SitesBLAST

Q9NRA2 Sialin; H(+)/nitrate cotransporter; H(+)/sialic acid cotransporter; AST; Membrane glycoprotein HP59; Solute carrier family 17 member 5; Vesicular excitatory amino acid transporter; VEAT from Homo sapiens (Human) (see 8 papers)
23% identity, 82% coverage: 12:367/433 of query aligns to 62:440/495 of Q9NRA2

query
sites
Q9NRA2

I

V

A

A

L

L

V

V

T

D

L

M

G

V

T

D

V

S

L

N

G

T

Y

T

L

L

T

E

R

D

N

N

T

R

V

T

A

S

A

K

A

A

A

C

P

P

T

E

L

H

M

S

E

A

E

P

L

I

H

K

I

V

S

H

T

H

Q

N

Q

Q

-

T

-

G

-

K

-

Y

-

Q

-

W

-

D

-

A

-

E

-

T

Y

Q

S

G

Y

W

I

I

I

L

A

G

A

S

Y

F

S

F

A

Y

A

G

Y

Y

T

I

V

I

M

T

Q

Q

P

I

V

P

A

G

G

G

Y

Y

V

V

L

A

D

S

V

K

L

I

G

G

T

G

K
|
K

I

M

G

L

Y

L

A

G

F

F

F

G

A

I

I

L

A

G

W

T

A

A

V

V

F

L

C

T

G

L

S

F

T

T

A

P

L

I

A

A

G

A

S

D

W

L

G

G

-

V

-

G

G

P

L

L

A

I

L

V

A

L

R

R

G

A

A

L

V

E

G

G

A

L

A

G

E

E

A

G

A

V

M

T

I

F

P

P

A

A

G

M

L
x
H

K

A

A

M

S

W

S

S

E

S

W

W

F

A

P

P

A

P

K

L

E

E

R

R

S

S

I

K

A
x
L

V
x
L

G

S

Y

I

F

S

N

Y

V

A

G

G

S

A

S

Q

I

L

G

G

A

T

M

V

I

I

A

S

P

L

P

P

L

L

V

S

V

G

W

I

A

I

I

C

V

Y

M

Y

H

M

S

N

W

W

Q

T

M

Y

A

V

F

F

I

Y

I

F

S

F

G

G

V

T

L

I

S

G

F

I

A

F

W

W

A

F

M

L

A

L

W

W

L

I

-

W

V

L

F

V

Y

S

K

D

H

T

P

P

R

Q

D

K

Q

H

K

K

K

R

L

I

T

S

E

H

E

Y

E

E

R

K

N

E

Y

Y

I
|
I

I
x
L

G

-

Q

-

E
x
S

S
|
S

H
x
L

H
x
R

Q
x
N

T

Q

N

L

N

S

G

S

K

Q

K

K

M

S

S

V

P

P

W

W

-

V

Q

P

I

I

L

L

R

K

N

S

R

L

Q

P

F

L

W

W

G

A

I

I

A

V

L

V

P

A

R

H

F

F

L

S

A

Y

E

N

P

W

A

T

W

F

G

Y

T

T

F

L

N

L

A

T

W

L

I

L

P

P

L

T

F

Y

M

M

F

K

K

E

V

I

Y

L

G

R

F

F

N

N

L

V

K

Q

E

E

I

N

A
x
G

M

F

F

L

A

S

W

S

M

L

P
|
P

M

Y

L

L

F

G

A

S

D

W

L

L

G

C

C

M

I

I

V

L

G

S

G

G

Y

Q

L

A

P

A

P

D

L

N

F

L

Q

R

R

A

W

K

F

W

G

N

V

F

N

S

L

T

I

L

V

C

S

V

R

R

K

R

M

I

V

F

V

S

T

L

M

I

G
|
G

-

M

-

I

-

G

-

P

A

A

L

V

L

F

M

L

I

V

G

A

P

A

G

G

M

F

I

I

G

G

L

C

F

D

T

Y

S

S

P

-

F

-

V

L

A

A

I

V

A

A

L

F

L

L

C

T

V

I

-

S

-

T

-

L

G

G

F

F

A

C

H

S

Q

S

A

G

L

F

S

S

G

I

A

N

L

H

I

L

T

D

L

I

S

A

S

P

D

S

V

Y

F

A

G

G

-

I

-

L

-

G

-

I

R

T

N

N

E

T

V

F

A

A

T

T

A

I

N

P

G

G

M

M

T

V

G

G

K136 (= K75) to E: in SD; alters intracellular localization, only partially targeted to lysosomes and mainly detected in LAMP1-negative vesicles and in the Golgi apparatus; completely devoid of L-aspartate and L-glutamate transporter activity, but retains appreciable H(+)-coupled sialic acid transporter activity; dbSNP:rs80338795
H183 (≠ L120) to R: in ISSD; alters intracellular localization, only partially targeted to lysosomes and mainly detected in LAMP1-negative vesicles and in the Golgi apparatus; abolishes H(+)-coupled sialic acid transporter activity; has normal L-aspartate and L-glutamate transporter activity; dbSNP:rs119491109
LL 198:199 (≠ AV 135:136) mutation to AA: Localizes in vesicular structures mainly concentrated in the perinuclear region.
IL 266:267 (≠ II 202:203) mutation to LA: Localizes in vesicular structures mainly concentrated in the perinuclear region.
SSLRN 268:272 (≠ ESHHQ 207:211) natural variant: Missing (in ISSD; alters intracellular localization, only partially targeted to lysosomes and mainly detected in LAMP1-negative vesicles and in the Golgi apparatus; abolishes H(+)-coupled sialic acid transporter activity; has normal L-aspartate and L-glutamate transporter activity)
G328 (≠ A266) to E: in ISSD; some patients may manifest a milder phenotype consistent with Salla disease; markedly decreases H(+)-coupled sialic acid transporter activity; abolishes L-aspartate and L-glutamate transporter activity; dbSNP:rs386833996
P334 (= P272) to R: in ISSD; does not affect intracellular localization, targeted to lysosomes; abolishes H(+)-coupled sialic acid transporter activity; abolishes L-aspartate and L-glutamate transporter activity; dbSNP:rs119491110
G371 (= G309) to V: in ISSD; abolishes H(+)-coupled sialic acid transporter activity; abolishes L-aspartate and L-glutamate transporter activity; dbSNP:rs777862172

