SitesBLAST

SitesBLAST – Find functional sites

SitesBLAST

Comparing BWI76_RS23705 FitnessBrowser__Koxy:BWI76_RS23705 to proteins with known functional sites using BLASTp with E ≤ 0.001.

Or try Sites on a Tree, PaperBLAST, Conserved Domains, or compare to all protein structures

Found 20 (the maximum) hits to proteins with known functional sites (download)

7tzpG Crystal structure of putataive short-chain dehydrogenase/reductase (fabg) from klebsiella pneumoniae subsp. Pneumoniae ntuh-k2044 in complex with nadh (see paper)
97% identity, 100% coverage: 1:244/244 of query aligns to 4:247/247 of 7tzpG

query
sites
7tzpG

M

M

K

K

L

L

A

A

S

S

K

K

T

T

A

A

I

I

V

V

T

T

G
|
G

A

A

R
|
R

G
|
G

I
|
I

G

G

F

F

G

G

I

I

A

A

Q

Q

V

V

L

L

A

A

R

R

E

E

G

G

A

A

R

R

V

V

I

I

A

A

D
|
D

R
|
R

D

D

A

A

H

H

G

G

E

E

A

A

S

S

L

L

R

R

E

E

S

S

G

G

A

A

Q

Q

A

A

L

L

F

F

I

I

S

S

C
|
C

N

N

I
|
I

G

A

D

E

K

K

A

T

Q

Q

V

V

E

E

A

A

L

L

F

F

S

S

Q

Q

A

A

E

E

A

A

F

F

G

G

A

P

V

V

D

D

I

I

V

L

V

V

N

N

N
|
N

A

A

G
|
G

I
|
I

N

N

R

R

D

D

A

A

M

M

L

L

H

H

K

K

L

L

S

T

E

E

A

A

D

D

W

W

D

D

T

T

V

V

I

I

D

D

V
|
V

N

N

L

L

K

K

G

G

T

T

F

F

L

L

C

C

M

M

Q

Q

A

A

I

I

R

R

M

M

R

R

E

E

R

R

G

G

A

A

G

G

R

R

I

I

N

N

I

I

A

A

S

S

A

A

S

S

W

W

L

L

G

G

N

N

V

V

G

G

Q

Q

T

T

N

N

Y
|
Y

S

S

A

A

S

S

K
|
K

A

A

G

G

V

V

G

G

M

M

T

T

K

K

T

T

A

A

C

C

R

R

E

E

L

L

A

A

K

K

G

G

V

V

T

T

V

V

N

N

A

A

I

I

C

C

P

P

G

G

F

F

I
|
I

D

D

T
|
T

D

D

M

M

T
|
T

R

R

G

G

V

V

P

P

E

E

N

N

V

V

W

W

Q

Q

I

I

M

M

I

V

N

S

K

K

I

I

P

P

A

A

G

G

Y

Y

A

A

G

G

E

E

A

A

K

K

D

D

V

V

G

G

E

E

C

C

V

V

A

A

F

F

L

L

A

A

S

S

D

D

G

G

A

A

R

R

Y

Y

I

I

N

N

G

G

E

E

V

V

I

I

N

N

V

V

G

G

M

M

V

V

L

L

binding 1,4-dihydronicotinamide adenine dinucleotide: G15 (= G12), R18 (= R15), G19 (= G16), I20 (= I17), D39 (= D36), R40 (= R37), C63 (= C60), I65 (= I62), N91 (= N88), G93 (= G90), I94 (= I91), V114 (= V111), Y155 (= Y152), K159 (= K156), I188 (= I185), T190 (= T187), T193 (= T190)

4jroC Crystal structure of 3-oxoacyl-[acyl-carrier protein]reductase (fabg) from listeria monocytogenes in complex with NADP+
48% identity, 100% coverage: 1:244/244 of query aligns to 1:247/247 of 4jroC

query
sites
4jroC

M

M

K

T

L

L

A

Q

S

G

K

K

T

V

A

A

I

V

V

V

T

T

G
|
G

A

G

A
x
S

R
|
R

G
|
G

I
|
I

G

G

F

R

G

D

I

I

A

A

Q

I

V

N

L

L

A

A

R

K

E

E

G

G

A

A

R

N

V

I

I

F

A

-

D
x
N

R
x
Y

D
x
N

A
x
G

H
x
S

G

P

E

E

A

A

A

E

S

T

L

A

R

K

-

L

-

V

-

A

E

E

S

H

G

G

A

V

Q

E

A

V

L

E

F

A

I

M

S

K

C

A

N
|
N

I
x
V

G

A

D

I

K

A

A

E

Q

D

V

V

E

D

A

A

L

F

F

F

S

K

Q

Q

A

A

E

I

E

E

A

R

F

F

G

G

A

R

V

V

D

D

I

I

V

L

V

V

N

N

N
|
N

A
|
A

G

G

I
|
I

N

T

R

R

D

D

A

N

M

L

L

L

H

M

K

R

L

M

S

K

E

E

A

D

D

E

W

W

D

D

T

D

V

V

I

I

D

N

V
x
I

N

N

L

L

K

K

G

G

T

T

F

F

L

L

C

C

M

T

Q

K

Q

A

A

V

A

S

I

R

R

T

M

M

R

M

E

K

R

Q

G

R

A

A

G

G

R

K

I

I

N

N

I

M

A

A

S
|
S

A

V

S

V

W

G

L

L

-

I

G

G

N

N

V

A

G

G

Q
|
Q

T

A

N

N

Y
|
Y

S

V

A

A

S

S

K
|
K

A

A

G

G

V

V

V

I

G

G

M

L

T

T

K

K

T

T

A

T

C

A

R

R

E

E

L

L

A

A

K

P

K

R

G

G

V

I

T

N

V

V

N

N

A

A

I

V

C

A

P
|
P

G

G

F

F

I
|
I

D

T

T
|
T

D

D

M

M

T

T

R

D

G

K

V

L

P

D

E

E

N

K

V

T

W

K

Q

E

I

A

M

M

I

L

N

A

K

Q

I

I

P

P

A

L

G

G

Y

A

A

Y

G

G

E

T

A

T

K

E

D

D

V

I

G

A

E

N

C

A

V

V

A

L

F

F

L

L

A

A

S

S

D

D

G

A

A

S

R

K

Y

Y

I

I

N

T

G

G

E

Q

V

T

I

L

N

S

V

V

G

D

G

G

M

M

V

V

L

M

active site: G16 (= G16), S142 (= S140), Q152 (= Q149), Y155 (= Y152), K159 (= K156)
binding nadp nicotinamide-adenine-dinucleotide phosphate: G12 (= G12), S14 (≠ A14), R15 (= R15), G16 (= G16), I17 (= I17), N35 (≠ D36), Y36 (≠ R37), N37 (≠ D38), G38 (≠ A39), S39 (≠ H40), N63 (= N61), V64 (≠ I62), N90 (= N88), A91 (= A89), I93 (= I91), I113 (≠ V111), S142 (= S140), Y155 (= Y152), K159 (= K156), P185 (= P182), I188 (= I185), T190 (= T187)

3osuA Crystal structure of the 3-oxoacyl-acyl carrier protein reductase, fabg, from staphylococcus aureus
45% identity, 100% coverage: 1:244/244 of query aligns to 1:246/246 of 3osuA

