside phosphorylase from anopheles gambiae in complex with dadme-immh (see paper)
48% identity, 80% coverage: 21:241/277 of query aligns to 16:241/282 of 2p4sA

query
sites
2p4sA

K

R

P

T

D

E

F

L

T

R

P

P

R

K

V

V

A

G

F

I

I

I

L

C

G
|
G

S
|
S

G

G

L

L

G

G

A

T

L

L

A

A

E

E

Q

Q

I

L

E

T

D

D

A

V

V

D

A

S

I

F

S

D

Y

Y

E

E

K

T

L

I

P

P

G

H

F

F

P

P

V

V

S

S

T

T

V

V

H

A

G

G

H
|
H

A

V

G

G

E

R

L

L

V

V

L

F

G

G

N

Y

L

L

A

A

G

G

V

V

P

P

V

V

A

M

C

C

M

M

K

Q

G

G

R
|
R

G

F

H
|
H

F

H

Y
|
Y

E
|
E

G

G

R

Y

G

P

M

L

P

A

V

K

M

C

T

A

D

M

A

P

I

V

R

R

T

V

L

M

K

H

L

L

L

I

G

G

C

C

E

T

L

H

L

L

F

I

C

A

T

T

N
|
N

A
|
A

G
|
G

S

G

L

A

R

N

P

P

E

K

V

Y

G

R

P

V

G

G

S

D

L

I

V

M

A

L

L

I

S

K

D

D

H

H

I

I

N

N

T

L

M

M

-

G

-

F

-

A

P

G

G

N

T

N

P

P

M

L

V

Q

G

G

P

P

N

N

D

D

E

E

R

R

F

F

G

G

E

P

R

R

F

F

S

G

L

M

A

A

N

N

A

T

Y

Y

D

D

A

P

D

K

Y

L

R

N

A

Q

V

Q

L

A

Q

K

S

V

V

I

A

A

A

R

E

Q

E

I

G

G

F

I

-

E

-

N

P

E

L

L

T

R

E

E

G

G

V

V

F

Y

V

T

S

C

Y

L

P

G

G

G

P

P

N

N

F
|
F

E
|
E

T

T

A

V

A

A

E

E

I

V

R

K

M

M

M

L

Q

S

I

M

I

L

G

G

G

V

D

D

V

A

V
x
I

G
|
G

M
|
M

S
|
S

V

T

V

V

P

H

E

E

V

I

I

I

T

T

A

A

R

R

H

H

C

C

G

G

L

M

K

T

V

C

V

F

A

A

V

F

S

S

A

L

I

I

T

T

N
|
N

L

M

A
x
C

active site: S29 (= S34), H60 (= H65), H82 (= H87), Y84 (= Y89), E85 (= E90), A112 (= A117), M215 (= M215), S216 (= S216), N239 (= N239), C241 (≠ A241)
binding 7-[[(3R,4R)-3-(hydroxymethyl)-4-oxidanyl-pyrrolidin-1-ium-1-yl]methyl]-3,5-dihydropyrrolo[3,2-d]pyrimidin-4-one: Y84 (= Y89), A112 (= A117), A113 (= A118), G114 (= G119), F196 (= F196), E197 (= E197), I213 (≠ V213), G214 (= G214), M215 (= M215), N239 (= N239)
binding phosphate ion: G28 (= G33), S29 (= S34), R80 (= R85), H82 (= H87), N111 (= N116), S216 (= S216)

Sites not aligning to the query:

3lbaA The crystal structure of smu.1229 from streptococcus mutans ua159 bound to hypoxanthine
45% identity, 91% coverage: 26:276/277 of query aligns to 22:260/260 of 3lbaA

