SitesBLAST
Comparing BWI76_RS23855 FitnessBrowser__Koxy:BWI76_RS23855 to proteins with known functional sites using BLASTp with E ≤ 0.001.
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Found 20 (the maximum) hits to proteins with known functional sites (download)
4f66A The crystal structure of 6-phospho-beta-glucosidase from streptococcus mutans ua159 in complex with beta-d-glucose-6-phosphate. (see paper)
62% identity, 99% coverage: 6:477/477 of query aligns to 5:478/478 of 4f66A
- active site: R86 (= R87), H131 (= H132), E177 (= E178), N180 (= N181), S312 (= S313), Y314 (= Y315), E376 (= E375)
- binding 6-O-phosphono-beta-D-glucopyranose: Q19 (= Q20), H131 (= H132), F132 (= F133), E177 (= E178), Y314 (= Y315), W350 (= W349), E376 (= E375), W424 (= W423), S431 (= S430), A432 (≠ F431), G433 (≠ T432), K439 (= K438), Y441 (= Y440)
4f79A The crystal structure of 6-phospho-beta-glucosidase mutant (e375q) in complex with salicin 6-phosphate (see paper)
62% identity, 99% coverage: 6:477/477 of query aligns to 5:478/478 of 4f79A
- active site: R86 (= R87), H131 (= H132), E177 (= E178), N180 (= N181), S312 (= S313), Y314 (= Y315), Q376 (≠ E375)
- binding 2-(hydroxymethyl)phenyl 6-O-phosphono-beta-D-glucopyranoside: Q19 (= Q20), H131 (= H132), F132 (= F133), E177 (= E178), Y314 (= Y315), W350 (= W349), Q376 (≠ E375), W424 (= W423), S431 (= S430), A432 (≠ F431), G433 (≠ T432), K439 (= K438), Y441 (= Y440)
4gpnB The crystal structure of 6-p-beta-d-glucosidase (e375q mutant) from streptococcus mutans ua150 in complex with gentiobiose 6-phosphate. (see paper)
62% identity, 99% coverage: 6:477/477 of query aligns to 3:476/476 of 4gpnB
- active site: R84 (= R87), H129 (= H132), E175 (= E178), N178 (= N181), S310 (= S313), Y312 (= Y315), Q374 (≠ E375)
- binding 6-O-phosphono-beta-D-glucopyranose: Q17 (= Q20), H129 (= H132), F130 (= F133), E175 (= E178), Y312 (= Y315), W348 (= W349), Q374 (≠ E375), W422 (= W423), S429 (= S430), A430 (≠ F431), G431 (≠ T432), K437 (= K438), Y439 (= Y440)
- binding beta-D-glucopyranose: E175 (= E178), W348 (= W349)
3qomA Crystal structure of 6-phospho-beta-glucosidase from lactobacillus plantarum (see paper)
57% identity, 100% coverage: 1:477/477 of query aligns to 4:478/479 of 3qomA
- active site: R90 (= R87), H135 (= H132), E181 (= E178), N184 (= N181), S316 (= S313), Y318 (= Y315), E376 (= E375)
- binding beta-D-glucopyranose: E181 (= E178), N184 (= N181), A244 (= A241), R268 (= R265), W350 (= W349)
- binding phosphate ion: Y28 (≠ W25), H65 (= H62), H202 (≠ Y199), P204 (≠ E201), G205 (≠ H202), W350 (= W349), A431 (≠ S430), A432 (≠ F431), S433 (≠ T432), K439 (= K438), Y441 (= Y440)
4ipnE The complex structure of 6-phospho-beta-glucosidase bgla-2 with thiocellobiose-6p from streptococcus pneumoniae (see paper)
48% identity, 99% coverage: 6:477/477 of query aligns to 3:462/463 of 4ipnE
4ipnB The complex structure of 6-phospho-beta-glucosidase bgla-2 with thiocellobiose-6p from streptococcus pneumoniae (see paper)
48% identity, 99% coverage: 6:477/477 of query aligns to 3:456/457 of 4ipnB
- active site: R79 (= R87), H124 (= H132), E170 (= E178), S173 (≠ N181), S300 (= S313), Y302 (= Y315), E351 (= E375)
- binding 6-O-phosphono-alpha-L-idopyranose: Q17 (= Q20), H124 (= H132), N169 (= N177), E351 (= E375), W402 (= W423), S409 (= S430), M410 (≠ F431), S411 (≠ T432)