query
sites
3osuA

M

M

K

K

L

M

A

-

S

T

K

K

T

S

A

A

I

L

V

V

T

T

G

G

A

A

A

S

R

R

G
|
G

I

I

G

G

F

R

G

S

I

I

A

A

Q

L

V

Q

L

L

A

A

R

E

E

E

G

G

A

Y

R

N

V

V

I

A

I

V

-

N

-

Y

A

A

D

G

R

S

D

K

A

E

H

K

G

A

E

E

A

A

A

V

A

E

S

E

L

I

R

K

E

A

S

K

G

G

A

V

Q

D

A

S

L

F

F

A

I

I

S

Q

C

A

N

N

I

V

G

A

D

D

K

A

A

D

Q

E

V

V

E

K

A

A

L

M

F

I

S

K

Q

E

A

V

E

V

E

S

A

Q

F

F

G

G

A

S

V

L

D

D

I

V

V

L

V

V

N

N

N
|
N

A

A

G

G

I

I

N

T

R

R

D

D

A

N

M

L

L

L

H

M

K

R

L

M

S

K

E

E

A

Q

D

E

W

W

D

D

T

D

V

V

I

I

D

D

V

T

N

N

L

L

K

K

G

G

T

V

F

F

L

N

C

C

M

I

Q

Q

Q

K

A

A

A

T

I

P

R

Q

M

M

R

L

E

R

R

Q

G

R

A

S

G

G

R

A

I

I

N

N

I

L

A

S

S
|
S

A

V

-

V

S

G

W

A

L

V

G

G

N

N

V

P

G

G

Q
|
Q

T

A

N

N

Y
|
Y

S

V

A

A

S

T

K
|
K

A

A

G

G

V

V

V

I

G

G

M

L

T

T

K

K

T

S

A

A

C

A

R

R

E

E

L

L

A

A

K

S

K

R

G

G

V

I

T

T

V

V

N

N

A

A

I

V

C

A

P

P

G

G

F

F

I

I

D

V

T

S

D

D

M

M

T

T

R

D

G

A

V

L

P

S

E

D

N

E

V

L

W

K

Q

E

I

Q

M

M

I

L

N

T

K

Q

I

I

P

P

A

L

G

A

Y

R

A

F

G

G

E

Q

A

D

K

T

D

D

V

I

G

A

E

N

C

T

V

V

A

A

F

F

L

L

A

A

S

S

D

D

G

K

A

A

R

K

Y

Y

I

I

N

T

G

G

E

Q

V

T

I

I

N

H

V

V

G

N

G

G

M

M

V

Y

L

M

active site: G15 (= G16), S141 (= S140), Q151 (= Q149), Y154 (= Y152), K158 (= K156)
binding magnesium ion: N89 (= N88), K158 (= K156)

4nbuB Crystal structure of fabg from bacillus sp (see paper)
46% identity, 100% coverage: 2:244/244 of query aligns to 4:244/244 of 4nbuB

query
sites
4nbuB

K

R

L

L

A

Q

S

D

K

K

T

V

A

A

I

I

V

I

T

T

G
|
G

A

A

R
x
N

G
|
G

I
|
I

G

G

F

L

G

E

I

A

A

A

Q

R

V

V

L

F

A

M

R

K

E

E

G

G

A

A

R

K

V

V

I

V

I

I

A

A

D
|
D

R
x
F

D

N

A

-

H

-

G

-

E

E

A

A

A

G

A

K

S

E

L

A

R

V

E

E

S

A

G

N

A

P

Q

G

A

V

L

V

F

F

I

I

S

R

C
x
V

N
x
D

I
x
V

G

S

D

D

K

R

A

E

Q

S

V

V

E

H

A

R

L

L

F

V

S

E

Q

N

A

V

E

A

E

E

A

R

F

F

G

G

A

K

V

I

D

D

I

I

V

L

V

I

N

N

N
|
N

A
|
A

G
|
G

I
|
I

N

T

R

R

D
|
D

A

S

M

M

L

L

H

S

K
|
K

L

M

S

T

E

V

A

D

D

Q

W

F

D

Q

T

Q

V

V

I

I

D

N

V

V

N
|
N

L

L

K

T

G

G

T

V

F

F

L

H

C

C

M

T

Q

Q

Q

A

A

V

A

L

I

P

R

Y

M

M

R

A

E

E

R

Q

G

G

A

K

G

G

R

K

I

I

N

N

I
x
T

A

S

S
|
S

A

V

S

T

-

G

W

T

L

Y

G

G

N
|
N

V
|
V

G

G

Q
|
Q

T

T

N

N

Y
|
Y

S

A

A

A

S

A

K
|
K

A

A

G

G

V

V

V

I

G

G

M

M

T

T

K

K

T

T

A

W

C

A

R

K

E

E

L

L

A

A

K

R

K

K

G

G

V

I

T

N

V

V

N

N

A

A

I

V

C

A

P
|
P

G

G

F
|
F

I
x
T

D

E

T
|
T

D

A

M
|
M

T

V

R

A

G

E

V

V

P

P

E

E

N

K

V

V

W

I

Q

E

I
x
K

M

M

I

K

N

A

K

Q

I

V

P

P

A

M

G

G

Y

R

A

L

G

G

E

K

A

P

K

E

D

D

V

I

G

A

E

N

C

A

V

Y

A

L

F

F

L

L

A

A

S

S

D

H

G

E

A

S

R

D

Y

Y

I

V

N

N

G

G

E

H

V

V

I

L

N

H

V

V

G

D

G

G

M

I

V

M

L

M

active site: G18 (= G16), N111 (= N112), S139 (= S140), Q149 (= Q149), Y152 (= Y152), K156 (= K156)
binding acetoacetyl-coenzyme a: D93 (= D94), K98 (= K99), S139 (= S140), N146 (= N146), V147 (= V147), Q149 (= Q149), Y152 (= Y152), F184 (= F184), M189 (= M189), K200 (≠ I200)
binding 1,4-dihydronicotinamide adenine dinucleotide: G14 (= G12), N17 (≠ R15), G18 (= G16), I19 (= I17), D38 (= D36), F39 (≠ R37), V59 (≠ C60), D60 (≠ N61), V61 (≠ I62), N87 (= N88), A88 (= A89), G89 (= G90), I90 (= I91), T137 (≠ I138), S139 (= S140), Y152 (= Y152), K156 (= K156), P182 (= P182), F184 (= F184), T185 (≠ I185), T187 (= T187), M189 (= M189)

3sj7A Structure of beta-ketoacetyl-coa reductase (fabg) from staphylococcus aureus complex with NADPH (see paper)
46% identity, 98% coverage: 5:244/244 of query aligns to 1:239/239 of 3sj7A

query
sites
3sj7A

S

T

K

K

T

S

A

A

I

L

V

V

T

T

G
|
G

A

A

A
x
S

R
|
R

G
|
G

I
|
I

G

G

F

R

G

S

I

I

A

A

Q

L

V

Q

L

L

A

A

R

E

E

E

G

G

A

Y

R

N

V

V

I

A

I

V

-
x
N

-
x
Y

A
|
A

D
x
G

R
x
S

D

K

A

E

H

K

G

A

E

E

A

A

A

V

A

E

S

E

L

I

R

K

E

A

S

K

G

G

A

V

Q

D

A

S

L

F

F

A

I

I

S

Q

C
x
A

N
|
N

I
x
V

G

A

D

D

K

A

A

D

Q

E

V

V

E

K

A

A

L

M

F

I

S

K

Q

E

A

V

E

V

E

S

A

Q

F

F

G

G

A

S

V

L

D

D

I

V

V

L

V

V

N

N

N
|
N

A
|
A

G

G

I

I

N

T

R

R

D

D

A

N

M

L

L

L

H

M

K

R

L

M

S

K

E

E

A

Q

D

E

W

W

D

D

T

D

V

V

I

I

D

D

V
x
T

N

N

L

L

K

K

G

G

T

V

F

F

L

N

C

C

M

I

Q

Q

Q

K

A

A

A

T

I

P

R

Q

M

M

R

L

E

R

R

Q

G

R

A

S

G

G

R

A

I

I

N

N

I

L

A

S

S
|
S

-

V

A

V

S

G

W

A

L

V

G

G

N

N

V

P

G

G

Q
|
Q

T

A

N

N

Y
|
Y

S

V

A

A

S

T

K
|
K

A

A

G

G

V

V

V

I

G

G

M

L

T

T

K

K

T

S

A

A

C

A

R

R

E

E

L

L

A

A

K

S

K

R

G

G

V

I

T

T

V

V

N

N

A

A

I

V

C

A

P
|
P

G
|
G

F

F

I

I

D

V

T

S

D

D

M

M

T

T

R

D

G

E

V

L

P

K

E

E

N

Q

V

-

W

-

Q

-

I

-

M

M

I

L

N

T

K

Q

I

I

P

P

A

L

G

A

Y

R

A

F

G

G

E

Q

A

D

K

T

D

D

V

I

G

A

E

N

C

T

V

V

A

A

F

F

L

L

A

A

S

S

D

D

G

K

A

A

R

K

Y

Y

I

I

N

T

G

G

E

Q

V

T

I

I

N

H

V

V

G

N

G

G

M

M

V

Y

L

M

active site: G12 (= G16), S138 (= S140), Q148 (= Q149), Y151 (= Y152), K155 (= K156)
binding nadph dihydro-nicotinamide-adenine-dinucleotide phosphate: G8 (= G12), S10 (≠ A14), R11 (= R15), I13 (= I17), N31 (vs. gap), Y32 (vs. gap), A33 (= A35), G34 (≠ D36), S35 (≠ R37), A58 (≠ C60), N59 (= N61), V60 (≠ I62), N86 (= N88), A87 (= A89), T109 (≠ V111), S138 (= S140), Y151 (= Y152), K155 (= K156), P181 (= P182), G182 (= G183)

