SitesBLAST

SitesBLAST – Find functional sites

SitesBLAST

Comparing BWI76_RS22410 FitnessBrowser__Koxy:BWI76_RS22410 to proteins with known functional sites using BLASTp with E ≤ 0.001.

Or try Sites on a Tree, PaperBLAST, Conserved Domains, or compare to all protein structures

Found 3 hits to proteins with known functional sites (download)

P69791 PTS system N,N'-diacetylchitobiose-specific EIIA component; EIIA-Chb; EIII-Chb; IIIcel; N,N'-diacetylchitobiose-specific phosphotransferase enzyme IIA component from Escherichia coli (strain K12) (see paper)
64% identity, 92% coverage: 2:97/104 of query aligns to 16:111/116 of P69791

query
sites
P69791

E

E

L

L

E

E

Q

V

V

V

M

M

G

G

I

L

I

I

N

N

A

S

G

G

Q

Q

S

A

R

R

S

S

L

L

C

A

Y

Y

E

A

A

A

L

L

S

K

C

Q

A

A

K

K

A

Q

G

G

D

D

F

F

T

A

A

A

D

K

E

A

K

M

M

M

K

D

E

Q

A

S

A

R

H

M

F

A

A

L

R

N

E

E

A

A

H

H

R

L

V

V

Q

Q

T

T

Q

K

L

L

I

I

E

E

A

G

D

D

E

A

G

G

E

E

G

G

K

K

T

M

K

K

M

V

T

S

L

L

V

V

M

L

V

V

H

H

A

A

Q

Q

D
|
D

H

H

L

L

M

M

T

T

S

S

I

M

L

L

A

A

K

R

E

E

L

L

I

I

G

T

E

E

L

L

I

I

A

E

I

L

Y

H

D92 (= D78) mutation to L: Eliminates the need for a metal cation at the homotrimer interface by substituting hydrophobic methyl-methyl interactions in place of the metal cation.

P0A0D6 PTS system lactose-specific EIIA component; EIIA-Lac; EIII-Lac; Lactose-specific phosphotransferase enzyme IIA component from Staphylococcus aureus (see 2 papers)
44% identity, 91% coverage: 4:98/104 of query aligns to 5:101/103 of P0A0D6

query
sites
P0A0D6

E

E

E

V

Q

Q

V

L

M

L

G

G

-

F

-

E

I

I

I

V

I

A

N

F

A

A

G

G

Q

D

S

A

R

R

S

S

L

K

C

F

Y

L

E

E

A

A

L

L

S

T

C

A

A

A

K

Q

A

A

G

G

D

D

F

F

T

A

A

K

A

A

D

D

E

A

K

L

M

I

K

E

E

E

A

G

A

N

H

N

F

C

A

I

R

A

E

E

A

A

H

H

R

R

V

A

Q

Q

T

T

Q

S

L

L

I

L

E

-

A

A

D

K

E

E

G

A

E

Q

G

G

K

D

-

D

T

I

K

A

M

Y

T

S

L

V

V

T

M

M

V

M

H
|
H

A

G

Q

Q

D

D

H
|
H

L

L

M

M

T

T

S

T

I

I

L

L

A

L

K

K

E

D

L

L

I

M

G

K

E

H

L

L

I

L

A

E

I

F

Y

Y

R

K

H78 (= H75) active site, Tele-phosphohistidine intermediate; mutation to S: Abolished activity.
H82 (= H79) mutation to S: Reduced activity.

P23532 PTS system lactose-specific EIIA component; EIIA-Lac; EIII-Lac; Lactose-specific phosphotransferase enzyme IIA component from Lactococcus lactis subsp. lactis (Streptococcus lactis) (see 2 papers)
40% identity, 86% coverage: 10:98/104 of query aligns to 13:101/105 of P23532

query
sites
P23532

I

I

I

V

I

A

N

Y

A

A

G
|
G

Q

D

S

A

R

R

S

S

L

K

C

L

Y

L

E

E

A

A

L

L

S

K

C

A

A

A

K

E

A

N

G

G

D

D

F

F

T

A

A

K

A

A

D

D

E

S

K

L

M

V

K

V

E

E

A

A

A

G

H

S

F

C

A

I

R

A

E

E

A

A

H

H

R

S

V

S

Q

Q

T

T

Q

G

L

M

I

L

E

A

A

R

D

E

E

A

G

S

E

G

G

E

K

E

T

L

K

P

M

Y

T

S

L

V

V

T

M

M

V

M

H

H

A

G

Q

Q

D
|
D

H

H

L

L

M

M

T

T

S

T

I

I

L

L

A

L

K

K

E

D

L

V

I

I

G

H

E

H

L

L

I

I

A

E

I

L

Y

Y

R

K

G18 (= G15) mutation to E: No activity.
D81 (= D78) binding

Query Sequence

>BWI76_RS22410 FitnessBrowser__Koxy:BWI76_RS22410
MELEEQVMGIIINAGQSRSLCYEALSCAKAGDFTAADEKMKEAAHFAREAHRVQTQLIEA
DEGEGKTKMTLVMVHAQDHLMTSILAKELIGELIAIYRSRPLHA

Or try a new SitesBLAST search

SitesBLAST's Database

SitesBLAST's database includes (1) SwissProt entries with experimentally-supported functional features; and (2) protein structures with bound ligands, from the BioLip database.

by Morgan Price, Arkin group
Lawrence Berkeley National Laboratory