- binding 4-thio-beta-D-glucopyranose: Y125 (≠ F133), E170 (= E178), S173 (≠ N181)
4zfmC Structure of gan1d-e170q in complex with cellobiose-6-phosphate
39% identity, 99% coverage: 5:477/477 of query aligns to 4:461/461 of 4zfmC
- active site: R76 (= R87), H120 (= H132), Q166 (≠ E178), I169 (≠ N181), N295 (≠ S313), Y297 (= Y315), E361 (= E375)
- binding 1,5-anhydro-6-O-phosphono-D-glucitol: Q19 (= Q20), H120 (= H132), Q166 (≠ E178), E361 (= E375), W408 (= W423), S415 (= S430), W416 (≠ F431), Y424 (= Y440)
- binding beta-D-glucopyranose: Q166 (≠ E178), Y297 (= Y315)
5okbB High resolution structure of native gan1d, a putative 6-phospho-beta- galactosidase from geobacillus stearothermophilus (see paper)
38% identity, 99% coverage: 5:477/477 of query aligns to 4:474/474 of 5okbB
4zepA Structure of gan1d, a 6-phospho-beta-galactosidase from geobacillus stearothermophilus, in complex with 6-phospho-glucose
38% identity, 99% coverage: 5:477/477 of query aligns to 4:474/474 of 4zepA
- active site: R76 (= R87), H120 (= H132), E166 (= E178), I169 (≠ N181), N295 (≠ S313), Y297 (= Y315), E374 (= E375)
- binding 6-O-phosphono-beta-D-glucopyranose: Q19 (= Q20), E166 (= E178), Y297 (= Y315), W348 (= W349), E374 (= E375), W421 (= W423), S428 (= S430), K435 (= K438), Y437 (= Y440)
8b80A The structure of gan1d w433a in complex with galactose-6p
38% identity, 99% coverage: 5:477/477 of query aligns to 4:474/474 of 8b80A
6z1mA Structure of an ancestral glycosidase (family 1) bound to heme (see paper)
36% identity, 99% coverage: 4:474/477 of query aligns to 1:422/423 of 6z1mA
- binding protoporphyrin ix containing fe: P164 (≠ I179), N165 (= N180), L194 (≠ Q216), L195 (≠ F217), L218 (= L240), L220 (≠ M242), N244 (≠ R265), F247 (= F268), K253 (≠ R274), Y256 (= Y277), L288 (≠ C307), R318 (≠ E362), Y323 (= Y367)
- binding magnesium ion: H346 (≠ N391), K409 (= K461)
5yifA Pyruvylated beta-d-galactosidase from bacillus sp. Hma207, e163a mutant pyruvylated beta-d-galactose complex (see paper)
33% identity, 99% coverage: 4:477/477 of query aligns to 1:459/459 of 5yifA
- binding (2R,4aR,6R,7R,8R,8aR)-2-methyl-6,7,8-tris(oxidanyl)-4,4a,6,7,8,8a-hexahydropyrano[3,2-d][1,3]dioxine-2-carboxylic acid: Q17 (= Q20), H118 (= H132), N162 (= N177), N295 (≠ S313), Y297 (= Y315), W337 (= W349), E364 (= E375), W410 (= W423), S417 (= S430), W418 (≠ F431), K424 (= K438)
8ivyA Beta-glucosidase bgla mutant e166q in complex with glucose (see paper)
33% identity, 99% coverage: 1:474/477 of query aligns to 1:447/451 of 8ivyA
4ptwA Halothermothrix orenii beta-glucosidase a, 2-deoxy-2-fluoro-glucose complex (see paper)
34% identity, 98% coverage: 4:470/477 of query aligns to 1:440/445 of 4ptwA
- active site: R74 (= R87), H118 (= H132), E163 (= E178), V166 (≠ R186), N291 (≠ S313), Y293 (= Y315), E351 (= E375)
- binding 2-deoxy-2-fluoro-alpha-D-glucopyranose: Q17 (= Q20), H118 (= H132), E163 (= E178), Y293 (= Y315), E351 (= E375), W398 (= W423), E405 (≠ S430), W406 (≠ F431)
4ptvA Halothermothrix orenii beta-glucosidase a, thiocellobiose complex (see paper)
34% identity, 98% coverage: 4:470/477 of query aligns to 1:440/445 of 4ptvA
- active site: R74 (= R87), H118 (= H132), E163 (= E178), V166 (≠ R186), N291 (≠ S313), Y293 (= Y315), E351 (= E375)
- binding beta-D-glucopyranose: W324 (= W349), E405 (≠ S430), Y408 (≠ T433)