Q9KJF1 (2S)-[(R)-hydroxy(phenyl)methyl]succinyl-CoA dehydrogenase subunit BbsD; (S,R)-2-(alpha-hydroxybenzyl)succinyl-CoA dehydrogenase subunit BbsD; EC 1.1.1.429 from Thauera aromatica (see 2 papers)
45% identity, 100% coverage: 1:244/244 of query aligns to 1:245/248 of Q9KJF1

query
sites
Q9KJF1

M
|
M

K

G

L

I

A

Q

S

N

K

R

T

V

A

A

I

L

V

I

T

T

G

G

A

S

A

A

R
x
S

G

G

I

M

G

G

F

K

G

Q

I

T

A

A

Q

L

V

R

L

F

A

A

R

E

E

Q

G

G

A

A

R

A

V

V

I

V

I

I

A

N

D
|
D

R

I

D

D

A

A

H

E

G

K

-

V

E

R

A

A

A

T

A

V

A

D

S

E

L

F

R

S

E

A

S

R

G

G

A

H

Q

R

A

V

L

L

F

G

I

A

S

V

C

A

N
x
D

I
|
I

G

G

D

N

K

K

A

A

Q

A

V

V

E

D

A

G

L

M

F

V

S

K

Q

Q

A

T

E

I

E

D

A

A

F

F

G

G

A

R

V

I

D

D

I

I

V

L

V

V

N

N

N
|
N

A

A

G

G

I

M

N

E

R

R

D

A

A

G

M

A

L

L

H

R

K

K

L

L

S

S

E

E

A

A

D

D

W

W

D

D

T

V

V

T

I

I

D

N

V

V

N

N

L

L

K

K

G

G

T

T

F

F

L

L

C

C

M

T

Q

Q

Q

A

A

V

A

H

I

G

R

H

M

M

R

V

E

E

R

N

G

K

A

H

G

G

R

R

I

I

I

V

N

N

I

I

A

A

S

S

A

R

S

A

W

W

L

L

G

G

N

G

V

A

G

G

Q

Q

T

T

N

P

Y
|
Y

S

S

A

S

S

A

K
|
K

A

A

G

G

V

V

G

G

M

M

T

T

K

R

T

A

A

L

C

A

R

I

E

E

L

L

A

G

K

R

K

A

G

G

V

I

T

T

V

V

N

N

A

C

I

V

C

A

P

P

G

G

F

L

I

I

D

H

T

T

D

P

M

M

T

W

R

D

G

E

V

L

P

P

E

E

N

K

V

D

W

Q

Q

Q

I

F

M

L

I

L

N

S

K

R

I

Q

P

P

A

T

G

G

Y

K

A

L

G

G

E

E

A

P

K

D

D

D

V

I

G

A

E

N

C

T

V

L

A

L

F

F

L

L

A

A

S

D

D

D

G

D

A

S

R

G

Y

F

I

V

N

T

G

G

E

Q

V

V

I

L

N

Y

V

V

G

C

G

G

M

R

V

S

L

L

M1 (= M1) modified: Initiator methionine, Removed
S15 (≠ R15) binding
D36 (= D36) binding
D62 (≠ N61) binding
I63 (= I62) binding
N89 (= N88) binding
Y153 (= Y152) binding
K157 (= K156) binding

7pcsB Structure of the heterotetrameric sdr family member bbscd (see paper)
45% identity, 99% coverage: 3:244/244 of query aligns to 2:244/247 of 7pcsB

query
sites
7pcsB

L

I

A

Q

S

N

K

R

T

V

A

A

I

L

V

I

T

T

G
|
G

A

S

A

A

R

S

G

G

I
x
M

G

G

F

K

G

Q

I

T

A

A

Q

L

V

R

L

F

A

A

R

E

E

Q

G

G

A

A

R

A

V

V

I

V

I

I

A

N

D
|
D

R
x
I

D

D

A

A

H

E

G

K

-

V

E

R

A

A

A

T

A

V

A

D

S

E

L

F

R

S

E

A

S

R

G

G

A

H

Q

R

A

V

L

L

F

G

I

A

S

V

C

A

N

D

I
|
I

G

G

D

N

K

K

A

A

Q

A

V

V

E

D

A

G

L

M

F

V

S

K

Q

Q

A

T

E

I

E

D

A

A

F

F

G

G

A

R

V

I

D

D

I

I

V

L

V

V

N

N

N
|
N

A

A

G
|
G

I

M

N

E

R

R

D

A

A

G

M

A

L

L

H

R

K

K

L

L

S

S

E

E

A

A

D

D

W

W

D

D

T

V

V

T

I

I

D

N

V

V

N

N

L

L

K

K

G

G

T

T

F

F

L

L

C

C

M

T

Q

Q

Q

A

A

V

A

H

I

G

R

H

M

M

R

V

E

E

R

N

G

K

A

H

G

G

R

R

I

I

I

V

N

N

I
|
I

A

A

S
|
S

A

R

S

A

W

W

L

L

G

G

N

G

V

A

G

G

Q

Q

T

T

N

P

Y
|
Y

S

S

A

S

S

A

K
|
K

A

A

G

G

V

V

G

G

M

M

T

T

K

R

T

A

A

L

C

A

R

I

E

E

L

L

A

G

K

R

K

A

G

G

V

I

T

T

V

V

N

N

A

C

I

V

C

A

P

P

G

G

F

L

I
|
I

D

H

T

T

D

P

M

M

T

W

R

D

G

E

V

L

P

P

E

E

N

K

V

D

W

Q

Q

Q

I

F

M

L

I

L

N

S

K

R

I

Q

P

P

A

T

G

G

Y

K

A

L

G

G

E

E

A

P

K

D

D

D

V

I

G

A

E

N

C

T

V

L

A

L

F

F

L

L

A

A

S

D

D

D

G

D

A

S

R

G

Y

F

I

V

N

T

G

G

E

Q

V

V

I

L

N

Y

V

V

G

C

G

G

M

R

V

S

L

L

binding nicotinamide-adenine-dinucleotide: G11 (= G12), M16 (≠ I17), D35 (= D36), I36 (≠ R37), I62 (= I62), N88 (= N88), G90 (= G90), I138 (= I138), S140 (= S140), Y152 (= Y152), K156 (= K156), I185 (= I185)

3op4A Crystal structure of putative 3-ketoacyl-(acyl-carrier-protein) reductase from vibrio cholerae o1 biovar eltor str. N16961 in complex with NADP+ (see paper)
42% identity, 100% coverage: 1:244/244 of query aligns to 4:246/247 of 3op4A

