SitesBLAST
Comparing BWI76_RS24855 FitnessBrowser__Koxy:BWI76_RS24855 to proteins with known functional sites using BLASTp with E ≤ 0.001.
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Found 20 (the maximum) hits to proteins with known functional sites (download)
P0AB74 D-tagatose-1,6-bisphosphate aldolase subunit KbaY; TBPA; TagBP aldolase; D-tagatose-bisphosphate aldolase class II; Ketose 1,6-bisphosphate aldolase class II; Tagatose-bisphosphate aldolase; EC 4.1.2.40 from Escherichia coli (strain K12) (see paper)
62% identity, 100% coverage: 1:284/284 of query aligns to 1:284/286 of P0AB74
- D82 (= D82) active site, Proton donor
- H83 (= H83) binding
- H180 (= H180) binding
- H208 (= H208) binding
1gvfB Structure of tagatose-1,6-bisphosphate aldolase (see paper)
60% identity, 99% coverage: 3:284/284 of query aligns to 2:275/275 of 1gvfB
- active site: D81 (= D82), H82 (= H83), H171 (= H180), H199 (= H208), N221 (= N230)
- binding phosphoglycolohydroxamic acid: D81 (= D82), H82 (= H83), H171 (= H180), G172 (= G181), H199 (= H208), G200 (= G209), S202 (= S211), N221 (= N230), V222 (= V231), A223 (= A232), T224 (= T233)
- binding zinc ion: H82 (= H83), H171 (= H180), H199 (= H208)
1rv8B Class ii fructose-1,6-bisphosphate aldolase from thermus aquaticus in complex with cobalt (see paper)
44% identity, 99% coverage: 2:283/284 of query aligns to 1:304/305 of 1rv8B
- active site: D80 (= D82), H81 (= H83), E140 (= E142), H178 (= H180), H208 (= H208), N251 (= N230)
- binding cobalt (ii) ion: H81 (= H83), E132 (= E134), H178 (= H180), H208 (= H208)
- binding sulfate ion: R116 (≠ K118), H123 (= H125), S211 (= S211), D253 (≠ A232), T254 (= T233)
P13243 Probable fructose-bisphosphate aldolase; FBP aldolase; FBPA; Fructose-1,6-bisphosphate aldolase; EC 4.1.2.13 from Bacillus subtilis (strain 168) (see paper)
44% identity, 95% coverage: 1:271/284 of query aligns to 1:272/285 of P13243
- T212 (≠ S211) modified: Phosphothreonine
- T234 (= T233) modified: Phosphothreonine
3q94A The crystal structure of fructose 1,6-bisphosphate aldolase from bacillus anthracis str. 'Ames ancestor'
42% identity, 100% coverage: 1:283/284 of query aligns to 1:284/285 of 3q94A
- active site: D85 (= D82), H86 (= H83), E145 (= E142), H181 (= H180), H209 (= H208), N231 (= N230)
- binding zinc ion: H86 (= H83), E114 (= E111), H163 (≠ E162), H181 (= H180), H209 (= H208), E235 (= E234), E239 (≠ A238)
3n9sA Class ii fructose-1,6-bisphosphate aldolase from helicobacter pylori in complex with n-(4-hydroxybutyl)- glycolohydroxamic acid bis- phosphate, a competitive inhibitor (see paper)
37% identity, 99% coverage: 3:284/284 of query aligns to 2:307/307 of 3n9sA
- active site: C69 (≠ A70), E70 (≠ K71), G136 (= G136), H180 (= H180), A226 (vs. gap), N253 (= N230)
- binding calcium ion: D104 (= D104), S106 (= S106), E134 (= E134)
- binding 4-{hydroxy[(phosphonooxy)acetyl]amino}butyl dihydrogen phosphate: N23 (= N24), S49 (≠ T50), D82 (= D82), H83 (= H83), H180 (= H180), G181 (= G181), K184 (vs. gap), H210 (= H208), G211 (= G209), S213 (= S211), N253 (= N230), D255 (≠ A232), T256 (= T233)
- binding zinc ion: H83 (= H83), H180 (= H180), H210 (= H208)
4to8A Methicillin-resistant staphylococcus aureus class iib fructose 1,6- bisphosphate aldolase (see paper)
42% identity, 98% coverage: 3:280/284 of query aligns to 2:273/279 of 4to8A
8q5aA Crystal structure of metal-dependent class ii sulfofructosephosphate aldolase from hafnia paralvei hpsqia-zn in complex with dihydroxyacetone phosphate (dhap) (see paper)
39% identity, 100% coverage: 1:283/284 of query aligns to 1:275/276 of 8q5aA
8q59A Crystal structure of metal-dependent class ii sulfofructose phosphate aldolase from yersinia aldovae in complex with sulfofructose phosphate (yasqia-zn-sfp) (see paper)
37% identity, 100% coverage: 1:283/284 of query aligns to 1:275/280 of 8q59A
- binding (3~{S},4~{S})-2,3,4-tris(oxidanyl)-5-oxidanylidene-6-phosphonooxy-hexane-1-sulfonic acid: G50 (≠ T50), Q51 (≠ P51), K52 (≠ G52), D82 (= D82), H83 (= H83), H172 (= H180), G173 (= G181), H200 (= H208), G201 (= G209), S203 (= S211), N222 (= N230), D224 (≠ A232), T225 (= T233)
- binding zinc ion: H83 (= H83), H172 (= H180), H200 (= H208)
A8B2U2 Fructose-bisphosphate aldolase; Glfba; glFBPA; Fructose-1,6-bisphosphate aldolase; EC 4.1.2.13 from Giardia intestinalis (strain ATCC 50803 / WB clone C6) (Giardia lamblia) (see 4 papers)
36% identity, 100% coverage: 1:283/284 of query aligns to 1:306/323 of A8B2U2
- S50 (≠ T50) binding
- D83 (= D82) active site, Proton donor; mutation to A: Severe loss of catalytic activity.