Sites not aligning to the query:

22:23 Dileucine internalization motif; LL→AA: Targeted to plasma membrane.; LL→GG: Targeted to plasma membrane; sialic acid uptake strongly activated at acidic pH.
39 R → C: in SD; frequent variant in Finland; alters intracellular localization, only partially targeted to lysosomes and mainly detected in LAMP1-negative vesicles and in the Golgi apparatus; completely devoid of L-aspartate and L-glutamate transport activity, but retains appreciable H(+)-coupled sialic acid and nitrate transporter activity; dbSNP:rs80338794

Q8BN82 Sialin; H(+)/nitrate cotransporter; H(+)/sialic acid cotransporter; AST; Solute carrier family 17 member 5; Vesicular excitatory amino acid transporter; VEAT from Mus musculus (Mouse) (see paper)
23% identity, 79% coverage: 25:367/433 of query aligns to 80:440/495 of Q8BN82

query
sites
Q8BN82

T

S

R

K

N

E

T

C

V

A

A

E

A

H

A

S

A

A

P

P

T

I

L

K

M

V

E

H

L

N

H

H

I

T

-

G

-

K

-

Y

-

K

-

W

S

D

T

A

Q

E

Q

T

Y

Q

S

G

Y

W

I

I

I

L

A

G

A

S

Y

F

S

F

A

Y

A

G

Y

Y

T

I

V

V

M

T

Q

Q

P

I

V

P

A

G

G

G

Y

Y

V

I

L

A

D

S

V

R

L

V

G

G

T

G

K

K

I

L

G

L

Y

L

A

G

F

L

F

G

A

I

I

L

A

G

W

T

A

S

V

V

F

F

C

T

G

L

S

F

T

T

A

P

L

L

A

A

G

A

S

D

W

L

G

G

-

V

G

T

L

L

A

V

L

V

A

L

R

R

G

A

A

L

V

E

G

G

A

L

A

G

E

E

A

G

A

V

M

T

I

F

P

P

A

A

G

M

L
x
H

K

A

A

M

S

W

S

S

E

S

W

W

F

A

P

P

A

P

K

L

E

E

R

R

S

S

I

K

A

L

V

L

G

T

Y

I

F

S

N

Y

V

A

G

G

S

A

S

Q

I

L

G

G

A

T

M

V

I

I

A

S

P

L

P

P

L

L

V

S

V

G

W

I

A

I

I

C

V

Y

M

Y

H

M

S

N

W

W

Q

T

M

Y

A

V

F

F

I

Y

I

L

S

F

G

G

V

I

L

V

S

G

F

I

A

V

W

W

A

F

M

I

A

L

W

W

L

M

-

W

V

I

F

V

Y

S

K

D

H

T

P

P

R

E

D

T

Q

H

K

K

K

T

L

I

T

S

E

H

E

Y

E

E

R

K

N

E

Y

Y

I

I

I

V

G

S

Q

L

E

K

S

N

H

Q

H

L

Q

S

T

S

N

-

G

-

K

Q

K

K

M

V

S

V

P

P

W

W

-

G

Q

S

I

I

L

L

R

K

N

S

R

L

Q

P

F

L

W

W

G

A

I

I

A

V

L

V

P

A

R

H

F

F

L

S

A

Y

E

N

P

W

A

S

W

F

G

Y

T

T

F

L

N

L

A

T

W

L

I

L

P

P

L

T

F

Y

M

M

F

K

K

E

V

I

Y

L

G

R

F

F

N

N

L

V

K

Q

E

E

I

N

A

G

M

F

F

L

A

S

W

A

M

L

P

P

M

Y

L

F

F

G

A

C

D

W

L

L

G

C

C

M

I

I

V

L

G

C

G

G

Y

Q

L

A

P

A

P

D

L

Y

F

L

Q

R

R

V

W

K

F

W

G

N

V

F

N

S

L

T

I

I

V

S

S

V

R

R

K

R

M

I

V

F

V

S

T

L

M

V

G

G

-

M

-

V

-

G

-

P

A

A

L

V

L

F

M

L

I

V

G

A

P

A

G

G

M

F

I

I

G

G

L

C

F

D

T

Y

S

S

P

-

F

-

V

L

A

A

I

V

A

A

L

F

L

L

C

T

V

I

-

S

-

T

-

L

G

G

F

F

A

A

H

S

Q

S

A

G

L

F

S

S

G

I

A

N

L

H

I

L

T

D

L

I

S

A

S

P

D

S

V

Y

F

A

G

G

-

I

-

L

-

G

-

I

R

T

N

N

E

T

V

F

A

A

T

T

A

I

N

P

G

G

M

M

T

T

G

G

H183 (≠ L120) mutation to R: Abolishes sialic acid transporter activity. Does not affect L-aspartate and L-glutamate transporter activity.

Sites not aligning to the query:

39 R→C: Completely abolishes L-aspartate and L-glutamate transporter activity. Retains appreciable H(+)-coupled sialic acid transporter activity.

Q5Q0U0 Sialin; H(+)/nitrate cotransporter; H(+)/sialic acid cotransporter; AST; Solute carrier family 17 (Anion/sugar transporter), member 5; Vesicular excitatory amino acid transporter; VEAT from Rattus norvegicus (Rat) (see 2 papers)
24% identity, 82% coverage: 12:367/433 of query aligns to 62:440/495 of Q5Q0U0