query
sites
3op4A

M

M

K

N

L

L

A

E

S

G

K

K

T

V

A

A

I

L

V

V

T

T

G
|
G

A

A

A
x
S

R
|
R

G

G

I
|
I

G

G

F

K

G

A

I

I

A

A

Q

E

V

L

L

L

A

A

R

E

E

R

G

G

A

A

R

K

V

V

I

I

I

-

A

-

D

-

R

-

D

-

A

-

H

-

G

-

E

-

A

-

A

G

A

T

S

A

L
x
T

R

S

E

E

S

S

G

G

A

A

Q

Q

A

A

L

I

F

S

-

D

-

Y

-

L

-

G

-

D

-

N

-

G

-

K

-

G

I

M

S

A

C

L

N
|
N

I
x
V

G

T

D

N

K

P

A

E

Q

S

V

I

E

E

A

A

L

V

F

L

S

K

Q

A

A

I

E

T

E

D

A

E

F

F

G

G

A

G

V

V

D

D

I

I

V

L

V

V

N

N

N
|
N

A
|
A

G

G

I
|
I

N

T

R

R

D

D

A

N

M

L

L

L

H

M

K

R

L

M

S

K

E

E

A

E

D

E

W

W

D

S

T

D

V

I

I

M

D

E

V

T

N

N

L

L

K

T

G

S

T

I

F

F

L

-

C

R

M

L

Q

S

Q

K

A

A

A

V

I

L

R

R

-

G

M

M

R

M

E

K

R

K

G

R

A

Q

G

G

R

R

I

I

N

N

I
x
V

A

G

S
|
S

A

V

-

V

S

G

W

T

L

M

G

G

N

N

V

A

G

G

Q

Q

T

A

N

N

Y
|
Y

S

A

A

A

S

A

K
|
K

A

A

G

G

V

V

V

I

G

G

M

F

T

T

K

K

T

S

A

M

C

A

R

R

E

E

L

V

A

A

K

S

K

R

G

G

V

V

T

T

V

V

N

N

A

T

I

V

C

A

P
|
P

G
|
G

F

F

I
|
I

D

E

T
|
T

D

D

M
|
M

T

T

R

K

G

A

V

L

P

N

E

D

N

E

V

Q

W

R

Q

T

I

A

M

T

I

L

N

A

K

Q

I

V

P

P

A

A

G

G

Y

R

A

L

G

G

E

D

A

P

K

R

D

E

V

I

G

A

E

S

C

A

V

V

A

A

F

F

L

L

A

A

S

S

D

P

G

E

A

A

R

A

Y

Y

I

I

N

T

G

G

E

E

V

T

I

L

N

H

V

V

G

N

G

G

M

M

V

Y

L

M

binding nadp nicotinamide-adenine-dinucleotide phosphate: G15 (= G12), S17 (≠ A14), R18 (= R15), I20 (= I17), T40 (≠ L48), N62 (= N61), V63 (≠ I62), N89 (= N88), A90 (= A89), I92 (= I91), V139 (≠ I138), S141 (= S140), Y154 (= Y152), K158 (= K156), P184 (= P182), G185 (= G183), I187 (= I185), T189 (= T187), M191 (= M189)

1edoA The x-ray structure of beta-keto acyl carrier protein reductase from brassica napus complexed with NADP+ (see paper)
43% identity, 98% coverage: 5:244/244 of query aligns to 1:244/244 of 1edoA

query
sites
1edoA

S

S

K

P

T

V

A

V

I

V

V

V

T

T

G
|
G

A

A

A
x
S

R
|
R

G
|
G

I
|
I

G

G

F

K

G

A

I

I

A

A

Q

L

V

S

L

L

A

G

R

K

E

A

G

G

A

C

R

K

V

V

I

L

I

V

-
x
N

-
x
Y

A
|
A

D
x
R

R
x
S

D

A

A

K

H

A

G

A

E

E

A

E

A

V

A

S

A

K

S

Q

L

I

R

E

E

A

S

Y

G

G

A

G

Q

Q

A

A

L

I

F

T

I

F

S

G

C

G

N
x
D

I
x
V

G

S

D

K

K

E

A

A

Q

D

V

V

E

E

A

A

L

M

F

M

S

K

Q

T

A

A

E

I

E

D

A

A

F

W

G

G

A

T

V

I

D

D

I

V

V

V

N

N

N
|
N

A
|
A

G

G

I

I

N

T

R

R

D

D

A

T

M

L

L

L

H

I

K

R

L

M

S

K

E

K

A

S

D

Q

W

W

D

D

T

E

V

V

I

I

D

D

V

L

N

N

L

L

K

T

G

G

T

V

F

F

L

L

C

C

M

T

Q

Q

Q

A

A

A

A

T

I

K

R

I

M

M

R

M

E

K

R

K

G

R

A

K

G

G

R

R

I

I

N

N

I

I

A

A

S
|
S

A

V

S

V

W

G

L

L

-

I

G

G

N

N

V

I

G

G

Q

Q

T

A

N

N

Y
|
Y

S

A

A

A

S

A

K
|
K

A

A

G

G

V

V

V

I

G

G

M

F

T

S

K

K

T

T

A

A

C

A

R

R

E

E

L

G

A

A

K

S

K

R

G

N

V

I

T

N

V

V

N

N

A

V

I

V

C

C

P
|
P

G
|
G

F

F

I
|
I

D

A

T
x
S

D

D

M
|
M

T

T

R

A

G

K

V

L

P

G

E

E

N

D

V

M

W

E

Q

K

I

K

M

I

I

L

N

G

K

T

I

I

P

P

A

L

G

G

Y

R

A

T

G

G

E

Q

A

P

K

E

D

N

V

V

G

A

E

G

C

L

V

V

A

E

F

F

L

L

A

A

-

L

S

S

D

P

G

A

A

A

R

S

Y

Y

I

I

N

T

G

G

E

Q

V

A

I

F

N

T

V

I

G

D

G

G

M

I

V

A

L

I

active site: G12 (= G16), S138 (= S140), Y151 (= Y152), K155 (= K156)
binding nadp nicotinamide-adenine-dinucleotide phosphate: G8 (= G12), S10 (≠ A14), R11 (= R15), I13 (= I17), N31 (vs. gap), Y32 (vs. gap), A33 (= A35), R34 (≠ D36), S35 (≠ R37), D59 (≠ N61), V60 (≠ I62), N86 (= N88), A87 (= A89), S138 (= S140), Y151 (= Y152), K155 (= K156), P181 (= P182), G182 (= G183), I184 (= I185), S186 (≠ T187), M188 (= M189)

4dmmB 3-oxoacyl-[acyl-carrier-protein] reductase from synechococcus elongatus pcc 7942 in complex with NADP
45% identity, 100% coverage: 1:244/244 of query aligns to 1:239/240 of 4dmmB

query
sites
4dmmB

M

L

K

P

L

L

A

T

S

D

K

R

T

I

A

A

I

L

V

V

T

T

G
|
G

A

A

A
x
S

R
|
R

G
|
G

I
|
I

G

G

F

R

G

A

I

I

A

A

Q

L

V

E

L

L

A

A

R

A

E

A

G

G

A

A

R

K

V

V

I

A

I

V

A

N

D

Y

R
x
A

D
x
S

A
x
S

H

A

G

G

E

A

A

A

A

D

A

E

A

V

S

V

L

A

R

A

E

I

S

A

G

A

A

A

Q

G

A

G

L

E

F

A

I

F

-

A

-

V

S

K

C
x
A

N
x
D

I
x
V

G

S

D

Q

K

E

A

S

Q

E

V

V

E

E

A

A

L

L

F

F

S

A

Q

A

A

V

E

I

E

E

A

R

F

W

G

G

A

R

V

L

D

D

I

V

V

L

V

V

N

N

N
|
N

A
|
A

G

G

I

I

N

T

R

R

D

D

A

T

M

L

L

L

H

L

K

R

L

M

S

K

E

R

A

D

D

D

W

W

D

Q

T

S

V

V

I

L

D

D

V
x
L

N

N

L

L

K

G

G

G

T

V

F

F

L

L

C

C

M

S

Q

R

Q

A

A

A

I

K

R

I

M

M

R

L

E

K

R

Q

G

R

A

S

G

G

R

R

I

I

N

N

I
|
I

A

A

S
|
S

-

V

A

V

S

G

W

E

L

M

G

G

N

N

V

P

G

G

Q
|
Q

T

A

N

N

Y
|
Y

S

S

A

A

S

A

K
|
K

A

A

G

G

V

V

V

I

G

G

M

L

T

T

K

K

T

T

A

V

C

A

R

K

E

E

L

L

A

A

K

S

K

R

G

G

V

I

T

T

V

V

N

N

A

A

I

V

C

A

P
|
P

G
|
G

F

F

I
|
I

D

A

T
|
T

D

D

M
|
M

T

T

R

S

G

-

V

-

P

-

E

-

N

-

V

-

W

-

Q

-

I

-

M

L

I

L

N

E

K

V

I

I

P

P

A

L

G

G

Y

R

A

Y

G

G

E

E

A

A

K

A

D

E

V

V

G

A

E

G

C

V

V

V

A

R

F

F

L

L

A

A

S

A

D

D

-

P

G

A

A

A

R

A

Y

Y

I

I

N

T

G

G

E

Q

V

V

I

I

N

N

V

I

G

D

G

G

M

L

V

V

L

M

active site: G16 (= G16), S142 (= S140), Q152 (= Q149), Y155 (= Y152), K159 (= K156)
binding nadp nicotinamide-adenine-dinucleotide phosphate: G12 (= G12), S14 (≠ A14), R15 (= R15), G16 (= G16), I17 (= I17), A37 (≠ R37), S38 (≠ D38), S39 (≠ A39), A62 (≠ C60), D63 (≠ N61), V64 (≠ I62), N90 (= N88), A91 (= A89), L113 (≠ V111), I140 (= I138), S142 (= S140), Y155 (= Y152), K159 (= K156), P185 (= P182), G186 (= G183), I188 (= I185), T190 (= T187), M192 (= M189)