- binding 4-thio-beta-D-glucopyranose: Q17 (= Q20), H118 (= H132), E163 (= E178), Y293 (= Y315), E351 (= E375), W398 (= W423), E405 (≠ S430), W406 (≠ F431)
6r4kA Structure of beta-glucosidase a from paenibacillus polymyxa complexed with a monovalent inhibitor (see paper)
31% identity, 99% coverage: 1:473/477 of query aligns to 1:442/448 of 6r4kA
- binding (2~{S},3~{S},4~{R})-2-[[4-[4-[2-[2-(2-azanylidenehydrazinyl)ethoxy]ethoxy]phenyl]-1,2,3-triazol-1-yl]methyl]pyrrolidine-3,4-diol: Q20 (= Q20), H121 (= H132), E166 (= E178), L173 (≠ F190), E352 (= E375), W398 (= W423), E405 (≠ S430), W406 (≠ F431)
6qwiA Structure of beta-glucosidase a from paenibacillus polymyxa complexed with multivalent inhibitors. (see paper)
31% identity, 99% coverage: 1:473/477 of query aligns to 1:442/448 of 6qwiA
- binding (2~{S},3~{S},4~{R})-2-[[4-[4-(2-ethoxyethoxy)phenyl]-1,2,3-triazol-1-yl]methyl]pyrrolidine-3,4-diol: Q20 (= Q20), H121 (= H132), W122 (≠ F133), E166 (= E178), L173 (≠ F190), W326 (= W349), E352 (= E375), W398 (= W423), E405 (≠ S430), W406 (≠ F431)
1bggB Glucosidase a from bacillus polymyxa complexed with gluconate (see paper)
31% identity, 98% coverage: 6:473/477 of query aligns to 5:441/447 of 1bggB
- active site: R76 (= R87), H120 (= H132), E165 (= E178), C168 (≠ R186), N293 (≠ S313), Y295 (= Y315), E351 (= E375)
- binding D-gluconic acid: Q19 (= Q20), H120 (= H132), W121 (≠ F133), N164 (= N177), E165 (= E178), Y295 (= Y315), E351 (= E375), W397 (= W423), E404 (≠ S430), W405 (≠ F431)
1e4iA 2-deoxy-2-fluoro-beta-d-glucosyl/enzyme intermediate complex of the beta-glucosidase from bacillus polymyxa (see paper)
31% identity, 98% coverage: 6:473/477 of query aligns to 5:441/447 of 1e4iA
- active site: R76 (= R87), H120 (= H132), E165 (= E178), C168 (≠ R186), N293 (≠ S313), Y295 (= Y315), E351 (= E375)
- binding 2-deoxy-2-fluoro-alpha-D-glucopyranose: Q19 (= Q20), H120 (= H132), N164 (= N177), E165 (= E178), Y295 (= Y315), E351 (= E375), W397 (= W423), E404 (≠ S430), W405 (≠ F431)
- binding 2,4-dinitrophenyl 2-deoxy-2-fluoro-beta-D-glucopyranoside: R136 (= R149), Q140 (≠ D153), V143 (= V156), L202 (= L222)
Q9FIW4 Beta-glucosidase 42; AtBGLU42; EC 3.2.1.21 from Arabidopsis thaliana (Mouse-ear cress) (see paper)
31% identity, 99% coverage: 6:475/477 of query aligns to 21:484/490 of Q9FIW4
- R342 (≠ H341) Important for substrate chain length specificity; mutation R->A,Y: Highest preference on cellotetraose and cellopentaose with increased affinities at subsite (+)3.
Query Sequence
>BWI76_RS23855 FitnessBrowser__Koxy:BWI76_RS23855
MKKIPLPKDFLWGGAVAAHQVEGGWNKDGKGPSICDVLTGGAHGVPREITQQVEPGKYYP
NHEAIDFHGRYKEDIKLFAEMGFKCFRTSIAWTRIFPLGDESQPNEEGLKFYDDMFDELL
KYNIEPVITLSHFEMPLHLVQQYGGWTNRKVVDFFVRFAEVVFERYKHKVKYWMTFNEIN
NQRNWRAPLFGYCCSGVVYTEHENPEETMYQVLHHQFVASALAVKAARQINPQMQVGCML
AMVPLYPYSCKPEDVMFAQESMRERYVFTDVQLRGYYPSYVLNEWERRGFSIKMEDGDAQ
ILREGTCAYLGFSYYMTNAVKAEGGSGDAISGFEGSVPNPHVKASDWGWQIDSVGLRYAL
CELYERYQKPLFIVENGFGAYDKVEEDGSINDDYRIDYLRAHVEEMIKAVTYDGVELMGY
TPWGCIDCVSFTTGQYSKRYGFIYVNKHDDGTGDMSRSRKKSFNWYKDVIASNGEKL
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SitesBLAST's Database
SitesBLAST's database includes
(1) SwissProt
entries with experimentally-supported functional features;
and (2) protein structures with bound ligands, from the
BioLip database.
by Morgan Price,
Arkin group
Lawrence Berkeley National Laboratory