- H84 (= H83) binding
- H178 (= H180) binding ; binding
- G179 (= G181) binding
- K182 (≠ A184) binding
- H210 (= H208) binding
- G211 (= G209) binding
- S213 (= S211) binding
- N253 (= N230) binding
- D255 (≠ A232) binding ; mutation to A: 9.4-fold reduction in substrate affinity and 50-fold reduction in catalytic affinity. Has some activity towards tagatose-1,6-bisphosphate.
- S256 (≠ T233) binding
- R259 (≠ K236) binding ; mutation to A: 1.8-fold reduction in substrate affinity and 2.8-fold reduction in catalytic efficiency. 6-fold reduction in substrate affinity and 24-fold reduction in catalytic efficiency; when associated with A-278.
- D278 (= D255) mutation to A: 159-fold reduction in substrate affinity and 2770-fold reduction in catalytic efficiency. 6-fold reduction in substrate affinity and 24-fold reduction in catalytic efficiency; when associated with A-259.
- R280 (= R257) binding
3ohiA Structure of giardia fructose-1,6-biphosphate aldolase in complex with 3-hydroxy-2-pyridone (see paper)
36% identity, 98% coverage: 7:283/284 of query aligns to 6:302/319 of 3ohiA
- binding ({3-hydroxy-2-oxo-4-[2-(phosphonooxy)ethyl]pyridin-1(2H)-yl}methyl)phosphonic acid: S49 (≠ T50), D82 (= D82), H83 (= H83), H174 (= H180), G175 (= G181), K178 (≠ A184), G207 (= G209), S209 (= S211), N249 (= N230), D251 (≠ A232), S252 (≠ T233), R255 (≠ K236)
- binding zinc ion: H83 (= H83), H174 (= H180), H206 (= H208)
3gayA Structure of giardia fructose-1,6-biphosphate aldolase in complex with tagatose-1,6-biphosphate (see paper)
36% identity, 98% coverage: 7:283/284 of query aligns to 6:302/319 of 3gayA
- binding 1,6-di-O-phosphono-D-tagatose: N23 (= N24), S49 (≠ T50), D82 (= D82), H174 (= H180), G175 (= G181), K178 (≠ A184), H206 (= H208), G207 (= G209), S209 (= S211), N249 (= N230), D251 (≠ A232), S252 (≠ T233), R255 (≠ K236)
- binding zinc ion: H83 (= H83), H174 (= H180), H206 (= H208)
3n9rA Class ii fructose-1,6-bisphosphate aldolase from helicobacter pylori in complex with n-(4-hydroxybutyl)-phosphoglycolohydroxamic acid, a competitive inhibitor (see paper)
35% identity, 99% coverage: 3:284/284 of query aligns to 2:297/297 of 3n9rA
- active site: C69 (≠ A70), E70 (≠ K71), G136 (= G136), H170 (= H180), A216 (vs. gap), N243 (= N230)
- binding 2-[hydroxy(4-hydroxybutyl)amino]-2-oxoethyl dihydrogen phosphate: H83 (= H83), H170 (= H180), G171 (= G181), K174 (vs. gap), H200 (= H208), G201 (= G209), S203 (= S211), N243 (= N230), D245 (≠ A232), T246 (= T233)
- binding zinc ion: H83 (= H83), H170 (= H180), H200 (= H208)
3c56A Class ii fructose-1,6-bisphosphate aldolase from helicobacter pylori in complex with n-(3-hydroxypropyl)-glycolohydroxamic acid bisphosphate, a competitive inhibitor (see paper)
35% identity, 99% coverage: 3:284/284 of query aligns to 2:297/297 of 3c56A
- active site: C69 (≠ A70), E70 (≠ K71), G136 (= G136), H170 (= H180), A216 (vs. gap), N243 (= N230)
- binding 3-{hydroxy[(phosphonooxy)acetyl]amino}propyl dihydrogen phosphate: N23 (= N24), S49 (≠ T50), D82 (= D82), H170 (= H180), K174 (vs. gap), G201 (= G209), S203 (= S211), N243 (= N230), D245 (≠ A232), T246 (= T233), R249 (≠ K236)
- binding zinc ion: H83 (= H83), H170 (= H180), H200 (= H208)
3c52A Class ii fructose-1,6-bisphosphate aldolase from helicobacter pylori in complex with phosphoglycolohydroxamic acid, a competitive inhibitor (see paper)
35% identity, 99% coverage: 3:284/284 of query aligns to 2:296/296 of 3c52A