P0AEK2 3-oxoacyl-[acyl-carrier-protein] reductase FabG; 3-ketoacyl-acyl carrier protein reductase; Beta-Ketoacyl-acyl carrier protein reductase; Beta-ketoacyl-ACP reductase; EC 1.1.1.100 from Escherichia coli (strain K12) (see 2 papers)
41% identity, 100% coverage: 1:244/244 of query aligns to 1:243/244 of P0AEK2

query
sites
P0AEK2

M

M

K

N

L

F

A

E

S

G

K

K

T

I

A

A

I

L

V

V

T

T

G
|
G

A
|
A

A
x
S

R
|
R

G

G

I

I

G

G

F

R

G

A

I

I

A

A

Q

E

V

T

L

L

A

A

R

A

E

R

G

G

A

A

R

K

V

V

I

I

I

G

A

T

D

A

R
x
T

D

S

A

E

H

N

G

G

E

A

A

Q

A

A

A

I

A

S

S

D

L

Y

R

L

E

G

S

A

G

N

A

G

Q

K

A

G

L

L

F

M

I

L

S

-

C

-

N
|
N

I
x
V

G

T

D

D

K

P

A

A

Q

S

V

I

E

E

A

S

L

V

F

L

S

E

Q

K

A

I

E

R

E

A

A

E

F

F

G

G

A

E

V

V

D

D

I

I

V

L

V

V

N

N

N
|
N

A

A

G

G

I

I

N

T

R

R

D

D

A

N

M

L

L

L

H

M

K

R

L

M

S

K

E

D

A

E

D

E

W

W

D

N

T

D

V

I

I

I

D

E

V

T

N

N

L

L

K

S

G

S

T

V

F

F

L

R

C

L

M

S

Q

K

Q

A

A

V

A

M

I

R

R

A

M

M

R

M

E

K

R

K

G

R

A

H

G

G

R

R

I

I

N

T

I

I

A

G

S

S

A

V

-

V

S

G

W

T

L

M

G

G

N

N

V

G

G

G

Q

Q

T

A

N

N

Y
|
Y

S
x
A

A
|
A

S
x
A

K
|
K

A

A

G

G

V

L

V

I

G

G

M

F

T

S

K

K

T

S

A

L

C

A

R

R

E

E

L

V

A

A

K

S

K

R

G

G

V

I

T

T

V

V

N

N

A

V

I

V

C

A

P

P

G

G

F

F

I
|
I

D

E

T

T

D

D

M

M

T

T

R

R

G

A

V

L

P

S

E

D

N

D

V

Q

W

R

Q

A

I

G

M

I

I

L

N

A

K

Q

I

V

P

P

A

A

G

G

Y

R

A

L

G

G

E

G

A

A

K

Q

D

E

V

I

G

A

E

N

C

A

V

V

A

A

F

F

L

L

A

A

S

S

D

D

G

E

A

A

R

A

Y

Y

I

I

N

T

G

G

E
|
E

V

T

I

L

N

H

V

V

G

N

G

G

M

M

V

Y

L

M

GASR 12:15 (≠ GAAR 12:15) binding
T37 (≠ R37) binding
NV 59:60 (≠ NI 61:62) binding
N86 (= N88) binding
Y151 (= Y152) mutation to F: Defect in the affinity for NADPH.
YAAAK 151:155 (≠ YSASK 152:156) binding
A154 (≠ S155) mutation to T: Decreases in the thermolability of the reductase; when associated with K-233.
K155 (= K156) mutation to A: Defect in the affinity for NADPH.
I184 (= I185) binding
E233 (= E234) mutation to K: Decreases in the thermolability of the reductase; when associated with T-154.

P73574 3-oxoacyl-[acyl-carrier-protein] reductase; 3-ketoacyl-acyl carrier protein reductase; EC 1.1.1.100 from Synechocystis sp. (strain PCC 6803 / Kazusa) (see paper)
43% identity, 99% coverage: 3:244/244 of query aligns to 4:246/247 of P73574

query
sites
P73574

L

L

A

T

S

A

K

Q

T

V

A

A

I

L

V

V

T

T

G

G

A
|
A

A

S

R

R

G

G

I

I

G

G

F

K

G

A

I

T

A

A

Q

L

V

A

L

L

A

A

R

A

E

T

G

G

A

M

R

K

V

V

I

V

-

N

-

Y

A

A

D

Q

R

S

D

S

A

T

H

A

G

A

E

D

A

A

A

V

A

A

S

E

L

I

R

I

E

A

S

N

G

G

A

G

Q

E

A

A

L

I

F

A

I

V

S

Q

C

A

N

N

I

V

G

A

D

N

K

A

A

D

Q

E

V

V

E

D

A

Q

L

L

F

I

S

K

Q

T

A

T

E

L

E

D

A

K

F

F

G

S

A

R

V

I

D

D

I

V

V

L

V

V

N

N

A

A

G

G

I

I

N

T

R

R

D

D

A

T

M

L

L

L

H

L

K

R

L

M

S

K

E

L

A

E

D

D

W

W

D

Q

T

A

V

V

I

I

D

D

V

L

N

N

L

L

K

T

G

G

T

V

F

F

L

L

C

C

M

T

Q

K

Q

A

A

V

A

S

I

K

R

L

M

M

R

L

E

K

R

Q

G

K

A

S

G

G

R

R

I

I

N

N

I

I

A

T

S

S

-

V

A

A

S

G

W

M

L

M

G

G

N

N

V
x
P

G

G

Q

Q

T

A

N

N

Y

Y

S

S

A

A

S

A

K
|
K

A

A

G

G

V

V

V

I

G

G

M

F

T

T

K

K

T

T

A

V

C

A

R

K

E

E

L

L

A

A

K

S

K

R

G

G

V

V

T

T

V

V

N

N

A

A

I

V

C

A

P

P

G

G

F
|
F

I

I

D

A

T

T

D

D

M

M

T

T

R

E

G

N

V

L

-
x
N

P

A

E

E

N

P

V

I

W

L

Q

Q

I

F

M

-

I

-

N

-

K

-

I

I

P

P

A

L

G

A

Y

R

A

Y

G

G

E

Q

A

P

K

E

D

E

V

V

G

A

E

G

C

T

V

I

A

R

F

F

L

L

A

A

S

T

D

D

-

P

G

A

A

A

R

A

Y

Y

I

I

N

T

G

G

E

Q

V

T

I

F

N

N

V

V

G

D

G

G

M

M

V

V

L

M

A14 (= A13) mutation to G: 4.2-fold increase in activity on acetoacetyl-CoA.
P151 (≠ V147) mutation to F: 2.7-fold increase in activity on acetoacetyl-CoA.; mutation to V: 5.7-fold increase in activity on acetoacetyl-CoA.
K160 (= K156) mutation to A: Almost no activity on acetoacetyl-CoA.
F188 (= F184) mutation to Y: 3.3-fold increase in activity on acetoacetyl-CoA.
N198 (vs. gap) mutation to R: 3.5-fold increase in activity on acetoacetyl-CoA.

1q7bA The structure of betaketoacyl-[acp] reductase from e. Coli in complex with NADP+ (see paper)
42% identity, 98% coverage: 6:244/244 of query aligns to 5:242/243 of 1q7bA