query
sites
3lbaA

P

P

R

E

V

F

A

G

F

L

I

I

L

L

G

G

S
|
S

G

G

L

L

G

G

A

E

L

L

A

A

E

E

Q

E

I

I

E

E

D

N

A

A

V

L

A

V

I

L

S

N

Y

Y

E

A

K

D

L

I

P

P

G

N

F

W

P

-

V

-

S

-

T

-

V

-

H

-

G

G

H

R

A

S

G

G

E

K

L

L

V

I

L

Y

G

G

N

E

L

L

A

A

G

G

V

R

P

K

V

V

A

L

C

A

M

L

K

Q

G

G

R

R

G

F

H
|
H

F

Y

Y
|
Y

E
|
E

G

G

R

N

G

S

M

M

P

E

V

L

M

V

T

T

D

F

A

P

I

I

R

R

T

I

L

M

K

K

L

A

L

L

G

G

C

C

E

Q

L

G

L

L

F

I

C

V

T

T

N

N

A
|
A

G
|
G

S

G

L

I

R

G

P

-

E

-

V

F

G

G

P

P

G

G

S

T

L

L

V

M

A

A

L

I

S

S

D

D

H

H

I

I

N

N

T

L

M

T

P

G

G

A

T

N

P

P

M

L

V

M

G

G

P

E

N

N

D

L

E

D

R

D

F

F

G

G

E

F

R

R

F

F

F

P

S

D

L

M

A

S

N

N

A

A

Y

Y

D

T

A

A

D

D

Y

Y

R

R

A

E

V

V

L

A

Q

H

S

Q

V

V

A

A

A

D

E

K

E

I

G

G

F

I

P

K

L

L

T

D

E

E

G

G

V

V

F

Y

V

I

S

G

Y

V

P

S

G

G

P

P

N

S

F
x
Y

E
|
E

T

T

A

P

A

A

E

E

I

I

R

R

M

A

M

F

Q

K

I

T

I

L

G

G

G

A

D

D

V

A

V
|
V

G
|
G

M
|
M

S
|
S

V

T

V

V

P

P

E

E

V

V

I

I

T

V

A

A

R

V

H

H

C

S

G

G

L

L

K

K

V

V

V

L

A

G

V

I

S

S

A

A

I

I

T

T

N
|
N

L

Y

A
|
A

E

A

G

G

L

F

G

-

D

-

V

-

K

-

L

Q

S

S

H

E

A

L

Q

N

T

V

L

V

A

A

A

V

A

T

E

Q

L

Q

S

I

R

K

Q

E

N

D

F

F

I

K

N

G

L

L

I

V

C

K

G

A

F

I

L

L

R

V

K

E

L

L

active site: S30 (= S34), H77 (= H87), Y79 (= Y89), E80 (= E90), A107 (= A117), M203 (= M215), S204 (= S216), N227 (= N239), A229 (= A241)
binding hypoxanthine: A107 (= A117), A108 (= A118), G109 (= G119), Y184 (≠ F196), E185 (= E197), V201 (= V213), G202 (= G214), N227 (= N239)

8swtA Structure of bacteroides fragilis pnp bound to transition state analog immucillin h and sulfate (see paper)
39% identity, 95% coverage: 13:275/277 of query aligns to 17:277/278 of 8swtA

query
sites
8swtA

S

T

A

A

D

A

I

F

I

L

R

K

G

G

Y

-

K

K

P

M

D

H

F

T

T

S

P

P

R

E

V

T

A

A

F

I

I

I

L

L

G

G

S

T

G

G

L

L

G

G

A

S

L

L

A

A

E

N

Q

E

I

I

E

T

D

E

A

K

V

Y

A

E

I

I

S

K

Y

Y

E

E

K

D

L

I

P

P

G

N

F

F

P

P

V

V

S

S

T

T

V

V

H

E

G

G

H

H

A

S

G

G

E

K

L

L

V

I

L

F

G

G

N

K

L

L

A

G

G

N

V

K

P

E

V

I

A

M

C

A

M

M

K

Q

G

G

R

R

G

F

H
|
H

F

Y

Y
|
Y

E

E

G

G

R

Y

G

S

M

M

P

K

V

E

M

V

T

T

D

F

A

P

I

V

R

R

T

V

L

M

K

R

L

E

L

L

G

G

C

I

E

K

L

T

L

L

F

F

C

V

T

S

N

N

A
|
A

A
x
S

G
|
G

S

G

L

T

R

N

P

P

E

E

V

F

G

E

P

I

G

G

S

D

L

L

V

M

A

I

L

I

S

T

D

D

H

H

I

I

N

N

T

Y

M

F

P

P

G

E

T

H

P

P

M

L

V

R

G

G

P

K

N

N

D

I

E

P

R

-

F

Y

G

G

E

P

R

R

F

F

F

P

S

D

L

M

A

S

N

E

A

A

Y

Y

D

D

A

K

D

E

Y

L

R

I

A

R

V

K

L

A

Q

D

S

A

V

I

A

A

E

E

E

K

G

G

F

I

P

K

L

V

T

Q

E

H

G

G

V

I

F

Y

V

I

S

G

Y

T

P

Q

G

G

P

P

N

T

F
|
F

E
|
E

T

T

A

P

A

A

E

E

I
x
Y

R

K

M

L

M

F

Q

H

I

I

I

L

G

G

G

A

D

D

V

A

V

V

G
|
G

M
|
M

S

S

V

T

V

V

P

P

E

E

V

V

I

I

T

V

A

A

R

N

H

H

C

C

G

G

L

I

K

K

V

V

V

F

A

G

V

I

S

S

A

V

I

V

T

T

N
x
D

L

L

A

G

E

V

G

E

L

G

G

K

D

I

V

V

K

E

L

V

S

S

H
|
H

A

E

Q

E

T

V

L

Q

A

K

A

A

E

D

L

A

S

A

R

Q

Q

P

N

K

F

M

I

T

N

T

L

I

I

M

C

R

G

E

F

L

L

I

R

N

K

R

binding 1,4-dideoxy-4-aza-1-(s)-(9-deazahypoxanthin-9-yl)-d-ribitol: H90 (= H87), Y92 (= Y89), A120 (= A117), S121 (≠ A118), G122 (= G119), F198 (= F196), E199 (= E197), Y204 (≠ I202), G216 (= G214), M217 (= M215), D241 (≠ N239), H253 (= H251)