query
sites
Q5Q0U0

I

V

A

A

L

L

V

V

T

D

L

M

G

V

T

D

V

S

L

N

G

S

Y

T

L

L

T

T

R

D

N

N

T

R

V

T

A

S

A

K

-

E

-

C

-

A

-

E

-

H

A

S

A

A

P

P

T

V

L

K

M

V

E

H

L

N

H

H

I

T

-

G

-

K

-

Y

-

Q

-

W

S

D

T

A

Q

E

Q

T

Y

Q

S

G

Y

W

I

I

I

L

A

G

A

S

Y

F

S

F

A

Y

A

G

Y

Y

T

I

V

V

M

T

Q

Q

P

I

V

P

A

G

G

G

Y

Y

V

M

L

A

D

S

V

R

L

I

G

G

T

G

K
|
K

I

L

G

L

Y

L

A

G

F

L

F

G

A

I

I

L

A

G

W

T

A

A

V

V

F

F

C

T

G

L

S

F

T

T

A

P

L

L

A

A

G

A

S

D

W

L

G

G

G

V

L

V

A

A

L

L

A

I

-

V

-

L

R
|
R

G

A

A

L

V
x
E

G
|
G

A

L

A

G

E
|
E

A
x
G

A

V

M

T

I
x
F

P
|
P

A

A

G

M

L
x
H

K

A

A

M

S
x
W

S

S

E

S

W

W

F

A

P

P

A

P

K

L

E

E

R

R

S

S

I

K

A

L

V

L

G

T

Y

I

F

S

N

Y

V

A

G

G

S

A

S

Q

I

L

G

G

A

T

M

V

I

I

A

S

P

L

P

P

L

L

V

S

V

G

W

I

A

I

I

C

V

Y

M

Y

H

M

S

N

W

W

Q

T

M

Y

A

V

F

F

I

Y

I

L

S

F

G

G

V

L

L

V

S

G

F

I

A

V

W

W

A

F

M

I

A

L

W

W

L

M

-

W

V

I

F

V

Y

S

K

D

H

T

P

P

R

E

D

T

Q

H

K

K

K

T

L

I

T

S

E

H

E

Y

E

E

R

K

N

E

Y

Y

I

I

I

V

G
x
S

Q
x
L

E
x
K

S
x
N

H

Q

H

L

Q

S

T

S

N

-

G

-

K

Q

K

K

M

V

S

V

P

P

W

W

-

V

Q

S

I

I

L

L

R

K

N

S

R

L

Q

P

F

L

W

W

G

A

I

I

A

V

L

V

P

A

R

H

F

F

L

S

A

Y

E

N

P

W

A

T

W

F

G

Y

T

T

F

L

N

L

A

T

W

L

I

L

P

P

L

T

F

Y

M

M

F

K

K

E

V

I

Y

L

G

R

F

F

N

N

L

V

K

Q

E

E

I

N

A

G

M

F

F

L

A

S

W

A

M

L

P
|
P

M

Y

L

F

F

G

A

C

D

W

L

L

G

C

C

M

I

I

V

V

G

C

G

G

Y

Q

L

A

P

A

P

D

L

Y

F

L

Q

R

R

V

W

K

F

W

G

N

V

F

N

S

L

T

I

I

V

S

S

V

R

R

K

R

M

I

V

F

V

S

T

L

M

I

G
|
G

-

M

-

V

-

G

-

P

A

A

L

V

L

F

M

L

I

V

G

A

P

A

G

G

M

F

I

I

G

G

L

C

F

D

T

Y

S

S

P

-

F

-

V

L

A

A

I

V

A

A

L

F

L

L

C

T

V

I

-

S

-

T

-

L

G

G

F

F

A

A

H

S

Q

S

A

G

L

F

S

S

G

I

A

N

L

H

I

L

T

D

L

I

S

A

S

P

D

S

V

Y

F

A

G

G

-

I

-

L

-

G

-

I

R

T

N

N

E

T

V

F

A

A

T

T

A

I

N

P

G

G

M

M

T

I

G

G

K136 (= K75) mutation to E: Markedly decreases H(+)-coupled sialic acid transporter activity.
R168 (= R105) mutation to C: Abolishes H(+)-coupled sialic acid transporter activity.
E171 (≠ V108) mutation to C: Decreases H(+)-coupled sialic acid transporter activity; when associated with C-175.
G172 (= G109) mutation to C: Decreases protein levels and alters subcellular localization.
E175 (= E112) mutation to C: Decreases H(+)-coupled sialic acid transporter activity; when associated with C-171.
G176 (≠ A113) mutation to C: Decreases protein levels and alters subcellular localization.
F179 (≠ I116) mutation to C: Decreases the affinity and transport rate for D-glucuronate. Does not affect H(+)-coupled sialic acid transporter activity.
P180 (= P117) mutation to C: Decreases protein levels and alters subcellular localization.
H183 (≠ L120) mutation to R: Abolishes H(+)-coupled sialic acid transporter activity.
W186 (≠ S123) mutation to C: Abolishes H(+)-coupled sialic acid transporter activity.
SSLKN 268:272 (≠ GGQES 204:208) mutation Missing: Abolishes H(+)-coupled sialic acid transporter activity.
P334 (= P272) mutation to R: Abolishes H(+)-coupled sialic acid transporter activity.
G371 (= G309) mutation to V: Remains in the endoplasmic reticulum.

Sites not aligning to the query:

22:23 Dileucine internalization motif; LL→AA: Targeted to plasma membrane; sialic acid uptake strongly activated at acidic pH.
39 R→C: Markedly decreases H(+)-coupled sialic acid transporter activity.

Q03567 Uncharacterized transporter slc-17.2 from Caenorhabditis elegans (see paper)
27% identity, 54% coverage: 101:333/433 of query aligns to 139:381/493 of Q03567