query
sites
1q7bA

K

K

T

I

A

A

I

L

V

V

T

T

G
|
G

A

A

A
x
S

R
|
R

G
|
G

I

I

G

G

F

R

G

A

I

I

A

A

Q

E

V

T

L

L

A

A

R

A

E

R

G

G

A

A

R

K

V

V

I

I

I

G

A

T

D

A

R
x
T

D

S

A

E

H

N

G

G

E

A

A

Q

A

A

A

I

A

S

S

D

L

Y

R

L

E

G

S

A

G

N

A

G

Q

K

A

G

L

L

F

M

I

L

S

-

C

-

N
|
N

I
x
V

G

T

D

D

K

P

A

A

Q

S

V

I

E

E

A

S

L

V

F

L

S

E

Q

K

A

I

E

R

E

A

A

E

F

F

G

G

A

E

V

V

D

D

I

I

V

L

V

V

N

N

N
|
N

A
|
A

G
|
G

I
|
I

N

T

R

R

D

D

A

N

M

L

L

L

H

M

K

R

L

M

S

K

E

D

A

E

D
x
E

W

W

D

N

T

D

V

I

I

I

D

E

V

T

N

N

L

L

K

S

G

S

T

V

F

F

L

R

C

L

M

S

Q

K

Q

A

A

V

A

M

I

R

R

A

M

M

R

M

E

K

R

K

G

R

A

H

G

G

R

R

I

I

N

T

I

I

A

G

S
|
S

A

V

-

V

S

G

W

T

L

M

G

G

N

N

V

G

G

G

Q
|
Q

T

A

N

N

Y
|
Y

S

A

A

A

S

A

K
|
K

A

A

G

G

V

L

V

I

G

G

M

F

T

S

K

K

T

S

A

L

C

A

R

R

E

E

L

V

A

A

K

S

K

R

G

G

V

I

T

T

V

V

N

N

A

V

I

V

C

A

P
|
P

G
|
G

F

F

I
|
I

D

E

T

T

D

D

M

M

T

T

R

R

G

A

V

L

P

S

E

D

N

D

V

Q

W

R

Q

A

I

G

M

I

I

L

N

A

K

Q

I

V

P

P

A

A

G

G

Y

R

A

L

G

G

E

G

A

A

K

Q

D

E

V

I

G

A

E

N

C

A

V

V

A

A

F

F

L

L

A

A

S

S

D

D

G

E

A

A

R

A

Y

Y

I

I

N

T

G

G

E
|
E

V
x
T

I

L

N

H

V

V

G

N

G

G

M

M

V

Y

L

M

active site: G15 (= G16), E101 (≠ D104), S137 (= S140), Q147 (= Q149), Y150 (= Y152), K154 (= K156)
binding calcium ion: E232 (= E234), T233 (≠ V235)
binding nadp nicotinamide-adenine-dinucleotide phosphate: G11 (= G12), S13 (≠ A14), R14 (= R15), T36 (≠ R37), N58 (= N61), V59 (≠ I62), N85 (= N88), A86 (= A89), G87 (= G90), I88 (= I91), S137 (= S140), Y150 (= Y152), K154 (= K156), P180 (= P182), G181 (= G183), I183 (= I185)

P0A2C9 3-oxoacyl-[acyl-carrier-protein] reductase FabG; 3-ketoacyl-acyl carrier protein reductase; Beta-Ketoacyl-acyl carrier protein reductase; Beta-ketoacyl-ACP reductase; EC 1.1.1.100 from Salmonella typhimurium (strain LT2 / SGSC1412 / ATCC 700720) (see paper)
41% identity, 100% coverage: 1:244/244 of query aligns to 1:243/244 of P0A2C9

query
sites
P0A2C9

M

M

K

S

L

F

A

E

S

G

K

K

T

I

A

A

I

L

V

V

T

T

G

G

A

A

A

S

R

R

G

G

I

I

G

G

F

R

G

A

I

I

A

A

Q

E

V

T

L

L

A

V

R

A

E

R

G

G

A

A

R

K

V

V

I

I

I

-

A

-

D

-

R

G

D

T

A

A

H

T

G

S

E

E

A

N

A

G

A

A

A

K

S

N

L

I

R

S

E

D

S

Y

-

L

G

G

A

A

Q

N

A

G

L

K

F

G

I

L

S

M

C

L

N

N

I

V

G

T

D

D

K

P

A

A

Q

S

V

I

E

E

A

S

L

V

F

L

S

E

Q

N

A

I

E

R

E

A

A

E

F

F

G

G

A

E

V

V

D

D

I

I

V

L

V

V

N

N

A

A

G

G

I

I

N

T

R

R

D

D

A

N

M

L

L

L

H

M

K

R

L

M

S

K

E

D

A

D

D

E

W

W

D

N

T

D

V

I

I

I

D

E

V

T

N

N

L

L

K

S

G

S

T

V

F

F

L

R

C

L

M

S

Q

K

Q

A

A

V

A

M

I

R

R

A

M
|
M

R

M

E

K

R

K

G

R

A

C

G

G

R

R

I

I

N

T

I

I

A

G

S

S

A

V

-

V

S

G

W

T

L

M

G

G

N

N

V

A

G

G

Q

Q

T

A

N

N

Y

Y

S

A

A

A

S

A

K

K

A

A

G

G

V

L

V

I

G

G

M

F

T

S

K

K

T

S

A

L

C

A

R

R

E

E

L

V

A

A

K

S

K

R

G

G

V

I

T

T

V

V

N

N

A

V

I

V

C

A

P

P

G

G

F

F

I

I

D

E

T

T

D

D

M

M

T

T

R

R

G

A

V

L

P

S

E

D

N

D

V

Q

W

R

Q

A

I

G

M

I

I

L

N

A

K

Q

I

V

P

P

A

A

G

G

Y

R

A

L

G

G

E

G

A

A

K

Q

D

E

V

I

G

A

E

S

C

A

V

V

A

A

F

F

L

L

A
|
A

S
|
S

D

D

G

E

A

A

R

S

Y

Y

I

I

N

T

G

G

E

E

V

T

I

L

N

H

V

V

G

N

G

G

M

M

V

Y

L

M

M125 (= M127) mutation to I: Loss of the temperature-sensitive phenotype; when associated with T-223.
A223 (= A224) mutation to T: Loss of the temperature-sensitive phenotype; when associated with I-125.
S224 (= S225) mutation to F: Distorts the local conformation and prevent stacking around Phe-221. The S224F mutation would additionally disrupt the hydrogen bond formed between Ser-224 and Glu-226.

6t77A Crystal structure of klebsiella pneumoniae fabg(NADPH-dependent) NADP- complex at 1.75 a resolution (see paper)
41% identity, 100% coverage: 1:244/244 of query aligns to 1:243/244 of 6t77A

query
sites
6t77A

M

M

K

S

L

F

A

E

S

G

K

K

T

I

A

A

I

L

V

V

T

T

G
|
G

A

A

A
x
S

R
|
R

G
|
G

I

I

G

G

F

R

G

A

I

I

A

A

Q

E

V

T

L

L

A

V

R

A

E

R

G

G

A

A

R

K

V

V

I

I

I

-

A

-

D

-

R

-

D

-

A

-

H

-

G

G

E

T

A

A

A
x
T

A

S

A

-

S

-

L

-

R

-

E

E

S

S

G

G

A

A

Q

Q

A

A

L

I

F

S

-

D

-

Y

-

L

-

G

-

A

-

N

-

G

-

K

-

G

I

L

S

M

C
x
L

N
|
N

I
x
V

G

T

D

D

K

P

A

A

Q

S

V

I

E

E

A

S

L

V

F

L

S

E

Q

N

A

V

E

R

E

A

A

E

F

F

G

G

A

E

V

V

D

D

I

I

V

L

V

V

N

N

A
|
A

G
|
G

I
|
I

N

T

R

R

D

D

A

N

M

L

L

L

H

M

K

R

L

M

S

K

E

D

A

D

D

E

W

W

D

N

T

D

V

I

I

I

D

E

V

T

N

N

L

L

K

S

G

S

T

V

F

F

L

R

C

L

M

S

Q

K

Q

A

A

V

A

M

I

R

R

A

M

M

R

M

E

K

R

K

G

R

A

H

G

G

R

R

I

I

N

T

I

I

A

G

S
|
S

A

V

-

V

S

G

W

T

L

M

G

G

N

N

V

A

G

G

Q

Q

T

A

N

N

Y
|
Y

S

A

A

A

S

A

K

K

A

A

G

G

V

L

V

I

G

G

M

F

T

S

K

K

T

S

A

L

C

A

R

R

E

E

L

V

A

A

K

S

K

R

G

G

V

I

T

T

V

V

N

N

A

V

I

V

C

A

P

P

G

G

F

F

I

I

D

E

T

T

D

D

M

M

T

T

R

R

G

A

V

L

P

T

E

D

N

E

V

Q

W

R

Q

A

I

G

M

T

I

L

N

A

K

A

I

V

P

P

A

A

G

G

Y

R

A

L

G

G

E

T

A

P

K

N

D

E

V

I

G

A

E

S

C

A

V

V

A

A

F

F

L

L

A

A

S

S

D

D

G

E

A

A

R

S

Y

Y

I

I

N

T

G

G

E

E

V

T

I

L

N

H

V

V

G

N

G

G

M

M

V

Y

L

M

active site: G16 (= G16), S138 (= S140), Y151 (= Y152)
binding nadp nicotinamide-adenine-dinucleotide phosphate: G12 (= G12), S14 (≠ A14), R15 (= R15), T37 (≠ A44), L58 (≠ C60), N59 (= N61), V60 (≠ I62), A87 (= A89), G88 (= G90), I89 (= I91)