3e0qC Crystal structure of schistosoma mansoni purine nucleoside phosphorylase complexed with a novel monocyclic inhibitor (see paper)
44% identity, 89% coverage: 24:269/277 of query aligns to 23:279/285 of 3e0qC

query
sites
3e0qC

F

I

T

V

P

P

R

E

V

I

A

G

F

I

I

I

L

C

G

G

S
|
S

G

G

L

L

G

G

A

K

L

L

A

A

E

D

Q

G

I

V

E

K

D

D

A

K

V

I

A

T

I

I

S

P

Y

Y

E

T

K

K

L

I

P

P

G

N

F

F

P

P

V

Q

S

T

T

S

V

V

H

V

G

G

H
|
H

A

S

G

G

E

N

L

L

V

I

L

F

G

G

N

T

L

L

A

S

G

G

V

R

P

K

V

V

A

V

C

V

M

M

K

Q

G

G

R

R

G

F

H
|
H

F

M

Y
|
Y

E
|
E

G

G

R

Y

G

S

M

N

P

D

V

T

M

V

T

A

D

L

A

P

I

I

R

R

T

V

L

M

K

K

L

L

G

G

C

V

E

K

L

I

L

L

F

M

C

V

T

S

N

N

A
|
A

A

A

G
|
G

S

G

L

L

R

N

P

R

E

S

V

L

G

K

P

L

G

G

S

D

L

F

V

V

A

I

L

L

S

K

D

D

H

H

I

I

N

-

T

Y

M

L

P

P

G

G

-

L

-

G

-

L

-

N

T

N

P

I

M

L

V

V

G

G

P

P

N

N

D

Q

E

E

R

A

F

F

G

G

E

T

R

R

F

F

F

P

S

A

L

L

A

S

N

N

A

A

Y

Y

D

D

A

R

D

D

Y

L

R

R

A

K

V

L

L

A

Q

V

S

Q

V

V

A

A

A

E

E

E

E

N

G

G

F

F

P

G

-

N

-

L

L

V

T

H

E

Q

G

G

V

V

F

Y

V

V

S

M

Y

N

P

G

G

G

P

P

N

C

F
x
Y

E
|
E

T

T

A

P

A

A

E

E

I

C

R

T

M

M

M

L

Q

L

I

N

I

M

G

G

G

C

D

D

V

V

G
|
G

M
|
M

S
|
S

V

T

V

I

P

P

E

E

V

V

I

V

T

I

A

A

R

R

H

H

C

C

G

G

L

I

K

Q

V

V

V

F

A

A

V

V

S

S

A

L

I

V

T

T

N
|
N

L

I

A
x
S

-

V

-

L

E

D

G

V

L

E

G

S

D

D

V

L

K

K

L

P

S

N

H
|
H

A

E

Q

E

T
x
V

L

L

A

A

A

T

-

G

-

A

-

Q

-

R

A

A

E

E

L

L

S

M

R

Q

Q

S

N

W

F

F

I

E

N

K

L

I

I

I

active site: S33 (= S34), H64 (= H65), H86 (= H87), Y88 (= Y89), E89 (= E90), A116 (= A117), M219 (= M215), S220 (= S216), N243 (= N239), S245 (≠ A241), H257 (= H251)
binding 6-amino-5-bromo-1,2,3,4-tetrahydropyrimidine-2,4-dione: A116 (= A117), G118 (= G119), Y200 (≠ F196), E201 (= E197), G218 (= G214), M219 (= M215), N243 (= N239), V260 (≠ T254)