query
sites
Q03567

L

L

A

A

L

I

A

L

R

R

G

A

A

A

V

I

G

G

A

F

A

L

E

Q

A

G

A

A

M

T

I

F

P

P

A

A

G

M

L

H

K

T

A

M

S

W

S

S

E

V

W

W

F

G

P

P

A

P

K

L

E

E

R

L

S

S

I

V

A

L

V

T

G

G

Y

V

F

T

N

Y

V

A

G

G

S

A

S

Q

I

I

G

G

A

N

M

V

I

I

A

V

P

L

P

P

L

L

V

S

V

G

W

F

A

L

I

C

V

E

M

Y

H

G

-

F

-

D

-

G

S

G

W

W

Q

P

M

S

A

I

F

F

I

Y

I

I

S

I

G

G

V

V

L

F

S

G

F

V

A

L

W

W

-

T

A

A

M

V

A

W

W

W

L

Y

V

V

F

S

Y

S

K

D

H

K

P

P

R

A

D

T

Q

H

K

P

K

R

L

I

T

T

E

P

E

E

R

K

N

Q

Y

Y

I

I

I

V

G

T

G

A

Q

V

E

E

S

A

H

S

H

M

Q

G

T

K

N

D

N

T

G

G

K

K

K

V

M

P

S

S

-

T

P

P

W

W

-

I

Q

K

I

I

L

L

R

T

N

S

R

P

Q

A

F

V

W

W

G

A

I

C

A

W

L

A

P

G

R

H

F

F

L

A

A

G

E

D

P

-

A

-

W

W

G

G

T

A

F

Y

N

T

A

M

W

L

I

V

-

S

-

L

P

P

L

S

F

F

M

L

F

K

K

D

V

V

Y

L

G

G

F

L

N

N

L

L

K

S

E

S

I

L

A

G

M

A

F

V

A

A

W

S

M

I

P

P

M

Y

L

I

F

A

A

Y

D

F

L

L

G

A

C

I

I

N

V

A

G

G

Y

V

L

L

P

A

P

D

L

T

F

L

Q

R

R

S

W

K

F

G

G

I

V

L

N

S

L

T

I

L

V

N

S

T

R

R

K

R

-

A

-

M

M

L

V

V

V

A

T

L

M

I

G

G

-

Q

A

G

L

I

L

F

M

L

I

V

G

A

P

S

G

G

M

Y

I

C

G

G

L

C

F

G

T

Q

S

D

P

V

F

L

V

V

A

I

I

I

A

F

L

I

L

T

C

C

Sites not aligning to the query:

69 modified: carbohydrate, N-linked (GlcNAc...) asparagine

Q9JI12 Vesicular glutamate transporter 2; VGluT2; Differentiation-associated BNPI; Differentiation-associated Na(+)-dependent inorganic phosphate cotransporter; Solute carrier family 17 member 6 from Rattus norvegicus (Rat) (see 2 papers)
24% identity, 76% coverage: 98:428/433 of query aligns to 177:513/582 of Q9JI12

query
sites
Q9JI12

W

Y

G

G

G

C

L

V

A

I

L

F

A

V

R
|
R

G

I

A

L

V

Q

G

G

A

L

A

V

E
|
E

A

G

A

V

M

T

I

Y

P

P

A

A

G

C

L

H

K

G

A

I

S

W

S

S

E

K

W

W

F

A

P

P

A

P

K

L

E

E

R

R

S

S

I

R

A

L

V

A

G

T

Y

T

F

S

N

F

V

C

G

G

S

S

S

Y

I

A

G

G

A

A

M

V

I

I

A

A

P

M

P

P

L

L

V

A

V

G

W

I

A

L

I

V

V

Q

M

Y

H

T

S

G

W

W

Q

S

M

S

A

V

F

F

I

Y

I

V

S

Y

G

G

V

S

L

F

S

G

F

M

A

V

W

W

A

Y

M

M

A

F

W

W

-

L

L

L

V

V

F

S

Y

Y

K

E

H

S

P

P

R

A

D

K

Q

H

K

P

K

T

L

I

T

T

E

D

E

E

R

R

N

R

Y

Y

I

I

I

-

G

-

Q

E

E

E

S

S

H

I

H

G

Q

E

T

S

N

A

N

N

-

L

-

G

-

A

-

M

G

E

K

K

K

F

M

K

S

T

P

P

W

W

-

R

Q

K

I

F

L

F

R

T

N

S

R

M

Q

P

F

V

W

Y

G

A

I

I

A

I

L

V

P

A

R

N

F

F

L

C

A
x
R

E

-

P

-

A

S

W

W

G

T

T

F

F

Y

N

L

A

L

W

L

I

I

-

S

-

Q

P

P

L

A

F

Y

M

F

F

E

K

E

V

V

Y

F

G

G

F

F

N

E

L

I

K

S

E

K

I

V

A

G

M

M

F

L

A

S

W

A

M

V

P

P

M

H

L

L

F

V

A

M

D

T

L

I

G

I

C

V

I

P

V

I

G

G

Y

Q

L

I

P

A

P

D

L

F

F

L

Q

R

R

S

W

K

F

Q

G

I

V

L

N

S

L

T

I

T

V

T

S

V

R

R

K

K

M

I

V

M

V

N

T

C

-

G

-

F

-

G

M

M

G

E

A

A

L

T

L

L

M

L

I

L

G

-

P

-

G

-

M

V

I

V

G

G

L

Y

F

S

T

H

S

T

P

R

F

G

V

V

A

A

I

I

A

S

L

F

L

L

C

V

V

L

G

A

-

V

G

G

F

F

A

S

H

G

Q

F

A

A

L

I

S

S

G

G

A

F

L

N

I

V

T

N

L

-

S

H

S

L

D

D

V

I

F

A

G

P

R

R

N

Y

E

-

V

A

A

S

T

I

A

L

N

M

G

G

M

I

T

S

G

N

M

G

A

V

A

G

W

T

L

L

A

S

S

G

T

M

M

V

F

C

A

P

L

I

V

I

V

V

G

G

A

A

L

M

A

T

D

K

T

N

I

K

G

S

F

R

S

E

P

E

-

W

-

Q

-

Y

L

V

F

F

A

L

V

I

L

A

A

A

V

L

F

V

D

H

V

-

L

Y

G

G

A

G

V

V

V

I

I

F

W

Y

T

A

V

L

L

F

K

A

N

S

K

G

S

E

A

K

D

Q

D

P

D

W

S

A

T

D

P

P

L

E

E

E

T

T

S

S

K

E

R184 (= R105) mutation R->A,E,K: Greatly lowers L-glutamate transport.
E191 (= E112) mutation to A: Greatly lowers L-glutamate transport.; mutation E->D,Q: Lowers L-glutamate transport.
R322 (≠ A239) mutation to A: Loss of L-glutamate release. Abolishes the chloride ion conductance.

Sites not aligning to the query:

88 R→A: Impairs synaptic transmission. Abolishes the chloride ion conductance.
128 H→A: Greatly lowers L-glutamate transport.