1q7cA The structure of betaketoacyl-[acp] reductase y151f mutant in complex with NADPH fragment (see paper)
41% identity, 98% coverage: 6:244/244 of query aligns to 5:242/243 of 1q7cA

query
sites
1q7cA

K

K

T

I

A

A

I

L

V

V

T

T

G
|
G

A

A

A
x
S

R
|
R

G
|
G

I

I

G

G

F

R

G

A

I

I

A

A

Q

E

V

T

L

L

A

A

R

A

E

R

G

G

A

A

R

K

V

V

I

I

I

G

A

T

D
x
A

R
x
T

D

S

A

E

H

N

G

G

E

A

A

Q

A

A

A

I

A

S

S

D

L

Y

R

L

E

G

S

A

G

N

A

G

Q

K

A

G

L

L

F

M

I
x
L

S

-

C

-

N
|
N

I
x
V

G

T

D

D

K

P

A

A

Q

S

V

I

E

E

A

S

L

V

F

L

S

E

Q

K

A

I

E

R

E

A

A

E

F

F

G

G

A

E

V

V

D

D

I

I

V

L

V

V

N

N

A

A

G
|
G

I
|
I

N

T

R

R

D

D

A

N

M

L

L

L

H

M

K

R

L

M

S

K

E

D

A

E

D

E

W

W

D

N

T

D

V

I

I

I

D

E

V

T

N

N

L

L

K

S

G

S

T

V

F

F

L

R

C

L

M

S

Q

K

Q

A

A

V

A

M

I

R

R

A

M

M

R

M

E

K

R

K

G

R

A

H

G

G

R

R

I

I

N

T

I

I

A

G

S
|
S

A

V

-

V

S

G

W

T

L

M

G

G

N

N

V

G

G

G

Q
|
Q

T

A

N

N

Y
x
F

S

A

A

A

S

A

K
|
K

A

A

G

G

V

L

V

I

G

G

M

F

T

S

K

K

T

S

A

L

C

A

R

R

E

E

L

V

A

A

K

S

K

R

G

G

V

I

T

T

V

V

N

N

A

V

I

V

C

A

P

P

G

G

F

F

I

I

D

E

T

T

D

D

M

M

T

T

R

R

G

A

V

L

P

S

E

D

N

D

V

Q

W

R

Q

A

I

G

M

I

I

L

N

A

K

Q

I

V

P

P

A

A

G

G

Y

R

A

L

G

G

E

G

A

A

K

Q

D

E

V

I

G

A

E

N

C

A

V

V

A

A

F

F

L

L

A

A

S

S

D

D

G

E

A

A

R

A

Y

Y

I

I

N

T

G

G

E

E

V

T

I

L

N

H

V

V

G

N

G

G

M

M

V

Y

L

M

active site: G15 (= G16), S137 (= S140), Q147 (= Q149), F150 (≠ Y152), K154 (= K156)
binding nadph dihydro-nicotinamide-adenine-dinucleotide phosphate: G11 (= G12), S13 (≠ A14), R14 (= R15), A35 (≠ D36), T36 (≠ R37), L57 (≠ I58), N58 (= N61), V59 (≠ I62), G87 (= G90), I88 (= I91)

4i08A Crystal structure of beta-ketoacyl-acyl carrier protein reductase (fabg) from vibrio cholerae in complex with NADPH (see paper)
41% identity, 100% coverage: 1:244/244 of query aligns to 4:242/243 of 4i08A

query
sites
4i08A

M

M

K

N

L

L

A

E

S

G

K

K

T

V

A

A

I

L

V

V

T

T

G
|
G

A

A

A
x
S

R
|
R

G
|
G

I
|
I

G

G

F

K

G

A

I

I

A

A

Q

E

V

L

L

L

A

A

R

E

E

R

G

G

A

A

R

K

V

V

I

I

I

-

A

-

D

-

R

-

D

-

A

-

H

-

G

-

E

-

A

-

A

G

A

T

S

A

L
x
T

R

S

E

E

S

S

G

G

A

A

Q

Q

A

A

L

I

F

S

-

D

-

Y

-

L

-

G

-

D

-

N

-

G

-

K

-

G

I

M

S

A

C

L

N
|
N

I
x
V

G

T

D

N

K

P

A

E

Q

S

V

I

E

E

A

A

L

V

F

L

S

K

Q

A

A

I

E

T

E

D

A

E

F

F

G

G

A

G

V

V

D

D

I

I

V

L

V

V

N

N

N
|
N

A
|
A

G

G

I

I

N

T

R

R

D

D

A

N

M

L

L

L

H

M

K

R

L

M

S

K

E

E

A

E

D

E

W

W

D

S

T

D

V

I

I

M

D

E

V

T

N
|
N

L

L

K

T

G

S

T

I

F

F

L

-

C

R

M

L

Q

S

Q

K

A

A

A

V

I

L

R

R

-

G

M

M

R

M

E

K

R

K

G

R

A

Q

G

G

R

R

I

I

N

N

I

V

A
x
G

S
|
S

A

V

-

V

S

G

W

T

L

M

G

G

N

N

V

A

G

G

Q
|
Q

T

A

N

N

Y
|
Y

S

A

A

A

S

A

K
|
K

A

A

G

G

V

V

V

I

G

G

M

F

T

T

K

K

T

S

A

M

C

A

R

R

E

E

L

V

A

A

K

S

K

R

G

G

V

V

T

T

V

V

N

N

A

T

I

V

C

A

P
|
P

G
|
G

F

F

I

I

D

E

T
|
T

D

D

M

M

T

N

R

-

G

-

V

-

P

-

E

D

N

E

V

Q

W

R

Q

T

I

A

M

T

I

L

N

A

K

Q

I

V

P

P

A

A

G

G

Y

R

A

L

G

G

E

D

A

P

K

R

D

E

V

I

G

A

E

S

C

A

V

V

A

A

F

F

L

L

A

A

S

S

D

P

G

E

A

A

R

A

Y

Y

I

I

N

T

G

G

E

E

V

T

I

L

N

H

V

V

G

N

G

G

M

M

V

Y

L

M

active site: G19 (= G16), N113 (= N112), S141 (= S140), Q151 (= Q149), Y154 (= Y152), K158 (= K156)
binding nadph dihydro-nicotinamide-adenine-dinucleotide phosphate: G15 (= G12), S17 (≠ A14), R18 (= R15), I20 (= I17), T40 (≠ L48), N62 (= N61), V63 (≠ I62), N89 (= N88), A90 (= A89), G140 (≠ A139), S141 (= S140), Y154 (= Y152), K158 (= K156), P184 (= P182), G185 (= G183), T189 (= T187)

4nbtA Crystal structure of fabg from acholeplasma laidlawii (see paper)
42% identity, 100% coverage: 2:244/244 of query aligns to 2:239/239 of 4nbtA