- active site: C69 (≠ A70), E70 (≠ K71), G136 (= G136), H169 (= H180), A215 (vs. gap), N242 (= N230)
- binding calcium ion: D104 (= D104), S106 (= S106), E134 (= E134)
- binding phosphoglycolohydroxamic acid: D82 (= D82), H83 (= H83), H169 (= H180), K173 (vs. gap), H199 (= H208), G200 (= G209), S202 (= S211), N242 (= N230), D244 (≠ A232), T245 (= T233)
- binding zinc ion: H83 (= H83), H169 (= H180), H199 (= H208)
3gb6A Structure of giardia fructose-1,6-biphosphate aldolase d83a mutant in complex with fructose-1,6-bisphosphate (see paper)
36% identity, 98% coverage: 7:283/284 of query aligns to 6:301/318 of 3gb6A
- binding 1,6-di-O-phosphono-D-fructose: N23 (= N24), S49 (≠ T50), H173 (= H180), G174 (= G181), K177 (≠ A184), H205 (= H208), G206 (= G209), S208 (= S211), N248 (= N230), D250 (≠ A232), S251 (≠ T233), R254 (≠ K236)
5uckA Class ii fructose-1,6-bisphosphate aldolase of helicobacter pylori with cleavage products (see paper)
34% identity, 99% coverage: 3:284/284 of query aligns to 2:291/291 of 5uckA
- binding glyceraldehyde-3-phosphate: S49 (≠ T50), D82 (= D82), H83 (= H83), H164 (= H180), D239 (≠ A232), R243 (≠ K236)
- binding zinc ion: H83 (= H83), H83 (= H83), E134 (= E134), H164 (= H180), H194 (= H208), H194 (= H208)
5ucpA Class ii fructose-1,6-bisphosphate aldolase e142a variant of helicobacter pylori with fbp and cleavage products (see paper)
34% identity, 99% coverage: 3:284/284 of query aligns to 2:292/292 of 5ucpA
- binding 1,6-di-O-phosphono-D-fructose: S49 (≠ T50), D82 (= D82), H83 (= H83), H165 (= H180), K169 (vs. gap), G196 (= G209), S198 (= S211), N238 (= N230), D240 (≠ A232), T241 (= T233), R244 (≠ K236)
- binding zinc ion: H83 (= H83), H83 (= H83), H83 (= H83), E134 (= E134), H165 (= H180), H165 (= H180), H165 (= H180), H195 (= H208), H195 (= H208)
5ud4A Class ii fructose-1,6-bisphosphate aldolase h180q variant of helicobacter pylori with tbp (see paper)
34% identity, 99% coverage: 3:284/284 of query aligns to 2:293/293 of 5ud4A
- binding 1,6-di-O-phosphono-D-tagatose: S49 (≠ T50), D82 (= D82), Q166 (≠ H180), G167 (= G181), K170 (vs. gap), G197 (= G209), S199 (= S211), N239 (= N230), D241 (≠ A232), T242 (= T233), R245 (≠ K236)
- binding zinc ion: H83 (= H83), H83 (= H83), E134 (= E134), H196 (= H208), H196 (= H208)
2isvB Structure of giardia fructose-1,6-biphosphate aldolase in complex with phosphoglycolohydroxamate (see paper)
35% identity, 98% coverage: 7:283/284 of query aligns to 6:291/307 of 2isvB
- binding phosphoglycolohydroxamic acid: D82 (= D82), H168 (= H180), G169 (= G181), K172 (≠ A184), H195 (= H208), G196 (= G209), S198 (= S211), N238 (= N230), D240 (≠ A232), S241 (≠ T233)
- binding zinc ion: H83 (= H83), H168 (= H180), H195 (= H208)
Query Sequence
>BWI76_RS24855 FitnessBrowser__Koxy:BWI76_RS24855
MFIISSKNMLLKAQRLGYAVPAFNIHNLETMQVVVETAAELRSPLILAGTPGTYSYAGTG
NVVAIARDLAKIWDLPLAVHLDHHEDLADITRKVQAGIRSVMIDGSHSPFEENVALVKSV
VELSHRYDASVEAELGRLGGVEDDLVVDAKDALYTNPEQAREFVARTGIDSLAVAIGTAH
GLYAAEPKLDFDRLAAIRDRVDVPLVLHGASGLPDSDIRRAISLGVCKVNVATELKIAFS
DALKHYFEENPDANDPRHYMKPAKAAMKDVVRKVIHVCGCEGQL
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SitesBLAST's Database
SitesBLAST's database includes
(1) SwissProt
entries with experimentally-supported functional features;
and (2) protein structures with bound ligands, from the
BioLip database.
by Morgan Price,
Arkin group
Lawrence Berkeley National Laboratory