7t3nA R184q/e191q mutant of rat vesicular glutamate transporter 2 (vglut2)
27% identity, 55% coverage: 109:344/433 of query aligns to 130:371/452 of 7t3nA

query
sites
7t3nA

G

G

A

L

A

V

E

Q

A

G

A

V

M

T

I
x
Y

P

P

A

A

G

C

L

H

K

G

A

I

S

W

S

S

E

K

W

W

F

A

P

P

A

P

K

L

E
|
E

R

R

S

S

I

R

A

L

V

A

G

T

Y

T

F

S

N

F

V

C

G
|
G

S

S

S
x
Y

I

A

G

G

A

A

M

V

I

I

A

A

P

M

P

P

L

L

V

A

V

G

W

I

A

L

I

V

V

Q

M

Y

H

T

S

G

W

W

Q

S

M

S

A

V

F

F

I

Y

I

V

S

Y

G

G

V

S

L

F

S

G

F

M

A

V

W

W

A

Y

M

M

A

F

W

W

-

L

L

L

V

V

F

S

Y

Y

K

E

H

S

P

P

R

A

D

K

Q

H

K

P

K

T

L

I

T

T

E

D

E

E

R

R

N

R

Y

Y

I

I

I

-

G

-

Q

E

E

E

S

S

H

I

H

G

Q

E

T

S

N

A

N

N

-

L

-

G

-

A

-

M

G

E

K

K

K

F

M

K

S

T

P

P

W

W

-

R

Q

K

I

F

L

F

R

T

N

S

R

M

Q

P

F

V

W

Y

G

A

I

I

A

I

L

V

P

A

R

N

F

F

L

C

A
x
R

E

-

P

-

A

S

W

W

G

T

T
x
F

F
x
Y

N

L

A

L

W

L

I

I

-

S

-

Q

P

P

L

A

F

Y

M

F

F

E

K

E

V

V

Y

F

G

G

F

F

N

E

L

I

K

S

E

K

I

V

A

G

M

M

F

L

A

S

W

A

M

V

P

P

M

H

L

L

F

V

A

M

D

T

L

I

G

I

C

V

I

P

V

I

G

G

Y

Q

L

I

P

A

P

D

L

F

F

L

Q

R

R

S

W

K

F

Q

G

I

V

L

N

S

L

T

I

T

V

T

S

V

R

R

K

K

M

I

V

M

V

N

T

C

-

G

-

F

-

G

M

M

G

E

A

A

L

T

L

L

M

L

I

L

G

-

P

-

G

-

M

V

I

V

G

G

L

Y

F

S

T

H

S

T

P

R

F

G

V

V

A

A

I

I

A

S

L

F

L

L

C

V

V

L

G

A

-

V

G

G

F

F

A

S

H

G

Q

F

A

A

L

I

S

S

G

G

binding (1s,3r)-1-aminocyclopentane-1,3-dicarboxylic acid: Y137 (≠ I116), Y165 (≠ S144), R264 (≠ A239), F268 (≠ T245), Y269 (≠ F246)
binding (2R)-2-(methoxymethyl)-4-{[(25R)-spirost-5-en-3beta-yl]oxy}butyl 4-O-alpha-D-glucopyranosyl-beta-D-glucopyranoside: E152 (= E131), G163 (= G142)

Sites not aligning to the query:

P53322 High-affinity nicotinic acid transporter; Nicotinic acid permease from Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast) (see paper)
20% identity, 55% coverage: 2:237/433 of query aligns to 83:327/534 of P53322

query
sites
P53322

R

R

K

V

I

L

K

R

G

K

L

M

R

D

W

I

Y

Y

M

L

I

I

A

P

L

L

V

M

T

G

L

M

G

L

T

Y

V

F

L

L

G

S

Y

N

L

L

T

D

R

K

N

S

T

N

V

I

A

G

-

N

A

A

A

E

A

V

P

A

T

G

L

L

M

S

E

K

E

D

L

I

H

H

I

L

S

V

T

G

Q

T

Q

Q

Y

Y

S

N

Y

T

I

C

I

V

A

T

A

V

Y

F

S

F

A

A

A

T

Y

Y

T

V

V

L

M

F

Q

D

P

P

V

I

A

G

G

T

Y

N

V

L

L

L

D

K

V

I

L

M

G

G

T

P

K

P

I

L

G

M

Y

M

A

S

F

I

F

C

A

L

I

T

A

C

W

F

A

G

V

A

F

I

C

S

G

L

S

G

T

T

A

A

L

W

A

V

G

K

S

N

W

Y

G

A

G

Q

L

L

A

I

L

V

A

V

R

R

G

L

A

L

V

L

G

G

A

A

A

F

E

E

A

G

A

M

M

I

I

Y

P

P

A

A

G

I

L

N

K

M

A

Y

S

L

S

S

E

V

W

C

F

Y

P

R

A

R

K

E

E

Q

R

Y

S

A

I

L

A

R

V

F

G

A

Y

F

F

V

-

F

-

S

-

A

N

C

V

L

G

S

S

S

I

F

G

G

A

G

M

L

I

I

A

A

P

Y

P

G

L

C

V

S

V

K

W

I

A

S

I

G

V

S

M

L

H

K

S

D

W

W

Q

Q

M

Y

A

I

F

Y

I

I

I

V

S

E

G

G

V

C

L

I

S

S

F

-

A

-

W

-

A

-

M

L

A

G

W

F

L

V

V

P

F

F

Y

Y

-

A

-

F

-

G

-

L

-

S

K

K

H

N

P

L

R

E

D

D

Q

S

K

W

K

F

L

F

T

N

E
x
K

E

E

R

K

N

E

Y

Y

I

I

I

S

G

E

G

R

Q

Y

E

K

S

T

H

M

H

N

Q

T

T

F

N

D

N

P

G

D

K

E

K

K

M

F

S

E

-

W

-

F

-

Q

P

V

W

W

Q

Q

I

A

L

V

R

K

N

D

R

V

Q

K

F

T

W

W

G

A

I

S

A

A

L

V

P

A

R

L

F

F

K283 (≠ E196) modified: Glycyl lysine isopeptide (Lys-Gly) (interchain with G-Cter in ubiquitin)