1a9sA Bovine purine nucleoside phosphorylase complexed with inosine and sulfate (see paper)
45% identity, 88% coverage: 26:269/277 of query aligns to 25:278/282 of 1a9sA

query
sites
1a9sA

P

P

R

Q

V

V

A

A

F

V

I

I

L

C

G

G

S
|
S

G

G

L

L

G

G

A

G

L

L

A

V

E

N

Q

K

I

L

E

T

D

Q

A

A

V

Q

A

T

I

F

S

D

Y

Y

E

S

K

E

L

I

P

P

G

N

F

F

P

P

V

E

S

S

T

T

V

V

H

P

G

G

H
|
H

A

A

G

G

E

R

L

L

V

V

L

F

G

G

N

I

L

L

A

N

G

G

V

R

P

A

V

C

A

V

C

M

M

M

K

Q

G

G

R

R

G

F

H
|
H

F

M

Y
|
Y

E
|
E

G

G

R

Y

G

P

M

F

P

W

V

K

M

V

T

T

D

F

A

P

I

V

R

R

T

V

L

F

K

R

L

L

G

G

C

V

E

E

L

T

L

L

F

V

C

V

T

T

N

N

A
|
A

A

A

G
|
G

S

G

L

L

R

N

P

P

E

N

V

F

G

E

P

V

G

G

S

D

L

I

V

M

A

L

L

I

S

R

D

D

H

H

I

I

N

N

T

-

M

L

P

P

G

G

-

F

-

S

-

G

-

E

T

N

P

P

M

L

V

R

G

G

P

P

N

N

D

E

E

E

R

R

F

F

G

G

E

V

R

R

F

F

F

P

S

A

L

M

A

S

N

D

A

A

Y

Y

D

D

A

R

D

D

Y

M

R

R

A

Q

V

K

L

A

Q

H

S

S

V

T

A

W

A

K

E

Q

-

M

-

G

E

E

G

Q

F

R

P

E

L

L

T

Q

E

E

G

G

V

T

F

Y

V

V

S

M

Y

L

P

G

G

G

P

P

N

N

F
|
F

E
|
E

T

T

A

V

A

A

E

E

I

C

R

R

M

L

Q

R

I

N

I

L

G

G

G

A

D

D

V

A

V

V

G

G

M
|
M

S
|
S

V

T

V

V

P

P

E

E

V

V

I

I

T

V

A

A

R

R

H

H

C

C

G

G

L

L

K

R

V

V

V

F

A

G

V

F

S

S

A

L

I

I

T
|
T

N
|
N

-

K

-
x
V

L

I

A

M

E

D

G

T

L

E

G

S

D

Q

V

G

K

K

L

A

S

N

H
|
H

A

E

Q

E

T
x
V

L

L

A

E

A

A

A

G

E

K

L

Q

S

A

R

A

Q

Q

-

K

-

L

-

E

N

Q

F

F

I

V

N

S

L

L

I

L

active site: S33 (= S34), H64 (= H65), H86 (= H87), Y88 (= Y89), E89 (= E90), A116 (= A117), M219 (= M215), S220 (= S216), N243 (= N239), V245 (vs. gap), H257 (= H251)
binding inosine: Y88 (= Y89), A116 (= A117), G118 (= G119), F200 (= F196), E201 (= E197), M219 (= M215), T242 (= T238), N243 (= N239), H257 (= H251), V260 (≠ T254)

1a9qA Bovine purine nucleoside phosphorylase complexed with inosine (see paper)
45% identity, 88% coverage: 26:269/277 of query aligns to 25:278/282 of 1a9qA