query
sites
4nbtA

K

K

L

L

A

E

S

G

K

K

T

V

A

A

I

V

V

I

T

T

G
|
G

A

G

A

A

R
x
K

G
|
G

I
x
L

G

G

F

Q

G

A

I

I

A

A

Q

L

V

A

L

Y

A

A

R

E

E

E

G

G

A

A

R

K

V

V

I

I

I

A

A

G

D
|
D

R

-

D

-

A

-

H

-

G

-

E

-

A

-

A
x
L

A

G

S

D

L

L

R

T

E

Y

S

S

G

H

A

P

Q

N

A

V

L

E

F

G

I

M

S

Y

C
x
L

N
|
N

I
x
V

G

T

D

D

K

V

A

T

Q

G

V

V

E

E

A

K

L

F

F

Y

S

Q

Q

S

A

V

E

I

E

D

A

K

F

Y

G

G

A

K

V

I

D

D

I

I

V

L

V

V

N

N

N
|
N

A
|
A

G
|
G

I

I

N

T

R

K

D

D

A

A

M

M

L

T

H

R

K

K

L

M

S

T

E

E

A

A

D

Q

W

W

D

D

T

A

V

V

I

I

D

D

V

V

N

N

L

L

K

K

G

G

T

V

F

F

L

N

C

L

M

T

Q

R

Q

L

A

V

A

G

I

P

R

Q

M

M

R

Q

E

T

R

N

G

G

A

Y

G

G

R

S

I

I

N

N

I
|
I

A

S

S
|
S

-

V

A

V

S

G

W

V

L

F

G

G

N

N

V

I

G

G

Q

Q

T

A

N

N

Y
|
Y

S

A

A

A

S

T

K
|
K

A

A

G

G

V

V

V

I

G

G

M

L

T

T

K

M

T

T

A

W

C

A

R

K

E

E

L

F

A

A

K

L

K

K

G

G

-

A

-

N

V

V

T

R

V

V

N

N

A

A

I

I

C

A

P
|
P

G
|
G

F

Y

I
|
I

D

M

T
|
T

D

D

M

I

T

L

R

K

G

T

V

V

P

P

E

Q

N

D

V

L

W

L

Q

D

-

K

-

F

-

A

-

L

I

T

M

M

I

L

N

N

K

R

I

L

P

-

A

-

G

-

Y

-

A

-

G

G

E

Q

A

P

K

E

D

E

V

I

G

A

E

K

C

V

V

A

A

L

F

F

L

L

A

A

S

S

D

D

G

D

A

A

R

S

Y

Y

I

V

N

T

G

G

E

Q

V

T

I

I

N

N

V

V

G

N

G

G

M

M

V

R

L

L

active site: G16 (= G16), S132 (= S140), Y145 (= Y152), K149 (= K156)
binding nicotinamide-adenine-dinucleotide: G12 (= G12), K15 (≠ R15), G16 (= G16), L17 (≠ I17), D36 (= D36), L37 (≠ A45), L52 (≠ C60), N53 (= N61), V54 (≠ I62), N80 (= N88), A81 (= A89), G82 (= G90), I130 (= I138), S132 (= S140), Y145 (= Y152), K149 (= K156), P177 (= P182), G178 (= G183), I180 (= I185), T182 (= T187)

7emgB Carbonyl reductase variant 4 (r123c/l209p/f183y/v61k) from serratia marcescens complexed with NADP+ (see paper)
40% identity, 98% coverage: 6:244/244 of query aligns to 5:242/243 of 7emgB

query
sites
7emgB

K

K

T

I

A

V

I

L

V

V

T

T

G
|
G

A

A

A
x
S

R
|
R

G

G

I

I

G

G

F

R

G

A

I

I

A

A

Q

E

V

T

L

F

A

V

R

A

E

R

G

G

A

A

R

K

V

V

I

I

-

G

I

T

A

A

D
x
T

R

S

D

E

A

S

H

G

G

A

E

E

A

A

A

I

A

S

A

G

S

Y

L

L

R

-

E

-

S

-

G

G

A

A

Q

N

A

G

L

K

F

G

I

F

S

M

C

L

N
|
N

I
x
V

G

K

D

D

K

A

A

Q

Q

S

V

I

E

D

A

S

L

V

F

L

S

A

Q

S

A

I

E

R

E

A

A

E

F

F

G

G

A

E

V

I

D

D

I

I

V

L

V

V

N

N

A

A

G

G

I
|
I

N

T

R

R

D

D

A

N

M

L

L

L

H

M

K

R

L

M

S

K

E

D

A

D

D

E

W

W

D

E

T

D

V

I

I

L

D

D

V

T

N

N

L

L

K

T

G

S

T

V

F

F

L

R

C

L

M

S

Q

K

Q

A

A

V

A

M

I

C

R

A

M

M

R

M

E

K

R

K

G

R

A

F

G

G

R

R

I

I

N

T

I

I

A

G

S

S

A

V

-

V

S

G

W

T

L

M

G

G

N

N

V

A

G

G

Q

Q

T

A

N

N

Y

Y

S

A

A

A

S

A

K

K

A

A

G

G

V

L

V

I

G

G

M

F

T

S

K

K

T

S

A

L

C

A

R

R

E

E

L

V

A

A

K

S

K

R

G

G

V

I

T

T

V

V

N

N

A

V

I

V

C

A

P

P

G

G

F

Y

I

I

D

E

T

T

D

D

M

M

T

T

R

R

G

A

V

L

P

T

E

D

N

D

V

Q

W

R

Q

A

I

G

M

I

I

L

N

S

K

S

I

V

P

P

A

A

G

N

Y

R

A

P

G

G

E

D

A

A

K

K

D

E

V

I

G

A

E

S

C

A

V

V

A

A

F

F

L

L

A

A

S

S

D

D

G

E

A

A

R

G

Y

Y

I

I

N

T

G

G

E

E

V

T

I

L

N

H

V

V

G

N

G

G

M

M

V

Y

L

M

binding nadph dihydro-nicotinamide-adenine-dinucleotide phosphate: G11 (= G12), S13 (≠ A14), R14 (= R15), T36 (≠ D36), N58 (= N61), V59 (≠ I62), I88 (= I91)

P71534 3-oxoacyl-[acyl-carrier-protein] reductase MabA; 3-ketoacyl-acyl carrier protein reductase; Acetoacetyl-CoA reductase; Beta-ketoacyl-ACP reductase; Beta-ketoacyl-acyl carrier protein reductase; EC 1.1.1.100; EC 1.1.1.36 from Mycolicibacterium smegmatis (strain ATCC 700084 / mc(2)155) (Mycobacterium smegmatis) (see paper)
42% identity, 98% coverage: 3:242/244 of query aligns to 21:251/255 of P71534

query
sites
P71534

L

F

A

V

S

S

K

R

T

S

A

V

I

L

V

V

T

T

G

G

A

G

A
x
N

R
|
R

G
|
G

I
|
I

G

G

F

L

G

A

I

I

A

A

Q

R

V

R

L

L

A

A

R

A

E

D

G

G

A

H

R

K

V

V

I

A

I

V

A

T

D

H

R
|
R

D

-

A

-

H

-

G

G

E

S

A

G

A

A

A

P

A

D

S

D

L

L

R

-

E

-

S

-

G

-

A

-

Q

-

A

-

L

F

F

G

I

V

S

Q

C

C

N
x
D

I
x
V

G

T

D

D

K

S

A

A

Q

A

V

V

E

D

A

R

L

A

F

F

S

K

Q

E

A

V

E

E

A

H

F

Q

G

G

A

P

V

V

D

E

I

V

V

L

V

V

N

A

N

N

A

A

G

G

I

I

N

S

R

K

D

D

A

A

M

F

L

L

H

M

K

R

L

M

S

T

E

E

A

E

D

R

W

F

D

E

T

E

V

V

I

I

D

N

V

T

N

N

L

L

K

T

G

G

T

A

F

F

L

R

C

C

M

A

Q

Q

Q

R

A

A

A

S

I

R

R

T

M

M

R

Q

E

R

R

K

G

R

A

F

G

G

R

R

I

I

N

F

I

I

A

G

S

S

A

V

S

S

W

G

L

M

G

W

N

G

V

I

G

G

-

N

Q

Q

T

A

N

N

Y
|
Y

S

A

A

A

S

A

K
|
K

A

A

G

G

V

L

V

I

G

G

M

M

T

A

K

R

T

S

A

I

C

S

R

R

E

E

L

L

A

A

K

K

K

A

G

G

V

V

T

T

V

A

N

N

A

V

I

V

C

A

P

P

G

G

F

Y

I
|
I

D

D

T

T

D

E

M

M

T

T

R

R

G

A

V

L

P

D

E

E

N
x
R

V

I

W

Q

Q

A

I

G

M

A

I

L

N

D

K

F

I

I

P

P

A

A

G

K

Y

R

A

V

G

G

E

T

A

A

K

E

D

E

V

V

G

A

E

G

C

A

V

V

A

S

F

F

L

L

A

A

S

S

D

E

G

D

A

A

R

S

Y

Y

I

I

N

A

G

G

E

A

V

V

I

I

N

P

V

V

G

D

G

G

M

M

NRGI 32:35 (≠ ARGI 14:17) binding
R55 (= R37) binding
DV 69:70 (≠ NI 61:62) binding
Y161 (= Y152) binding
K165 (= K156) binding
I194 (= I185) binding
R205 (≠ N196) binding

Query Sequence

>BWI76_RS23705 FitnessBrowser__Koxy:BWI76_RS23705
MKLASKTAIVTGAARGIGFGIAQVLAREGARVIIADRDAHGEAAAASLRESGAQALFISC
NIGDKAQVEALFSQAEEAFGAVDIVVNNAGINRDAMLHKLSEADWDTVIDVNLKGTFLCM
QQAAIRMRERGAGRIINIASASWLGNVGQTNYSASKAGVVGMTKTACRELAKKGVTVNAI
CPGFIDTDMTRGVPENVWQIMINKIPAGYAGEAKDVGECVAFLASDGARYINGEVINVGG
GMVL

Or try a new SitesBLAST search

SitesBLAST's Database

SitesBLAST's database includes (1) SwissProt entries with experimentally-supported functional features; and (2) protein structures with bound ligands, from the BioLip database.

by Morgan Price, Arkin group
Lawrence Berkeley National Laboratory