Q9C0U9 Uncharacterized transporter PB1C11.03 from Schizosaccharomyces pombe (strain 972 / ATCC 24843) (Fission yeast) (see paper)
25% identity, 45% coverage: 36:229/433 of query aligns to 128:321/570 of Q9C0U9

query
sites
Q9C0U9

L

L

M

F

E

D

L

V

H

H

I

I

S

T

T

S

Q

N

Q

Q

Y

Y

S

N

Y

N

I

L

I

N

A

T

A

L

Y

F

S

Y

A

V

A

G

Y

Y

T

I

V

V

M

G

Q

Q

P

F

V

P

A

G

G

H

Y

Y

V

I

L

M

D

Q

V

T

-

F

-

P

L

L

G

G

T

K

K

F

I

V

G

G

Y

L

A

V

F

T

F

F

A

-

I

-

A

S

W

W

A

S

V

V

F

I

C

V

G

F

S

L

T

H

A

C

L

C

A

A

G

Y

S

N

W

Y

G

G

L

L

A

I

L

A

A

L

R

R

G

F

A

F

V

L

G

G

A

F

A

T

E

E

A

S

A

C

M

L

I

L

P

P

A

A

G

M

L

E

K

A

A

T

S

M

S

G

E

M

W

F

F

F

P

T

A

H

K

Q

E

E

R

Q

S

A

I

F

A

L

V

Q

G

P

Y

V

F

F

N

W

V

I

G

S

S

C

-

L

S

S

I

C

G

G

-

I

-

P

-

A

A

G

M

F

I

I

A

A

P

Y

P

G

L

L

V

E

V

F

W

V

A

T

I

K

V

S

M

I

H

A

S

P

W

W

Q

K

M

L

A

F

F

M

I

I

S

T

G

G

V

G

L

I

S

T

F

F

A

F

W

L

A

S

M

I

A

F

W

L

L

F

V

F

F

Y

Y

Y

-

P

K

D

H

N

P

P

R

S

D

K

Q

A

K

R

K

F

L

L

T

T

E

D

E

E

R

K

N

L

Y

Y

I

T

I

I

G

-

Q

D

E

R

S

V

H

R

H

K

Q

S

T

T

N

R

N

G

G

G

K

I

K

E

M

N

S

-

P

-

W

-

Q

K

I

I

L

F

R

K

N

K

R

H

Q

Q

F

F

Sites not aligning to the query:

14 modified: Phosphoserine

Q9Y2C5 Probable small intestine urate exporter; Solute carrier family 17 member 4 from Homo sapiens (Human) (see 2 papers)
21% identity, 57% coverage: 65:312/433 of query aligns to 128:377/497 of Q9Y2C5

query
sites
Q9Y2C5

A

S

G

G

Y

Y

V

V

L

A

D

G

V

I

L

F

G

G

T

A

K

K

-

Y

-

V

I

V

G

G

Y

A

A

G

F

L

F

F

A

I

I

S

A

S

W

F

A

L

V

T

F

L

C

F

G

I

S

P

T

L

A

A

L

A

A

N

G

A

S

G

W

V

G

A

G

L

L

L

A

I

L

V

A

L

R

R

G

I

A

V

V

Q

G

G

A

I

A

A

E

Q

A

V

A

M

M

V

I

L

P

T

A

G

G

Q

L

Y

K

S

A

I

S

W

S

V

E

K

W

W

F

A

P

P

A

P

K

L

E

E

R

R

S

S

I

Q

A

L

V

T

G

T

Y

I

F

A

N

G

V

S

G

G

S

S

S

M

I

L

G

G

A

S

M

F

I

I

A

V

P

-

L

L

V

L

V

A

W

G

A

G

I

L

V

L

M

C

H

Q

S

T

-

I

-

G

W

W

Q

P

M

Y

A

V

F

F

I

Y

I

I

S

F

G

G

V

G

L

I

S

G

F

C

A

A

W

C

A

C

M

P

A

L

W

W

L

F

-

P

V

L

F

I

Y

Y

K

D

H

D

P

P

R

V

D

N

Q

H

K

P

K

F

L

I

T

S

E

A

E

G

E

E

R

K

N

R

Y

Y

I

I

I

V

G

C

G

S

Q

L

E

-

S

A

H

Q

Q

D

T

C

N

S

N

P

G

G

K

W

K

S

M

L

S

P

P

I

W

R

Q

A

I

M

L

I

R

K

N

S

R

L

Q

P

F

L

W

W

G

A

I

I

A

L

L

V

P

S

R

Y

F

F

L

C

A

E

E

Y

P

W

A

L

W

F

G

Y

T

T

F

I

N

M

A

A

W

Y

I

T

P

P

L

T

F

Y

M

I

F

S

K

S

V

V

Y

L

G

Q

F

A

N

N

L

L

K

R

E

D

I

S

A

G

M

I

F

L

A

S

W

A

M

L

P

P

M

F

L

V

F

V

A

G

D

C

L

I

G

C

C

I

I

I

V

L

G

G

Y

L

L

L

P

A

P

D

L

F

F

L

Q

L

R

S

W

R

F

K

G

I

V

L

N

R

L

L

I

I

V

T

S

I

R

R

K

K

M

L

V

F

V

T

T
x
A

M

I

G

G

A

V

L

L

L

F

A372 (≠ T307) to T: in dbSNP:rs11754288

Sites not aligning to the query:

66 modified: carbohydrate, N-linked (GlcNAc...) asparagine; N→A: Loss of glycosylation and significant reduction in the transport of thyroid hormones T3 and T4; when associated with A-75 and A-90.
75 modified: carbohydrate, N-linked (GlcNAc...) asparagine; N→A: Loss of glycosylation and significant reduction in the transport of thyroid hormones T3 and T4; when associated with A-66 and A-90.
90 modified: carbohydrate, N-linked (GlcNAc...) asparagine; N→A: Loss of glycosylation and significant reduction in the transport of thyroid hormones T3 and T4; when associated with A-75 and A-90.