query
sites
1a9qA

P

P

R

Q

V

V

A

A

F

V

I

I

L

C

G

G

S
|
S

G

G

L

L

G

G

A

G

L

L

A

V

E

N

Q

K

I

L

E

T

D

Q

A

A

V

Q

A

T

I

F

S

D

Y

Y

E

S

K

E

L

I

P

P

G

N

F

F

P

P

V

E

S

S

T

T

V

V

H

P

G

G

H
|
H

A

A

G

G

E

R

L

L

V

V

L

F

G

G

N

I

L

L

A

N

G

G

V

R

P

A

V

C

A

V

C

M

M

M

K

Q

G

G

R

R

G

F

H
|
H

F

M

Y
|
Y

E
|
E

G

G

R

Y

G

P

M

F

P

W

V

K

M

V

T

T

D

F

A

P

I

V

R

R

T

V

L

F

K

R

L

L

G

G

C

V

E

E

L

T

L

L

F

V

C

V

T

T

N

N

A
|
A

G
|
G

S

G

L

L

R

N

P

P

E

N

V

F

G

E

P

V

G

G

S

D

L

I

V

M

A

L

L

I

S

R

D

D

H

H

I

I

N

N

T

-

M

L

P

P

G

G

-

F

-

S

-

G

-

E

T

N

P

P

M

L

V

R

G

G

P

P

N

N

D

E

E

E

R

R

F

F

G

G

E

V

R

R

F

F

F

P

S

A

L

M

A

S

N

D

A

A

Y

Y

D

D

A

R

D

D

Y

M

R

R

A

Q

V

K

L

A

Q

H

S

S

V

T

A

W

A

K

E

Q

-

M

-

G

E

E

G

Q

F

R

P

E

L

L

T

Q

E

E

G

G

V

T

F

Y

V

V

S

M

Y

L

P

G

G

G

P

P

N

N

F
|
F

E
|
E

T

T

A

V

A

A

E

E

I

C

R

R

M

L

Q

R

I

N

I

L

G

G

G

A

D

D

V

A

V

V

G

G

M
|
M

S
|
S

V

T

V

V

P

P

E

E

V

V

I

I

T

V

A

A

R

R

H

H

C

C

G

G

L

L

K

R

V

V

V

F

A

G

V

F

S

S

A

L

I

I

T

T

N
|
N

-

K

-
x
V

L

I

A

M

E

D

G

T

L

E

G

S

D

Q

V

G

K

K

L

A

S

N

H
|
H

A

E

Q

E

T

V

L

L

A

E

A

A

A

G

E

K

L

Q

S

A

R

A

Q

Q

-

K

-

L

-

E

N

Q

F

F

I

V

N

S

L

L

I

L

active site: S33 (= S34), H64 (= H65), H86 (= H87), Y88 (= Y89), E89 (= E90), A116 (= A117), M219 (= M215), S220 (= S216), N243 (= N239), V245 (vs. gap), H257 (= H251)
binding hypoxanthine: A117 (= A118), G118 (= G119), F200 (= F196), E201 (= E197), M219 (= M215), N243 (= N239)

1a9tA Bovine purine nucleoside phosphorylase complexed with 9-deazainosine and phosphate (see paper)
45% identity, 88% coverage: 26:269/277 of query aligns to 25:278/284 of 1a9tA

query
sites
1a9tA

P

P

R

Q

V

V

A

A

F

V

I

I

L

C

G

G

S
|
S

G

G

L

L

G

G

A

G

L

L

A

V

E

N

Q

K

I

L

E

T

D

Q

A

A

V

Q

A

T

I

F

S

D

Y

Y

E

S

K

E

L

I

P

P

G

N

F

F

P

P

V

E

S

S

T

T

V

V

H

P

G

G

H
|
H

A

A

G

G

E

R

L

L

V

V

L

F

G

G

N

I

L

L

A

N

G

G

V

R

P

A

V

C

A

V

C

M

M

M

K

Q

G

G

R
|
R

G

F

H
|
H

F

M

Y
|
Y

E
|
E

G

G

R

Y

G

P

M

F

P

W

V

K

M

V

T

T

D

F

A

P

I

V

R

R

T

V

L

F

K

R

L

L

G

G

C

V

E

E

L

T

L

L

F

V

C

V

T

T

N
|
N

A
|
A

G
|
G

S

G

L

L

R

N

P

P

E

N

V

F

G

E

P

V

G

G

S

D

L

I

V

M

A

L

L

I

S

R

D

D

H

H

I

I

N

N

T

-

M

L

P

P

G

G

-

F

-

S

-

G

-

E

T

N

P

P

M

L

V

R

G

G

P

P

N

N

D

E

E

E

R

R

F

F

G

G

E

V

R

R

F

F

F

P

S

A

L

M

A

S

N

D

A

A

Y

Y

D

D

A

R

D

D

Y

M

R

R

A

Q

V

K

L

A

Q

H

S

S

V

T

A

W

A

K

E

Q

-

M

-

G

E

E

G

Q

F

R

P

E

L

L

T

Q

E

E

G

G

V

T

F

Y

V

V

S

M

Y

L

P

G

G

G

P

P

N

N

F
|
F

E
|
E

T

T

A

V

A

A

E

E

I

C

R

R

M

L

Q

R

I

N

I

L

G

G

G

A

D

D

V

A

V
|
V

G

G

M
|
M

S
|
S

V

T

V

V

P

P

E

E

V

V

I

I

T

V

A

A

R

R

H

H

C

C

G

G

L

L

K

R

V

V

V

F

A

G

V

F

S

S

A

L

I

I

T

T

N
|
N

-

K

-
x
V

L

I

A

M

E

D

G

T

L

E

G

S

D

Q

V

G

K

K

L

A

S

N

H
|
H

A

E

Q

E

T
x
V

L

L

A

E

A

A

A

G

E

K

L

Q

S

A

R

A

Q

Q

-

K

-

L

-

E

N

Q

F

F

I

V

N

S

L

L

I

L

active site: S33 (= S34), H64 (= H65), H86 (= H87), Y88 (= Y89), E89 (= E90), A116 (= A117), M219 (= M215), S220 (= S216), N243 (= N239), V245 (vs. gap), H257 (= H251)
binding hypoxanthine: A116 (= A117), A117 (= A118), G118 (= G119), F200 (= F196), E201 (= E197), V217 (= V213), N243 (= N239)
binding 1-O-phosphono-alpha-D-ribofuranose: S33 (= S34), R84 (= R85), H86 (= H87), Y88 (= Y89), N115 (= N116), A116 (= A117), F200 (= F196), M219 (= M215), S220 (= S216), V260 (≠ T254)

1a9pA Bovine purine nucleoside phosphorylase complexed with 9-deazainosine and phosphate (see paper)
45% identity, 88% coverage: 26:269/277 of query aligns to 25:278/289 of 1a9pA

query
sites
1a9pA

P

P

R

Q

V

V

A

A

F

V

I

I

L

C

G
|
G

S
|
S

G

G

L

L

G

G

A

G

L

L

A

V

E

N

Q

K

I

L

E

T

D

Q

A

A

V

Q

A

T

I

F

S

D

Y

Y

E

S

K

E

L

I

P

P

G

N

F

F

P

P

V

E

S

S

T

T

V

V

H

P

G

G

H
|
H

A

A

G

G

E

R

L

L

V

V

L

F

G

G

N

I

L

L

A

N

G

G

V

R

P

A

V

C

A

V

C

M

M

M

K

Q

G

G

R
|
R

G

F

H
|
H

F

M

Y
|
Y

E
|
E

G

G

R

Y

G

P

M

F

P

W

V

K

M

V

T

T

D

F

A

P

I

V

R

R

T

V

L

F

K

R

L

L

G

G

C

V

E

E

L

T

L

L

F

V

C

V

T

T

N
|
N

A
|
A

A

A

G
|
G

S

G

L

L

R

N

P

P

E

N

V

F

G

E

P

V

G

G

S

D

L

I

V

M

A

L

L

I

S

R

D

D

H

H

I

I

N

N

T

-

M

L

P

P

G

G

-

F

-

S

-

G

-

E

T

N

P

P

M

L

V

R

G

G

P

P

N

N

D

E

E

E

R

R

F

F

G

G

E

V

R

R

F

F

F

P

S

A

L

M

A

S

N

D

A

A

Y

Y

D

D

A

R

D

D

Y

M

R

R

A

Q

V

K

L

A

Q

H

S

S

V

T

A

W

A

K

E

Q

-

M

-

G

E

E

G

Q

F

R

P

E

L

L

T

Q

E

E

G

G

V

T

F

Y

V

V

S

M

Y

L

P

G

G

G

P

P

N

N

F
|
F

E
|
E

T

T

A

V

A

A

E

E

I

C

R

R

M

L

Q

R

I

N

I

L

G

G

G

A

D

D

V

A

V

V

G

G

M
|
M

S
|
S

V

T

V

V

P

P

E

E

V

V

I

I

T

V

A

A

R

R

H

H

C

C

G

G

L

L

K

R

V

V

V

F

A

G

V

F

S

S

A

L

I

I

T

T

N
|
N

-

K

-
x
V

L

I

A

M

E

D

G

T

L

E

G

S

D

Q

V

G

K

K

L

A

S

N

H
|
H

A

E

Q
x
E

T

V

L

L

A

E

A

A

A

G

E

K

L

Q

S

A

R

A

Q

Q

-

K

-

L

-

E

N

Q

F

F

I

V

N

S

L

L

I

L

active site: S33 (= S34), H64 (= H65), H86 (= H87), Y88 (= Y89), E89 (= E90), A116 (= A117), M219 (= M215), S220 (= S216), N243 (= N239), V245 (vs. gap), H257 (= H251)
binding 9-deazainosine: H86 (= H87), Y88 (= Y89), A116 (= A117), G118 (= G119), F200 (= F196), E201 (= E197), M219 (= M215), N243 (= N239), H257 (= H251), E259 (≠ Q253)
binding phosphate ion: G32 (= G33), S33 (= S34), H64 (= H65), R84 (= R85), H86 (= H87), N115 (= N116), S220 (= S216)

1a9oA Bovine purine nucleoside phosphorylase complexed with phosphate (see paper)
45% identity, 88% coverage: 26:269/277 of query aligns to 25:278/289 of 1a9oA