Q8IVW8 Sphingosine-1-phosphate transporter SPNS2; Protein spinster homolog 2 from Homo sapiens (Human) (see 2 papers)
23% identity, 56% coverage: 13:256/433 of query aligns to 106:342/549 of Q8IVW8

query
sites
Q8IVW8

A

A

L

I

V

L

T

S

L

L

G

G

T

N

V

V

L

L

G

N

Y

Y

L

L

T

D

R

R

N

Y

T

T

V

V

A

A

A

G

A

V

A

L

P

L

T

D

L

I

M

Q

E

Q

E

H

L

F

H

G

I

V

S

K

T

D

Q

R

Q

G

Y

A

S

G

Y

L

I

L

I

Q

A

S

A

V

Y

F

S

I

A

C

A

S

Y

F

T

M

V

V

M

A

Q

A

P

P

V

I

A

F

G

G

Y

Y

V

L

L

G

D

D

V

R

L

F

G

N

T

R

K

K

I

V

G

I

Y

L

A

S

F

C

F

G

A

I

I

F

A

F

W

W

A

S

-

A

-

V

V

T

F

F

C

S

G

S

S

S

T

F

A

I

L

P

A

Q

G

Q

S

Y

W

F

G

W

G

L

L

L

A

V

L

L

A

S

R
|
R

G

G

A

L

V

V

G

G

A

I

A

G

E

E

A

A

A

S

M

Y

I

S

P

T

A

I

G

A

L

P

K

T

A

I

S

I

S

G

E

D

W

L

F

F

P

T

A

K

K

N

E

T

R

R

S

T

-

L

-

M

I

L

A

S

V

V

G

F

Y

Y

F

F

N

A

V

I

-

P

-

L

G

G

S

S

S

G

I

L

G

G

A

Y

M

I

I

T

A

G

P

S

L

V

V

K

V

Q

W

A

A

A

I

-

V

-

M

-

H

G

S

D

W

W

Q

H

M

W

A

A

F

L

I

R

I

V

S

S

G

P

V

V

L

L

S

G

F

M

A

I

W

T

A

G

M

T

A

L

W

I

L

L

V

I

F

L

Y

V

K

-

H

-

P

P

R

A

D

T

Q

K

K

R

K

G

L

H

T

A

E

D

E

Q

E

L

R

-

N

-

Y

-

I

-

G

-

G

G

Q

D

E

Q

S

L

H

K

H

A

Q

R

T

T

N

S

N

W

G

L

K

R

K

D

M

M

S

K

P

-

W

-

Q

A

I

L

L

I

R

R

N

N

R

R

Q

S

F

Y

W

V

G

F

I

S

A
x
S

L

L

P

A

R

T

F

S

L

A

A

V

E

S

P

F

A

A

W

T

G

G

T

A

F

L

N

G

A

M

W

W

I

I

P

P

L

L

F

Y

M

L

F

H

K

R

R200 (= R105) mutation to S: Loss of function; does not rescue the cardia bifida phenotype in the morpholino knockdown in zebrafish.
S319 (≠ A233) natural variant: Missing (in DFNB115; uncertain significance; dbSNP:rs749994718)

P34711 Vesicular acetylcholine transporter unc-17; Uncoordinated protein 17 from Caenorhabditis elegans (see paper)
23% identity, 94% coverage: 23:430/433 of query aligns to 84:475/532 of P34711