query
sites
1a9oA

P

P

R

Q

V

V

A

A

F

V

I

I

L

C

G
|
G

S
|
S

G

G

L

L

G

G

A

G

L

L

A

V

E

N

Q

K

I

L

E

T

D

Q

A

A

V

Q

A

T

I

F

S

D

Y

Y

E

S

K

E

L

I

P

P

G

N

F

F

P

P

V

E

S

S

T

T

V

V

H

P

G

G

H
|
H

A

A

G

G

E

R

L

L

V

V

L

F

G

G

N

I

L

L

A

N

G

G

V

R

P

A

V

C

A

V

C

M

M

M

K

Q

G

G

R
|
R

G

F

H
|
H

F

M

Y
|
Y

E
|
E

G

G

R

Y

G

P

M

F

P

W

V

K

M

V

T

T

D

F

A

P

I

V

R

R

T

V

L

F

K

R

L

L

G

G

C

V

E

E

L

T

L

L

F

V

C

V

T

T

N
|
N

A
|
A

A

A

G

G

S

G

L

L

R

N

P

P

E

N

V

F

G

E

P

V

G

G

S

D

L

I

V

M

A

L

L

I

S

R

D

D

H

H

I

I

N

N

T

-

M

L

P

P

G

G

-

F

-

S

-

G

-

E

T

N

P

P

M

L

V

R

G

G

P

P

N

N

D

E

E

E

R

R

F

F

G

G

E

V

R

R

F

F

F

P

S

A

L

M

A

S

N

D

A

A

Y

Y

D

D

A

R

D

D

Y

M

R

R

A

Q

V

K

L

A

Q

H

S

S

V

T

A

W

A

K

E

Q

-

M

-

G

E

E

G

Q

F

R

P

E

L

L

T

Q

E

E

G

G

V

T

F

Y

V

V

S

M

Y

L

P

G

G

G

P

P

N

N

F

F

E

E

T

T

A

V

A

A

E

E

I

C

R

R

M

L

Q

R

I

N

I

L

G

G

G

A

D

D

V

A

V

V

G

G

M
|
M

S
|
S

V

T

V

V

P

P

E

E

V

V

I

I

T

V

A

A

R

R

H

H

C

C

G

G

L

L

K

R

V

V

V

F

A

G

V

F

S

S

A

L

I

I

T

T

N
|
N

-

K

-
x
V

L

I

A

M

E

D

G

T

L

E

G

S

D

Q

V

G

K

K

L

A

S

N

H
|
H

A

E

Q

E

T

V

L

L

A

E

A

A

A

G

E

K

L

Q

S

A

R

A

Q

Q

-

K

-

L

-

E

N

Q

F

F

I

V

N

S

L

L

I

L

active site: S33 (= S34), H64 (= H65), H86 (= H87), Y88 (= Y89), E89 (= E90), A116 (= A117), M219 (= M215), S220 (= S216), N243 (= N239), V245 (vs. gap), H257 (= H251)
binding phosphate ion: G32 (= G33), R84 (= R85), N115 (= N116), S220 (= S216)

1b8oA Purine nucleoside phosphorylase (see paper)
45% identity, 88% coverage: 26:269/277 of query aligns to 23:276/280 of 1b8oA