query
sites
P34711

Y

Y

L

L

T

V

R

R

N

E

T

-

V

V

A

G

A

R

A

I

P

N

T

F

L

L

M

D

E

E

L

L

H

E

I

L

S

G

T

-

Q

-

Y

-

S

-

Y

W

I

L

I

F

A

A

A

S

Y

K

S

A

A

L

Y

Q

T

I

V

F

M

V

Q

N

P

P

V

F

A

S

G

G

Y

Y

V

I

L

I

D

D

V

R

L

V

G

G

T

Y

K

E

I

I

-

P

-

M

-

I

-

L

G

G

Y

L

A

C

F

T

F

M

A

F

I

F

A

S

W

T

A

A

V

I

F

F

C

-

G

-

S

-

T

-

A

A

L

L

A

G

G

K

S

S

W

Y

G

G

G

V

L

L

A

L

L

F

A

A

R

R

G

S

A

L

V

Q

G

G

A

F

A

G

E

S

A

A

A

F

M

A

I

D

P

T

A

S

G

G

L

L

K

A

A

M

S

I

S

A

E

D

W

R

F

F

P

T

A

E

K

E

-

N

E

E

R

R

S

S

I

A

A

A

V

L

G

G

Y

I

F

A

N

L

V

A

G

F

S

I

S

S

I

F

G

G

A

C

M

L

I

V

A

A

P

P

L

F

V

G

V

S

W

V

A

L

I

Y

V

S

M

L

H

A

S

G

W

K

Q

P

M

V

A

P

F

F

I

L

I

I

S

L

G

S

V

F

L

V

S
x
C

F

L

A

A

W

D

A

A

M

I

A

A

-

V

W

F

L

M

V

V

F

I

Y

N

K

P

H

H

P

R

R

R

D

-

Q

-

K

-

L

-

T

-

E

-

R

-

N

-

Y

-

I

-

G

-

G

G

Q

T

E

D

S

S

H

H

H

G

Q

E

T

K

N

V

N

Q

G

G

K

T

K

P

M

M

S

-

P

-

W

-

Q

-

I

-

L

-

R

-

N

-

R

-

Q

-

F

-

W

W

G

R

I

L

A

F

L

M

P

D

R

P

F

F

L

I

A

A

E

C

P

C

A

S

W

G

G

A

T

L

F

I

N

M

A

A

W

N

I

V

P

S

L

L

F

A

M

F

F

L

K

-

V

-

Y

-

G

-

F

-

N

-

L

-

K

-

E

E

I

P

A

T

M

I

F

T

A

T

W

W

M

M

P

S

M

E

L

M

F

M

A

P

D

D

L

T

-

P

G

G

C

W

I

L

V

V

G

G

G

V

-

I

Y

W

L

L

P

P

L

F

F

F

Q

P

R

H

W

V

F

L

G

G

V

V

N

Y

L

V

I

T

V

V

S

K

-

M

-

L

R

R

K

A

M

F

V

P

V

H

T

H

M

T

G

W

A

A

L

I

L

A

M

M

I

V

G

G

P

L

G

A

M

M

I

E

G
|
G

L

I

-

A

-

C

F

F

T

A

S

I

P

P

F

Y

-

T

-

S

-

V

-

M

-

Q

-

L

-

V

V

I

A

P

I

L

A

S

L

F

L

V

C

C

V

-

G

-

F

F

A

G

H

I

Q

A

A

L

L

I

S

D

G

T

A

S

L

L

I

L

T

P

L

M

S

L

S

G

D

H

V

L

F

V

G

D

R

T

N

R

E

H

V

V

A

S

T

V

A

Y

N

G

G

S

M

V

T

Y

G

A

M

I

A

A

A

D

W

I

L

S

A

Y

S

S

T

L

M

A

F

Y

A

A

-

F

-

G

-

P

L

I

V

I

V

A

G

G

A

W

L

I

A

V

D

T

T

N

I

W

G

G

F

F

S

T

P

A

L

L

F

N

A

I

V

I

L

I

A

F

V

A

F

T

D

N

V

V

L

T

G

Y

A

A

V

P

V

V

I

L

W

F

T

L

V

L

L

R

K

K

N

V

K

H

S

S

A

Y

D

D

D

T

-

L

-

G

-

A

-

K

-

G

-

D

-

T

-

A

D

E

S

M

T

T

P

Q

L

L

E

N

T

S

S

S

K

A

P

P

A

A

C230 (≠ S174) mutation to F: In p1160; severely uncoordinated.
G347 (= G320) mutation to A: In e245; severely uncoordinated.

8jhqA Cryo-em structure of human s1p transporter spns2 bound with s1p (see paper)
22% identity, 56% coverage: 13:256/433 of query aligns to 12:247/446 of 8jhqA

query
sites
8jhqA

A

A

L

I

V

L

T

S

L

L

G

G

T

N

V

V

L

L

G

N

Y

Y

L

L

T

D

R

R

N

Y

T

T

V

V

A

A

A

G

A

V

A

L

P

L

T

D

L

I

M

Q

E

Q

E

H

L

F

H

G

I

V

S

K

T

D

Q

R

Q

G

Y

A

S

G

Y

L

I

L

I

Q

A

S

A

V

Y

F

S

I

A

C

A

S

Y

F

T

M

V

V

M

A

Q

A

P

P

V

I

A

F

G

G

Y

Y

V

L

L

G

D

D

V

R

L

F

G

N

T

R

K

K

I

V

G

I

Y

L

A

S

F

C

F

G

A

I

I

F

A

F

W

W

A

S

-

A

-

V

V

T

F

F

C

S

G

S

S

S

T

F

A

I

L

P

A

Q

G

Q

S

Y

W

F

G

W

G

L

L

L

A

V

L

L

A

S

R

R

G

G

A

L

V

V

G

G

A

I

A

G

E

E

A

A

A

S

M

Y

I

S

P

T

A

I

G

A

L

P

K

T

A

I

S

I

S

G

E

D

W

L

F

F

P

K

-

N

A

T

K

R

E

T

R

L

S

M

I

L

A

S

V

V

G

F

Y

Y

F

F

N

A

V

I

-

P

-

L

G

G

S

S

S

G

I

L

G

G

A

Y

M

I

I

T

A

G

P

S

L

V

V

K

V

Q

W

A

A

A

I

-

V

-

M

-

H

G

S

D

W

W

Q

H

M

W

A

A

F

L

I

R

I

V

S

S

G

P

V

V

L

L

S

G

F

M

A

I

W

T

A

G

M

T

A

L

W

I

L

L

V

I

F

L

Y

V

K

-

H

-

P

P

R

A

D

T

Q

K

K

R

K

G

L

H

T

A

E

D

E

Q

E

L

R

-

N

-

Y

-

I

-

G

-

G

G

Q

D

E

Q

S

L

H

K

H

A

Q

R

T

T

N

S

N

W

G

L

K

R

K

D

M

M

S

K

P

A

W

-

Q

-

I

L

L

I

R

R

N

N

R

R

Q

S

F

Y

W

V

G

F

I

S

A

S

L

L

P

A

R
x
T

F

S

L

A

A

V

E

S

P

F

A

A

W

T

G

G

T

A

F
x
L

N

G

A

M

W

W

I

I

P

P

L

L

F

Y

M

L

F

H

K

R

binding (2S,3R,4E)-2-amino-3-hydroxyoctadec-4-en-1-yl dihydrogen phosphate: T227 (≠ R236), L237 (≠ F246)

Sites not aligning to the query:

binding (2S,3R,4E)-2-amino-3-hydroxyoctadec-4-en-1-yl dihydrogen phosphate: 338, 342, 368

Query Sequence

>BWI76_RS24735 FitnessBrowser__Koxy:BWI76_RS24735
MRKIKGLRWYMIALVTLGTVLGYLTRNTVAAAAPTLMEELHISTQQYSYIIAAYSAAYTV
MQPVAGYVLDVLGTKIGYAFFAIAWAVFCGSTALAGSWGGLALARGAVGAAEAAMIPAGL
KASSEWFPAKERSIAVGYFNVGSSIGAMIAPPLVVWAIVMHSWQMAFIISGVLSFAWAMA
WLVFYKHPRDQKKLTEEERNYIIGGQESHHQTNNGKKMSPWQILRNRQFWGIALPRFLAE
PAWGTFNAWIPLFMFKVYGFNLKEIAMFAWMPMLFADLGCIVGGYLPPLFQRWFGVNLIV
SRKMVVTMGALLMIGPGMIGLFTSPFVAIALLCVGGFAHQALSGALITLSSDVFGRNEVA
TANGMTGMAAWLASTMFALVVGALADTIGFSPLFAVLAVFDVLGAVVIWTVLKNKSADDD
STPLETSKPATQS

Or try a new SitesBLAST search

SitesBLAST's Database

SitesBLAST's database includes (1) SwissProt entries with experimentally-supported functional features; and (2) protein structures with bound ligands, from the BioLip database.

by Morgan Price, Arkin group
Lawrence Berkeley National Laboratory