query
sites
1b8oA

P

P

R

Q

V

V

A

A

F

V

I

I

L

C

G
|
G

S
|
S

G

G

L

L

G

G

A

G

L

L

A

V

E

N

Q

K

I

L

E

T

D

Q

A

A

V

Q

A

T

I

F

S

D

Y

Y

E

S

K

E

L

I

P

P

G

N

F

F

P

P

V

E

S

S

T

T

V

V

H

P

G

G

H
|
H

A

A

G

G

E

R

L

L

V

V

L

F

G

G

N

I

L

L

A

N

G

G

V

R

P

A

V

C

A

V

C

M

M

M

K

Q

G

G

R
|
R

G

F

H
|
H

F

M

Y
|
Y

E
|
E

G

G

R

Y

G

P

M

F

P

W

V

K

M

V

T

T

D

F

A

P

I

V

R

R

T

V

L

F

K

R

L

L

G

G

C

V

E

E

L

T

L

L

F

V

C

V

T

T

N
|
N

A
|
A

A

A

G
|
G

S

G

L

L

R

N

P

P

E

N

V

F

G

E

P

V

G

G

S

D

L

I

V

M

A

L

L

I

S

R

D

D

H

H

I

I

N

N

T

-

M

L

P

P

G

G

-

F

-

S

-

G

-

E

T

N

P

P

M

L

V

R

G

G

P

P

N

N

D

E

E

E

R

R

F

F

G

G

E

V

R

R

F

F

F

P

S

A

L

M

A

S

N

D

A

A

Y

Y

D

D

A

R

D

D

Y

M

R

R

A

Q

V

K

L

A

Q

H

S

S

V

T

A

W

A

K

E

Q

-

M

-

G

E

E

G

Q

F

R

P

E

L

L

T

Q

E

E

G

G

V

T

F

Y

V

V

S

M

Y

L

P

G

G

G

P

P

N

N

F
|
F

E
|
E

T

T

A

V

A

A

E

E

I

C

R

R

M

L

Q

R

I

N

I

L

G

G

G

A

D

D

V

A

V

V

G

G

M
|
M

S
|
S

V

T

V

V

P

P

E

E

V

V

I

I

T

V

A

A

R

R

H

H

C

C

G

G

L

L

K

R

V

V

V

F

A

G

V

F

S

S

A

L

I

I

T

T

N
|
N

-

K

-
x
V

L

I

A

M

E

D

G

Y

L

E

G

S

D

Q

V

G

K

K

L

A

S

N

H
|
H

A

E

Q

E

T
x
V

L

L

A

E

A

A

A

G

E

K

L

Q

S

A

R

A

Q

Q

-

K

-

L

-

E

N

Q

F

F

I

V

N

S

L

L

I

L

active site: S31 (= S34), H62 (= H65), H84 (= H87), Y86 (= Y89), E87 (= E90), A114 (= A117), M217 (= M215), S218 (= S216), N241 (= N239), V243 (vs. gap), H255 (= H251)
binding 1,4-dideoxy-4-aza-1-(s)-(9-deazahypoxanthin-9-yl)-d-ribitol: H84 (= H87), Y86 (= Y89), A114 (= A117), G116 (= G119), F198 (= F196), E199 (= E197), M217 (= M215), N241 (= N239), H255 (= H251), V258 (≠ T254)
binding phosphate ion: G30 (= G33), S31 (= S34), R82 (= R85), H84 (= H87), N113 (= N116), S218 (= S216)

1b8nA Purine nucleoside phosphorylase (see paper)
45% identity, 88% coverage: 26:269/277 of query aligns to 24:277/278 of 1b8nA

query
sites
1b8nA

P

P

R

Q

V

V

A

A

F

V

I

I

L

C

G

G

S
|
S

G

G

L

L

G

G

A

G

L

L

A

V

E

N

Q

K

I

L

E

T

D

Q

A

A

V

Q

A

T

I

F

S

D

Y

Y

E

S

K

E

L

I

P

P

G

N

F

F

P

P

V

E

S

S

T

T

V

V

H

P

G

G

H
|
H

A

A

G

G

E

R

L

L

V

V

L

F

G

G

N

I

L

L

A

N

G

G

V

R

P

A

V

C

A

V

C

M

M

M

K

Q

G

G

R

R

G

F

H
|
H

F

M

Y
|
Y

E
|
E

G

G

R

Y

G

P

M

F

P

W

V

K

M

V

T

T

D

F

A

P

I

V

R

R

T

V

L

F

K

R

L

L

G

G

C

V

E

E

L

T

L

L

F

V

C

V

T

T

N

N

A
|
A

A

A

G
|
G

S

G

L

L

R

N

P

P

E

N

V

F

G

E

P

V

G

G

S

D

L

I

V

M

A

L

L

I

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D

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active site: S32 (= S34), H63 (= H65), H85 (= H87), Y87 (= Y89), E88 (= E90), A115 (= A117), M218 (= M215), S219 (= S216), N242 (= N239), V244 (vs. gap), H256 (= H251)
binding 1,4-dideoxy-1,4-imino-1-(s)-(9-deazaguanin-9-yl)-d-ribitol: H85 (= H87), Y87 (= Y89), A115 (= A117), G117 (= G119), F199 (= F196), E200 (= E197), V216 (= V213), G217 (= G214), M218 (= M215), N242 (= N239), H256 (= H251), V259 (≠ T254)

P55859 Purine nucleoside phosphorylase; PNP; Inosine phosphorylase; Inosine-guanosine phosphorylase; EC 2.4.2.1 from Bos taurus (Bovine) (see paper)
45% identity, 88% coverage: 26:269/277 of query aligns to 25:278/289 of P55859

query
sites
P55859

P

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S33 (= S34) binding
H64 (= H65) binding
RFH 84:86 (≠ RGH 85:87) binding
Y88 (= Y89) binding
A116 (= A117) binding
E201 (= E197) binding
M219 (= M215) binding
S220 (= S216) binding
N243 (= N239) binding
H257 (= H251) binding

Query Sequence

>BWI76_RS20605 FitnessBrowser__Koxy:BWI76_RS20605
MSDTLFSQNPWYSADIIRGYKPDFTPRVAFILGSGLGALAEQIEDAVAISYEKLPGFPVS
TVHGHAGELVLGNLAGVPVACMKGRGHFYEGRGMPVMTDAIRTLKLLGCELLFCTNAAGS
LRPEVGPGSLVALSDHINTMPGTPMVGPNDERFGERFFSLANAYDADYRAVLQSVAAEEG
FPLTEGVFVSYPGPNFETAAEIRMMQIIGGDVVGMSVVPEVITARHCGLKVVAVSAITNL
AEGLGDVKLSHAQTLAAAELSRQNFINLICGFLRKLA

Or try a new SitesBLAST search

SitesBLAST's Database

SitesBLAST's database includes (1) SwissProt entries with experimentally-supported functional features; and (2) protein structures with bound ligands, from the BioLip database.

by Morgan Price, Arkin group
Lawrence Berkeley National Laboratory